FvH4_5g12590

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012263
Physical Location & Seq
Forward (+)
7103124 .. 7107034
3911 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12590.t1

Sequence Viewer

Length: 1287 bp
ATGCGCTGCGACGCTGCGTCCTCTTGGCTCTACGGCCGCCACGTGTCCGTCGCACCCGCCATACCCACCGCCGGAACATCTTTCCTCTTCCTCTGCAGACCCGCCGTCTCATGCTGCACCAACACTCCCAACCCACTCAAGCGTCGATACTCCGGTAGCAGTCATGCTCCGGACATCGTCCGCCGTTGGATCCAGTCCGATCATCCTCTAGCTTCTCAAGATAGGTTCACGGTTGCTTCGTACAATATCTTGGGGGATAGAAATGCTTTTGCTCATAGAGACATGTATGGGAATGTGCCGTCTATGTATCTGAAGTGGGACCACCGGAAAAGGGTCATATGCGATGAAATCTCTGCCTGGAACTCTCATATAGTTTGCTTGCAAGAGGTAGACAAGTATTTGGAGCTTTCGAACAATTTGGCAAAACTGGGATATGCTGGTTCTTATAAGAGACGCACAGGAGATACTGTTGATGGTTGCGCTACTTTCTGGAAATCTCATAATTTACGGTTGTTAGATGAAGAGAGCATTGAATTCAAGGGATACGGACTTCGTGACAATGTTGCCCAACTTTCGGTTTTTGAGATGCATAAAGACAAATCAAGAAAGTTGGTGATTGGTAACATCCATGTGCTCTACAGCCCTAGCCGAGGGGAAATTAAATTGGGCCAGATTCGTTTTCTCTTATCAAGGGCCCATATCCTCTCGGAGAGATGGGGTAATGCCCCTGTTGTCCTTTGTGGTGATTTCAATAGCACTCCTCAGAGTGCGATATACAAGTTCTTGTCAACATCTGAGCTAAATATCATGTCATATGACAGAACAGAGTTATCTGGGCAGAAGAGCTGTCATCCTGCTCAAGTTTTAGGAGTCAAACAAAGAACTAGCAGTCCATTGAACTTGATAGATGGATTATTGAAACATAGCTGGACAGATGAAGAGATTAGAGTTGCAACTGGAAATTCTGGCAGCCATTCAGTTGTGCATCCCTTGAAGCTTAACAGTTCTTACGCTATGGTGCAGGGTTCTACAGCAACACGGGGATCCAGTGGTGAGCCTTTGGCTACTTCCTACCATTCTAAGTTTCTTGGAACCGTTGACTACTTATGGTACTCGGATGGTCTTGTGCCGACAGGAGTTCTGGATACAGTTTCACTTGACACTCTCCAGAGGATTGGTAGCCTCCCTTGCAAGAAAGTGGGCAGTGATCACCTGGCTTTAGTTTCTGAGTTTGCCTTCACACCAGGCACAAAGGAAGGCAACAACACAACTGCATCAACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

429

Amino Acids

47.41

Weight (kDa)

8.89

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 79 - 404 3.5e-18 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18500 AT3G18500 AT3G18500 AT3G18500
fragaria_vesca FvH4_5g12590
malus_domestica MD06G1212300.v1.1
prunus_persica Prupe.5G218400_v2.0.a1
pyrus_communis pycom06g18950
rosa_chinensis RchiOBHm_Chr7g0184741
rosa_laevigata RLG00000004954
rosa_multiflora Rmu_co8486587.1_g000001 Rmu_ssc0000289.1_g000017
rosa_roxburghii Rroxscaffold_3G00269380
rosa_rugosa Rorug06G0464900
rosa_samantha Rh7AG078000 Rh7BG069300 Rh7CG070100 Rh7DG069500
rosa_wichuraiana Rw7G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccI GTMKAC 1 cut(s) 390
AccIII TCCGGA 1 cut(s) 169
AciI CCGC 5 cut(s) 37, 57, 69, 102, 181
AclWI GGATC 4 cut(s) 184, 197, 1038, 1051
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 2 cut(s) 533, 961
AcuI CTGAAG 1 cut(s) 332
AcvI CACGTG 1 cut(s) 43
AfaI GTAC 2 cut(s) 242, 1112
AfiI CCNNNNNNNGG 4 cut(s) 71, 331, 574, 650
AflIII ACRYGT 2 cut(s) 42, 282
AgsI TTSAA 6 cut(s) 533, 538, 751, 898, 919, 994
AhdI GACNNNNNGTC 2 cut(s) 16, 104
AjnI CCWGG 3 cut(s) 356, 1212, 1243
AloI GAACNNNNNNTCC 2 cut(s) 874, 906
AluBI AGCT 6 cut(s) 212, 406, 799, 846, 927, 997
AluI AGCT 6 cut(s) 212, 406, 799, 846, 927, 997
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 3 cut(s) 112, 273, 445
AlwI GGATC 4 cut(s) 184, 197, 1038, 1051
Aor13HI TCCGGA 1 cut(s) 169
AoxI GGCC 3 cut(s) 34, 667, 693
ApaI GGGCCC 1 cut(s) 697
ApeKI GCWGC 4 cut(s) 6, 14, 114, 969
ApoI RAATTY 2 cut(s) 533, 961
AspLEI GCGC 2 cut(s) 6, 482
AspS9I GGNCC 4 cut(s) 319, 667, 693, 694
AsuHPI GGTGA 4 cut(s) 625, 755, 1064, 1202
AsuII TTCGAA 1 cut(s) 410
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 1 cut(s) 697
BaeI ACNNNNGTAYC 2 cut(s) 139, 172
BamHI GGATCC 2 cut(s) 189, 1043
BanII GRGCYC 1 cut(s) 697
BbrPI CACGTG 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 2 cut(s) 101, 981
BccI CCATC 4 cut(s) 467, 708, 902, 1112
BceAI ACGGC 4 cut(s) 49, 89, 168, 283
BciT130I CCWGG 3 cut(s) 358, 1214, 1245
BciVI GTATCC 2 cut(s) 536, 1138
BclI TGATCA 1 cut(s) 1207
BcoDI GTCTC 3 cut(s) 112, 273, 445
BfaI CTAG 3 cut(s) 209, 645, 885
BfmI CTRYAG 3 cut(s) 94, 637, 1029
BfuI GTATCC 2 cut(s) 536, 1138
BisI GCNGC 5 cut(s) 7, 15, 37, 115, 970
BlsI GCNGC 5 cut(s) 8, 16, 38, 116, 971
Bme1390I CCNGG 3 cut(s) 358, 1214, 1245
Bme18I GGWCC 1 cut(s) 319
BmeRI GACNNNNNGTC 2 cut(s) 16, 104
BmgT120I GGNCC 4 cut(s) 319, 667, 693, 694
BmiI GGNNCC 5 cut(s) 191, 320, 695, 1045, 1093
BmrFI CCNGG 3 cut(s) 358, 1214, 1245
BmrI ACTGGG 1 cut(s) 437
BmsI GCATC 3 cut(s) 576, 994, 1283
BmuI ACTGGG 1 cut(s) 437
BpmI CTGGAG 1 cut(s) 1151
Bpu14I TTCGAA 1 cut(s) 410
BpuEI CTTGAG 3 cut(s) 122, 201, 843
BsaAI YACGTR 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 649
BsaWI WCCGGW 3 cut(s) 152, 169, 324
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 331, 574, 650
Bse1I ACTGG 4 cut(s) 193, 432, 961, 1047
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 3 cut(s) 358, 1214, 1245
BseDI CCNNGG 1 cut(s) 649
BseGI GGATG 5 cut(s) 202, 624, 850, 985, 1123
BseLI CCNNNNNNNGG 4 cut(s) 71, 331, 574, 650
BseMII CTCAG 3 cut(s) 776, 786, 1218
BseNI ACTGG 4 cut(s) 193, 432, 961, 1047
BseRI GAGGAG 1 cut(s) 750
BseSI GKGCMC 1 cut(s) 697
BseX3I CGGCCG 1 cut(s) 34
BseXI GCAGC 2 cut(s) 101, 981
BsgI GTGCAG 2 cut(s) 100, 1040
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 3 cut(s) 36, 669, 695
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 4 cut(s) 72, 153, 170, 325
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 4 cut(s) 71, 331, 574, 650
BsmAI GTCTC 3 cut(s) 112, 273, 445
BsmBI CGTCTC 2 cut(s) 112, 445
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 3 cut(s) 36, 669, 695
Bsp119I TTCGAA 1 cut(s) 410
Bsp120I GGGCCC 1 cut(s) 693
Bsp1286I GDGCHC 2 cut(s) 636, 697
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 4 cut(s) 189, 199, 1043, 1207
BspACI CCGC 5 cut(s) 37, 57, 69, 102, 181
BspANI GGCC 3 cut(s) 36, 669, 695
BspCNI CTCAG 3 cut(s) 775, 787, 1219
BspEI TCCGGA 1 cut(s) 169
BspLI GGNNCC 5 cut(s) 191, 320, 695, 1045, 1093
BspMAI CTGCAG 1 cut(s) 98
BspPI GGATC 4 cut(s) 184, 197, 1038, 1051
BspQI GCTCTTC 1 cut(s) 836
BspT104I TTCGAA 1 cut(s) 410
BsrI ACTGG 4 cut(s) 193, 432, 961, 1047
BssECI CCNNGG 1 cut(s) 649
BssMI GATC 4 cut(s) 189, 199, 1043, 1207
Bst2UI CCWGG 3 cut(s) 358, 1214, 1245
Bst4CI ACNGT 6 cut(s) 232, 469, 510, 1004, 1096, 1150
Bst6I CTCTTC 4 cut(s) 92, 516, 836, 933
BstBAI YACGTR 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 380
BstDEI CTNAG 4 cut(s) 762, 795, 1080, 1227
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 5 cut(s) 202, 624, 850, 985, 1123
BstHHI GCGC 2 cut(s) 6, 482
BstKTI GATC 4 cut(s) 192, 202, 1046, 1210
BstMAI GTCTC 3 cut(s) 112, 273, 445
BstMBI GATC 4 cut(s) 189, 199, 1043, 1207
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 1188
BstNI CCWGG 3 cut(s) 358, 1214, 1245
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 3 cut(s) 356, 1212, 1243
BstSFI CTRYAG 3 cut(s) 94, 637, 1029
BstSLI GKGCMC 1 cut(s) 697
BstV1I GCAGC 2 cut(s) 101, 981
BstX2I RGATCY 2 cut(s) 189, 1043
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 2 cut(s) 189, 1043
BstZI CGGCCG 1 cut(s) 34
BsuI GTATCC 2 cut(s) 536, 1138
BsuRI GGCC 3 cut(s) 36, 669, 695
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 5 cut(s) 202, 624, 850, 985, 1123
BtsI GCAGTG 1 cut(s) 1210
BtsIMutI CAGTG 2 cut(s) 1054, 1210
Cac8I GCNNGC 1 cut(s) 380
CfoI GCGC 2 cut(s) 6, 482
Cfr13I GGNCC 4 cut(s) 319, 667, 693, 694
CseI GACGC 4 cut(s) 6, 20, 131, 462
Csp6I GTAC 2 cut(s) 241, 1111
CviAII CATG 5 cut(s) 111, 164, 283, 629, 808
CviQI GTAC 2 cut(s) 241, 1111
DdeI CTNAG 4 cut(s) 762, 795, 1080, 1227
DpnI GATC 4 cut(s) 191, 201, 1045, 1209
DpnII GATC 4 cut(s) 189, 199, 1043, 1207
DriI GACNNNNNGTC 2 cut(s) 16, 104
EaeI YGGCCR 1 cut(s) 34
EagI CGGCCG 1 cut(s) 34
Eam1104I CTCTTC 4 cut(s) 92, 516, 836, 933
Eam1105I GACNNNNNGTC 2 cut(s) 16, 104
EarI CTCTTC 4 cut(s) 92, 516, 836, 933
EciI GGCGGA 1 cut(s) 170
EclXI CGGCCG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 697
Eco47I GGWCC 1 cut(s) 319
Eco52I CGGCCG 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 332
Eco72I CACGTG 1 cut(s) 43
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 693
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 3 cut(s) 356, 1212, 1243
EcoT22I ATGCAT 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 697
Esp3I CGTCTC 2 cut(s) 112, 445
FaeI CATG 5 cut(s) 114, 167, 286, 632, 811
FaqI GGGAC 1 cut(s) 332
FatI CATG 5 cut(s) 110, 163, 282, 628, 807
FauI CCCGC 2 cut(s) 64, 109
FauNDI CATATG 2 cut(s) 338, 814
FbaI TGATCA 1 cut(s) 1207
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 5 cut(s) 7, 15, 37, 115, 970
FokI GGATG 5 cut(s) 189, 611, 837, 972, 1130
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 5 cut(s) 7, 15, 37, 115, 970
FspBI CTAG 3 cut(s) 209, 645, 885
GlaI GCGC 2 cut(s) 5, 481
GluI GCNGC 5 cut(s) 7, 15, 37, 115, 970
GsuI CTGGAG 1 cut(s) 1151
HaeIII GGCC 3 cut(s) 36, 669, 695
HapII CCGG 4 cut(s) 72, 153, 170, 325
HgaI GACGC 4 cut(s) 6, 20, 131, 462
HhaI GCGC 2 cut(s) 6, 482
Hin1II CATG 5 cut(s) 114, 167, 286, 632, 811
Hin6I GCGC 2 cut(s) 4, 480
HinP1I GCGC 2 cut(s) 4, 480
HincII GTYRAC 2 cut(s) 789, 1099
HindII GTYRAC 2 cut(s) 789, 1099
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 2 cut(s) 673, 870
HpaII CCGG 4 cut(s) 72, 153, 170, 325
HphI GGTGA 4 cut(s) 625, 755, 1064, 1202
Hpy166II GTNNAC 4 cut(s) 228, 391, 789, 1099
Hpy188I TCNGA 8 cut(s) 199, 312, 709, 765, 796, 1117, 1228, 1286
Hpy188III TCNNGA 7 cut(s) 170, 218, 490, 554, 603, 1142, 1168
Hpy8I GTNNAC 4 cut(s) 228, 391, 789, 1099
Hpy99I CGWCG 3 cut(s) 14, 53, 147
HpyAV CCTTC 2 cut(s) 1246, 1250
HpyCH4III ACNGT 6 cut(s) 232, 469, 510, 1004, 1096, 1150
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 9 cut(s) 96, 117, 382, 589, 953, 985, 1021, 1191, 1274
HpyF10VI GCNNNNNNNGC 1 cut(s) 1188
HpyF3I CTNAG 4 cut(s) 762, 795, 1080, 1227
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 5 cut(s) 114, 167, 286, 632, 811
HspAI GCGC 2 cut(s) 4, 480
Kpn2I TCCGGA 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 1207
Kzo9I GATC 4 cut(s) 189, 199, 1043, 1207
LguI GCTCTTC 1 cut(s) 836
LmnI GCTCC 2 cut(s) 172, 403
Lsp1109I GCAGC 2 cut(s) 101, 981
LweI GCATC 3 cut(s) 576, 994, 1283
MaeI CTAG 3 cut(s) 209, 645, 885
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 2 cut(s) 554, 620
MalI GATC 4 cut(s) 191, 201, 1045, 1209
MboI GATC 4 cut(s) 189, 199, 1043, 1207
MboII GAAGA 4 cut(s) 79, 533, 853, 950
MflI RGATCY 2 cut(s) 189, 1043
MhlI GDGCHC 2 cut(s) 636, 697
MluCI AATT 6 cut(s) 415, 502, 533, 657, 662, 961
MlyI GAGTC 1 cut(s) 879
MmeI TCCRAC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 591
MroI TCCGGA 1 cut(s) 169
MseI TTAA 2 cut(s) 660, 999
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 4 cut(s) 72, 153, 170, 325
MspR9I CCNGG 3 cut(s) 358, 1214, 1245
MvaI CCWGG 3 cut(s) 358, 1214, 1245
MwoI GCNNNNNNNGC 1 cut(s) 1188
NdeI CATATG 2 cut(s) 338, 814
NdeII GATC 4 cut(s) 189, 199, 1043, 1207
NlaIII CATG 5 cut(s) 114, 167, 286, 632, 811
NlaIV GGNNCC 5 cut(s) 191, 320, 695, 1045, 1093
NmeAIII GCCGAG 1 cut(s) 674
NmuCI GTSAC 1 cut(s) 554
NsiI ATGCAT 1 cut(s) 591
NspI RCATGY 1 cut(s) 286
NspV TTCGAA 1 cut(s) 410
PciI ACATGT 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 836
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 236
PfeI GAWTC 1 cut(s) 673
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 5 cut(s) 8, 16, 38, 116, 971
PleI GAGTC 1 cut(s) 878
PmaCI CACGTG 1 cut(s) 43
PmlI CACGTG 1 cut(s) 43
PpsI GAGTC 1 cut(s) 878
Ppu21I YACGTR 1 cut(s) 43
PscI ACATGT 1 cut(s) 282
PsiI TTATAA 1 cut(s) 447
Psp6I CCWGG 3 cut(s) 356, 1212, 1243
PspCI CACGTG 1 cut(s) 43
PspGI CCWGG 3 cut(s) 356, 1212, 1243
PspN4I GGNNCC 5 cut(s) 191, 320, 695, 1045, 1093
PspOMI GGGCCC 1 cut(s) 693
PspPI GGNCC 4 cut(s) 319, 667, 693, 694
PstI CTGCAG 1 cut(s) 98
PsuI RGATCY 2 cut(s) 189, 1043
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 2 cut(s) 242, 1112
RsaNI GTAC 2 cut(s) 241, 1111
RseI CAYNNNNRTG 1 cut(s) 629
SapI GCTCTTC 1 cut(s) 836
SaqAI TTAA 2 cut(s) 660, 999
SatI GCNGC 5 cut(s) 7, 15, 37, 115, 970
Sau3AI GATC 4 cut(s) 189, 199, 1043, 1207
Sau96I GGNCC 4 cut(s) 319, 667, 693, 694
SchI GAGTC 1 cut(s) 879
ScrFI CCNGG 3 cut(s) 358, 1214, 1245
SduI GDGCHC 2 cut(s) 636, 697
SfaNI GCATC 3 cut(s) 576, 994, 1283
SfcI CTRYAG 3 cut(s) 94, 637, 1029
SfuI TTCGAA 1 cut(s) 410
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 629
SmlI CTYRAG 3 cut(s) 137, 216, 858
SmoI CTYRAG 3 cut(s) 137, 216, 858
Sse9I AATT 6 cut(s) 415, 502, 533, 657, 662, 961
SsiI CCGC 5 cut(s) 37, 57, 69, 102, 181
SspMI CTAG 3 cut(s) 209, 645, 885
StyD4I CCNGG 3 cut(s) 356, 1212, 1243
TaaI ACNGT 6 cut(s) 232, 469, 510, 1004, 1096, 1150
TaiI ACGT 1 cut(s) 45
TaqI TCGA 2 cut(s) 145, 410
TasI AATT 6 cut(s) 415, 502, 533, 657, 662, 961
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 2 cut(s) 660, 999
Tru9I TTAA 2 cut(s) 660, 999
TscAI CASTG 2 cut(s) 1054, 1210
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 4 cut(s) 6, 14, 114, 969
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 3 cut(s) 360, 534, 951
TspGWI ACGGA 2 cut(s) 37, 561
TspRI CASTG 2 cut(s) 1054, 1210
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 319
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 2 cut(s) 533, 961
XceI RCATGY 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 3 cut(s) 209, 645, 885
Zsp2I ATGCAT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.