FvH4_5g12620

pre-rRNA-processing protein TSR1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010916
Physical Location & Seq
Reverse (-)
7122401 .. 7128898
6498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12620.t2

Sequence Viewer

Length: 2373 bp
ATGGGAGGTTCGCGAAACCAAGTGAACAAGGCTCACAAGACGCGCTTCTCGTCCAAGTCGTCGCGGAATCTGCACAAAACTTCAGACAAGGGTAGGATCGGTAAATCGGAGCACAATGTTGCAAAGGGAGCTAGAGCGGCTCGAATTCAAAGGAATAAGATGATACGGGAGCAGAAGCGGGCAGCTCTTTTGAAGGAGAAGAGAGCTTCGAGTGGATCGGCAAGTCCTCCTCGTGTGATTGTTCTGTTTGGGCTCTCCGCGGATGTGAAGCTAAATTCGCTCTCCGAGGATATTCTTTCTTTGCTGTCTCCACAAGGTTATGGAGGTGCTTTGCCTACTGTAGCTTCTTCGGAGTACAGGCTGAGAGCAACGGTACTGCAAGCACCTCATGGAGATTTGCAATCATGTCTGGAAATGGCTAAGGTTGCGGATCTCATTGCCTTTGTGGCTTCAGCAACTTCTTCATGTGAAGGAGAGTCTGACTACATAGATTCATTTGGTCATCAATGTCTTTCTGTGTTTAGATCTCTTGGTTTACCGAGCACCGTCGTGCTAATACGTGATTTACCTACCGAGGCGAAAAGAAGACATGAGTCAAAGAAAACATGTAGTTCTAGTCTTGCTTCTGAATTTCCTGAAGATTGTAAATTTTATCCAGCTGATACAAAGGACGAGTTGCACAAGTTCATGTGGCTTTTTAAGGAGCAAAGGCTCTCAGTTCCTCACTGGCGAAGTCAGCGTCCTTTCCTGATGGCTCAAAAGGTTGAAATGGTAGCAGATGATATGAATCCGGGAACAAGTACCCTTCTCCTCACTGGTTATGTGCGAGCACGCAGTCTATCTGTAAATCAACTGGTTCATGTTTCTGGTGCCGGGGATTTCCAGTTGTGCAAAATGGAAATTCTCAAGGATCCACATCCTTTGAATGCAAGAAAAGAGCAGGATGTCATGGATGCAGAAGAAGTGGATGATGTAGTGGTTCGTTCTGTGGTTCCCGATCCGCTGAAGCAGGAGGCTTTACTTGTTGAGAATGTTCCTGATTGCCTTGATAATGAACAGACATGGCCGACAGAAGAAGAAATGGCGGATGCTGAAAAAGAGCAAAAACAAAAAAAGAGGAGAAAAAAGGTTCTTCCCCGAGGCACTTCAGAATACCAGGCTGCTTGGATTATCAATGATACAGATGATGAGGAGTCTGTTACGGATGAAGAAGTTGATGATATGGTAATGGATGAAACAGAGAGTGGTCATCCTGGCGAAGAGGGCAATCATTCAGATCTCAGTGAGGATGAAGCTTCTTTAGACTTGAGGGACTCTGATGGAGAAACTGATGCTGATTCAGTGATGATGGATGGTGAAAATTTGACCAAGGAACAGTTGGAGGACGAGATTAAAAAAATAAAGCAAGCCCATGCTGATGATGAGGAATTTCCAGATGAAGTGGATACTCCATTAGACTTTCCAGCTAGAAAACGTTTTGCAAAGTATAGAGGCCTCAAGTCCTTTAGGACCTCTTCATGGGATCCCAAAGAGTCTCTACCTCCAGATTATGCTAGAATCTTTGCCTTCGACAATTTCAACAGGACACAGAAAAGCATCCTTGCTAAAGCTTTAGCTATGGAGGAAGAAAACAGGGATGATTGTGCAACAGCTAGCACATATGCAAGGCTGTACATCAAAGAAGTACCAGCTACTGTTGCTTCTAAATTATGTGTGCGCGCAACTACTATGCCTATCATAGCATGTGGCCTTTTGCAGCATGAATCCAAGATGTCTGTACTCCATTTCAGCTTAAAAAAGCATGACAGTTACAGTGACCCTATCAAAACCAAAGATGAATTAATTTTTCATGTTGGCTTTCGCCAATTTGTTGCTAGGCCAATATTCTCTACTGATAACATGAACTCAGACAAGCACAAAATGGAGAGGTTCCTTCATCCAGGACGTTTTTCCATGGCGTCAATCTATGCTCCTATTACCTTTCCGGCTCTTCCTTTGATAGCTTTCAAGAGTTCTATAGGGGAGGCCACGGCACCTAAAGTTGCTGCTGTTGGTTCTCTGAGAAGCATTGATCCTGACCGGATTATTCTAAAGAAGATAATTTTAACAGGTTATCCTCAACGAGTTTCAAAGAAGAAAGCTGCTGTTAGGTATATGTTCCATAATCCAGAGGATGTGAGATGGTTCAAGCCTGTGGAAGTGTGGAGCAAATGTGGGCGTAGGGGTCGCATCAAGGAGCCTGTTGGTACACATGGGGCAATGAAATGCATCCTTAACGGCACCCTCCAGCAGAACGACACTATATGCATGAGCTTGTACAAGCGCGCATATCCCAAGTGGCCAGACCACCGCTTCCCGCTTCTTGATGCTTGA

Protein Analysis

791

Amino Acids

88.84

Weight (kDa)

6.65

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tsr1_G-like PF22298 119 - 226 2e-06 Tsr1, G-like domain
AARP2CN PF08142 241 - 305 6.4e-20 AARP2CN (NUC121) domain
RIBIOP_C PF04950 481 - 705 5.5e-71 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42440
fragaria_vesca FvH4_5g12620 FvH4_5g12620 FvH4_5g12620
malus_domestica MD06G1211900.v1.1 MD14G1222500.v1.1
prunus_persica Prupe.5G218100_v2.0.a1
pyrus_communis pycom14g18410 pycom14g18420
rosa_chinensis RchiOBHm_Chr7g0184701
rosa_laevigata RLG00000004958
rosa_multiflora Rmu_ssc0000289.1_g000022
rosa_roxburghii Rroxscaffold_3G00269420
rosa_rugosa Rorug06G0464600 Rorug06G0464600
rosa_samantha Rh7AG078400 Rh7BG068900 Rh7CG069700 Rh7DG069100
rosa_wichuraiana Rw7G005700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 869, 2032, 2279
AccBSI CCGCTC 1 cut(s) 137
AccII CGCG 6 cut(s) 13, 43, 64, 260, 1719, 2325
AclI AACGTT 1 cut(s) 1474
AclWI GGATC 9 cut(s) 104, 223, 438, 905, 918, 992, 1517, 1530, 2066
AcoI YGGCCR 2 cut(s) 1064, 2339
AcsI RAATTY 7 cut(s) 144, 274, 629, 647, 900, 1360, 1427
AcuI CTGAAG 5 cut(s) 66, 435, 657, 1025, 1131
AcyI GRCGYC 1 cut(s) 1958
AfaI GTAC 8 cut(s) 356, 375, 802, 1673, 1686, 1779, 2248, 2318
AfiI CCNNNNNNNGG 1 cut(s) 1518
AflIII ACRYGT 1 cut(s) 605
AgsI TTSAA 8 cut(s) 149, 193, 767, 925, 1579, 2008, 2130, 2188
AjnI CCWGG 3 cut(s) 1155, 1252, 1939
AleI CACNNNNGTG 1 cut(s) 548
Alw21I GWGCWC 3 cut(s) 114, 545, 832
Alw26I GTCTC 2 cut(s) 312, 1539
AlwI GGATC 9 cut(s) 104, 223, 438, 905, 918, 992, 1517, 1530, 2066
AlwNI CAGNNNCTG 1 cut(s) 1694
Ama87I CYCGRG 1 cut(s) 1137
AoxI GGCC 6 cut(s) 1064, 1492, 1747, 1877, 2025, 2339
ApeKI GCWGC 5 cut(s) 182, 1160, 1756, 2045, 2141
ApoI RAATTY 7 cut(s) 144, 274, 629, 647, 900, 1360, 1427
ArsI GACNNNNNNTTYG 2 cut(s) 724, 756
AseI ATTAAT 1 cut(s) 1841
AspLEI GCGC 5 cut(s) 45, 1719, 1721, 2325, 2327
AspS9I GGNCC 1 cut(s) 1509
AsuC2I CCSGG 2 cut(s) 792, 874
AsuHPI GGTGA 1 cut(s) 1367
AsuNHI GCTAGC 1 cut(s) 1652
AvaI CYCGRG 1 cut(s) 1137
AvaII GGWCC 1 cut(s) 1509
BalI TGGCCA 1 cut(s) 2341
BamHI GGATCC 2 cut(s) 910, 1522
BanI GGYRCC 3 cut(s) 869, 2032, 2279
BanII GRGCYC 1 cut(s) 255
BauI CACGAG 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 592
Bbv12I GWGCWC 3 cut(s) 114, 545, 832
BbvI GCAGC 5 cut(s) 194, 1147, 1768, 2032, 2128
BccI CCATC 5 cut(s) 745, 1313, 1342, 1346, 2175
BceAI ACGGC 2 cut(s) 2046, 2293
BciT130I CCWGG 3 cut(s) 1157, 1254, 1941
BciVI GTATCC 1 cut(s) 1438
BcnI CCSGG 2 cut(s) 792, 874
BcoDI GTCTC 2 cut(s) 312, 1539
BfaI CTAG 6 cut(s) 132, 615, 1467, 1554, 1653, 1875
BfmI CTRYAG 2 cut(s) 339, 2016
BfuI GTATCC 1 cut(s) 1438
BglI GCCNNNNNGGC 1 cut(s) 446
BglII AGATCT 2 cut(s) 524, 1276
BisI GCNGC 6 cut(s) 138, 183, 1161, 1757, 2046, 2142
BlsI GCNGC 6 cut(s) 139, 184, 1162, 1758, 2047, 2143
Bme1390I CCNGG 5 cut(s) 792, 874, 1157, 1254, 1941
Bme18I GGWCC 1 cut(s) 1509
BmeT110I CYCGRG 1 cut(s) 1137
BmgT120I GGNCC 1 cut(s) 1509
BmiI GGNNCC 8 cut(s) 871, 912, 993, 1524, 1931, 2034, 2238, 2281
BmrFI CCNGG 5 cut(s) 792, 874, 1157, 1254, 1941
BmsI GCATC 7 cut(s) 943, 1078, 1321, 1605, 2238, 2277, 2356
BmtI GCTAGC 1 cut(s) 1656
BoxI GACNNNNGTC 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 592
BpmI CTGGAG 2 cut(s) 1527, 2270
Bpu10I CCTNAGC 1 cut(s) 420
BpuEI CTTGAG 3 cut(s) 890, 1327, 1481
BpuMI CCSGG 2 cut(s) 792, 874
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 2 cut(s) 786, 915
BsaHI GRCGYC 1 cut(s) 1958
BsaJI CCNNGG 8 cut(s) 258, 285, 573, 873, 1138, 1368, 1953, 2028
BsaWI WCCGGW 1 cut(s) 2079
BsaXI ACNNNNNCTCC 2 cut(s) 2197, 2227
Bsc4I CCNNNNNNNGG 1 cut(s) 1518
Bse1I ACTGG 4 cut(s) 731, 820, 858, 883
Bse3DI GCAATG 2 cut(s) 435, 2265
Bse8I GATNNNNATC 2 cut(s) 786, 915
BseBI CCWGG 3 cut(s) 1157, 1254, 1941
BseDI CCNNGG 8 cut(s) 258, 285, 573, 873, 1138, 1368, 1953, 2028
BseJI GATNNNNATC 2 cut(s) 786, 915
BseLI CCNNNNNNNGG 1 cut(s) 1518
BseMI GCAATG 2 cut(s) 435, 2265
BseMII CTCAG 5 cut(s) 353, 729, 1294, 1920, 2051
BseNI ACTGG 4 cut(s) 731, 820, 858, 883
BsePI GCGCGC 2 cut(s) 1717, 2323
BseRI GAGGAG 4 cut(s) 219, 800, 1132, 1205
BseXI GCAGC 5 cut(s) 194, 1147, 1768, 2032, 2128
BsgI GTGCAG 1 cut(s) 56
Bsh1236I CGCG 6 cut(s) 13, 43, 64, 260, 1719, 2325
BshFI GGCC 6 cut(s) 1066, 1494, 1749, 1879, 2027, 2341
BshNI GGYRCC 3 cut(s) 869, 2032, 2279
BsiHKAI GWGCWC 3 cut(s) 114, 545, 832
BsiHKCI CYCGRG 1 cut(s) 1137
BsiSI CCGG 4 cut(s) 791, 873, 1985, 2080
BslFI GGGAC 1 cut(s) 1325
BslI CCNNNNNNNGG 1 cut(s) 1518
BsmAI GTCTC 2 cut(s) 312, 1539
BsmFI GGGAC 1 cut(s) 1325
BsmI GAATGC 1 cut(s) 931
BsnI GGCC 6 cut(s) 1066, 1494, 1749, 1879, 2027, 2341
BsoBI CYCGRG 1 cut(s) 1137
Bsp1286I GDGCHC 4 cut(s) 114, 255, 545, 832
Bsp1407I TGTACA 2 cut(s) 1671, 2316
Bsp143I GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
Bsp19I CCATGG 1 cut(s) 1953
Bsp68I TCGCGA 1 cut(s) 13
BspANI GGCC 6 cut(s) 1066, 1494, 1749, 1879, 2027, 2341
BspCNI CTCAG 5 cut(s) 354, 728, 1293, 1919, 2052
BspFNI CGCG 6 cut(s) 13, 43, 64, 260, 1719, 2325
BspLI GGNNCC 8 cut(s) 871, 912, 993, 1524, 1931, 2034, 2238, 2281
BspOI GCTAGC 1 cut(s) 1656
BspPI GGATC 9 cut(s) 104, 223, 438, 905, 918, 992, 1517, 1530, 2066
BspQI GCTCTTC 1 cut(s) 1995
BspT107I GGYRCC 3 cut(s) 869, 2032, 2279
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 435, 2265
BsrGI TGTACA 2 cut(s) 1671, 2316
BsrI ACTGG 4 cut(s) 731, 820, 858, 883
BssECI CCNNGG 8 cut(s) 258, 285, 573, 873, 1138, 1368, 1953, 2028
BssHII GCGCGC 2 cut(s) 1717, 2323
BssMI GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
BssNI GRCGYC 1 cut(s) 1958
BssSI CACGAG 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 1368, 1953
Bst2BI CACGAG 1 cut(s) 231
Bst2UI CCWGG 3 cut(s) 1157, 1254, 1941
Bst4CI ACNGT 7 cut(s) 340, 373, 547, 1377, 1696, 1808, 1814
Bst6I CTCTTC 4 cut(s) 194, 1254, 1519, 1995
BstACI GRCGYC 1 cut(s) 1958
BstAUI TGTACA 2 cut(s) 1671, 2316
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 8 cut(s) 180, 381, 828, 832, 1407, 1654, 1719, 2325
BstDEI CTNAG 6 cut(s) 362, 420, 715, 1280, 1906, 2060
BstDSI CCRYGG 3 cut(s) 258, 1953, 2028
BstFNI CGCG 6 cut(s) 13, 43, 64, 260, 1719, 2325
BstHHI GCGC 5 cut(s) 45, 1719, 1721, 2325, 2327
BstKTI GATC 9 cut(s) 99, 218, 433, 527, 913, 1000, 1279, 1525, 2074
BstMAI GTCTC 2 cut(s) 312, 1539
BstMBI GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
BstMWI GCNNNNNNNGC 9 cut(s) 70, 128, 137, 277, 425, 446, 736, 1263, 1697
BstNI CCWGG 3 cut(s) 1157, 1254, 1941
BstNSI RCATGY 2 cut(s) 609, 1746
BstPAI GACNNNNGTC 1 cut(s) 592
BstSCI CCNGG 5 cut(s) 790, 872, 1155, 1252, 1939
BstSFI CTRYAG 2 cut(s) 339, 2016
BstUI CGCG 6 cut(s) 13, 43, 64, 260, 1719, 2325
BstV1I GCAGC 5 cut(s) 194, 1147, 1768, 2032, 2128
BstV2I GAAGAC 1 cut(s) 592
BstX2I RGATCY 5 cut(s) 430, 524, 910, 1276, 1522
BstYI RGATCY 5 cut(s) 430, 524, 910, 1276, 1522
BsuI GTATCC 1 cut(s) 1438
BsuRI GGCC 6 cut(s) 1066, 1494, 1749, 1879, 2027, 2341
BtgI CCRYGG 3 cut(s) 258, 1953, 2028
BtsIMutI CAGTG 5 cut(s) 724, 813, 1288, 1347, 1819
BtuMI TCGCGA 1 cut(s) 13
Cac8I GCNNGC 8 cut(s) 180, 381, 828, 832, 1407, 1654, 1719, 2325
CaiI CAGNNNCTG 1 cut(s) 1694
CfoI GCGC 5 cut(s) 45, 1719, 1721, 2325, 2327
Cfr13I GGNCC 1 cut(s) 1509
Cfr42I CCGCGG 1 cut(s) 261
CseI GACGC 3 cut(s) 49, 728, 1947
Csp6I GTAC 8 cut(s) 355, 374, 801, 1672, 1685, 1778, 2247, 2317
CspCI CAANNNNNGTGG 2 cut(s) 903, 938
CviQI GTAC 8 cut(s) 355, 374, 801, 1672, 1685, 1778, 2247, 2317
DdeI CTNAG 6 cut(s) 362, 420, 715, 1280, 1906, 2060
DpnI GATC 9 cut(s) 98, 217, 432, 526, 912, 999, 1278, 1524, 2073
DpnII GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
EaeI YGGCCR 2 cut(s) 1064, 2339
Eam1104I CTCTTC 4 cut(s) 194, 1254, 1519, 1995
EarI CTCTTC 4 cut(s) 194, 1254, 1519, 1995
EciI GGCGGA 1 cut(s) 1100
Eco130I CCWWGG 2 cut(s) 1368, 1953
Eco147I AGGCCT 1 cut(s) 1494
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 1509
Eco57I CTGAAG 5 cut(s) 66, 435, 657, 1025, 1131
Eco88I CYCGRG 1 cut(s) 1137
EcoO109I RGGNCCY 1 cut(s) 1509
EcoRI GAATTC 1 cut(s) 144
EcoRII CCWGG 3 cut(s) 1155, 1252, 1939
EcoT14I CCWWGG 2 cut(s) 1368, 1953
EcoT22I ATGCAT 2 cut(s) 2270, 2309
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 2 cut(s) 1368, 1953
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 1 cut(s) 1325
FauI CCCGC 2 cut(s) 171, 2364
FauNDI CATATG 1 cut(s) 1660
Fnu4HI GCNGC 6 cut(s) 138, 183, 1161, 1757, 2046, 2142
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 6 cut(s) 138, 183, 1161, 1757, 2046, 2142
FspBI CTAG 6 cut(s) 132, 615, 1467, 1554, 1653, 1875
GlaI GCGC 5 cut(s) 44, 1718, 1720, 2324, 2326
GluI GCNGC 6 cut(s) 138, 183, 1161, 1757, 2046, 2142
GsuI CTGGAG 2 cut(s) 1527, 2270
HaeIII GGCC 6 cut(s) 1066, 1494, 1749, 1879, 2027, 2341
HapII CCGG 4 cut(s) 791, 873, 1985, 2080
HgaI GACGC 3 cut(s) 49, 728, 1947
HhaI GCGC 5 cut(s) 45, 1719, 1721, 2325, 2327
Hin1I GRCGYC 1 cut(s) 1958
Hin6I GCGC 5 cut(s) 43, 1717, 1719, 2323, 2325
HinP1I GCGC 5 cut(s) 43, 1717, 1719, 2323, 2325
HindIII AAGCTT 2 cut(s) 1293, 1608
HpaII CCGG 4 cut(s) 791, 873, 1985, 2080
HphI GGTGA 1 cut(s) 1367
Hpy166II GTNNAC 3 cut(s) 25, 536, 2249
Hpy8I GTNNAC 3 cut(s) 25, 536, 2249
Hpy99I CGWCG 2 cut(s) 64, 551
HpyAV CCTTC 5 cut(s) 187, 464, 815, 1576, 1943
HpyCH4III ACNGT 7 cut(s) 340, 373, 547, 1377, 1696, 1808, 1814
HpyCH4IV ACGT 3 cut(s) 559, 1474, 1945
HpyF10VI GCNNNNNNNGC 9 cut(s) 70, 128, 137, 277, 425, 446, 736, 1263, 1697
HpyF3I CTNAG 6 cut(s) 362, 420, 715, 1280, 1906, 2060
HpySE526I ACGT 3 cut(s) 559, 1474, 1945
Hsp92I GRCGYC 1 cut(s) 1958
HspAI GCGC 5 cut(s) 43, 1717, 1719, 2323, 2325
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
LguI GCTCTTC 1 cut(s) 1995
LmnI GCTCC 7 cut(s) 109, 128, 169, 703, 1975, 2205, 2236
Lsp1109I GCAGC 5 cut(s) 194, 1147, 1768, 2032, 2128
LweI GCATC 7 cut(s) 943, 1078, 1321, 1605, 2238, 2277, 2356
MaeI CTAG 6 cut(s) 132, 615, 1467, 1554, 1653, 1875
MaeII ACGT 3 cut(s) 559, 1474, 1945
MaeIII GTNAC 3 cut(s) 1198, 1808, 1814
MalI GATC 9 cut(s) 98, 217, 432, 526, 912, 999, 1278, 1524, 2073
MbiI CCGCTC 1 cut(s) 137
MboI GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
MflI RGATCY 5 cut(s) 430, 524, 910, 1276, 1522
MhlI GDGCHC 4 cut(s) 114, 255, 545, 832
MlsI TGGCCA 1 cut(s) 2341
MluNI TGGCCA 1 cut(s) 2341
MlyI GAGTC 5 cut(s) 485, 602, 1202, 1307, 1541
MmeI TCCRAC 1 cut(s) 1359
Mox20I TGGCCA 1 cut(s) 2341
Mph1103I ATGCAT 2 cut(s) 2270, 2309
MscI TGGCCA 1 cut(s) 2341
MseI TTAA 6 cut(s) 699, 1392, 1793, 1841, 2105, 2274
MslI CAYNNNNRTG 2 cut(s) 548, 1416
Msp20I TGGCCA 1 cut(s) 2341
MspA1I CMGCKG 3 cut(s) 260, 659, 1003
MspI CCGG 4 cut(s) 791, 873, 1985, 2080
MspR9I CCNGG 5 cut(s) 792, 874, 1157, 1254, 1941
Mva1269I GAATGC 1 cut(s) 931
MvaI CCWGG 3 cut(s) 1157, 1254, 1941
MvnI CGCG 6 cut(s) 13, 43, 64, 260, 1719, 2325
MwoI GCNNNNNNNGC 9 cut(s) 70, 128, 137, 277, 425, 446, 736, 1263, 1697
NciI CCSGG 2 cut(s) 792, 874
NcoI CCATGG 1 cut(s) 1953
NdeI CATATG 1 cut(s) 1660
NdeII GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
NheI GCTAGC 1 cut(s) 1652
NlaIV GGNNCC 8 cut(s) 871, 912, 993, 1524, 1931, 2034, 2238, 2281
NmuCI GTSAC 1 cut(s) 1814
NruI TCGCGA 1 cut(s) 13
NsiI ATGCAT 2 cut(s) 2270, 2309
NspI RCATGY 2 cut(s) 609, 1746
OliI CACNNNNGTG 1 cut(s) 548
PauI GCGCGC 2 cut(s) 1717, 2323
PceI AGGCCT 1 cut(s) 1494
PciI ACATGT 1 cut(s) 605
PciSI GCTCTTC 1 cut(s) 1995
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 56
PctI GAATGC 1 cut(s) 931
PfeI GAWTC 6 cut(s) 67, 491, 787, 1337, 1557, 1763
PfoI TCCNGGA 2 cut(s) 790, 1939
PkrI GCNGC 6 cut(s) 139, 184, 1162, 1758, 2047, 2143
PleI GAGTC 5 cut(s) 484, 601, 1201, 1307, 1540
PpsI GAGTC 5 cut(s) 484, 601, 1201, 1307, 1540
Ppu21I YACGTR 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 1509
PscI ACATGT 1 cut(s) 605
PshAI GACNNNNGTC 1 cut(s) 592
PshBI ATTAAT 1 cut(s) 1841
Psp1406I AACGTT 1 cut(s) 1474
Psp5II RGGWCCY 1 cut(s) 1509
Psp6I CCWGG 3 cut(s) 1155, 1252, 1939
PspGI CCWGG 3 cut(s) 1155, 1252, 1939
PspN4I GGNNCC 8 cut(s) 871, 912, 993, 1524, 1931, 2034, 2238, 2281
PspPI GGNCC 1 cut(s) 1509
PspPPI RGGWCCY 1 cut(s) 1509
PstNI CAGNNNCTG 1 cut(s) 1694
PsuI RGATCY 5 cut(s) 430, 524, 910, 1276, 1522
PteI GCGCGC 2 cut(s) 1717, 2323
PvuII CAGCTG 1 cut(s) 659
RruI TCGCGA 1 cut(s) 13
RsaI GTAC 8 cut(s) 356, 375, 802, 1673, 1686, 1779, 2248, 2318
RsaNI GTAC 8 cut(s) 355, 374, 801, 1672, 1685, 1778, 2247, 2317
RseI CAYNNNNRTG 2 cut(s) 548, 1416
SacII CCGCGG 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 1995
SaqAI TTAA 6 cut(s) 699, 1392, 1793, 1841, 2105, 2274
SatI GCNGC 6 cut(s) 138, 183, 1161, 1757, 2046, 2142
Sau3AI GATC 9 cut(s) 96, 215, 430, 524, 910, 997, 1276, 1522, 2071
Sau96I GGNCC 1 cut(s) 1509
SchI GAGTC 5 cut(s) 485, 602, 1202, 1307, 1541
ScrFI CCNGG 5 cut(s) 792, 874, 1157, 1254, 1941
SduI GDGCHC 4 cut(s) 114, 255, 545, 832
SfaNI GCATC 7 cut(s) 943, 1078, 1321, 1605, 2238, 2277, 2356
SfcI CTRYAG 2 cut(s) 339, 2016
Sfr303I CCGCGG 1 cut(s) 261
SgrBI CCGCGG 1 cut(s) 261
SinI GGWCC 1 cut(s) 1509
SmiMI CAYNNNNRTG 2 cut(s) 548, 1416
SmlI CTYRAG 3 cut(s) 905, 1306, 1496
SmoI CTYRAG 3 cut(s) 905, 1306, 1496
SseBI AGGCCT 1 cut(s) 1494
SspI AATATT 1 cut(s) 1884
SspMI CTAG 6 cut(s) 132, 615, 1467, 1554, 1653, 1875
StuI AGGCCT 1 cut(s) 1494
StyD4I CCNGG 5 cut(s) 790, 872, 1155, 1252, 1939
StyI CCWWGG 2 cut(s) 1368, 1953
TaaI ACNGT 7 cut(s) 340, 373, 547, 1377, 1696, 1808, 1814
TaiI ACGT 3 cut(s) 562, 1477, 1948
TaqI TCGA 3 cut(s) 142, 209, 1569
TatI WGTACW 4 cut(s) 354, 1671, 1777, 2316
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 6 cut(s) 67, 491, 787, 1337, 1557, 1763
Tru1I TTAA 6 cut(s) 699, 1392, 1793, 1841, 2105, 2274
Tru9I TTAA 6 cut(s) 699, 1392, 1793, 1841, 2105, 2274
TscAI CASTG 5 cut(s) 731, 820, 1288, 1347, 1819
TseFI GTSAC 1 cut(s) 1814
TseI GCWGC 5 cut(s) 182, 1160, 1756, 2045, 2141
Tsp45I GTSAC 1 cut(s) 1814
TspGWI ACGGA 1 cut(s) 1217
TspRI CASTG 5 cut(s) 731, 820, 1288, 1347, 1819
VpaK11BI GGWCC 1 cut(s) 1509
VspI ATTAAT 1 cut(s) 1841
XapI RAATTY 7 cut(s) 144, 274, 629, 647, 900, 1360, 1427
XceI RCATGY 2 cut(s) 609, 1746
XcmI CCANNNNNNNNNTGG 1 cut(s) 1375
XspI CTAG 6 cut(s) 132, 615, 1467, 1554, 1653, 1875
Zsp2I ATGCAT 2 cut(s) 2270, 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.