RchiOBHm_Chr7g0180611

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014742
Physical Location & Seq
Reverse (-)
2476710 .. 2478093
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16107

Sequence Viewer

Length: 771 bp
ATGATTAACACTCCAGGTGAATTCTATGACATTGGCAAGAAGGCCAGAGATCTTCTGCATAAGGACTATGCTCATCAACCACGCGTGCCAATTCATTTTGATTATCGAAGCCTCAAGTCAAATACTGATATCTCATGCAGAGTTAAAGATATTGTTCCAATTGTTCCTGAACTCAACACCCTTTTCAGTTTTGTCACGCCAAATTCTGGCAAGGTGGAGCTGGAATACCTAAATGGTCTTGCTGGTATTAGTGGAGGCATTGGACTGATACAAAACGATGCCCTTAGAAGATTTGAACCAATTGCAAACCTTTCAGGTGTGATAGGACGAGGCGCTCTTTTCTCCTTCGGAACGGACCTTGCCTTTGACATATATACAGGGCAATTCAACAAATTCAATGCTGGTTTGAGCTTGAATAGTGACTTTGTTAATACTTCCTTGTCCTTGGATGGGCTTGACAGCTTGAAAGCTTCATGCCACTGCTTGGTGAACCCACTGACCAAGACGGCCATTGCAGCAGAGTTGAAACACAGCTTTTCAGGCAACAACACTGCACTTGTAATCGGCGCCCAGCACGCATTTGCCCCCTCCACACTGGTCAAGGGTCGAATTAGCACACACGGCAAGGTTGGTGCTCTTATACGGCAAGGATTGTGGGAGAGGATTTTCCTGTCTCTATCTGGAGAAGTGGATTTTACAGACACAAATCCAAATGCAACTCTGAAAATCGGATTATCTCTGGCTGTTAGGCTCCAAATGAGTATCATTTAG

Protein Analysis

256

Amino Acids

27.72

Weight (kDa)

8.41

Isoelectric Point (pI)

21.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 52 - 244 6.8e-31 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15480
malus_domestica MD06G1188300.v1.1 MD14G1194800.v1.1
prunus_persica Prupe.5G186500_v2.0.a1
pyrus_communis pycom14g16180
rosa_chinensis RchiOBHm_Chr7g0180611
rosa_laevigata RLG00000005250
rosa_multiflora Rmu_sc0005690.1_g000021 Rmu_sc0023372.1_g000001 Rmu_sc0023372.1_g000002
rosa_roxburghii Rroxscaffold_3G00272740
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035600 Rh7BG035500 Rh7CG037200 Rh7DG035800
rosa_wichuraiana Rw7G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 566
AccB7I CCANNNNNTGG 2 cut(s) 206, 484
AccII CGCG 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 3 cut(s) 20, 202, 392
AcyI GRCGYC 1 cut(s) 567
AfiI CCNNNNNNNGG 3 cut(s) 206, 450, 484
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 6 cut(s) 296, 388, 397, 415, 466, 526
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 5 cut(s) 220, 411, 462, 470, 534
AluI AGCT 5 cut(s) 220, 411, 462, 470, 534
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 1 cut(s) 678
AoxI GGCC 2 cut(s) 42, 507
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 3 cut(s) 20, 202, 392
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspLEI GCGC 2 cut(s) 335, 569
AspS9I GGNCC 1 cut(s) 355
AsuHPI GGTGA 2 cut(s) 29, 499
AvaII GGWCC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 1 cut(s) 527
BccI CCATC 1 cut(s) 443
BceAI ACGGC 3 cut(s) 522, 637, 659
BciT130I CCWGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 678
BfoI RGCGCY 2 cut(s) 336, 570
BglII AGATCT 1 cut(s) 49
BisI GCNGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 517
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 355
BmgT120I GGNCC 1 cut(s) 355
BmiI GGNNCC 2 cut(s) 568, 752
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 268
BpmI CTGGAG 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 444
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 450, 484
Bse1I ACTGG 1 cut(s) 600
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 1 cut(s) 454
BseLI CCNNNNNNNGG 3 cut(s) 206, 450, 484
BseMI GCAATG 1 cut(s) 510
BseNI ACTGG 1 cut(s) 600
BseXI GCAGC 1 cut(s) 527
BseYI CCCAGC 1 cut(s) 570
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 44, 509
BshNI GGYRCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 637
BslI CCNNNNNNNGG 3 cut(s) 206, 450, 484
BsmAI GTCTC 1 cut(s) 678
BsnI GGCC 2 cut(s) 44, 509
Bsp1286I GDGCHC 1 cut(s) 637
Bsp143I GATC 1 cut(s) 49
BspANI GGCC 2 cut(s) 44, 509
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 568, 752
BspT107I GGYRCC 1 cut(s) 566
BsrDI GCAATG 1 cut(s) 510
BsrI ACTGG 1 cut(s) 600
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 86, 576
BstDEI CTNAG 1 cut(s) 284
BstF5I GGATG 1 cut(s) 454
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 2 cut(s) 336, 570
BstHHI GCGC 2 cut(s) 335, 569
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 4 cut(s) 515, 540, 575, 621
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 2 cut(s) 44, 509
BtsCI GGATG 1 cut(s) 454
BtsI GCAGTG 2 cut(s) 478, 549
BtsIMutI CAGTG 4 cut(s) 478, 494, 549, 593
Cac8I GCNNGC 2 cut(s) 86, 576
CfoI GCGC 2 cut(s) 335, 569
Cfr13I GGNCC 1 cut(s) 355
CspCI CAANNNNNGTGG 2 cut(s) 635, 670
CviAII CATG 2 cut(s) 135, 474
DdeI CTNAG 1 cut(s) 284
DinI GGCGCC 1 cut(s) 568
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 444
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 355
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 444
EgeI GGCGCC 1 cut(s) 568
EheI GGCGCC 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 2 cut(s) 138, 477
FaiI YATR 9 cut(s) 27, 60, 69, 136, 371, 373, 375, 475, 641
FatI CATG 2 cut(s) 134, 473
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 1 cut(s) 461
Fsp4HI GCNGC 1 cut(s) 516
GlaI GCGC 2 cut(s) 334, 568
GluI GCNGC 1 cut(s) 516
GsaI CCCAGC 1 cut(s) 574
GsuI CTGGAG 1 cut(s) 702
HaeII RGCGCY 2 cut(s) 336, 570
HaeIII GGCC 2 cut(s) 44, 509
HhaI GCGC 2 cut(s) 335, 569
Hin1I GRCGYC 1 cut(s) 567
Hin1II CATG 2 cut(s) 138, 477
Hin6I GCGC 2 cut(s) 333, 567
HinP1I GCGC 2 cut(s) 333, 567
HindIII AAGCTT 1 cut(s) 468
HphI GGTGA 2 cut(s) 29, 499
Hpy166II GTNNAC 1 cut(s) 490
Hpy188I TCNGA 3 cut(s) 350, 723, 731
Hpy188III TCNNGA 2 cut(s) 167, 681
Hpy8I GTNNAC 1 cut(s) 490
HpyAV CCTTC 2 cut(s) 34, 355
HpyCH4V TGCA 6 cut(s) 58, 138, 305, 515, 554, 716
HpyF10VI GCNNNNNNNGC 4 cut(s) 515, 540, 575, 621
HpyF3I CTNAG 1 cut(s) 284
Hsp92I GRCGYC 1 cut(s) 567
Hsp92II CATG 2 cut(s) 138, 477
HspAI GCGC 2 cut(s) 333, 567
KasI GGCGCC 1 cut(s) 566
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 2 cut(s) 217, 756
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 268
MaeIII GTNAC 2 cut(s) 193, 419
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 2 cut(s) 44, 300
MfeI CAATTG 2 cut(s) 159, 300
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 637
MluCI AATT 8 cut(s) 20, 90, 159, 202, 300, 383, 392, 609
MluI ACGCGT 1 cut(s) 82
Mly113I GGCGCC 1 cut(s) 567
MnlI CCTC 5 cut(s) 122, 248, 323, 598, 654
MseI TTAA 3 cut(s) 6, 144, 429
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 2 cut(s) 159, 300
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 4 cut(s) 515, 540, 575, 621
NarI GGCGCC 1 cut(s) 567
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 138, 477
NlaIV GGNNCC 2 cut(s) 568, 752
NmuCI GTSAC 2 cut(s) 193, 419
PflMI CCANNNNNTGG 2 cut(s) 206, 484
PkrI GCNGC 1 cut(s) 517
PluTI GGCGCC 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 568, 752
PspPI GGNCC 1 cut(s) 355
PsuI RGATCY 1 cut(s) 49
SaqAI TTAA 3 cut(s) 6, 144, 429
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 355
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 1 cut(s) 268
SfoI GGCGCC 1 cut(s) 568
SinI GGWCC 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 8 cut(s) 20, 90, 159, 202, 300, 383, 392, 609
SspDI GGCGCC 1 cut(s) 566
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 444
TaqI TCGA 2 cut(s) 106, 607
TasI AATT 8 cut(s) 20, 90, 159, 202, 300, 383, 392, 609
Tru1I TTAA 3 cut(s) 6, 144, 429
Tru9I TTAA 3 cut(s) 6, 144, 429
TscAI CASTG 4 cut(s) 485, 501, 556, 600
TseFI GTSAC 2 cut(s) 193, 419
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 2 cut(s) 193, 419
TspDTI ATGAA 2 cut(s) 83, 462
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 4 cut(s) 485, 501, 556, 600
Van91I CCANNNNNTGG 2 cut(s) 206, 484
VpaK11BI GGWCC 1 cut(s) 355
XapI RAATTY 3 cut(s) 20, 202, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.