Prupe.5G218400_v2.0.a1

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012263
Physical Location & Seq
Reverse (-)
17088777 .. 17091892
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G218400.1

Sequence Viewer

Length: 1341 bp
ATGCGGTGCGACGCTTCCTCTTGGCTCTGCGGGCGCCACGTATCCGTAACAACAGCCGCACCGACGAGAAGCGCCACCGGCCCATCCTCCTTCTTCCTCTGCAGCAAGCCCACCGTCTTTTGCTGCACCGACGCTTCCAAAGACTCATCATCTACATGTTCCGACACAGCTCGTCCATTGTACAACCCCCTCAAGCGGCGACAGTCAAGTCATGCTCCGGATGTTGTACGCCATTGGATCCAAACCGATAATCAACAACCACTGTCTTCTCAAGATAGATTTACGGTTGCTTCGTATAACATTTTGGGGGATAGAAATGCTTTCGCGCATAGAGATATGTACCGCAACGTGCCTTCCCATTATCTCAAATGGGACCGTCGTAAGAGGGTCATATGCGATGAACTTGTCCAATGGAACCCTGATATAATCTGTTTGCAAGAAGTAGATAAGTATTTTGAGCTATCAGAAATTCTAGCGAAAGTGGGATATCTTGGGTCGTATAAGAGACGCACGGGAGATACCGTTGATGGTTGCGCTATTTTCTGGAAGGCTGATAATTTTCAGTTGTTAGAGGAACACAGCATTGAATTCAAGGGTTATGGACTTCGTGACAATGTTGCTCAACTCTCGGTTTTTGAGATGCAGAAAGCCGAATCAAGAAGGTTCGTGATTGGTAACATACACGTGCTTTATAACCCAAGCCGAGGGGAGGTAAAATTGGGCCAGATACGTTTTCTCATATCAAGGGCACAAATCCTCTCAGAGAGATGGGGCAATGCCCCCATTGTCCTTTGTGGTGATTTCAATAGCACTCCGCAGAGTGCAATATACAAGTTCTTGTCAACATCTGAGCTGAATATCATGTTATATGACAGAAGAGAGTTATCTGGGCAGAGAGACTGTCATCCTGCTCAAGTTTTAGGAGTCAAACAAGAAATTAGCAGTCCACTTACACTGATAGACGGATTGTTGAAGCATTGCTGGACAGATGAAGAGGTTAGAGTTGCAACTGGAGATGCTGAGAGCAATTTAGTTGTGCATCCCCTGAAGCTTAACAGTTCCTATGCCACAGTGCGGGGTACGACTAGAACACGAGGATCCAACGGCGAACCGTTAGCGACTTCCTACCACTCAAAATTTCTTGGAACTGTTGACTACTTATGGTACTCTGATGGCCTTGTGCCCACTGGAGTTATAGATACCGTTCCAGTTGACATTCTCCAGAGGATTGGTAGCCTCCCTTGCAAGAAAGTGGGCAGCGACCACTTGGCCTTAGTTTCTGAGTTTGCCTTCACACTGGACACAAATGGAGACAACAAAACAGCTGCCTCAAGTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

447

Amino Acids

49.98

Weight (kDa)

7.91

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18500 AT3G18500 AT3G18500 AT3G18500
fragaria_vesca FvH4_5g12590
malus_domestica MD06G1212300.v1.1
prunus_persica Prupe.5G218400_v2.0.a1
pyrus_communis pycom06g18950
rosa_chinensis RchiOBHm_Chr7g0184741
rosa_laevigata RLG00000004954
rosa_multiflora Rmu_co8486587.1_g000001 Rmu_ssc0000289.1_g000017
rosa_roxburghii Rroxscaffold_3G00269380
rosa_rugosa Rorug06G0464900
rosa_samantha Rh7AG078000 Rh7BG069300 Rh7CG070100 Rh7DG069500
rosa_wichuraiana Rw7G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AasI GACNNNNNNGTC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 33
AccII CGCG 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 7 cut(s) 4, 30, 57, 196, 343, 815, 1075
AclWI GGATC 4 cut(s) 232, 245, 1092, 1105
AcsI RAATTY 3 cut(s) 468, 587, 1136
AcuI CTGAAG 1 cut(s) 1067
AcvI CACGTG 1 cut(s) 685
AcyI GRCGYC 1 cut(s) 34
AfaI GTAC 5 cut(s) 182, 228, 341, 1081, 1166
AfiI CCNNNNNNNGG 4 cut(s) 195, 704, 709, 1074
AflIII ACRYGT 2 cut(s) 155, 682
AgsI TTSAA 4 cut(s) 587, 592, 805, 973
AluBI AGCT 5 cut(s) 170, 460, 853, 1051, 1325
AluI AGCT 5 cut(s) 170, 460, 853, 1051, 1325
Alw26I GTCTC 3 cut(s) 499, 891, 1305
AlwI GGATC 4 cut(s) 232, 245, 1092, 1105
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 4 cut(s) 79, 721, 1174, 1269
ApeKI GCWGC 4 cut(s) 102, 123, 1257, 1325
ApoI RAATTY 3 cut(s) 468, 587, 1136
AspLEI GCGC 4 cut(s) 36, 74, 328, 536
AspS9I GGNCC 3 cut(s) 80, 373, 721
AsuHPI GGTGA 1 cut(s) 809
AvaII GGWCC 1 cut(s) 373
BaeGI GKGCMC 2 cut(s) 751, 1185
BamHI GGATCC 2 cut(s) 237, 1097
BanI GGYRCC 1 cut(s) 33
BauI CACGAG 1 cut(s) 1092
BbrPI CACGTG 1 cut(s) 685
BbsI GAAGAC 1 cut(s) 258
BbvI GCAGC 4 cut(s) 110, 114, 1269, 1312
BccI CCATC 4 cut(s) 91, 521, 762, 1166
BceAI ACGGC 1 cut(s) 1120
BciVI GTATCC 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 499, 891, 1305
BfaI CTAG 2 cut(s) 473, 1086
BfmI CTRYAG 1 cut(s) 100
BfoI RGCGCY 2 cut(s) 37, 75
BfuI GTATCC 1 cut(s) 52
BisI GCNGC 6 cut(s) 57, 103, 124, 197, 1258, 1326
BlsI GCNGC 6 cut(s) 58, 104, 125, 198, 1259, 1327
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 3 cut(s) 80, 373, 721
BmiI GGNNCC 5 cut(s) 35, 239, 374, 416, 1099
BmsI GCATC 3 cut(s) 630, 1006, 1048
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 3 cut(s) 1032, 1205, 1209
BpuEI CTTGAG 4 cut(s) 176, 255, 897, 1315
BsaAI YACGTR 2 cut(s) 40, 685
BsaHI GRCGYC 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 703
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 704, 709, 1074
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 4 cut(s) 1015, 1192, 1208, 1302
Bse3DI GCAATG 2 cut(s) 781, 976
BseAI TCCGGA 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 703
BseGI GGATG 4 cut(s) 83, 226, 904, 1039
BseLI CCNNNNNNNGG 4 cut(s) 195, 704, 709, 1074
BseMI GCAATG 2 cut(s) 781, 976
BseMII CTCAG 4 cut(s) 774, 840, 1011, 1272
BseNI ACTGG 4 cut(s) 1015, 1192, 1208, 1302
BseSI GKGCMC 2 cut(s) 751, 1185
BseXI GCAGC 4 cut(s) 110, 114, 1269, 1312
BsgI GTGCAG 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 4 cut(s) 81, 723, 1176, 1271
BshNI GGYRCC 1 cut(s) 33
BsiSI CCGG 2 cut(s) 78, 218
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 4 cut(s) 195, 704, 709, 1074
BsmAI GTCTC 3 cut(s) 499, 891, 1305
BsmBI CGTCTC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 386
BsnI GGCC 4 cut(s) 81, 723, 1176, 1271
Bsp1286I GDGCHC 2 cut(s) 751, 1185
Bsp13I TCCGGA 1 cut(s) 217
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 2 cut(s) 237, 1097
BspACI CCGC 7 cut(s) 4, 30, 57, 196, 343, 815, 1075
BspANI GGCC 4 cut(s) 81, 723, 1176, 1271
BspCNI CTCAG 4 cut(s) 773, 841, 1012, 1273
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 5 cut(s) 35, 239, 374, 416, 1099
BspMAI CTGCAG 1 cut(s) 104
BspPI GGATC 4 cut(s) 232, 245, 1092, 1105
BspT107I GGYRCC 1 cut(s) 33
BsrDI GCAATG 2 cut(s) 781, 976
BsrFI RCCGGY 1 cut(s) 77
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 4 cut(s) 1015, 1192, 1208, 1302
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 703
BssMI GATC 2 cut(s) 237, 1097
BssNI GRCGYC 1 cut(s) 34
BssSI CACGAG 1 cut(s) 1092
Bst2BI CACGAG 1 cut(s) 1092
Bst6I CTCTTC 2 cut(s) 871, 987
BstACI GRCGYC 1 cut(s) 34
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 2 cut(s) 40, 685
BstC8I GCNNGC 2 cut(s) 32, 107
BstDEI CTNAG 5 cut(s) 760, 849, 1020, 1273, 1281
BstF5I GGATG 4 cut(s) 83, 226, 904, 1039
BstFNI CGCG 1 cut(s) 326
BstH2I RGCGCY 2 cut(s) 37, 75
BstHHI GCGC 4 cut(s) 36, 74, 328, 536
BstKTI GATC 2 cut(s) 240, 1100
BstMAI GTCTC 3 cut(s) 499, 891, 1305
BstMBI GATC 2 cut(s) 237, 1097
BstMWI GCNNNNNNNGC 3 cut(s) 31, 78, 1242
BstNSI RCATGY 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 100
BstSLI GKGCMC 2 cut(s) 751, 1185
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 4 cut(s) 110, 114, 1269, 1312
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 2 cut(s) 237, 1097
BstXI CCANNNNNNTGG 1 cut(s) 1229
BstYI RGATCY 2 cut(s) 237, 1097
BsuI GTATCC 1 cut(s) 52
BsuRI GGCC 4 cut(s) 81, 723, 1176, 1271
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 4 cut(s) 83, 226, 904, 1039
BtsIMutI CAGTG 5 cut(s) 260, 953, 1077, 1185, 1295
Cac8I GCNNGC 2 cut(s) 32, 107
CfoI GCGC 4 cut(s) 36, 74, 328, 536
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 3 cut(s) 80, 373, 721
CseI GACGC 3 cut(s) 20, 140, 516
Csp6I GTAC 5 cut(s) 181, 227, 340, 1080, 1165
CviAII CATG 3 cut(s) 156, 212, 862
CviQI GTAC 5 cut(s) 181, 227, 340, 1080, 1165
DdeI CTNAG 5 cut(s) 760, 849, 1020, 1273, 1281
DinI GGCGCC 1 cut(s) 35
DpnI GATC 2 cut(s) 239, 1099
DpnII GATC 2 cut(s) 237, 1097
DrdI GACNNNNNNGTC 1 cut(s) 207
DseDI GACNNNNNNGTC 1 cut(s) 207
Eam1104I CTCTTC 2 cut(s) 871, 987
EarI CTCTTC 2 cut(s) 871, 987
Eco32I GATATC 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 1067
Eco72I CACGTG 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 587
EcoRV GATATC 1 cut(s) 488
EgeI GGCGCC 1 cut(s) 35
EheI GGCGCC 1 cut(s) 35
Esp3I CGTCTC 1 cut(s) 499
FaeI CATG 3 cut(s) 159, 215, 865
FaqI GGGAC 1 cut(s) 386
FatI CATG 3 cut(s) 155, 211, 861
FauI CCCGC 2 cut(s) 23, 1068
FauNDI CATATG 1 cut(s) 392
Fnu4HI GCNGC 6 cut(s) 57, 103, 124, 197, 1258, 1326
FokI GGATG 4 cut(s) 70, 233, 891, 1026
Fsp4HI GCNGC 6 cut(s) 57, 103, 124, 197, 1258, 1326
FspBI CTAG 2 cut(s) 473, 1086
GlaI GCGC 4 cut(s) 35, 73, 327, 535
GluI GCNGC 6 cut(s) 57, 103, 124, 197, 1258, 1326
GsuI CTGGAG 3 cut(s) 1032, 1205, 1209
HaeII RGCGCY 2 cut(s) 37, 75
HaeIII GGCC 4 cut(s) 81, 723, 1176, 1271
HapII CCGG 2 cut(s) 78, 218
HgaI GACGC 3 cut(s) 20, 140, 516
HhaI GCGC 4 cut(s) 36, 74, 328, 536
Hin1I GRCGYC 1 cut(s) 34
Hin1II CATG 3 cut(s) 159, 215, 865
Hin6I GCGC 4 cut(s) 34, 72, 326, 534
HinP1I GCGC 4 cut(s) 34, 72, 326, 534
HincII GTYRAC 3 cut(s) 843, 1153, 1213
HindII GTYRAC 3 cut(s) 843, 1153, 1213
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 3 cut(s) 143, 653, 924
HpaII CCGG 2 cut(s) 78, 218
HphI GGTGA 1 cut(s) 809
Hpy166II GTNNAC 4 cut(s) 843, 947, 1153, 1213
Hpy188I TCNGA 7 cut(s) 163, 466, 763, 850, 1171, 1282, 1340
Hpy188III TCNNGA 7 cut(s) 218, 272, 544, 608, 657, 667, 1222
Hpy8I GTNNAC 4 cut(s) 843, 947, 1153, 1213
Hpy99I CGWCG 4 cut(s) 14, 67, 134, 381
HpyAV CCTTC 5 cut(s) 100, 363, 541, 654, 1300
HpyCH4IV ACGT 4 cut(s) 39, 348, 684, 730
HpyCH4V TGCA 8 cut(s) 102, 126, 436, 643, 824, 1007, 1039, 1245
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 78, 1242
HpyF3I CTNAG 5 cut(s) 760, 849, 1020, 1273, 1281
HpySE526I ACGT 4 cut(s) 39, 348, 684, 730
Hsp92I GRCGYC 1 cut(s) 34
Hsp92II CATG 3 cut(s) 159, 215, 865
HspAI GCGC 4 cut(s) 34, 72, 326, 534
KasI GGCGCC 1 cut(s) 33
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 2 cut(s) 237, 1097
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 4 cut(s) 110, 114, 1269, 1312
LweI GCATC 3 cut(s) 630, 1006, 1048
MaeI CTAG 2 cut(s) 473, 1086
MaeII ACGT 4 cut(s) 39, 348, 684, 730
MaeIII GTNAC 3 cut(s) 46, 608, 674
MalI GATC 2 cut(s) 239, 1099
MboI GATC 2 cut(s) 237, 1097
MboII GAAGA 4 cut(s) 85, 258, 888, 1004
MflI RGATCY 2 cut(s) 237, 1097
MhlI GDGCHC 2 cut(s) 751, 1185
MluCI AATT 7 cut(s) 468, 556, 587, 716, 936, 1027, 1136
Mly113I GGCGCC 1 cut(s) 34
MlyI GAGTC 2 cut(s) 137, 933
MmeI TCCRAC 2 cut(s) 186, 1125
MroI TCCGGA 1 cut(s) 217
MseI TTAA 1 cut(s) 1053
MslI CAYNNNNRTG 2 cut(s) 154, 683
MspA1I CMGCKG 1 cut(s) 1325
MspI CCGG 2 cut(s) 78, 218
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 3 cut(s) 31, 78, 1242
NarI GGCGCC 1 cut(s) 34
NdeI CATATG 1 cut(s) 392
NdeII GATC 2 cut(s) 237, 1097
NlaIII CATG 3 cut(s) 159, 215, 865
NlaIV GGNNCC 5 cut(s) 35, 239, 374, 416, 1099
NmeAIII GCCGAG 1 cut(s) 728
NmuCI GTSAC 1 cut(s) 608
NspI RCATGY 1 cut(s) 159
PciI ACATGT 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 290
PfeI GAWTC 1 cut(s) 653
PkrI GCNGC 6 cut(s) 58, 104, 125, 198, 1259, 1327
PleI GAGTC 2 cut(s) 137, 932
PluTI GGCGCC 1 cut(s) 37
PmaCI CACGTG 1 cut(s) 685
PmlI CACGTG 1 cut(s) 685
PpsI GAGTC 2 cut(s) 137, 932
Ppu21I YACGTR 2 cut(s) 40, 685
PscI ACATGT 1 cut(s) 155
PsiI TTATAA 1 cut(s) 693
PspCI CACGTG 1 cut(s) 685
PspN4I GGNNCC 5 cut(s) 35, 239, 374, 416, 1099
PspPI GGNCC 3 cut(s) 80, 373, 721
PstI CTGCAG 1 cut(s) 104
PsuI RGATCY 2 cut(s) 237, 1097
PvuII CAGCTG 1 cut(s) 1325
RsaI GTAC 5 cut(s) 182, 228, 341, 1081, 1166
RsaNI GTAC 5 cut(s) 181, 227, 340, 1080, 1165
RseI CAYNNNNRTG 2 cut(s) 154, 683
SaqAI TTAA 1 cut(s) 1053
SatI GCNGC 6 cut(s) 57, 103, 124, 197, 1258, 1326
Sau3AI GATC 2 cut(s) 237, 1097
Sau96I GGNCC 3 cut(s) 80, 373, 721
SchI GAGTC 2 cut(s) 137, 933
SduI GDGCHC 2 cut(s) 751, 1185
SfaNI GCATC 3 cut(s) 630, 1006, 1048
SfcI CTRYAG 1 cut(s) 100
SfoI GGCGCC 1 cut(s) 35
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 154, 683
SmlI CTYRAG 4 cut(s) 191, 270, 912, 1330
SmoI CTYRAG 4 cut(s) 191, 270, 912, 1330
Sse9I AATT 7 cut(s) 468, 556, 587, 716, 936, 1027, 1136
SsiI CCGC 7 cut(s) 4, 30, 57, 196, 343, 815, 1075
SspDI GGCGCC 1 cut(s) 33
SspMI CTAG 2 cut(s) 473, 1086
TaiI ACGT 4 cut(s) 42, 351, 687, 733
TasI AATT 7 cut(s) 468, 556, 587, 716, 936, 1027, 1136
TatI WGTACW 1 cut(s) 180
TauI GCSGC 2 cut(s) 59, 199
TfiI GAWTC 1 cut(s) 653
Tru1I TTAA 1 cut(s) 1053
Tru9I TTAA 1 cut(s) 1053
TscAI CASTG 5 cut(s) 267, 960, 1077, 1192, 1302
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 4 cut(s) 102, 123, 1257, 1325
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 2 cut(s) 414, 1005
TspGWI ACGGA 2 cut(s) 34, 978
TspRI CASTG 5 cut(s) 267, 960, 1077, 1192, 1302
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 3 cut(s) 468, 587, 1136
XceI RCATGY 1 cut(s) 159
XspI CTAG 2 cut(s) 473, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.