FvH4_5g12900

mizu-kussei

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015885
Physical Location & Seq
Reverse (-)
7282065 .. 7283822
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12900.t1

Sequence Viewer

Length: 1056 bp
ATGTCTGGTGTGAATAGTGATGGCTTTGCTCTTTTTCCACTGTGGGTTTTTGGATTCTCTTGGGGCAGCTCTGCTAGAAACAAAGAGAGAATTAATGTAGAAACACCACACCACCACCTCTCCCTCAATAAAAGAGAAGAAACCAAACTCCTCTCACTCTCTCTTCTAGTCTTTACTCTTTACTCAACACCAACCTTCTTTAACAACACTGCCTCTCTTCCATTCATTGCTTTCCTCTCTTATGCTACAACACCAACACCAATGGCAAAAAGCTTGCAGGACTCTTCCTCCAAAAGACACTTCCACTGGACCAACAAAGTTGGCAATGAAGATGATCATGGCCATGATGACCATGCTCTTCCTTCTTTGCCAAAAGCAGTGTCTGATGAAGAGAAGAGGAAGGACACTCCTCACAAGGCGATTCAGCTGCAGAGGAGGAAGCTCCAGGCAGCAGCAATATCGAGGCTGCGGTCGGTGCTCACGGCCTTTGGGAAGAACAGGGGAGGCCTTCCACTGGGTCTGGGGTCTAGAGTTGTAGGGACCCTTTTCGGCTCTAAGAAAGGGCATGTGCATTTTGCTCTCCAGAGAGACCCAAACTCACACCCAGCTTTCCTGATTGAGCTTGCTACTCCAATCAGTGGTCTGGTGAAGGAAATGGCTTCCGGGCTTGTGAGGATTGCTTTGGAGTGTGACAAGGAGAAGGAAGATCAGGAGAAGAAGAAAACTATGGAGTCGAAATCGTCGAAATCTGGTTCAGCTGTGAGGAGGTTGCTGGAGGAGCCTGTGTGGAGGACTTACTGCAATGGGAAGAAGTCTGGGTTTGCTAGCAGGAGGGAATGTGGGCCCAAAGAGTGGAAGGTGTTGAAAGCTGTTGAGCCAATTTCAATGGGTGCTGGGGTGTTGCCTTGTGGGGATGAAGGTGAAGATGGTGAGCTCATGTACATGAGGGCCAAGTTTGAGAGGGTTGTTGGGTCCAGAGACTCTGAGGCTTTCTACATGATGAACCCTGACAGCAATGGAGCTCCTGAACTCAGTATCTACTTGCTTCGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

352

Amino Acids

39.0

Weight (kDa)

9.35

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 178 - 350 2.7e-68 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23100
fragaria_vesca FvH4_5g12900
malus_domestica MD06G1209400.v1.1 MD14G1219900.v1.1
prunus_persica Prupe.5G214500_v2.0.a1
pyrus_communis pycom06g18700
rosa_chinensis RchiOBHm_Chr7g0184341
rosa_laevigata RLG00000004985
rosa_multiflora Rmu_sc0001557.1_g000024
rosa_roxburghii Rroxscaffold_3G00269710
rosa_samantha Rh7AG080900 Rh7BG066300 Rh7CG066600 Rh7DG066300
rosa_wichuraiana Rw7G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 638, 852
AciI CCGC 1 cut(s) 469
AcoI YGGCCR 1 cut(s) 340
AfaI GTAC 1 cut(s) 941
AfiI CCNNNNNNNGG 3 cut(s) 514, 638, 852
AgsI TTSAA 2 cut(s) 865, 885
AjnI CCWGG 1 cut(s) 444
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
Alw21I GWGCWC 3 cut(s) 480, 936, 1024
Alw26I GTCTC 2 cut(s) 582, 972
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 5 cut(s) 340, 483, 505, 842, 948
ApaI GGGCCC 1 cut(s) 846
ApeKI GCWGC 5 cut(s) 66, 427, 449, 452, 466
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AseI ATTAAT 1 cut(s) 93
AspS9I GGNCC 6 cut(s) 309, 540, 842, 843, 948, 972
AsuC2I CCSGG 1 cut(s) 664
AsuHPI GGTGA 3 cut(s) 658, 932, 941
AsuNHI GCTAGC 1 cut(s) 824
AvaII GGWCC 3 cut(s) 309, 540, 972
BaeGI GKGCMC 1 cut(s) 846
BalI TGGCCA 1 cut(s) 342
BanII GRGCYC 3 cut(s) 846, 936, 1024
Bbv12I GWGCWC 3 cut(s) 480, 936, 1024
BbvI GCAGC 5 cut(s) 78, 414, 453, 461, 464
BccI CCATC 2 cut(s) 14, 920
BceAI ACGGC 1 cut(s) 498
BcgI CGANNNNNNTGC 4 cut(s) 409, 441, 443, 475
BciT130I CCWGG 1 cut(s) 446
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 664
BcoDI GTCTC 2 cut(s) 582, 972
BfaI CTAG 4 cut(s) 75, 167, 528, 825
BfmI CTRYAG 1 cut(s) 428
BisI GCNGC 5 cut(s) 67, 428, 450, 453, 467
BlsI GCNGC 5 cut(s) 68, 429, 451, 454, 468
Bme1390I CCNGG 2 cut(s) 446, 664
Bme18I GGWCC 3 cut(s) 309, 540, 972
BmgT120I GGNCC 6 cut(s) 309, 540, 842, 843, 948, 972
BmiI GGNNCC 5 cut(s) 541, 542, 780, 844, 973
BmrFI CCNGG 2 cut(s) 446, 664
BmrI ACTGGG 1 cut(s) 524
BmtI GCTAGC 1 cut(s) 828
BmuI ACTGGG 1 cut(s) 524
BpmI CTGGAG 3 cut(s) 428, 566, 794
BpuMI CCSGG 1 cut(s) 664
BsaI GGTCTC 1 cut(s) 582
BsaXI ACNNNNNCTCC 8 cut(s) 104, 134, 272, 302, 770, 800, 823, 853
Bsc4I CCNNNNNNNGG 3 cut(s) 514, 638, 852
Bse1I ACTGG 2 cut(s) 311, 519
Bse3DI GCAATG 4 cut(s) 225, 331, 808, 1021
BseBI CCWGG 1 cut(s) 446
BseGI GGATG 1 cut(s) 919
BseLI CCNNNNNNNGG 3 cut(s) 514, 638, 852
BseMI GCAATG 4 cut(s) 225, 331, 808, 1021
BseMII CTCAG 2 cut(s) 975, 1045
BseNI ACTGG 2 cut(s) 311, 519
BseRI GAGGAG 5 cut(s) 140, 399, 448, 778, 791
BseSI GKGCMC 1 cut(s) 846
BseXI GCAGC 5 cut(s) 78, 414, 453, 461, 464
BseYI CCCAGC 2 cut(s) 604, 893
Bsh1285I CGRYCG 1 cut(s) 473
BshFI GGCC 5 cut(s) 342, 485, 507, 844, 950
BsiEI CGRYCG 1 cut(s) 473
BsiHKAI GWGCWC 3 cut(s) 480, 936, 1024
BsiSI CCGG 1 cut(s) 663
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 3 cut(s) 514, 638, 852
BsmAI GTCTC 2 cut(s) 582, 972
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 5 cut(s) 342, 485, 507, 844, 950
Bso31I GGTCTC 1 cut(s) 582
Bsp120I GGGCCC 1 cut(s) 842
Bsp1286I GDGCHC 4 cut(s) 480, 846, 936, 1024
Bsp1407I TGTACA 1 cut(s) 939
Bsp143I GATC 2 cut(s) 334, 706
BspACI CCGC 1 cut(s) 469
BspANI GGCC 5 cut(s) 342, 485, 507, 844, 950
BspCNI CTCAG 2 cut(s) 976, 1044
BspLI GGNNCC 5 cut(s) 541, 542, 780, 844, 973
BspMAI CTGCAG 1 cut(s) 432
BspOI GCTAGC 1 cut(s) 828
BspQI GCTCTTC 1 cut(s) 363
BspTNI GGTCTC 1 cut(s) 582
BsrDI GCAATG 4 cut(s) 225, 331, 808, 1021
BsrGI TGTACA 1 cut(s) 939
BsrI ACTGG 2 cut(s) 311, 519
BssMI GATC 2 cut(s) 334, 706
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 6 cut(s) 168, 222, 289, 363, 384, 389
BstAUI TGTACA 1 cut(s) 939
BstC8I GCNNGC 3 cut(s) 275, 624, 826
BstDEI CTNAG 3 cut(s) 555, 984, 1031
BstF5I GGATG 1 cut(s) 919
BstKTI GATC 2 cut(s) 337, 709
BstMAI GTCTC 2 cut(s) 582, 972
BstMBI GATC 2 cut(s) 334, 706
BstMCI CGRYCG 1 cut(s) 473
BstMWI GCNNNNNNNGC 2 cut(s) 475, 778
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 1 cut(s) 569
BstSCI CCNGG 2 cut(s) 444, 662
BstSFI CTRYAG 1 cut(s) 428
BstSLI GKGCMC 1 cut(s) 846
BstV1I GCAGC 5 cut(s) 78, 414, 453, 461, 464
BsuRI GGCC 5 cut(s) 342, 485, 507, 844, 950
BtsCI GGATG 1 cut(s) 919
BtsI GCAGTG 2 cut(s) 207, 384
BtsIMutI CAGTG 6 cut(s) 38, 207, 304, 384, 512, 643
Cac8I GCNNGC 3 cut(s) 275, 624, 826
CaiI CAGNNNCTG 1 cut(s) 383
Cfr13I GGNCC 6 cut(s) 309, 540, 842, 843, 948, 972
Csp6I GTAC 1 cut(s) 940
CviAII CATG 7 cut(s) 338, 344, 353, 566, 937, 943, 997
CviQI GTAC 1 cut(s) 940
DdeI CTNAG 3 cut(s) 555, 984, 1031
DpnI GATC 2 cut(s) 336, 708
DpnII GATC 2 cut(s) 334, 706
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 6 cut(s) 168, 222, 289, 363, 384, 389
EarI CTCTTC 6 cut(s) 168, 222, 289, 363, 384, 389
Ecl136II GAGCTC 2 cut(s) 934, 1022
Eco147I AGGCCT 1 cut(s) 507
Eco24I GRGCYC 3 cut(s) 846, 936, 1024
Eco31I GGTCTC 1 cut(s) 582
Eco47I GGWCC 3 cut(s) 309, 540, 972
Eco53kI GAGCTC 2 cut(s) 934, 1022
EcoICRI GAGCTC 2 cut(s) 934, 1022
EcoO109I RGGNCCY 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 444
EcoT38I GRGCYC 3 cut(s) 846, 936, 1024
FaeI CATG 7 cut(s) 341, 347, 356, 569, 940, 946, 1000
FaiI YATR 9 cut(s) 243, 339, 345, 354, 567, 728, 938, 944, 998
FaqI GGGAC 1 cut(s) 553
FatI CATG 7 cut(s) 337, 343, 352, 565, 936, 942, 996
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 5 cut(s) 67, 428, 450, 453, 467
FokI GGATG 1 cut(s) 926
FriOI GRGCYC 3 cut(s) 846, 936, 1024
Fsp4HI GCNGC 5 cut(s) 67, 428, 450, 453, 467
FspBI CTAG 4 cut(s) 75, 167, 528, 825
GluI GCNGC 5 cut(s) 67, 428, 450, 453, 467
GsaI CCCAGC 2 cut(s) 608, 897
GsuI CTGGAG 3 cut(s) 428, 566, 794
HaeIII GGCC 5 cut(s) 342, 485, 507, 844, 950
HapII CCGG 1 cut(s) 663
Hin1II CATG 7 cut(s) 341, 347, 356, 569, 940, 946, 1000
HindIII AAGCTT 1 cut(s) 271
HinfI GANTC 6 cut(s) 54, 281, 421, 731, 980, 1050
HpaII CCGG 1 cut(s) 663
HphI GGTGA 3 cut(s) 658, 932, 941
Hpy188I TCNGA 2 cut(s) 385, 985
Hpy188III TCNNGA 6 cut(s) 528, 583, 613, 710, 975, 1025
Hpy99I CGWCG 1 cut(s) 745
HpyAV CCTTC 8 cut(s) 205, 372, 394, 518, 643, 694, 850, 911
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 277, 430, 571, 801
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 778
HpyF3I CTNAG 3 cut(s) 555, 984, 1031
Hsp92II CATG 7 cut(s) 341, 347, 356, 569, 940, 946, 1000
KflI GGGWCCC 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 334, 706
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 4 cut(s) 447, 778, 1019, 1027
Lsp1109I GCAGC 5 cut(s) 78, 414, 453, 461, 464
MaeI CTAG 4 cut(s) 75, 167, 528, 825
MaeIII GTNAC 1 cut(s) 689
MalI GATC 2 cut(s) 336, 708
MboI GATC 2 cut(s) 334, 706
MhlI GDGCHC 4 cut(s) 480, 846, 936, 1024
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 2 cut(s) 90, 879
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 3 cut(s) 275, 740, 974
Mox20I TGGCCA 1 cut(s) 342
MscI TGGCCA 1 cut(s) 342
MseI TTAA 2 cut(s) 93, 201
MslI CAYNNNNRTG 2 cut(s) 342, 941
Msp20I TGGCCA 1 cut(s) 342
MspA1I CMGCKG 2 cut(s) 427, 758
MspI CCGG 1 cut(s) 663
MspR9I CCNGG 2 cut(s) 446, 664
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 2 cut(s) 475, 778
NciI CCSGG 1 cut(s) 664
NdeII GATC 2 cut(s) 334, 706
NheI GCTAGC 1 cut(s) 824
NlaIII CATG 7 cut(s) 341, 347, 356, 569, 940, 946, 1000
NlaIV GGNNCC 5 cut(s) 541, 542, 780, 844, 973
NmuCI GTSAC 1 cut(s) 689
NspI RCATGY 1 cut(s) 569
PceI AGGCCT 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 740
PfeI GAWTC 2 cut(s) 54, 421
PflMI CCANNNNNTGG 2 cut(s) 638, 852
PkrI GCNGC 5 cut(s) 68, 429, 451, 454, 468
PleI GAGTC 3 cut(s) 275, 739, 974
PpsI GAGTC 3 cut(s) 275, 739, 974
PpuMI RGGWCCY 1 cut(s) 540
PshBI ATTAAT 1 cut(s) 93
Psp124BI GAGCTC 2 cut(s) 936, 1024
Psp5II RGGWCCY 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 444
PspFI CCCAGC 2 cut(s) 604, 893
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 5 cut(s) 541, 542, 780, 844, 973
PspOMI GGGCCC 1 cut(s) 842
PspPI GGNCC 6 cut(s) 309, 540, 842, 843, 948, 972
PspPPI RGGWCCY 1 cut(s) 540
PstI CTGCAG 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 383
PvuII CAGCTG 2 cut(s) 427, 758
RsaI GTAC 1 cut(s) 941
RsaNI GTAC 1 cut(s) 940
RseI CAYNNNNRTG 2 cut(s) 342, 941
SacI GAGCTC 2 cut(s) 936, 1024
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 93, 201
SatI GCNGC 5 cut(s) 67, 428, 450, 453, 467
Sau3AI GATC 2 cut(s) 334, 706
Sau96I GGNCC 6 cut(s) 309, 540, 842, 843, 948, 972
SchI GAGTC 3 cut(s) 275, 740, 974
ScrFI CCNGG 2 cut(s) 446, 664
SduI GDGCHC 4 cut(s) 480, 846, 936, 1024
SfcI CTRYAG 1 cut(s) 428
SinI GGWCC 3 cut(s) 309, 540, 972
SmiMI CAYNNNNRTG 2 cut(s) 342, 941
Sse9I AATT 2 cut(s) 90, 879
SseBI AGGCCT 1 cut(s) 507
SsiI CCGC 1 cut(s) 469
SspMI CTAG 4 cut(s) 75, 167, 528, 825
SstI GAGCTC 2 cut(s) 936, 1024
StuI AGGCCT 1 cut(s) 507
StyD4I CCNGG 2 cut(s) 444, 662
TaaI ACNGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 461, 734, 743, 1048
TasI AATT 2 cut(s) 90, 879
TatI WGTACW 1 cut(s) 939
TfiI GAWTC 2 cut(s) 54, 421
Tru1I TTAA 2 cut(s) 93, 201
Tru9I TTAA 2 cut(s) 93, 201
TscAI CASTG 6 cut(s) 45, 214, 311, 384, 519, 643
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 5 cut(s) 66, 427, 449, 452, 466
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 5 cut(s) 214, 342, 402, 930, 1016
TspRI CASTG 6 cut(s) 45, 214, 311, 384, 519, 643
Van91I CCANNNNNTGG 2 cut(s) 638, 852
VpaK11BI GGWCC 3 cut(s) 309, 540, 972
VspI ATTAAT 1 cut(s) 93
XbaI TCTAGA 1 cut(s) 527
XceI RCATGY 1 cut(s) 569
XspI CTAG 4 cut(s) 75, 167, 528, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.