MD14G1219900.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015885
Physical Location & Seq
Forward (+)
30215427 .. 30216340
914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1219900.v1.1.491

Sequence Viewer

Length: 864 bp
ATGAAAGCAGTGAGCATGGCAAGGAGCTTGCAGGACTCCTCCTCCAAGAGACACTTCCTCTGGACAAACAAAGTTGGCAACGAAGAAGAAGAAGAGAATGATGTTGATAATGTAGTTCTTCCGAGCCTCAAATCCTCCTCCGCGTCGAAAGCAAAATCCGACGAGGAGGAGAAGAAACAAGAGCATGATCATCATCAAAAACAACAGCATGGTCATGAGGCTCATCAACTTCCCAGGAGGAAACTGCAAGCGGCTGCAGTTTCTAGGCTGCGGTCTGTTCTTACCACATTTGGTAAGAACCGATCTAGCCTACCACTCGGGCTGGGACCCCGAGTGGTAGGCACTCTTTTCGGCTCCAGGCGTGGCCACGTACATTTTGCACTCCAGAGAGACCCTAGCTCACATCCGGCCTTCTTGATCGAGCTTGCAACGCCGATCAGCGGGTTGGTTAAAGAAATGGCGTCCGGCCTCGTCAGAATTGCGTTGGAGTGTGACAAGGATCACAAAGAAGATCTTCAAAAGAAGAAAGATCAGAAATCTCTGAGGCTGCTGGAAGAGCCAGTGTGGAGAACTTTTTGCAACGGTAAGAAATATGGTTTTGCTAGCAAAAGAGAATGTGGGGCCAAGGAGTGGAAGGTGTTGAAAGCTGTGGAGCCCATTTCGATGGGGGCTGGTGTGCTGCCGCCGTCTGCGGCGGTGGAAGGGAATGAAGCTGAAGCCGGGTCCGAATCCGACGGCGAACTCATGTACATGAGGGCCAAGTTTGAGAGAATTGTTGGGTCTAGAGACTCTGAGGCCTTCTACATGATGAACCCTGATAGCAATGGAGCTCCTGAGCTCAGTATCTACTTGCTCCGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

288

Amino Acids

31.94

Weight (kDa)

8.83

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 112 - 286 1e-67 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23100
fragaria_vesca FvH4_5g12900
malus_domestica MD06G1209400.v1.1 MD14G1219900.v1.1
prunus_persica Prupe.5G214500_v2.0.a1
pyrus_communis pycom06g18700
rosa_chinensis RchiOBHm_Chr7g0184341
rosa_laevigata RLG00000004985
rosa_multiflora Rmu_sc0001557.1_g000024
rosa_roxburghii Rroxscaffold_3G00269710
rosa_samantha Rh7AG080900 Rh7BG066300 Rh7CG066600 Rh7DG066300
rosa_wichuraiana Rw7G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 630
AccII CGCG 1 cut(s) 143
AciI CCGC 7 cut(s) 141, 251, 271, 441, 683, 692, 695
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 735
AcyI GRCGYC 1 cut(s) 461
AfaI GTAC 2 cut(s) 372, 749
AfiI CCNNNNNNNGG 2 cut(s) 440, 630
AgsI TTSAA 2 cut(s) 518, 643
AjnI CCWGG 2 cut(s) 233, 356
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
AluBI AGCT 7 cut(s) 27, 399, 424, 647, 713, 830, 838
AluI AGCT 7 cut(s) 27, 399, 424, 647, 713, 830, 838
Alw21I GWGCWC 2 cut(s) 832, 840
Alw26I GTCTC 3 cut(s) 43, 384, 780
AlwI GGATC 1 cut(s) 507
Ama87I CYCGRG 2 cut(s) 317, 330
AoxI GGCC 6 cut(s) 364, 408, 466, 621, 756, 795
ApeKI GCWGC 4 cut(s) 254, 268, 547, 679
Asp700I GAANNNNTTC 1 cut(s) 513
AspS9I GGNCC 4 cut(s) 326, 621, 723, 756
AsuC2I CCSGG 1 cut(s) 721
AsuNHI GCTAGC 1 cut(s) 602
AvaI CYCGRG 2 cut(s) 317, 330
AvaII GGWCC 2 cut(s) 326, 723
BalI TGGCCA 1 cut(s) 366
BanII GRGCYC 3 cut(s) 657, 832, 840
Bbv12I GWGCWC 2 cut(s) 832, 840
BbvI GCAGC 4 cut(s) 241, 255, 534, 666
BccI CCATC 1 cut(s) 658
BceAI ACGGC 2 cut(s) 670, 751
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BciT130I CCWGG 2 cut(s) 235, 358
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 1 cut(s) 721
BcoDI GTCTC 3 cut(s) 43, 384, 780
BfaI CTAG 5 cut(s) 264, 306, 396, 603, 783
BfmI CTRYAG 1 cut(s) 255
BglII AGATCT 1 cut(s) 511
BisI GCNGC 7 cut(s) 252, 255, 269, 548, 680, 683, 693
BlsI GCNGC 7 cut(s) 253, 256, 270, 549, 681, 684, 694
Bme1390I CCNGG 3 cut(s) 235, 358, 721
Bme18I GGWCC 2 cut(s) 326, 723
BmeT110I CYCGRG 2 cut(s) 317, 330
BmgT120I GGNCC 4 cut(s) 326, 621, 723, 756
BmiI GGNNCC 6 cut(s) 327, 328, 355, 622, 654, 724
BmrFI CCNGG 3 cut(s) 235, 358, 721
BmtI GCTAGC 1 cut(s) 606
BpmI CTGGAG 2 cut(s) 340, 368
Bpu10I CCTNAGC 1 cut(s) 834
BpuMI CCSGG 1 cut(s) 721
BsaAI YACGTR 1 cut(s) 370
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 461
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 2 cut(s) 233, 624
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 630
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 1 cut(s) 829
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 2 cut(s) 235, 358
BseDI CCNNGG 2 cut(s) 233, 624
BseGI GGATG 1 cut(s) 403
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 440, 630
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 4 cut(s) 533, 783, 825, 853
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 5 cut(s) 28, 31, 127, 179, 182
BseXI GCAGC 4 cut(s) 241, 255, 534, 666
BseYI CCCAGC 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 6 cut(s) 366, 410, 468, 623, 758, 797
BsiHKAI GWGCWC 2 cut(s) 832, 840
BsiHKCI CYCGRG 2 cut(s) 317, 330
BsiSI CCGG 3 cut(s) 407, 465, 720
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 2 cut(s) 440, 630
BsmAI GTCTC 3 cut(s) 43, 384, 780
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 6 cut(s) 366, 410, 468, 623, 758, 797
Bso31I GGTCTC 1 cut(s) 384
BsoBI CYCGRG 2 cut(s) 317, 330
Bsp1286I GDGCHC 3 cut(s) 657, 832, 840
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
BspACI CCGC 7 cut(s) 141, 251, 271, 441, 683, 692, 695
BspANI GGCC 6 cut(s) 366, 410, 468, 623, 758, 797
BspCNI CTCAG 4 cut(s) 534, 784, 826, 852
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 214
BspLI GGNNCC 6 cut(s) 327, 328, 355, 622, 654, 724
BspMAI CTGCAG 1 cut(s) 259
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 1 cut(s) 507
BspQI GCTCTTC 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 829
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 2 cut(s) 233, 624
BssMI GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
BssNI GRCGYC 1 cut(s) 461
BssT1I CCWWGG 1 cut(s) 624
Bst2UI CCWGG 2 cut(s) 235, 358
Bst4CI ACNGT 1 cut(s) 584
Bst6I CTCTTC 2 cut(s) 87, 549
BstACI GRCGYC 1 cut(s) 461
BstAUI TGTACA 1 cut(s) 747
BstBAI YACGTR 1 cut(s) 370
BstC8I GCNNGC 4 cut(s) 29, 249, 426, 604
BstDEI CTNAG 4 cut(s) 542, 792, 834, 839
BstF5I GGATG 1 cut(s) 403
BstFNI CGCG 1 cut(s) 143
BstKTI GATC 7 cut(s) 190, 305, 420, 438, 502, 514, 532
BstMAI GTCTC 3 cut(s) 43, 384, 780
BstMBI GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
BstMWI GCNNNNNNNGC 3 cut(s) 149, 430, 556
BstNI CCWGG 2 cut(s) 235, 358
BstSCI CCNGG 3 cut(s) 233, 356, 719
BstSFI CTRYAG 1 cut(s) 255
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 241, 255, 534, 666
BstX2I RGATCY 1 cut(s) 511
BstXI CCANNNNNNTGG 1 cut(s) 664
BstYI RGATCY 1 cut(s) 511
BsuRI GGCC 6 cut(s) 366, 410, 468, 623, 758, 797
BtsCI GGATG 1 cut(s) 403
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 15, 567
Cac8I GCNNGC 4 cut(s) 29, 249, 426, 604
CciI TCATGA 1 cut(s) 214
Cfr13I GGNCC 4 cut(s) 326, 621, 723, 756
CseI GACGC 2 cut(s) 132, 450
Csp6I GTAC 2 cut(s) 371, 748
CviAII CATG 7 cut(s) 16, 185, 209, 215, 745, 751, 805
CviQI GTAC 2 cut(s) 371, 748
DdeI CTNAG 4 cut(s) 542, 792, 834, 839
DpnI GATC 7 cut(s) 189, 304, 419, 437, 501, 513, 531
DpnII GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 87, 549
EarI CTCTTC 2 cut(s) 87, 549
Ecl136II GAGCTC 2 cut(s) 830, 838
Eco130I CCWWGG 1 cut(s) 624
Eco147I AGGCCT 1 cut(s) 797
Eco24I GRGCYC 3 cut(s) 657, 832, 840
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 2 cut(s) 326, 723
Eco53kI GAGCTC 2 cut(s) 830, 838
Eco57I CTGAAG 1 cut(s) 735
Eco88I CYCGRG 2 cut(s) 317, 330
EcoICRI GAGCTC 2 cut(s) 830, 838
EcoO109I RGGNCCY 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 233, 356
EcoT14I CCWWGG 1 cut(s) 624
EcoT38I GRGCYC 3 cut(s) 657, 832, 840
ErhI CCWWGG 1 cut(s) 624
FaeI CATG 7 cut(s) 19, 188, 212, 218, 748, 754, 808
FaiI YATR 8 cut(s) 17, 186, 210, 216, 594, 746, 752, 806
FalI AAGNNNNNCTT 4 cut(s) 38, 70, 498, 530
FaqI GGGAC 1 cut(s) 339
FatI CATG 7 cut(s) 15, 184, 208, 214, 744, 750, 804
FauI CCCGC 1 cut(s) 434
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 7 cut(s) 252, 255, 269, 548, 680, 683, 693
FokI GGATG 1 cut(s) 390
FriOI GRGCYC 3 cut(s) 657, 832, 840
Fsp4HI GCNGC 7 cut(s) 252, 255, 269, 548, 680, 683, 693
FspBI CTAG 5 cut(s) 264, 306, 396, 603, 783
GluI GCNGC 7 cut(s) 252, 255, 269, 548, 680, 683, 693
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 2 cut(s) 340, 368
HaeIII GGCC 6 cut(s) 366, 410, 468, 623, 758, 797
HapII CCGG 3 cut(s) 407, 465, 720
HgaI GACGC 2 cut(s) 132, 450
Hin1I GRCGYC 1 cut(s) 461
Hin1II CATG 7 cut(s) 19, 188, 212, 218, 748, 754, 808
HinfI GANTC 4 cut(s) 35, 728, 788, 858
HpaII CCGG 3 cut(s) 407, 465, 720
Hpy188I TCNGA 9 cut(s) 123, 160, 476, 534, 543, 727, 733, 793, 857
Hpy188III TCNNGA 6 cut(s) 61, 215, 385, 415, 783, 833
Hpy99I CGWCG 3 cut(s) 148, 164, 737
HpyAV CCTTC 4 cut(s) 421, 628, 695, 808
HpyCH4III ACNGT 1 cut(s) 584
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 6 cut(s) 31, 247, 257, 380, 428, 579
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 430, 556
HpyF3I CTNAG 4 cut(s) 542, 792, 834, 839
HpySE526I ACGT 1 cut(s) 369
Hsp92I GRCGYC 1 cut(s) 461
Hsp92II CATG 7 cut(s) 19, 188, 212, 218, 748, 754, 808
KflI GGGWCCC 1 cut(s) 326
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 6 cut(s) 24, 359, 652, 827, 835, 858
Lsp1109I GCAGC 4 cut(s) 241, 255, 534, 666
MaeI CTAG 5 cut(s) 264, 306, 396, 603, 783
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 491
MalI GATC 7 cut(s) 189, 304, 419, 437, 501, 513, 531
MboI GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 3 cut(s) 657, 832, 840
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 2 cut(s) 477, 771
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 2 cut(s) 29, 782
MmeI TCCRAC 3 cut(s) 183, 465, 756
Mox20I TGGCCA 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 513
MscI TGGCCA 1 cut(s) 366
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 3 cut(s) 213, 662, 749
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 441
MspI CCGG 3 cut(s) 407, 465, 720
MspR9I CCNGG 3 cut(s) 235, 358, 721
MvaI CCWGG 2 cut(s) 235, 358
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 149, 430, 556
NciI CCSGG 1 cut(s) 721
NdeII GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 7 cut(s) 19, 188, 212, 218, 748, 754, 808
NlaIV GGNNCC 6 cut(s) 327, 328, 355, 622, 654, 724
NmuCI GTSAC 1 cut(s) 491
PagI TCATGA 1 cut(s) 214
PceI AGGCCT 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 728
PflMI CCANNNNNTGG 1 cut(s) 630
PkrI GCNGC 7 cut(s) 253, 256, 270, 549, 681, 684, 694
PleI GAGTC 2 cut(s) 29, 782
PpsI GAGTC 2 cut(s) 29, 782
Ppu21I YACGTR 1 cut(s) 370
PpuMI RGGWCCY 1 cut(s) 326
Psp124BI GAGCTC 2 cut(s) 832, 840
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 233, 356
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 2 cut(s) 233, 356
PspN4I GGNNCC 6 cut(s) 327, 328, 355, 622, 654, 724
PspPI GGNCC 4 cut(s) 326, 621, 723, 756
PspPPI RGGWCCY 1 cut(s) 326
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 511
RsaI GTAC 2 cut(s) 372, 749
RsaNI GTAC 2 cut(s) 371, 748
RseI CAYNNNNRTG 3 cut(s) 213, 662, 749
SacI GAGCTC 2 cut(s) 832, 840
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 7 cut(s) 252, 255, 269, 548, 680, 683, 693
Sau3AI GATC 7 cut(s) 187, 302, 417, 435, 499, 511, 529
Sau96I GGNCC 4 cut(s) 326, 621, 723, 756
SchI GAGTC 2 cut(s) 29, 782
ScrFI CCNGG 3 cut(s) 235, 358, 721
SduI GDGCHC 3 cut(s) 657, 832, 840
SetI ASST 9 cut(s) 29, 372, 401, 426, 639, 649, 715, 832, 840
SfcI CTRYAG 1 cut(s) 255
SinI GGWCC 2 cut(s) 326, 723
SmiMI CAYNNNNRTG 3 cut(s) 213, 662, 749
Sse9I AATT 2 cut(s) 477, 771
SseBI AGGCCT 1 cut(s) 797
SsiI CCGC 7 cut(s) 141, 251, 271, 441, 683, 692, 695
SspMI CTAG 5 cut(s) 264, 306, 396, 603, 783
SstI GAGCTC 2 cut(s) 832, 840
StuI AGGCCT 1 cut(s) 797
StyD4I CCNGG 3 cut(s) 233, 356, 719
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 1 cut(s) 584
TaiI ACGT 1 cut(s) 372
TaqI TCGA 3 cut(s) 146, 420, 662
TasI AATT 2 cut(s) 477, 771
TatI WGTACW 1 cut(s) 747
TauI GCSGC 3 cut(s) 254, 685, 695
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 2 cut(s) 15, 567
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 4 cut(s) 254, 268, 547, 679
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 3 cut(s) 17, 723, 824
TspRI CASTG 2 cut(s) 15, 567
Van91I CCANNNNNTGG 1 cut(s) 630
VpaK11BI GGWCC 2 cut(s) 326, 723
XbaI TCTAGA 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 5 cut(s) 264, 306, 396, 603, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.