MD14G1207200.v1.1

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011359
Physical Location & Seq
Reverse (-)
29416282 .. 29418975
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1207200.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGGCTTCTGAAACTTACCAACCCAAAAATGTATGTGTGATTGGAGCTGGACCCTCGGGGCTTGTAGCTGCAAGGGAGCTGAGGAAAGAAGGTCACAGGGTGGTGGTGTTGGAACAAAACCATGAGGTGGGAGGGCAGTGGCTCTACGACCCAAATGTGGAGGGAGAGGATCCCTTAGGAAGGTCACCTACCCTAAAAGTGCATAGCAGTCTTTACTCCTCTTTGAGGATAATTTCTCCAAGAGAGAGCATGGGGTTCACTGACTTCCCATTTGCAGTCAAGAAAGGTAGGGACATGAGGAGGTTTCCTGGCCATACGGAGCTTCTTTTGTACCTCAAGGATTTTTGTGATTGGTTTGGATTGAGGGAGTTGATAAGGTTCAATACGAGGGTTTCATATGTGGGGATGTTGGATGGAGATCATGTAGTTGGTTGCAAAGATCTGAAATGGGTTGTGAGGAGTATGGAGAAGAAGGCTGAGATTTTCGTGGAGGAGGTGTTTGATGCGGTGGTTGTGGCCTCCGGCCATTACTCTAACCCAAGGTTACCGTCCATTAAAGGAATGGATGCATGGAAGAGGAAGCAAATGCACAGTCACATCTATAGGGTTCCGGAGCCATTTCGTGATGAGGTTGTGGTGGTGGTTGCGAATTCACTAAGTGGACAAGATATATCAATAGACCTAGTGGCTGTGGCCAAGGCAATCTATCTTAGTGCCAGATCGCTTGACATATCTAAGGGCTTGTCCAAAGTCATTTCCAAACACGAAAACTTGCACCTTCGTCCCCAGATAGAGTCGCTTCAAGAAGATGGGAAAGTATTATTTGTGGACGGTTCTTGGATCACTGCAGACACTATCATATATTGTACTGGGTATTCATACACGTTCTCCTTTCTTGACACCAAAGGAATAGTAACTGTGGATGATAACAGAGTGGGCCCCTTGTATGAGCACACCTTCCCTCCATCACTTGCTCCCTCTTTATCTTTTGTAGGCATTCCAAAAAAGGTCATAGGGTTCCTGTTCTTTGAGTCACAAGCAAAATGGATAGCTCAACTGCTCTCTGGGAAAACAACACTGCCATCAAGGGATGACATGATGCAGTCCATCAAAGAGTTCTACCACTCAAGGGAGGTTGCTGGCATTCCAAAACATGACACCCACGGCATCGGCGACTTCGAGTATTGTGATAGATATGGAGATCACTCGGGGTTCCCACATTTGGAAGAATGGAGGAAAGAGCTTTGCTATTCAGCACAAATACATGCCGACGCCGACTTAACATTCAGGGATTCGTGCGATGATCATGAGCTGCTTCAAAAGGCTTTTCAAAGTCCATATTTTGCTCAATTTCGGCCTCAAGATTTAGATTTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like K_oxygenase

Protein Analysis

460

Amino Acids

52.08

Weight (kDa)

6.06

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 5 - 47 2.8e-06 Thi4 family
FMO-like PF00743 9 - 241 5.9e-30 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 10 - 306 2.4e-14 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 10 - 44 3.8e-06 HI0933-like protein Rossmann domain
FAD_binding_3 PF01494 10 - 41 4e-06 FAD binding domain
DAO PF01266 11 - 45 3.4e-07 FAD dependent oxidoreductase
FAD_oxidored PF12831 11 - 47 5.2e-06 FAD dependent oxidoreductase
FAD_binding_2 PF00890 11 - 46 8.1e-06 FAD binding domain
NAD_binding_8 PF13450 13 - 47 2.5e-10 NAD(P)-binding Rossmann-like domain
Pyr_redox_3 PF13738 76 - 312 1.7e-12 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 258 - 373 2.6e-15 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07800
fragaria_vesca FvH4_5g14390
malus_domestica MD06G1199100.v1.1 MD14G1206500.v1.1 MD14G1206800.v1.1 MD14G1207200.v1.1
prunus_persica Prupe.5G199900_v2.0.a1
pyrus_communis pycom14g17100 pycom14g17110 pycom14g17120
rosa_chinensis RchiOBHm_Chr7g0182301
rosa_laevigata RLG00000005134
rosa_multiflora Rmu_sc0000003.1_g000007
rosa_roxburghii Rroxscaffold_3G00271340
rosa_rugosa Rorug06G0446900
rosa_samantha Rh7AG049800 Rh7BG049900 Rh7CG051200 Rh7DG049100
rosa_wichuraiana Rw7G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 127
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 1 cut(s) 506
AclWI GGATC 3 cut(s) 164, 177, 848
AcoI YGGCCR 3 cut(s) 310, 523, 693
AcsI RAATTY 1 cut(s) 649
AcyI GRCGYC 1 cut(s) 1272
AdeI CACNNNGTG 2 cut(s) 100, 659
AfaI GTAC 2 cut(s) 332, 868
AfiI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
AflIII ACRYGT 1 cut(s) 882
AgsI TTSAA 4 cut(s) 382, 803, 1319, 1331
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 7 cut(s) 47, 68, 79, 322, 1052, 1243, 1312
AluI AGCT 7 cut(s) 47, 68, 79, 322, 1052, 1243, 1312
Alw21I GWGCWC 1 cut(s) 954
AlwI GGATC 3 cut(s) 164, 177, 848
Ama87I CYCGRG 2 cut(s) 55, 1207
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 6 cut(s) 310, 516, 523, 693, 937, 1355
ApaI GGGCCC 1 cut(s) 941
ApeKI GCWGC 2 cut(s) 68, 1312
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 577, 609
AspS9I GGNCC 3 cut(s) 50, 937, 938
AsuHPI GGTGA 1 cut(s) 177
AvaI CYCGRG 2 cut(s) 55, 1207
AvaII GGWCC 1 cut(s) 50
AxyI CCTNAGG 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 941
BalI TGGCCA 2 cut(s) 312, 695
BamHI GGATCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 941
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
Bbv12I GWGCWC 1 cut(s) 954
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 55, 1299
BccI CCATC 5 cut(s) 407, 803, 973, 1090, 1115
BceAI ACGGC 1 cut(s) 1180
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 1 cut(s) 1303
BfaI CTAG 1 cut(s) 683
BfmI CTRYAG 2 cut(s) 601, 846
BglII AGATCT 1 cut(s) 439
BisI GCNGC 2 cut(s) 69, 1313
BlsI GCNGC 2 cut(s) 70, 1314
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 50
BmeT110I CYCGRG 2 cut(s) 55, 1207
BmgT120I GGNCC 3 cut(s) 50, 937, 938
BmiI GGNNCC 8 cut(s) 52, 171, 609, 615, 939, 940, 1019, 1214
BmrFI CCNGG 1 cut(s) 309
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 4 cut(s) 493, 556, 1089, 1176
BmuI ACTGGG 1 cut(s) 879
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 3 cut(s) 320, 1111, 1344
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 1272
BsaJI CCNNGG 4 cut(s) 54, 539, 696, 1162
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 8 cut(s) 408, 438, 482, 512, 872, 902, 946, 976
Bsc4I CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
Bse1I ACTGG 1 cut(s) 874
Bse21I CCTNAGG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 54, 539, 696, 1162
BseGI GGATG 5 cut(s) 411, 418, 571, 928, 1096
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
BseMII CTCAG 2 cut(s) 71, 468
BseNI ACTGG 1 cut(s) 874
BseRI GAGGAG 4 cut(s) 208, 313, 472, 506
BseSI GKGCMC 1 cut(s) 941
BseXI GCAGC 2 cut(s) 55, 1299
BshFI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1357
BsiHKAI GWGCWC 1 cut(s) 954
BsiHKCI CYCGRG 2 cut(s) 55, 1207
BsiSI CCGG 2 cut(s) 522, 611
BslFI GGGAC 2 cut(s) 305, 768
BslI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 225, 1129, 1222
BsmFI GGGAC 2 cut(s) 305, 768
BsmI GAATGC 2 cut(s) 996, 1143
BsnI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1357
BsoBI CYCGRG 2 cut(s) 55, 1207
Bsp120I GGGCCC 1 cut(s) 937
Bsp1286I GDGCHC 2 cut(s) 941, 954
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
BspACI CCGC 1 cut(s) 506
BspANI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1357
BspCNI CTCAG 2 cut(s) 72, 469
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 1306
BspLI GGNNCC 8 cut(s) 52, 171, 609, 615, 939, 940, 1019, 1214
BspMAI CTGCAG 1 cut(s) 850
BspPI GGATC 3 cut(s) 164, 177, 848
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 4 cut(s) 54, 539, 696, 1162
BssMI GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
BssNI GRCGYC 1 cut(s) 1272
BssT1I CCWWGG 2 cut(s) 539, 696
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 4 cut(s) 549, 593, 833, 919
Bst6I CTCTTC 1 cut(s) 569
BstACI GRCGYC 1 cut(s) 1272
BstC8I GCNNGC 1 cut(s) 1141
BstDEI CTNAG 6 cut(s) 80, 175, 477, 656, 710, 735
BstDSI CCRYGG 1 cut(s) 1162
BstEII GGTNACC 2 cut(s) 183, 543
BstENI CCTNNNNNAGG 2 cut(s) 178, 223
BstF5I GGATG 5 cut(s) 411, 418, 571, 928, 1096
BstKTI GATC 7 cut(s) 172, 421, 442, 722, 843, 1204, 1306
BstMBI GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
BstNI CCWGG 1 cut(s) 309
BstNSI RCATGY 1 cut(s) 1268
BstPI GGTNACC 2 cut(s) 183, 543
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 2 cut(s) 601, 846
BstSLI GKGCMC 1 cut(s) 941
BstV1I GCAGC 2 cut(s) 55, 1299
BstX2I RGATCY 2 cut(s) 169, 439
BstYI RGATCY 2 cut(s) 169, 439
Bsu36I CCTNAGG 1 cut(s) 175
BsuRI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1357
BtgI CCRYGG 1 cut(s) 1162
BtgZI GCGATG 1 cut(s) 1314
BtsCI GGATG 5 cut(s) 411, 418, 571, 928, 1096
BtsI GCAGTG 3 cut(s) 143, 843, 1076
BtsIMutI CAGTG 4 cut(s) 143, 258, 843, 1076
Cac8I GCNNGC 1 cut(s) 1141
CciI TCATGA 1 cut(s) 1306
Cfr13I GGNCC 3 cut(s) 50, 937, 938
CseI GACGC 1 cut(s) 1280
Csp6I GTAC 2 cut(s) 331, 867
CviAII CATG 9 cut(s) 122, 250, 295, 422, 570, 1096, 1154, 1265, 1307
CviQI GTAC 2 cut(s) 331, 867
DdeI CTNAG 6 cut(s) 80, 175, 477, 656, 710, 735
DpnI GATC 7 cut(s) 171, 420, 441, 721, 842, 1203, 1305
DpnII GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
DraIII CACNNNGTG 2 cut(s) 100, 659
EaeI YGGCCR 3 cut(s) 310, 523, 693
Eam1104I CTCTTC 1 cut(s) 569
EarI CTCTTC 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 539, 696
Eco24I GRGCYC 1 cut(s) 941
Eco47I GGWCC 1 cut(s) 50
Eco81I CCTNAGG 1 cut(s) 175
Eco88I CYCGRG 2 cut(s) 55, 1207
Eco91I GGTNACC 2 cut(s) 183, 543
EcoNI CCTNNNNNAGG 2 cut(s) 178, 223
EcoO109I RGGNCCY 1 cut(s) 938
EcoO65I GGTNACC 2 cut(s) 183, 543
EcoRI GAATTC 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 539, 696
EcoT22I ATGCAT 1 cut(s) 571
EcoT38I GRGCYC 1 cut(s) 941
ErhI CCWWGG 2 cut(s) 539, 696
FaeI CATG 9 cut(s) 125, 253, 298, 425, 573, 1099, 1157, 1268, 1310
FaqI GGGAC 2 cut(s) 305, 768
FatI CATG 9 cut(s) 121, 249, 294, 421, 569, 1095, 1153, 1264, 1306
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 1303
Fnu4HI GCNGC 2 cut(s) 69, 1313
FokI GGATG 5 cut(s) 418, 425, 578, 935, 1103
FriOI GRGCYC 1 cut(s) 941
Fsp4HI GCNGC 2 cut(s) 69, 1313
FspBI CTAG 1 cut(s) 683
GluI GCNGC 2 cut(s) 69, 1313
HaeIII GGCC 6 cut(s) 312, 518, 525, 695, 939, 1357
HapII CCGG 2 cut(s) 522, 611
HgaI GACGC 1 cut(s) 1280
Hin1I GRCGYC 1 cut(s) 1272
Hin1II CATG 9 cut(s) 125, 253, 298, 425, 573, 1099, 1157, 1268, 1310
HinfI GANTC 3 cut(s) 794, 1031, 1292
HpaII CCGG 2 cut(s) 522, 611
HphI GGTGA 1 cut(s) 177
Hpy166II GTNNAC 3 cut(s) 258, 662, 829
Hpy188I TCNGA 3 cut(s) 10, 444, 1379
Hpy188III TCNNGA 7 cut(s) 280, 611, 623, 803, 896, 1307, 1361
Hpy8I GTNNAC 3 cut(s) 258, 662, 829
Hpy99I CGWCG 1 cut(s) 1274
HpyAV CCTTC 5 cut(s) 83, 174, 466, 788, 967
HpyCH4III ACNGT 4 cut(s) 549, 593, 833, 919
HpyCH4IV ACGT 1 cut(s) 884
HpyCH4V TGCA 9 cut(s) 71, 202, 275, 435, 569, 589, 775, 848, 1102
HpyF3I CTNAG 6 cut(s) 80, 175, 477, 656, 710, 735
HpySE526I ACGT 1 cut(s) 884
Hsp92I GRCGYC 1 cut(s) 1272
Hsp92II CATG 9 cut(s) 125, 253, 298, 425, 573, 1099, 1157, 1268, 1310
Kpn2I TCCGGA 1 cut(s) 610
Ksp22I TGATCA 1 cut(s) 1303
Kzo9I GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
LmnI GCTCC 5 cut(s) 44, 76, 319, 613, 979
Lsp1109I GCAGC 2 cut(s) 55, 1299
LweI GCATC 4 cut(s) 493, 556, 1089, 1176
MaeI CTAG 1 cut(s) 683
MaeII ACGT 1 cut(s) 884
MaeIII GTNAC 6 cut(s) 92, 183, 543, 593, 913, 1032
MalI GATC 7 cut(s) 171, 420, 441, 721, 842, 1203, 1305
MboI GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
MboII GAAGA 4 cut(s) 481, 586, 818, 1238
MflI RGATCY 2 cut(s) 169, 439
MhlI GDGCHC 2 cut(s) 941, 954
MlsI TGGCCA 2 cut(s) 312, 695
MluCI AATT 3 cut(s) 231, 649, 1349
MluNI TGGCCA 2 cut(s) 312, 695
MlyI GAGTC 2 cut(s) 803, 1040
MmeI TCCRAC 2 cut(s) 90, 390
Mox20I TGGCCA 2 cut(s) 312, 695
Mph1103I ATGCAT 1 cut(s) 571
MroI TCCGGA 1 cut(s) 610
MscI TGGCCA 2 cut(s) 312, 695
MseI TTAA 2 cut(s) 555, 1280
Msp20I TGGCCA 2 cut(s) 312, 695
MspI CCGG 2 cut(s) 522, 611
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 996, 1143
MvaI CCWGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 397
NdeII GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
NlaIII CATG 9 cut(s) 125, 253, 298, 425, 573, 1099, 1157, 1268, 1310
NlaIV GGNNCC 8 cut(s) 52, 171, 609, 615, 939, 940, 1019, 1214
NmuCI GTSAC 4 cut(s) 92, 183, 593, 1032
NsiI ATGCAT 1 cut(s) 571
NspI RCATGY 1 cut(s) 1268
PagI TCATGA 1 cut(s) 1306
PcsI WCGNNNNNNNCGW 2 cut(s) 1170, 1176
PctI GAATGC 2 cut(s) 996, 1143
PfeI GAWTC 1 cut(s) 1292
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 2 cut(s) 70, 1314
PleI GAGTC 2 cut(s) 802, 1039
PpsI GAGTC 2 cut(s) 802, 1039
Psp6I CCWGG 1 cut(s) 307
PspEI GGTNACC 2 cut(s) 183, 543
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 8 cut(s) 52, 171, 609, 615, 939, 940, 1019, 1214
PspOMI GGGCCC 1 cut(s) 937
PspPI GGNCC 3 cut(s) 50, 937, 938
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 2 cut(s) 169, 439
RsaI GTAC 2 cut(s) 332, 868
RsaNI GTAC 2 cut(s) 331, 867
SaqAI TTAA 2 cut(s) 555, 1280
SatI GCNGC 2 cut(s) 69, 1313
Sau3AI GATC 7 cut(s) 169, 418, 439, 719, 840, 1201, 1303
Sau96I GGNCC 3 cut(s) 50, 937, 938
SchI GAGTC 2 cut(s) 803, 1040
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 2 cut(s) 941, 954
SfaNI GCATC 4 cut(s) 493, 556, 1089, 1176
SfcI CTRYAG 2 cut(s) 601, 846
SinI GGWCC 1 cut(s) 50
SmlI CTYRAG 3 cut(s) 335, 1126, 1359
SmoI CTYRAG 3 cut(s) 335, 1126, 1359
Sse9I AATT 3 cut(s) 231, 649, 1349
SsiI CCGC 1 cut(s) 506
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 2 cut(s) 539, 696
TaaI ACNGT 4 cut(s) 549, 593, 833, 919
TaiI ACGT 1 cut(s) 887
TaqI TCGA 1 cut(s) 1179
TasI AATT 3 cut(s) 231, 649, 1349
TatI WGTACW 1 cut(s) 866
TfiI GAWTC 1 cut(s) 1292
Tru1I TTAA 2 cut(s) 555, 1280
Tru9I TTAA 2 cut(s) 555, 1280
TscAI CASTG 4 cut(s) 143, 265, 850, 1083
TseFI GTSAC 4 cut(s) 92, 183, 593, 1032
TseI GCWGC 2 cut(s) 68, 1312
Tsp45I GTSAC 4 cut(s) 92, 183, 593, 1032
TspDTI ATGAA 2 cut(s) 384, 867
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 4 cut(s) 143, 265, 850, 1083
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 50
XagI CCTNNNNNAGG 2 cut(s) 178, 223
XapI RAATTY 1 cut(s) 649
XceI RCATGY 1 cut(s) 1268
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XspI CTAG 1 cut(s) 683
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.