FvH4_5g14390

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011359
Physical Location & Seq
Forward (+)
8137772 .. 8140377
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14390.t1

Sequence Viewer

Length: 1416 bp
ATGGTTTCTGAGAGGTACCAATGCAAAAGTGTATGTGTAATTGGGGCTGGACCGTCGGGCCTTGTTGCTGCAAGAGAGCTGAGGAAAGAAGGTCACAGAGTGGTGGTGTTGGAACAAAACCATGATGTAGGAGGTCAATGGCTGTATGACCCAAATGTGGAGGGAGAGGACCCTTTAGGAAGGAACACTACTAGTCCTACTACTAGTACTAATTCTCAACATCTCAAAGTCCACAGTAGTCTTTATACCTCTTTAAGGCTTACATCCCCTAGAGAGATCATGGGGTTCACTGATTTTCCATTTTCAGTTAAGAAGGGTAGAGACATGAGAAGGTTTCCTGGGCATAGGGAACTTCTTTTGTACCTTCAAGACTTCTGTGACTGGTTTGGGTTAAGGGATTTGATTAGGTTCAATACTAGGGTGGCTTATGTGGGAATGCTGCAGGATCATGTAACGGGATGCCAAGATTTGAAATGGCTCGTTAAGAGTGTGCAGAAGAAGACCCAGATTGTGATGGAAGAGGTGTTTGATTCTGTGGTTGTTGCCACTGGCCATTACTCTCAGCCTAGATTGCCTTCCATTAAAGGAATGGATTCATGGAAGAGGAAGCAAATGCACAGTCACATTTACAGGGATTCCAAGCCTTTCCGCAATGAGGTTGTGGTTGTGGTTGGAACTTCACTAAGTGGGCAAGACATATCAATGGAGCTAGTAGAAGTGGCAAAGGCAGTGTACCTCAGCGCAAGATCCATGAACAAAATCTCTGAGGGCTTATCAAAGGTCATTTCCAAGCATGATACTTTGGATCTTCGTCCACAGATAGAGTCCCTTCAAGAAGATGGAAAGGTATTGTTTGTGGATGGTTCTTGGGTAATTGCAGACACTATCATCTACTGCACAGGGTATTCATACACATTCCCATTCCTTGACACCAAAGGAATAGTAGCTATTGATGATGACAGGGTGGGTCCTTTGTATGAGCACACCTTCCCTCCCTCACTTGCTCCTTCTTTATCTTTTATTGGCATTCCTAGAAAGATCATAGGGTTCCCTTTCTTTGAGTCACAAGCAAAATGGATAGCTCAACTGCTCTCCGGAAAAAGAACATTGCCATCATGGGAGGAAATGATGCAGTCCATTAAGGACTTCTACCACTCAAGGGATGTTGCTGGCATCCCAAAGTATCAGACTCATGATATTGCAGAGTTTGAGTATTGTGACAAATATGGAGATCATGTTGGGTTCCCACATTTAGAGGAATGGAGGAAAGAGCTTTGTCTTTCTGCCTTGAGAAATTCCGAGACCAACTTAGAAACATATAGAGATTCGCTGGATGATCATGACTTGCTTCAAGAGGCTCTTCAGAGTCCACATTTCACTCAACAAGAACAAGGAGCTCAAGATTTTCCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like K_oxygenase

Protein Analysis

472

Amino Acids

53.74

Weight (kDa)

6.03

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 6 - 47 6.1e-06 Thi4 family
FMO-like PF00743 9 - 252 5.4e-29 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 10 - 309 5.6e-17 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 10 - 206 2.5e-07 HI0933-like protein Rossmann domain
FAD_binding_3 PF01494 10 - 41 4.2e-06 FAD binding domain
DAO PF01266 11 - 45 3.2e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 13 - 47 1e-10 NAD(P)-binding Rossmann-like domain
Pyr_redox_3 PF13738 177 - 326 4.8e-15 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 268 - 398 3.5e-16 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07800
fragaria_vesca FvH4_5g14390
malus_domestica MD06G1199100.v1.1 MD14G1206500.v1.1 MD14G1206800.v1.1 MD14G1207200.v1.1
prunus_persica Prupe.5G199900_v2.0.a1
pyrus_communis pycom14g17100 pycom14g17110 pycom14g17120
rosa_chinensis RchiOBHm_Chr7g0182301
rosa_laevigata RLG00000005134
rosa_multiflora Rmu_sc0000003.1_g000007
rosa_roxburghii Rroxscaffold_3G00271340
rosa_rugosa Rorug06G0446900
rosa_samantha Rh7AG049800 Rh7BG049900 Rh7CG051200 Rh7DG049100
rosa_wichuraiana Rw7G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 15
AccB1I GGYRCC 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 1094
AciI CCGC 1 cut(s) 649
AclWI GGATC 3 cut(s) 453, 741, 813
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 1 cut(s) 1294
AcuI CTGAAG 1 cut(s) 1346
AdeI CACNNNGTG 2 cut(s) 100, 686
AfaI GTAC 4 cut(s) 17, 208, 362, 734
AfiI CCNNNNNNNGG 4 cut(s) 157, 255, 655, 1159
AgsI TTSAA 5 cut(s) 368, 412, 472, 833, 1352
AhlI ACTAGT 2 cut(s) 191, 203
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 6 cut(s) 79, 709, 947, 1082, 1273, 1397
AluI AGCT 6 cut(s) 79, 709, 947, 1082, 1273, 1397
Alw21I GWGCWC 2 cut(s) 984, 1399
Alw26I GTCTC 2 cut(s) 315, 1295
AlwI GGATC 3 cut(s) 453, 741, 813
Aor13HI TCCGGA 1 cut(s) 1094
AoxI GGCC 2 cut(s) 58, 550
ApeKI GCWGC 2 cut(s) 68, 439
ApoI RAATTY 1 cut(s) 1294
ArsI GACNNNNNNTTYG 2 cut(s) 604, 636
Asp700I GAANNNNTTC 1 cut(s) 592
Asp718I GGTACC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 4 cut(s) 50, 58, 169, 968
AvaII GGWCC 3 cut(s) 50, 169, 968
BalI TGGCCA 1 cut(s) 552
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 1399
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 2 cut(s) 984, 1399
BbvCI CCTCAGC 2 cut(s) 80, 737
BbvI GCAGC 2 cut(s) 55, 426
BccI CCATC 4 cut(s) 508, 833, 854, 1120
BciT130I CCWGG 1 cut(s) 339
BclI TGATCA 1 cut(s) 1336
BcoDI GTCTC 2 cut(s) 315, 1295
BcuI ACTAGT 2 cut(s) 191, 203
BfaI CTAG 7 cut(s) 192, 204, 270, 417, 567, 710, 1032
BfmI CTRYAG 1 cut(s) 440
BisI GCNGC 2 cut(s) 69, 440
BlsI GCNGC 2 cut(s) 70, 441
BmcAI AGTACT 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 3 cut(s) 50, 169, 968
BmgT120I GGNCC 4 cut(s) 50, 58, 169, 968
BmiI GGNNCC 5 cut(s) 17, 171, 969, 1049, 1244
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 3 cut(s) 449, 1119, 1182
BpiI GAAGAC 1 cut(s) 506
Bpu10I CCTNAGC 2 cut(s) 80, 737
BpuEI CTTGAG 3 cut(s) 1141, 1309, 1383
BsaI GGTCTC 1 cut(s) 1295
BsaJI CCNNGG 1 cut(s) 338
BsaWI WCCGGW 1 cut(s) 1094
BsaXI ACNNNNNCTCC 4 cut(s) 976, 1006, 1221, 1251
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 255, 655, 1159
Bse1I ACTGG 2 cut(s) 386, 553
Bse3DI GCAATG 2 cut(s) 658, 1106
BseAI TCCGGA 1 cut(s) 1094
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 6 cut(s) 263, 464, 865, 1168, 1173, 1339
BseLI CCNNNNNNNGG 4 cut(s) 157, 255, 655, 1159
BseMI GCAATG 2 cut(s) 658, 1106
BseMII CTCAG 4 cut(s) 71, 575, 751, 756
BseNI ACTGG 2 cut(s) 386, 553
BseXI GCAGC 2 cut(s) 55, 426
BsgI GTGCAG 2 cut(s) 512, 880
BshFI GGCC 2 cut(s) 60, 552
BshNI GGYRCC 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 984, 1399
BsiSI CCGG 1 cut(s) 1095
BslFI GGGAC 1 cut(s) 811
BslI CCNNNNNNNGG 4 cut(s) 157, 255, 655, 1159
BsmAI GTCTC 2 cut(s) 315, 1295
BsmFI GGGAC 1 cut(s) 811
BsmI GAATGC 2 cut(s) 441, 1026
BsnI GGCC 2 cut(s) 60, 552
Bso31I GGTCTC 1 cut(s) 1295
Bsp1286I GDGCHC 2 cut(s) 984, 1399
Bsp13I TCCGGA 1 cut(s) 1094
Bsp143I GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
BspACI CCGC 1 cut(s) 649
BspANI GGCC 2 cut(s) 60, 552
BspCNI CTCAG 4 cut(s) 72, 574, 750, 757
BspEI TCCGGA 1 cut(s) 1094
BspHI TCATGA 2 cut(s) 1192, 1339
BspLI GGNNCC 5 cut(s) 17, 171, 969, 1049, 1244
BspMAI CTGCAG 1 cut(s) 444
BspPI GGATC 3 cut(s) 453, 741, 813
BspQI GCTCTTC 1 cut(s) 1365
BspT107I GGYRCC 1 cut(s) 15
BspTNI GGTCTC 1 cut(s) 1295
BsrDI GCAATG 2 cut(s) 658, 1106
BsrI ACTGG 2 cut(s) 386, 553
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 54, 236, 620
Bst6I CTCTTC 3 cut(s) 513, 596, 1365
BstC8I GCNNGC 1 cut(s) 1171
BstDEI CTNAG 7 cut(s) 9, 80, 561, 683, 737, 765, 1309
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 6 cut(s) 263, 464, 865, 1168, 1173, 1339
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 7 cut(s) 279, 448, 749, 808, 1041, 1234, 1339
BstMAI GTCTC 2 cut(s) 315, 1295
BstMBI GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
BstMWI GCNNNNNNNGC 1 cut(s) 571
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 440
BstV1I GCAGC 2 cut(s) 55, 426
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 2 cut(s) 746, 805
BstYI RGATCY 2 cut(s) 746, 805
BsuRI GGCC 2 cut(s) 60, 552
BtsCI GGATG 6 cut(s) 263, 464, 865, 1168, 1173, 1339
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 288, 546, 735
Cac8I GCNNGC 1 cut(s) 1171
CciI TCATGA 2 cut(s) 1192, 1339
CfoI GCGC 1 cut(s) 743
Cfr13I GGNCC 4 cut(s) 50, 58, 169, 968
Csp6I GTAC 4 cut(s) 16, 207, 361, 733
CviQI GTAC 4 cut(s) 16, 207, 361, 733
DdeI CTNAG 7 cut(s) 9, 80, 561, 683, 737, 765, 1309
DpnI GATC 7 cut(s) 278, 447, 748, 807, 1040, 1233, 1338
DpnII GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
DraIII CACNNNGTG 2 cut(s) 100, 686
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 513, 596, 1365
EarI CTCTTC 3 cut(s) 513, 596, 1365
Ecl136II GAGCTC 1 cut(s) 1397
Eco24I GRGCYC 1 cut(s) 1399
Eco31I GGTCTC 1 cut(s) 1295
Eco47I GGWCC 3 cut(s) 50, 169, 968
Eco53kI GAGCTC 1 cut(s) 1397
Eco57I CTGAAG 1 cut(s) 1346
EcoICRI GAGCTC 1 cut(s) 1397
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO109I RGGNCCY 2 cut(s) 169, 968
EcoRII CCWGG 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 1399
FalI AAGNNNNNCTT 2 cut(s) 1344, 1376
FaqI GGGAC 1 cut(s) 811
FbaI TGATCA 1 cut(s) 1336
Fnu4HI GCNGC 2 cut(s) 69, 440
FokI GGATG 6 cut(s) 250, 471, 872, 1160, 1175, 1346
FriOI GRGCYC 1 cut(s) 1399
Fsp4HI GCNGC 2 cut(s) 69, 440
FspBI CTAG 7 cut(s) 192, 204, 270, 417, 567, 710, 1032
GlaI GCGC 1 cut(s) 742
GluI GCNGC 2 cut(s) 69, 440
HaeIII GGCC 2 cut(s) 60, 552
HapII CCGG 1 cut(s) 1095
HhaI GCGC 1 cut(s) 743
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HinfI GANTC 8 cut(s) 530, 593, 635, 824, 1061, 1189, 1325, 1366
HpaII CCGG 1 cut(s) 1095
Hpy166II GTNNAC 5 cut(s) 232, 288, 733, 815, 1370
Hpy188I TCNGA 5 cut(s) 10, 766, 1188, 1300, 1365
Hpy188III TCNNGA 7 cut(s) 368, 833, 1095, 1193, 1340, 1352, 1400
Hpy8I GTNNAC 5 cut(s) 232, 288, 733, 815, 1370
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 9 cut(s) 83, 174, 307, 324, 374, 585, 839, 997, 1017
HpyCH4III ACNGT 3 cut(s) 54, 236, 620
HpyCH4V TGCA 9 cut(s) 24, 71, 442, 493, 616, 878, 897, 1132, 1202
HpyF10VI GCNNNNNNNGC 1 cut(s) 571
HpyF3I CTNAG 7 cut(s) 9, 80, 561, 683, 737, 765, 1309
HspAI GCGC 1 cut(s) 741
Kpn2I TCCGGA 1 cut(s) 1094
KpnI GGTACC 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 1336
Kzo9I GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
LguI GCTCTTC 1 cut(s) 1365
LmnI GCTCC 3 cut(s) 706, 1009, 1394
Lsp1109I GCAGC 2 cut(s) 55, 426
LweI GCATC 3 cut(s) 449, 1119, 1182
MaeI CTAG 7 cut(s) 192, 204, 270, 417, 567, 710, 1032
MaeIII GTNAC 6 cut(s) 92, 377, 451, 620, 1062, 1217
MalI GATC 7 cut(s) 278, 447, 748, 807, 1040, 1233, 1338
MboI GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
MboII GAAGA 7 cut(s) 508, 511, 530, 613, 800, 848, 1352
MflI RGATCY 2 cut(s) 746, 805
MhlI GDGCHC 2 cut(s) 984, 1399
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 4 cut(s) 39, 211, 873, 1294
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 4 cut(s) 833, 1070, 1183, 1375
MmeI TCCRAC 2 cut(s) 90, 652
Mox20I TGGCCA 1 cut(s) 552
MroI TCCGGA 1 cut(s) 1094
MroXI GAANNNNTTC 1 cut(s) 592
MscI TGGCCA 1 cut(s) 552
MseI TTAA 6 cut(s) 254, 309, 392, 483, 582, 1140
MslI CAYNNNNRTG 1 cut(s) 701
Msp20I TGGCCA 1 cut(s) 552
MspI CCGG 1 cut(s) 1095
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 2 cut(s) 441, 1026
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 571
NdeII GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
NlaIV GGNNCC 5 cut(s) 17, 171, 969, 1049, 1244
NmuCI GTSAC 5 cut(s) 92, 377, 620, 1062, 1217
PagI TCATGA 2 cut(s) 1192, 1339
PciSI GCTCTTC 1 cut(s) 1365
PctI GAATGC 2 cut(s) 441, 1026
PdmI GAANNNNTTC 1 cut(s) 592
PfeI GAWTC 4 cut(s) 530, 593, 635, 1325
PkrI GCNGC 2 cut(s) 70, 441
PleI GAGTC 4 cut(s) 832, 1069, 1183, 1374
PpsI GAGTC 4 cut(s) 832, 1069, 1183, 1374
PpuMI RGGWCCY 2 cut(s) 169, 968
Psp124BI GAGCTC 1 cut(s) 1399
Psp5II RGGWCCY 2 cut(s) 169, 968
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 5 cut(s) 17, 171, 969, 1049, 1244
PspPI GGNCC 4 cut(s) 50, 58, 169, 968
PspPPI RGGWCCY 2 cut(s) 169, 968
PstI CTGCAG 1 cut(s) 444
PsuI RGATCY 2 cut(s) 746, 805
RsaI GTAC 4 cut(s) 17, 208, 362, 734
RsaNI GTAC 4 cut(s) 16, 207, 361, 733
RseI CAYNNNNRTG 1 cut(s) 701
SacI GAGCTC 1 cut(s) 1399
SapI GCTCTTC 1 cut(s) 1365
SaqAI TTAA 6 cut(s) 254, 309, 392, 483, 582, 1140
SatI GCNGC 2 cut(s) 69, 440
Sau3AI GATC 7 cut(s) 276, 445, 746, 805, 1038, 1231, 1336
Sau96I GGNCC 4 cut(s) 50, 58, 169, 968
ScaI AGTACT 1 cut(s) 208
SchI GAGTC 4 cut(s) 833, 1070, 1183, 1375
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 2 cut(s) 984, 1399
SfaNI GCATC 3 cut(s) 449, 1119, 1182
SfcI CTRYAG 1 cut(s) 440
SinI GGWCC 3 cut(s) 50, 169, 968
SmiMI CAYNNNNRTG 1 cut(s) 701
SmlI CTYRAG 3 cut(s) 1156, 1288, 1398
SmoI CTYRAG 3 cut(s) 1156, 1288, 1398
SpeI ACTAGT 2 cut(s) 191, 203
Sse9I AATT 4 cut(s) 39, 211, 873, 1294
SsiI CCGC 1 cut(s) 649
SspMI CTAG 7 cut(s) 192, 204, 270, 417, 567, 710, 1032
SstI GAGCTC 1 cut(s) 1399
StyD4I CCNGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 54, 236, 620
TasI AATT 4 cut(s) 39, 211, 873, 1294
TatI WGTACW 1 cut(s) 206
TfiI GAWTC 4 cut(s) 530, 593, 635, 1325
Tru1I TTAA 6 cut(s) 254, 309, 392, 483, 582, 1140
Tru9I TTAA 6 cut(s) 254, 309, 392, 483, 582, 1140
TscAI CASTG 3 cut(s) 295, 553, 735
TseFI GTSAC 5 cut(s) 92, 377, 620, 1062, 1217
TseI GCWGC 2 cut(s) 68, 439
Tsp45I GTSAC 5 cut(s) 92, 377, 620, 1062, 1217
TspDTI ATGAA 3 cut(s) 585, 767, 897
TspRI CASTG 3 cut(s) 295, 553, 735
VpaK11BI GGWCC 3 cut(s) 50, 169, 968
XagI CCTNNNNNAGG 1 cut(s) 253
XapI RAATTY 1 cut(s) 1294
XcmI CCANNNNNNNNNTGG 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 592
XspI CTAG 7 cut(s) 192, 204, 270, 417, 567, 710, 1032
ZrmI AGTACT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.