MD14G1228500.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016847
Physical Location & Seq
Forward (+)
30972379 .. 30972996
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1228500.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGTGCAAGCAGAGCGTCGCCTATTAGCCAATGCTTTGCTTGACTTCTCCAACCAACGTTTTGTGCTCCTCTCAGAGTCATGCATACCACTCTTTAACTTCAGCATAGTCTACAACTATCTCATGGACTCAAAGCAAACGTTCGTGGACGCCTACGACCTTCCCGGCCCTGTCGGCCGAGACCGGTACAGGCACGAAATGAGGCCTCGTATCATACTTGATCAGTGGCGAAAAGGCTCACAGTGGTTCCAAGTGGACCGTGAGATCGCGACCGGAATCGTTTCGGATGAAAAGTACTTCCCGTTATTCACAAGGTATTGCATACCCCCATGCTACGCTGATGAGCACTACTTGCCCACATACGTAAGCATCAAGTTTTGGAAGAAGAATTCTAACAGGACGTTGACTTGGGTTGACTGGTCGAGGGGTGGGCCCCATCCGTCTAAGTTTATGAGAACAGATGTAACGATTGACTTCTTGAAGAAGTTGAGACATGGAAGCAACTGTGAGTACAATGGGAAGCCAACCAATATTTGCTATTTGTTTGCAAGGAAGTTCATGCTACACTCTTTAGATAGGCTGTTGAGGTTTGCTCCAAAGATCATGAGATTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

206

Amino Acids

24.49

Weight (kDa)

9.55

Isoelectric Point (pI)

21.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 1 - 164 3.9e-55 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016847)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11810
malus_domestica MD14G1228400.v1.1 MD14G1228500.v1.1
prunus_persica Prupe.5G223500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0185791
rosa_laevigata RLG00000004883
rosa_multiflora Rmu_co8078508.1_g000001 Rmu_sc0002121.1_g000003
rosa_rugosa Rorug06G0471300
rosa_samantha Rh7AG071000 Rh7BG076800 Rh7CG076800 Rh7DG076700
rosa_wichuraiana Rw7G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 269
AclI AACGTT 2 cut(s) 58, 140
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 3 cut(s) 188, 296, 512
AgeI ACCGGT 1 cut(s) 183
AgsI TTSAA 2 cut(s) 481, 613
Alw21I GWGCWC 2 cut(s) 69, 348
Alw26I GTCTC 2 cut(s) 174, 484
AoxI GGCC 4 cut(s) 166, 175, 203, 431
ApaI GGGCCC 1 cut(s) 435
ApoI RAATTY 1 cut(s) 388
AsiGI ACCGGT 1 cut(s) 183
Asp700I GAANNNNTTC 1 cut(s) 280
AspS9I GGNCC 4 cut(s) 167, 256, 431, 432
AsuC2I CCSGG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 435
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 2 cut(s) 69, 348
BccI CCATC 1 cut(s) 444
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 174, 484
BglI GCCNNNNNGGC 1 cut(s) 174
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 4 cut(s) 167, 256, 431, 432
BmiI GGNNCC 3 cut(s) 248, 433, 434
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 1 cut(s) 378
BplI GAGNNNNNCTC 2 cut(s) 577, 609
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 364
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 174
BsaWI WCCGGW 2 cut(s) 183, 272
Bse118I RCCGGY 1 cut(s) 183
Bse1I ACTGG 1 cut(s) 422
BseGI GGATG 2 cut(s) 292, 436
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 1 cut(s) 435
BseX3I CGGCCG 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 269
Bsh1285I CGRYCG 2 cut(s) 178, 273
BshFI GGCC 4 cut(s) 168, 177, 205, 433
BshTI ACCGGT 1 cut(s) 183
BsiEI CGRYCG 2 cut(s) 178, 273
BsiHKAI GWGCWC 2 cut(s) 69, 348
BsiSI CCGG 3 cut(s) 165, 184, 273
BsmAI GTCTC 2 cut(s) 174, 484
BsnI GGCC 4 cut(s) 168, 177, 205, 433
Bso31I GGTCTC 1 cut(s) 174
Bsp120I GGGCCC 1 cut(s) 431
Bsp1286I GDGCHC 3 cut(s) 69, 348, 435
Bsp143I GATC 3 cut(s) 220, 264, 600
Bsp68I TCGCGA 1 cut(s) 269
BspANI GGCC 4 cut(s) 168, 177, 205, 433
BspCNI CTCAG 1 cut(s) 86
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 1 cut(s) 603
BspLI GGNNCC 3 cut(s) 248, 433, 434
BspTNI GGTCTC 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 183
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 183
BssMI GATC 3 cut(s) 220, 264, 600
BssNI GRCGYC 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 243, 260, 506
BstACI GRCGYC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 352
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 2 cut(s) 73, 444
BstF5I GGATG 2 cut(s) 292, 436
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 3 cut(s) 223, 267, 603
BstMAI GTCTC 2 cut(s) 174, 484
BstMBI GATC 3 cut(s) 220, 264, 600
BstMCI CGRYCG 2 cut(s) 178, 273
BstMWI GCNNNNNNNGC 3 cut(s) 13, 174, 352
BstSCI CCNGG 1 cut(s) 163
BstSLI GKGCMC 1 cut(s) 435
BstSNI TACGTA 1 cut(s) 364
BstUI CGCG 1 cut(s) 269
BstZI CGGCCG 1 cut(s) 175
BsuRI GGCC 4 cut(s) 168, 177, 205, 433
BtsCI GGATG 2 cut(s) 292, 436
BtsIMutI CAGTG 2 cut(s) 230, 248
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 9
CciI TCATGA 1 cut(s) 603
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 4 cut(s) 167, 256, 431, 432
CseI GACGC 2 cut(s) 5, 158
Csp6I GTAC 3 cut(s) 187, 295, 511
CspAI ACCGGT 1 cut(s) 183
CviAII CATG 6 cut(s) 81, 124, 330, 494, 559, 604
CviJI RGCY 8 cut(s) 29, 168, 177, 205, 237, 433, 523, 580
CviKI_1 RGCY 8 cut(s) 29, 168, 177, 205, 237, 433, 523, 580
CviQI GTAC 3 cut(s) 187, 295, 511
DdeI CTNAG 2 cut(s) 73, 444
DpnI GATC 3 cut(s) 222, 266, 602
DpnII GATC 3 cut(s) 220, 264, 600
EaeI YGGCCR 1 cut(s) 175
EagI CGGCCG 1 cut(s) 175
EclXI CGGCCG 1 cut(s) 175
Eco105I TACGTA 1 cut(s) 364
Eco147I AGGCCT 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 256
Eco52I CGGCCG 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 435
FaeI CATG 6 cut(s) 84, 127, 333, 497, 562, 607
FatI CATG 6 cut(s) 80, 123, 329, 493, 558, 603
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 111
FokI GGATG 2 cut(s) 299, 423
FriOI GRGCYC 1 cut(s) 435
HaeIII GGCC 4 cut(s) 168, 177, 205, 433
HapII CCGG 3 cut(s) 165, 184, 273
HgaI GACGC 2 cut(s) 5, 158
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 6 cut(s) 84, 127, 333, 497, 562, 607
HincII GTYRAC 2 cut(s) 405, 415
HindII GTYRAC 2 cut(s) 405, 415
HinfI GANTC 4 cut(s) 77, 128, 276, 609
HpaII CCGG 3 cut(s) 165, 184, 273
Hpy166II GTNNAC 5 cut(s) 112, 148, 256, 405, 415
Hpy188I TCNGA 2 cut(s) 76, 286
Hpy188III TCNNGA 3 cut(s) 268, 478, 604
Hpy8I GTNNAC 5 cut(s) 112, 148, 256, 405, 415
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 3 cut(s) 243, 260, 506
HpyCH4IV ACGT 4 cut(s) 58, 140, 363, 401
HpyCH4V TGCA 4 cut(s) 7, 84, 321, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 174, 352
HpyF3I CTNAG 2 cut(s) 73, 444
HpySE526I ACGT 4 cut(s) 58, 140, 363, 401
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 6 cut(s) 84, 127, 333, 497, 562, 607
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 220, 264, 600
LmnI GCTCC 2 cut(s) 72, 598
LpnPI CCDG 7 cut(s) 175, 178, 183, 197, 286, 382, 403
LweI GCATC 1 cut(s) 378
MaeII ACGT 4 cut(s) 58, 140, 363, 401
MaeIII GTNAC 1 cut(s) 463
MalI GATC 3 cut(s) 222, 266, 602
MboI GATC 3 cut(s) 220, 264, 600
MboII GAAGA 3 cut(s) 394, 397, 493
MhlI GDGCHC 3 cut(s) 69, 348, 435
MluCI AATT 1 cut(s) 388
MlyI GAGTC 2 cut(s) 86, 122
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 5 cut(s) 80, 195, 216, 417, 579
Mph1103I ATGCAT 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 280
MseI TTAA 1 cut(s) 96
MspI CCGG 3 cut(s) 165, 184, 273
MspR9I CCNGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 3 cut(s) 13, 174, 352
NciI CCSGG 1 cut(s) 165
NdeII GATC 3 cut(s) 220, 264, 600
NlaIII CATG 6 cut(s) 84, 127, 333, 497, 562, 607
NlaIV GGNNCC 3 cut(s) 248, 433, 434
NmeAIII GCCGAG 1 cut(s) 203
NruI TCGCGA 1 cut(s) 269
NsiI ATGCAT 1 cut(s) 86
PagI TCATGA 1 cut(s) 603
PceI AGGCCT 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 280
PfeI GAWTC 2 cut(s) 276, 609
PinAI ACCGGT 1 cut(s) 183
PleI GAGTC 2 cut(s) 85, 122
PpsI GAGTC 2 cut(s) 85, 122
Ppu21I YACGTR 1 cut(s) 364
Psp1406I AACGTT 2 cut(s) 58, 140
PspN4I GGNNCC 3 cut(s) 248, 433, 434
PspOMI GGGCCC 1 cut(s) 431
PspPI GGNCC 4 cut(s) 167, 256, 431, 432
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 3 cut(s) 188, 296, 512
RsaNI GTAC 3 cut(s) 187, 295, 511
SaqAI TTAA 1 cut(s) 96
Sau3AI GATC 3 cut(s) 220, 264, 600
Sau96I GGNCC 4 cut(s) 167, 256, 431, 432
ScaI AGTACT 1 cut(s) 296
SchI GAGTC 2 cut(s) 86, 122
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 3 cut(s) 69, 348, 435
SetI ASST 7 cut(s) 61, 143, 162, 317, 366, 404, 590
SfaNI GCATC 1 cut(s) 378
SfiI GGCCNNNNNGGCC 1 cut(s) 174
SinI GGWCC 1 cut(s) 256
SnaBI TACGTA 1 cut(s) 364
Sse9I AATT 1 cut(s) 388
SseBI AGGCCT 1 cut(s) 205
SspI AATATT 1 cut(s) 532
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 3 cut(s) 243, 260, 506
TaiI ACGT 4 cut(s) 61, 143, 366, 404
TaqI TCGA 1 cut(s) 422
TasI AATT 1 cut(s) 388
TatI WGTACW 2 cut(s) 294, 510
TfiI GAWTC 2 cut(s) 276, 609
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 2 cut(s) 230, 248
TspDTI ATGAA 2 cut(s) 303, 547
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 2 cut(s) 230, 248
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 280
ZrmI AGTACT 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.