FvH4_5g15480

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014742
Physical Location & Seq
Forward (+)
8744997 .. 8746412
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15480.t1

Sequence Viewer

Length: 762 bp
ATGAGTAACACTCCAAGTGAATTCTATGACATTGGCAAGAAAGCCACAGATCTTCTTCATAAGGACTATGCTCATCAACCACGCTCGCCAATTCATTTTGATTATCGTAGCCTCAAGTCGAATACTGATATCTCATGCAGAGTTAAAGATATTGTTCCTGGACTGAGCACCCTTTTCAGTTTTTTCATGCCAAATTATGGCAAGGTGGATCTAGAATACCTAAATGGTCTTGCTGGTATTAGTGGAGGCATTGGACTGATACGAAACGATGCTCTTGGAAGATTTGATCCGATAGCAAACCTGTCAGGTGTTATAGGACGAGACGCTCTTTTCTCCCTCGGAACTGACCTTGCATTTGACATATCTACAGGGGCATTCAACAAAATCAATGCTGGTTTGAGCTTGAACAGTGGCTTCTTTGATGCTTCCTTGTCCTTAGATAGGTTTGACAGCTTGACAGCATCATGCCACTTCATGGTGAACCCACAGACCAAGACAGCCATTGCAGCAGAGTTAAAACACAGGTTTTCAATCAACGACACTGCACTCGCAATCGGCGCCCAGCATGCGTTTACACCCTCCACACTGGCCAAGGGTCGAATTAGCACACATGGCAAGGTTGGGGCTCTTATACGGCAAGGATTGTGGCAGAGGATTTTCCTGTCTATATCTGGAGAAGTGGATTTTACAGACACAAGGCCAAATGCAGCTCTGCAAATCGGATTATCTTTGGCTGTTAGGCTCCAAATGAATATCATTTAG

Protein Analysis

254

Amino Acids

27.44

Weight (kDa)

8.54

Isoelectric Point (pI)

29.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 39 - 231 2.4e-30 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15480
malus_domestica MD06G1188300.v1.1 MD14G1194800.v1.1
prunus_persica Prupe.5G186500_v2.0.a1
pyrus_communis pycom14g16180
rosa_chinensis RchiOBHm_Chr7g0180611
rosa_laevigata RLG00000005250
rosa_multiflora Rmu_sc0005690.1_g000021 Rmu_sc0023372.1_g000001 Rmu_sc0023372.1_g000002
rosa_roxburghii Rroxscaffold_3G00272740
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035600 Rh7BG035500 Rh7CG037200 Rh7DG035800
rosa_wichuraiana Rw7G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 2 cut(s) 197, 475
AclWI GGATC 2 cut(s) 216, 281
AcoI YGGCCR 1 cut(s) 588
AcsI RAATTY 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 558
AfiI CCNNNNNNNGG 3 cut(s) 197, 441, 475
AgsI TTSAA 3 cut(s) 379, 406, 531
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 3 cut(s) 402, 453, 710
AluI AGCT 3 cut(s) 402, 453, 710
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 2 cut(s) 216, 281
AoxI GGCC 2 cut(s) 588, 698
ApeKI GCWGC 2 cut(s) 506, 707
ApoI RAATTY 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AspLEI GCGC 1 cut(s) 560
AsuHPI GGTGA 1 cut(s) 490
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 628
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 2 cut(s) 518, 719
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 561
BglII AGATCT 1 cut(s) 49
BisI GCNGC 2 cut(s) 507, 708
BlsI GCNGC 2 cut(s) 508, 709
Bme1390I CCNGG 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 559, 743
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 3 cut(s) 259, 412, 470
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 558
BsaJI CCNNGG 2 cut(s) 337, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 441, 475
Bse1I ACTGG 1 cut(s) 591
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 337, 591
BseLI CCNNNNNNNGG 3 cut(s) 197, 441, 475
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 591
BseXI GCAGC 2 cut(s) 518, 719
BseYI CCCAGC 1 cut(s) 561
BsgI GTGCAG 1 cut(s) 528
BshFI GGCC 2 cut(s) 590, 700
BshNI GGYRCC 1 cut(s) 557
BsiHKAI GWGCWC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 197, 441, 475
BsmAI GTCTC 1 cut(s) 315
BsmBI CGTCTC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 2 cut(s) 590, 700
Bsp1286I GDGCHC 2 cut(s) 170, 628
Bsp143I GATC 3 cut(s) 49, 208, 286
BspANI GGCC 2 cut(s) 590, 700
BspCNI CTCAG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 559, 743
BspPI GGATC 2 cut(s) 216, 281
BspT107I GGYRCC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 1 cut(s) 591
BssECI CCNNGG 2 cut(s) 337, 591
BssMI GATC 3 cut(s) 49, 208, 286
BssNI GRCGYC 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 410
BstACI GRCGYC 1 cut(s) 558
BstC8I GCNNGC 2 cut(s) 86, 567
BstDEI CTNAG 2 cut(s) 164, 436
BstENI CCTNNNNNAGG 1 cut(s) 439
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 1 cut(s) 560
BstKTI GATC 3 cut(s) 52, 211, 289
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 3 cut(s) 49, 208, 286
BstMWI GCNNNNNNNGC 4 cut(s) 506, 557, 566, 612
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 569
BstSCI CCNGG 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 518, 719
BstX2I RGATCY 2 cut(s) 49, 208
BstYI RGATCY 2 cut(s) 49, 208
BsuRI GGCC 2 cut(s) 590, 700
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 3 cut(s) 415, 540, 584
Cac8I GCNNGC 2 cut(s) 86, 567
CfoI GCGC 1 cut(s) 560
CseI GACGC 1 cut(s) 332
CspCI CAANNNNNGTGG 2 cut(s) 626, 661
CviAII CATG 6 cut(s) 135, 187, 465, 475, 566, 611
DdeI CTNAG 2 cut(s) 164, 436
DinI GGCGCC 1 cut(s) 559
DpnI GATC 3 cut(s) 51, 210, 288
DpnII GATC 3 cut(s) 49, 208, 286
EaeI YGGCCR 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 628
Eco32I GATATC 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 439
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 157
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 628
EgeI GGCGCC 1 cut(s) 559
EheI GGCGCC 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 591
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 6 cut(s) 138, 190, 468, 478, 569, 614
FatI CATG 6 cut(s) 134, 186, 464, 474, 565, 610
Fnu4HI GCNGC 2 cut(s) 507, 708
FriOI GRGCYC 1 cut(s) 628
Fsp4HI GCNGC 2 cut(s) 507, 708
FspBI CTAG 1 cut(s) 212
GlaI GCGC 1 cut(s) 559
GluI GCNGC 2 cut(s) 507, 708
GsaI CCCAGC 1 cut(s) 565
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 2 cut(s) 590, 700
HgaI GACGC 1 cut(s) 332
HhaI GCGC 1 cut(s) 560
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 6 cut(s) 138, 190, 468, 478, 569, 614
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 2 cut(s) 481, 573
Hpy188I TCNGA 3 cut(s) 291, 341, 722
Hpy188III TCNNGA 2 cut(s) 212, 672
Hpy8I GTNNAC 2 cut(s) 481, 573
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 6 cut(s) 138, 353, 506, 545, 707, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 557, 566, 612
HpyF3I CTNAG 2 cut(s) 164, 436
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 6 cut(s) 138, 190, 468, 478, 569, 614
HspAI GCGC 1 cut(s) 558
KasI GGCGCC 1 cut(s) 557
Kzo9I GATC 3 cut(s) 49, 208, 286
LmnI GCTCC 1 cut(s) 747
Lsp1109I GCAGC 2 cut(s) 518, 719
LweI GCATC 3 cut(s) 259, 412, 470
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 5
MalI GATC 3 cut(s) 51, 210, 288
MboI GATC 3 cut(s) 49, 208, 286
MboII GAAGA 3 cut(s) 44, 47, 291
MflI RGATCY 2 cut(s) 49, 208
MhlI GDGCHC 2 cut(s) 170, 628
MlsI TGGCCA 1 cut(s) 590
MluCI AATT 4 cut(s) 20, 90, 193, 600
MluNI TGGCCA 1 cut(s) 590
Mly113I GGCGCC 1 cut(s) 558
MnlI CCTC 5 cut(s) 122, 239, 347, 589, 645
Mox20I TGGCCA 1 cut(s) 590
MscI TGGCCA 1 cut(s) 590
MseI TTAA 2 cut(s) 144, 515
Msp20I TGGCCA 1 cut(s) 590
MspR9I CCNGG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 4 cut(s) 506, 557, 566, 612
NarI GGCGCC 1 cut(s) 558
NdeII GATC 3 cut(s) 49, 208, 286
NlaIII CATG 6 cut(s) 138, 190, 468, 478, 569, 614
NlaIV GGNNCC 2 cut(s) 559, 743
NspI RCATGY 1 cut(s) 569
PaeI GCATGC 1 cut(s) 569
PctI GAATGC 1 cut(s) 374
PflMI CCANNNNNTGG 2 cut(s) 197, 475
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 2 cut(s) 508, 709
PluTI GGCGCC 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 561
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 559, 743
PsuI RGATCY 2 cut(s) 49, 208
SaqAI TTAA 2 cut(s) 144, 515
SatI GCNGC 2 cut(s) 507, 708
Sau3AI GATC 3 cut(s) 49, 208, 286
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 2 cut(s) 170, 628
SfaNI GCATC 3 cut(s) 259, 412, 470
SfcI CTRYAG 1 cut(s) 366
SfoI GGCGCC 1 cut(s) 559
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SphI GCATGC 1 cut(s) 569
Sse9I AATT 4 cut(s) 20, 90, 193, 600
SspDI GGCGCC 1 cut(s) 557
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 2 cut(s) 119, 598
TasI AATT 4 cut(s) 20, 90, 193, 600
Tru1I TTAA 2 cut(s) 144, 515
Tru9I TTAA 2 cut(s) 144, 515
TscAI CASTG 3 cut(s) 415, 547, 591
TseI GCWGC 2 cut(s) 506, 707
TspDTI ATGAA 4 cut(s) 47, 83, 175, 463
TspRI CASTG 3 cut(s) 415, 547, 591
Van91I CCANNNNNTGG 2 cut(s) 197, 475
XagI CCTNNNNNAGG 1 cut(s) 439
XapI RAATTY 1 cut(s) 20
XbaI TCTAGA 1 cut(s) 211
XceI RCATGY 1 cut(s) 569
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.