Rroxscaffold_3G00272360

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013934
Physical Location & Seq
Forward (+)
64483322 .. 64485035
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272360.1

Sequence Viewer

Length: 510 bp
ATGGGATCTGGTGCTGGAAGTTTCCTCAAAGTTCTCCTTAAAAACTTCGATGTTCTTGCTGGGCCTGTGGTTAGTTTGGTTTACCCACTGTATGCCTCAATTCGGGCTATAGAGACCAAATCTCCAACTGATGACCGCCAATGGCTTACGTATTGGATTCTCTATTCTATGATTACACTCATTGAGCTCACCTTTGCCAAACTTATTGAGTGGATCCCAGTCTGGTCATATGCCAAGCTGATCTTGACTTGTTGGTTGGTCATCCCATACTTCAGTGGTGCTGCATATGTCTACGAACACTATGTCAGGCCGTTTTTCCTCAACCCACAGACAATCAACATTTGGTATGTTCCAAAGAAGAAGGATGTCTTCAGTAAGCCGGACGATATTCTAACAGCTGCAGAGAAGTACATTGAAGAGAACGGTACTGAAGCATTTGAGCAACTCATTCACAGGTCTGACAAGTCCCAAAGGAGTGGAGGATACATCTTTGATGGCTTTCATGGCTGA

Protein Analysis

169

Amino Acids

19.48

Weight (kDa)

6.29

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 29 - 105 2.1e-28 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013934)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74520 AT1G74520
fragaria_vesca FvH4_5g15180
malus_domestica MD00G1186100.v1.1 MD06G1191500.v1.1 MD14G1197900.v1.1
prunus_persica Prupe.5G190000_v2.0.a1 Prupe.5G190000_v2.0.a1
pyrus_communis pycom06g16960
rosa_chinensis RchiOBHm_Chr7g0180981
rosa_multiflora Rmu_sc0004056.1_g000003
rosa_roxburghii Rroxscaffold_3G00272360
rosa_rugosa Rorug06G0438100 Rorug06G0438200
rosa_samantha Rh7CG040700 Rh7DG039100
rosa_wichuraiana Rw7G003250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 291
AciI CCGC 1 cut(s) 136
AclWI GGATC 3 cut(s) 13, 208, 221
AcuI CTGAAG 3 cut(s) 256, 355, 450
AfaI GTAC 2 cut(s) 410, 427
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 1 cut(s) 416
AluBI AGCT 3 cut(s) 187, 238, 398
AluI AGCT 3 cut(s) 187, 238, 398
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 3 cut(s) 13, 208, 221
AoxI GGCC 2 cut(s) 62, 308
ApeKI GCWGC 2 cut(s) 281, 398
ArsI GACNNNNNNTTYG 2 cut(s) 288, 320
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 181
BamHI GGATCC 1 cut(s) 213
BanII GRGCYC 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 268, 385
BccI CCATC 1 cut(s) 488
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciVI GTATCC 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 2 cut(s) 108, 399
BfuI GTATCC 1 cut(s) 476
BisI GCNGC 2 cut(s) 282, 399
BlsI GCNGC 2 cut(s) 283, 400
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 215
BmrI ACTGGG 1 cut(s) 212
BmuI ACTGGG 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 361
BsaAI YACGTR 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse1I ACTGG 1 cut(s) 218
BseGI GGATG 2 cut(s) 261, 370
BseLI CCNNNNNNNGG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 218
BseXI GCAGC 2 cut(s) 268, 385
BseYI CCCAGC 1 cut(s) 59
BshFI GGCC 2 cut(s) 64, 310
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 451
BsnI GGCC 2 cut(s) 64, 310
Bso31I GGTCTC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 3 cut(s) 5, 213, 240
BspACI CCGC 1 cut(s) 136
BspANI GGCC 2 cut(s) 64, 310
BspLI GGNNCC 1 cut(s) 215
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 3 cut(s) 13, 208, 221
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 218
BssMI GATC 3 cut(s) 5, 213, 240
Bst4CI ACNGT 2 cut(s) 90, 425
Bst6I CTCTTC 1 cut(s) 411
BstBAI YACGTR 1 cut(s) 150
BstF5I GGATG 2 cut(s) 261, 370
BstKTI GATC 3 cut(s) 8, 216, 243
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 3 cut(s) 5, 213, 240
BstMWI GCNNNNNNNGC 1 cut(s) 504
BstSFI CTRYAG 2 cut(s) 108, 399
BstSNI TACGTA 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 268, 385
BstV2I GAAGAC 1 cut(s) 361
BstX2I RGATCY 2 cut(s) 5, 213
BstXI CCANNNNNNTGG 1 cut(s) 476
BstYI RGATCY 2 cut(s) 5, 213
BsuI GTATCC 1 cut(s) 476
BsuRI GGCC 2 cut(s) 64, 310
BtsCI GGATG 2 cut(s) 261, 370
BtsIMutI CAGTG 2 cut(s) 86, 280
Cfr13I GGNCC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 409, 426
CviAII CATG 1 cut(s) 503
CviQI GTAC 2 cut(s) 409, 426
DpnI GATC 3 cut(s) 7, 215, 242
DpnII GATC 3 cut(s) 5, 213, 240
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 187
Eco105I TACGTA 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 107
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 3 cut(s) 256, 355, 450
EcoICRI GAGCTC 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 1 cut(s) 506
FalI AAGNNNNNCTT 6 cut(s) 21, 53, 227, 259, 353, 385
FaqI GGGAC 1 cut(s) 451
FatI CATG 1 cut(s) 502
FauNDI CATATG 2 cut(s) 229, 286
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 282, 399
FokI GGATG 2 cut(s) 248, 377
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 282, 399
GluI GCNGC 2 cut(s) 282, 399
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 2 cut(s) 64, 310
HapII CCGG 1 cut(s) 380
Hin1II CATG 1 cut(s) 506
HinfI GANTC 1 cut(s) 157
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 2 cut(s) 82, 292
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 2 cut(s) 82, 292
HpyAV CCTTC 1 cut(s) 355
HpyCH4III ACNGT 2 cut(s) 90, 425
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 2 cut(s) 284, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 504
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 1 cut(s) 506
Kzo9I GATC 3 cut(s) 5, 213, 240
LpnPI CCDG 7 cut(s) 45, 78, 208, 231, 292, 393, 439
Lsp1109I GCAGC 2 cut(s) 268, 385
MaeII ACGT 1 cut(s) 149
MalI GATC 3 cut(s) 7, 215, 242
MboI GATC 3 cut(s) 5, 213, 240
MboII GAAGA 3 cut(s) 361, 370, 428
MflI RGATCY 2 cut(s) 5, 213
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 4 cut(s) 35, 106, 329, 473
MseI TTAA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 504
NdeI CATATG 2 cut(s) 229, 286
NdeII GATC 3 cut(s) 5, 213, 240
NlaIII CATG 1 cut(s) 506
NlaIV GGNNCC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 157
PkrI GCNGC 2 cut(s) 283, 400
Ppu21I YACGTR 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 215
PspPI GGNCC 1 cut(s) 62
PstI CTGCAG 1 cut(s) 403
PsuI RGATCY 2 cut(s) 5, 213
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 2 cut(s) 410, 427
RsaNI GTAC 2 cut(s) 409, 426
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 2 cut(s) 282, 399
Sau3AI GATC 3 cut(s) 5, 213, 240
Sau96I GGNCC 1 cut(s) 62
SduI GDGCHC 1 cut(s) 189
SetI ASST 6 cut(s) 152, 189, 194, 240, 400, 458
SfcI CTRYAG 2 cut(s) 108, 399
SnaBI TACGTA 1 cut(s) 150
Sse9I AATT 1 cut(s) 99
SsiI CCGC 1 cut(s) 136
SstI GAGCTC 1 cut(s) 189
TaaI ACNGT 2 cut(s) 90, 425
TaiI ACGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 48
TasI AATT 1 cut(s) 99
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 2 cut(s) 93, 280
TseI GCWGC 2 cut(s) 281, 398
TspDTI ATGAA 1 cut(s) 491
TspRI CASTG 2 cut(s) 93, 280
XmiI GTMKAC 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.