MD06G1183400.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007510
Physical Location & Seq
Reverse (-)
32178043 .. 32180916
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1183400.v1.1.491

Sequence Viewer

Length: 1410 bp
ATGGTGACGTCATCTCAGAAGAAAACGCTCCACTCCCTGCTGGCCGCGAAGCTCAGCAGCCTCACAATCCCGCCGTCCTCCGCCCCATCCTCCTCATCCCCACCTCAAGACTTCGACTTTAACGAAATCTTCGGCCCCACAGCCACCACCCCCAACGACTCATCAACCTCCGCCGCCGCCTCATTCCCGCCTCCGCCGCTAGTCATCCACAACCGCTCTCACTCCTTCGTGGGCCCCTCCCCGCGCTTCACTCCCACTCCCTACTCCCTTCCCTTCACCTCCGACTTCCTCGATTCCCAGAGCGATGACGCCGAAGCGGAAACCCCAGTCGGAACCGAGAATCGGAATGGTGGGGAATCAGAGAAGACCGAGCTCGGAGCTCGGAAGTTTGGGCCTGCGGATTTTGAGATGCTGAGAGTTGTGGGTCAGGGCGCGTTTGGGAAGGTCTTTCAAGTGAGAAAGAAGAGCGACGACGACAGTAACGGCGGCGGTGACGGCGGTGATGGGCTTTTTGCCATGAAGGTTATGAGGAAGGACACAATCATAAAGAAGAACCATGTTGATTACATGAAAGCTGAGAGAGATATTCTCACTAAGGTTGTGCACCCCTTCATTGTTCAGCTCCGCTACTCTTTCCAGACCAACTCTAAGCTTTATTTGATCATGGATTTTATAAACGGAGGGCATCTCTTCTTTCATCTATACCGGCAGGGAATCTTCAGCGAGGATCAGGCGAGATTTTATACTGCTGAGATAGTGTCTGCTGTTGCACATCTTCACAAGTGCGGGATTGTCCATCGGGACCTTAAACCTGAAAATGTTCTTGTGGATTCTGATGGGCATGTCATGCTAACTGATTTTGGGCTAGCAAAGGAAATTGACGAAGCCAGCAGATCGAATTCAATGTGTGGCACCACAGAGTACATGGCTCCAGAAATTTTGCTGTCTAAAGGCCACAACAAAGATGCGGATTGGTGGAGCATCGGTATCCTTTTGTATGAGATGCTAAAGGGGCAGCCACCATTTACACACGCCAACAGAAAGAAACTTCAGGAGAGAATCATCAAGGAGAAAGTTAAACTCCCGTCGTATCTGACCAGCGAAGCTCACTCTCTGCTGAAAGGATTGCTACAAAAGGATCCGTCACAGAGGTTAGGTAACGGGCCGAATGGAGGGGATAACGTCAAAAGCCATAAATGGTTTCGATCAATCAACTGGAAGAAGTTGGAGGCCAGAGAAGTGCTCCCAAAGTTCAAACCAGATGTGAGCGGAAAAGACTGCACAGCCAACTTTGACCGGTGCTGGACTACAATGCCTCTGGAAGACTCACCCGCTCCTACACCAACTGCGGGTGAGCATTTCCAGGGGTACACTTACGTGGCACCTAACCCGTGGCTTTCGTCGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

470

Amino Acids

52.0

Weight (kDa)

6.98

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 135 - 401 2.3e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 136 - 384 1.1e-38 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 216 - 300 1.3e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 244 - 384 1.1e-09 Kinase-like
Pkinase_fungal PF17667 253 - 324 3.9e-08 Fungal protein kinase
Pkinase_C PF00433 422 - 459 3.4e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 674
AatII GACGTC 1 cut(s) 11
AccB1I GGYRCC 2 cut(s) 911, 1381
AccBSI CCGCTC 3 cut(s) 216, 1269, 1334
AccII CGCG 3 cut(s) 47, 244, 434
AclWI GGATC 3 cut(s) 735, 1133, 1146
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 2 cut(s) 898, 936
AcuI CTGAAG 2 cut(s) 703, 1034
AcyI GRCGYC 2 cut(s) 8, 309
AfaI GTAC 2 cut(s) 923, 1370
AfiI CCNNNNNNNGG 3 cut(s) 342, 1172, 1349
AgeI ACCGGT 1 cut(s) 1296
AgsI TTSAA 3 cut(s) 452, 903, 1255
AjnI CCWGG 1 cut(s) 1362
AleI CACNNNNGTG 1 cut(s) 1376
AluBI AGCT 7 cut(s) 52, 373, 380, 575, 622, 652, 1106
AluI AGCT 7 cut(s) 52, 373, 380, 575, 622, 652, 1106
Alw21I GWGCWC 4 cut(s) 375, 382, 606, 1245
Alw44I GTGCAC 1 cut(s) 602
AlwI GGATC 3 cut(s) 735, 1133, 1146
AoxI GGCC 7 cut(s) 42, 133, 232, 392, 952, 1163, 1230
ApaI GGGCCC 1 cut(s) 236
ApaLI GTGCAC 1 cut(s) 602
ApeKI GCWGC 2 cut(s) 57, 1015
ApoI RAATTY 2 cut(s) 898, 936
AsiGI ACCGGT 1 cut(s) 1296
Asp700I GAANNNNTTC 1 cut(s) 819
AspLEI GCGC 2 cut(s) 246, 434
AspS9I GGNCC 6 cut(s) 134, 232, 233, 392, 802, 1163
AsuHPI GGTGA 6 cut(s) 16, 268, 503, 512, 1320, 1364
AsuNHI GCTAGC 1 cut(s) 865
AvaII GGWCC 1 cut(s) 802
BaeGI GKGCMC 2 cut(s) 236, 606
BamHI GGATCC 1 cut(s) 1138
BanI GGYRCC 2 cut(s) 911, 1381
BanII GRGCYC 3 cut(s) 236, 375, 382
BbsI GAAGAC 2 cut(s) 371, 1329
Bbv12I GWGCWC 4 cut(s) 375, 382, 606, 1245
BbvI GCAGC 2 cut(s) 69, 1027
BccI CCATC 4 cut(s) 94, 497, 804, 830
BceAI ACGGC 3 cut(s) 58, 499, 511
BciT130I CCWGG 1 cut(s) 1364
BciVI GTATCC 1 cut(s) 998
BclI TGATCA 1 cut(s) 660
BfaI CTAG 2 cut(s) 200, 866
BfuI GTATCC 1 cut(s) 998
BisI GCNGC 7 cut(s) 45, 58, 174, 177, 197, 487, 1016
BlpI GCTNAGC 1 cut(s) 53
BlsI GCNGC 7 cut(s) 46, 59, 175, 178, 198, 488, 1017
Bme1390I CCNGG 1 cut(s) 1364
Bme18I GGWCC 1 cut(s) 802
BmgT120I GGNCC 6 cut(s) 134, 232, 233, 392, 802, 1163
BmiI GGNNCC 9 cut(s) 136, 234, 235, 334, 803, 913, 930, 1140, 1383
BmrFI CCNGG 1 cut(s) 1364
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 5 cut(s) 399, 694, 955, 990, 993
BmtI GCTAGC 1 cut(s) 869
BmuI ACTGGG 1 cut(s) 320
BpiI GAAGAC 2 cut(s) 371, 1329
BplI GAGNNNNNCTC 6 cut(s) 573, 605, 672, 704, 1227, 1259
BpmI CTGGAG 1 cut(s) 915
Bpu1102I GCTNAGC 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 90
BsaAI YACGTR 1 cut(s) 1378
BsaHI GRCGYC 2 cut(s) 8, 309
BsaJI CCNNGG 2 cut(s) 1363, 1391
BsaWI WCCGGW 1 cut(s) 1296
BsaXI ACNNNNNCTCC 4 cut(s) 241, 271, 970, 1000
Bsc4I CCNNNNNNNGG 3 cut(s) 342, 1172, 1349
Bse118I RCCGGY 2 cut(s) 705, 1296
Bse1I ACTGG 2 cut(s) 326, 1220
BseBI CCWGG 1 cut(s) 1364
BseDI CCNNGG 2 cut(s) 1363, 1391
BseGI GGATG 3 cut(s) 86, 95, 204
BseLI CCNNNNNNNGG 3 cut(s) 342, 1172, 1349
BseMII CTCAG 5 cut(s) 29, 67, 404, 567, 741
BseNI ACTGG 2 cut(s) 326, 1220
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 2 cut(s) 236, 606
BseXI GCAGC 2 cut(s) 69, 1027
BsgI GTGCAG 1 cut(s) 1264
Bsh1236I CGCG 3 cut(s) 47, 244, 434
BshFI GGCC 7 cut(s) 44, 135, 234, 394, 954, 1165, 1232
BshNI GGYRCC 2 cut(s) 911, 1381
BshTI ACCGGT 1 cut(s) 1296
BsiHKAI GWGCWC 4 cut(s) 375, 382, 606, 1245
BsiSI CCGG 2 cut(s) 706, 1297
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 3 cut(s) 342, 1172, 1349
BsmFI GGGAC 1 cut(s) 815
BsnI GGCC 7 cut(s) 44, 135, 234, 394, 954, 1165, 1232
Bsp120I GGGCCC 1 cut(s) 232
Bsp1286I GDGCHC 5 cut(s) 236, 375, 382, 606, 1245
Bsp143I GATC 5 cut(s) 660, 727, 893, 1138, 1205
Bsp1720I GCTNAGC 1 cut(s) 53
BspANI GGCC 7 cut(s) 44, 135, 234, 394, 954, 1165, 1232
BspCNI CTCAG 5 cut(s) 28, 66, 405, 568, 742
BspFNI CGCG 3 cut(s) 47, 244, 434
BspLI GGNNCC 9 cut(s) 136, 234, 235, 334, 803, 913, 930, 1140, 1383
BspOI GCTAGC 1 cut(s) 869
BspPI GGATC 3 cut(s) 735, 1133, 1146
BspQI GCTCTTC 1 cut(s) 458
BspT107I GGYRCC 2 cut(s) 911, 1381
BsrBI CCGCTC 3 cut(s) 216, 1269, 1334
BsrFI RCCGGY 2 cut(s) 705, 1296
BsrI ACTGG 2 cut(s) 326, 1220
BssAI RCCGGY 2 cut(s) 705, 1296
BssECI CCNNGG 2 cut(s) 1363, 1391
BssMI GATC 5 cut(s) 660, 727, 893, 1138, 1205
BssNI GRCGYC 2 cut(s) 8, 309
Bst2UI CCWGG 1 cut(s) 1364
Bst4CI ACNGT 1 cut(s) 479
Bst6I CTCTTC 2 cut(s) 458, 695
BstACI GRCGYC 2 cut(s) 8, 309
BstAPI GCANNNNNTGC 1 cut(s) 847
BstBAI YACGTR 1 cut(s) 1378
BstC8I GCNNGC 4 cut(s) 42, 396, 867, 889
BstDEI CTNAG 7 cut(s) 15, 53, 413, 576, 594, 648, 750
BstDSI CCRYGG 1 cut(s) 1391
BstF5I GGATG 3 cut(s) 86, 95, 204
BstFNI CGCG 3 cut(s) 47, 244, 434
BstHHI GCGC 2 cut(s) 246, 434
BstKTI GATC 5 cut(s) 663, 730, 896, 1141, 1208
BstMBI GATC 5 cut(s) 660, 727, 893, 1138, 1205
BstMWI GCNNNNNNNGC 4 cut(s) 196, 495, 847, 1012
BstNI CCWGG 1 cut(s) 1364
BstNSI RCATGY 1 cut(s) 845
BstSCI CCNGG 1 cut(s) 1362
BstSLI GKGCMC 2 cut(s) 236, 606
BstUI CGCG 3 cut(s) 47, 244, 434
BstV1I GCAGC 2 cut(s) 69, 1027
BstV2I GAAGAC 2 cut(s) 371, 1329
BstX2I RGATCY 1 cut(s) 1138
BstYI RGATCY 1 cut(s) 1138
BsuI GTATCC 1 cut(s) 998
BsuRI GGCC 7 cut(s) 44, 135, 234, 394, 954, 1165, 1232
BtgI CCRYGG 1 cut(s) 1391
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 3 cut(s) 86, 95, 204
Cac8I GCNNGC 4 cut(s) 42, 396, 867, 889
CfoI GCGC 2 cut(s) 246, 434
Cfr10I RCCGGY 2 cut(s) 705, 1296
Cfr13I GGNCC 6 cut(s) 134, 232, 233, 392, 802, 1163
CseI GACGC 1 cut(s) 317
Csp6I GTAC 2 cut(s) 922, 1369
CspAI ACCGGT 1 cut(s) 1296
CviAII CATG 7 cut(s) 517, 557, 568, 664, 842, 847, 925
CviQI GTAC 2 cut(s) 922, 1369
DdeI CTNAG 7 cut(s) 15, 53, 413, 576, 594, 648, 750
DpnI GATC 5 cut(s) 662, 729, 895, 1140, 1207
DpnII GATC 5 cut(s) 660, 727, 893, 1138, 1205
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 458, 695
EarI CTCTTC 2 cut(s) 458, 695
EciI GGCGGA 3 cut(s) 70, 160, 183
Ecl136II GAGCTC 2 cut(s) 373, 380
Eco24I GRGCYC 3 cut(s) 236, 375, 382
Eco47I GGWCC 1 cut(s) 802
Eco53kI GAGCTC 2 cut(s) 373, 380
Eco57I CTGAAG 2 cut(s) 703, 1034
EcoICRI GAGCTC 2 cut(s) 373, 380
EcoO109I RGGNCCY 2 cut(s) 233, 802
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 1 cut(s) 1362
EcoT38I GRGCYC 3 cut(s) 236, 375, 382
FaeI CATG 7 cut(s) 520, 560, 571, 667, 845, 850, 928
FaqI GGGAC 1 cut(s) 815
FatI CATG 7 cut(s) 516, 556, 567, 663, 841, 846, 924
FauI CCCGC 6 cut(s) 78, 195, 249, 779, 1339, 1342
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 7 cut(s) 45, 58, 174, 177, 197, 487, 1016
FokI GGATG 3 cut(s) 73, 82, 191
FriOI GRGCYC 3 cut(s) 236, 375, 382
Fsp4HI GCNGC 7 cut(s) 45, 58, 174, 177, 197, 487, 1016
FspBI CTAG 2 cut(s) 200, 866
GlaI GCGC 2 cut(s) 245, 433
GluI GCNGC 7 cut(s) 45, 58, 174, 177, 197, 487, 1016
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 7 cut(s) 44, 135, 234, 394, 954, 1165, 1232
HapII CCGG 2 cut(s) 706, 1297
HgaI GACGC 1 cut(s) 317
HhaI GCGC 2 cut(s) 246, 434
Hin1I GRCGYC 2 cut(s) 8, 309
Hin1II CATG 7 cut(s) 520, 560, 571, 667, 845, 850, 928
Hin6I GCGC 2 cut(s) 244, 432
HinP1I GCGC 2 cut(s) 244, 432
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 8 cut(s) 158, 293, 340, 356, 714, 830, 1059, 1325
HpaII CCGG 2 cut(s) 706, 1297
HphI GGTGA 6 cut(s) 16, 268, 503, 512, 1320, 1364
Hpy166II GTNNAC 2 cut(s) 604, 1371
Hpy188I TCNGA 9 cut(s) 18, 283, 332, 345, 361, 377, 384, 835, 1095
Hpy188III TCNNGA 6 cut(s) 107, 637, 800, 932, 1052, 1319
Hpy8I GTNNAC 2 cut(s) 604, 1371
Hpy99I CGWCG 4 cut(s) 473, 476, 1090, 1405
HpyAV CCTTC 7 cut(s) 235, 278, 283, 436, 514, 526, 619
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4IV ACGT 3 cut(s) 8, 1182, 1377
HpyCH4V TGCA 3 cut(s) 604, 770, 1281
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 495, 847, 1012
HpyF3I CTNAG 7 cut(s) 15, 53, 413, 576, 594, 648, 750
HpySE526I ACGT 3 cut(s) 8, 1182, 1377
Hsp92I GRCGYC 2 cut(s) 8, 309
Hsp92II CATG 7 cut(s) 520, 560, 571, 667, 845, 850, 928
HspAI GCGC 2 cut(s) 244, 432
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 5 cut(s) 660, 727, 893, 1138, 1205
LguI GCTCTTC 1 cut(s) 458
LmnI GCTCC 7 cut(s) 33, 377, 627, 934, 978, 1248, 1339
Lsp1109I GCAGC 2 cut(s) 69, 1027
LweI GCATC 5 cut(s) 399, 694, 955, 990, 993
MaeI CTAG 2 cut(s) 200, 866
MaeII ACGT 3 cut(s) 8, 1182, 1377
MaeIII GTNAC 5 cut(s) 4, 479, 491, 1143, 1157
MalI GATC 5 cut(s) 662, 729, 895, 1140, 1207
MbiI CCGCTC 3 cut(s) 216, 1269, 1334
MboI GATC 5 cut(s) 660, 727, 893, 1138, 1205
MflI RGATCY 1 cut(s) 1138
MhlI GDGCHC 5 cut(s) 236, 375, 382, 606, 1245
MluCI AATT 3 cut(s) 876, 898, 936
MlyI GAGTC 2 cut(s) 152, 1319
MmeI TCCRAC 3 cut(s) 306, 310, 1206
MroXI GAANNNNTTC 1 cut(s) 819
MseI TTAA 3 cut(s) 120, 807, 1077
MslI CAYNNNNRTG 1 cut(s) 1376
MspI CCGG 2 cut(s) 706, 1297
MspR9I CCNGG 1 cut(s) 1364
MvaI CCWGG 1 cut(s) 1364
MvnI CGCG 3 cut(s) 47, 244, 434
MwoI GCNNNNNNNGC 4 cut(s) 196, 495, 847, 1012
NdeII GATC 5 cut(s) 660, 727, 893, 1138, 1205
NheI GCTAGC 1 cut(s) 865
NlaIII CATG 7 cut(s) 520, 560, 571, 667, 845, 850, 928
NlaIV GGNNCC 9 cut(s) 136, 234, 235, 334, 803, 913, 930, 1140, 1383
NmuCI GTSAC 3 cut(s) 4, 491, 1143
NspI RCATGY 1 cut(s) 845
OliI CACNNNNGTG 1 cut(s) 1376
PciSI GCTCTTC 1 cut(s) 458
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 819
PfeI GAWTC 6 cut(s) 293, 340, 356, 714, 830, 1059
PinAI ACCGGT 1 cut(s) 1296
PkrI GCNGC 7 cut(s) 46, 59, 175, 178, 198, 488, 1017
PleI GAGTC 2 cut(s) 152, 1319
PpsI GAGTC 2 cut(s) 152, 1319
Ppu21I YACGTR 1 cut(s) 1378
PpuMI RGGWCCY 1 cut(s) 802
PsiI TTATAA 1 cut(s) 674
Psp124BI GAGCTC 2 cut(s) 375, 382
Psp5II RGGWCCY 1 cut(s) 802
Psp6I CCWGG 1 cut(s) 1362
PspGI CCWGG 1 cut(s) 1362
PspN4I GGNNCC 9 cut(s) 136, 234, 235, 334, 803, 913, 930, 1140, 1383
PspOMI GGGCCC 1 cut(s) 232
PspPI GGNCC 6 cut(s) 134, 232, 233, 392, 802, 1163
PspPPI RGGWCCY 1 cut(s) 802
PsuI RGATCY 1 cut(s) 1138
RsaI GTAC 2 cut(s) 923, 1370
RsaNI GTAC 2 cut(s) 922, 1369
RseI CAYNNNNRTG 1 cut(s) 1376
SacI GAGCTC 2 cut(s) 375, 382
SapI GCTCTTC 1 cut(s) 458
SaqAI TTAA 3 cut(s) 120, 807, 1077
SatI GCNGC 7 cut(s) 45, 58, 174, 177, 197, 487, 1016
Sau3AI GATC 5 cut(s) 660, 727, 893, 1138, 1205
Sau96I GGNCC 6 cut(s) 134, 232, 233, 392, 802, 1163
SchI GAGTC 2 cut(s) 152, 1319
ScrFI CCNGG 1 cut(s) 1364
SduI GDGCHC 5 cut(s) 236, 375, 382, 606, 1245
SfaNI GCATC 5 cut(s) 399, 694, 955, 990, 993
SinI GGWCC 1 cut(s) 802
SmiMI CAYNNNNRTG 1 cut(s) 1376
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
Sse9I AATT 3 cut(s) 876, 898, 936
SspMI CTAG 2 cut(s) 200, 866
SstI GAGCTC 2 cut(s) 375, 382
StyD4I CCNGG 1 cut(s) 1362
TaaI ACNGT 1 cut(s) 479
TaiI ACGT 3 cut(s) 11, 1185, 1380
TaqI TCGA 5 cut(s) 114, 291, 896, 1204, 1403
TaqII GACCGA 1 cut(s) 383
TasI AATT 3 cut(s) 876, 898, 936
TatI WGTACW 1 cut(s) 921
TauI GCSGC 5 cut(s) 47, 176, 179, 199, 489
TfiI GAWTC 6 cut(s) 293, 340, 356, 714, 830, 1059
Tru1I TTAA 3 cut(s) 120, 807, 1077
Tru9I TTAA 3 cut(s) 120, 807, 1077
TseFI GTSAC 3 cut(s) 4, 491, 1143
TseI GCWGC 2 cut(s) 57, 1015
Tsp45I GTSAC 3 cut(s) 4, 491, 1143
TspDTI ATGAA 4 cut(s) 533, 584, 601, 686
TspGWI ACGGA 2 cut(s) 693, 1131
VneI GTGCAC 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 2 cut(s) 898, 936
XceI RCATGY 1 cut(s) 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 922
XmnI GAANNNNTTC 1 cut(s) 819
XspI CTAG 2 cut(s) 200, 866
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.