MD14G1194800.v1.1

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014742
Physical Location & Seq
Reverse (-)
28575609 .. 28576963
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1194800.v1.1.491

Sequence Viewer

Length: 732 bp
ATGAGTAACACTTCGGGTGTGCGCTACAACATTGGCAAGGATGCCAGAGATCTTCTCTACAAGGACTATGTTCATGAACTGCCAATTCGGTTTGAATCCAAAGACTTCAACTGGATCAGTGACATCTCGTGCAAAGTTAATTTGATTGCTCCTGGACTCAGCACCCTTTTCAGTTTTAACGTGCCGGATTATGGCCAGATGGAACTGCAATACGAAAATGGTGTTGCCGAGATTGCTGGAGGAATCGGGTTGAAAAGAAATCCGCTGAAAATGTGTGAACCGGTTGCACTCTTTTCTGCAGTGATAGGAGGCAGTTCTGTGTGCTCCATTGGAACCGACCTTGCCTTCGACATATCTGCGGGAGCATTAGCAAAATTCAGTGCCGGTTTCAGCTTGAACAGTGACTTTGTTTCTGCTTCCTTATCCTTGGACAAGCTTGACACTGTAAAAGCTTCATGCTTCACCATGGTCAACCCCCTGAGCAACACAGCCCTTGGAGCAGAGTTGAAGCACCGGTTTTCAACCAACGATAGTGCCCTTGCTGTTGGTGCCCAGCACGCGTTTTTTCCATCTACACTGGCGAAGGCTCGAGTTAGCACACACGGGAATGTTGGCGTTCTTATACGGCAAGGGTTTTGGCAGAAATTGTTCCTGTCTATGTCCGGAGAAGTGGATTTTATGGGCGTACAGAGAAGTCCAAGATTTGGACTCTCTCTTTCTGTTAGGCTGTGA

Protein Analysis

244

Amino Acids

26.21

Weight (kDa)

7.68

Isoelectric Point (pI)

24.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 47 - 236 2.5e-29 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15480
malus_domestica MD06G1188300.v1.1 MD14G1194800.v1.1
prunus_persica Prupe.5G186500_v2.0.a1
pyrus_communis pycom14g16180
rosa_chinensis RchiOBHm_Chr7g0180611
rosa_laevigata RLG00000005250
rosa_multiflora Rmu_sc0005690.1_g000021 Rmu_sc0023372.1_g000001 Rmu_sc0023372.1_g000002
rosa_roxburghii Rroxscaffold_3G00272740
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035600 Rh7BG035500 Rh7CG037200 Rh7DG035800
rosa_wichuraiana Rw7G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 548
AccB7I CCANNNNNTGG 1 cut(s) 704
AccII CGCG 1 cut(s) 560
AccIII TCCGGA 1 cut(s) 662
AciI CCGC 2 cut(s) 263, 359
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 1 cut(s) 374
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 2 cut(s) 191, 704
AflIII ACRYGT 1 cut(s) 558
AgeI ACCGGT 2 cut(s) 280, 513
AgsI TTSAA 6 cut(s) 95, 109, 253, 397, 508, 522
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 3 cut(s) 393, 436, 452
AluI AGCT 3 cut(s) 393, 436, 452
Alw21I GWGCWC 1 cut(s) 326
AlwI GGATC 1 cut(s) 122
Ama87I CYCGRG 1 cut(s) 588
Aor13HI TCCGGA 1 cut(s) 662
AoxI GGCC 1 cut(s) 193
ApoI RAATTY 1 cut(s) 374
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AsiGI ACCGGT 2 cut(s) 280, 513
Asp700I GAANNNNTTC 1 cut(s) 647
AspLEI GCGC 1 cut(s) 24
AsuHPI GGTGA 1 cut(s) 454
AvaI CYCGRG 1 cut(s) 588
BaeGI GKGCMC 2 cut(s) 538, 553
BalI TGGCCA 1 cut(s) 195
BanI GGYRCC 1 cut(s) 548
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 326
BccI CCATC 2 cut(s) 193, 577
BceAI ACGGC 1 cut(s) 641
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 297
BglII AGATCT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 588
BmiI GGNNCC 2 cut(s) 334, 550
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 1 cut(s) 31
BplI GAGNNNNNCTC 2 cut(s) 39, 71
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 426, 465, 493
BsaWI WCCGGW 3 cut(s) 280, 513, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 704
Bse118I RCCGGY 3 cut(s) 280, 383, 513
Bse1I ACTGG 2 cut(s) 116, 582
BseAI TCCGGA 1 cut(s) 662
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 426, 465, 493
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 2 cut(s) 191, 704
BseMII CTCAG 2 cut(s) 172, 470
BseNI ACTGG 2 cut(s) 116, 582
BseSI GKGCMC 2 cut(s) 538, 553
BseYI CCCAGC 1 cut(s) 552
Bsh1236I CGCG 1 cut(s) 560
BshFI GGCC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 548
BshTI ACCGGT 2 cut(s) 280, 513
BsiHKAI GWGCWC 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 5 cut(s) 185, 281, 384, 514, 663
BslI CCNNNNNNNGG 2 cut(s) 191, 704
BsnI GGCC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 3 cut(s) 326, 538, 553
Bsp13I TCCGGA 1 cut(s) 662
Bsp143I GATC 2 cut(s) 49, 114
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 2 cut(s) 263, 359
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 2 cut(s) 171, 471
BspEI TCCGGA 1 cut(s) 662
BspFNI CGCG 1 cut(s) 560
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 334, 550
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 548
BsrFI RCCGGY 3 cut(s) 280, 383, 513
BsrI ACTGG 2 cut(s) 116, 582
BssAI RCCGGY 3 cut(s) 280, 383, 513
BssECI CCNNGG 3 cut(s) 426, 465, 493
BssMI GATC 2 cut(s) 49, 114
BssSI CACGAG 1 cut(s) 127
BssT1I CCWWGG 3 cut(s) 426, 465, 493
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 401, 445
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 158, 479
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 1 cut(s) 46
BstFNI CGCG 1 cut(s) 560
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 2 cut(s) 52, 117
BstMBI GATC 2 cut(s) 49, 114
BstMWI GCNNNNNNNGC 4 cut(s) 233, 497, 548, 557
BstNI CCWGG 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 2 cut(s) 538, 553
BstUI CGCG 1 cut(s) 560
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 195
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 6 cut(s) 124, 306, 385, 406, 441, 575
Cac8I GCNNGC 1 cut(s) 558
CciI TCATGA 1 cut(s) 73
CfoI GCGC 1 cut(s) 24
Cfr10I RCCGGY 3 cut(s) 280, 383, 513
Csp6I GTAC 1 cut(s) 686
CspAI ACCGGT 2 cut(s) 280, 513
CviAII CATG 3 cut(s) 74, 456, 466
CviJI RGCY 7 cut(s) 195, 393, 436, 452, 491, 587, 727
CviKI_1 RGCY 7 cut(s) 195, 393, 436, 452, 491, 587, 727
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 2 cut(s) 158, 479
DpnI GATC 2 cut(s) 51, 116
DpnII GATC 2 cut(s) 49, 114
EaeI YGGCCR 1 cut(s) 193
Eco130I CCWWGG 3 cut(s) 426, 465, 493
Eco88I CYCGRG 1 cut(s) 588
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 3 cut(s) 426, 465, 493
ErhI CCWWGG 3 cut(s) 426, 465, 493
FaeI CATG 3 cut(s) 77, 459, 469
FaiI YATR 9 cut(s) 69, 75, 192, 353, 457, 467, 623, 659, 680
FatI CATG 3 cut(s) 73, 455, 465
FauI CCCGC 1 cut(s) 352
FokI GGATG 1 cut(s) 53
GlaI GCGC 1 cut(s) 23
GsaI CCCAGC 1 cut(s) 556
GsuI CTGGAG 1 cut(s) 258
HaeIII GGCC 1 cut(s) 195
HapII CCGG 5 cut(s) 185, 281, 384, 514, 663
HhaI GCGC 1 cut(s) 24
Hin1II CATG 3 cut(s) 77, 459, 469
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 2 cut(s) 434, 450
HinfI GANTC 4 cut(s) 95, 156, 243, 708
HpaII CCGG 5 cut(s) 185, 281, 384, 514, 663
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 2 cut(s) 278, 472
Hpy188III TCNNGA 2 cut(s) 74, 663
Hpy8I GTNNAC 2 cut(s) 278, 472
HpyAV CCTTC 2 cut(s) 355, 577
HpyCH4III ACNGT 2 cut(s) 401, 445
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 4 cut(s) 132, 208, 287, 299
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 497, 548, 557
HpyF3I CTNAG 2 cut(s) 158, 479
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 3 cut(s) 77, 459, 469
HspAI GCGC 1 cut(s) 22
Kpn2I TCCGGA 1 cut(s) 662
Kzo9I GATC 2 cut(s) 49, 114
LmnI GCTCC 4 cut(s) 154, 329, 362, 497
LweI GCATC 1 cut(s) 31
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 3 cut(s) 5, 119, 401
MalI GATC 2 cut(s) 51, 116
MboI GATC 2 cut(s) 49, 114
MboII GAAGA 1 cut(s) 44
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 3 cut(s) 326, 538, 553
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 4 cut(s) 84, 139, 374, 644
MluI ACGCGT 1 cut(s) 558
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 2 cut(s) 150, 702
MnlI CCTC 2 cut(s) 233, 302
Mox20I TGGCCA 1 cut(s) 195
MroI TCCGGA 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 647
MscI TGGCCA 1 cut(s) 195
MseI TTAA 2 cut(s) 138, 177
MslI CAYNNNNRTG 1 cut(s) 606
Msp20I TGGCCA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 5 cut(s) 185, 281, 384, 514, 663
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 4 cut(s) 233, 497, 548, 557
NcoI CCATGG 1 cut(s) 465
NdeII GATC 2 cut(s) 49, 114
NlaIII CATG 3 cut(s) 77, 459, 469
NlaIV GGNNCC 2 cut(s) 334, 550
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 2 cut(s) 119, 401
PaeR7I CTCGAG 1 cut(s) 588
PagI TCATGA 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 2 cut(s) 95, 243
PflMI CCANNNNNTGG 1 cut(s) 704
PfoI TCCNGGA 1 cut(s) 151
PinAI ACCGGT 2 cut(s) 280, 513
PleI GAGTC 2 cut(s) 150, 702
PpsI GAGTC 2 cut(s) 150, 702
Psp6I CCWGG 1 cut(s) 151
PspFI CCCAGC 1 cut(s) 552
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 2 cut(s) 334, 550
PspXI VCTCGAGB 1 cut(s) 588
PstI CTGCAG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
RseI CAYNNNNRTG 1 cut(s) 606
SaqAI TTAA 2 cut(s) 138, 177
Sau3AI GATC 2 cut(s) 49, 114
SchI GAGTC 2 cut(s) 150, 702
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 3 cut(s) 326, 538, 553
SetI ASST 5 cut(s) 183, 342, 395, 438, 454
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 297
Sfr274I CTCGAG 1 cut(s) 588
SlaI CTCGAG 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 606
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 4 cut(s) 84, 139, 374, 644
SsiI CCGC 2 cut(s) 263, 359
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 3 cut(s) 426, 465, 493
TaaI ACNGT 2 cut(s) 401, 445
TaiI ACGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 348, 589
TasI AATT 4 cut(s) 84, 139, 374, 644
TfiI GAWTC 2 cut(s) 95, 243
Tru1I TTAA 2 cut(s) 138, 177
Tru9I TTAA 2 cut(s) 138, 177
TscAI CASTG 6 cut(s) 124, 306, 385, 406, 448, 582
TseFI GTSAC 2 cut(s) 119, 401
Tsp45I GTSAC 2 cut(s) 119, 401
TspDTI ATGAA 3 cut(s) 62, 90, 444
TspRI CASTG 6 cut(s) 124, 306, 385, 406, 448, 582
Van91I CCANNNNNTGG 1 cut(s) 704
XapI RAATTY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.