Rroxscaffold_3G00273230

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007510
Physical Location & Seq
Forward (+)
65086070 .. 65088780
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00273230.1

Sequence Viewer

Length: 1278 bp
ATGGTGTCCTCCTCTCAGAACAATAACAAAACCCTCCATTCCCTCCTTGCCTCAAACCTCACCAAGCTCACAATCCCACCTTCCTCCTCTTCTCCGCCTCAAGACTTCGACTTTAACGACGTCTTCGGCCCCCTACCGCCTCCAACGACGGCGGCGCGTCCACGTCATACTCGCCGTCGTTACTCGTCATCCACAACCGCTCCCACTCCTTCGTCGGCCCCTCCCCGCGCTTCACTCCCTCCTCCTCCTCCTCCCTCCTCCCCTTCACCGCCGCCGAATCCGACTCCGAGGACGACGAAAACGACGCCAGTCGTCGGCAAGGGCGCGTTCGGGAAGGTGTTTCAGGTGAGGAAGAAGAGCAGTGATGGCGATGAAGATGGTGATGGGCTTTATGCCATGAAGGTTATGAGGAAGGAGACTGTTATCAAGAAGAACCATGTGGATTACATGAAGGCTGAGAGGGATATTCTCACAAAAGTTGTGCACCCTTTTATTGTTCAGCTCCGCTACTCTTTTCAGACCAGGTGTAAGCTGTACTTGATCATGGATTTCATGAATGGAGGGCATTTGTTCTATCATTTGTACCGCCAGGGGATATTCAGTGAGGATCAGGCGAGGTTTTATACTGCTGAGATAGTGTCTGCTGTTTCACATCTTCACAAGTGCGGGATTGTGCATCGGGACCTTAAGCCGGAAAATGTTCTCATGGATGCGGATGGGCATGTGATGCTGACGGATTTTGGACTAGCAAAGGAAATTGATGAAGATAGCAGATCGAATTCAATGTGTGGAACCACCGAATACATGGCTCCTGAAATTCTGCTGTCTAAAGGCCACAACAAAGATGCAGATTGGTGGAGCATTGGTATACTTTTGTATGAGATGCTAAGTGGTCAGCCACCATTTACGCATGCAAATAGAAAGAAACTTCAGGAGAGAATCATCAAGGAGAAAGTCAAACTTCCACCTTACCTTACCACTGAAGCTCACTCTTTGCTCAAAGGATTGCTACAGAAGGAACCATCCCAAAGGTTAGGTAGTGGCCCCAGTGGAGGTGATAATATCAAAAGTCATAAATGGTTTAAAACAATCAACTGGAAGAAGTTGGAGGCCAGAGAACTGCTCCCAAAGTTCAAGCCAGATGTGACTGGAAAAGAATGCACGGCCAATTTTGACCGGTGCTGGACAACAATGCCTCTAGATGACTCACCAGCTCCTACTCCAACAGCGGGTTTTAAGCTACATTCTTATTTCATTTCTGGTTCTGCTTTGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

425

Amino Acids

47.45

Weight (kDa)

9.4

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 103 - 361 1.2e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 103 - 344 5.1e-39 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 179 - 260 2.5e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 203 - 343 8.7e-10 Kinase-like
Pkinase_fungal PF17667 212 - 284 1.9e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
AccBSI CCGCTC 1 cut(s) 200
AccI GTMKAC 1 cut(s) 868
AccII CGCG 3 cut(s) 157, 228, 326
AclWI GGATC 1 cut(s) 615
AcoI YGGCCR 1 cut(s) 1164
AcsI RAATTY 2 cut(s) 778, 816
AcuI CTGAAG 2 cut(s) 914, 1002
AcyI GRCGYC 2 cut(s) 120, 305
AfaI GTAC 2 cut(s) 536, 584
AfiI CCNNNNNNNGG 4 cut(s) 314, 691, 1052, 1229
AflII CTTAAG 1 cut(s) 686
AgeI ACCGGT 1 cut(s) 1176
AgsI TTSAA 2 cut(s) 783, 1135
AjiI CACGTC 1 cut(s) 164
AjnI CCWGG 2 cut(s) 521, 588
AluBI AGCT 6 cut(s) 67, 502, 532, 986, 1214, 1240
AluI AGCT 6 cut(s) 67, 502, 532, 986, 1214, 1240
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 1 cut(s) 410
Alw44I GTGCAC 1 cut(s) 482
AlwI GGATC 1 cut(s) 615
AoxI GGCC 6 cut(s) 127, 216, 832, 1042, 1110, 1164
ApaLI GTGCAC 1 cut(s) 482
ApoI RAATTY 2 cut(s) 778, 816
AsiGI ACCGGT 1 cut(s) 1176
Asp700I GAANNNNTTC 1 cut(s) 699
AspLEI GCGC 3 cut(s) 157, 230, 326
AspS9I GGNCC 4 cut(s) 128, 217, 682, 1043
AsuHPI GGTGA 6 cut(s) 52, 258, 358, 392, 1067, 1200
AvaII GGWCC 1 cut(s) 682
BaeGI GKGCMC 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 486
BccI CCATC 5 cut(s) 359, 371, 377, 710, 1030
BceAI ACGGC 3 cut(s) 159, 165, 1179
BciT130I CCWGG 2 cut(s) 523, 590
BclI TGATCA 1 cut(s) 540
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 2 cut(s) 746, 1199
BfmI CTRYAG 1 cut(s) 1010
BfrI CTTAAG 1 cut(s) 686
BisI GCNGC 2 cut(s) 153, 272
BlsI GCNGC 2 cut(s) 154, 273
Bme1390I CCNGG 2 cut(s) 523, 590
Bme18I GGWCC 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 164
BmgT120I GGNCC 4 cut(s) 128, 217, 682, 1043
BmiI GGNNCC 7 cut(s) 130, 219, 683, 793, 810, 1020, 1045
BmrFI CCNGG 2 cut(s) 523, 590
BmrI ACTGGG 1 cut(s) 1041
BmsI GCATC 5 cut(s) 685, 700, 717, 835, 873
BmuI ACTGGG 1 cut(s) 1041
BoxI GACNNNNGTC 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 115
BplI GAGNNNNNCTC 2 cut(s) 1107, 1139
BpuEI CTTGAG 1 cut(s) 84
BsaHI GRCGYC 2 cut(s) 120, 305
BsaJI CCNNGG 2 cut(s) 287, 589
BsaWI WCCGGW 1 cut(s) 1176
BsaXI ACNNNNNCTCC 4 cut(s) 184, 214, 850, 880
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 691, 1052, 1229
Bse118I RCCGGY 1 cut(s) 1176
Bse1I ACTGG 4 cut(s) 308, 1047, 1100, 1153
BseBI CCWGG 2 cut(s) 523, 590
BseDI CCNNGG 2 cut(s) 287, 589
BseGI GGATG 4 cut(s) 188, 715, 721, 1022
BseLI CCNNNNNNNGG 4 cut(s) 314, 691, 1052, 1229
BseMII CTCAG 3 cut(s) 29, 447, 621
BseNI ACTGG 4 cut(s) 308, 1047, 1100, 1153
BseRI GAGGAG 6 cut(s) 76, 231, 234, 237, 240, 247
BseSI GKGCMC 1 cut(s) 486
Bsh1236I CGCG 3 cut(s) 157, 228, 326
BshFI GGCC 6 cut(s) 129, 218, 834, 1044, 1112, 1166
BshTI ACCGGT 1 cut(s) 1176
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 2 cut(s) 692, 1177
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 4 cut(s) 314, 691, 1052, 1229
BsmAI GTCTC 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 695
BsmI GAATGC 1 cut(s) 1163
BsnI GGCC 6 cut(s) 129, 218, 834, 1044, 1112, 1166
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 3 cut(s) 540, 607, 773
BspANI GGCC 6 cut(s) 129, 218, 834, 1044, 1112, 1166
BspCNI CTCAG 3 cut(s) 28, 448, 622
BspFNI CGCG 3 cut(s) 157, 228, 326
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 7 cut(s) 130, 219, 683, 793, 810, 1020, 1045
BspPI GGATC 1 cut(s) 615
BspQI GCTCTTC 1 cut(s) 350
BspTI CTTAAG 1 cut(s) 686
BsrBI CCGCTC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 1176
BsrI ACTGG 4 cut(s) 308, 1047, 1100, 1153
BssAI RCCGGY 1 cut(s) 1176
BssECI CCNNGG 2 cut(s) 287, 589
BssMI GATC 3 cut(s) 540, 607, 773
BssNAI GTATAC 1 cut(s) 869
BssNI GRCGYC 2 cut(s) 120, 305
Bst1107I GTATAC 1 cut(s) 869
Bst2UI CCWGG 2 cut(s) 523, 590
Bst4CI ACNGT 1 cut(s) 421
Bst6I CTCTTC 2 cut(s) 94, 350
BstACI GRCGYC 2 cut(s) 120, 305
BstAFI CTTAAG 1 cut(s) 686
BstAPI GCANNNNNTGC 1 cut(s) 727
BstC8I GCNNGC 1 cut(s) 912
BstDEI CTNAG 4 cut(s) 15, 456, 630, 887
BstF5I GGATG 4 cut(s) 188, 715, 721, 1022
BstFNI CGCG 3 cut(s) 157, 228, 326
BstHHI GCGC 3 cut(s) 157, 230, 326
BstKTI GATC 3 cut(s) 543, 610, 776
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 3 cut(s) 540, 607, 773
BstMWI GCNNNNNNNGC 2 cut(s) 366, 727
BstNI CCWGG 2 cut(s) 523, 590
BstNSI RCATGY 2 cut(s) 725, 914
BstPAI GACNNNNGTC 1 cut(s) 308
BstSCI CCNGG 2 cut(s) 521, 588
BstSFI CTRYAG 1 cut(s) 1010
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 3 cut(s) 157, 228, 326
BstV2I GAAGAC 1 cut(s) 115
BstZ17I GTATAC 1 cut(s) 869
BsuRI GGCC 6 cut(s) 129, 218, 834, 1044, 1112, 1166
BtgZI GCGATG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 164
BtsCI GGATG 4 cut(s) 188, 715, 721, 1022
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 4 cut(s) 367, 607, 978, 1054
Cac8I GCNNGC 1 cut(s) 912
CciI TCATGA 1 cut(s) 552
CfoI GCGC 3 cut(s) 157, 230, 326
Cfr10I RCCGGY 1 cut(s) 1176
Cfr13I GGNCC 4 cut(s) 128, 217, 682, 1043
CseI GACGC 2 cut(s) 146, 313
CsiI ACCWGGT 1 cut(s) 521
Csp6I GTAC 2 cut(s) 535, 583
CspAI ACCGGT 1 cut(s) 1176
CviAII CATG 9 cut(s) 397, 437, 448, 544, 553, 706, 722, 805, 911
CviQI GTAC 2 cut(s) 535, 583
DdeI CTNAG 4 cut(s) 15, 456, 630, 887
DpnI GATC 3 cut(s) 542, 609, 775
DpnII GATC 3 cut(s) 540, 607, 773
DraI TTTAAA 1 cut(s) 1084
EaeI YGGCCR 1 cut(s) 1164
Eam1104I CTCTTC 2 cut(s) 94, 350
EarI CTCTTC 2 cut(s) 94, 350
EciI GGCGGA 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 682
Eco57I CTGAAG 2 cut(s) 914, 1002
EcoO109I RGGNCCY 1 cut(s) 682
EcoRI GAATTC 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 521, 588
FaeI CATG 9 cut(s) 400, 440, 451, 547, 556, 709, 725, 808, 914
FalI AAGNNNNNCTT 4 cut(s) 521, 553, 945, 977
FaqI GGGAC 1 cut(s) 695
FatI CATG 9 cut(s) 396, 436, 447, 543, 552, 705, 721, 804, 910
FauI CCCGC 3 cut(s) 233, 659, 1222
FbaI TGATCA 1 cut(s) 540
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 2 cut(s) 153, 272
FokI GGATG 4 cut(s) 175, 722, 728, 1009
Fsp4HI GCNGC 2 cut(s) 153, 272
FspBI CTAG 2 cut(s) 746, 1199
GlaI GCGC 3 cut(s) 156, 229, 325
GluI GCNGC 2 cut(s) 153, 272
HaeIII GGCC 6 cut(s) 129, 218, 834, 1044, 1112, 1166
HapII CCGG 2 cut(s) 692, 1177
HgaI GACGC 2 cut(s) 146, 313
HhaI GCGC 3 cut(s) 157, 230, 326
Hin1I GRCGYC 2 cut(s) 120, 305
Hin1II CATG 9 cut(s) 400, 440, 451, 547, 556, 709, 725, 808, 914
Hin6I GCGC 3 cut(s) 155, 228, 324
HinP1I GCGC 3 cut(s) 155, 228, 324
HinfI GANTC 4 cut(s) 277, 283, 939, 1205
HpaII CCGG 2 cut(s) 692, 1177
HphI GGTGA 6 cut(s) 52, 258, 358, 392, 1067, 1200
Hpy166II GTNNAC 3 cut(s) 161, 484, 869
Hpy188I TCNGA 4 cut(s) 18, 282, 288, 519
Hpy188III TCNNGA 8 cut(s) 101, 331, 427, 553, 680, 812, 932, 1199
Hpy8I GTNNAC 3 cut(s) 161, 484, 869
Hpy99I CGWCG 7 cut(s) 122, 151, 180, 217, 298, 307, 317
HpyAV CCTTC 8 cut(s) 90, 219, 273, 328, 394, 406, 445, 1009
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 2 cut(s) 120, 163
HpyCH4V TGCA 5 cut(s) 484, 676, 848, 914, 1161
HpyF10VI GCNNNNNNNGC 2 cut(s) 366, 727
HpyF3I CTNAG 4 cut(s) 15, 456, 630, 887
HpySE526I ACGT 2 cut(s) 120, 163
Hsp92I GRCGYC 2 cut(s) 120, 305
Hsp92II CATG 9 cut(s) 400, 440, 451, 547, 556, 709, 725, 808, 914
HspAI GCGC 3 cut(s) 155, 228, 324
Ksp22I TGATCA 1 cut(s) 540
Kzo9I GATC 3 cut(s) 540, 607, 773
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 6 cut(s) 205, 507, 814, 858, 1128, 1219
LweI GCATC 5 cut(s) 685, 700, 717, 835, 873
MabI ACCWGGT 1 cut(s) 521
MaeI CTAG 2 cut(s) 746, 1199
MaeII ACGT 2 cut(s) 120, 163
MaeIII GTNAC 2 cut(s) 179, 1144
MalI GATC 3 cut(s) 542, 609, 775
MbiI CCGCTC 1 cut(s) 200
MboI GATC 3 cut(s) 540, 607, 773
MboII GAAGA 9 cut(s) 81, 115, 364, 367, 386, 442, 647, 776, 1111
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 4 cut(s) 756, 778, 816, 1168
MlyI GAGTC 2 cut(s) 277, 1199
MmeI TCCRAC 4 cut(s) 167, 305, 1086, 1247
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 4 cut(s) 114, 687, 1083, 1236
MspA1I CMGCKG 1 cut(s) 1229
MspCI CTTAAG 1 cut(s) 686
MspI CCGG 2 cut(s) 692, 1177
MspR9I CCNGG 2 cut(s) 523, 590
Mva1269I GAATGC 1 cut(s) 1163
MvaI CCWGG 2 cut(s) 523, 590
MvnI CGCG 3 cut(s) 157, 228, 326
MwoI GCNNNNNNNGC 2 cut(s) 366, 727
NdeII GATC 3 cut(s) 540, 607, 773
NlaIII CATG 9 cut(s) 400, 440, 451, 547, 556, 709, 725, 808, 914
NlaIV GGNNCC 7 cut(s) 130, 219, 683, 793, 810, 1020, 1045
NmuCI GTSAC 1 cut(s) 1144
NspI RCATGY 2 cut(s) 725, 914
PaeI GCATGC 1 cut(s) 914
PagI TCATGA 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 350
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 299
PctI GAATGC 1 cut(s) 1163
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 2 cut(s) 277, 939
PinAI ACCGGT 1 cut(s) 1176
PkrI GCNGC 2 cut(s) 154, 273
PleI GAGTC 2 cut(s) 277, 1199
PpsI GAGTC 2 cut(s) 277, 1199
PpuMI RGGWCCY 1 cut(s) 682
PshAI GACNNNNGTC 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 682
Psp6I CCWGG 2 cut(s) 521, 588
PspGI CCWGG 2 cut(s) 521, 588
PspN4I GGNNCC 7 cut(s) 130, 219, 683, 793, 810, 1020, 1045
PspPI GGNCC 4 cut(s) 128, 217, 682, 1043
PspPPI RGGWCCY 1 cut(s) 682
RsaI GTAC 2 cut(s) 536, 584
RsaNI GTAC 2 cut(s) 535, 583
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 4 cut(s) 114, 687, 1083, 1236
SatI GCNGC 2 cut(s) 153, 272
Sau3AI GATC 3 cut(s) 540, 607, 773
Sau96I GGNCC 4 cut(s) 128, 217, 682, 1043
SchI GAGTC 2 cut(s) 277, 1199
ScrFI CCNGG 2 cut(s) 523, 590
SduI GDGCHC 1 cut(s) 486
SexAI ACCWGGT 1 cut(s) 521
SfaNI GCATC 5 cut(s) 685, 700, 717, 835, 873
SfcI CTRYAG 1 cut(s) 1010
SinI GGWCC 1 cut(s) 682
SmlI CTYRAG 2 cut(s) 99, 686
SmoI CTYRAG 2 cut(s) 99, 686
SphI GCATGC 1 cut(s) 914
Sse9I AATT 4 cut(s) 756, 778, 816, 1168
SspMI CTAG 2 cut(s) 746, 1199
StyD4I CCNGG 2 cut(s) 521, 588
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 2 cut(s) 123, 166
TaqI TCGA 2 cut(s) 108, 776
TasI AATT 4 cut(s) 756, 778, 816, 1168
TatI WGTACW 1 cut(s) 534
TauI GCSGC 2 cut(s) 155, 274
TfiI GAWTC 2 cut(s) 277, 939
Tru1I TTAA 4 cut(s) 114, 687, 1083, 1236
Tru9I TTAA 4 cut(s) 114, 687, 1083, 1236
TscAI CASTG 4 cut(s) 367, 607, 985, 1054
TseFI GTSAC 1 cut(s) 1144
Tsp45I GTSAC 1 cut(s) 1144
TspDTI ATGAA 7 cut(s) 387, 413, 464, 541, 569, 777, 1243
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 4 cut(s) 367, 607, 985, 1054
Vha464I CTTAAG 1 cut(s) 686
VneI GTGCAC 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 2 cut(s) 778, 816
XbaI TCTAGA 1 cut(s) 1198
XceI RCATGY 2 cut(s) 725, 914
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XmiI GTMKAC 1 cut(s) 868
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 2 cut(s) 746, 1199
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.