FvH4_5g16040

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007510
Physical Location & Seq
Forward (+)
9101995 .. 9104670
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16040.t1

Sequence Viewer

Length: 1344 bp
ATGGTGTCCTCATCTCAGAACAGCAATAAAAGCCTCCATTCCCTCCTCGCCGCAAGGCTCACCAACCTCACAATCCCGCCTTCCTCCTCCTCCTCATCTCCGCCTCAAGACTTCGACTTCAACGACGTCTTCGGCCCCCCAACCGCCTCCAGCGACGCCGCGCCGCCGCCTTTGCTAGTCATCCACAACCGCTCCCACTCCTTCGTCGGCCCCTCCCCGCGCTTCACCCCCACCTCCTCCTCCTTCCTCCGCCCCTTCGCCGCCGAATCCGACTCTGATGAAGACGAAAACGACGCCGTTTCCGAGAAAGATGAGGAACCGGCGGTGCGGAAGTTCGGGCCTTCGGATTTTGAGATTCTCAGAGTCGTCGGGAGGGGCGCGTTCGGGAAGGTGTTTCAGGTGAGGAAGAAGAGGTGTGATGGTGAGGAAGATGGCGATGGGCTTTATGCTATGAAGGTTATGAAGAAGGAGACTGTGATTAAAAAGAACCATGTGGATTACATGAAGGCTGAGAGAGATATTCTCACTAAAGTTGTCCACCCTTTCATTGTTCAGCTCCGCTACTCTTTTCAGACCAAGAGTAAGCTGTACCTGATTATGGATTTCATGAACGGAGGGCATCTGTTCTATCATTTGTACCGGCAGCGGATATTCAGTGAGGATCAGGCGAGGTTTTATACTGCGGAGATAGTGTCTGCTGTTTCACATCTTCACAAGTGCGGGATTGTGCATCGGGACCTTAAGCCGGAAAATGTTCTCATGGATGCTGATGGCCATGTGATGCTGACGGATTTTGGACTAGCAAAGGAAATTGATGAGGATAGCAGATCAAATTCAATGTGTGGAACCACGGAATATATGGCTCCGGAAATTTTGCTGTCTAAAGGGCACAACAAAGATGCAGATTGGTGGAGCATTGGCATACTTTTGTATGAGATGCTAAGTGGCCAGCCGCCATTTACGCATGCAAATAGAAAAAAACTTCAGGAGAGAATCATCAAAGAGAAAATTAAACTTCCACCTTACCTTACCACTGACGCTCACTCTTTGCTCAAAGGATTGCTACAAAAGGAACCATCTCAAAGGTTAGGCAGTGGCCCCAGTGGAGGTGATGATATCAAACGTCATAAATGGTTTAAAACAATTAACTGGAAGAAGTTGGAGGCGAGAGAACTGCTCCCAAAGTTCAAGCCAGATGTGACTGGAAAAGACTGCACGGCCAATTTTGACCGGTGCTGGACAACAATGCCTCTAGATGACTCACCAGCTCCTACACCAACAGCGGGTGAGCATTTTCAGGGATATACTTATGTGGCACCTAACACTTGGCTTTCATCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

448

Amino Acids

50.33

Weight (kDa)

6.98

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 117 - 379 6.8e-74 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 118 - 362 3.8e-40 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 194 - 278 6.1e-07 ABC1 atypical kinase-like domain
Kinase-like PF14531 212 - 361 6.8e-10 Kinase-like
Pkinase_fungal PF17667 230 - 302 2.1e-08 Fungal protein kinase
Pkinase_C PF00433 400 - 437 2.7e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 1315
AccBSI CCGCTC 1 cut(s) 192
AccII CGCG 3 cut(s) 161, 220, 380
AccIII TCCGGA 1 cut(s) 865
AclWI GGATC 1 cut(s) 669
AcoI YGGCCR 3 cut(s) 772, 946, 1218
AcsI RAATTY 2 cut(s) 832, 870
AcuI CTGAAG 1 cut(s) 968
AcyI GRCGYC 3 cut(s) 126, 156, 294
AfaI GTAC 2 cut(s) 590, 638
AfiI CCNNNNNNNGG 4 cut(s) 598, 745, 1106, 1283
AflII CTTAAG 1 cut(s) 740
AgeI ACCGGT 1 cut(s) 1230
AgsI TTSAA 3 cut(s) 121, 837, 1189
AluBI AGCT 3 cut(s) 556, 586, 1268
AluI AGCT 3 cut(s) 556, 586, 1268
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 1 cut(s) 669
Aor13HI TCCGGA 1 cut(s) 865
AoxI GGCC 7 cut(s) 133, 208, 338, 772, 946, 1096, 1218
ApeKI GCWGC 1 cut(s) 643
ApoI RAATTY 2 cut(s) 832, 870
AsiGI ACCGGT 1 cut(s) 1230
Asp700I GAANNNNTTC 1 cut(s) 753
AspLEI GCGC 3 cut(s) 163, 222, 380
AspS9I GGNCC 5 cut(s) 134, 209, 338, 736, 1097
AsuHPI GGTGA 7 cut(s) 52, 217, 412, 434, 1121, 1254, 1298
AvaII GGWCC 1 cut(s) 736
BaeGI GKGCMC 1 cut(s) 891
BalI TGGCCA 2 cut(s) 774, 948
BanI GGYRCC 1 cut(s) 1315
BbsI GAAGAC 2 cut(s) 121, 288
BbvI GCAGC 1 cut(s) 655
BccI CCATC 5 cut(s) 413, 425, 431, 764, 1084
BceAI ACGGC 2 cut(s) 281, 1233
BcgI CGANNNNNNTGC 2 cut(s) 1156, 1190
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 3 cut(s) 176, 800, 1253
BfrI CTTAAG 1 cut(s) 740
BisI GCNGC 7 cut(s) 51, 159, 164, 167, 261, 644, 953
BlsI GCNGC 7 cut(s) 52, 160, 165, 168, 262, 645, 954
Bme18I GGWCC 1 cut(s) 736
BmgT120I GGNCC 5 cut(s) 134, 209, 338, 736, 1097
BmiI GGNNCC 9 cut(s) 136, 211, 318, 737, 847, 864, 1074, 1099, 1317
BmrI ACTGGG 1 cut(s) 1095
BmsI GCATC 6 cut(s) 628, 739, 754, 771, 889, 927
BmuI ACTGGG 1 cut(s) 1095
BpiI GAAGAC 2 cut(s) 121, 288
BplI GAGNNNNNCTC 4 cut(s) 507, 539, 1161, 1193
BpmI CTGGAG 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 90
BsaHI GRCGYC 3 cut(s) 126, 156, 294
BsaJI CCNNGG 1 cut(s) 849
BsaWI WCCGGW 2 cut(s) 865, 1230
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 677, 707
Bsc4I CCNNNNNNNGG 4 cut(s) 598, 745, 1106, 1283
Bse118I RCCGGY 3 cut(s) 319, 639, 1230
Bse1I ACTGG 3 cut(s) 1101, 1154, 1207
BseAI TCCGGA 1 cut(s) 865
BseDI CCNNGG 1 cut(s) 849
BseGI GGATG 2 cut(s) 180, 769
BseLI CCNNNNNNNGG 4 cut(s) 598, 745, 1106, 1283
BseMII CTCAG 3 cut(s) 29, 373, 501
BseNI ACTGG 3 cut(s) 1101, 1154, 1207
BseRI GAGGAG 6 cut(s) 35, 76, 79, 82, 226, 229
BseSI GKGCMC 1 cut(s) 891
BseXI GCAGC 1 cut(s) 655
BsgI GTGCAG 1 cut(s) 1198
Bsh1236I CGCG 3 cut(s) 161, 220, 380
BshFI GGCC 7 cut(s) 135, 210, 340, 774, 948, 1098, 1220
BshNI GGYRCC 1 cut(s) 1315
BshTI ACCGGT 1 cut(s) 1230
BsiSI CCGG 5 cut(s) 320, 640, 746, 866, 1231
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 4 cut(s) 598, 745, 1106, 1283
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 749
BsnI GGCC 7 cut(s) 135, 210, 340, 774, 948, 1098, 1220
Bsp1286I GDGCHC 1 cut(s) 891
Bsp13I TCCGGA 1 cut(s) 865
Bsp143I GATC 2 cut(s) 661, 827
BspANI GGCC 7 cut(s) 135, 210, 340, 774, 948, 1098, 1220
BspCNI CTCAG 3 cut(s) 28, 372, 502
BspEI TCCGGA 1 cut(s) 865
BspFNI CGCG 3 cut(s) 161, 220, 380
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 9 cut(s) 136, 211, 318, 737, 847, 864, 1074, 1099, 1317
BspPI GGATC 1 cut(s) 669
BspT107I GGYRCC 1 cut(s) 1315
BspTI CTTAAG 1 cut(s) 740
BsrBI CCGCTC 1 cut(s) 192
BsrFI RCCGGY 3 cut(s) 319, 639, 1230
BsrI ACTGG 3 cut(s) 1101, 1154, 1207
BssAI RCCGGY 3 cut(s) 319, 639, 1230
BssECI CCNNGG 1 cut(s) 849
BssMI GATC 2 cut(s) 661, 827
BssNI GRCGYC 3 cut(s) 126, 156, 294
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 1 cut(s) 404
BstACI GRCGYC 3 cut(s) 126, 156, 294
BstAFI CTTAAG 1 cut(s) 740
BstC8I GCNNGC 2 cut(s) 950, 966
BstDEI CTNAG 4 cut(s) 15, 359, 510, 941
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 2 cut(s) 180, 769
BstFNI CGCG 3 cut(s) 161, 220, 380
BstHHI GCGC 3 cut(s) 163, 222, 380
BstKTI GATC 2 cut(s) 664, 830
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 2 cut(s) 661, 827
BstMWI GCNNNNNNNGC 3 cut(s) 30, 172, 961
BstNSI RCATGY 1 cut(s) 968
BstSLI GKGCMC 1 cut(s) 891
BstUI CGCG 3 cut(s) 161, 220, 380
BstV1I GCAGC 1 cut(s) 655
BstV2I GAAGAC 2 cut(s) 121, 288
BsuRI GGCC 7 cut(s) 135, 210, 340, 774, 948, 1098, 1220
BtgI CCRYGG 1 cut(s) 849
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 2 cut(s) 180, 769
BtsI GCAGTG 1 cut(s) 1099
BtsIMutI CAGTG 4 cut(s) 661, 1032, 1099, 1108
Cac8I GCNNGC 2 cut(s) 950, 966
CciI TCATGA 1 cut(s) 606
CfoI GCGC 3 cut(s) 163, 222, 380
Cfr10I RCCGGY 3 cut(s) 319, 639, 1230
Cfr13I GGNCC 5 cut(s) 134, 209, 338, 736, 1097
CseI GACGC 3 cut(s) 164, 302, 1046
Csp6I GTAC 2 cut(s) 589, 637
CspAI ACCGGT 1 cut(s) 1230
CviAII CATG 6 cut(s) 491, 502, 607, 760, 776, 965
CviQI GTAC 2 cut(s) 589, 637
DdeI CTNAG 4 cut(s) 15, 359, 510, 941
DpnI GATC 2 cut(s) 663, 829
DpnII GATC 2 cut(s) 661, 827
DraI TTTAAA 1 cut(s) 1138
EaeI YGGCCR 3 cut(s) 772, 946, 1218
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
EciI GGCGGA 2 cut(s) 90, 239
Eco32I GATATC 1 cut(s) 1117
Eco47I GGWCC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 968
EcoO109I RGGNCCY 1 cut(s) 736
EcoRV GATATC 1 cut(s) 1117
FaeI CATG 6 cut(s) 494, 505, 610, 763, 779, 968
FaqI GGGAC 1 cut(s) 749
FatI CATG 6 cut(s) 490, 501, 606, 759, 775, 964
FauI CCCGC 4 cut(s) 84, 225, 713, 1276
Fnu4HI GCNGC 7 cut(s) 51, 159, 164, 167, 261, 644, 953
FokI GGATG 2 cut(s) 167, 776
Fsp4HI GCNGC 7 cut(s) 51, 159, 164, 167, 261, 644, 953
FspBI CTAG 3 cut(s) 176, 800, 1253
GlaI GCGC 3 cut(s) 162, 221, 379
GluI GCNGC 7 cut(s) 51, 159, 164, 167, 261, 644, 953
GsuI CTGGAG 1 cut(s) 133
HaeIII GGCC 7 cut(s) 135, 210, 340, 774, 948, 1098, 1220
HapII CCGG 5 cut(s) 320, 640, 746, 866, 1231
HgaI GACGC 3 cut(s) 164, 302, 1046
HhaI GCGC 3 cut(s) 163, 222, 380
Hin1I GRCGYC 3 cut(s) 126, 156, 294
Hin1II CATG 6 cut(s) 494, 505, 610, 763, 779, 968
Hin6I GCGC 3 cut(s) 161, 220, 378
HinP1I GCGC 3 cut(s) 161, 220, 378
HinfI GANTC 6 cut(s) 266, 272, 355, 363, 993, 1259
HpaII CCGG 5 cut(s) 320, 640, 746, 866, 1231
HphI GGTGA 7 cut(s) 52, 217, 412, 434, 1121, 1254, 1298
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 7 cut(s) 18, 271, 277, 304, 346, 362, 573
Hpy188III TCNNGA 8 cut(s) 107, 370, 385, 607, 734, 866, 986, 1253
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 5 cut(s) 128, 158, 209, 296, 371
HpyAV CCTTC 9 cut(s) 90, 211, 253, 265, 351, 382, 448, 460, 499
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 2 cut(s) 126, 1123
HpyCH4V TGCA 4 cut(s) 730, 902, 968, 1215
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 172, 961
HpyF3I CTNAG 4 cut(s) 15, 359, 510, 941
HpySE526I ACGT 2 cut(s) 126, 1123
Hsp92I GRCGYC 3 cut(s) 126, 156, 294
Hsp92II CATG 6 cut(s) 494, 505, 610, 763, 779, 968
HspAI GCGC 3 cut(s) 161, 220, 378
Kpn2I TCCGGA 1 cut(s) 865
Kzo9I GATC 2 cut(s) 661, 827
LmnI GCTCC 6 cut(s) 197, 561, 868, 912, 1182, 1273
Lsp1109I GCAGC 1 cut(s) 655
LweI GCATC 6 cut(s) 628, 739, 754, 771, 889, 927
MaeI CTAG 3 cut(s) 176, 800, 1253
MaeII ACGT 2 cut(s) 126, 1123
MaeIII GTNAC 1 cut(s) 1198
MalI GATC 2 cut(s) 663, 829
MbiI CCGCTC 1 cut(s) 192
MboI GATC 2 cut(s) 661, 827
MboII GAAGA 8 cut(s) 121, 293, 418, 421, 440, 475, 701, 1165
MhlI GDGCHC 1 cut(s) 891
MlsI TGGCCA 2 cut(s) 774, 948
MluCI AATT 6 cut(s) 810, 832, 870, 1008, 1143, 1222
MluNI TGGCCA 2 cut(s) 774, 948
MlyI GAGTC 3 cut(s) 266, 372, 1253
MmeI TCCRAC 2 cut(s) 294, 1140
Mox20I TGGCCA 2 cut(s) 774, 948
MroI TCCGGA 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 753
MscI TGGCCA 2 cut(s) 774, 948
MseI TTAA 5 cut(s) 480, 741, 1011, 1137, 1146
Msp20I TGGCCA 2 cut(s) 774, 948
MspA1I CMGCKG 2 cut(s) 646, 1283
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 5 cut(s) 320, 640, 746, 866, 1231
MvnI CGCG 3 cut(s) 161, 220, 380
MwoI GCNNNNNNNGC 3 cut(s) 30, 172, 961
NdeII GATC 2 cut(s) 661, 827
NlaIII CATG 6 cut(s) 494, 505, 610, 763, 779, 968
NlaIV GGNNCC 9 cut(s) 136, 211, 318, 737, 847, 864, 1074, 1099, 1317
NmuCI GTSAC 1 cut(s) 1198
NspI RCATGY 1 cut(s) 968
PaeI GCATGC 1 cut(s) 968
PagI TCATGA 1 cut(s) 606
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 300
PdmI GAANNNNTTC 1 cut(s) 753
PfeI GAWTC 3 cut(s) 266, 355, 993
PinAI ACCGGT 1 cut(s) 1230
PkrI GCNGC 7 cut(s) 52, 160, 165, 168, 262, 645, 954
PleI GAGTC 3 cut(s) 266, 371, 1253
PpsI GAGTC 3 cut(s) 266, 371, 1253
PpuMI RGGWCCY 1 cut(s) 736
Psp5II RGGWCCY 1 cut(s) 736
PspN4I GGNNCC 9 cut(s) 136, 211, 318, 737, 847, 864, 1074, 1099, 1317
PspPI GGNCC 5 cut(s) 134, 209, 338, 736, 1097
PspPPI RGGWCCY 1 cut(s) 736
RsaI GTAC 2 cut(s) 590, 638
RsaNI GTAC 2 cut(s) 589, 637
SaqAI TTAA 5 cut(s) 480, 741, 1011, 1137, 1146
SatI GCNGC 7 cut(s) 51, 159, 164, 167, 261, 644, 953
Sau3AI GATC 2 cut(s) 661, 827
Sau96I GGNCC 5 cut(s) 134, 209, 338, 736, 1097
SchI GAGTC 3 cut(s) 266, 372, 1253
SduI GDGCHC 1 cut(s) 891
SfaNI GCATC 6 cut(s) 628, 739, 754, 771, 889, 927
SinI GGWCC 1 cut(s) 736
SmlI CTYRAG 2 cut(s) 105, 740
SmoI CTYRAG 2 cut(s) 105, 740
SphI GCATGC 1 cut(s) 968
Sse9I AATT 6 cut(s) 810, 832, 870, 1008, 1143, 1222
SspMI CTAG 3 cut(s) 176, 800, 1253
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 2 cut(s) 129, 1126
TaqI TCGA 1 cut(s) 114
TasI AATT 6 cut(s) 810, 832, 870, 1008, 1143, 1222
TauI GCSGC 6 cut(s) 53, 161, 166, 169, 263, 955
TfiI GAWTC 3 cut(s) 266, 355, 993
Tru1I TTAA 5 cut(s) 480, 741, 1011, 1137, 1146
Tru9I TTAA 5 cut(s) 480, 741, 1011, 1137, 1146
TscAI CASTG 4 cut(s) 661, 1039, 1099, 1108
TseFI GTSAC 1 cut(s) 1198
TseI GCWGC 1 cut(s) 643
Tsp45I GTSAC 1 cut(s) 1198
TspDTI ATGAA 8 cut(s) 294, 467, 476, 518, 535, 595, 623, 1323
TspGWI ACGGA 3 cut(s) 627, 803, 866
TspRI CASTG 4 cut(s) 661, 1039, 1099, 1108
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 736
XapI RAATTY 2 cut(s) 832, 870
XbaI TCTAGA 1 cut(s) 1252
XceI RCATGY 1 cut(s) 968
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XmnI GAANNNNTTC 1 cut(s) 753
XspI CTAG 3 cut(s) 176, 800, 1253
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.