Rh7BG069300

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012263
Physical Location & Seq
Reverse (-)
4608031 .. 4611879
3849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG069300.1

Sequence Viewer

Length: 1113 bp
ATGCGCTGCGACGCTGCTTCCTCTTGGCTCTGCGGCCGCCACGTCACCGTCGCAACCGCCATGCCGAACGCCGGAACCTCCTTCTTCTTCCTCTGCAGACCCACCGTCTCCTGCTGCACCAACACTCCCAACCCACTCAAGCGGCGATACTCCAGTAGTAGCCATGCTCCGGACGTTGTCCGCCGTTGGATTCAATCCGATCATCCGCTAGCTTCTCAGGATAGGTTCACGGTTGCTTCGTACAATATTTTGGGGGATAGAAATGCTTTTCGGCATGGAGACCTGTACGGGAATGTGCCTTCTATGTATCTGAAATGGGACCGCCGGAAAAGGGTCATCTGCGATGAACTTGCTGCCTGGAATTCTGATATAGTTTGCTTGCAAGAGGTGGACAAGTATTTGGAGCTCGCGGATATTTTGGCAAAACTGGGATATGCTGGTTCTTATAAGAGACGCACAGGAGATACCGTTGACGGTTGCGCTACTTTCTGGAAATCTCATAATTTTCGGTTGTTAGAGGAAGAGAGCATTGAATTCAAGGGATATGGACTTCGTGACAATGTTGCTCAACTCTCGGTTTTTGAGATGCATAAAGACAAATCAAGAAAGTTAGTGATCGGTAACATCCATGTGCTTTACAGGCCAAGCCGAGGAGAAGTTAAATTGGGTCAGATTCGTTTTCTCATATCAAGGGCGCAGATCCTCTCGGAGAAATGGGGTAATGCCCCTGTTGTCCTTTGTGGTGATTTCAATAGCACTCCTCAGAGTGCAATATACAAGTTCTTGTCAACATCTGAGCTAAATATCATGTCATATGACAGAACAGAGTTATCTGGGCAGAGGAGCTGTCATCCTGCTCAAGTCTTAGGTGTCAAACAACGAATTAGCAGTCCATTGAGCTTGATAGATGGATTGTTGAAACATAACTGGACAGATGAAGAGGTTAGAGTTGCAACTGGAAATTCTGGCAGCCATTCAGTTGTGCATCCCTTGAAGCTTAACAGTGCTTATGCTACGGTTCAGGGTTCTACAGCAACACGGGGATCCAGTGGTGAGCCTTTAGCTACTTCTTACCACTCAAAGTTTCTTGGAACCGTTGACTACTTATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

370

Amino Acids

41.45

Weight (kDa)

9.09

Isoelectric Point (pI)

53.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 79 - 274 3.7e-16 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18500 AT3G18500 AT3G18500 AT3G18500
fragaria_vesca FvH4_5g12590
malus_domestica MD06G1212300.v1.1
prunus_persica Prupe.5G218400_v2.0.a1
pyrus_communis pycom06g18950
rosa_chinensis RchiOBHm_Chr7g0184741
rosa_laevigata RLG00000004954
rosa_multiflora Rmu_co8486587.1_g000001 Rmu_ssc0000289.1_g000017
rosa_roxburghii Rroxscaffold_3G00269380
rosa_rugosa Rorug06G0464900
rosa_samantha Rh7AG078000 Rh7BG069300 Rh7CG070100 Rh7DG069500
rosa_wichuraiana Rw7G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccII CGCG 1 cut(s) 410
AccIII TCCGGA 1 cut(s) 169
AciI CCGC 8 cut(s) 33, 37, 57, 142, 181, 206, 322, 410
AclWI GGATC 3 cut(s) 694, 1038, 1051
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 3 cut(s) 361, 533, 961
AfaI GTAC 2 cut(s) 242, 287
AfiI CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
AgsI TTSAA 6 cut(s) 194, 533, 538, 751, 919, 994
AhdI GACNNNNNGTC 1 cut(s) 104
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 7 cut(s) 212, 406, 799, 846, 900, 997, 1064
AluI AGCT 7 cut(s) 212, 406, 799, 846, 900, 997, 1064
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 3 cut(s) 112, 273, 445
AlwI GGATC 3 cut(s) 694, 1038, 1051
Aor13HI TCCGGA 1 cut(s) 169
AoxI GGCC 2 cut(s) 34, 641
ApeKI GCWGC 5 cut(s) 6, 14, 114, 353, 969
ApoI RAATTY 3 cut(s) 361, 533, 961
ArsI GACNNNNNNTTYG 2 cut(s) 874, 906
AspLEI GCGC 3 cut(s) 6, 482, 697
AspS9I GGNCC 1 cut(s) 319
AsuHPI GGTGA 3 cut(s) 37, 755, 1064
AsuNHI GCTAGC 1 cut(s) 208
AvaII GGWCC 1 cut(s) 319
BaeI ACNNNNGTAYC 2 cut(s) 139, 172
BamHI GGATCC 1 cut(s) 1043
BanII GRGCYC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 101, 340, 981
BccI CCATC 1 cut(s) 902
BceAI ACGGC 1 cut(s) 168
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 1 cut(s) 358
BcoDI GTCTC 3 cut(s) 112, 273, 445
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 2 cut(s) 94, 1029
BisI GCNGC 8 cut(s) 7, 15, 34, 37, 115, 143, 354, 970
BlsI GCNGC 8 cut(s) 8, 16, 35, 38, 116, 144, 355, 971
Bme1390I CCNGG 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 319
BmeRI GACNNNNNGTC 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 4 cut(s) 76, 320, 1045, 1093
BmrFI CCNGG 1 cut(s) 358
BmrI ACTGGG 1 cut(s) 437
BmsI GCATC 2 cut(s) 576, 994
BmtI GCTAGC 1 cut(s) 212
BmuI ACTGGG 1 cut(s) 437
BpmI CTGGAG 1 cut(s) 136
BpuEI CTTGAG 2 cut(s) 122, 843
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 649
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
Bse1I ACTGG 5 cut(s) 153, 432, 932, 961, 1047
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 1 cut(s) 649
BseGI GGATG 4 cut(s) 202, 624, 850, 985
BseLI CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
BseMII CTCAG 3 cut(s) 230, 776, 786
BseNI ACTGG 5 cut(s) 153, 432, 932, 961, 1047
BseRI GAGGAG 3 cut(s) 666, 750, 856
BseX3I CGGCCG 1 cut(s) 34
BseXI GCAGC 3 cut(s) 101, 340, 981
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 410
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 36, 643
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 3 cut(s) 72, 170, 325
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
BsmAI GTCTC 3 cut(s) 112, 273, 445
BsmBI CGTCTC 2 cut(s) 112, 445
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 2 cut(s) 36, 643
Bso31I GGTCTC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 408
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 4 cut(s) 199, 615, 699, 1043
BspACI CCGC 8 cut(s) 33, 37, 57, 142, 181, 206, 322, 410
BspANI GGCC 2 cut(s) 36, 643
BspCNI CTCAG 3 cut(s) 229, 775, 787
BspEI TCCGGA 1 cut(s) 169
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 4 cut(s) 76, 320, 1045, 1093
BspMAI CTGCAG 1 cut(s) 98
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 3 cut(s) 694, 1038, 1051
BspTNI GGTCTC 1 cut(s) 273
BsrI ACTGG 5 cut(s) 153, 432, 932, 961, 1047
BssECI CCNNGG 1 cut(s) 649
BssMI GATC 4 cut(s) 199, 615, 699, 1043
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 8 cut(s) 49, 106, 232, 469, 476, 1004, 1018, 1096
Bst6I CTCTTC 2 cut(s) 516, 933
BstC8I GCNNGC 3 cut(s) 210, 380, 408
BstDEI CTNAG 4 cut(s) 216, 762, 795, 865
BstF5I GGATG 4 cut(s) 202, 624, 850, 985
BstFNI CGCG 1 cut(s) 410
BstHHI GCGC 3 cut(s) 6, 482, 697
BstKTI GATC 4 cut(s) 202, 618, 702, 1046
BstMAI GTCTC 3 cut(s) 112, 273, 445
BstMBI GATC 4 cut(s) 199, 615, 699, 1043
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 640
BstNI CCWGG 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 356
BstSFI CTRYAG 2 cut(s) 94, 1029
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 3 cut(s) 101, 340, 981
BstX2I RGATCY 2 cut(s) 699, 1043
BstYI RGATCY 2 cut(s) 699, 1043
BstZI CGGCCG 1 cut(s) 34
BsuRI GGCC 2 cut(s) 36, 643
BtgZI GCGATG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 4 cut(s) 202, 624, 850, 985
BtsIMutI CAGTG 2 cut(s) 1009, 1054
Cac8I GCNNGC 3 cut(s) 210, 380, 408
CciNI GCGGCCGC 1 cut(s) 34
CfoI GCGC 3 cut(s) 6, 482, 697
Cfr13I GGNCC 1 cut(s) 319
CseI GACGC 2 cut(s) 20, 462
Csp6I GTAC 2 cut(s) 241, 286
CviAII CATG 5 cut(s) 61, 164, 275, 629, 808
CviQI GTAC 2 cut(s) 241, 286
DdeI CTNAG 4 cut(s) 216, 762, 795, 865
DpnI GATC 4 cut(s) 201, 617, 701, 1045
DpnII GATC 4 cut(s) 199, 615, 699, 1043
DriI GACNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 34
EagI CGGCCG 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 516, 933
Eam1105I GACNNNNNGTC 1 cut(s) 104
EarI CTCTTC 2 cut(s) 516, 933
EciI GGCGGA 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 406
EclXI CGGCCG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 319
Eco52I CGGCCG 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 406
EcoRI GAATTC 2 cut(s) 361, 533
EcoRII CCWGG 1 cut(s) 356
EcoT22I ATGCAT 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 408
Esp3I CGTCTC 2 cut(s) 112, 445
FaeI CATG 5 cut(s) 64, 167, 278, 632, 811
FaqI GGGAC 1 cut(s) 332
FatI CATG 5 cut(s) 60, 163, 274, 628, 807
FauNDI CATATG 1 cut(s) 814
Fnu4HI GCNGC 8 cut(s) 7, 15, 34, 37, 115, 143, 354, 970
FokI GGATG 4 cut(s) 189, 611, 837, 972
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 8 cut(s) 7, 15, 34, 37, 115, 143, 354, 970
FspBI CTAG 1 cut(s) 209
GlaI GCGC 3 cut(s) 5, 481, 696
GluI GCNGC 8 cut(s) 7, 15, 34, 37, 115, 143, 354, 970
GsuI CTGGAG 1 cut(s) 136
HaeIII GGCC 2 cut(s) 36, 643
HapII CCGG 3 cut(s) 72, 170, 325
HgaI GACGC 2 cut(s) 20, 462
HhaI GCGC 3 cut(s) 6, 482, 697
Hin1II CATG 5 cut(s) 64, 167, 278, 632, 811
Hin6I GCGC 3 cut(s) 4, 480, 695
HinP1I GCGC 3 cut(s) 4, 480, 695
HincII GTYRAC 3 cut(s) 472, 789, 1099
HindII GTYRAC 3 cut(s) 472, 789, 1099
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 2 cut(s) 190, 673
HpaII CCGG 3 cut(s) 72, 170, 325
HphI GGTGA 3 cut(s) 37, 755, 1064
Hpy166II GTNNAC 5 cut(s) 228, 391, 472, 789, 1099
Hpy188I TCNGA 7 cut(s) 199, 312, 367, 672, 709, 765, 796
Hpy188III TCNNGA 5 cut(s) 170, 218, 490, 554, 603
Hpy8I GTNNAC 5 cut(s) 228, 391, 472, 789, 1099
Hpy99I CGWCG 2 cut(s) 14, 53
HpyAV CCTTC 2 cut(s) 91, 309
HpyCH4III ACNGT 8 cut(s) 49, 106, 232, 469, 476, 1004, 1018, 1096
HpyCH4IV ACGT 2 cut(s) 42, 174
HpyCH4V TGCA 7 cut(s) 96, 117, 382, 589, 770, 953, 985
HpyF10VI GCNNNNNNNGC 1 cut(s) 640
HpyF3I CTNAG 4 cut(s) 216, 762, 795, 865
HpySE526I ACGT 2 cut(s) 42, 174
Hsp92II CATG 5 cut(s) 64, 167, 278, 632, 811
HspAI GCGC 3 cut(s) 4, 480, 695
Kpn2I TCCGGA 1 cut(s) 169
Kzo9I GATC 4 cut(s) 199, 615, 699, 1043
LmnI GCTCC 3 cut(s) 172, 403, 843
Lsp1109I GCAGC 3 cut(s) 101, 340, 981
LweI GCATC 2 cut(s) 576, 994
MaeI CTAG 1 cut(s) 209
MaeII ACGT 2 cut(s) 42, 174
MaeIII GTNAC 3 cut(s) 43, 554, 620
MalI GATC 4 cut(s) 201, 617, 701, 1045
MboI GATC 4 cut(s) 199, 615, 699, 1043
MboII GAAGA 4 cut(s) 76, 79, 533, 950
MflI RGATCY 2 cut(s) 699, 1043
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 6 cut(s) 361, 502, 533, 662, 882, 961
MmeI TCCRAC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 591
MroI TCCGGA 1 cut(s) 169
MseI TTAA 2 cut(s) 660, 999
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 3 cut(s) 72, 170, 325
MspR9I CCNGG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 1 cut(s) 640
NdeI CATATG 1 cut(s) 814
NdeII GATC 4 cut(s) 199, 615, 699, 1043
NheI GCTAGC 1 cut(s) 208
NlaIII CATG 5 cut(s) 64, 167, 278, 632, 811
NlaIV GGNNCC 4 cut(s) 76, 320, 1045, 1093
NmeAIII GCCGAG 1 cut(s) 674
NmuCI GTSAC 2 cut(s) 43, 554
NotI GCGGCCGC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PfeI GAWTC 2 cut(s) 190, 673
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 8 cut(s) 8, 16, 35, 38, 116, 144, 355, 971
PsiI TTATAA 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 4 cut(s) 76, 320, 1045, 1093
PspPI GGNCC 1 cut(s) 319
PstI CTGCAG 1 cut(s) 98
PsuI RGATCY 2 cut(s) 699, 1043
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 2 cut(s) 242, 287
RsaNI GTAC 2 cut(s) 241, 286
RseI CAYNNNNRTG 1 cut(s) 629
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 2 cut(s) 660, 999
SatI GCNGC 8 cut(s) 7, 15, 34, 37, 115, 143, 354, 970
Sau3AI GATC 4 cut(s) 199, 615, 699, 1043
Sau96I GGNCC 1 cut(s) 319
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 1 cut(s) 408
SfaNI GCATC 2 cut(s) 576, 994
SfcI CTRYAG 2 cut(s) 94, 1029
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 629
SmlI CTYRAG 2 cut(s) 137, 858
SmoI CTYRAG 2 cut(s) 137, 858
Sse9I AATT 6 cut(s) 361, 502, 533, 662, 882, 961
SsiI CCGC 8 cut(s) 33, 37, 57, 142, 181, 206, 322, 410
SspI AATATT 1 cut(s) 247
SspMI CTAG 1 cut(s) 209
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 356
TaaI ACNGT 8 cut(s) 49, 106, 232, 469, 476, 1004, 1018, 1096
TaiI ACGT 2 cut(s) 45, 177
TasI AATT 6 cut(s) 361, 502, 533, 662, 882, 961
TauI GCSGC 3 cut(s) 36, 39, 145
TfiI GAWTC 2 cut(s) 190, 673
Tru1I TTAA 2 cut(s) 660, 999
Tru9I TTAA 2 cut(s) 660, 999
TscAI CASTG 2 cut(s) 1009, 1054
TseFI GTSAC 2 cut(s) 43, 554
TseI GCWGC 5 cut(s) 6, 14, 114, 353, 969
Tsp45I GTSAC 2 cut(s) 43, 554
TspDTI ATGAA 2 cut(s) 360, 951
TspRI CASTG 2 cut(s) 1009, 1054
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 3 cut(s) 361, 533, 961
XspI CTAG 1 cut(s) 209
Zsp2I ATGCAT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.