MD14G1206500.v1.1

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011359
Physical Location & Seq
Reverse (-)
29366077 .. 29370435
4359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1206500.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGGCTTCTGAAACTTACCAATCCAAAAATGTATGCGTGATTGGAGCTGGACCCTCAGGGCTTGTAGCTGCAAGGGAGCTGAGGAAAGAAGGTCACAGTGTGGTGGTGTTGGAACAAAACCATGATGTGGGAGGGCAGTGGCTGTACGACCCAAATGTGGAGGGAGAGGATCCCTTAGGAAGGTCACCTACCCTAAAAGTGCATAGCAGTCTTTACACCTCTTTGAGGCTCATATCTCCAAGAGAGATTATGGGGTTCACTGACTTTCCATTTGCAGTCAAGAAAGGTAGGGACATGAGGAGGTTTCCTGGCCATACGGAGCTTCTTTTGTACCTCAAGGATTTTTGTGATTGGTTTGGGTTGAGAGAGTTGATAAGGTTCAATACTAGGGTTTCATATGTGGGGATGTTGAATGGAGATCATGTAGTTGGTTGCAAAGATCTGAAATGGGTTGTGAAGAGTGTGGAGAATAAGACCGAGAGTTTCGTGGAGGAGGTGTTTGATGCGGTGGTTGTGGCCACCGGTCATTACTCTAAGCCAAGGTTGCCCTCCATTAAAGGAATGGATGCATGGAAGAGGAAGCAAATGCATAGTCACATTTACAGGGTTCCGGAGCCATTTCGTGATGAGGTTGTGGTGGTGGTTGGAACTTCACTAAGTGGGCAAGATATATCAATGGAACTAGTGGATGTGGCAAAGGCAATCTACCTCAGTGGCAAATCTCTTGACATATCTGAGGGCTTGTCCAAAGTCATTACCAAACATGAAAACTGGCACCTTCGTCCCCAGATAGATGTACTTCAAGAAGACGGGAAGGTGTTATTTGTAGACGGTTCTTGGATCATTGCAGACACTATCATATATTGTACTGGGTATTCATACACATTCCCCTTTCTTGACACCAAAGGAATAGTAGCTGTGGATGATGACAGAGTGGGGCCTTTGTATGAGCATACCTTCCCTCCATCGCTTGCTCCCTTTTTATCATTTGTAGGCATTCCTAGAAAGATCATAGGGTTTCCTTTTTTTGAGTCACAAGCAAAATGGACAGCTCAACTGCTCTCTGGCAAAACAACATTGCCATCAAGGGATGACATGATGCAGTCAATCAAGGAGTTCTACCACTCAAGGGATGTTGCTGGCATTCCGAAGCATAACACCCACGACATCGCCGAGTTCGAGTATTGTGATAGATATGGGGATCACATAGGGTTCCCACATTTGGAAGAATGGAGGAAAGAGCTTTGCCTTTCGGCACTAAGAAATGCTGACACCGACTTAGAAACATATAGGGATTCGTGGGATGATCATGATCTGCTTCAAGAGGCTCTTCAAAGTCCACATTTTACTCAACTTGGGCCTCAAGATTTTGATTTTCCTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like K_oxygenase

Protein Analysis

462

Amino Acids

52.41

Weight (kDa)

5.57

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 5 - 47 4.5e-06 Thi4 family
FMO-like PF00743 9 - 241 1.1e-32 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 10 - 299 1.3e-16 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 10 - 44 8.4e-06 HI0933-like protein Rossmann domain
FAD_binding_3 PF01494 10 - 41 5.3e-06 FAD binding domain
DAO PF01266 11 - 45 2.8e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 13 - 47 1.5e-10 NAD(P)-binding Rossmann-like domain
Pyr_redox_3 PF13738 73 - 313 2.4e-13 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 260 - 387 3.2e-15 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07800
fragaria_vesca FvH4_5g14390
malus_domestica MD06G1199100.v1.1 MD14G1206500.v1.1 MD14G1206800.v1.1 MD14G1207200.v1.1
prunus_persica Prupe.5G199900_v2.0.a1
pyrus_communis pycom14g17100 pycom14g17110 pycom14g17120
rosa_chinensis RchiOBHm_Chr7g0182301
rosa_laevigata RLG00000005134
rosa_multiflora Rmu_sc0000003.1_g000007
rosa_roxburghii Rroxscaffold_3G00271340
rosa_rugosa Rorug06G0446900
rosa_samantha Rh7AG049800 Rh7BG049900 Rh7CG051200 Rh7DG049100
rosa_wichuraiana Rw7G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 774
AccB7I CCANNNNNTGG 1 cut(s) 127
AccI GTMKAC 1 cut(s) 828
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 1 cut(s) 506
AclWI GGATC 4 cut(s) 164, 177, 848, 1209
AcoI YGGCCR 2 cut(s) 310, 516
AdeI CACNNNGTG 2 cut(s) 100, 659
AfaI GTAC 4 cut(s) 146, 332, 798, 868
AfiI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 5 cut(s) 382, 412, 803, 1322, 1334
AhlI ACTAGT 1 cut(s) 682
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 7 cut(s) 47, 68, 79, 322, 917, 1052, 1243
AluI AGCT 7 cut(s) 47, 68, 79, 322, 917, 1052, 1243
AlwI GGATC 4 cut(s) 164, 177, 848, 1209
AlwNI CAGNNNCTG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 4 cut(s) 310, 516, 938, 1358
ApeKI GCWGC 1 cut(s) 68
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 577, 609
AsiGI ACCGGT 1 cut(s) 521
AspS9I GGNCC 3 cut(s) 50, 938, 1358
AsuHPI GGTGA 1 cut(s) 177
AvaII GGWCC 1 cut(s) 50
AxyI CCTNAGG 2 cut(s) 55, 175
BalI TGGCCA 2 cut(s) 312, 518
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 774
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
BbsI GAAGAC 1 cut(s) 813
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 55
BccI CCATC 2 cut(s) 973, 1090
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 1 cut(s) 1306
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 3 cut(s) 387, 683, 1002
BglII AGATCT 1 cut(s) 439
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 3 cut(s) 50, 938, 1358
BmiI GGNNCC 7 cut(s) 52, 171, 609, 615, 776, 939, 1214
BmrFI CCNGG 1 cut(s) 309
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 3 cut(s) 493, 556, 1089
BmuI ACTGGG 1 cut(s) 879
BpiI GAAGAC 1 cut(s) 813
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 3 cut(s) 320, 1111, 1347
BsaBI GATNNNNATC 1 cut(s) 1311
BsaJI CCNNGG 1 cut(s) 539
BsaWI WCCGGW 2 cut(s) 521, 610
BsaXI ACNNNNNCTCC 6 cut(s) 408, 438, 482, 512, 946, 976
Bsc4I CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 2 cut(s) 776, 874
Bse21I CCTNAGG 2 cut(s) 55, 175
Bse3DI GCAATG 2 cut(s) 843, 1076
Bse8I GATNNNNATC 1 cut(s) 1311
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 7 cut(s) 411, 571, 694, 928, 1096, 1138, 1309
BseJI GATNNNNATC 1 cut(s) 1311
BseLI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
BseMI GCAATG 2 cut(s) 843, 1076
BseMII CTCAG 4 cut(s) 69, 71, 724, 726
BseNI ACTGG 2 cut(s) 776, 874
BseRI GAGGAG 2 cut(s) 313, 506
BseXI GCAGC 1 cut(s) 55
BshFI GGCC 4 cut(s) 312, 518, 940, 1360
BshNI GGYRCC 1 cut(s) 774
BshTI ACCGGT 1 cut(s) 521
BsiSI CCGG 2 cut(s) 522, 611
BslFI GGGAC 2 cut(s) 305, 768
BslI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
BsmFI GGGAC 2 cut(s) 305, 768
BsmI GAATGC 2 cut(s) 996, 1143
BsnI GGCC 4 cut(s) 312, 518, 940, 1360
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
BspACI CCGC 1 cut(s) 506
BspANI GGCC 4 cut(s) 312, 518, 940, 1360
BspCNI CTCAG 4 cut(s) 68, 72, 723, 727
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 1309
BspLI GGNNCC 7 cut(s) 52, 171, 609, 615, 776, 939, 1214
BspPI GGATC 4 cut(s) 164, 177, 848, 1209
BspQI GCTCTTC 1 cut(s) 1335
BspT107I GGYRCC 1 cut(s) 774
BsrDI GCAATG 2 cut(s) 843, 1076
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 2 cut(s) 776, 874
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
BssT1I CCWWGG 1 cut(s) 539
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 98, 833
Bst6I CTCTTC 3 cut(s) 452, 569, 1335
BstC8I GCNNGC 2 cut(s) 972, 1141
BstDEI CTNAG 9 cut(s) 55, 80, 175, 534, 656, 710, 735, 1259, 1279
BstEII GGTNACC 1 cut(s) 183
BstENI CCTNNNNNAGG 2 cut(s) 178, 223
BstF5I GGATG 7 cut(s) 411, 571, 694, 928, 1096, 1138, 1309
BstKTI GATC 8 cut(s) 172, 421, 442, 843, 1011, 1204, 1309, 1315
BstMBI GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 1 cut(s) 309
BstPI GGTNACC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 307
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 813
BstX2I RGATCY 2 cut(s) 169, 439
BstYI RGATCY 2 cut(s) 169, 439
Bsu36I CCTNAGG 2 cut(s) 55, 175
BsuRI GGCC 4 cut(s) 312, 518, 940, 1360
BtgZI GCGATG 2 cut(s) 951, 1153
BtsCI GGATG 7 cut(s) 411, 571, 694, 928, 1096, 1138, 1309
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 4 cut(s) 103, 143, 258, 718
Cac8I GCNNGC 2 cut(s) 972, 1141
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 1309
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 3 cut(s) 50, 938, 1358
Csp6I GTAC 4 cut(s) 145, 331, 797, 867
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 7 cut(s) 122, 295, 422, 570, 764, 1096, 1310
CviQI GTAC 4 cut(s) 145, 331, 797, 867
DdeI CTNAG 9 cut(s) 55, 80, 175, 534, 656, 710, 735, 1259, 1279
DpnI GATC 8 cut(s) 171, 420, 441, 842, 1010, 1203, 1308, 1314
DpnII GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
DraIII CACNNNGTG 2 cut(s) 100, 659
EaeI YGGCCR 2 cut(s) 310, 516
Eam1104I CTCTTC 3 cut(s) 452, 569, 1335
EarI CTCTTC 3 cut(s) 452, 569, 1335
Eco130I CCWWGG 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 50
Eco81I CCTNAGG 2 cut(s) 55, 175
Eco91I GGTNACC 1 cut(s) 183
EcoNI CCTNNNNNAGG 2 cut(s) 178, 223
EcoO109I RGGNCCY 1 cut(s) 938
EcoO65I GGTNACC 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 539
EcoT22I ATGCAT 2 cut(s) 571, 591
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 7 cut(s) 125, 298, 425, 573, 767, 1099, 1313
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 2 cut(s) 305, 768
FatI CATG 7 cut(s) 121, 294, 421, 569, 763, 1095, 1309
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 1306
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 7 cut(s) 418, 578, 701, 935, 1103, 1145, 1316
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 3 cut(s) 387, 683, 1002
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 4 cut(s) 312, 518, 940, 1360
HapII CCGG 2 cut(s) 522, 611
Hin1II CATG 7 cut(s) 125, 298, 425, 573, 767, 1099, 1313
HinfI GANTC 2 cut(s) 1031, 1295
HpaII CCGG 2 cut(s) 522, 611
HphI GGTGA 1 cut(s) 177
Hpy166II GTNNAC 3 cut(s) 258, 829, 1340
Hpy188I TCNGA 4 cut(s) 10, 444, 736, 1149
Hpy188III TCNNGA 9 cut(s) 280, 611, 623, 725, 803, 896, 1310, 1322, 1364
Hpy8I GTNNAC 3 cut(s) 258, 829, 1340
HpyAV CCTTC 5 cut(s) 83, 174, 788, 808, 967
HpyCH4III ACNGT 2 cut(s) 98, 833
HpyCH4V TGCA 8 cut(s) 71, 202, 275, 435, 569, 589, 848, 1102
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 9 cut(s) 55, 80, 175, 534, 656, 710, 735, 1259, 1279
Hsp92II CATG 7 cut(s) 125, 298, 425, 573, 767, 1099, 1313
Kpn2I TCCGGA 1 cut(s) 610
Ksp22I TGATCA 1 cut(s) 1306
Kzo9I GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 5 cut(s) 44, 76, 319, 613, 979
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 3 cut(s) 493, 556, 1089
MaeI CTAG 3 cut(s) 387, 683, 1002
MaeIII GTNAC 4 cut(s) 92, 183, 593, 1032
MalI GATC 8 cut(s) 171, 420, 441, 842, 1010, 1203, 1308, 1314
MboI GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
MboII GAAGA 5 cut(s) 469, 586, 818, 1238, 1322
MflI RGATCY 2 cut(s) 169, 439
MlsI TGGCCA 2 cut(s) 312, 518
MluNI TGGCCA 2 cut(s) 312, 518
MlyI GAGTC 1 cut(s) 1040
MmeI TCCRAC 2 cut(s) 90, 625
Mox20I TGGCCA 2 cut(s) 312, 518
Mph1103I ATGCAT 2 cut(s) 571, 591
MroI TCCGGA 1 cut(s) 610
MscI TGGCCA 2 cut(s) 312, 518
MseI TTAA 2 cut(s) 555, 1384
Msp20I TGGCCA 2 cut(s) 312, 518
MspI CCGG 2 cut(s) 522, 611
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 996, 1143
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 544
NdeI CATATG 1 cut(s) 397
NdeII GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
NlaIII CATG 7 cut(s) 125, 298, 425, 573, 767, 1099, 1313
NlaIV GGNNCC 7 cut(s) 52, 171, 609, 615, 776, 939, 1214
NmeAIII GCCGAG 1 cut(s) 1198
NmuCI GTSAC 4 cut(s) 92, 183, 593, 1032
NsiI ATGCAT 2 cut(s) 571, 591
PagI TCATGA 1 cut(s) 1309
PciSI GCTCTTC 1 cut(s) 1335
PcsI WCGNNNNNNNCGW 2 cut(s) 1170, 1176
PctI GAATGC 2 cut(s) 996, 1143
PfeI GAWTC 1 cut(s) 1295
PflMI CCANNNNNTGG 1 cut(s) 127
PinAI ACCGGT 1 cut(s) 521
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 1 cut(s) 1039
PpsI GAGTC 1 cut(s) 1039
Psp6I CCWGG 1 cut(s) 307
PspEI GGTNACC 1 cut(s) 183
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 7 cut(s) 52, 171, 609, 615, 776, 939, 1214
PspPI GGNCC 3 cut(s) 50, 938, 1358
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 2 cut(s) 169, 439
RsaI GTAC 4 cut(s) 146, 332, 798, 868
RsaNI GTAC 4 cut(s) 145, 331, 797, 867
SapI GCTCTTC 1 cut(s) 1335
SaqAI TTAA 2 cut(s) 555, 1384
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 8 cut(s) 169, 418, 439, 840, 1008, 1201, 1306, 1312
Sau96I GGNCC 3 cut(s) 50, 938, 1358
SchI GAGTC 1 cut(s) 1040
ScrFI CCNGG 1 cut(s) 309
SfaNI GCATC 3 cut(s) 493, 556, 1089
SinI GGWCC 1 cut(s) 50
SmlI CTYRAG 3 cut(s) 335, 1126, 1362
SmoI CTYRAG 3 cut(s) 335, 1126, 1362
SpeI ACTAGT 1 cut(s) 682
SsiI CCGC 1 cut(s) 506
SspMI CTAG 3 cut(s) 387, 683, 1002
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 2 cut(s) 98, 833
TaqI TCGA 1 cut(s) 1179
TaqII GACCGA 1 cut(s) 491
TatI WGTACW 2 cut(s) 796, 866
TfiI GAWTC 1 cut(s) 1295
Tru1I TTAA 2 cut(s) 555, 1384
Tru9I TTAA 2 cut(s) 555, 1384
TscAI CASTG 4 cut(s) 103, 143, 265, 718
TseFI GTSAC 4 cut(s) 92, 183, 593, 1032
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 4 cut(s) 92, 183, 593, 1032
TspDTI ATGAA 3 cut(s) 384, 780, 867
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 4 cut(s) 103, 143, 265, 718
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 50
XagI CCTNNNNNAGG 2 cut(s) 178, 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 3 cut(s) 387, 683, 1002
Zsp2I ATGCAT 2 cut(s) 571, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.