Rroxscaffold_3G00269710

mizu-kussei

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015885
Physical Location & Seq
Reverse (-)
62495241 .. 62496077
837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269710.1

Sequence Viewer

Length: 837 bp
ATGGCAAAGAGCTTGCAGGACTCCTCTTCCAAAAGACACTTCCACTGGACCAACAAAGTTGGCAATGAAGATGATCATGAACATGATAACCATGAAGATGGAGTTGGAGTAGGAGTAGCTCTTCCAACCTTGAAATCTTCTCTGTCAAAGGCAGTTTCTGATGAGGAAGAGAAGAGAAAGGAGGGTCAAGCGACTCAAAGGGTTCAGCTACAGAGAAGGAAGCTCCAGGCAGCAGCAATTTCGAGGCTCCGGTCGGTGCTGACCGCGTTTGGGAAGAACAGGTCAGGGCTCCCACTGGGTCTGGGGCCGAGAGTTATAGGGACCCTTTTTGGGTCTAAGAAAGGGCATGTGCATTTTGCTCTCCAGAGAGACCCAAATTCACACCCGGCTTTCCTGATTGAGCTTGCTACTCCAATTAGTGGACTGGTGAAGGAAATGGCTTCTGGGCTTGTGAGGATTGCTTTGGAGTGTGACAAGGAGAAGGAGGAGGAGAAGAAAACAGAGGATTCGAAATCCAAGTCGAAAAGTTCAGCTGTGAGGTTGCTGGAGGAGCCTGTGTGGAGGACTTACTGCAATGGGAAGAAGTCTGGGTTTGCTAGCAGGAGGGAATGTGGGCCCAAAGAGTGGAAGGTGTTGAAGGCTGTTGAGCCCATTTCAATGGGTGCTGGGGTCTTGCCTCCTGGGAATGAAGGTGAAGCTGGGTGTGATGGTGAGCTCATGTATATGAGGGCCAAGTTCGAGAGGGTTGTTGGGTCCAGAGACTCTGAGGCTTTCTACATGATGAACCCAGATAGCAATGGAGCTCCTGAACTCAGTATCTACTTGCTTCGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

278

Amino Acids

30.63

Weight (kDa)

9.04

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 105 - 277 1.8e-68 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23100
fragaria_vesca FvH4_5g12900
malus_domestica MD06G1209400.v1.1 MD14G1219900.v1.1
prunus_persica Prupe.5G214500_v2.0.a1
pyrus_communis pycom06g18700
rosa_chinensis RchiOBHm_Chr7g0184341
rosa_laevigata RLG00000004985
rosa_multiflora Rmu_sc0001557.1_g000024
rosa_roxburghii Rroxscaffold_3G00269710
rosa_samantha Rh7AG080900 Rh7BG066300 Rh7CG066600 Rh7DG066300
rosa_wichuraiana Rw7G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 419, 624
AccII CGCG 1 cut(s) 266
AciI CCGC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 376
AfiI CCNNNNNNNGG 4 cut(s) 270, 330, 419, 624
AgsI TTSAA 3 cut(s) 133, 637, 657
AjnI CCWGG 2 cut(s) 225, 679
AjuI GAANNNNNNNTTGG 4 cut(s) 23, 55, 87, 119
AluBI AGCT 9 cut(s) 12, 119, 208, 223, 403, 533, 698, 715, 803
AluI AGCT 9 cut(s) 12, 119, 208, 223, 403, 533, 698, 715, 803
Alw21I GWGCWC 2 cut(s) 717, 805
Alw26I GTCTC 2 cut(s) 363, 753
AlwNI CAGNNNCTG 1 cut(s) 158
AoxI GGCC 3 cut(s) 305, 614, 729
ApaI GGGCCC 1 cut(s) 618
ApeKI GCWGC 2 cut(s) 230, 233
ApoI RAATTY 1 cut(s) 376
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AspS9I GGNCC 7 cut(s) 48, 305, 321, 614, 615, 729, 753
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 3 cut(s) 439, 704, 722
AsuII TTCGAA 1 cut(s) 509
AsuNHI GCTAGC 1 cut(s) 596
AvaII GGWCC 3 cut(s) 48, 321, 753
BaeGI GKGCMC 1 cut(s) 618
BanII GRGCYC 5 cut(s) 291, 618, 651, 717, 805
Bbv12I GWGCWC 2 cut(s) 717, 805
BbvI GCAGC 2 cut(s) 242, 245
BccI CCATC 2 cut(s) 92, 701
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BciT130I CCWGG 2 cut(s) 227, 681
BclI TGATCA 1 cut(s) 73
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 2 cut(s) 363, 753
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 209
BisI GCNGC 2 cut(s) 231, 234
BlsI GCNGC 2 cut(s) 232, 235
Bme1390I CCNGG 3 cut(s) 227, 386, 681
Bme18I GGWCC 3 cut(s) 48, 321, 753
BmgT120I GGNCC 7 cut(s) 48, 305, 321, 614, 615, 729, 753
BmiI GGNNCC 8 cut(s) 248, 290, 306, 322, 323, 552, 616, 754
BmrFI CCNGG 3 cut(s) 227, 386, 681
BmrI ACTGGG 1 cut(s) 305
BmtI GCTAGC 1 cut(s) 600
BmuI ACTGGG 1 cut(s) 305
BpmI CTGGAG 3 cut(s) 209, 347, 566
Bpu14I TTCGAA 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 680
BsaWI WCCGGW 1 cut(s) 249
BsaXI ACNNNNNCTCC 8 cut(s) 93, 99, 123, 129, 542, 572, 595, 625
Bsc4I CCNNNNNNNGG 4 cut(s) 270, 330, 419, 624
Bse1I ACTGG 3 cut(s) 50, 300, 429
Bse3DI GCAATG 3 cut(s) 70, 580, 802
BseBI CCWGG 2 cut(s) 227, 681
BseDI CCNNGG 1 cut(s) 680
BseLI CCNNNNNNNGG 4 cut(s) 270, 330, 419, 624
BseMI GCAATG 3 cut(s) 70, 580, 802
BseMII CTCAG 2 cut(s) 756, 826
BseNI ACTGG 3 cut(s) 50, 300, 429
BseRI GAGGAG 4 cut(s) 13, 500, 503, 563
BseSI GKGCMC 1 cut(s) 618
BseXI GCAGC 2 cut(s) 242, 245
BseYI CCCAGC 2 cut(s) 665, 698
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 3 cut(s) 307, 616, 731
BsiEI CGRYCG 1 cut(s) 254
BsiHKAI GWGCWC 2 cut(s) 717, 805
BsiSI CCGG 2 cut(s) 250, 386
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 4 cut(s) 270, 330, 419, 624
BsmAI GTCTC 2 cut(s) 363, 753
BsmFI GGGAC 1 cut(s) 334
BsnI GGCC 3 cut(s) 307, 616, 731
Bso31I GGTCTC 1 cut(s) 363
Bsp119I TTCGAA 1 cut(s) 509
Bsp120I GGGCCC 1 cut(s) 614
Bsp1286I GDGCHC 5 cut(s) 291, 618, 651, 717, 805
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 1 cut(s) 264
BspANI GGCC 3 cut(s) 307, 616, 731
BspCNI CTCAG 2 cut(s) 757, 825
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 8 cut(s) 248, 290, 306, 322, 323, 552, 616, 754
BspOI GCTAGC 1 cut(s) 600
BspQI GCTCTTC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 363
BsrDI GCAATG 3 cut(s) 70, 580, 802
BsrI ACTGG 3 cut(s) 50, 300, 429
BssECI CCNNGG 1 cut(s) 680
BssMI GATC 1 cut(s) 73
Bst2UI CCWGG 2 cut(s) 227, 681
Bst6I CTCTTC 4 cut(s) 31, 126, 162, 167
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 14, 405, 598
BstDEI CTNAG 3 cut(s) 336, 765, 812
BstFNI CGCG 1 cut(s) 266
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 2 cut(s) 363, 753
BstMBI GATC 1 cut(s) 73
BstMCI CGRYCG 1 cut(s) 254
BstMWI GCNNNNNNNGC 1 cut(s) 550
BstNI CCWGG 2 cut(s) 227, 681
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 3 cut(s) 225, 384, 679
BstSFI CTRYAG 1 cut(s) 209
BstSLI GKGCMC 1 cut(s) 618
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 2 cut(s) 242, 245
BstXI CCANNNNNNTGG 2 cut(s) 98, 658
BsuRI GGCC 3 cut(s) 307, 616, 731
BtsIMutI CAGTG 2 cut(s) 43, 293
Cac8I GCNNGC 3 cut(s) 14, 405, 598
CaiI CAGNNNCTG 1 cut(s) 158
CciI TCATGA 1 cut(s) 76
Cfr13I GGNCC 7 cut(s) 48, 305, 321, 614, 615, 729, 753
CviAII CATG 6 cut(s) 77, 83, 92, 347, 718, 778
DdeI CTNAG 3 cut(s) 336, 765, 812
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
Eam1104I CTCTTC 4 cut(s) 31, 126, 162, 167
EarI CTCTTC 4 cut(s) 31, 126, 162, 167
Ecl136II GAGCTC 2 cut(s) 715, 803
Eco24I GRGCYC 5 cut(s) 291, 618, 651, 717, 805
Eco31I GGTCTC 1 cut(s) 363
Eco47I GGWCC 3 cut(s) 48, 321, 753
Eco53kI GAGCTC 2 cut(s) 715, 803
EcoICRI GAGCTC 2 cut(s) 715, 803
EcoO109I RGGNCCY 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 225, 679
EcoT38I GRGCYC 5 cut(s) 291, 618, 651, 717, 805
FaeI CATG 6 cut(s) 80, 86, 95, 350, 721, 781
FaiI YATR 9 cut(s) 78, 84, 93, 317, 348, 719, 723, 725, 779
FaqI GGGAC 1 cut(s) 334
FatI CATG 6 cut(s) 76, 82, 91, 346, 717, 777
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 231, 234
FriOI GRGCYC 5 cut(s) 291, 618, 651, 717, 805
Fsp4HI GCNGC 2 cut(s) 231, 234
FspBI CTAG 1 cut(s) 597
GluI GCNGC 2 cut(s) 231, 234
GsaI CCCAGC 2 cut(s) 669, 702
GsuI CTGGAG 3 cut(s) 209, 347, 566
HaeIII GGCC 3 cut(s) 307, 616, 731
HapII CCGG 2 cut(s) 250, 386
Hin1II CATG 6 cut(s) 80, 86, 95, 350, 721, 781
HinfI GANTC 4 cut(s) 20, 193, 506, 761
HpaII CCGG 2 cut(s) 250, 386
HphI GGTGA 3 cut(s) 439, 704, 722
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 2 cut(s) 160, 766
Hpy188III TCNNGA 6 cut(s) 77, 364, 394, 739, 756, 806
Hpy8I GTNNAC 1 cut(s) 422
HpyAV CCTTC 6 cut(s) 210, 424, 475, 622, 631, 683
HpyCH4V TGCA 3 cut(s) 16, 352, 573
HpyF10VI GCNNNNNNNGC 1 cut(s) 550
HpyF3I CTNAG 3 cut(s) 336, 765, 812
Hsp92II CATG 6 cut(s) 80, 86, 95, 350, 721, 781
KflI GGGWCCC 1 cut(s) 321
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 1 cut(s) 73
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 6 cut(s) 228, 252, 294, 550, 800, 808
Lsp1109I GCAGC 2 cut(s) 242, 245
MaeI CTAG 1 cut(s) 597
MaeIII GTNAC 1 cut(s) 470
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MhlI GDGCHC 5 cut(s) 291, 618, 651, 717, 805
MluCI AATT 3 cut(s) 237, 376, 414
MlyI GAGTC 3 cut(s) 14, 187, 755
MmeI TCCRAC 2 cut(s) 85, 149
MseI TTAA 1 cut(s) 835
MslI CAYNNNNRTG 4 cut(s) 81, 96, 656, 722
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 2 cut(s) 250, 386
MspR9I CCNGG 3 cut(s) 227, 386, 681
MvaI CCWGG 2 cut(s) 227, 681
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 1 cut(s) 550
NciI CCSGG 1 cut(s) 386
NdeII GATC 1 cut(s) 73
NheI GCTAGC 1 cut(s) 596
NlaIII CATG 6 cut(s) 80, 86, 95, 350, 721, 781
NlaIV GGNNCC 8 cut(s) 248, 290, 306, 322, 323, 552, 616, 754
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 470
NspI RCATGY 1 cut(s) 350
NspV TTCGAA 1 cut(s) 509
PagI TCATGA 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 506
PflMI CCANNNNNTGG 2 cut(s) 419, 624
PkrI GCNGC 2 cut(s) 232, 235
PleI GAGTC 3 cut(s) 14, 187, 755
PpsI GAGTC 3 cut(s) 14, 187, 755
PpuMI RGGWCCY 1 cut(s) 321
Psp124BI GAGCTC 2 cut(s) 717, 805
Psp5II RGGWCCY 1 cut(s) 321
Psp6I CCWGG 2 cut(s) 225, 679
PspFI CCCAGC 2 cut(s) 665, 698
PspGI CCWGG 2 cut(s) 225, 679
PspN4I GGNNCC 8 cut(s) 248, 290, 306, 322, 323, 552, 616, 754
PspOMI GGGCCC 1 cut(s) 614
PspPI GGNCC 7 cut(s) 48, 305, 321, 614, 615, 729, 753
PspPPI RGGWCCY 1 cut(s) 321
PstNI CAGNNNCTG 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 533
RseI CAYNNNNRTG 4 cut(s) 81, 96, 656, 722
SacI GAGCTC 2 cut(s) 717, 805
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 835
SatI GCNGC 2 cut(s) 231, 234
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 7 cut(s) 48, 305, 321, 614, 615, 729, 753
SchI GAGTC 3 cut(s) 14, 187, 755
ScrFI CCNGG 3 cut(s) 227, 386, 681
SduI GDGCHC 5 cut(s) 291, 618, 651, 717, 805
SfcI CTRYAG 1 cut(s) 209
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 3 cut(s) 48, 321, 753
SmiMI CAYNNNNRTG 4 cut(s) 81, 96, 656, 722
Sse9I AATT 3 cut(s) 237, 376, 414
SsiI CCGC 1 cut(s) 264
SspMI CTAG 1 cut(s) 597
SstI GAGCTC 2 cut(s) 717, 805
StyD4I CCNGG 3 cut(s) 225, 384, 679
TaqI TCGA 5 cut(s) 242, 509, 521, 738, 829
TasI AATT 3 cut(s) 237, 376, 414
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 1 cut(s) 835
Tru9I TTAA 1 cut(s) 835
TscAI CASTG 2 cut(s) 50, 300
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 230, 233
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 5 cut(s) 81, 93, 108, 702, 797
TspRI CASTG 2 cut(s) 50, 300
Van91I CCANNNNNTGG 2 cut(s) 419, 624
VpaK11BI GGWCC 3 cut(s) 48, 321, 753
XapI RAATTY 1 cut(s) 376
XceI RCATGY 1 cut(s) 350
XspI CTAG 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.