Rroxscaffold_3G00271340

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011359
Physical Location & Seq
Forward (+)
63706791 .. 63709394
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271340.1

Sequence Viewer

Length: 1443 bp
ATGGTGTCTGAGAGGTACCAATCCAAAAGTGTGTGCGTAATTGGGGCTGGACCCTCAGGGCTTGTTGCTGCAAGAGAGCTGAGGAAAGAAGGTCACAGAGTGGTGGTGTTGGAACAAAACCATGATGTAGGAGGTCAATGGCTGTATGACCCAAATGTGGAGGGAGAGGACCCCTTAGGAAGGAACACTACTAGTCCTACTACTAGTACGAAGTCTTATCATCTAAAAGTCCACAGTAGTCTTTATACCTCTTTAAGGCTAACATCTCCAAGAGAGATCATGGGATTCACTGACTTCCCATTTTCAATTAAGAAGGGTAGAGACATGAGAAGGTTTCCTGGGCATAGGGAACTGCTTCTGTACCTTCAAGATTTTTGTGACTGGTTTGGGTTGAGGGATTTGATTAGGTTCAATACTAGGGTGGCTTATGTGGGCATGCTGGATCATGTAATGGGATGCCAAGATTTGAAATGGCTTGTTAAGAGTGTGGAGAAGAAGACCCAGATTGTGATGGAAGAGGTGTTTGATTCTGTGGTTATTGCCACCGGCCATTACTCTCAGCCTAGGTTGCCTTCCATTAATGGAATGGATTCATGGAAGAGGAAGCAAATGCACAGTCACATTTACAGGGTTCCCGAGCCCTTCCGTAATGAGGTTGTGGTTGTGGTTGGAACTTCACTAAGTGGGCAAGACATATCAATGGAGCTAGTAGAAGTGGCAAAGGCAGTGTACCTCAGCGCAAGATCGGTGAACAATATCTCTGAGGGCTTATCCAAGGTCATTTCCAAGCATGATACCTTGCATCTTCGTCCACAGATAGAGTCCCTTCAAGAAGATGGAAAGGTATTGTTTGTGGATGGTTCTTGGGTAACTGCAGACACCATCATCTACTGCACAGGGTATTCATACACATTCCCATTTCTTGACACCAAAGGAATAGTAGCTATTGATGATGACAGGGTGGGTCCTTTGTATGAGCACACCTTCCCTCCCTCACTTGCTCCTTCTTTATCTTTTATTGGCATTCCTAGACAGATCATAGGGTTCCCTTTCTTTGAGTCACAAGCAAAATGGATAGCTCAACTGCTCTCTGGGAAAAGAACATTGCCATCATGGGATGAAATGATGCAGTCCATTAAGGACTTCTACCACTCAAGGGATGTTGCTGGCATCCCAAATTACAATACTCATAATATTGCAGATTTCGAGTATTGCGACAAATATGGAGATCATGTCGGGTTCCCACATTTAGAGGAATGGAGGAAAGAGCTTTGCCTTTCTGCCTTAAGAAATTCTGAGACAAACTTAGAAACATATAGAGATTCGTTGGATGATCATGACTTGCTTCAAAGAGGCTCTTCAAAGTCCACATTTTACTCAACAAGAACAAGGAGCTCAAGATTTTCCTATGTAAAAGTCTTTAAATTATTCTTGTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like K_oxygenase

Protein Analysis

480

Amino Acids

54.9

Weight (kDa)

6.79

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 6 - 47 3.8e-06 Thi4 family
FMO-like PF00743 9 - 251 7.7e-29 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 10 - 308 1.1e-16 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 10 - 205 1.4e-07 HI0933-like protein Rossmann domain
FAD_binding_3 PF01494 10 - 41 3.7e-06 FAD binding domain
DAO PF01266 11 - 45 3.3e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 13 - 47 1.1e-10 NAD(P)-binding Rossmann-like domain
Pyr_redox_3 PF13738 176 - 325 8e-15 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 266 - 384 2.4e-16 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07800
fragaria_vesca FvH4_5g14390
malus_domestica MD06G1199100.v1.1 MD14G1206500.v1.1 MD14G1206800.v1.1 MD14G1207200.v1.1
prunus_persica Prupe.5G199900_v2.0.a1
pyrus_communis pycom14g17100 pycom14g17110 pycom14g17120
rosa_chinensis RchiOBHm_Chr7g0182301
rosa_laevigata RLG00000005134
rosa_multiflora Rmu_sc0000003.1_g000007
rosa_roxburghii Rroxscaffold_3G00271340
rosa_rugosa Rorug06G0446900
rosa_samantha Rh7AG049800 Rh7BG049900 Rh7CG051200 Rh7DG049100
rosa_wichuraiana Rw7G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 15
AccB1I GGYRCC 1 cut(s) 15
AclWI GGATC 1 cut(s) 450
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 1 cut(s) 1291
AdeI CACNNNGTG 2 cut(s) 100, 683
AfaI GTAC 4 cut(s) 17, 208, 362, 731
AfiI CCNNNNNNNGG 5 cut(s) 157, 180, 255, 652, 1156
AflII CTTAAG 1 cut(s) 1285
AgsI TTSAA 7 cut(s) 306, 368, 412, 469, 830, 1349, 1362
AhlI ACTAGT 2 cut(s) 191, 203
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 6 cut(s) 79, 706, 944, 1079, 1270, 1395
AluI AGCT 6 cut(s) 79, 706, 944, 1079, 1270, 1395
Alw21I GWGCWC 2 cut(s) 981, 1397
Alw26I GTCTC 2 cut(s) 315, 1292
AlwI GGATC 1 cut(s) 450
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 1 cut(s) 547
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 1 cut(s) 1291
ArsI GACNNNNNNTTYG 2 cut(s) 601, 633
AseI ATTAAT 1 cut(s) 579
Asp700I GAANNNNTTC 2 cut(s) 354, 589
Asp718I GGTACC 1 cut(s) 15
AspA2I CCTAGG 1 cut(s) 563
AspLEI GCGC 1 cut(s) 740
AspS9I GGNCC 3 cut(s) 50, 169, 965
AsuHPI GGTGA 1 cut(s) 760
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 3 cut(s) 50, 169, 965
AvrII CCTAGG 1 cut(s) 563
AxyI CCTNAGG 2 cut(s) 55, 175
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 2 cut(s) 642, 1397
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 2 cut(s) 981, 1397
BbvCI CCTCAGC 2 cut(s) 80, 734
BbvI GCAGC 1 cut(s) 55
BccI CCATC 5 cut(s) 505, 830, 851, 890, 1117
BciT130I CCWGG 1 cut(s) 339
BclI TGATCA 1 cut(s) 1333
BcoDI GTCTC 2 cut(s) 315, 1292
BcuI ACTAGT 2 cut(s) 191, 203
BfaI CTAG 6 cut(s) 192, 204, 417, 564, 707, 1029
BfmI CTRYAG 1 cut(s) 873
BfrI CTTAAG 1 cut(s) 1285
BisI GCNGC 1 cut(s) 69
BlnI CCTAGG 1 cut(s) 563
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 3 cut(s) 50, 169, 965
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 3 cut(s) 50, 169, 965
BmiI GGNNCC 7 cut(s) 17, 52, 171, 633, 966, 1046, 1241
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 4 cut(s) 446, 811, 1116, 1179
BpiI GAAGAC 1 cut(s) 503
Bpu10I CCTNAGC 2 cut(s) 80, 734
BpuEI CTTGAG 2 cut(s) 1138, 1381
BsaJI CCNNGG 3 cut(s) 338, 563, 774
BsaXI ACNNNNNCTCC 4 cut(s) 973, 1003, 1218, 1248
Bsc4I CCNNNNNNNGG 5 cut(s) 157, 180, 255, 652, 1156
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 1 cut(s) 386
Bse21I CCTNAGG 2 cut(s) 55, 175
Bse3DI GCAATG 1 cut(s) 1103
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 3 cut(s) 338, 563, 774
BseGI GGATG 6 cut(s) 461, 862, 1123, 1165, 1170, 1336
BseLI CCNNNNNNNGG 5 cut(s) 157, 180, 255, 652, 1156
BseMI GCAATG 1 cut(s) 1103
BseMII CTCAG 6 cut(s) 69, 71, 572, 748, 753, 1287
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 877
BshFI GGCC 1 cut(s) 549
BshNI GGYRCC 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 981, 1397
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 1 cut(s) 546
BslFI GGGAC 1 cut(s) 808
BslI CCNNNNNNNGG 5 cut(s) 157, 180, 255, 652, 1156
BsmAI GTCTC 2 cut(s) 315, 1292
BsmFI GGGAC 1 cut(s) 808
BsmI GAATGC 1 cut(s) 1023
BsnI GGCC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 642, 981, 1397
Bsp143I GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
BspANI GGCC 1 cut(s) 549
BspCNI CTCAG 6 cut(s) 68, 72, 571, 747, 754, 1288
BspHI TCATGA 1 cut(s) 1336
BspLI GGNNCC 7 cut(s) 17, 52, 171, 633, 966, 1046, 1241
BspMAI CTGCAG 1 cut(s) 877
BspPI GGATC 1 cut(s) 450
BspQI GCTCTTC 1 cut(s) 1363
BspT107I GGYRCC 1 cut(s) 15
BspTI CTTAAG 1 cut(s) 1285
BsrDI GCAATG 1 cut(s) 1103
BsrFI RCCGGY 1 cut(s) 545
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 3 cut(s) 338, 563, 774
BssMI GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
BssT1I CCWWGG 2 cut(s) 563, 774
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 2 cut(s) 236, 617
Bst6I CTCTTC 3 cut(s) 510, 593, 1363
BstAFI CTTAAG 1 cut(s) 1285
BstC8I GCNNGC 2 cut(s) 437, 1168
BstENI CCTNNNNNAGG 2 cut(s) 178, 253
BstF5I GGATG 6 cut(s) 461, 862, 1123, 1165, 1170, 1336
BstHHI GCGC 1 cut(s) 740
BstKTI GATC 6 cut(s) 279, 445, 746, 1038, 1231, 1336
BstMAI GTCTC 2 cut(s) 315, 1292
BstMBI GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
BstMWI GCNNNNNNNGC 1 cut(s) 568
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 873
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 503
Bsu36I CCTNAGG 2 cut(s) 55, 175
BsuRI GGCC 1 cut(s) 549
BtsCI GGATG 6 cut(s) 461, 862, 1123, 1165, 1170, 1336
BtsI GCAGTG 1 cut(s) 732
BtsIMutI CAGTG 2 cut(s) 288, 732
Cac8I GCNNGC 2 cut(s) 437, 1168
CciI TCATGA 1 cut(s) 1336
CfoI GCGC 1 cut(s) 740
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 3 cut(s) 50, 169, 965
Csp6I GTAC 4 cut(s) 16, 207, 361, 730
CviQI GTAC 4 cut(s) 16, 207, 361, 730
DpnI GATC 6 cut(s) 278, 444, 745, 1037, 1230, 1335
DpnII GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
DraI TTTAAA 1 cut(s) 1423
DraIII CACNNNGTG 2 cut(s) 100, 683
EaeI YGGCCR 1 cut(s) 547
Eam1104I CTCTTC 3 cut(s) 510, 593, 1363
EarI CTCTTC 3 cut(s) 510, 593, 1363
Ecl136II GAGCTC 1 cut(s) 1395
Eco130I CCWWGG 2 cut(s) 563, 774
Eco24I GRGCYC 2 cut(s) 642, 1397
Eco47I GGWCC 3 cut(s) 50, 169, 965
Eco53kI GAGCTC 1 cut(s) 1395
Eco81I CCTNAGG 2 cut(s) 55, 175
Eco88I CYCGRG 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 1395
EcoNI CCTNNNNNAGG 2 cut(s) 178, 253
EcoO109I RGGNCCY 2 cut(s) 169, 965
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 2 cut(s) 563, 774
EcoT38I GRGCYC 2 cut(s) 642, 1397
ErhI CCWWGG 2 cut(s) 563, 774
FalI AAGNNNNNCTT 2 cut(s) 1342, 1374
FaqI GGGAC 1 cut(s) 808
FbaI TGATCA 1 cut(s) 1333
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 6 cut(s) 468, 869, 1130, 1157, 1172, 1343
FriOI GRGCYC 2 cut(s) 642, 1397
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 6 cut(s) 192, 204, 417, 564, 707, 1029
GlaI GCGC 1 cut(s) 739
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 1 cut(s) 549
HapII CCGG 1 cut(s) 546
HhaI GCGC 1 cut(s) 740
Hin6I GCGC 1 cut(s) 738
HinP1I GCGC 1 cut(s) 738
HinfI GANTC 6 cut(s) 285, 527, 590, 821, 1058, 1322
HpaII CCGG 1 cut(s) 546
HphI GGTGA 1 cut(s) 760
Hpy166II GTNNAC 5 cut(s) 232, 730, 751, 812, 1368
Hpy188I TCNGA 3 cut(s) 10, 763, 1297
Hpy188III TCNNGA 6 cut(s) 368, 635, 830, 923, 1337, 1398
Hpy8I GTNNAC 5 cut(s) 232, 730, 751, 812, 1368
HpyCH4III ACNGT 2 cut(s) 236, 617
HpyCH4V TGCA 7 cut(s) 71, 613, 802, 875, 894, 1129, 1199
HpyF10VI GCNNNNNNNGC 1 cut(s) 568
HspAI GCGC 1 cut(s) 738
KpnI GGTACC 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 1333
Kzo9I GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
LguI GCTCTTC 1 cut(s) 1363
LmnI GCTCC 3 cut(s) 703, 1006, 1392
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 4 cut(s) 446, 811, 1116, 1179
MaeI CTAG 6 cut(s) 192, 204, 417, 564, 707, 1029
MaeIII GTNAC 5 cut(s) 92, 377, 617, 868, 1059
MalI GATC 6 cut(s) 278, 444, 745, 1037, 1230, 1335
MboI GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
MboII GAAGA 7 cut(s) 505, 508, 527, 610, 797, 845, 1350
MhlI GDGCHC 3 cut(s) 642, 981, 1397
MluCI AATT 5 cut(s) 39, 306, 1177, 1291, 1424
MlyI GAGTC 2 cut(s) 830, 1067
MmeI TCCRAC 3 cut(s) 90, 649, 1308
MroXI GAANNNNTTC 2 cut(s) 354, 589
MseI TTAA 8 cut(s) 254, 309, 480, 579, 1137, 1286, 1422, 1441
MslI CAYNNNNRTG 1 cut(s) 698
MspCI CTTAAG 1 cut(s) 1285
MspI CCGG 1 cut(s) 546
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 1023
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 568
NdeII GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
NlaIV GGNNCC 7 cut(s) 17, 52, 171, 633, 966, 1046, 1241
NmuCI GTSAC 4 cut(s) 92, 377, 617, 1059
NspI RCATGY 1 cut(s) 439
PaeI GCATGC 1 cut(s) 439
PagI TCATGA 1 cut(s) 1336
PciSI GCTCTTC 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 1 cut(s) 1212
PctI GAATGC 1 cut(s) 1023
PdmI GAANNNNTTC 2 cut(s) 354, 589
PfeI GAWTC 4 cut(s) 285, 527, 590, 1322
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 2 cut(s) 829, 1066
PpsI GAGTC 2 cut(s) 829, 1066
PpuMI RGGWCCY 2 cut(s) 169, 965
PshBI ATTAAT 1 cut(s) 579
Psp124BI GAGCTC 1 cut(s) 1397
Psp5II RGGWCCY 2 cut(s) 169, 965
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 7 cut(s) 17, 52, 171, 633, 966, 1046, 1241
PspPI GGNCC 3 cut(s) 50, 169, 965
PspPPI RGGWCCY 2 cut(s) 169, 965
PstI CTGCAG 1 cut(s) 877
RsaI GTAC 4 cut(s) 17, 208, 362, 731
RsaNI GTAC 4 cut(s) 16, 207, 361, 730
RseI CAYNNNNRTG 1 cut(s) 698
SacI GAGCTC 1 cut(s) 1397
SapI GCTCTTC 1 cut(s) 1363
SaqAI TTAA 8 cut(s) 254, 309, 480, 579, 1137, 1286, 1422, 1441
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 6 cut(s) 276, 442, 743, 1035, 1228, 1333
Sau96I GGNCC 3 cut(s) 50, 169, 965
SchI GAGTC 2 cut(s) 830, 1067
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 3 cut(s) 642, 981, 1397
SfaNI GCATC 4 cut(s) 446, 811, 1116, 1179
SfcI CTRYAG 1 cut(s) 873
SinI GGWCC 3 cut(s) 50, 169, 965
SmiMI CAYNNNNRTG 1 cut(s) 698
SmlI CTYRAG 3 cut(s) 1153, 1285, 1396
SmoI CTYRAG 3 cut(s) 1153, 1285, 1396
SpeI ACTAGT 2 cut(s) 191, 203
SphI GCATGC 1 cut(s) 439
Sse9I AATT 5 cut(s) 39, 306, 1177, 1291, 1424
SspI AATATT 1 cut(s) 1195
SspMI CTAG 6 cut(s) 192, 204, 417, 564, 707, 1029
SstI GAGCTC 1 cut(s) 1397
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 2 cut(s) 563, 774
TaaI ACNGT 2 cut(s) 236, 617
TaqI TCGA 1 cut(s) 1206
TasI AATT 5 cut(s) 39, 306, 1177, 1291, 1424
TfiI GAWTC 4 cut(s) 285, 527, 590, 1322
Tru1I TTAA 8 cut(s) 254, 309, 480, 579, 1137, 1286, 1422, 1441
Tru9I TTAA 8 cut(s) 254, 309, 480, 579, 1137, 1286, 1422, 1441
TscAI CASTG 2 cut(s) 295, 732
TseFI GTSAC 4 cut(s) 92, 377, 617, 1059
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 4 cut(s) 92, 377, 617, 1059
TspDTI ATGAA 3 cut(s) 582, 894, 1134
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 2 cut(s) 295, 732
Vha464I CTTAAG 1 cut(s) 1285
VpaK11BI GGWCC 3 cut(s) 50, 169, 965
VspI ATTAAT 1 cut(s) 579
XagI CCTNNNNNAGG 2 cut(s) 178, 253
XapI RAATTY 1 cut(s) 1291
XceI RCATGY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 583
XmaJI CCTAGG 1 cut(s) 563
XmnI GAANNNNTTC 2 cut(s) 354, 589
XspI CTAG 6 cut(s) 192, 204, 417, 564, 707, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.