MD06G1209400.v1.1

mizu-kussei

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015885
Physical Location & Seq
Forward (+)
34277897 .. 34279243
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1209400.v1.1.491

Sequence Viewer

Length: 792 bp
ATGAGCATGGCAAGGAACTTGCAGGACTCCTCCTCCAAGAGACACTTCCACTGGACAAACAAAGTTGGCAACGAAGAAGAAGAAGAAGATGTTGATCATGTAGTTCTTCCGAGTCTCAACTCCTCTTCCTCCTCCTCCTCCTCGTCGAAAACAAGATCCGATGAGGAGGAGAAGAAACAAGAGCATGATCATCATCAAAAACAACAGCATGGTCATGTGGCTCATCAACTTCCGAGGAGGAAACTGCAGGCGGCTGCGGTTTCCAGGATGAGGTCAGTTCTTAACGCTTTTGGTAAGAACCGATCCAGCCTACCACTCGGCCTTGGACCTCGAGTGGTAGGCACACTTTTCGGCTCCAGGCGTGGCCACGTACACTTTGCACTCCAGAGAGACCCAAGCTCACACCCGGCCTTCTTGATCGAGCTCGCCACCCCGATCAGCGGGTTGGTTAAAGAAATGGCATCCGGGCTCGTCAGAATTGCGTTGGAGTGTGACAAGGATCACAAAGACGATCATCAGAAAAAGAAAGACGAGAAAGCTCCGAGGTTGCTGGAAGAGCCAGTGTGGAGAACTTTTTGCAACGGTAAGAAATATGGTTTTGCTAGCAGAAGAGAATGTGGGGCCAAGGAGTGGAAGGTGTTGAAAGCTGTGGAGCCCATTTCAATGGGGGCTGGTGTGCTGCCGCCGTCTGCGGCGGTGGAAGGGAATGAAGCTGAAGCAGGGTCCGAATCCGACGGGGAACTAATGTACATGAGGGCTCCTGAGCTCAGTGTCTACTTGCTCCGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

264

Amino Acids

29.27

Weight (kDa)

8.68

Isoelectric Point (pI)

66.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 112 - 253 5.3e-48 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23100
fragaria_vesca FvH4_5g12900
malus_domestica MD06G1209400.v1.1 MD14G1219900.v1.1
prunus_persica Prupe.5G214500_v2.0.a1
pyrus_communis pycom06g18700
rosa_chinensis RchiOBHm_Chr7g0184341
rosa_laevigata RLG00000004985
rosa_multiflora Rmu_sc0001557.1_g000024
rosa_roxburghii Rroxscaffold_3G00269710
rosa_samantha Rh7AG080900 Rh7BG066300 Rh7CG066600 Rh7DG066300
rosa_wichuraiana Rw7G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 630
AccI GTMKAC 1 cut(s) 774
AciI CCGC 6 cut(s) 251, 257, 441, 683, 692, 695
AclWI GGATC 3 cut(s) 150, 297, 507
AcoI YGGCCR 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 735
AfaI GTAC 2 cut(s) 372, 749
AfiI CCNNNNNNNGG 3 cut(s) 270, 440, 630
AgsI TTSAA 2 cut(s) 643, 663
AjnI CCWGG 2 cut(s) 263, 356
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 6 cut(s) 399, 424, 539, 647, 713, 766
AluI AGCT 6 cut(s) 399, 424, 539, 647, 713, 766
Alw21I GWGCWC 2 cut(s) 426, 768
Alw26I GTCTC 3 cut(s) 34, 119, 384
AlwI GGATC 3 cut(s) 150, 297, 507
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 4 cut(s) 319, 364, 408, 621
ApeKI GCWGC 2 cut(s) 254, 679
AspS9I GGNCC 3 cut(s) 326, 621, 723
AsuC2I CCSGG 2 cut(s) 407, 466
AsuNHI GCTAGC 1 cut(s) 602
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 326, 723
BalI TGGCCA 1 cut(s) 366
BanII GRGCYC 5 cut(s) 426, 471, 657, 760, 768
Bbv12I GWGCWC 2 cut(s) 426, 768
BbvI GCAGC 2 cut(s) 241, 666
BceAI ACGGC 1 cut(s) 670
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BciT130I CCWGG 2 cut(s) 265, 358
BclI TGATCA 2 cut(s) 94, 187
BcnI CCSGG 2 cut(s) 407, 466
BcoDI GTCTC 3 cut(s) 34, 119, 384
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 245
BisI GCNGC 5 cut(s) 252, 255, 680, 683, 693
BlsI GCNGC 5 cut(s) 253, 256, 681, 684, 694
Bme1390I CCNGG 4 cut(s) 265, 358, 407, 466
Bme18I GGWCC 2 cut(s) 326, 723
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 3 cut(s) 326, 621, 723
BmiI GGNNCC 5 cut(s) 355, 622, 654, 724, 759
BmrFI CCNGG 4 cut(s) 265, 358, 407, 466
BmsI GCATC 1 cut(s) 470
BmtI GCTAGC 1 cut(s) 606
BpmI CTGGAG 2 cut(s) 340, 368
Bpu10I CCTNAGC 1 cut(s) 762
BpuMI CCSGG 2 cut(s) 407, 466
BsaAI YACGTR 1 cut(s) 370
BsaBI GATNNNNATC 2 cut(s) 93, 192
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 4 cut(s) 233, 322, 542, 624
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 440, 630
Bse1I ACTGG 2 cut(s) 56, 560
Bse8I GATNNNNATC 2 cut(s) 93, 192
BseBI CCWGG 2 cut(s) 265, 358
BseDI CCNNGG 4 cut(s) 233, 322, 542, 624
BseGI GGATG 2 cut(s) 273, 461
BseJI GATNNNNATC 2 cut(s) 93, 192
BseLI CCNNNNNNNGG 3 cut(s) 270, 440, 630
BseMII CTCAG 2 cut(s) 753, 781
BseNI ACTGG 2 cut(s) 56, 560
BseXI GCAGC 2 cut(s) 241, 666
BshFI GGCC 4 cut(s) 321, 366, 410, 623
BsiHKAI GWGCWC 2 cut(s) 426, 768
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 2 cut(s) 407, 465
BslI CCNNNNNNNGG 3 cut(s) 270, 440, 630
BsmAI GTCTC 3 cut(s) 34, 119, 384
BsnI GGCC 4 cut(s) 321, 366, 410, 623
Bso31I GGTCTC 1 cut(s) 384
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 5 cut(s) 426, 471, 657, 760, 768
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
BspACI CCGC 6 cut(s) 251, 257, 441, 683, 692, 695
BspANI GGCC 4 cut(s) 321, 366, 410, 623
BspCNI CTCAG 2 cut(s) 754, 780
BspLI GGNNCC 5 cut(s) 355, 622, 654, 724, 759
BspMAI CTGCAG 1 cut(s) 249
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 3 cut(s) 150, 297, 507
BspQI GCTCTTC 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 384
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 2 cut(s) 56, 560
BssECI CCNNGG 4 cut(s) 233, 322, 542, 624
BssMI GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
BssT1I CCWWGG 2 cut(s) 322, 624
Bst2UI CCWGG 2 cut(s) 265, 358
Bst4CI ACNGT 1 cut(s) 584
Bst6I CTCTTC 3 cut(s) 130, 549, 604
BstAUI TGTACA 1 cut(s) 747
BstBAI YACGTR 1 cut(s) 370
BstC8I GCNNGC 3 cut(s) 249, 426, 604
BstDEI CTNAG 2 cut(s) 762, 767
BstF5I GGATG 2 cut(s) 273, 461
BstKTI GATC 8 cut(s) 97, 158, 190, 305, 420, 438, 502, 514
BstMAI GTCTC 3 cut(s) 34, 119, 384
BstMBI GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNI CCWGG 2 cut(s) 265, 358
BstSCI CCNGG 4 cut(s) 263, 356, 405, 464
BstSFI CTRYAG 1 cut(s) 245
BstV1I GCAGC 2 cut(s) 241, 666
BstX2I RGATCY 1 cut(s) 155
BstXI CCANNNNNNTGG 1 cut(s) 664
BstYI RGATCY 1 cut(s) 155
BsuRI GGCC 4 cut(s) 321, 366, 410, 623
BtsCI GGATG 2 cut(s) 273, 461
BtsIMutI CAGTG 3 cut(s) 49, 567, 775
Cac8I GCNNGC 3 cut(s) 249, 426, 604
Cfr13I GGNCC 3 cut(s) 326, 621, 723
Csp6I GTAC 2 cut(s) 371, 748
CviAII CATG 6 cut(s) 7, 98, 185, 209, 215, 751
CviQI GTAC 2 cut(s) 371, 748
DdeI CTNAG 2 cut(s) 762, 767
DpnI GATC 8 cut(s) 96, 157, 189, 304, 419, 437, 501, 513
DpnII GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 3 cut(s) 130, 549, 604
EarI CTCTTC 3 cut(s) 130, 549, 604
Ecl136II GAGCTC 2 cut(s) 424, 766
Eco130I CCWWGG 2 cut(s) 322, 624
Eco24I GRGCYC 5 cut(s) 426, 471, 657, 760, 768
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 2 cut(s) 326, 723
Eco53kI GAGCTC 2 cut(s) 424, 766
Eco57I CTGAAG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 2 cut(s) 424, 766
EcoRII CCWGG 2 cut(s) 263, 356
EcoT14I CCWWGG 2 cut(s) 322, 624
EcoT38I GRGCYC 5 cut(s) 426, 471, 657, 760, 768
ErhI CCWWGG 2 cut(s) 322, 624
FaeI CATG 6 cut(s) 10, 101, 188, 212, 218, 754
FaiI YATR 7 cut(s) 8, 99, 186, 210, 216, 594, 752
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FatI CATG 6 cut(s) 6, 97, 184, 208, 214, 750
FauI CCCGC 1 cut(s) 434
FbaI TGATCA 2 cut(s) 94, 187
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 5 cut(s) 252, 255, 680, 683, 693
FokI GGATG 2 cut(s) 280, 448
FriOI GRGCYC 5 cut(s) 426, 471, 657, 760, 768
Fsp4HI GCNGC 5 cut(s) 252, 255, 680, 683, 693
FspBI CTAG 1 cut(s) 603
GluI GCNGC 5 cut(s) 252, 255, 680, 683, 693
GsuI CTGGAG 2 cut(s) 340, 368
HaeIII GGCC 4 cut(s) 321, 366, 410, 623
HapII CCGG 2 cut(s) 407, 465
Hin1II CATG 6 cut(s) 10, 101, 188, 212, 218, 754
HinfI GANTC 4 cut(s) 26, 112, 728, 786
HpaII CCGG 2 cut(s) 407, 465
Hpy166II GTNNAC 2 cut(s) 373, 775
Hpy188I TCNGA 9 cut(s) 111, 160, 234, 476, 519, 543, 727, 733, 785
Hpy188III TCNNGA 3 cut(s) 385, 415, 761
Hpy8I GTNNAC 2 cut(s) 373, 775
Hpy99I CGWCG 2 cut(s) 148, 737
HpyAV CCTTC 3 cut(s) 421, 628, 695
HpyCH4III ACNGT 1 cut(s) 584
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 22, 247, 380, 579
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 2 cut(s) 762, 767
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 6 cut(s) 10, 101, 188, 212, 218, 754
Ksp22I TGATCA 2 cut(s) 94, 187
Kzo9I GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 5 cut(s) 359, 544, 652, 763, 786
Lsp1109I GCAGC 2 cut(s) 241, 666
LweI GCATC 1 cut(s) 470
MaeI CTAG 1 cut(s) 603
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 491
MalI GATC 8 cut(s) 96, 157, 189, 304, 419, 437, 501, 513
MboI GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 5 cut(s) 426, 471, 657, 760, 768
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 1 cut(s) 477
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 2 cut(s) 20, 121
MmeI TCCRAC 2 cut(s) 465, 756
Mox20I TGGCCA 1 cut(s) 366
MscI TGGCCA 1 cut(s) 366
MseI TTAA 2 cut(s) 282, 450
MslI CAYNNNNRTG 2 cut(s) 213, 662
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 441
MspI CCGG 2 cut(s) 407, 465
MspR9I CCNGG 4 cut(s) 265, 358, 407, 466
MvaI CCWGG 2 cut(s) 265, 358
MwoI GCNNNNNNNGC 1 cut(s) 556
NciI CCSGG 2 cut(s) 407, 466
NdeII GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 6 cut(s) 10, 101, 188, 212, 218, 754
NlaIV GGNNCC 5 cut(s) 355, 622, 654, 724, 759
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 549
PfeI GAWTC 1 cut(s) 728
PflMI CCANNNNNTGG 1 cut(s) 630
PfoI TCCNGGA 1 cut(s) 263
PkrI GCNGC 5 cut(s) 253, 256, 681, 684, 694
PleI GAGTC 2 cut(s) 20, 120
PpsI GAGTC 2 cut(s) 20, 120
Ppu21I YACGTR 1 cut(s) 370
Psp124BI GAGCTC 2 cut(s) 426, 768
Psp6I CCWGG 2 cut(s) 263, 356
PspGI CCWGG 2 cut(s) 263, 356
PspN4I GGNNCC 5 cut(s) 355, 622, 654, 724, 759
PspPI GGNCC 3 cut(s) 326, 621, 723
PspXI VCTCGAGB 1 cut(s) 330
PstI CTGCAG 1 cut(s) 249
PsuI RGATCY 1 cut(s) 155
RsaI GTAC 2 cut(s) 372, 749
RsaNI GTAC 2 cut(s) 371, 748
RseI CAYNNNNRTG 2 cut(s) 213, 662
SacI GAGCTC 2 cut(s) 426, 768
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 2 cut(s) 282, 450
SatI GCNGC 5 cut(s) 252, 255, 680, 683, 693
Sau3AI GATC 8 cut(s) 94, 155, 187, 302, 417, 435, 499, 511
Sau96I GGNCC 3 cut(s) 326, 621, 723
SchI GAGTC 2 cut(s) 20, 121
ScrFI CCNGG 4 cut(s) 265, 358, 407, 466
SduI GDGCHC 5 cut(s) 426, 471, 657, 760, 768
SfaNI GCATC 1 cut(s) 470
SfcI CTRYAG 1 cut(s) 245
Sfr274I CTCGAG 1 cut(s) 330
SinI GGWCC 2 cut(s) 326, 723
SlaI CTCGAG 1 cut(s) 330
SmiMI CAYNNNNRTG 2 cut(s) 213, 662
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse9I AATT 1 cut(s) 477
SsiI CCGC 6 cut(s) 251, 257, 441, 683, 692, 695
SspMI CTAG 1 cut(s) 603
SstI GAGCTC 2 cut(s) 426, 768
StyD4I CCNGG 4 cut(s) 263, 356, 405, 464
StyI CCWWGG 2 cut(s) 322, 624
TaaI ACNGT 1 cut(s) 584
TaiI ACGT 1 cut(s) 372
TaqI TCGA 3 cut(s) 146, 331, 420
TasI AATT 1 cut(s) 477
TatI WGTACW 1 cut(s) 747
TauI GCSGC 3 cut(s) 254, 685, 695
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 2 cut(s) 282, 450
Tru9I TTAA 2 cut(s) 282, 450
TscAI CASTG 3 cut(s) 56, 567, 775
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 2 cut(s) 254, 679
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 1 cut(s) 723
TspRI CASTG 3 cut(s) 56, 567, 775
Van91I CCANNNNNTGG 1 cut(s) 630
VpaK11BI GGWCC 2 cut(s) 326, 723
XhoI CTCGAG 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.