RchiOBHm_Chr7g0185791

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0016847
Physical Location & Seq
Forward (+)
5878311 .. 5878961
651 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16583

Sequence Viewer

Length: 651 bp
ATGTTGCAGAATGTGACGTGGGGTGAGCCAAACATGGTGCAAGCAGAGCGCCGCCTGCTAGCCAATGCACTGCTGGACTTGTCCAACCAACGTTTCATTCTTCTCTCGGAGTCGTGCATACCACTATTCAACTTCAAAACGGTCTATGATTACGTTATGGACTCAACCAAAACATTTGTGGAGGCCTACGACTTGCCCGGTCCAGTGGGCCGAGACAGATACAGGAAACAAATGAGCCCAACAATCAAGCTTGAGCAATGGCGGAAGGGGTCGCAGTGGTTCGAGGTGGACCGCCAGTTGGCACTGGAGGTGGTTTCGGACAGACGGTATTTTCCGCTGTTTGAGAAGTACTGCGTCCCCTCGTGCTACTCGGATGAGCACTACTTGCCTACTTACATGAGCATCAAATTTTGGAAGAAAAACTCGAACCGGACTTTGCATTGGGTTGACTGGTCGAGAGGGGGGCCACATCCGTCGAGGTATCAGAGGACGGATGTGACTCATGAGTTCTTGAGCAAGATGAGATTTGGAACAACATGCGAGTACAATGGGAACAACACTAACATATGTCATTTGTTTGCAAGAAAGTTCATGCCTAACACTTTGGATAGGCTTTTGAGGTTTGCTCCCAAGCTCATGCAATTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

216

Amino Acids

25.81

Weight (kDa)

9.17

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 3 - 175 3.4e-63 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016847)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11810
malus_domestica MD14G1228400.v1.1 MD14G1228500.v1.1
prunus_persica Prupe.5G223500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0185791
rosa_laevigata RLG00000004883
rosa_multiflora Rmu_co8078508.1_g000001 Rmu_sc0002121.1_g000003
rosa_rugosa Rorug06G0471300
rosa_samantha Rh7AG071000 Rh7BG076800 Rh7CG076800 Rh7DG076700
rosa_wichuraiana Rw7G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 52, 262, 292, 335
AclI AACGTT 1 cut(s) 91
AcsI RAATTY 1 cut(s) 407
AfaI GTAC 2 cut(s) 350, 545
AfiI CCNNNNNNNGG 1 cut(s) 298
AgsI TTSAA 2 cut(s) 130, 136
AjiI CACGTC 1 cut(s) 18
AjuI GAANNNNNNNTTGG 4 cut(s) 77, 81, 109, 113
AluBI AGCT 2 cut(s) 250, 634
AluI AGCT 2 cut(s) 250, 634
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 207
AoxI GGCC 3 cut(s) 183, 208, 464
ApoI RAATTY 1 cut(s) 407
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 4 cut(s) 200, 208, 289, 464
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 1 cut(s) 35
AsuNHI GCTAGC 1 cut(s) 58
AvaII GGWCC 2 cut(s) 200, 289
BanII GRGCYC 1 cut(s) 239
BauI CACGAG 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 381
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 1 cut(s) 59
BfoI RGCGCY 1 cut(s) 52
BisI GCNGC 1 cut(s) 52
BlsI GCNGC 1 cut(s) 53
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 1 cut(s) 198
Bme18I GGWCC 2 cut(s) 200, 289
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 4 cut(s) 200, 208, 289, 464
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 198
BmsI GCATC 1 cut(s) 411
BmtI GCTAGC 1 cut(s) 62
BplI GAGNNNNNCTC 2 cut(s) 610, 642
BpmI CTGGAG 1 cut(s) 326
BpuEI CTTGAG 2 cut(s) 272, 532
BpuMI CCSGG 1 cut(s) 198
BsaWI WCCGGW 1 cut(s) 429
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 4 cut(s) 203, 295, 309, 455
Bse3DI GCAATG 1 cut(s) 263
BseGI GGATG 3 cut(s) 379, 469, 499
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 1 cut(s) 263
BseNI ACTGG 4 cut(s) 203, 295, 309, 455
BshFI GGCC 3 cut(s) 185, 210, 466
BsiHKAI GWGCWC 1 cut(s) 381
BsiSI CCGG 2 cut(s) 198, 430
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 3 cut(s) 185, 210, 466
Bsp1286I GDGCHC 2 cut(s) 239, 381
BspACI CCGC 4 cut(s) 52, 262, 292, 335
BspANI GGCC 3 cut(s) 185, 210, 466
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 465
BspOI GCTAGC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 263
BsrI ACTGG 4 cut(s) 203, 295, 309, 455
BssSI CACGAG 1 cut(s) 361
Bst2BI CACGAG 1 cut(s) 361
Bst4CI ACNGT 2 cut(s) 142, 327
BstAPI GCANNNNNTGC 1 cut(s) 385
BstC8I GCNNGC 3 cut(s) 42, 56, 60
BstF5I GGATG 3 cut(s) 379, 469, 499
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 1 cut(s) 51
BstMAI GTCTC 1 cut(s) 207
BstMWI GCNNNNNNNGC 3 cut(s) 46, 55, 385
BstNSI RCATGY 1 cut(s) 540
BstSCI CCNGG 1 cut(s) 196
BsuRI GGCC 3 cut(s) 185, 210, 466
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 3 cut(s) 379, 469, 499
BtsI GCAGTG 2 cut(s) 68, 281
BtsIMutI CAGTG 4 cut(s) 68, 210, 281, 302
Cac8I GCNNGC 3 cut(s) 42, 56, 60
CciI TCATGA 1 cut(s) 502
CfoI GCGC 1 cut(s) 51
Cfr13I GGNCC 4 cut(s) 200, 208, 289, 464
CseI GACGC 1 cut(s) 343
Csp6I GTAC 2 cut(s) 349, 544
CviAII CATG 6 cut(s) 34, 397, 503, 537, 592, 637
CviJI RGCY 9 cut(s) 28, 62, 185, 210, 237, 250, 466, 613, 634
CviKI_1 RGCY 9 cut(s) 28, 62, 185, 210, 237, 250, 466, 613, 634
CviQI GTAC 2 cut(s) 349, 544
EciI GGCGGA 1 cut(s) 277
Eco147I AGGCCT 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 200, 289
EcoT38I GRGCYC 1 cut(s) 239
FaeI CATG 6 cut(s) 37, 400, 506, 540, 595, 640
FaqI GGGAC 1 cut(s) 341
FatI CATG 6 cut(s) 33, 396, 502, 536, 591, 636
FauNDI CATATG 1 cut(s) 566
Fnu4HI GCNGC 1 cut(s) 52
FokI GGATG 3 cut(s) 386, 456, 506
FriOI GRGCYC 1 cut(s) 239
Fsp4HI GCNGC 1 cut(s) 52
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 50
GluI GCNGC 1 cut(s) 52
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 3 cut(s) 185, 210, 466
HapII CCGG 2 cut(s) 198, 430
HgaI GACGC 1 cut(s) 343
HhaI GCGC 1 cut(s) 51
Hin1II CATG 6 cut(s) 37, 400, 506, 540, 595, 640
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 3 cut(s) 110, 161, 499
HpaII CCGG 2 cut(s) 198, 430
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 2 cut(s) 289, 448
Hpy188I TCNGA 4 cut(s) 109, 319, 373, 486
Hpy188III TCNNGA 3 cut(s) 456, 503, 511
Hpy8I GTNNAC 2 cut(s) 289, 448
Hpy99I CGWCG 1 cut(s) 478
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 2 cut(s) 142, 327
HpyCH4IV ACGT 3 cut(s) 17, 91, 153
HpyCH4V TGCA 7 cut(s) 7, 40, 68, 117, 439, 581, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 55, 385
HpySE526I ACGT 3 cut(s) 17, 91, 153
Hsp92II CATG 6 cut(s) 37, 400, 506, 540, 595, 640
HspAI GCGC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 631
LpnPI CCDG 9 cut(s) 59, 68, 208, 211, 216, 290, 308, 436, 443
LweI GCATC 1 cut(s) 411
MaeI CTAG 1 cut(s) 59
MaeII ACGT 3 cut(s) 17, 91, 153
MaeIII GTNAC 2 cut(s) 13, 496
MboII GAAGA 2 cut(s) 92, 427
MhlI GDGCHC 2 cut(s) 239, 381
MluCI AATT 2 cut(s) 407, 641
MlyI GAGTC 3 cut(s) 119, 155, 493
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 8 cut(s) 175, 277, 301, 370, 452, 471, 480, 612
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 2 cut(s) 198, 430
MspR9I CCNGG 1 cut(s) 198
MwoI GCNNNNNNNGC 3 cut(s) 46, 55, 385
NciI CCSGG 1 cut(s) 198
NdeI CATATG 1 cut(s) 566
NheI GCTAGC 1 cut(s) 58
NlaIII CATG 6 cut(s) 37, 400, 506, 540, 595, 640
NlaIV GGNNCC 1 cut(s) 465
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 2 cut(s) 13, 496
NspI RCATGY 1 cut(s) 540
PagI TCATGA 1 cut(s) 502
PceI AGGCCT 1 cut(s) 185
PkrI GCNGC 1 cut(s) 53
PleI GAGTC 3 cut(s) 118, 155, 493
PpsI GAGTC 3 cut(s) 118, 155, 493
Psp1406I AACGTT 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 4 cut(s) 200, 208, 289, 464
RsaI GTAC 2 cut(s) 350, 545
RsaNI GTAC 2 cut(s) 349, 544
SatI GCNGC 1 cut(s) 52
Sau96I GGNCC 4 cut(s) 200, 208, 289, 464
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 3 cut(s) 119, 155, 493
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 2 cut(s) 239, 381
SetI ASST 9 cut(s) 20, 94, 156, 252, 288, 312, 482, 623, 636
SfaNI GCATC 1 cut(s) 411
SinI GGWCC 2 cut(s) 200, 289
SmlI CTYRAG 2 cut(s) 251, 511
SmoI CTYRAG 2 cut(s) 251, 511
Sse9I AATT 2 cut(s) 407, 641
SseBI AGGCCT 1 cut(s) 185
SsiI CCGC 4 cut(s) 52, 262, 292, 335
SspMI CTAG 1 cut(s) 59
StuI AGGCCT 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 196
TaaI ACNGT 2 cut(s) 142, 327
TaiI ACGT 3 cut(s) 20, 94, 156
TaqI TCGA 5 cut(s) 282, 425, 455, 476, 645
TasI AATT 2 cut(s) 407, 641
TatI WGTACW 2 cut(s) 348, 543
TauI GCSGC 1 cut(s) 54
TscAI CASTG 4 cut(s) 75, 210, 281, 309
TseFI GTSAC 2 cut(s) 13, 496
Tsp45I GTSAC 2 cut(s) 13, 496
TspDTI ATGAA 2 cut(s) 85, 580
TspGWI ACGGA 2 cut(s) 462, 506
TspRI CASTG 4 cut(s) 75, 210, 281, 309
VpaK11BI GGWCC 2 cut(s) 200, 289
XapI RAATTY 1 cut(s) 407
XceI RCATGY 1 cut(s) 540
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 175
XspI CTAG 1 cut(s) 59
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.