FvH4_5g15180

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013934
Physical Location & Seq
Forward (+)
8572654 .. 8574548
1895 bp
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UTR
Exon/CDS
Intron
FvH4_5g15180.t1

Sequence Viewer

Length: 522 bp
ATGGGATCTGGTGCCGGAAGTTTCCTCAAAGTTCTCCTTAAAAACTTCGATGTTCTTGCCGGGCCTGTGGTTAGTTTGGTTTACCCTCTGTACGCCTCAATTAGGGCTATAGAGACCAAATCTGCAACTGATGACCGCCAATGGCTTACTTATTGGATTCTCTATTCTATGATTACACTCTTTGAGCTCACCTTTGCCAAACTTATTGAGTGGATCCCAATCTGGTCATATGCCAAGCTGATCATGACTTGCTGGTTGGTCATCCCATACTTCAGTGGTGCTGCATATGTCTATGAACACTATGTCAGGCCGTTTTTCCTCAACCCACAACAGACGATCAATATTTGGTATGTTCCAAGGAAGAAGGATGGCTTTAGTAAGCCAGATGATGTTCTAACAGCTGCAGAGAAGTACATTGAAGAGAATGGTACTGAAGCATTCGAGAAACTCATTCATAGGTCTGACAAGTCCCAAAGGAGTGGAGGATCCATCTTTGATGATGATCATAGGTACTACTACTGA

Protein Analysis

174

Amino Acids

20.16

Weight (kDa)

6.29

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 29 - 105 3.5e-28 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013934)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74520 AT1G74520
fragaria_vesca FvH4_5g15180
malus_domestica MD00G1186100.v1.1 MD06G1191500.v1.1 MD14G1197900.v1.1
prunus_persica Prupe.5G190000_v2.0.a1 Prupe.5G190000_v2.0.a1
pyrus_communis pycom06g16960
rosa_chinensis RchiOBHm_Chr7g0180981
rosa_multiflora Rmu_sc0004056.1_g000003
rosa_roxburghii Rroxscaffold_3G00272360
rosa_rugosa Rorug06G0438100 Rorug06G0438200
rosa_samantha Rh7CG040700 Rh7DG039100
rosa_wichuraiana Rw7G003250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AciI CCGC 1 cut(s) 136
AclWI GGATC 5 cut(s) 13, 208, 221, 480, 493
AcuI CTGAAG 2 cut(s) 256, 453
AfaI GTAC 4 cut(s) 92, 413, 430, 512
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 1 cut(s) 419
AluBI AGCT 3 cut(s) 187, 238, 401
AluI AGCT 3 cut(s) 187, 238, 401
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 5 cut(s) 13, 208, 221, 480, 493
AoxI GGCC 2 cut(s) 62, 308
ApeKI GCWGC 2 cut(s) 281, 401
AspS9I GGNCC 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 181
BamHI GGATCC 2 cut(s) 213, 485
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 268, 388
BccI CCATC 2 cut(s) 362, 497
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BclI TGATCA 2 cut(s) 240, 502
BcnI CCSGG 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 2 cut(s) 108, 402
BisI GCNGC 2 cut(s) 282, 402
BlsI GCNGC 2 cut(s) 283, 403
Bme1390I CCNGG 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 3 cut(s) 13, 215, 487
BmrFI CCNGG 1 cut(s) 61
BpuMI CCSGG 1 cut(s) 61
BsaBI GATNNNNATC 2 cut(s) 218, 501
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 356
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse8I GATNNNNATC 2 cut(s) 218, 501
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 261, 373
BseJI GATNNNNATC 2 cut(s) 218, 501
BseLI CCNNNNNNNGG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 268, 388
BshFI GGCC 2 cut(s) 64, 310
BshNI GGYRCC 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 2 cut(s) 15, 60
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 2 cut(s) 64, 310
Bso31I GGTCTC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 6 cut(s) 5, 213, 240, 336, 485, 502
BspACI CCGC 1 cut(s) 136
BspANI GGCC 2 cut(s) 64, 310
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 3 cut(s) 13, 215, 487
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 5 cut(s) 13, 208, 221, 480, 493
BspT107I GGYRCC 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 6 cut(s) 5, 213, 240, 336, 485, 502
BssT1I CCWWGG 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 414
BstENI CCTNNNNNAGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 261, 373
BstKTI GATC 6 cut(s) 8, 216, 243, 339, 488, 505
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 6 cut(s) 5, 213, 240, 336, 485, 502
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 2 cut(s) 108, 402
BstV1I GCAGC 2 cut(s) 268, 388
BstX2I RGATCY 3 cut(s) 5, 213, 485
BstXI CCANNNNNNTGG 1 cut(s) 479
BstYI RGATCY 3 cut(s) 5, 213, 485
BsuRI GGCC 2 cut(s) 64, 310
BtsCI GGATG 2 cut(s) 261, 373
BtsIMutI CAGTG 1 cut(s) 280
CciI TCATGA 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 62
Csp6I GTAC 4 cut(s) 91, 412, 429, 511
CviAII CATG 1 cut(s) 244
CviJI RGCY 9 cut(s) 64, 107, 145, 187, 238, 310, 372, 382, 401
CviKI_1 RGCY 9 cut(s) 64, 107, 145, 187, 238, 310, 372, 382, 401
CviQI GTAC 4 cut(s) 91, 412, 429, 511
DpnI GATC 6 cut(s) 7, 215, 242, 338, 487, 504
DpnII GATC 6 cut(s) 5, 213, 240, 336, 485, 502
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 356
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 107
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 2 cut(s) 256, 453
EcoICRI GAGCTC 1 cut(s) 187
EcoNI CCTNNNNNAGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 1 cut(s) 247
FalI AAGNNNNNCTT 4 cut(s) 21, 53, 356, 388
FaqI GGGAC 1 cut(s) 454
FatI CATG 1 cut(s) 243
FauNDI CATATG 2 cut(s) 229, 286
FbaI TGATCA 2 cut(s) 240, 502
Fnu4HI GCNGC 2 cut(s) 282, 402
FokI GGATG 2 cut(s) 248, 380
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 282, 402
GluI GCNGC 2 cut(s) 282, 402
HaeIII GGCC 2 cut(s) 64, 310
HapII CCGG 2 cut(s) 15, 60
Hin1II CATG 1 cut(s) 247
HinfI GANTC 1 cut(s) 157
HpaII CCGG 2 cut(s) 15, 60
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 2 cut(s) 244, 442
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 358
HpyCH4V TGCA 3 cut(s) 125, 284, 404
Hsp92II CATG 1 cut(s) 247
Ksp22I TGATCA 2 cut(s) 240, 502
Kzo9I GATC 6 cut(s) 5, 213, 240, 336, 485, 502
LpnPI CCDG 7 cut(s) 28, 73, 78, 208, 238, 292, 396
Lsp1109I GCAGC 2 cut(s) 268, 388
MalI GATC 6 cut(s) 7, 215, 242, 338, 487, 504
MboI GATC 6 cut(s) 5, 213, 240, 336, 485, 502
MboII GAAGA 2 cut(s) 373, 431
MflI RGATCY 3 cut(s) 5, 213, 485
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 1 cut(s) 99
MnlI CCTC 5 cut(s) 35, 96, 106, 329, 476
MseI TTAA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 2 cut(s) 15, 60
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 1 cut(s) 437
NciI CCSGG 1 cut(s) 61
NdeI CATATG 2 cut(s) 229, 286
NdeII GATC 6 cut(s) 5, 213, 240, 336, 485, 502
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 3 cut(s) 13, 215, 487
PagI TCATGA 1 cut(s) 243
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 1 cut(s) 157
PkrI GCNGC 2 cut(s) 283, 403
Psp124BI GAGCTC 1 cut(s) 189
PspN4I GGNNCC 3 cut(s) 13, 215, 487
PspPI GGNCC 1 cut(s) 62
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 3 cut(s) 5, 213, 485
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 4 cut(s) 92, 413, 430, 512
RsaNI GTAC 4 cut(s) 91, 412, 429, 511
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 2 cut(s) 282, 402
Sau3AI GATC 6 cut(s) 5, 213, 240, 336, 485, 502
Sau96I GGNCC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 1 cut(s) 189
SetI ASST 6 cut(s) 189, 194, 240, 403, 461, 512
SfcI CTRYAG 2 cut(s) 108, 402
Sse9I AATT 1 cut(s) 99
SsiI CCGC 1 cut(s) 136
SspI AATATT 1 cut(s) 343
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 1 cut(s) 356
TaqI TCGA 2 cut(s) 48, 441
TasI AATT 1 cut(s) 99
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 1 cut(s) 280
TseI GCWGC 2 cut(s) 281, 401
TspDTI ATGAA 2 cut(s) 309, 443
TspRI CASTG 1 cut(s) 280
XagI CCTNNNNNAGG 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.