Rh7BG066300

mizu-kussei

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0015885
Physical Location & Seq
Forward (+)
4404198 .. 4405028
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG066300.1

Sequence Viewer

Length: 831 bp
ATGGCAAAGAGCTTGCAGGACTCCTCTTCCAAAAGACACTTCCACTGGACCAACAAAGTTGGCAATGAAGATGATCATGATCATGATAACCATGAAGATGGAGTTGGAGTAGGTCTTCCAACCTTGAAATATTCTCTGTCAAAAGCAGTTTCTGATGAGGAAGAGAAGAGAAAGGAGGGTCAAGCGACTCAAAAGGTTCAGCTACAGAGAAGGAAGCTCCAGGCAGCAGCAATTTCGAGGCTCCGGTCGGTGCTGACCGCGTTTGGGAAGAACAGGTCAGGGCTCCCACTGGGTCTGGGGCCGAGAGTTATAGGGACCCTTTTTGGGTCTAAGAAAGGGCATGTGCATTTTGCTCTCCAGAGAGACCCAAATTCACACCCGGCTTTCCTGATTGAGCTTGCTACTCCAATTAGTGGGCTGGTGAAGGAAATGGCTTCTGGGCTTGTGAGGATTGCTTTGGAGTGTGACAAGGAGAAGCAGGAGGAGAAGAAAACAGAGGATTCGAAATCGAAGTCGAAAAGTTCAGCTGTGAGGTTGCTGGAGGAGCCTGTGTGGAGGACTTACTGCAATGGCAAGAAGTCTGGGTTTGCTAGCAGGAGGGAATGTGGGCCCAAAGAGTGGAAGGTGTTGAAGGCTGTTGAGCCCATTTCAATGGGTGCCGGGGTCTTGCCTCCTGGGAATGAAGGTGAAGCTGGGTGTGATGGTGAGCTCATGTATATGAGGGCCAAGTTTGAGAGGGTTGTTGGGTCCAGAGACTCTGAGGCTTTCTACATGATGAACCCAGATAGCAATGGAGCTCCTGAACTCAGTATCTACTTGCTTCGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

276

Amino Acids

30.49

Weight (kDa)

9.13

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 103 - 275 1.6e-68 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23100
fragaria_vesca FvH4_5g12900
malus_domestica MD06G1209400.v1.1 MD14G1219900.v1.1
prunus_persica Prupe.5G214500_v2.0.a1
pyrus_communis pycom06g18700
rosa_chinensis RchiOBHm_Chr7g0184341
rosa_laevigata RLG00000004985
rosa_multiflora Rmu_sc0001557.1_g000024
rosa_roxburghii Rroxscaffold_3G00269710
rosa_samantha Rh7AG080900 Rh7BG066300 Rh7CG066600 Rh7DG066300
rosa_wichuraiana Rw7G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 656
AccB7I CCANNNNNTGG 2 cut(s) 413, 618
AccII CGCG 1 cut(s) 260
AciI CCGC 1 cut(s) 258
AcsI RAATTY 1 cut(s) 370
AfiI CCNNNNNNNGG 4 cut(s) 264, 324, 413, 618
AgsI TTSAA 3 cut(s) 127, 631, 651
AjnI CCWGG 2 cut(s) 219, 673
AjuI GAANNNNNNNTTGG 4 cut(s) 23, 55, 87, 119
AluBI AGCT 8 cut(s) 12, 202, 217, 397, 527, 692, 709, 797
AluI AGCT 8 cut(s) 12, 202, 217, 397, 527, 692, 709, 797
Alw21I GWGCWC 2 cut(s) 711, 799
Alw26I GTCTC 2 cut(s) 357, 747
AlwNI CAGNNNCTG 1 cut(s) 152
AoxI GGCC 3 cut(s) 299, 608, 723
ApaI GGGCCC 1 cut(s) 612
ApeKI GCWGC 2 cut(s) 224, 227
ApoI RAATTY 1 cut(s) 370
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
AspS9I GGNCC 7 cut(s) 48, 299, 315, 608, 609, 723, 747
AsuC2I CCSGG 2 cut(s) 380, 661
AsuHPI GGTGA 3 cut(s) 433, 698, 716
AsuII TTCGAA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 590
AvaII GGWCC 3 cut(s) 48, 315, 747
BaeGI GKGCMC 1 cut(s) 612
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 5 cut(s) 285, 612, 645, 711, 799
BbsI GAAGAC 1 cut(s) 107
Bbv12I GWGCWC 2 cut(s) 711, 799
BbvI GCAGC 2 cut(s) 236, 239
BccI CCATC 2 cut(s) 92, 695
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 2 cut(s) 221, 675
BclI TGATCA 2 cut(s) 73, 79
BcnI CCSGG 2 cut(s) 380, 661
BcoDI GTCTC 2 cut(s) 357, 747
BfaI CTAG 1 cut(s) 591
BfmI CTRYAG 1 cut(s) 203
BisI GCNGC 2 cut(s) 225, 228
BlsI GCNGC 2 cut(s) 226, 229
Bme1390I CCNGG 4 cut(s) 221, 380, 661, 675
Bme18I GGWCC 3 cut(s) 48, 315, 747
BmgT120I GGNCC 7 cut(s) 48, 299, 315, 608, 609, 723, 747
BmiI GGNNCC 9 cut(s) 242, 284, 300, 316, 317, 546, 610, 658, 748
BmrFI CCNGG 4 cut(s) 221, 380, 661, 675
BmrI ACTGGG 1 cut(s) 299
BmtI GCTAGC 1 cut(s) 594
BmuI ACTGGG 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 107
BpmI CTGGAG 3 cut(s) 203, 341, 560
Bpu14I TTCGAA 1 cut(s) 503
BpuMI CCSGG 2 cut(s) 380, 661
BsaBI GATNNNNATC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 660, 674
BsaWI WCCGGW 1 cut(s) 243
BsaXI ACNNNNNCTCC 6 cut(s) 93, 123, 536, 566, 589, 619
Bsc4I CCNNNNNNNGG 4 cut(s) 264, 324, 413, 618
Bse1I ACTGG 2 cut(s) 50, 294
Bse3DI GCAATG 3 cut(s) 70, 574, 796
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 2 cut(s) 221, 675
BseDI CCNNGG 2 cut(s) 660, 674
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 4 cut(s) 264, 324, 413, 618
BseMI GCAATG 3 cut(s) 70, 574, 796
BseMII CTCAG 2 cut(s) 750, 820
BseNI ACTGG 2 cut(s) 50, 294
BseRI GAGGAG 3 cut(s) 13, 497, 557
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 2 cut(s) 236, 239
BseYI CCCAGC 1 cut(s) 692
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 1 cut(s) 248
BshFI GGCC 3 cut(s) 301, 610, 725
BshNI GGYRCC 1 cut(s) 656
BsiEI CGRYCG 1 cut(s) 248
BsiHKAI GWGCWC 2 cut(s) 711, 799
BsiSI CCGG 3 cut(s) 244, 380, 660
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 4 cut(s) 264, 324, 413, 618
BsmAI GTCTC 2 cut(s) 357, 747
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 3 cut(s) 301, 610, 725
Bso31I GGTCTC 1 cut(s) 357
Bsp119I TTCGAA 1 cut(s) 503
Bsp120I GGGCCC 1 cut(s) 608
Bsp1286I GDGCHC 5 cut(s) 285, 612, 645, 711, 799
Bsp143I GATC 2 cut(s) 73, 79
BspACI CCGC 1 cut(s) 258
BspANI GGCC 3 cut(s) 301, 610, 725
BspCNI CTCAG 2 cut(s) 751, 819
BspFNI CGCG 1 cut(s) 260
BspHI TCATGA 2 cut(s) 76, 82
BspLI GGNNCC 9 cut(s) 242, 284, 300, 316, 317, 546, 610, 658, 748
BspOI GCTAGC 1 cut(s) 594
BspT104I TTCGAA 1 cut(s) 503
BspT107I GGYRCC 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 357
BsrDI GCAATG 3 cut(s) 70, 574, 796
BsrI ACTGG 2 cut(s) 50, 294
BssECI CCNNGG 2 cut(s) 660, 674
BssMI GATC 2 cut(s) 73, 79
Bst2UI CCWGG 2 cut(s) 221, 675
Bst6I CTCTTC 3 cut(s) 31, 156, 161
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 14, 399, 592
BstDEI CTNAG 3 cut(s) 330, 759, 806
BstFNI CGCG 1 cut(s) 260
BstKTI GATC 2 cut(s) 76, 82
BstMAI GTCTC 2 cut(s) 357, 747
BstMBI GATC 2 cut(s) 73, 79
BstMCI CGRYCG 1 cut(s) 248
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 2 cut(s) 221, 675
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 4 cut(s) 219, 378, 659, 673
BstSFI CTRYAG 1 cut(s) 203
BstSLI GKGCMC 1 cut(s) 612
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 236, 239
BstV2I GAAGAC 1 cut(s) 107
BstXI CCANNNNNNTGG 2 cut(s) 98, 652
BsuRI GGCC 3 cut(s) 301, 610, 725
BtsIMutI CAGTG 2 cut(s) 43, 287
Cac8I GCNNGC 3 cut(s) 14, 399, 592
CaiI CAGNNNCTG 1 cut(s) 152
CciI TCATGA 2 cut(s) 76, 82
Cfr13I GGNCC 7 cut(s) 48, 299, 315, 608, 609, 723, 747
CviAII CATG 6 cut(s) 77, 83, 92, 341, 712, 772
DdeI CTNAG 3 cut(s) 330, 759, 806
DpnI GATC 2 cut(s) 75, 81
DpnII GATC 2 cut(s) 73, 79
Eam1104I CTCTTC 3 cut(s) 31, 156, 161
EarI CTCTTC 3 cut(s) 31, 156, 161
Ecl136II GAGCTC 2 cut(s) 709, 797
Eco24I GRGCYC 5 cut(s) 285, 612, 645, 711, 799
Eco31I GGTCTC 1 cut(s) 357
Eco47I GGWCC 3 cut(s) 48, 315, 747
Eco53kI GAGCTC 2 cut(s) 709, 797
EcoICRI GAGCTC 2 cut(s) 709, 797
EcoO109I RGGNCCY 1 cut(s) 315
EcoRII CCWGG 2 cut(s) 219, 673
EcoT38I GRGCYC 5 cut(s) 285, 612, 645, 711, 799
FaeI CATG 6 cut(s) 80, 86, 95, 344, 715, 775
FaiI YATR 9 cut(s) 78, 84, 93, 311, 342, 713, 717, 719, 773
FaqI GGGAC 1 cut(s) 328
FatI CATG 6 cut(s) 76, 82, 91, 340, 711, 771
FbaI TGATCA 2 cut(s) 73, 79
Fnu4HI GCNGC 2 cut(s) 225, 228
FriOI GRGCYC 5 cut(s) 285, 612, 645, 711, 799
Fsp4HI GCNGC 2 cut(s) 225, 228
FspBI CTAG 1 cut(s) 591
GluI GCNGC 2 cut(s) 225, 228
GsaI CCCAGC 1 cut(s) 696
GsuI CTGGAG 3 cut(s) 203, 341, 560
HaeIII GGCC 3 cut(s) 301, 610, 725
HapII CCGG 3 cut(s) 244, 380, 660
Hin1II CATG 6 cut(s) 80, 86, 95, 344, 715, 775
HinfI GANTC 4 cut(s) 20, 187, 500, 755
HpaII CCGG 3 cut(s) 244, 380, 660
HphI GGTGA 3 cut(s) 433, 698, 716
Hpy188I TCNGA 2 cut(s) 154, 760
Hpy188III TCNNGA 6 cut(s) 77, 83, 358, 388, 750, 800
HpyAV CCTTC 5 cut(s) 204, 418, 616, 625, 677
HpyCH4V TGCA 3 cut(s) 16, 346, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 3 cut(s) 330, 759, 806
Hsp92II CATG 6 cut(s) 80, 86, 95, 344, 715, 775
KflI GGGWCCC 1 cut(s) 315
Ksp22I TGATCA 2 cut(s) 73, 79
Kzo9I GATC 2 cut(s) 73, 79
LmnI GCTCC 6 cut(s) 222, 246, 288, 544, 794, 802
Lsp1109I GCAGC 2 cut(s) 236, 239
MaeI CTAG 1 cut(s) 591
MaeIII GTNAC 1 cut(s) 464
MalI GATC 2 cut(s) 75, 81
MboI GATC 2 cut(s) 73, 79
MboII GAAGA 8 cut(s) 18, 80, 107, 107, 173, 178, 280, 499
MhlI GDGCHC 5 cut(s) 285, 612, 645, 711, 799
MluCI AATT 3 cut(s) 231, 370, 408
MlyI GAGTC 3 cut(s) 14, 181, 749
MmeI TCCRAC 2 cut(s) 85, 143
MseI TTAA 1 cut(s) 829
MslI CAYNNNNRTG 4 cut(s) 81, 96, 650, 716
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 3 cut(s) 244, 380, 660
MspR9I CCNGG 4 cut(s) 221, 380, 661, 675
MvaI CCWGG 2 cut(s) 221, 675
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 544
NciI CCSGG 2 cut(s) 380, 661
NdeII GATC 2 cut(s) 73, 79
NheI GCTAGC 1 cut(s) 590
NlaIII CATG 6 cut(s) 80, 86, 95, 344, 715, 775
NlaIV GGNNCC 9 cut(s) 242, 284, 300, 316, 317, 546, 610, 658, 748
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 464
NspI RCATGY 1 cut(s) 344
NspV TTCGAA 1 cut(s) 503
PagI TCATGA 2 cut(s) 76, 82
PfeI GAWTC 1 cut(s) 500
PflMI CCANNNNNTGG 2 cut(s) 413, 618
PkrI GCNGC 2 cut(s) 226, 229
PleI GAGTC 3 cut(s) 14, 181, 749
PpsI GAGTC 3 cut(s) 14, 181, 749
PpuMI RGGWCCY 1 cut(s) 315
Psp124BI GAGCTC 2 cut(s) 711, 799
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 219, 673
PspFI CCCAGC 1 cut(s) 692
PspGI CCWGG 2 cut(s) 219, 673
PspN4I GGNNCC 9 cut(s) 242, 284, 300, 316, 317, 546, 610, 658, 748
PspOMI GGGCCC 1 cut(s) 608
PspPI GGNCC 7 cut(s) 48, 299, 315, 608, 609, 723, 747
PspPPI RGGWCCY 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 527
RseI CAYNNNNRTG 4 cut(s) 81, 96, 650, 716
SacI GAGCTC 2 cut(s) 711, 799
SaqAI TTAA 1 cut(s) 829
SatI GCNGC 2 cut(s) 225, 228
Sau3AI GATC 2 cut(s) 73, 79
Sau96I GGNCC 7 cut(s) 48, 299, 315, 608, 609, 723, 747
SchI GAGTC 3 cut(s) 14, 181, 749
ScrFI CCNGG 4 cut(s) 221, 380, 661, 675
SduI GDGCHC 5 cut(s) 285, 612, 645, 711, 799
SfcI CTRYAG 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 503
SinI GGWCC 3 cut(s) 48, 315, 747
SmiMI CAYNNNNRTG 4 cut(s) 81, 96, 650, 716
Sse9I AATT 3 cut(s) 231, 370, 408
SsiI CCGC 1 cut(s) 258
SspI AATATT 1 cut(s) 131
SspMI CTAG 1 cut(s) 591
SstI GAGCTC 2 cut(s) 711, 799
StyD4I CCNGG 4 cut(s) 219, 378, 659, 673
TaqI TCGA 5 cut(s) 236, 503, 509, 515, 823
TasI AATT 3 cut(s) 231, 370, 408
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 1 cut(s) 829
Tru9I TTAA 1 cut(s) 829
TscAI CASTG 2 cut(s) 50, 294
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 2 cut(s) 224, 227
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 4 cut(s) 81, 108, 696, 791
TspRI CASTG 2 cut(s) 50, 294
Van91I CCANNNNNTGG 2 cut(s) 413, 618
VpaK11BI GGWCC 3 cut(s) 48, 315, 747
XapI RAATTY 1 cut(s) 370
XceI RCATGY 1 cut(s) 344
XspI CTAG 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.