MD14G1228400.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016847
Physical Location & Seq
Forward (+)
30936539 .. 30940687
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1228400.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGATGAATAAGCATCAGCAACACCACCCACCACCTTCATTTCCAAAGCACTTCCTCAATGCCCAAATCCACCTCCATAAAATCGTCTCAAGTTTTCTAATCTTTGCTTTCGGTTTAGCACTAGGCGCTTCCCTCAGCATCTACCTCAAAGATTTCCCCTTCCAACTTTATTCAACACAAAACAAATACTCTCATTTGTCAACAAATAATCTAACATTTTCTTCTCCACCACCTCCACAATCTCTCAATATCCCAACAAATAATCGAACATCTTCTTCTCCACCACGACCACCACAATCTCTCAATATCCCGACAAATCAAACAAGAGCCAAAACCTCCGCCGCCAGCCCTGTACCACCAATAGGGTTGAAAGAATACACTAAGTCGCCGAATATTTTGCATGAAATGGACGACGATGAGCTGTTGTGGAGAGCTTCTCTGGTGCCGCTACGGCGGGGTTTGCCATTTAAACGGACTCCAAAAGTTGCATTCATGTTTCTGACGAGAGGGAGCCTGGCAATGGCTCCTCTCTGGGAAATGTTCTTCAAAGGACATGAAGGGCTGTACTCCATTTATGTCCATGCCAATCCGGATTTCAATGACACCATACCTGAAAACTCGGTTTTTTATGGCAGGAGGGTTCCGAGTAAGAATGTGACATGGGGTCAACCTAACATGGTGCAAGCTGAGCGTCGCCTATTAGCCAATGCTTTGCTTGACTTCTCCAACCAACGTTTTGTGCTCCTCTCAGAGTCATGCATACCACTCTTTAACTTCAACATAGTCTACAATTATCTCATGGACTCAAACCAAACGTTCGTGGAGGCCTACGACCTTCCCGGCCCTGTCGGCCGAGACCGGTACAGGCACAAAATGAGGCCTCATATCATACTCGATCAGTGGCGAAAAGGCTCACAGTGGTTCGAAGTGGACCGTGAGATCGCGACCGCAATGGTTTCGGATGAAAAGTACTTCCCGTTATTCACAAGGTATTGCAAACCCCCATGCTACTCCGATGAGCACTACTTGCCCACATATGTAAGCATCAAGTTTTGGAAGAAGAATTCTAACAGGACGTTGACCTGGGTTGACTGGTCGAGGGGTGGGCCCCATCCGTCTAAGTTTATGAGAACAGATGTAACGATCGACTTCTTGAAGAAGCTGAGACATGGAAGCAACTGTGAGTACAATGGGAAGCCAACCAATATTTGCTTTTTGTTTGCAAGGAAGTTCTTGCCACACTCTTTAGATAGGCTGTTGAGGTTTGCTCCAAAGATCATGAGATTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

431

Amino Acids

49.95

Weight (kDa)

9.77

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 162 - 389 4.6e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016847)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11810
malus_domestica MD14G1228400.v1.1 MD14G1228500.v1.1
prunus_persica Prupe.5G223500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0185791
rosa_laevigata RLG00000004883
rosa_multiflora Rmu_co8078508.1_g000001 Rmu_sc0002121.1_g000003
rosa_rugosa Rorug06G0471300
rosa_samantha Rh7AG071000 Rh7BG076800 Rh7CG076800 Rh7DG076700
rosa_wichuraiana Rw7G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 442
AccI GTMKAC 1 cut(s) 786
AccII CGCG 1 cut(s) 944
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 5 cut(s) 339, 342, 446, 454, 948
AclI AACGTT 2 cut(s) 733, 815
AcoI YGGCCR 1 cut(s) 850
AcsI RAATTY 1 cut(s) 1063
AfaI GTAC 5 cut(s) 354, 566, 863, 971, 1187
AfiI CCNNNNNNNGG 2 cut(s) 362, 520
AgeI ACCGGT 1 cut(s) 858
AgsI TTSAA 7 cut(s) 174, 370, 547, 598, 778, 1156, 1288
AhdI GACNNNNNGTC 1 cut(s) 663
AjnI CCWGG 2 cut(s) 513, 1082
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AluBI AGCT 4 cut(s) 421, 434, 686, 1162
AluI AGCT 4 cut(s) 421, 434, 686, 1162
Alw21I GWGCWC 2 cut(s) 744, 1023
Alw26I GTCTC 3 cut(s) 91, 849, 1159
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 5 cut(s) 825, 841, 850, 878, 1106
ApaI GGGCCC 1 cut(s) 1110
ApoI RAATTY 1 cut(s) 1063
AsiGI ACCGGT 1 cut(s) 858
Asp700I GAANNNNTTC 1 cut(s) 271
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 4 cut(s) 842, 931, 1106, 1107
AsuC2I CCSGG 1 cut(s) 840
AsuII TTCGAA 1 cut(s) 924
AvaII GGWCC 1 cut(s) 931
BaeGI GKGCMC 1 cut(s) 1110
BanI GGYRCC 1 cut(s) 442
BanII GRGCYC 1 cut(s) 1110
Bbv12I GWGCWC 2 cut(s) 744, 1023
BbvCI CCTCAGC 1 cut(s) 134
BccI CCATC 1 cut(s) 1119
BceAI ACGGC 1 cut(s) 467
BcgI CGANNNNNNTGC 4 cut(s) 379, 413, 939, 973
BciT130I CCWGG 2 cut(s) 515, 1084
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 3 cut(s) 91, 849, 1159
BfaI CTAG 1 cut(s) 122
BfoI RGCGCY 1 cut(s) 129
BglI GCCNNNNNGGC 2 cut(s) 451, 849
BisI GCNGC 2 cut(s) 342, 446
BlpI GCTNAGC 1 cut(s) 687
BlsI GCNGC 2 cut(s) 343, 447
BmcAI AGTACT 1 cut(s) 971
Bme1390I CCNGG 3 cut(s) 515, 840, 1084
Bme18I GGWCC 1 cut(s) 931
BmeRI GACNNNNNGTC 1 cut(s) 663
BmgT120I GGNCC 4 cut(s) 842, 931, 1106, 1107
BmiI GGNNCC 6 cut(s) 444, 512, 525, 642, 1108, 1109
BmrFI CCNGG 3 cut(s) 515, 840, 1084
BmsI GCATC 3 cut(s) 22, 147, 1053
BplI GAGNNNNNCTC 4 cut(s) 421, 453, 1252, 1284
Bpu10I CCTNAGC 1 cut(s) 134
Bpu1102I GCTNAGC 1 cut(s) 687
Bpu14I TTCGAA 1 cut(s) 924
BpuEI CTTGAG 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 840
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 1 cut(s) 1083
BsaWI WCCGGW 2 cut(s) 589, 858
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 520
Bse118I RCCGGY 1 cut(s) 858
Bse1I ACTGG 1 cut(s) 1097
Bse3DI GCAATG 2 cut(s) 525, 957
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 2 cut(s) 515, 1084
BseDI CCNNGG 1 cut(s) 1083
BseGI GGATG 2 cut(s) 967, 1111
BseLI CCNNNNNNNGG 2 cut(s) 362, 520
BseMI GCAATG 2 cut(s) 525, 957
BseMII CTCAG 4 cut(s) 148, 678, 762, 1154
BseNI ACTGG 1 cut(s) 1097
BseRI GAGGAG 2 cut(s) 516, 734
BseSI GKGCMC 1 cut(s) 1110
BseX3I CGGCCG 1 cut(s) 850
Bsh1236I CGCG 1 cut(s) 944
Bsh1285I CGRYCG 3 cut(s) 853, 948, 1146
BshFI GGCC 5 cut(s) 827, 843, 852, 880, 1108
BshNI GGYRCC 1 cut(s) 442
BshTI ACCGGT 1 cut(s) 858
BsiEI CGRYCG 3 cut(s) 853, 948, 1146
BsiHKAI GWGCWC 2 cut(s) 744, 1023
BsiSI CCGG 3 cut(s) 590, 840, 859
BslI CCNNNNNNNGG 2 cut(s) 362, 520
BsmAI GTCTC 3 cut(s) 91, 849, 1159
BsmBI CGTCTC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 5 cut(s) 827, 843, 852, 880, 1108
Bso31I GGTCTC 1 cut(s) 849
Bsp119I TTCGAA 1 cut(s) 924
Bsp120I GGGCCC 1 cut(s) 1106
Bsp1286I GDGCHC 3 cut(s) 744, 1023, 1110
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 4 cut(s) 895, 939, 1143, 1275
Bsp1720I GCTNAGC 1 cut(s) 687
Bsp68I TCGCGA 1 cut(s) 944
BspACI CCGC 5 cut(s) 339, 342, 446, 454, 948
BspANI GGCC 5 cut(s) 827, 843, 852, 880, 1108
BspCNI CTCAG 4 cut(s) 147, 679, 761, 1155
BspEI TCCGGA 1 cut(s) 589
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 1 cut(s) 1278
BspLI GGNNCC 6 cut(s) 444, 512, 525, 642, 1108, 1109
BspT104I TTCGAA 1 cut(s) 924
BspT107I GGYRCC 1 cut(s) 442
BspTNI GGTCTC 1 cut(s) 849
BsrDI GCAATG 2 cut(s) 525, 957
BsrFI RCCGGY 1 cut(s) 858
BsrI ACTGG 1 cut(s) 1097
BssAI RCCGGY 1 cut(s) 858
BssECI CCNNGG 1 cut(s) 1083
BssMI GATC 4 cut(s) 895, 939, 1143, 1275
Bst2UI CCWGG 2 cut(s) 515, 1084
Bst4CI ACNGT 3 cut(s) 918, 935, 1181
BstAPI GCANNNNNTGC 1 cut(s) 1027
BstBI TTCGAA 1 cut(s) 924
BstC8I GCNNGC 2 cut(s) 346, 684
BstDEI CTNAG 6 cut(s) 134, 381, 687, 748, 1119, 1163
BstF5I GGATG 2 cut(s) 967, 1111
BstFNI CGCG 1 cut(s) 944
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 4 cut(s) 898, 942, 1146, 1278
BstMAI GTCTC 3 cut(s) 91, 849, 1159
BstMBI GATC 4 cut(s) 895, 939, 1143, 1275
BstMCI CGRYCG 3 cut(s) 853, 948, 1146
BstMWI GCNNNNNNNGC 6 cut(s) 125, 451, 460, 688, 849, 1027
BstNI CCWGG 2 cut(s) 515, 1084
BstSCI CCNGG 3 cut(s) 513, 838, 1082
BstSLI GKGCMC 1 cut(s) 1110
BstUI CGCG 1 cut(s) 944
BstZI CGGCCG 1 cut(s) 850
BsuRI GGCC 5 cut(s) 827, 843, 852, 880, 1108
BtsCI GGATG 2 cut(s) 967, 1111
BtsIMutI CAGTG 2 cut(s) 905, 923
BtuMI TCGCGA 1 cut(s) 944
Cac8I GCNNGC 2 cut(s) 346, 684
CciI TCATGA 1 cut(s) 1278
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 858
Cfr13I GGNCC 4 cut(s) 842, 931, 1106, 1107
CseI GACGC 1 cut(s) 680
Csp6I GTAC 5 cut(s) 353, 565, 862, 970, 1186
CspAI ACCGGT 1 cut(s) 858
CviQI GTAC 5 cut(s) 353, 565, 862, 970, 1186
DdeI CTNAG 6 cut(s) 134, 381, 687, 748, 1119, 1163
DpnI GATC 4 cut(s) 897, 941, 1145, 1277
DpnII GATC 4 cut(s) 895, 939, 1143, 1275
DraI TTTAAA 1 cut(s) 469
DriI GACNNNNNGTC 1 cut(s) 663
EaeI YGGCCR 1 cut(s) 850
EagI CGGCCG 1 cut(s) 850
Eam1105I GACNNNNNGTC 1 cut(s) 663
EciI GGCGGA 1 cut(s) 328
EclXI CGGCCG 1 cut(s) 850
Eco147I AGGCCT 2 cut(s) 827, 880
Eco24I GRGCYC 1 cut(s) 1110
Eco31I GGTCTC 1 cut(s) 849
Eco47I GGWCC 1 cut(s) 931
Eco52I CGGCCG 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 1107
EcoRI GAATTC 1 cut(s) 1063
EcoRII CCWGG 2 cut(s) 513, 1082
EcoT22I ATGCAT 1 cut(s) 761
EcoT38I GRGCYC 1 cut(s) 1110
Esp3I CGTCTC 1 cut(s) 91
FauI CCCGC 1 cut(s) 447
FauNDI CATATG 1 cut(s) 1036
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 2 cut(s) 342, 446
FokI GGATG 2 cut(s) 974, 1098
FriOI GRGCYC 1 cut(s) 1110
Fsp4HI GCNGC 2 cut(s) 342, 446
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 127
GluI GCNGC 2 cut(s) 342, 446
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 5 cut(s) 827, 843, 852, 880, 1108
HapII CCGG 3 cut(s) 590, 840, 859
HgaI GACGC 1 cut(s) 680
HhaI GCGC 1 cut(s) 128
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 4 cut(s) 201, 668, 1080, 1090
HindII GTYRAC 4 cut(s) 201, 668, 1080, 1090
HinfI GANTC 4 cut(s) 475, 752, 803, 1284
HpaII CCGG 3 cut(s) 590, 840, 859
Hpy166II GTNNAC 6 cut(s) 201, 668, 787, 931, 1080, 1090
Hpy188I TCNGA 5 cut(s) 501, 645, 751, 961, 1015
Hpy188III TCNNGA 5 cut(s) 310, 590, 943, 1153, 1279
Hpy8I GTNNAC 6 cut(s) 201, 668, 787, 931, 1080, 1090
Hpy99I CGWCG 2 cut(s) 416, 696
HpyAV CCTTC 4 cut(s) 45, 169, 551, 845
HpyCH4III ACNGT 3 cut(s) 918, 935, 1181
HpyCH4IV ACGT 3 cut(s) 733, 815, 1076
HpyCH4V TGCA 6 cut(s) 400, 488, 682, 759, 996, 1223
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 451, 460, 688, 849, 1027
HpyF3I CTNAG 6 cut(s) 134, 381, 687, 748, 1119, 1163
HpySE526I ACGT 3 cut(s) 733, 815, 1076
HspAI GCGC 1 cut(s) 126
Kpn2I TCCGGA 1 cut(s) 589
Kzo9I GATC 4 cut(s) 895, 939, 1143, 1275
LmnI GCTCC 4 cut(s) 510, 529, 747, 1273
LweI GCATC 3 cut(s) 22, 147, 1053
MaeI CTAG 1 cut(s) 122
MaeII ACGT 3 cut(s) 733, 815, 1076
MaeIII GTNAC 2 cut(s) 655, 1138
MalI GATC 4 cut(s) 897, 941, 1145, 1277
MboI GATC 4 cut(s) 895, 939, 1143, 1275
MboII GAAGA 7 cut(s) 213, 264, 267, 535, 1069, 1072, 1168
MfeI CAATTG 1 cut(s) 1288
MhlI GDGCHC 3 cut(s) 744, 1023, 1110
MluCI AATT 3 cut(s) 790, 1063, 1288
MlyI GAGTC 3 cut(s) 469, 761, 797
MmeI TCCRAC 2 cut(s) 187, 750
Mph1103I ATGCAT 1 cut(s) 761
MroI TCCGGA 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 271
MseI TTAA 2 cut(s) 468, 771
MspI CCGG 3 cut(s) 590, 840, 859
MspR9I CCNGG 3 cut(s) 515, 840, 1084
MunI CAATTG 1 cut(s) 1288
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 2 cut(s) 515, 1084
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 6 cut(s) 125, 451, 460, 688, 849, 1027
NciI CCSGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 1036
NdeII GATC 4 cut(s) 895, 939, 1143, 1275
NlaIV GGNNCC 6 cut(s) 444, 512, 525, 642, 1108, 1109
NmeAIII GCCGAG 1 cut(s) 878
NmuCI GTSAC 1 cut(s) 655
NruI TCGCGA 1 cut(s) 944
NsiI ATGCAT 1 cut(s) 761
NspV TTCGAA 1 cut(s) 924
PagI TCATGA 1 cut(s) 1278
PceI AGGCCT 2 cut(s) 827, 880
PctI GAATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 271
PfeI GAWTC 1 cut(s) 1284
PinAI ACCGGT 1 cut(s) 858
PkrI GCNGC 2 cut(s) 343, 447
Ple19I CGATCG 1 cut(s) 1146
PleI GAGTC 3 cut(s) 469, 760, 797
PpsI GAGTC 3 cut(s) 469, 760, 797
Psp1406I AACGTT 2 cut(s) 733, 815
Psp6I CCWGG 2 cut(s) 513, 1082
PspGI CCWGG 2 cut(s) 513, 1082
PspN4I GGNNCC 6 cut(s) 444, 512, 525, 642, 1108, 1109
PspOMI GGGCCC 1 cut(s) 1106
PspPI GGNCC 4 cut(s) 842, 931, 1106, 1107
PvuI CGATCG 1 cut(s) 1146
RruI TCGCGA 1 cut(s) 944
RsaI GTAC 5 cut(s) 354, 566, 863, 971, 1187
RsaNI GTAC 5 cut(s) 353, 565, 862, 970, 1186
SaqAI TTAA 2 cut(s) 468, 771
SatI GCNGC 2 cut(s) 342, 446
Sau3AI GATC 4 cut(s) 895, 939, 1143, 1275
Sau96I GGNCC 4 cut(s) 842, 931, 1106, 1107
ScaI AGTACT 1 cut(s) 971
SchI GAGTC 3 cut(s) 469, 761, 797
ScrFI CCNGG 3 cut(s) 515, 840, 1084
SduI GDGCHC 3 cut(s) 744, 1023, 1110
SfaNI GCATC 3 cut(s) 22, 147, 1053
SfiI GGCCNNNNNGGCC 1 cut(s) 849
SfuI TTCGAA 1 cut(s) 924
SinI GGWCC 1 cut(s) 931
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
Sse9I AATT 3 cut(s) 790, 1063, 1288
SseBI AGGCCT 2 cut(s) 827, 880
SsiI CCGC 5 cut(s) 339, 342, 446, 454, 948
SspI AATATT 2 cut(s) 394, 1207
SspMI CTAG 1 cut(s) 122
StuI AGGCCT 2 cut(s) 827, 880
StyD4I CCNGG 3 cut(s) 513, 838, 1082
TaaI ACNGT 3 cut(s) 918, 935, 1181
TaiI ACGT 3 cut(s) 736, 818, 1079
TaqI TCGA 5 cut(s) 265, 894, 924, 1097, 1146
TasI AATT 3 cut(s) 790, 1063, 1288
TatI WGTACW 3 cut(s) 564, 969, 1185
TauI GCSGC 2 cut(s) 344, 448
TfiI GAWTC 1 cut(s) 1284
Tru1I TTAA 2 cut(s) 468, 771
Tru9I TTAA 2 cut(s) 468, 771
TscAI CASTG 2 cut(s) 905, 923
TseFI GTSAC 1 cut(s) 655
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 6 cut(s) 20, 27, 417, 481, 570, 978
TspGWI ACGGA 2 cut(s) 487, 1104
TspRI CASTG 2 cut(s) 905, 923
VpaK11BI GGWCC 1 cut(s) 931
XapI RAATTY 1 cut(s) 1063
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 271
XspI CTAG 1 cut(s) 122
ZrmI AGTACT 1 cut(s) 971
Zsp2I ATGCAT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.