Rroxscaffold_3G00272740

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014742
Physical Location & Seq
Forward (+)
64737790 .. 64739239
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272740.1

Sequence Viewer

Length: 780 bp
ATGATTAACACTCCAGGTGAATTCTATGACATTGGCAAGAAGGCCAGAGATCTTCTGCATAAGGACTATGCTCATCAACCACGCGTGCCAATTCATTTTGATTATCAAAGCCTCCTCAAGTCAAATACTGATATCTCATGCAGAGTTAAAGATATTGTTCCTATTGTTCCTGAACTCAGCACCCTTTTCAGTTTTGTCACGCCAAATTCTGGCAAGGTGGAGCTGGAATACCTAAATGGTCTTACTGGTATTAGTGGAGGCATTGGACTGATACAAAACGATGCCCTTAGAAGATTTGAACCAATTGCAAACCTTTCAGGTGTGATAGGACGAGGCGCTCTTTTCTCCTTTGGAACTGACCTTGCCTTTGATATATCTACAGGGCAATTCAACAAATTCAATGCTGGTTTGAGCTTGAATAGTGACTTTGTTAATGCTTCCTTGTCCTTGGATAAGCTTGAGAGCTTGAAAGCTTCATGCCACTGCTTGGTGAACCCACTGACCAAGACGGCCATTGCAGCAGAGTTGAAACACAGCTTTTCAGGCAACGACACTGCACTTGTAATCGGCGCCCAGCACGCATTTAACCCCTCCACACTGGTCAAGGGTCGAATTAGCACACACGGCAAGGTTGGTGCTCTTATACGGCAAGGATTGTGGGAGAGGATTTTCCTGTCTCTATCTGGAGAAGTGGATTTTACAGACACAAATCCAAATCCAAATGCAACTCTGAAAATCGGATTATCTCTGGCTGTTAGGCTCCAAATGAGTATCATTTAG

Protein Analysis

259

Amino Acids

28.04

Weight (kDa)

7.76

Isoelectric Point (pI)

20.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 54 - 237 1.1e-31 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15480
malus_domestica MD06G1188300.v1.1 MD14G1194800.v1.1
prunus_persica Prupe.5G186500_v2.0.a1
pyrus_communis pycom14g16180
rosa_chinensis RchiOBHm_Chr7g0180611
rosa_laevigata RLG00000005250
rosa_multiflora Rmu_sc0005690.1_g000021 Rmu_sc0023372.1_g000001 Rmu_sc0023372.1_g000002
rosa_roxburghii Rroxscaffold_3G00272740
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035600 Rh7BG035500 Rh7CG037200 Rh7DG035800
rosa_wichuraiana Rw7G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 569
AccB7I CCANNNNNTGG 2 cut(s) 209, 487
AccII CGCG 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 3 cut(s) 20, 205, 395
AcyI GRCGYC 1 cut(s) 570
AfiI CCNNNNNNNGG 2 cut(s) 209, 487
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 6 cut(s) 299, 391, 400, 418, 469, 529
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 6 cut(s) 223, 414, 457, 465, 473, 537
AluI AGCT 6 cut(s) 223, 414, 457, 465, 473, 537
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 681
AoxI GGCC 2 cut(s) 42, 510
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 3 cut(s) 20, 205, 395
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 2 cut(s) 338, 572
AsuHPI GGTGA 2 cut(s) 29, 502
BanI GGYRCC 1 cut(s) 569
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 1 cut(s) 530
BceAI ACGGC 3 cut(s) 525, 640, 662
BciT130I CCWGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 2 cut(s) 339, 573
BglII AGATCT 1 cut(s) 49
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 571, 761
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 101, 479
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 447
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 487
Bse1I ACTGG 2 cut(s) 250, 603
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 447
BseLI CCNNNNNNNGG 2 cut(s) 209, 487
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 1 cut(s) 190
BseNI ACTGG 2 cut(s) 250, 603
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 530
BseYI CCCAGC 1 cut(s) 573
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 44, 512
BshNI GGYRCC 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 640
BslI CCNNNNNNNGG 2 cut(s) 209, 487
BsmAI GTCTC 1 cut(s) 681
BsnI GGCC 2 cut(s) 44, 512
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 1 cut(s) 49
BspANI GGCC 2 cut(s) 44, 512
BspCNI CTCAG 1 cut(s) 189
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 571, 761
BspT107I GGYRCC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 513
BsrI ACTGG 2 cut(s) 250, 603
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 570
BstC8I GCNNGC 2 cut(s) 86, 579
BstDEI CTNAG 2 cut(s) 176, 287
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 2 cut(s) 339, 573
BstHHI GCGC 2 cut(s) 338, 572
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 681
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 4 cut(s) 518, 543, 578, 624
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 530
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 2 cut(s) 44, 512
BtsI GCAGTG 2 cut(s) 481, 552
BtsIMutI CAGTG 4 cut(s) 481, 497, 552, 596
Cac8I GCNNGC 2 cut(s) 86, 579
CfoI GCGC 2 cut(s) 338, 572
CspCI CAANNNNNGTGG 2 cut(s) 638, 673
CviAII CATG 2 cut(s) 138, 477
DdeI CTNAG 2 cut(s) 176, 287
DinI GGCGCC 1 cut(s) 571
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 447
Eco32I GATATC 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 447
EgeI GGCGCC 1 cut(s) 571
EheI GGCGCC 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 2 cut(s) 141, 480
FaiI YATR 7 cut(s) 27, 60, 69, 139, 374, 478, 644
FatI CATG 2 cut(s) 137, 476
Fnu4HI GCNGC 1 cut(s) 519
Fsp4HI GCNGC 1 cut(s) 519
GlaI GCGC 2 cut(s) 337, 571
GluI GCNGC 1 cut(s) 519
GsaI CCCAGC 1 cut(s) 577
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 2 cut(s) 339, 573
HaeIII GGCC 2 cut(s) 44, 512
HhaI GCGC 2 cut(s) 338, 572
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 2 cut(s) 141, 480
Hin6I GCGC 2 cut(s) 336, 570
HinP1I GCGC 2 cut(s) 336, 570
HindIII AAGCTT 2 cut(s) 455, 471
HphI GGTGA 2 cut(s) 29, 502
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 2 cut(s) 732, 740
Hpy188III TCNNGA 2 cut(s) 170, 684
Hpy8I GTNNAC 1 cut(s) 493
HpyAV CCTTC 1 cut(s) 34
HpyCH4V TGCA 6 cut(s) 58, 141, 308, 518, 557, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 543, 578, 624
HpyF3I CTNAG 2 cut(s) 176, 287
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 2 cut(s) 141, 480
HspAI GCGC 2 cut(s) 336, 570
KasI GGCGCC 1 cut(s) 569
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 2 cut(s) 220, 765
Lsp1109I GCAGC 1 cut(s) 530
LweI GCATC 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 196, 422
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 2 cut(s) 44, 303
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 640
MluCI AATT 7 cut(s) 20, 90, 205, 303, 386, 395, 612
MluI ACGCGT 1 cut(s) 82
Mly113I GGCGCC 1 cut(s) 570
MnlI CCTC 6 cut(s) 122, 125, 251, 326, 601, 657
MseI TTAA 4 cut(s) 6, 147, 432, 585
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 1 cut(s) 303
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 4 cut(s) 518, 543, 578, 624
NarI GGCGCC 1 cut(s) 570
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 141, 480
NlaIV GGNNCC 2 cut(s) 571, 761
NmuCI GTSAC 2 cut(s) 196, 422
PflMI CCANNNNNTGG 2 cut(s) 209, 487
PkrI GCNGC 1 cut(s) 520
PluTI GGCGCC 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 573
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 571, 761
PsuI RGATCY 1 cut(s) 49
SaqAI TTAA 4 cut(s) 6, 147, 432, 585
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 1 cut(s) 49
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 640
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 571
SmlI CTYRAG 2 cut(s) 116, 458
SmoI CTYRAG 2 cut(s) 116, 458
Sse9I AATT 7 cut(s) 20, 90, 205, 303, 386, 395, 612
SspDI GGCGCC 1 cut(s) 569
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 447
TaqI TCGA 1 cut(s) 610
TasI AATT 7 cut(s) 20, 90, 205, 303, 386, 395, 612
Tru1I TTAA 4 cut(s) 6, 147, 432, 585
Tru9I TTAA 4 cut(s) 6, 147, 432, 585
TscAI CASTG 4 cut(s) 488, 504, 559, 603
TseFI GTSAC 2 cut(s) 196, 422
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 2 cut(s) 196, 422
TspDTI ATGAA 2 cut(s) 83, 465
TspRI CASTG 4 cut(s) 488, 504, 559, 603
Van91I CCANNNNNTGG 2 cut(s) 209, 487
XapI RAATTY 3 cut(s) 20, 205, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.