RLG00000004985

mizu-kussei

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015885
Physical Location & Seq
Reverse (-)
64181823 .. 64182665
843 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004985

Sequence Viewer

Length: 843 bp
ATGGCAAAGAGCTTGCAGGACTCCTCTTCCAAAAGACACTTCCACTGGACCAACAAAGTTGGCAATGAAGATGATCATGATCATGATAACCATGAAGATGGAGTTGGAGTTGGAGTTGGAGTAGGTCTTCCAACCTTGAAATCTTCTCTGTCAAAGGCAGTTTCTGATGAGGAAGAGAAGAGAAAGGAGGGTCAAGGGACTCAAAAGGTTCAGCTACAGAGAAGGAAGCTCCAGGCAGCAGCAATTTCGAGGCTCCGGTCGGTGCTGACCGCGTTTGGGAAGAACAGGTCAGGGCTCCCACTGGGTCTGGGGCCGAGAGTTATAGGGACCCTTTTTGGGTCTAAGAAAGGGCATGTGCATTTTGCTCTCCAGAGAGACCCAAATTCACACCCGGCTTTCCTGATTGAGCTTGCTACTCCAATTAGTGGGCTGGTGAAGGAAATGGCTTCTGGGCTTGTGAGGATTGCTTTGGAGTGTGACAAGGAGAAGGAGGAGGAGAAGAAAACAGAGGATTCGAAATCGAAGTCGAAAAGTTCAGCTGTGAGGTTGCTGGAGGAGCCTGTGTGGAGGACTTACTGCAATGGGAAGAAGTCTGGGTTTGCTAGCAGGAGGGAATGTGGGCCTAAAGAGTGGAAGGTGTTGAAGGCTGTTGAGCCCATTTCAATGGGTGCTGGGGTCTTGCCTCCTGGGAATGAAGGTGAAGCTGGGTGTGATGGTGAGCTCATGTATATGAGGGCCAAGTTCGAGAGGGTTGTTGGGTCTAGAGACTCTGAGGCTTTCTACATGATGAACCCAGATAGCAATGGAGCTCCTGAACTCAGTATCTACTTGCTTCGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

281

Amino Acids

30.71

Weight (kDa)

9.03

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 107 - 279 1.9e-68 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23100
fragaria_vesca FvH4_5g12900
malus_domestica MD06G1209400.v1.1 MD14G1219900.v1.1
prunus_persica Prupe.5G214500_v2.0.a1
pyrus_communis pycom06g18700
rosa_chinensis RchiOBHm_Chr7g0184341
rosa_laevigata RLG00000004985
rosa_multiflora Rmu_sc0001557.1_g000024
rosa_roxburghii Rroxscaffold_3G00269710
rosa_samantha Rh7AG080900 Rh7BG066300 Rh7CG066600 Rh7DG066300
rosa_wichuraiana Rw7G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 425
AccII CGCG 1 cut(s) 272
AciI CCGC 1 cut(s) 270
AcsI RAATTY 1 cut(s) 382
AfiI CCNNNNNNNGG 3 cut(s) 276, 336, 425
AgsI TTSAA 3 cut(s) 139, 643, 663
AjnI CCWGG 2 cut(s) 231, 685
AjuI GAANNNNNNNTTGG 4 cut(s) 23, 55, 87, 119
AluBI AGCT 8 cut(s) 12, 214, 229, 409, 539, 704, 721, 809
AluI AGCT 8 cut(s) 12, 214, 229, 409, 539, 704, 721, 809
Alw21I GWGCWC 2 cut(s) 723, 811
Alw26I GTCTC 2 cut(s) 369, 759
AlwNI CAGNNNCTG 1 cut(s) 164
AoxI GGCC 3 cut(s) 311, 620, 735
ApeKI GCWGC 2 cut(s) 236, 239
ApoI RAATTY 1 cut(s) 382
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
AspS9I GGNCC 5 cut(s) 48, 311, 327, 620, 735
AsuC2I CCSGG 1 cut(s) 392
AsuHPI GGTGA 3 cut(s) 445, 710, 728
AsuII TTCGAA 1 cut(s) 515
AsuNHI GCTAGC 1 cut(s) 602
AvaII GGWCC 2 cut(s) 48, 327
BanII GRGCYC 4 cut(s) 297, 657, 723, 811
BbsI GAAGAC 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 723, 811
BbvI GCAGC 2 cut(s) 248, 251
BccI CCATC 2 cut(s) 92, 707
BcgI CGANNNNNNTGC 2 cut(s) 228, 262
BciT130I CCWGG 2 cut(s) 233, 687
BclI TGATCA 2 cut(s) 73, 79
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 2 cut(s) 369, 759
BfaI CTAG 2 cut(s) 603, 762
BfmI CTRYAG 1 cut(s) 215
BisI GCNGC 2 cut(s) 237, 240
BlsI GCNGC 2 cut(s) 238, 241
Bme1390I CCNGG 3 cut(s) 233, 392, 687
Bme18I GGWCC 2 cut(s) 48, 327
BmgT120I GGNCC 5 cut(s) 48, 311, 327, 620, 735
BmiI GGNNCC 6 cut(s) 254, 296, 312, 328, 329, 558
BmrFI CCNGG 3 cut(s) 233, 392, 687
BmrI ACTGGG 1 cut(s) 311
BmtI GCTAGC 1 cut(s) 606
BmuI ACTGGG 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 119
BpmI CTGGAG 3 cut(s) 215, 353, 572
Bpu14I TTCGAA 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 686
BsaWI WCCGGW 1 cut(s) 255
Bsc4I CCNNNNNNNGG 3 cut(s) 276, 336, 425
Bse1I ACTGG 2 cut(s) 50, 306
Bse3DI GCAATG 3 cut(s) 70, 586, 808
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 2 cut(s) 233, 687
BseDI CCNNGG 1 cut(s) 686
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 3 cut(s) 276, 336, 425
BseMI GCAATG 3 cut(s) 70, 586, 808
BseMII CTCAG 2 cut(s) 762, 832
BseNI ACTGG 2 cut(s) 50, 306
BseRI GAGGAG 4 cut(s) 13, 506, 509, 569
BseXI GCAGC 2 cut(s) 248, 251
BseYI CCCAGC 2 cut(s) 671, 704
Bsh1236I CGCG 1 cut(s) 272
Bsh1285I CGRYCG 1 cut(s) 260
BshFI GGCC 3 cut(s) 313, 622, 737
BsiEI CGRYCG 1 cut(s) 260
BsiHKAI GWGCWC 2 cut(s) 723, 811
BsiSI CCGG 2 cut(s) 256, 392
BslFI GGGAC 2 cut(s) 211, 340
BslI CCNNNNNNNGG 3 cut(s) 276, 336, 425
BsmAI GTCTC 2 cut(s) 369, 759
BsmFI GGGAC 2 cut(s) 211, 340
BsnI GGCC 3 cut(s) 313, 622, 737
Bso31I GGTCTC 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 4 cut(s) 297, 657, 723, 811
Bsp143I GATC 2 cut(s) 73, 79
BspACI CCGC 1 cut(s) 270
BspANI GGCC 3 cut(s) 313, 622, 737
BspCNI CTCAG 2 cut(s) 763, 831
BspFNI CGCG 1 cut(s) 272
BspHI TCATGA 2 cut(s) 76, 82
BspLI GGNNCC 6 cut(s) 254, 296, 312, 328, 329, 558
BspOI GCTAGC 1 cut(s) 606
BspT104I TTCGAA 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 369
BsrDI GCAATG 3 cut(s) 70, 586, 808
BsrI ACTGG 2 cut(s) 50, 306
BssECI CCNNGG 1 cut(s) 686
BssMI GATC 2 cut(s) 73, 79
Bst2UI CCWGG 2 cut(s) 233, 687
Bst6I CTCTTC 3 cut(s) 31, 168, 173
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 14, 411, 604
BstDEI CTNAG 3 cut(s) 342, 771, 818
BstFNI CGCG 1 cut(s) 272
BstKTI GATC 2 cut(s) 76, 82
BstMAI GTCTC 2 cut(s) 369, 759
BstMBI GATC 2 cut(s) 73, 79
BstMCI CGRYCG 1 cut(s) 260
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNI CCWGG 2 cut(s) 233, 687
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 231, 390, 685
BstSFI CTRYAG 1 cut(s) 215
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 2 cut(s) 248, 251
BstV2I GAAGAC 1 cut(s) 119
BstXI CCANNNNNNTGG 2 cut(s) 98, 664
BsuRI GGCC 3 cut(s) 313, 622, 737
BtsIMutI CAGTG 2 cut(s) 43, 299
Cac8I GCNNGC 3 cut(s) 14, 411, 604
CaiI CAGNNNCTG 1 cut(s) 164
CciI TCATGA 2 cut(s) 76, 82
Cfr13I GGNCC 5 cut(s) 48, 311, 327, 620, 735
CviAII CATG 6 cut(s) 77, 83, 92, 353, 724, 784
DdeI CTNAG 3 cut(s) 342, 771, 818
DpnI GATC 2 cut(s) 75, 81
DpnII GATC 2 cut(s) 73, 79
Eam1104I CTCTTC 3 cut(s) 31, 168, 173
EarI CTCTTC 3 cut(s) 31, 168, 173
Ecl136II GAGCTC 2 cut(s) 721, 809
Eco24I GRGCYC 4 cut(s) 297, 657, 723, 811
Eco31I GGTCTC 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 48, 327
Eco53kI GAGCTC 2 cut(s) 721, 809
EcoICRI GAGCTC 2 cut(s) 721, 809
EcoO109I RGGNCCY 1 cut(s) 327
EcoRII CCWGG 2 cut(s) 231, 685
EcoT38I GRGCYC 4 cut(s) 297, 657, 723, 811
FaeI CATG 6 cut(s) 80, 86, 95, 356, 727, 787
FaiI YATR 9 cut(s) 78, 84, 93, 323, 354, 725, 729, 731, 785
FaqI GGGAC 2 cut(s) 211, 340
FatI CATG 6 cut(s) 76, 82, 91, 352, 723, 783
FbaI TGATCA 2 cut(s) 73, 79
Fnu4HI GCNGC 2 cut(s) 237, 240
FriOI GRGCYC 4 cut(s) 297, 657, 723, 811
Fsp4HI GCNGC 2 cut(s) 237, 240
FspBI CTAG 2 cut(s) 603, 762
GluI GCNGC 2 cut(s) 237, 240
GsaI CCCAGC 2 cut(s) 675, 708
GsuI CTGGAG 3 cut(s) 215, 353, 572
HaeIII GGCC 3 cut(s) 313, 622, 737
HapII CCGG 2 cut(s) 256, 392
Hin1II CATG 6 cut(s) 80, 86, 95, 356, 727, 787
HinfI GANTC 4 cut(s) 20, 199, 512, 767
HpaII CCGG 2 cut(s) 256, 392
HphI GGTGA 3 cut(s) 445, 710, 728
Hpy188I TCNGA 2 cut(s) 166, 772
Hpy188III TCNNGA 7 cut(s) 77, 83, 370, 400, 745, 762, 812
HpyAV CCTTC 6 cut(s) 216, 430, 481, 628, 637, 689
HpyCH4V TGCA 3 cut(s) 16, 358, 579
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 3 cut(s) 342, 771, 818
Hsp92II CATG 6 cut(s) 80, 86, 95, 356, 727, 787
KflI GGGWCCC 1 cut(s) 327
Ksp22I TGATCA 2 cut(s) 73, 79
Kzo9I GATC 2 cut(s) 73, 79
LmnI GCTCC 6 cut(s) 234, 258, 300, 556, 806, 814
Lsp1109I GCAGC 2 cut(s) 248, 251
MaeI CTAG 2 cut(s) 603, 762
MaeIII GTNAC 1 cut(s) 476
MalI GATC 2 cut(s) 75, 81
MboI GATC 2 cut(s) 73, 79
MhlI GDGCHC 4 cut(s) 297, 657, 723, 811
MluCI AATT 3 cut(s) 243, 382, 420
MlyI GAGTC 3 cut(s) 14, 193, 761
MmeI TCCRAC 4 cut(s) 85, 91, 97, 155
MseI TTAA 1 cut(s) 841
MslI CAYNNNNRTG 4 cut(s) 81, 96, 662, 728
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 2 cut(s) 256, 392
MspR9I CCNGG 3 cut(s) 233, 392, 687
MvaI CCWGG 2 cut(s) 233, 687
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 556
NciI CCSGG 1 cut(s) 392
NdeII GATC 2 cut(s) 73, 79
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 6 cut(s) 80, 86, 95, 356, 727, 787
NlaIV GGNNCC 6 cut(s) 254, 296, 312, 328, 329, 558
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 476
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 515
PagI TCATGA 2 cut(s) 76, 82
PfeI GAWTC 1 cut(s) 512
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 2 cut(s) 238, 241
PleI GAGTC 3 cut(s) 14, 193, 761
PpsI GAGTC 3 cut(s) 14, 193, 761
PpuMI RGGWCCY 1 cut(s) 327
Psp124BI GAGCTC 2 cut(s) 723, 811
Psp5II RGGWCCY 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 231, 685
PspFI CCCAGC 2 cut(s) 671, 704
PspGI CCWGG 2 cut(s) 231, 685
PspN4I GGNNCC 6 cut(s) 254, 296, 312, 328, 329, 558
PspPI GGNCC 5 cut(s) 48, 311, 327, 620, 735
PspPPI RGGWCCY 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 539
RseI CAYNNNNRTG 4 cut(s) 81, 96, 662, 728
SacI GAGCTC 2 cut(s) 723, 811
SaqAI TTAA 1 cut(s) 841
SatI GCNGC 2 cut(s) 237, 240
Sau3AI GATC 2 cut(s) 73, 79
Sau96I GGNCC 5 cut(s) 48, 311, 327, 620, 735
SchI GAGTC 3 cut(s) 14, 193, 761
ScrFI CCNGG 3 cut(s) 233, 392, 687
SduI GDGCHC 4 cut(s) 297, 657, 723, 811
SfcI CTRYAG 1 cut(s) 215
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 2 cut(s) 48, 327
SmiMI CAYNNNNRTG 4 cut(s) 81, 96, 662, 728
Sse9I AATT 3 cut(s) 243, 382, 420
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 603, 762
SstI GAGCTC 2 cut(s) 723, 811
StyD4I CCNGG 3 cut(s) 231, 390, 685
TaqI TCGA 6 cut(s) 248, 515, 521, 527, 744, 835
TasI AATT 3 cut(s) 243, 382, 420
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 841
Tru9I TTAA 1 cut(s) 841
TscAI CASTG 2 cut(s) 50, 306
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 2 cut(s) 236, 239
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 4 cut(s) 81, 108, 708, 803
TspRI CASTG 2 cut(s) 50, 306
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 48, 327
XapI RAATTY 1 cut(s) 382
XbaI TCTAGA 1 cut(s) 761
XceI RCATGY 1 cut(s) 356
XspI CTAG 2 cut(s) 603, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.