pycom06g18700

mizu-kussei

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0015885
Physical Location & Seq
Forward (+)
22345228 .. 22346088
861 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18700.1

Sequence Viewer

Length: 861 bp
ATGAGCATGGCAAGTAGCTTGCAGGACTCCTCCTCCAAGAGACACTTCCACTGGACAAACAAAGTTGGCAACGAAGAAGAAGATGAAGATGTTGATCATGTTGTTCTGCCGAGCCTCAACTCCTCCTCCTCCTCCTCCTCGTCGAAAACAAAATCCGATGAGGAGGAGAAGAAACAAGAGCATGATCATCATCAAAAACAACAGCATGGTCATGTGGCTCATCAACTTCCGAGGAGGAAACTGCAGGCAGCTGCGGTTTCCAGGATGAGGTCAGTCCTTAACGCTTTTGGTAAGAACCGATCCAGCCTACCACTCGGGCTTGGACCTCGAGTGGTAGGCACACTTTTCGGCTCCAGGCGTGGCCACGTACACTTTGCACTCCAGAGAGACCCAAGCTCACACCCGGCCTTCTTGATCGAGCTCGCCACCCCGATCAGCGGGTTGGTTAAAGAAATGGCATCCGGGCTCGTCAGAATTGCGTTGGAGTGTGACAAGGATCACAAAGACGATCATCAGAAAAAGAAAGATGAGAAAGCTCCGAGGTTGCTGGAAGAGCCAGTGTGGAGAACTTTTTGCAACGGTAAGAAATATGGTTTTGCTAGCAGAAGAGAATGTGGGGCCAAGGAGTGGAAGGTGTTGAAAGCTGTGGAGCCCATTTCAATGGGGGCTGGAGTGCTGCCGCCGTCTGCGGTGCTGGAAGGGAATGAAGCTGAAGCCGGGTCCGAATCCGACGGGGAACTAATGTACATGAGGGCTAAGTTTGAGAGAATTGTTGGGTCTAGAGACTCTGAGGCCTTCTACATGATGAACCCTGATAGCAATGGAGCTCCTGAGCTCAGTATCTACTTGCTCCGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

287

Amino Acids

31.81

Weight (kDa)

7.81

Isoelectric Point (pI)

62.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 111 - 285 8.1e-68 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23100
fragaria_vesca FvH4_5g12900
malus_domestica MD06G1209400.v1.1 MD14G1219900.v1.1
prunus_persica Prupe.5G214500_v2.0.a1
pyrus_communis pycom06g18700
rosa_chinensis RchiOBHm_Chr7g0184341
rosa_laevigata RLG00000004985
rosa_multiflora Rmu_sc0001557.1_g000024
rosa_roxburghii Rroxscaffold_3G00269710
rosa_samantha Rh7AG080900 Rh7BG066300 Rh7CG066600 Rh7DG066300
rosa_wichuraiana Rw7G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 627
AciI CCGC 4 cut(s) 254, 438, 680, 689
AclWI GGATC 2 cut(s) 294, 504
AcoI YGGCCR 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 2 cut(s) 369, 746
AfiI CCNNNNNNNGG 3 cut(s) 267, 437, 627
AgsI TTSAA 2 cut(s) 640, 660
AjnI CCWGG 2 cut(s) 260, 353
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 9 cut(s) 18, 251, 396, 421, 536, 644, 710, 827, 835
AluI AGCT 9 cut(s) 18, 251, 396, 421, 536, 644, 710, 827, 835
Alw21I GWGCWC 3 cut(s) 423, 829, 837
Alw26I GTCTC 3 cut(s) 34, 381, 777
AlwI GGATC 2 cut(s) 294, 504
Ama87I CYCGRG 2 cut(s) 314, 327
AoxI GGCC 4 cut(s) 361, 405, 618, 792
ApeKI GCWGC 3 cut(s) 248, 251, 676
AspS9I GGNCC 3 cut(s) 323, 618, 720
AsuC2I CCSGG 3 cut(s) 404, 463, 718
AsuNHI GCTAGC 1 cut(s) 599
AvaI CYCGRG 2 cut(s) 314, 327
AvaII GGWCC 2 cut(s) 323, 720
BalI TGGCCA 1 cut(s) 363
BanII GRGCYC 5 cut(s) 423, 468, 654, 829, 837
Bbv12I GWGCWC 3 cut(s) 423, 829, 837
BbvI GCAGC 3 cut(s) 238, 260, 663
BceAI ACGGC 1 cut(s) 667
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 2 cut(s) 262, 355
BclI TGATCA 2 cut(s) 94, 184
BcnI CCSGG 3 cut(s) 404, 463, 718
BcoDI GTCTC 3 cut(s) 34, 381, 777
BfaI CTAG 2 cut(s) 600, 780
BfmI CTRYAG 1 cut(s) 242
BisI GCNGC 4 cut(s) 249, 252, 677, 680
BlsI GCNGC 4 cut(s) 250, 253, 678, 681
Bme1390I CCNGG 5 cut(s) 262, 355, 404, 463, 718
Bme18I GGWCC 2 cut(s) 323, 720
BmeT110I CYCGRG 2 cut(s) 314, 327
BmgT120I GGNCC 3 cut(s) 323, 618, 720
BmiI GGNNCC 4 cut(s) 352, 619, 651, 721
BmrFI CCNGG 5 cut(s) 262, 355, 404, 463, 718
BmsI GCATC 1 cut(s) 467
BmtI GCTAGC 1 cut(s) 603
BpmI CTGGAG 3 cut(s) 337, 365, 690
Bpu10I CCTNAGC 1 cut(s) 831
BpuMI CCSGG 3 cut(s) 404, 463, 718
BsaAI YACGTR 1 cut(s) 367
BsaBI GATNNNNATC 2 cut(s) 93, 189
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 230, 539, 621
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 110, 140
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 437, 627
Bse1I ACTGG 2 cut(s) 56, 557
Bse3DI GCAATG 1 cut(s) 826
Bse8I GATNNNNATC 2 cut(s) 93, 189
BseBI CCWGG 2 cut(s) 262, 355
BseDI CCNNGG 3 cut(s) 230, 539, 621
BseGI GGATG 2 cut(s) 270, 458
BseJI GATNNNNATC 2 cut(s) 93, 189
BseLI CCNNNNNNNGG 3 cut(s) 267, 437, 627
BseMI GCAATG 1 cut(s) 826
BseMII CTCAG 3 cut(s) 780, 822, 850
BseNI ACTGG 2 cut(s) 56, 557
BseXI GCAGC 3 cut(s) 238, 260, 663
BshFI GGCC 4 cut(s) 363, 407, 620, 794
BsiHKAI GWGCWC 3 cut(s) 423, 829, 837
BsiHKCI CYCGRG 2 cut(s) 314, 327
BsiSI CCGG 3 cut(s) 404, 462, 717
BslI CCNNNNNNNGG 3 cut(s) 267, 437, 627
BsmAI GTCTC 3 cut(s) 34, 381, 777
BsnI GGCC 4 cut(s) 363, 407, 620, 794
Bso31I GGTCTC 1 cut(s) 381
BsoBI CYCGRG 2 cut(s) 314, 327
Bsp1286I GDGCHC 5 cut(s) 423, 468, 654, 829, 837
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
BspACI CCGC 4 cut(s) 254, 438, 680, 689
BspANI GGCC 4 cut(s) 363, 407, 620, 794
BspCNI CTCAG 3 cut(s) 781, 823, 849
BspLI GGNNCC 4 cut(s) 352, 619, 651, 721
BspMAI CTGCAG 1 cut(s) 246
BspOI GCTAGC 1 cut(s) 603
BspPI GGATC 2 cut(s) 294, 504
BspQI GCTCTTC 1 cut(s) 546
BspTNI GGTCTC 1 cut(s) 381
BsrDI GCAATG 1 cut(s) 826
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 2 cut(s) 56, 557
BssECI CCNNGG 3 cut(s) 230, 539, 621
BssMI GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
BssT1I CCWWGG 1 cut(s) 621
Bst2UI CCWGG 2 cut(s) 262, 355
Bst4CI ACNGT 1 cut(s) 581
Bst6I CTCTTC 2 cut(s) 546, 601
BstAUI TGTACA 1 cut(s) 744
BstBAI YACGTR 1 cut(s) 367
BstC8I GCNNGC 4 cut(s) 20, 246, 423, 601
BstDEI CTNAG 4 cut(s) 756, 789, 831, 836
BstF5I GGATG 2 cut(s) 270, 458
BstKTI GATC 7 cut(s) 97, 187, 302, 417, 435, 499, 511
BstMAI GTCTC 3 cut(s) 34, 381, 777
BstMBI GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
BstMWI GCNNNNNNNGC 1 cut(s) 553
BstNI CCWGG 2 cut(s) 262, 355
BstSCI CCNGG 5 cut(s) 260, 353, 402, 461, 716
BstSFI CTRYAG 1 cut(s) 242
BstV1I GCAGC 3 cut(s) 238, 260, 663
BstXI CCANNNNNNTGG 1 cut(s) 661
BsuRI GGCC 4 cut(s) 363, 407, 620, 794
BtsCI GGATG 2 cut(s) 270, 458
BtsIMutI CAGTG 2 cut(s) 49, 564
Cac8I GCNNGC 4 cut(s) 20, 246, 423, 601
Cfr13I GGNCC 3 cut(s) 323, 618, 720
Csp6I GTAC 2 cut(s) 368, 745
CviAII CATG 7 cut(s) 7, 98, 182, 206, 212, 748, 802
CviQI GTAC 2 cut(s) 368, 745
DdeI CTNAG 4 cut(s) 756, 789, 831, 836
DpnI GATC 7 cut(s) 96, 186, 301, 416, 434, 498, 510
DpnII GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 2 cut(s) 546, 601
EarI CTCTTC 2 cut(s) 546, 601
Ecl136II GAGCTC 3 cut(s) 421, 827, 835
Eco130I CCWWGG 1 cut(s) 621
Eco147I AGGCCT 1 cut(s) 794
Eco24I GRGCYC 5 cut(s) 423, 468, 654, 829, 837
Eco31I GGTCTC 1 cut(s) 381
Eco47I GGWCC 2 cut(s) 323, 720
Eco53kI GAGCTC 3 cut(s) 421, 827, 835
Eco57I CTGAAG 1 cut(s) 732
Eco88I CYCGRG 2 cut(s) 314, 327
EcoICRI GAGCTC 3 cut(s) 421, 827, 835
EcoRII CCWGG 2 cut(s) 260, 353
EcoT14I CCWWGG 1 cut(s) 621
EcoT38I GRGCYC 5 cut(s) 423, 468, 654, 829, 837
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 7 cut(s) 10, 101, 185, 209, 215, 751, 805
FaiI YATR 8 cut(s) 8, 99, 183, 207, 213, 591, 749, 803
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FatI CATG 7 cut(s) 6, 97, 181, 205, 211, 747, 801
FauI CCCGC 1 cut(s) 431
FbaI TGATCA 2 cut(s) 94, 184
Fnu4HI GCNGC 4 cut(s) 249, 252, 677, 680
FokI GGATG 2 cut(s) 277, 445
FriOI GRGCYC 5 cut(s) 423, 468, 654, 829, 837
Fsp4HI GCNGC 4 cut(s) 249, 252, 677, 680
FspBI CTAG 2 cut(s) 600, 780
GluI GCNGC 4 cut(s) 249, 252, 677, 680
GsuI CTGGAG 3 cut(s) 337, 365, 690
HaeIII GGCC 4 cut(s) 363, 407, 620, 794
HapII CCGG 3 cut(s) 404, 462, 717
Hin1II CATG 7 cut(s) 10, 101, 185, 209, 215, 751, 805
HinfI GANTC 4 cut(s) 26, 725, 785, 855
HpaII CCGG 3 cut(s) 404, 462, 717
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 9 cut(s) 157, 231, 473, 516, 540, 724, 730, 790, 854
Hpy188III TCNNGA 4 cut(s) 382, 412, 780, 830
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 2 cut(s) 145, 734
HpyAV CCTTC 4 cut(s) 418, 625, 692, 805
HpyCH4III ACNGT 1 cut(s) 581
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 22, 244, 377, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 553
HpyF3I CTNAG 4 cut(s) 756, 789, 831, 836
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 7 cut(s) 10, 101, 185, 209, 215, 751, 805
Ksp22I TGATCA 2 cut(s) 94, 184
Kzo9I GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 6 cut(s) 356, 541, 649, 824, 832, 855
Lsp1109I GCAGC 3 cut(s) 238, 260, 663
LweI GCATC 1 cut(s) 467
MaeI CTAG 2 cut(s) 600, 780
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 1 cut(s) 488
MalI GATC 7 cut(s) 96, 186, 301, 416, 434, 498, 510
MboI GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
MboII GAAGA 7 cut(s) 86, 89, 92, 98, 181, 563, 618
MhlI GDGCHC 5 cut(s) 423, 468, 654, 829, 837
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 2 cut(s) 474, 768
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 2 cut(s) 20, 779
MmeI TCCRAC 2 cut(s) 462, 753
Mox20I TGGCCA 1 cut(s) 363
MscI TGGCCA 1 cut(s) 363
MseI TTAA 2 cut(s) 279, 447
MslI CAYNNNNRTG 2 cut(s) 210, 659
Msp20I TGGCCA 1 cut(s) 363
MspA1I CMGCKG 2 cut(s) 251, 438
MspI CCGG 3 cut(s) 404, 462, 717
MspR9I CCNGG 5 cut(s) 262, 355, 404, 463, 718
MvaI CCWGG 2 cut(s) 262, 355
MwoI GCNNNNNNNGC 1 cut(s) 553
NciI CCSGG 3 cut(s) 404, 463, 718
NdeII GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 7 cut(s) 10, 101, 185, 209, 215, 751, 805
NlaIV GGNNCC 4 cut(s) 352, 619, 651, 721
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 488
PaeR7I CTCGAG 1 cut(s) 327
PceI AGGCCT 1 cut(s) 794
PciSI GCTCTTC 1 cut(s) 546
PfeI GAWTC 1 cut(s) 725
PflMI CCANNNNNTGG 1 cut(s) 627
PfoI TCCNGGA 1 cut(s) 260
PkrI GCNGC 4 cut(s) 250, 253, 678, 681
PleI GAGTC 2 cut(s) 20, 779
PpsI GAGTC 2 cut(s) 20, 779
Ppu21I YACGTR 1 cut(s) 367
Psp124BI GAGCTC 3 cut(s) 423, 829, 837
Psp6I CCWGG 2 cut(s) 260, 353
PspGI CCWGG 2 cut(s) 260, 353
PspN4I GGNNCC 4 cut(s) 352, 619, 651, 721
PspPI GGNCC 3 cut(s) 323, 618, 720
PspXI VCTCGAGB 1 cut(s) 327
PstI CTGCAG 1 cut(s) 246
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 2 cut(s) 369, 746
RsaNI GTAC 2 cut(s) 368, 745
RseI CAYNNNNRTG 2 cut(s) 210, 659
SacI GAGCTC 3 cut(s) 423, 829, 837
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 2 cut(s) 279, 447
SatI GCNGC 4 cut(s) 249, 252, 677, 680
Sau3AI GATC 7 cut(s) 94, 184, 299, 414, 432, 496, 508
Sau96I GGNCC 3 cut(s) 323, 618, 720
SchI GAGTC 2 cut(s) 20, 779
ScrFI CCNGG 5 cut(s) 262, 355, 404, 463, 718
SduI GDGCHC 5 cut(s) 423, 468, 654, 829, 837
SfaNI GCATC 1 cut(s) 467
SfcI CTRYAG 1 cut(s) 242
Sfr274I CTCGAG 1 cut(s) 327
SinI GGWCC 2 cut(s) 323, 720
SlaI CTCGAG 1 cut(s) 327
SmiMI CAYNNNNRTG 2 cut(s) 210, 659
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 2 cut(s) 474, 768
SseBI AGGCCT 1 cut(s) 794
SsiI CCGC 4 cut(s) 254, 438, 680, 689
SspMI CTAG 2 cut(s) 600, 780
SstI GAGCTC 3 cut(s) 423, 829, 837
StuI AGGCCT 1 cut(s) 794
StyD4I CCNGG 5 cut(s) 260, 353, 402, 461, 716
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 1 cut(s) 581
TaiI ACGT 1 cut(s) 369
TaqI TCGA 3 cut(s) 143, 328, 417
TasI AATT 2 cut(s) 474, 768
TatI WGTACW 1 cut(s) 744
TauI GCSGC 1 cut(s) 682
TfiI GAWTC 1 cut(s) 725
Tru1I TTAA 2 cut(s) 279, 447
Tru9I TTAA 2 cut(s) 279, 447
TscAI CASTG 2 cut(s) 56, 564
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 3 cut(s) 248, 251, 676
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 3 cut(s) 99, 720, 821
TspRI CASTG 2 cut(s) 56, 564
Van91I CCANNNNNTGG 1 cut(s) 627
VpaK11BI GGWCC 2 cut(s) 323, 720
XbaI TCTAGA 1 cut(s) 779
XhoI CTCGAG 1 cut(s) 327
XspI CTAG 2 cut(s) 600, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.