RLG00000005301

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007510
Physical Location & Seq
Forward (+)
66626169 .. 66642489
16321 bp
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UTR
Exon/CDS
Intron
RLM00000005301

Sequence Viewer

Length: 1362 bp
ATGGTGTCCTCCTCTCAGAACAATAACAAAACCCTCCACTCCCTCCTTGCCACAAAGCTCACCAAGCTCACAATCCCACCCTCCTCCTCCTCCTCCTCTTCTCCGCCTCAAGACTTCGACTTTAACGACGTCTTCGGCCCCCCAACCGCCTCCAACGACGGCGGCGCGTCCACGTCATACTCGCCGTCGTTACTTGTCATCCACAACCGCTCCCACTCCTTCGTCGGCCCCTCCCCGCGCTTCACTCCCTCCTCCCTCCTCCCCTTCACCGCCGCCGAATCCGACTCCGAGGAAGACGAAAGCGACGCCGTTTCCGAAACCCTGAAAGATGAGGAATCGGCGGTTCGGAAGTTCGGGCCTTCGGATTTTGAGATTCTCAGAGTCGTCGGCAAGGGCGCGTTCGGGAAGGTGTTTCAGGTGAGGAAGAAGAGCAGTGATGGCGAGGAAGATGGTGATGGGCTTTATGCCATGAAGGTCATGAGGAAGGAGACTGTTATCAAGAAGAACCATGTGGATTACATGAAGGCTGAGAGGGACATTCTCACCAAAGTTGTGCACCCTTTTATTGTTCGGCTCCGCTACTCTTTTCAGACCAAGTGTAAGCTGTACTTGATCATGGATTTCATAAATGGAGGGCATTTGTTCTATCATTTGTACCGGCAGGGGATATTCAGTGAGGATCAGGCGAGGTTTTATACTGCTCAGATAGTGTCTGCTGTTTCACATCTTCACAAGTGCGGGATTGTGCACCGGGACCTTAAGCCGGAAAATGTGCTCATGGATGCTGATGGGCATGTGATGCTGACGGATTTTGGACTAGCAAAGGAAATTGACGAAGATAGCAGATCGAATTCAATGTGTGGAACCACCGAATACATGGCTCCTGAAATTCTGCTGTCTAAAGGCCACAACAAAGATGCAGATTGGTGGAGCATTGGTATACTTTTGTATGAGATGCTAAGTGGTCAGCCACCATTTACGCATGCAAATAGAAAGAAACTTCAGGAGAGAATCATCAGAGAGAAAGTTAAACTTCCACCTTACCTTACCACTGAAGCTCACTCTTTGCTCAAAGGATTGTTACAAAAGGAACCATCCCAAGGGTTAGGCAGTGGCCCCAGTGGAGGTGATAATATCAAAAGTCATAAATGGTTTAAAACAATCAACTGGAAGAAGTTGGAGGCCAGAGAACTGCTCCCAAAGTTCAAGCCAGATGTGACTGGAAAAGAATGCACGGCAAATTTTGACCGGTGCTGGACAACAATGCCTCTAGATGATTCACCAGCTCCTACACCAACAGCGGGTGAGCATTTTCAGGGATATACTTATGTGGCACCTAACACTTGGCTTTCATCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

454

Amino Acids

50.59

Weight (kDa)

6.77

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 123 - 385 9.7e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 124 - 366 1.9e-38 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 203 - 284 2.2e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 227 - 363 1.4e-10 Kinase-like
Pkinase_fungal PF17667 237 - 308 2.3e-08 Fungal protein kinase
Pkinase_C PF00433 406 - 443 1.7e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 1333
AccBSI CCGCTC 1 cut(s) 210
AccI GTMKAC 1 cut(s) 940
AccII CGCG 3 cut(s) 167, 238, 398
AclWI GGATC 1 cut(s) 687
AcsI RAATTY 3 cut(s) 850, 888, 1240
AcuI CTGAAG 2 cut(s) 986, 1074
AcyI GRCGYC 2 cut(s) 129, 306
AfaI GTAC 2 cut(s) 608, 656
AfiI CCNNNNNNNGG 4 cut(s) 763, 1100, 1124, 1301
AflII CTTAAG 1 cut(s) 758
AgeI ACCGGT 1 cut(s) 1248
AgsI TTSAA 2 cut(s) 855, 1207
AjiI CACGTC 1 cut(s) 174
AluBI AGCT 5 cut(s) 58, 67, 604, 1058, 1286
AluI AGCT 5 cut(s) 58, 67, 604, 1058, 1286
Alw21I GWGCWC 3 cut(s) 558, 750, 777
Alw26I GTCTC 1 cut(s) 482
Alw44I GTGCAC 2 cut(s) 554, 746
AlwI GGATC 1 cut(s) 687
AoxI GGCC 6 cut(s) 136, 226, 356, 904, 1114, 1182
ApaLI GTGCAC 2 cut(s) 554, 746
ApoI RAATTY 3 cut(s) 850, 888, 1240
AsiGI ACCGGT 1 cut(s) 1248
AspLEI GCGC 3 cut(s) 167, 240, 398
AspS9I GGNCC 5 cut(s) 137, 227, 356, 754, 1115
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 8 cut(s) 52, 259, 430, 464, 535, 1139, 1272, 1316
AvaII GGWCC 1 cut(s) 754
BaeGI GKGCMC 2 cut(s) 558, 750
BanI GGYRCC 1 cut(s) 1333
BbsI GAAGAC 2 cut(s) 124, 300
Bbv12I GWGCWC 3 cut(s) 558, 750, 777
BccI CCATC 5 cut(s) 431, 443, 449, 782, 1102
BceAI ACGGC 4 cut(s) 169, 175, 293, 1251
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 1 cut(s) 752
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 2 cut(s) 818, 1271
BfrI CTTAAG 1 cut(s) 758
BisI GCNGC 2 cut(s) 163, 273
BlsI GCNGC 2 cut(s) 164, 274
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 1 cut(s) 754
BmgBI CACGTC 1 cut(s) 174
BmgT120I GGNCC 5 cut(s) 137, 227, 356, 754, 1115
BmiI GGNNCC 9 cut(s) 139, 229, 575, 755, 865, 882, 1092, 1117, 1335
BmrFI CCNGG 1 cut(s) 752
BmrI ACTGGG 1 cut(s) 1113
BmsI GCATC 4 cut(s) 772, 789, 907, 945
BmuI ACTGGG 1 cut(s) 1113
BpiI GAAGAC 2 cut(s) 124, 300
BplI GAGNNNNNCTC 2 cut(s) 1179, 1211
BpuEI CTTGAG 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 752
BsaHI GRCGYC 2 cut(s) 129, 306
BsaJI CCNNGG 2 cut(s) 288, 1099
BsaWI WCCGGW 1 cut(s) 1248
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 922, 952
Bsc4I CCNNNNNNNGG 4 cut(s) 763, 1100, 1124, 1301
Bse118I RCCGGY 2 cut(s) 657, 1248
Bse1I ACTGG 3 cut(s) 1119, 1172, 1225
BseDI CCNNGG 2 cut(s) 288, 1099
BseGI GGATG 3 cut(s) 198, 787, 1094
BseLI CCNNNNNNNGG 4 cut(s) 763, 1100, 1124, 1301
BseMII CTCAG 4 cut(s) 29, 391, 519, 716
BseNI ACTGG 3 cut(s) 1119, 1172, 1225
BseRI GAGGAG 7 cut(s) 73, 76, 79, 82, 85, 241, 248
BseSI GKGCMC 2 cut(s) 558, 750
Bsh1236I CGCG 3 cut(s) 167, 238, 398
BshFI GGCC 6 cut(s) 138, 228, 358, 906, 1116, 1184
BshNI GGYRCC 1 cut(s) 1333
BshTI ACCGGT 1 cut(s) 1248
BsiHKAI GWGCWC 3 cut(s) 558, 750, 777
BsiSI CCGG 4 cut(s) 658, 751, 764, 1249
BslFI GGGAC 2 cut(s) 548, 767
BslI CCNNNNNNNGG 4 cut(s) 763, 1100, 1124, 1301
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 2 cut(s) 548, 767
BsmI GAATGC 1 cut(s) 1235
BsnI GGCC 6 cut(s) 138, 228, 358, 906, 1116, 1184
Bsp1286I GDGCHC 3 cut(s) 558, 750, 777
Bsp143I GATC 3 cut(s) 612, 679, 845
BspANI GGCC 6 cut(s) 138, 228, 358, 906, 1116, 1184
BspCNI CTCAG 4 cut(s) 28, 390, 520, 715
BspFNI CGCG 3 cut(s) 167, 238, 398
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 9 cut(s) 139, 229, 575, 755, 865, 882, 1092, 1117, 1335
BspPI GGATC 1 cut(s) 687
BspQI GCTCTTC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 1333
BspTI CTTAAG 1 cut(s) 758
BsrBI CCGCTC 1 cut(s) 210
BsrFI RCCGGY 2 cut(s) 657, 1248
BsrI ACTGG 3 cut(s) 1119, 1172, 1225
BssAI RCCGGY 2 cut(s) 657, 1248
BssECI CCNNGG 2 cut(s) 288, 1099
BssMI GATC 3 cut(s) 612, 679, 845
BssNAI GTATAC 1 cut(s) 941
BssNI GRCGYC 2 cut(s) 129, 306
BssT1I CCWWGG 1 cut(s) 1099
Bst1107I GTATAC 1 cut(s) 941
Bst4CI ACNGT 1 cut(s) 493
Bst6I CTCTTC 2 cut(s) 103, 422
BstACI GRCGYC 2 cut(s) 129, 306
BstAFI CTTAAG 1 cut(s) 758
BstAPI GCANNNNNTGC 1 cut(s) 799
BstC8I GCNNGC 1 cut(s) 984
BstDEI CTNAG 5 cut(s) 15, 377, 528, 702, 959
BstF5I GGATG 3 cut(s) 198, 787, 1094
BstFNI CGCG 3 cut(s) 167, 238, 398
BstHHI GCGC 3 cut(s) 167, 240, 398
BstKTI GATC 3 cut(s) 615, 682, 848
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 3 cut(s) 612, 679, 845
BstMWI GCNNNNNNNGC 3 cut(s) 64, 438, 799
BstNSI RCATGY 2 cut(s) 797, 986
BstSCI CCNGG 1 cut(s) 750
BstSLI GKGCMC 2 cut(s) 558, 750
BstUI CGCG 3 cut(s) 167, 238, 398
BstV2I GAAGAC 2 cut(s) 124, 300
BstZ17I GTATAC 1 cut(s) 941
BsuRI GGCC 6 cut(s) 138, 228, 358, 906, 1116, 1184
BtrI CACGTC 1 cut(s) 174
BtsCI GGATG 3 cut(s) 198, 787, 1094
BtsI GCAGTG 2 cut(s) 439, 1117
BtsIMutI CAGTG 5 cut(s) 439, 679, 1050, 1117, 1126
Cac8I GCNNGC 1 cut(s) 984
CciI TCATGA 1 cut(s) 477
CfoI GCGC 3 cut(s) 167, 240, 398
Cfr10I RCCGGY 2 cut(s) 657, 1248
Cfr13I GGNCC 5 cut(s) 137, 227, 356, 754, 1115
CseI GACGC 2 cut(s) 156, 314
Csp6I GTAC 2 cut(s) 607, 655
CspAI ACCGGT 1 cut(s) 1248
CviAII CATG 9 cut(s) 469, 478, 509, 520, 616, 778, 794, 877, 983
CviQI GTAC 2 cut(s) 607, 655
DdeI CTNAG 5 cut(s) 15, 377, 528, 702, 959
DpnI GATC 3 cut(s) 614, 681, 847
DpnII GATC 3 cut(s) 612, 679, 845
DraI TTTAAA 1 cut(s) 1156
Eam1104I CTCTTC 2 cut(s) 103, 422
EarI CTCTTC 2 cut(s) 103, 422
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 1099
Eco47I GGWCC 1 cut(s) 754
Eco57I CTGAAG 2 cut(s) 986, 1074
EcoO109I RGGNCCY 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 850
EcoT14I CCWWGG 1 cut(s) 1099
ErhI CCWWGG 1 cut(s) 1099
FaeI CATG 9 cut(s) 472, 481, 512, 523, 619, 781, 797, 880, 986
FalI AAGNNNNNCTT 4 cut(s) 593, 625, 1017, 1049
FaqI GGGAC 2 cut(s) 548, 767
FatI CATG 9 cut(s) 468, 477, 508, 519, 615, 777, 793, 876, 982
FauI CCCGC 3 cut(s) 243, 731, 1294
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 1 cut(s) 940
Fnu4HI GCNGC 2 cut(s) 163, 273
FokI GGATG 3 cut(s) 185, 794, 1081
Fsp4HI GCNGC 2 cut(s) 163, 273
FspBI CTAG 2 cut(s) 818, 1271
GlaI GCGC 3 cut(s) 166, 239, 397
GluI GCNGC 2 cut(s) 163, 273
HaeIII GGCC 6 cut(s) 138, 228, 358, 906, 1116, 1184
HapII CCGG 4 cut(s) 658, 751, 764, 1249
HgaI GACGC 2 cut(s) 156, 314
HhaI GCGC 3 cut(s) 167, 240, 398
Hin1I GRCGYC 2 cut(s) 129, 306
Hin1II CATG 9 cut(s) 472, 481, 512, 523, 619, 781, 797, 880, 986
Hin6I GCGC 3 cut(s) 165, 238, 396
HinP1I GCGC 3 cut(s) 165, 238, 396
HinfI GANTC 7 cut(s) 278, 284, 335, 373, 381, 1011, 1277
HpaII CCGG 4 cut(s) 658, 751, 764, 1249
HphI GGTGA 8 cut(s) 52, 259, 430, 464, 535, 1139, 1272, 1316
Hpy166II GTNNAC 4 cut(s) 171, 556, 748, 941
Hpy188III TCNNGA 7 cut(s) 110, 403, 478, 499, 884, 1004, 1271
Hpy8I GTNNAC 4 cut(s) 171, 556, 748, 941
Hpy99I CGWCG 6 cut(s) 131, 161, 190, 227, 308, 389
HpyAV CCTTC 7 cut(s) 229, 274, 369, 400, 466, 478, 517
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4IV ACGT 2 cut(s) 129, 173
HpyCH4V TGCA 5 cut(s) 556, 748, 920, 986, 1233
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 438, 799
HpyF3I CTNAG 5 cut(s) 15, 377, 528, 702, 959
HpySE526I ACGT 2 cut(s) 129, 173
Hsp92I GRCGYC 2 cut(s) 129, 306
Hsp92II CATG 9 cut(s) 472, 481, 512, 523, 619, 781, 797, 880, 986
HspAI GCGC 3 cut(s) 165, 238, 396
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 3 cut(s) 612, 679, 845
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 6 cut(s) 215, 579, 886, 930, 1200, 1291
LweI GCATC 4 cut(s) 772, 789, 907, 945
MaeI CTAG 2 cut(s) 818, 1271
MaeII ACGT 2 cut(s) 129, 173
MaeIII GTNAC 3 cut(s) 189, 1080, 1216
MalI GATC 3 cut(s) 614, 681, 847
MbiI CCGCTC 1 cut(s) 210
MboI GATC 3 cut(s) 612, 679, 845
MhlI GDGCHC 3 cut(s) 558, 750, 777
MluCI AATT 4 cut(s) 828, 850, 888, 1240
MlyI GAGTC 2 cut(s) 278, 390
MmeI TCCRAC 3 cut(s) 177, 306, 1158
MseI TTAA 4 cut(s) 123, 759, 1029, 1155
MspA1I CMGCKG 1 cut(s) 1301
MspCI CTTAAG 1 cut(s) 758
MspI CCGG 4 cut(s) 658, 751, 764, 1249
MspR9I CCNGG 1 cut(s) 752
Mva1269I GAATGC 1 cut(s) 1235
MvnI CGCG 3 cut(s) 167, 238, 398
MwoI GCNNNNNNNGC 3 cut(s) 64, 438, 799
NciI CCSGG 1 cut(s) 752
NdeII GATC 3 cut(s) 612, 679, 845
NlaIII CATG 9 cut(s) 472, 481, 512, 523, 619, 781, 797, 880, 986
NlaIV GGNNCC 9 cut(s) 139, 229, 575, 755, 865, 882, 1092, 1117, 1335
NmuCI GTSAC 1 cut(s) 1216
NspI RCATGY 2 cut(s) 797, 986
PaeI GCATGC 1 cut(s) 986
PagI TCATGA 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 312
PctI GAATGC 1 cut(s) 1235
PfeI GAWTC 5 cut(s) 278, 335, 373, 1011, 1277
PinAI ACCGGT 1 cut(s) 1248
PkrI GCNGC 2 cut(s) 164, 274
PleI GAGTC 2 cut(s) 278, 389
PpsI GAGTC 2 cut(s) 278, 389
PpuMI RGGWCCY 1 cut(s) 754
Psp5II RGGWCCY 1 cut(s) 754
PspN4I GGNNCC 9 cut(s) 139, 229, 575, 755, 865, 882, 1092, 1117, 1335
PspPI GGNCC 5 cut(s) 137, 227, 356, 754, 1115
PspPPI RGGWCCY 1 cut(s) 754
RsaI GTAC 2 cut(s) 608, 656
RsaNI GTAC 2 cut(s) 607, 655
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 4 cut(s) 123, 759, 1029, 1155
SatI GCNGC 2 cut(s) 163, 273
Sau3AI GATC 3 cut(s) 612, 679, 845
Sau96I GGNCC 5 cut(s) 137, 227, 356, 754, 1115
SchI GAGTC 2 cut(s) 278, 390
ScrFI CCNGG 1 cut(s) 752
SduI GDGCHC 3 cut(s) 558, 750, 777
SfaNI GCATC 4 cut(s) 772, 789, 907, 945
SinI GGWCC 1 cut(s) 754
SmlI CTYRAG 2 cut(s) 108, 758
SmoI CTYRAG 2 cut(s) 108, 758
SphI GCATGC 1 cut(s) 986
Sse9I AATT 4 cut(s) 828, 850, 888, 1240
SspMI CTAG 2 cut(s) 818, 1271
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 1 cut(s) 1099
TaaI ACNGT 1 cut(s) 493
TaiI ACGT 2 cut(s) 132, 176
TaqI TCGA 2 cut(s) 117, 848
TasI AATT 4 cut(s) 828, 850, 888, 1240
TatI WGTACW 1 cut(s) 606
TauI GCSGC 2 cut(s) 165, 275
TfiI GAWTC 5 cut(s) 278, 335, 373, 1011, 1277
Tru1I TTAA 4 cut(s) 123, 759, 1029, 1155
Tru9I TTAA 4 cut(s) 123, 759, 1029, 1155
TscAI CASTG 5 cut(s) 439, 679, 1057, 1117, 1126
TseFI GTSAC 1 cut(s) 1216
Tsp45I GTSAC 1 cut(s) 1216
TspDTI ATGAA 4 cut(s) 485, 536, 613, 1341
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 5 cut(s) 439, 679, 1057, 1117, 1126
Vha464I CTTAAG 1 cut(s) 758
VneI GTGCAC 2 cut(s) 554, 746
VpaK11BI GGWCC 1 cut(s) 754
XapI RAATTY 3 cut(s) 850, 888, 1240
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 2 cut(s) 797, 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XmiI GTMKAC 1 cut(s) 940
XspI CTAG 2 cut(s) 818, 1271
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.