Rw7G002440

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007510
Physical Location & Seq
Reverse (-)
1995071 .. 1997370
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002440.1

Sequence Viewer

Length: 1356 bp
ATGGTGTCCTCCTCTCAGAACAATAACAAAACCCTCCATTCCCTTCTTGCCTCAAACCTCACCAAGCTCACAATCCCACCTTCCTCCTCTTCTTCTCCGCCTCAAGACTTCGACTTTAACGACGTCTTCGGCCCCCCAACCGCCTCCAACGACGGCGGCGCGTCCACTTCATTCTCGCCGTCGTTACTCGTCATCCACAACCGCTCCCACTCCTTCGTCGGCCCCTCCCCGCGCTTCACTCCCTCCTCCCTCCTCCCCTTCACCGCCGCCGACTCCGACTCCGAGGAAGACGAAAACGACGCCGTTTCCGAAACCGTGAAAGATGAGGAATCGGCGGTTCGGAAGTTCGGGCCTTGGGATTTCGAGATTCTCAGAGTCGTCGGCAAGGGCGCGTTCGGGAAGGTGTTTCAGGTGAGGAAGAAGAGCAGTGATGGCGATGAAGATGGTGATGGGCTTTATGCCATGAAGGTCATGAGGAAGGAGACTGTTATCAAGAAGAACCATGTGGATTACATGAAGGCTGAGAGGGACATTCTCACCAAAGTTGTGCACCCTTTTATTGTTCAGCTCCGCTACTCTTTTCAGACCAAGTGTAAGCTGTACTTGATCATGGATTTCATGAATGGAGGGCATTTGTTCTATCATTTGTACCGGCAGGGGATATTCAGTGAGGATCAGGCGAGGTTTTACACTGCTGAGATAGTGTCTGCTGTTTCACATCTTCACAAATGCGGGATTGTGCATCGGGACCTTAAGCCGGAAAATGTTCTCATGGATGCTGATGGGCATGTGATGCTGACGGATTTTGGACTAGCAAAGGAAATTGATGAAGATAGCAGATCGAATTCAATGTGTGGAACCACCGAATACATGGCTCCTGAAATTCTGCTGTCTAAAGGCCACAACAAAGATGCAGATTGGTGGAGCATTGGTATACTTTTGTATGAGATGCTAAGTGGTCAGCCGCCATTTACGCATGCAAATAGAAAGAAACTTCAGGAGAGAATCATCAGAGAGAAAGTTAAACTTCCACCTTACCTTACCACTGAAGCTCACTCTTTGCTTAAGGGATTGCTACAAAAGGAACCATCCCAAAGGTTAGGCAGTGGCCCCAGTGGAGGTGATAATATCAAAAGTCATAAATGGTTTAAAACAATCAACTGGAAGAAGTTGGAGGCCAGAGAACTGCTCCCAAAGTTCAAGCCAGATGTGACTGGAAAAGAATGCACGGCCAATTTTGACCGGTGCTGGACAACAATGCCTCTAGATGACTCACCAGCTCCTACACCAACAGCGGGTGAGCATTTTCAGGGATATACTTACGTGGCACCTAACACTTGGCTTTCATCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

451

Amino Acids

50.54

Weight (kDa)

6.47

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 121 - 383 9.9e-74 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 122 - 366 1.9e-39 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 201 - 282 2.7e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 225 - 365 9.6e-10 Kinase-like
Pkinase_fungal PF17667 234 - 306 2.1e-08 Fungal protein kinase
Pkinase_C PF00433 404 - 441 1.7e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 1327
AccBSI CCGCTC 1 cut(s) 204
AccI GTMKAC 1 cut(s) 934
AccII CGCG 3 cut(s) 161, 232, 392
AclWI GGATC 1 cut(s) 681
AcoI YGGCCR 1 cut(s) 1230
AcsI RAATTY 2 cut(s) 844, 882
AcuI CTGAAG 2 cut(s) 980, 1068
AcyI GRCGYC 2 cut(s) 123, 300
AfaI GTAC 2 cut(s) 602, 650
AfiI CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
AflII CTTAAG 2 cut(s) 752, 1064
AgeI ACCGGT 1 cut(s) 1242
AgsI TTSAA 2 cut(s) 849, 1201
AluBI AGCT 5 cut(s) 67, 568, 598, 1052, 1280
AluI AGCT 5 cut(s) 67, 568, 598, 1052, 1280
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 1 cut(s) 476
Alw44I GTGCAC 1 cut(s) 548
AlwI GGATC 1 cut(s) 681
AoxI GGCC 7 cut(s) 130, 220, 350, 898, 1108, 1176, 1230
ApaLI GTGCAC 1 cut(s) 548
ApoI RAATTY 2 cut(s) 844, 882
AsiGI ACCGGT 1 cut(s) 1242
Asp700I GAANNNNTTC 1 cut(s) 765
AspLEI GCGC 3 cut(s) 161, 234, 392
AspS9I GGNCC 5 cut(s) 131, 221, 350, 748, 1109
AsuHPI GGTGA 8 cut(s) 52, 253, 424, 458, 529, 1133, 1266, 1310
AvaII GGWCC 1 cut(s) 748
BaeGI GKGCMC 1 cut(s) 552
BanI GGYRCC 1 cut(s) 1327
BbsI GAAGAC 2 cut(s) 118, 294
Bbv12I GWGCWC 1 cut(s) 552
BccI CCATC 5 cut(s) 425, 437, 443, 776, 1096
BceAI ACGGC 4 cut(s) 163, 169, 287, 1245
BclI TGATCA 1 cut(s) 606
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 2 cut(s) 812, 1265
BfrI CTTAAG 2 cut(s) 752, 1064
BisI GCNGC 3 cut(s) 157, 267, 965
BlsI GCNGC 3 cut(s) 158, 268, 966
Bme18I GGWCC 1 cut(s) 748
BmgT120I GGNCC 5 cut(s) 131, 221, 350, 748, 1109
BmiI GGNNCC 8 cut(s) 133, 223, 749, 859, 876, 1086, 1111, 1329
BmrI ACTGGG 1 cut(s) 1107
BmsI GCATC 5 cut(s) 751, 766, 783, 901, 939
BmuI ACTGGG 1 cut(s) 1107
BpiI GAAGAC 2 cut(s) 118, 294
BplI GAGNNNNNCTC 2 cut(s) 1173, 1205
BpuEI CTTGAG 1 cut(s) 87
BsaAI YACGTR 1 cut(s) 1324
BsaHI GRCGYC 2 cut(s) 123, 300
BsaJI CCNNGG 2 cut(s) 282, 353
BsaWI WCCGGW 1 cut(s) 1242
BsaXI ACNNNNNCTCC 6 cut(s) 188, 218, 263, 293, 916, 946
Bsc4I CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
Bse118I RCCGGY 2 cut(s) 651, 1242
Bse1I ACTGG 3 cut(s) 1113, 1166, 1219
BseDI CCNNGG 2 cut(s) 282, 353
BseGI GGATG 3 cut(s) 192, 781, 1088
BseLI CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
BseMII CTCAG 4 cut(s) 29, 385, 513, 687
BseNI ACTGG 3 cut(s) 1113, 1166, 1219
BseRI GAGGAG 3 cut(s) 76, 235, 242
BseSI GKGCMC 1 cut(s) 552
Bsh1236I CGCG 3 cut(s) 161, 232, 392
BshFI GGCC 7 cut(s) 132, 222, 352, 900, 1110, 1178, 1232
BshNI GGYRCC 1 cut(s) 1327
BshTI ACCGGT 1 cut(s) 1242
BsiHKAI GWGCWC 1 cut(s) 552
BsiSI CCGG 3 cut(s) 652, 758, 1243
BslFI GGGAC 2 cut(s) 542, 761
BslI CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 542, 761
BsmI GAATGC 1 cut(s) 1229
BsnI GGCC 7 cut(s) 132, 222, 352, 900, 1110, 1178, 1232
Bsp1286I GDGCHC 1 cut(s) 552
Bsp143I GATC 3 cut(s) 606, 673, 839
BspANI GGCC 7 cut(s) 132, 222, 352, 900, 1110, 1178, 1232
BspCNI CTCAG 4 cut(s) 28, 384, 514, 688
BspFNI CGCG 3 cut(s) 161, 232, 392
BspHI TCATGA 2 cut(s) 471, 618
BspLI GGNNCC 8 cut(s) 133, 223, 749, 859, 876, 1086, 1111, 1329
BspPI GGATC 1 cut(s) 681
BspQI GCTCTTC 1 cut(s) 416
BspT107I GGYRCC 1 cut(s) 1327
BspTI CTTAAG 2 cut(s) 752, 1064
BsrBI CCGCTC 1 cut(s) 204
BsrFI RCCGGY 2 cut(s) 651, 1242
BsrI ACTGG 3 cut(s) 1113, 1166, 1219
BssAI RCCGGY 2 cut(s) 651, 1242
BssECI CCNNGG 2 cut(s) 282, 353
BssMI GATC 3 cut(s) 606, 673, 839
BssNAI GTATAC 1 cut(s) 935
BssNI GRCGYC 2 cut(s) 123, 300
BssT1I CCWWGG 1 cut(s) 353
Bst1107I GTATAC 1 cut(s) 935
Bst4CI ACNGT 2 cut(s) 316, 487
Bst6I CTCTTC 2 cut(s) 94, 416
BstACI GRCGYC 2 cut(s) 123, 300
BstAFI CTTAAG 2 cut(s) 752, 1064
BstAPI GCANNNNNTGC 1 cut(s) 793
BstBAI YACGTR 1 cut(s) 1324
BstC8I GCNNGC 1 cut(s) 978
BstDEI CTNAG 5 cut(s) 15, 371, 522, 696, 953
BstF5I GGATG 3 cut(s) 192, 781, 1088
BstFNI CGCG 3 cut(s) 161, 232, 392
BstHHI GCGC 3 cut(s) 161, 234, 392
BstKTI GATC 3 cut(s) 609, 676, 842
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 3 cut(s) 606, 673, 839
BstMWI GCNNNNNNNGC 3 cut(s) 432, 793, 973
BstNSI RCATGY 2 cut(s) 791, 980
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 3 cut(s) 161, 232, 392
BstV2I GAAGAC 2 cut(s) 118, 294
BstZ17I GTATAC 1 cut(s) 935
BsuRI GGCC 7 cut(s) 132, 222, 352, 900, 1110, 1178, 1232
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 3 cut(s) 192, 781, 1088
BtsI GCAGTG 3 cut(s) 433, 690, 1111
BtsIMutI CAGTG 6 cut(s) 433, 673, 690, 1044, 1111, 1120
Cac8I GCNNGC 1 cut(s) 978
CciI TCATGA 2 cut(s) 471, 618
CfoI GCGC 3 cut(s) 161, 234, 392
Cfr10I RCCGGY 2 cut(s) 651, 1242
Cfr13I GGNCC 5 cut(s) 131, 221, 350, 748, 1109
CseI GACGC 2 cut(s) 150, 308
Csp6I GTAC 2 cut(s) 601, 649
CspAI ACCGGT 1 cut(s) 1242
CviQI GTAC 2 cut(s) 601, 649
DdeI CTNAG 5 cut(s) 15, 371, 522, 696, 953
DpnI GATC 3 cut(s) 608, 675, 841
DpnII GATC 3 cut(s) 606, 673, 839
DraI TTTAAA 1 cut(s) 1150
EaeI YGGCCR 1 cut(s) 1230
Eam1104I CTCTTC 2 cut(s) 94, 416
EarI CTCTTC 2 cut(s) 94, 416
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 748
Eco57I CTGAAG 2 cut(s) 980, 1068
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 353
FalI AAGNNNNNCTT 4 cut(s) 587, 619, 1011, 1043
FaqI GGGAC 2 cut(s) 542, 761
FauI CCCGC 3 cut(s) 237, 725, 1288
FbaI TGATCA 1 cut(s) 606
FblI GTMKAC 1 cut(s) 934
Fnu4HI GCNGC 3 cut(s) 157, 267, 965
FokI GGATG 3 cut(s) 179, 788, 1075
Fsp4HI GCNGC 3 cut(s) 157, 267, 965
FspBI CTAG 2 cut(s) 812, 1265
GlaI GCGC 3 cut(s) 160, 233, 391
GluI GCNGC 3 cut(s) 157, 267, 965
HaeIII GGCC 7 cut(s) 132, 222, 352, 900, 1110, 1178, 1232
HapII CCGG 3 cut(s) 652, 758, 1243
HgaI GACGC 2 cut(s) 150, 308
HhaI GCGC 3 cut(s) 161, 234, 392
Hin1I GRCGYC 2 cut(s) 123, 300
Hin6I GCGC 3 cut(s) 159, 232, 390
HinP1I GCGC 3 cut(s) 159, 232, 390
HinfI GANTC 7 cut(s) 272, 278, 329, 367, 375, 1005, 1271
HpaII CCGG 3 cut(s) 652, 758, 1243
HphI GGTGA 8 cut(s) 52, 253, 424, 458, 529, 1133, 1266, 1310
Hpy166II GTNNAC 3 cut(s) 165, 550, 935
Hpy188I TCNGA 8 cut(s) 18, 277, 283, 310, 342, 374, 585, 1013
Hpy8I GTNNAC 3 cut(s) 165, 550, 935
Hpy99I CGWCG 6 cut(s) 125, 155, 184, 221, 302, 383
HpyAV CCTTC 8 cut(s) 53, 90, 223, 268, 394, 460, 472, 511
HpyCH4III ACNGT 2 cut(s) 316, 487
HpyCH4IV ACGT 2 cut(s) 123, 1323
HpyCH4V TGCA 5 cut(s) 550, 742, 914, 980, 1227
HpyF10VI GCNNNNNNNGC 3 cut(s) 432, 793, 973
HpyF3I CTNAG 5 cut(s) 15, 371, 522, 696, 953
HpySE526I ACGT 2 cut(s) 123, 1323
Hsp92I GRCGYC 2 cut(s) 123, 300
HspAI GCGC 3 cut(s) 159, 232, 390
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 3 cut(s) 606, 673, 839
LguI GCTCTTC 1 cut(s) 416
LmnI GCTCC 6 cut(s) 209, 573, 880, 924, 1194, 1285
LweI GCATC 5 cut(s) 751, 766, 783, 901, 939
MaeI CTAG 2 cut(s) 812, 1265
MaeII ACGT 2 cut(s) 123, 1323
MaeIII GTNAC 2 cut(s) 183, 1210
MalI GATC 3 cut(s) 608, 675, 841
MbiI CCGCTC 1 cut(s) 204
MboI GATC 3 cut(s) 606, 673, 839
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 4 cut(s) 822, 844, 882, 1234
MlyI GAGTC 4 cut(s) 266, 272, 384, 1265
MmeI TCCRAC 3 cut(s) 171, 300, 1152
MroXI GAANNNNTTC 1 cut(s) 765
MseI TTAA 5 cut(s) 117, 753, 1023, 1065, 1149
MspA1I CMGCKG 1 cut(s) 1295
MspCI CTTAAG 2 cut(s) 752, 1064
MspI CCGG 3 cut(s) 652, 758, 1243
Mva1269I GAATGC 1 cut(s) 1229
MvnI CGCG 3 cut(s) 161, 232, 392
MwoI GCNNNNNNNGC 3 cut(s) 432, 793, 973
NdeII GATC 3 cut(s) 606, 673, 839
NlaIV GGNNCC 8 cut(s) 133, 223, 749, 859, 876, 1086, 1111, 1329
NmuCI GTSAC 1 cut(s) 1210
NspI RCATGY 2 cut(s) 791, 980
PaeI GCATGC 1 cut(s) 980
PagI TCATGA 2 cut(s) 471, 618
PciSI GCTCTTC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 306
PctI GAATGC 1 cut(s) 1229
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 3 cut(s) 329, 367, 1005
PinAI ACCGGT 1 cut(s) 1242
PkrI GCNGC 3 cut(s) 158, 268, 966
PleI GAGTC 4 cut(s) 266, 272, 383, 1265
PpsI GAGTC 4 cut(s) 266, 272, 383, 1265
Ppu21I YACGTR 1 cut(s) 1324
PpuMI RGGWCCY 1 cut(s) 748
Psp5II RGGWCCY 1 cut(s) 748
PspN4I GGNNCC 8 cut(s) 133, 223, 749, 859, 876, 1086, 1111, 1329
PspPI GGNCC 5 cut(s) 131, 221, 350, 748, 1109
PspPPI RGGWCCY 1 cut(s) 748
RsaI GTAC 2 cut(s) 602, 650
RsaNI GTAC 2 cut(s) 601, 649
SapI GCTCTTC 1 cut(s) 416
SaqAI TTAA 5 cut(s) 117, 753, 1023, 1065, 1149
SatI GCNGC 3 cut(s) 157, 267, 965
Sau3AI GATC 3 cut(s) 606, 673, 839
Sau96I GGNCC 5 cut(s) 131, 221, 350, 748, 1109
SchI GAGTC 4 cut(s) 266, 272, 384, 1265
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 5 cut(s) 751, 766, 783, 901, 939
SinI GGWCC 1 cut(s) 748
SmlI CTYRAG 3 cut(s) 102, 752, 1064
SmoI CTYRAG 3 cut(s) 102, 752, 1064
SphI GCATGC 1 cut(s) 980
Sse9I AATT 4 cut(s) 822, 844, 882, 1234
SspMI CTAG 2 cut(s) 812, 1265
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 2 cut(s) 316, 487
TaiI ACGT 2 cut(s) 126, 1326
TaqI TCGA 3 cut(s) 111, 363, 842
TasI AATT 4 cut(s) 822, 844, 882, 1234
TatI WGTACW 1 cut(s) 600
TauI GCSGC 3 cut(s) 159, 269, 967
TfiI GAWTC 3 cut(s) 329, 367, 1005
Tru1I TTAA 5 cut(s) 117, 753, 1023, 1065, 1149
Tru9I TTAA 5 cut(s) 117, 753, 1023, 1065, 1149
TscAI CASTG 6 cut(s) 433, 673, 697, 1051, 1111, 1120
TseFI GTSAC 1 cut(s) 1210
Tsp45I GTSAC 1 cut(s) 1210
TspDTI ATGAA 8 cut(s) 159, 453, 479, 530, 607, 635, 843, 1335
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 6 cut(s) 433, 673, 697, 1051, 1111, 1120
Vha464I CTTAAG 2 cut(s) 752, 1064
VneI GTGCAC 1 cut(s) 548
VpaK11BI GGWCC 1 cut(s) 748
XapI RAATTY 2 cut(s) 844, 882
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 2 cut(s) 791, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmiI GTMKAC 1 cut(s) 934
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 2 cut(s) 812, 1265
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.