MD14G1197900.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013934
Physical Location & Seq
Reverse (-)
28803707 .. 28805725
2019 bp
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UTR
Exon/CDS
Intron
MD14G1197900.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGGATCTGGCGCCGGCAGCTTCCTCAAAGTGCTTCTCAAGAACTTCGACGTTCTTGCTGGGCCTGTGTTTAGCCTTGTTTATCCACTATACGCATCGATTCGAGCGATAGAGACGCAGTCTACGACCGACGATCGCCAATGGCTGACATATTGGATTCTGTATTCCATGATTACTCTGATTGAACTCACCTTTGCCAAAGTCATTGAGTGGATTCCAATCTGGTCGTACGCTAAGCTGATTGCGACTTGTTGGTTGGTACTCCCGTACACGAATGGTGCTGCATACGTTTATGAACATTATATCCGACCTTTCTTTCTCAACCCGCATAAAATTAACATTTGGTATGTTCCGAGGAAGAAGGATGACCCAGATGACATTTTAGTTGCTGCGGAGAAATACATTGCAGAGAACGGGACTGAAGCTTTCGAGAAGATCATCCACAGGGCTGATAACAACCAGAGGAGCGGGGGATCCGTCTATCATAGGGGATCTGTCTTTGATGAGGACCTTAAGTACTATCGAGAATATTAG

Protein Analysis

178

Amino Acids

20.61

Weight (kDa)

6.07

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 29 - 105 3.9e-29 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013934)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74520 AT1G74520
fragaria_vesca FvH4_5g15180
malus_domestica MD00G1186100.v1.1 MD06G1191500.v1.1 MD14G1197900.v1.1
prunus_persica Prupe.5G190000_v2.0.a1 Prupe.5G190000_v2.0.a1
pyrus_communis pycom06g16960
rosa_chinensis RchiOBHm_Chr7g0180981
rosa_multiflora Rmu_sc0004056.1_g000003
rosa_roxburghii Rroxscaffold_3G00272360
rosa_rugosa Rorug06G0438100 Rorug06G0438200
rosa_samantha Rh7CG040700 Rh7DG039100
rosa_wichuraiana Rw7G003250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AccBSI CCGCTC 1 cut(s) 468
AccI GTMKAC 1 cut(s) 122
AciI CCGC 3 cut(s) 326, 392, 468
AclWI GGATC 4 cut(s) 13, 468, 481, 499
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 4 cut(s) 230, 261, 269, 518
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 1 cut(s) 185
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 3 cut(s) 21, 238, 425
AluI AGCT 3 cut(s) 21, 238, 425
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 4 cut(s) 13, 468, 481, 499
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 3 cut(s) 18, 281, 389
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 2 cut(s) 62, 508
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 508
BaeI ACNNNNGTAYC 2 cut(s) 251, 284
BamHI GGATCC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 30, 268, 376
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BcoDI GTCTC 1 cut(s) 107
BfoI RGCGCY 1 cut(s) 15
BfrI CTTAAG 1 cut(s) 512
BisI GCNGC 3 cut(s) 19, 282, 390
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 3 cut(s) 20, 283, 391
BmcAI AGTACT 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 62, 508
BmiI GGNNCC 2 cut(s) 13, 475
BmsI GCATC 1 cut(s) 104
Bpu1102I GCTNAGC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 23
Bsa29I ATCGAT 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 353
Bse118I RCCGGY 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 218
BseCI ATCGAT 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 353
BseGI GGATG 2 cut(s) 370, 438
BseJI GATNNNNATC 1 cut(s) 218
BseMI GCAATG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 478
BseXI GCAGC 3 cut(s) 30, 268, 376
BseYI CCCAGC 1 cut(s) 59
Bsh1285I CGRYCG 2 cut(s) 129, 136
BshFI GGCC 1 cut(s) 64
BshNI GGYRCC 1 cut(s) 11
BshVI ATCGAT 1 cut(s) 98
BsiEI CGRYCG 2 cut(s) 129, 136
BsiSI CCGG 1 cut(s) 15
BsiWI CGTACG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 430
BsnI GGCC 1 cut(s) 64
Bsp143I GATC 5 cut(s) 5, 133, 435, 473, 491
Bsp1720I GCTNAGC 1 cut(s) 234
BspACI CCGC 3 cut(s) 326, 392, 468
BspANI GGCC 1 cut(s) 64
BspDI ATCGAT 1 cut(s) 98
BspLI GGNNCC 2 cut(s) 13, 475
BspPI GGATC 4 cut(s) 13, 468, 481, 499
BspT107I GGYRCC 1 cut(s) 11
BspTI CTTAAG 1 cut(s) 512
BsrBI CCGCTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 402
BsrFI RCCGGY 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 353
BssMI GATC 5 cut(s) 5, 133, 435, 473, 491
BssNI GRCGYC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 12
BstAFI CTTAAG 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 234
BstF5I GGATG 2 cut(s) 370, 438
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 5 cut(s) 8, 136, 438, 476, 494
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 5 cut(s) 5, 133, 435, 473, 491
BstMCI CGRYCG 2 cut(s) 129, 136
BstMWI GCNNNNNNNGC 1 cut(s) 18
BstV1I GCAGC 3 cut(s) 30, 268, 376
BstX2I RGATCY 3 cut(s) 5, 473, 491
BstYI RGATCY 3 cut(s) 5, 473, 491
Bsu15I ATCGAT 1 cut(s) 98
BsuRI GGCC 1 cut(s) 64
BsuTUI ATCGAT 1 cut(s) 98
BtsCI GGATG 2 cut(s) 370, 438
Cac8I GCNNGC 1 cut(s) 16
CfoI GCGC 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 62, 508
ClaI ATCGAT 1 cut(s) 98
CseI GACGC 1 cut(s) 124
Csp6I GTAC 4 cut(s) 229, 260, 268, 517
CviAII CATG 1 cut(s) 169
CviJI RGCY 7 cut(s) 21, 64, 75, 145, 238, 425, 449
CviKI_1 RGCY 7 cut(s) 21, 64, 75, 145, 238, 425, 449
CviQI GTAC 4 cut(s) 229, 260, 268, 517
DdeI CTNAG 1 cut(s) 234
DinI GGCGCC 1 cut(s) 13
DpnI GATC 5 cut(s) 7, 135, 437, 475, 493
DpnII GATC 5 cut(s) 5, 133, 435, 473, 491
Eco47I GGWCC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 508
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 1 cut(s) 172
FaiI YATR 9 cut(s) 91, 151, 170, 286, 294, 303, 330, 348, 486
FaqI GGGAC 1 cut(s) 430
FatI CATG 1 cut(s) 168
FauI CCCGC 2 cut(s) 333, 461
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 3 cut(s) 19, 282, 390
FokI GGATG 2 cut(s) 377, 425
Fsp4HI GCNGC 3 cut(s) 19, 282, 390
GlaI GCGC 1 cut(s) 13
GluI GCNGC 3 cut(s) 19, 282, 390
GsaI CCCAGC 1 cut(s) 63
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 15
HgaI GACGC 1 cut(s) 124
HhaI GCGC 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 1 cut(s) 172
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 3 cut(s) 100, 157, 214
HpaII CCGG 1 cut(s) 15
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 2 cut(s) 123, 270
Hpy188I TCNGA 3 cut(s) 180, 308, 354
Hpy188III TCNNGA 3 cut(s) 40, 430, 524
Hpy8I GTNNAC 2 cut(s) 123, 270
Hpy99I CGWCG 2 cut(s) 53, 134
HpyAV CCTTC 1 cut(s) 355
HpyCH4IV ACGT 2 cut(s) 51, 288
HpyCH4V TGCA 2 cut(s) 284, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 18
HpyF3I CTNAG 1 cut(s) 234
HpySE526I ACGT 2 cut(s) 51, 288
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 1 cut(s) 172
HspAI GCGC 1 cut(s) 12
KasI GGCGCC 1 cut(s) 11
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Kzo9I GATC 5 cut(s) 5, 133, 435, 473, 491
LmnI GCTCC 1 cut(s) 465
LpnPI CCDG 7 cut(s) 28, 45, 78, 208, 384, 430, 473
Lsp1109I GCAGC 3 cut(s) 30, 268, 376
LweI GCATC 1 cut(s) 104
MaeII ACGT 2 cut(s) 51, 288
MalI GATC 5 cut(s) 7, 135, 437, 475, 493
MbiI CCGCTC 1 cut(s) 468
MboI GATC 5 cut(s) 5, 133, 435, 473, 491
MboII GAAGA 2 cut(s) 370, 445
MflI RGATCY 3 cut(s) 5, 473, 491
MluCI AATT 1 cut(s) 333
Mly113I GGCGCC 1 cut(s) 12
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 4 cut(s) 35, 348, 456, 499
MroNI GCCGGC 1 cut(s) 14
MseI TTAA 2 cut(s) 336, 513
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 18
NaeI GCCGGC 1 cut(s) 16
NarI GGCGCC 1 cut(s) 12
NdeII GATC 5 cut(s) 5, 133, 435, 473, 491
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 2 cut(s) 13, 475
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 122
PdiI GCCGGC 1 cut(s) 16
PfeI GAWTC 3 cut(s) 100, 157, 214
Pfl23II CGTACG 1 cut(s) 228
PflFI GACNNNGTC 1 cut(s) 118
PkrI GCNGC 3 cut(s) 20, 283, 391
Ple19I CGATCG 1 cut(s) 136
PluTI GGCGCC 1 cut(s) 15
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
PspFI CCCAGC 1 cut(s) 59
PspLI CGTACG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 13, 475
PspPI GGNCC 2 cut(s) 62, 508
PspPPI RGGWCCY 1 cut(s) 508
PsuI RGATCY 3 cut(s) 5, 473, 491
PsyI GACNNNGTC 1 cut(s) 118
PvuI CGATCG 1 cut(s) 136
RsaI GTAC 4 cut(s) 230, 261, 269, 518
RsaNI GTAC 4 cut(s) 229, 260, 268, 517
SaqAI TTAA 2 cut(s) 336, 513
SatI GCNGC 3 cut(s) 19, 282, 390
Sau3AI GATC 5 cut(s) 5, 133, 435, 473, 491
Sau96I GGNCC 2 cut(s) 62, 508
ScaI AGTACT 1 cut(s) 518
SetI ASST 8 cut(s) 23, 54, 194, 240, 291, 313, 427, 513
SfaNI GCATC 1 cut(s) 104
SfoI GGCGCC 1 cut(s) 13
SinI GGWCC 1 cut(s) 508
SmlI CTYRAG 2 cut(s) 38, 512
SmoI CTYRAG 2 cut(s) 38, 512
Sse9I AATT 1 cut(s) 333
SsiI CCGC 3 cut(s) 326, 392, 468
SspDI GGCGCC 1 cut(s) 11
SspI AATATT 1 cut(s) 530
TaiI ACGT 2 cut(s) 54, 291
TaqI TCGA 5 cut(s) 48, 98, 103, 429, 523
TaqII GACCGA 1 cut(s) 143
TasI AATT 1 cut(s) 333
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 3 cut(s) 100, 157, 214
Tru1I TTAA 2 cut(s) 336, 513
Tru9I TTAA 2 cut(s) 336, 513
TseI GCWGC 3 cut(s) 18, 281, 389
TspDTI ATGAA 1 cut(s) 309
TspGWI ACGGA 1 cut(s) 466
Tth111I GACNNNGTC 1 cut(s) 118
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 122
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.