MD06G1199100.v1.1

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011359
Physical Location & Seq
Reverse (-)
33294285 .. 33297004
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1199100.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGGTTTCTGAAACTTACCGATCCAAAAATGTCTGTGTGATTGGAGCTGGACCCTCAGGGCTTGTAGCTGCAAGGGAGCTGAGGAAGGAAGGTCACAAGGTGGTGGTGTTGGAACAAAACCATGATGTGGGAGGGCAGTGGCTGTACGACCCAAATGTGGAGGGAGAGGATCCCTTAGGAAGGGTACCTACCCTAAAAGTGCATAGCAGTCTTTACACCTCTTTGAGGCTCATATCTCCAAGAGAGATCATGGGGTTCACTGACTTTCCATTTGCAGTAAAAAAAGGTAGGGACATGAGGAGGTTTCCGGGCCATAGGGAACTTCTATTGTACCTCAAGGATTTTTGTGATTGGTTTGGGTTGAGGGAGTTGATAAGGTTCAATACTAGGGTTTCATATGTGGGGATGTTGGATGGAGATCATGTAGTTGGTTGCAAAGATTTGAAATGGGTTGTGAAGAGTGTGGAAAAGAAGACCGAGATTTTCATAGAAGAGGTGTTTGATGCGGTGGTTGTGGCCACGGGCCACTACTCTAAGCCAAGGTTGCCATCCATTCAAGGAATGGATGCATGGAAAAGGAAGCAACTGCATAGTCACATCTACAGGGTTCCGGAGCCATTTCGTGATGAGGTTGTGGTGGTGGTTGGGACTTCACTAAGCGGACAAGATATATCAATGGAACTAGTGGATGTGGCGAAGGCAATCTACCTCAGTGGAAAATCTCTTGACATATCTGAGGGCTTGTCCAAAGTCATTTCCAAACACGAAAACTTGCACCTTCGTCCCCAGATAGAGGCACTTCAAGAAGATGGGAAGGTATTATTTGTGGACGGTTCTTGGGTCATTGCAGACACTATCATGTACTGCACTGGGTATTCATACACATTCCCCTTTCTTGACACCAAAGGAATAGTAACTGTGGATGATGACAGAGTGAGCCCCTTGTATGAGCACACCTTCCCTCCATCACTTGCTCCCTCTTTATCTTTTATAGGCATTCCTAGAAAGATAATAGGGTTCCCTTTCTTTGAGTCACAAGCAAAATGGATAGCTCAACTGCTCTCTGGGAAAACAACATTGCCATCAAGGGATGACATGATGCAGTCCATCAAGGAGTTCTACCACTCAAGGGATGTTGCTGGCATTCCAAAGCATAACACCCACGACATCGCCGAGTTTGAGTATTGTGACAAATATGGAGATCATATAGGGTTCCCACATTTGGAAGAATGGAGGAAAGAGCTTTGCCTTTCGGCACTAAGAAATGCTGAAACCGACTTGGAAACGTATAGGGATTCGTGGGATGATCATGAGCTGCTTCAAGAGGCTCTTCAAAGTCCACATTTTACTCAACTTGGGGCTCAAGATTTAGATTTTCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like K_oxygenase

Protein Analysis

462

Amino Acids

52.48

Weight (kDa)

5.72

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 5 - 47 7.3e-07 Thi4 family
FMO-like PF00743 9 - 241 2.4e-32 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 10 - 303 1.9e-16 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 10 - 44 5.1e-06 HI0933-like protein Rossmann domain
FAD_binding_3 PF01494 10 - 41 5.4e-06 FAD binding domain
DAO PF01266 11 - 45 2.7e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 13 - 47 1.3e-10 NAD(P)-binding Rossmann-like domain
Pyr_redox_3 PF13738 73 - 317 3.1e-13 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 258 - 397 1.1e-16 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07800
fragaria_vesca FvH4_5g14390
malus_domestica MD06G1199100.v1.1 MD14G1206500.v1.1 MD14G1206800.v1.1 MD14G1207200.v1.1
prunus_persica Prupe.5G199900_v2.0.a1
pyrus_communis pycom14g17100 pycom14g17110 pycom14g17120
rosa_chinensis RchiOBHm_Chr7g0182301
rosa_laevigata RLG00000005134
rosa_multiflora Rmu_sc0000003.1_g000007
rosa_roxburghii Rroxscaffold_3G00271340
rosa_rugosa Rorug06G0446900
rosa_samantha Rh7AG049800 Rh7BG049900 Rh7CG051200 Rh7DG049100
rosa_wichuraiana Rw7G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 184
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 127
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 2 cut(s) 506, 660
AclWI GGATC 3 cut(s) 15, 164, 177
AcoI YGGCCR 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 4 cut(s) 146, 186, 332, 863
AfiI CCNNNNNNNGG 7 cut(s) 127, 157, 180, 225, 793, 1129, 1222
AgsI TTSAA 6 cut(s) 382, 445, 557, 803, 1322, 1334
AhlI ACTAGT 1 cut(s) 682
AluBI AGCT 6 cut(s) 47, 68, 79, 1052, 1243, 1315
AluI AGCT 6 cut(s) 47, 68, 79, 1052, 1243, 1315
Alw21I GWGCWC 1 cut(s) 954
AlwI GGATC 3 cut(s) 15, 164, 177
AlwNI CAGNNNCTG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 3 cut(s) 310, 516, 523
ApeKI GCWGC 2 cut(s) 68, 1315
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
Asp718I GGTACC 1 cut(s) 184
AspS9I GGNCC 3 cut(s) 50, 310, 523
AsuC2I CCSGG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 50
AxyI CCTNAGG 2 cut(s) 55, 175
BalI TGGCCA 1 cut(s) 518
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 2 cut(s) 941, 1363
BarI GAAGNNNNNNTAC 4 cut(s) 172, 204, 689, 721
BbsI GAAGAC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 954
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 55, 1302
BccI CCATC 6 cut(s) 407, 556, 803, 973, 1090, 1115
BclI TGATCA 1 cut(s) 1306
BcnI CCSGG 1 cut(s) 309
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 3 cut(s) 387, 683, 1002
BfmI CTRYAG 1 cut(s) 601
BisI GCNGC 2 cut(s) 69, 1316
BlsI GCNGC 2 cut(s) 70, 1317
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 3 cut(s) 50, 310, 523
BmiI GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 1019, 1214
BmrFI CCNGG 1 cut(s) 309
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 3 cut(s) 493, 556, 1089
BmuI ACTGGG 1 cut(s) 879
BpiI GAAGAC 1 cut(s) 479
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 3 cut(s) 320, 1111, 1347
BpuMI CCSGG 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 2 cut(s) 519, 539
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 946, 976
Bsc4I CCNNNNNNNGG 7 cut(s) 127, 157, 180, 225, 793, 1129, 1222
Bse1I ACTGG 1 cut(s) 874
Bse21I CCTNAGG 2 cut(s) 55, 175
Bse3DI GCAATG 2 cut(s) 843, 1076
Bse8I GATNNNNATC 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 610
BseDI CCNNGG 2 cut(s) 519, 539
BseGI GGATG 9 cut(s) 411, 418, 548, 571, 694, 928, 1096, 1138, 1309
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 7 cut(s) 127, 157, 180, 225, 793, 1129, 1222
BseMI GCAATG 2 cut(s) 843, 1076
BseMII CTCAG 4 cut(s) 69, 71, 724, 726
BseNI ACTGG 1 cut(s) 874
BseRI GAGGAG 1 cut(s) 313
BseXI GCAGC 2 cut(s) 55, 1302
BsgI GTGCAG 1 cut(s) 850
BshFI GGCC 3 cut(s) 312, 518, 525
BshNI GGYRCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 954
BsiSI CCGG 2 cut(s) 308, 611
BslFI GGGAC 3 cut(s) 305, 661, 768
BslI CCNNNNNNNGG 7 cut(s) 127, 157, 180, 225, 793, 1129, 1222
BsmFI GGGAC 3 cut(s) 305, 661, 768
BsmI GAATGC 2 cut(s) 996, 1143
BsnI GGCC 3 cut(s) 312, 518, 525
Bsp1286I GDGCHC 3 cut(s) 941, 954, 1363
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
BspACI CCGC 2 cut(s) 506, 660
BspANI GGCC 3 cut(s) 312, 518, 525
BspCNI CTCAG 4 cut(s) 68, 72, 723, 727
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 1309
BspLI GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 1019, 1214
BspPI GGATC 3 cut(s) 15, 164, 177
BspQI GCTCTTC 1 cut(s) 1335
BspT107I GGYRCC 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 843, 1076
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 2 cut(s) 519, 539
BssMI GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
BssT1I CCWWGG 1 cut(s) 539
Bst4CI ACNGT 2 cut(s) 833, 919
Bst6I CTCTTC 3 cut(s) 452, 486, 1335
BstC8I GCNNGC 1 cut(s) 1141
BstDEI CTNAG 8 cut(s) 55, 80, 175, 534, 656, 710, 735, 1259
BstDSI CCRYGG 1 cut(s) 519
BstENI CCTNNNNNAGG 2 cut(s) 178, 223
BstF5I GGATG 9 cut(s) 411, 418, 548, 571, 694, 928, 1096, 1138, 1309
BstKTI GATC 6 cut(s) 23, 172, 249, 421, 1204, 1309
BstMBI GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 601
BstV1I GCAGC 2 cut(s) 55, 1302
BstV2I GAAGAC 1 cut(s) 479
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
Bsu36I CCTNAGG 2 cut(s) 55, 175
BsuRI GGCC 3 cut(s) 312, 518, 525
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 1 cut(s) 1153
BtsCI GGATG 9 cut(s) 411, 418, 548, 571, 694, 928, 1096, 1138, 1309
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 4 cut(s) 143, 258, 718, 867
Cac8I GCNNGC 1 cut(s) 1141
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 1309
Cfr13I GGNCC 3 cut(s) 50, 310, 523
Csp6I GTAC 4 cut(s) 145, 185, 331, 862
CviAII CATG 8 cut(s) 122, 250, 295, 422, 570, 859, 1096, 1310
CviQI GTAC 4 cut(s) 145, 185, 331, 862
DdeI CTNAG 8 cut(s) 55, 80, 175, 534, 656, 710, 735, 1259
DpnI GATC 6 cut(s) 22, 171, 248, 420, 1203, 1308
DpnII GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
DraIII CACNNNGTG 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 3 cut(s) 452, 486, 1335
EarI CTCTTC 3 cut(s) 452, 486, 1335
Eco130I CCWWGG 1 cut(s) 539
Eco24I GRGCYC 2 cut(s) 941, 1363
Eco47I GGWCC 1 cut(s) 50
Eco81I CCTNAGG 2 cut(s) 55, 175
EcoNI CCTNNNNNAGG 2 cut(s) 178, 223
EcoT14I CCWWGG 1 cut(s) 539
EcoT22I ATGCAT 1 cut(s) 571
EcoT38I GRGCYC 2 cut(s) 941, 1363
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 8 cut(s) 125, 253, 298, 425, 573, 862, 1099, 1313
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 3 cut(s) 305, 661, 768
FatI CATG 8 cut(s) 121, 249, 294, 421, 569, 858, 1095, 1309
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 1306
Fnu4HI GCNGC 2 cut(s) 69, 1316
FokI GGATG 9 cut(s) 418, 425, 535, 578, 701, 935, 1103, 1145, 1316
FriOI GRGCYC 2 cut(s) 941, 1363
Fsp4HI GCNGC 2 cut(s) 69, 1316
FspBI CTAG 3 cut(s) 387, 683, 1002
GluI GCNGC 2 cut(s) 69, 1316
HaeIII GGCC 3 cut(s) 312, 518, 525
HapII CCGG 2 cut(s) 308, 611
Hin1II CATG 8 cut(s) 125, 253, 298, 425, 573, 862, 1099, 1313
HinfI GANTC 2 cut(s) 1031, 1295
HpaII CCGG 2 cut(s) 308, 611
Hpy166II GTNNAC 3 cut(s) 258, 829, 1340
Hpy188I TCNGA 2 cut(s) 10, 736
Hpy188III TCNNGA 8 cut(s) 611, 623, 725, 803, 896, 1310, 1322, 1364
Hpy8I GTNNAC 3 cut(s) 258, 829, 1340
HpyAV CCTTC 7 cut(s) 79, 83, 174, 691, 788, 808, 967
HpyCH4III ACNGT 2 cut(s) 833, 919
HpyCH4IV ACGT 1 cut(s) 1286
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 8 cut(s) 55, 80, 175, 534, 656, 710, 735, 1259
HpySE526I ACGT 1 cut(s) 1286
Hsp92II CATG 8 cut(s) 125, 253, 298, 425, 573, 862, 1099, 1313
Kpn2I TCCGGA 1 cut(s) 610
KpnI GGTACC 1 cut(s) 188
Ksp22I TGATCA 1 cut(s) 1306
Kzo9I GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 4 cut(s) 44, 76, 613, 979
LpnPI CCDG 9 cut(s) 33, 42, 321, 589, 624, 800, 855, 1050, 1125
Lsp1109I GCAGC 2 cut(s) 55, 1302
LweI GCATC 3 cut(s) 493, 556, 1089
MaeI CTAG 3 cut(s) 387, 683, 1002
MaeII ACGT 1 cut(s) 1286
MaeIII GTNAC 5 cut(s) 92, 593, 913, 1032, 1187
MalI GATC 6 cut(s) 22, 171, 248, 420, 1203, 1308
MboI GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
MboII GAAGA 6 cut(s) 469, 484, 503, 818, 1238, 1322
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 3 cut(s) 941, 954, 1363
MlsI TGGCCA 1 cut(s) 518
MluNI TGGCCA 1 cut(s) 518
MlyI GAGTC 1 cut(s) 1040
MmeI TCCRAC 2 cut(s) 90, 390
Mox20I TGGCCA 1 cut(s) 518
Mph1103I ATGCAT 1 cut(s) 571
MroI TCCGGA 1 cut(s) 610
MscI TGGCCA 1 cut(s) 518
MslI CAYNNNNRTG 1 cut(s) 857
Msp20I TGGCCA 1 cut(s) 518
MspI CCGG 2 cut(s) 308, 611
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 996, 1143
MwoI GCNNNNNNNGC 1 cut(s) 544
NciI CCSGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 397
NdeII GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
NlaIII CATG 8 cut(s) 125, 253, 298, 425, 573, 862, 1099, 1313
NlaIV GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 1019, 1214
NmeAIII GCCGAG 1 cut(s) 1198
NmuCI GTSAC 4 cut(s) 92, 593, 1032, 1187
NsiI ATGCAT 1 cut(s) 571
PagI TCATGA 1 cut(s) 1309
PciSI GCTCTTC 1 cut(s) 1335
PcsI WCGNNNNNNNCGW 1 cut(s) 1170
PctI GAATGC 2 cut(s) 996, 1143
PfeI GAWTC 1 cut(s) 1295
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 2 cut(s) 70, 1317
PleI GAGTC 1 cut(s) 1039
PpsI GAGTC 1 cut(s) 1039
PspN4I GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 1019, 1214
PspPI GGNCC 3 cut(s) 50, 310, 523
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 4 cut(s) 146, 186, 332, 863
RsaNI GTAC 4 cut(s) 145, 185, 331, 862
RseI CAYNNNNRTG 1 cut(s) 857
SapI GCTCTTC 1 cut(s) 1335
SatI GCNGC 2 cut(s) 69, 1316
Sau3AI GATC 6 cut(s) 20, 169, 246, 418, 1201, 1306
Sau96I GGNCC 3 cut(s) 50, 310, 523
SchI GAGTC 1 cut(s) 1040
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 3 cut(s) 941, 954, 1363
SfaNI GCATC 3 cut(s) 493, 556, 1089
SfcI CTRYAG 1 cut(s) 601
SinI GGWCC 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 857
SmlI CTYRAG 3 cut(s) 335, 1126, 1362
SmoI CTYRAG 3 cut(s) 335, 1126, 1362
SpeI ACTAGT 1 cut(s) 682
SsiI CCGC 2 cut(s) 506, 660
SspMI CTAG 3 cut(s) 387, 683, 1002
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 2 cut(s) 833, 919
TaiI ACGT 1 cut(s) 1289
TaqII GACCGA 1 cut(s) 491
TatI WGTACW 1 cut(s) 861
TfiI GAWTC 1 cut(s) 1295
TscAI CASTG 4 cut(s) 143, 265, 718, 874
TseFI GTSAC 4 cut(s) 92, 593, 1032, 1187
TseI GCWGC 2 cut(s) 68, 1315
Tsp45I GTSAC 4 cut(s) 92, 593, 1032, 1187
TspDTI ATGAA 3 cut(s) 384, 475, 867
TspRI CASTG 4 cut(s) 143, 265, 718, 874
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 50
XagI CCTNNNNNAGG 2 cut(s) 178, 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XspI CTAG 3 cut(s) 387, 683, 1002
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.