Rw7G002490

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007510
Physical Location & Seq
Reverse (-)
2052462 .. 2054782
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002490.1

Sequence Viewer

Length: 1365 bp
ATGGTGTCCTCCTCTCAGAACAATAACAAAACCCTCCATTCCCTCCTTGCCTCAAACCTCACCAAGCTCACGATCCCACCTTCCTCCTCTTCTTCTCCGCCTCAAGACTTCGACTTTAACGACGTCTTCGGCCCCCCAACCGCCTCCAACGACGGCGGCGCGTCCACTTCATTCTCGCCGTCGTTACTCGTCATCCACAACCGCTCCCACTCCTTCGTCGGCCCCTCCCCGCGCTTCACTCCCACCTCGTCCTCCTCCCTCCTCCCCTTCACCGCCGCCGAATCCGACTCCGAGGAAGACGAAAACGACGCCGTTTCCGAAACCGTGAAAGATGAGGAATCGGCGGTTCGGAAGTTCGGGCCTTGGGATTTCGAGATTCTCAGAGTCGTCGGCAAGGGCGCGTTCGGGAAGGTGTTTCAGGTGAGGAAGAAGAGCAGTGATGGCGATGAAGATGGTGATGGGCTTTATGCCATGAAGGTCATGAGGAAGGAGACTGTTATCAAGAAGAACCATGTGGATTACATGAAGGCTGAGAGGGACATTCTCACCAAAGTTGTGCACCCTTTTATTGTTCAGCTCCGCTACTCTTTTCAGACCAAGTGTAAGCTGTACTTGATCATGGATTTCATGAATGGAGGGCATTTGTTCTATCATTTGTACCGGCAGGGGATATTCAGTGAGGATCAGGCGAGGTTTTACACTGCTGAGATAGTGTCTGCTGTTTCACATCTTCACAAATGCGGGATTGTGCATCGGGACCTTAAGCCGGAAAATGTTCTCATGGATGCTGATGGGCATGTGATGCTGACGGATTTTGGACTAGCAAAGGAAATTGATGAAGATAGCAGATCGAATTCAATGTGTGGAACCACCGAATACATGGCTCCTGAAATTCTGCTGTCTAAAGGCCACAACAAAGATGCAGATTGGTGGAGCATTGGTATACTTTTGTATGAGATGCTAAGTGGTCAGCCACCATTTACGCATGCAAATAGAAAGAAACTTCAGGAGAGAATCATCAAAGAGAAAGTTAAACTTCCACCTTACCTTACCACTGAAGCTCACTCTTTGCTCAAGGGATTGCTACAAAAGGAACCGTCCCAAAGGTTAGGCAGTGGCCCCAGTGGAGGTGATAATATCAAAAGTCATAAATGGTTTAAAACAATCAACTGGAAGAAGTTGGAGGCCAGAGAACTGCTCCCAAAGTTCAAGCCAGATGTGACTGGAAAAGAATGCACGGCCAATTTTGACCGGTGCTGGACAACAATGCCTCTAGATGACTCACCAGCTCCTACACCAACAGCGGGTGAGCATTTTCAGGGATATACTTACGTGGCACCTAACACTTGGCTTTCATCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

454

Amino Acids

50.8

Weight (kDa)

6.47

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 124 - 386 1.2e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 125 - 369 1.6e-39 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 204 - 285 2.7e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 228 - 368 9.8e-10 Kinase-like
Pkinase_fungal PF17667 237 - 309 2.1e-08 Fungal protein kinase
Pkinase_C PF00433 407 - 444 1.8e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 1336
AccBSI CCGCTC 1 cut(s) 204
AccI GTMKAC 1 cut(s) 943
AccII CGCG 3 cut(s) 161, 232, 401
AclWI GGATC 2 cut(s) 67, 690
AcoI YGGCCR 1 cut(s) 1239
AcsI RAATTY 2 cut(s) 853, 891
AcuI CTGAAG 2 cut(s) 989, 1077
AcyI GRCGYC 2 cut(s) 123, 309
AfaI GTAC 2 cut(s) 611, 659
AfiI CCNNNNNNNGG 3 cut(s) 766, 1127, 1304
AflII CTTAAG 1 cut(s) 761
AgeI ACCGGT 1 cut(s) 1251
AgsI TTSAA 2 cut(s) 858, 1210
AluBI AGCT 5 cut(s) 67, 577, 607, 1061, 1289
AluI AGCT 5 cut(s) 67, 577, 607, 1061, 1289
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 1 cut(s) 485
Alw44I GTGCAC 1 cut(s) 557
AlwI GGATC 2 cut(s) 67, 690
AoxI GGCC 7 cut(s) 130, 220, 359, 907, 1117, 1185, 1239
ApaLI GTGCAC 1 cut(s) 557
ApoI RAATTY 2 cut(s) 853, 891
AsiGI ACCGGT 1 cut(s) 1251
Asp700I GAANNNNTTC 1 cut(s) 774
AspLEI GCGC 3 cut(s) 161, 234, 401
AspS9I GGNCC 5 cut(s) 131, 221, 359, 757, 1118
AsuHPI GGTGA 8 cut(s) 52, 262, 433, 467, 538, 1142, 1275, 1319
AvaII GGWCC 1 cut(s) 757
BaeGI GKGCMC 1 cut(s) 561
BanI GGYRCC 1 cut(s) 1336
BbsI GAAGAC 2 cut(s) 118, 303
Bbv12I GWGCWC 1 cut(s) 561
BccI CCATC 4 cut(s) 434, 446, 452, 785
BceAI ACGGC 4 cut(s) 163, 169, 296, 1254
BclI TGATCA 1 cut(s) 615
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 2 cut(s) 821, 1274
BfrI CTTAAG 1 cut(s) 761
BisI GCNGC 2 cut(s) 157, 276
BlsI GCNGC 2 cut(s) 158, 277
Bme18I GGWCC 1 cut(s) 757
BmgT120I GGNCC 5 cut(s) 131, 221, 359, 757, 1118
BmiI GGNNCC 8 cut(s) 133, 223, 758, 868, 885, 1095, 1120, 1338
BmrI ACTGGG 1 cut(s) 1116
BmsI GCATC 5 cut(s) 760, 775, 792, 910, 948
BmuI ACTGGG 1 cut(s) 1116
BpiI GAAGAC 2 cut(s) 118, 303
BplI GAGNNNNNCTC 2 cut(s) 1182, 1214
BpuEI CTTGAG 2 cut(s) 87, 1058
BsaAI YACGTR 1 cut(s) 1333
BsaHI GRCGYC 2 cut(s) 123, 309
BsaJI CCNNGG 2 cut(s) 291, 362
BsaWI WCCGGW 1 cut(s) 1251
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 925, 955
Bsc4I CCNNNNNNNGG 3 cut(s) 766, 1127, 1304
Bse118I RCCGGY 2 cut(s) 660, 1251
Bse1I ACTGG 3 cut(s) 1122, 1175, 1228
BseDI CCNNGG 2 cut(s) 291, 362
BseGI GGATG 2 cut(s) 192, 790
BseLI CCNNNNNNNGG 3 cut(s) 766, 1127, 1304
BseMII CTCAG 4 cut(s) 29, 394, 522, 696
BseNI ACTGG 3 cut(s) 1122, 1175, 1228
BseRI GAGGAG 3 cut(s) 76, 244, 251
BseSI GKGCMC 1 cut(s) 561
Bsh1236I CGCG 3 cut(s) 161, 232, 401
BshFI GGCC 7 cut(s) 132, 222, 361, 909, 1119, 1187, 1241
BshNI GGYRCC 1 cut(s) 1336
BshTI ACCGGT 1 cut(s) 1251
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 3 cut(s) 661, 767, 1252
BslFI GGGAC 3 cut(s) 551, 770, 1084
BslI CCNNNNNNNGG 3 cut(s) 766, 1127, 1304
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 3 cut(s) 551, 770, 1084
BsmI GAATGC 1 cut(s) 1238
BsnI GGCC 7 cut(s) 132, 222, 361, 909, 1119, 1187, 1241
Bsp1286I GDGCHC 1 cut(s) 561
Bsp143I GATC 4 cut(s) 72, 615, 682, 848
BspANI GGCC 7 cut(s) 132, 222, 361, 909, 1119, 1187, 1241
BspCNI CTCAG 4 cut(s) 28, 393, 523, 697
BspFNI CGCG 3 cut(s) 161, 232, 401
BspHI TCATGA 2 cut(s) 480, 627
BspLI GGNNCC 8 cut(s) 133, 223, 758, 868, 885, 1095, 1120, 1338
BspPI GGATC 2 cut(s) 67, 690
BspQI GCTCTTC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 1336
BspTI CTTAAG 1 cut(s) 761
BsrBI CCGCTC 1 cut(s) 204
BsrFI RCCGGY 2 cut(s) 660, 1251
BsrI ACTGG 3 cut(s) 1122, 1175, 1228
BssAI RCCGGY 2 cut(s) 660, 1251
BssECI CCNNGG 2 cut(s) 291, 362
BssMI GATC 4 cut(s) 72, 615, 682, 848
BssNAI GTATAC 1 cut(s) 944
BssNI GRCGYC 2 cut(s) 123, 309
BssT1I CCWWGG 1 cut(s) 362
Bst1107I GTATAC 1 cut(s) 944
Bst4CI ACNGT 3 cut(s) 325, 496, 1098
Bst6I CTCTTC 2 cut(s) 94, 425
BstACI GRCGYC 2 cut(s) 123, 309
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 802
BstBAI YACGTR 1 cut(s) 1333
BstC8I GCNNGC 1 cut(s) 987
BstDEI CTNAG 5 cut(s) 15, 380, 531, 705, 962
BstF5I GGATG 2 cut(s) 192, 790
BstFNI CGCG 3 cut(s) 161, 232, 401
BstHHI GCGC 3 cut(s) 161, 234, 401
BstKTI GATC 4 cut(s) 75, 618, 685, 851
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 4 cut(s) 72, 615, 682, 848
BstMWI GCNNNNNNNGC 2 cut(s) 441, 802
BstNSI RCATGY 2 cut(s) 800, 989
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 3 cut(s) 161, 232, 401
BstV2I GAAGAC 2 cut(s) 118, 303
BstZ17I GTATAC 1 cut(s) 944
BsuRI GGCC 7 cut(s) 132, 222, 361, 909, 1119, 1187, 1241
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 2 cut(s) 192, 790
BtsI GCAGTG 3 cut(s) 442, 699, 1120
BtsIMutI CAGTG 6 cut(s) 442, 682, 699, 1053, 1120, 1129
Cac8I GCNNGC 1 cut(s) 987
CciI TCATGA 2 cut(s) 480, 627
CfoI GCGC 3 cut(s) 161, 234, 401
Cfr10I RCCGGY 2 cut(s) 660, 1251
Cfr13I GGNCC 5 cut(s) 131, 221, 359, 757, 1118
CseI GACGC 2 cut(s) 150, 317
Csp6I GTAC 2 cut(s) 610, 658
CspAI ACCGGT 1 cut(s) 1251
CviQI GTAC 2 cut(s) 610, 658
DdeI CTNAG 5 cut(s) 15, 380, 531, 705, 962
DpnI GATC 4 cut(s) 74, 617, 684, 850
DpnII GATC 4 cut(s) 72, 615, 682, 848
DraI TTTAAA 1 cut(s) 1159
EaeI YGGCCR 1 cut(s) 1239
Eam1104I CTCTTC 2 cut(s) 94, 425
EarI CTCTTC 2 cut(s) 94, 425
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 757
Eco57I CTGAAG 2 cut(s) 989, 1077
EcoO109I RGGNCCY 1 cut(s) 757
EcoRI GAATTC 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FalI AAGNNNNNCTT 4 cut(s) 596, 628, 1020, 1052
FaqI GGGAC 3 cut(s) 551, 770, 1084
FauI CCCGC 3 cut(s) 237, 734, 1297
FbaI TGATCA 1 cut(s) 615
FblI GTMKAC 1 cut(s) 943
Fnu4HI GCNGC 2 cut(s) 157, 276
FokI GGATG 2 cut(s) 179, 797
Fsp4HI GCNGC 2 cut(s) 157, 276
FspBI CTAG 2 cut(s) 821, 1274
GlaI GCGC 3 cut(s) 160, 233, 400
GluI GCNGC 2 cut(s) 157, 276
HaeIII GGCC 7 cut(s) 132, 222, 361, 909, 1119, 1187, 1241
HapII CCGG 3 cut(s) 661, 767, 1252
HgaI GACGC 2 cut(s) 150, 317
HhaI GCGC 3 cut(s) 161, 234, 401
Hin1I GRCGYC 2 cut(s) 123, 309
Hin6I GCGC 3 cut(s) 159, 232, 399
HinP1I GCGC 3 cut(s) 159, 232, 399
HinfI GANTC 7 cut(s) 281, 287, 338, 376, 384, 1014, 1280
HpaII CCGG 3 cut(s) 661, 767, 1252
HphI GGTGA 8 cut(s) 52, 262, 433, 467, 538, 1142, 1275, 1319
Hpy166II GTNNAC 3 cut(s) 165, 559, 944
Hpy188I TCNGA 7 cut(s) 18, 286, 292, 319, 351, 383, 594
Hpy8I GTNNAC 3 cut(s) 165, 559, 944
Hpy99I CGWCG 6 cut(s) 125, 155, 184, 221, 311, 392
HpyAV CCTTC 7 cut(s) 90, 223, 277, 403, 469, 481, 520
HpyCH4III ACNGT 3 cut(s) 325, 496, 1098
HpyCH4IV ACGT 2 cut(s) 123, 1332
HpyCH4V TGCA 5 cut(s) 559, 751, 923, 989, 1236
HpyF10VI GCNNNNNNNGC 2 cut(s) 441, 802
HpyF3I CTNAG 5 cut(s) 15, 380, 531, 705, 962
HpySE526I ACGT 2 cut(s) 123, 1332
Hsp92I GRCGYC 2 cut(s) 123, 309
HspAI GCGC 3 cut(s) 159, 232, 399
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 4 cut(s) 72, 615, 682, 848
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 6 cut(s) 209, 582, 889, 933, 1203, 1294
LweI GCATC 5 cut(s) 760, 775, 792, 910, 948
MaeI CTAG 2 cut(s) 821, 1274
MaeII ACGT 2 cut(s) 123, 1332
MaeIII GTNAC 2 cut(s) 183, 1219
MalI GATC 4 cut(s) 74, 617, 684, 850
MbiI CCGCTC 1 cut(s) 204
MboI GATC 4 cut(s) 72, 615, 682, 848
MhlI GDGCHC 1 cut(s) 561
MluCI AATT 4 cut(s) 831, 853, 891, 1243
MlyI GAGTC 3 cut(s) 281, 393, 1274
MmeI TCCRAC 3 cut(s) 171, 309, 1161
MroXI GAANNNNTTC 1 cut(s) 774
MseI TTAA 4 cut(s) 117, 762, 1032, 1158
MspA1I CMGCKG 1 cut(s) 1304
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 3 cut(s) 661, 767, 1252
Mva1269I GAATGC 1 cut(s) 1238
MvnI CGCG 3 cut(s) 161, 232, 401
MwoI GCNNNNNNNGC 2 cut(s) 441, 802
NdeII GATC 4 cut(s) 72, 615, 682, 848
NlaIV GGNNCC 8 cut(s) 133, 223, 758, 868, 885, 1095, 1120, 1338
NmuCI GTSAC 1 cut(s) 1219
NspI RCATGY 2 cut(s) 800, 989
PaeI GCATGC 1 cut(s) 989
PagI TCATGA 2 cut(s) 480, 627
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 315
PctI GAATGC 1 cut(s) 1238
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 4 cut(s) 281, 338, 376, 1014
PinAI ACCGGT 1 cut(s) 1251
PkrI GCNGC 2 cut(s) 158, 277
PleI GAGTC 3 cut(s) 281, 392, 1274
PpsI GAGTC 3 cut(s) 281, 392, 1274
Ppu21I YACGTR 1 cut(s) 1333
PpuMI RGGWCCY 1 cut(s) 757
Psp5II RGGWCCY 1 cut(s) 757
PspN4I GGNNCC 8 cut(s) 133, 223, 758, 868, 885, 1095, 1120, 1338
PspPI GGNCC 5 cut(s) 131, 221, 359, 757, 1118
PspPPI RGGWCCY 1 cut(s) 757
RsaI GTAC 2 cut(s) 611, 659
RsaNI GTAC 2 cut(s) 610, 658
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 4 cut(s) 117, 762, 1032, 1158
SatI GCNGC 2 cut(s) 157, 276
Sau3AI GATC 4 cut(s) 72, 615, 682, 848
Sau96I GGNCC 5 cut(s) 131, 221, 359, 757, 1118
SchI GAGTC 3 cut(s) 281, 393, 1274
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 5 cut(s) 760, 775, 792, 910, 948
SinI GGWCC 1 cut(s) 757
SmlI CTYRAG 3 cut(s) 102, 761, 1073
SmoI CTYRAG 3 cut(s) 102, 761, 1073
SphI GCATGC 1 cut(s) 989
Sse9I AATT 4 cut(s) 831, 853, 891, 1243
SspMI CTAG 2 cut(s) 821, 1274
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 3 cut(s) 325, 496, 1098
TaiI ACGT 2 cut(s) 126, 1335
TaqI TCGA 3 cut(s) 111, 372, 851
TasI AATT 4 cut(s) 831, 853, 891, 1243
TatI WGTACW 1 cut(s) 609
TauI GCSGC 2 cut(s) 159, 278
TfiI GAWTC 4 cut(s) 281, 338, 376, 1014
Tru1I TTAA 4 cut(s) 117, 762, 1032, 1158
Tru9I TTAA 4 cut(s) 117, 762, 1032, 1158
TscAI CASTG 6 cut(s) 442, 682, 706, 1060, 1120, 1129
TseFI GTSAC 1 cut(s) 1219
Tsp45I GTSAC 1 cut(s) 1219
TspDTI ATGAA 8 cut(s) 159, 462, 488, 539, 616, 644, 852, 1344
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 6 cut(s) 442, 682, 706, 1060, 1120, 1129
Vha464I CTTAAG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 557
VpaK11BI GGWCC 1 cut(s) 757
XapI RAATTY 2 cut(s) 853, 891
XbaI TCTAGA 1 cut(s) 1273
XceI RCATGY 2 cut(s) 800, 989
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XmiI GTMKAC 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 774
XspI CTAG 2 cut(s) 821, 1274
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.