FvH4_5g18230

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014049
Physical Location & Seq
Forward (+)
10480778 .. 10482348
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18230.t1

Sequence Viewer

Length: 1215 bp
ATGAGTAGTGTGTGCGCCGAGCAGCACAAGTTTCACACCTCCCACCACCTCCTTTCATCCAAGAAATCCCACCACCTCCTCAGGGAAGTTGACATTCCACCGAGAAAGCTCCTCACCCGCCGCTCTGCAGCTCACCAAGACATATCGTCCTCCCCCGACATGTACGACTCTCCACGCTTCTCTGCTTCCGACGAGGCCGTCCTCCGCAAGTTCCTCCCCTACAACTCTGACGACGATGACGACGACCCCTACTCTTCCGACCACTTCCGCATGTACGAGTTCAAGGTCCGCCGCTGCACCCGCAGCCGCAGCCACGACTGGACCGACTGCCCTTTCGCCCATCCCGGCGAAAAGGCCAGGCGCCGTGATCCCCGCTGCTATCACTACTCCGGCACAGTATGCGCCGACTACCGCCGCGGCGGGTGTAGCCGCGGCGACAGCTGTGAGTTTGCGCACGGTGTTTTCGAGTGTTGGCTGCATCCGGCGAGGTACAGAACCGAGGCATGCAAAGACGGCAAGCACTGTAAAAGGAAGGTTTGTTTCTTTGCTCACACCCCACATCAGCTCCGCATCTTGCCTGTGAATCACTCACGGAGCTCTTCACCTGATGAAGTAGTCATCTCTCCCGGCTCCGGTTGTTGCAAGAAGAGTAGCAAGTACCTGAATAACTCGGCCAACTCGCCGAGTTCGACTCACTGCTGTTCGTTCTGCCGTTCTGTGACTTGTTCCCCGACTTCCACTCTGCTCGGTATGTCGCCGATGTCTCCATACGGTGATGAATCCAAGTGTCACTTCTCTAGCGGGTTCAATCAGATGCATGCTGGTTCAGCTGGGATGCCCAGCTACAAAGATGTTCTTTCAGAGCTGATGACCTCTTTGGAGAAAATGAAAGTCAACAACATGGAAGCAGCTACTACACCAGTTTCATCACCAATCAGTTCTGTTGCATCAAGTTACCGGAATACGCCCTGGGTTGACGTCTCTTCATACAATGCTTTTGCTGAAGATCAGCAGCAACAGTTCATTCTTTCACCTAATTCTATTCCAAGCCCTTTATCAGCTGGAACTAATTTTTTCAGTCACCGTTGTAGTTCTGGAAATGGCTCAAGGAAAAGCTTGGTCTTCGATGAGCAGAACAAGGCCACTGACATTTATGGAGGAGGTTGTAATAGCCCCGACCCTGATCTTGGATGGGTCAACGATCTGTTGATGTAA

Protein Analysis

405

Amino Acids

45.07

Weight (kDa)

8.19

Isoelectric Point (pI)

64.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_AtC3H23 PF25512 88 - 121 2.3e-20 AtC3H23-like, CCCH zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014049)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03790 AT5G44260
fragaria_vesca FvH4_5g18230
malus_domestica MD09G1243500.v1.1 MD17G1235500.v1.1
prunus_persica Prupe.3G133500_v2.0.a1
pyrus_communis pycom09g16210
rosa_chinensis RchiOBHm_Chr2g0127531
rosa_laevigata RLG00000018959
rosa_multiflora Rmu_sc0004744.1_g000012
rosa_roxburghii Rroxscaffold_2G00116460
rosa_rugosa Rorug02G0273700
rosa_samantha Rh2AG325500 Rh2BG335000 Rh2CG312900 Rh2DG352600
rosa_wichuraiana Rw2G026400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AatII GACGTC 1 cut(s) 981
Acc16I TGCGCA 1 cut(s) 453
AccB1I GGYRCC 1 cut(s) 360
AccBSI CCGCTC 1 cut(s) 123
AccII CGCG 2 cut(s) 417, 432
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 672
AcuI CTGAAG 1 cut(s) 1023
AcyI GRCGYC 2 cut(s) 361, 978
AfaI GTAC 4 cut(s) 164, 275, 491, 659
AfiI CCNNNNNNNGG 3 cut(s) 82, 632, 1187
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 2 cut(s) 283, 808
AhdI GACNNNNNGTC 1 cut(s) 145
AjnI CCWGG 2 cut(s) 356, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 776, 808
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 2 cut(s) 768, 985
AlwI GGATC 1 cut(s) 362
AoxI GGCC 4 cut(s) 195, 354, 672, 1140
ApeKI GCWGC 9 cut(s) 22, 128, 294, 303, 309, 375, 475, 908, 1012
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AspLEI GCGC 4 cut(s) 17, 363, 404, 454
AspS9I GGNCC 2 cut(s) 286, 321
AsuC2I CCSGG 2 cut(s) 345, 627
AsuHPI GGTGA 7 cut(s) 106, 125, 594, 785, 921, 1023, 1073
AvaII GGWCC 2 cut(s) 286, 321
AxyI CCTNAGG 1 cut(s) 80
BanI GGYRCC 1 cut(s) 360
BanII GRGCYC 1 cut(s) 599
BbsI GAAGAC 1 cut(s) 1114
Bbv12I GWGCWC 1 cut(s) 599
BbvI GCAGC 9 cut(s) 34, 140, 281, 315, 321, 362, 462, 920, 1024
BccI CCATC 2 cut(s) 348, 1185
BceAI ACGGC 4 cut(s) 182, 348, 529, 696
BciT130I CCWGG 2 cut(s) 358, 970
BcnI CCSGG 2 cut(s) 345, 627
BcoDI GTCTC 2 cut(s) 768, 985
BfaI CTAG 1 cut(s) 798
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 364
Bme1390I CCNGG 4 cut(s) 345, 358, 627, 970
Bme18I GGWCC 2 cut(s) 286, 321
BmeRI GACNNNNNGTC 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 286, 321
BmiI GGNNCC 2 cut(s) 362, 631
BmrFI CCNGG 4 cut(s) 345, 358, 627, 970
BmsI GCATC 5 cut(s) 487, 579, 804, 825, 956
BpiI GAAGAC 1 cut(s) 1114
BplI GAGNNNNNCTC 2 cut(s) 676, 708
BpuEI CTTGAG 1 cut(s) 1090
BpuMI CCSGG 2 cut(s) 345, 627
BsaHI GRCGYC 2 cut(s) 361, 978
BsaJI CCNNGG 5 cut(s) 415, 430, 498, 968, 969
BsaWI WCCGGW 2 cut(s) 632, 957
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 632, 1187
Bse1I ACTGG 2 cut(s) 323, 920
Bse21I CCTNAGG 1 cut(s) 80
BseBI CCWGG 2 cut(s) 358, 970
BseDI CCNNGG 5 cut(s) 415, 430, 498, 968, 969
BseGI GGATG 5 cut(s) 56, 340, 478, 840, 1196
BseLI CCNNNNNNNGG 3 cut(s) 82, 632, 1187
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 2 cut(s) 323, 920
BseRI GAGGAG 3 cut(s) 68, 101, 1173
BseXI GCAGC 9 cut(s) 34, 140, 281, 315, 321, 362, 462, 920, 1024
BseYI CCCAGC 2 cut(s) 830, 839
BsgI GTGCAG 1 cut(s) 280
Bsh1236I CGCG 2 cut(s) 417, 432
BshFI GGCC 4 cut(s) 197, 356, 674, 1142
BshNI GGYRCC 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 599
BsiSI CCGG 6 cut(s) 345, 390, 482, 627, 633, 958
BslI CCNNNNNNNGG 3 cut(s) 82, 632, 1187
BsmAI GTCTC 2 cut(s) 768, 985
BsmBI CGTCTC 1 cut(s) 985
BsnI GGCC 4 cut(s) 197, 356, 674, 1142
Bsp1286I GDGCHC 1 cut(s) 599
Bsp143I GATC 4 cut(s) 367, 1006, 1183, 1201
BspANI GGCC 4 cut(s) 197, 356, 674, 1142
BspCNI CTCAG 1 cut(s) 93
BspFNI CGCG 2 cut(s) 417, 432
BspLI GGNNCC 2 cut(s) 362, 631
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 360
BsrBI CCGCTC 1 cut(s) 123
BsrI ACTGG 2 cut(s) 323, 920
BssECI CCNNGG 5 cut(s) 415, 430, 498, 968, 969
BssMI GATC 4 cut(s) 367, 1006, 1183, 1201
BssNI GRCGYC 2 cut(s) 361, 978
Bst2UI CCWGG 2 cut(s) 358, 970
Bst4CI ACNGT 6 cut(s) 397, 458, 524, 773, 1020, 1085
Bst6I CTCTTC 4 cut(s) 259, 604, 641, 988
BstACI GRCGYC 2 cut(s) 361, 978
BstAPI GCANNNNNTGC 1 cut(s) 399
BstC8I GCNNGC 3 cut(s) 505, 518, 819
BstDEI CTNAG 1 cut(s) 80
BstDSI CCRYGG 2 cut(s) 415, 430
BstF5I GGATG 5 cut(s) 56, 340, 478, 840, 1196
BstFNI CGCG 2 cut(s) 417, 432
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 4 cut(s) 17, 363, 404, 454
BstKTI GATC 4 cut(s) 370, 1009, 1186, 1204
BstMAI GTCTC 2 cut(s) 768, 985
BstMBI GATC 4 cut(s) 367, 1006, 1183, 1201
BstMWI GCNNNNNNNGC 8 cut(s) 300, 303, 309, 399, 426, 438, 513, 827
BstNI CCWGG 2 cut(s) 358, 970
BstNSI RCATGY 4 cut(s) 163, 274, 507, 821
BstSCI CCNGG 4 cut(s) 343, 356, 625, 968
BstSFI CTRYAG 1 cut(s) 126
BstUI CGCG 2 cut(s) 417, 432
BstV1I GCAGC 9 cut(s) 34, 140, 281, 315, 321, 362, 462, 920, 1024
BstV2I GAAGAC 1 cut(s) 1114
Bsu36I CCTNAGG 1 cut(s) 80
BsuRI GGCC 4 cut(s) 197, 356, 674, 1142
BtgI CCRYGG 2 cut(s) 415, 430
BtsCI GGATG 5 cut(s) 56, 340, 478, 840, 1196
BtsI GCAGTG 1 cut(s) 694
BtsIMutI CAGTG 3 cut(s) 520, 694, 1143
Cac8I GCNNGC 3 cut(s) 505, 518, 819
CfoI GCGC 4 cut(s) 17, 363, 404, 454
Cfr13I GGNCC 2 cut(s) 286, 321
Cfr42I CCGCGG 2 cut(s) 418, 433
Csp6I GTAC 4 cut(s) 163, 274, 490, 658
CviAII CATG 5 cut(s) 160, 271, 504, 818, 901
CviQI GTAC 4 cut(s) 163, 274, 490, 658
DdeI CTNAG 1 cut(s) 80
DinI GGCGCC 1 cut(s) 362
DpnI GATC 4 cut(s) 369, 1008, 1185, 1203
DpnII GATC 4 cut(s) 367, 1006, 1183, 1201
DrdI GACNNNNNNGTC 1 cut(s) 197
DriI GACNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 4 cut(s) 259, 604, 641, 988
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 4 cut(s) 259, 604, 641, 988
EciI GGCGGA 1 cut(s) 278
Ecl136II GAGCTC 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 599
Eco47I GGWCC 2 cut(s) 286, 321
Eco53kI GAGCTC 1 cut(s) 597
Eco57I CTGAAG 1 cut(s) 1023
Eco81I CCTNAGG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 597
EcoRII CCWGG 2 cut(s) 356, 968
EcoT22I ATGCAT 1 cut(s) 819
EcoT38I GRGCYC 1 cut(s) 599
EgeI GGCGCC 1 cut(s) 362
EheI GGCGCC 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 985
FaeI CATG 5 cut(s) 163, 274, 507, 821, 904
FalI AAGNNNNNCTT 4 cut(s) 776, 808, 840, 872
FatI CATG 5 cut(s) 159, 270, 503, 817, 900
FauI CCCGC 5 cut(s) 125, 308, 380, 413, 794
FokI GGATG 5 cut(s) 43, 327, 465, 847, 1203
FriOI GRGCYC 1 cut(s) 599
FspBI CTAG 1 cut(s) 798
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 4 cut(s) 16, 362, 403, 453
GsaI CCCAGC 2 cut(s) 834, 843
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 4 cut(s) 197, 356, 674, 1142
HapII CCGG 6 cut(s) 345, 390, 482, 627, 633, 958
HhaI GCGC 4 cut(s) 17, 363, 404, 454
Hin1I GRCGYC 2 cut(s) 361, 978
Hin1II CATG 5 cut(s) 163, 274, 507, 821, 904
Hin6I GCGC 4 cut(s) 15, 361, 402, 452
HinP1I GCGC 4 cut(s) 15, 361, 402, 452
HincII GTYRAC 4 cut(s) 91, 895, 976, 1198
HindII GTYRAC 4 cut(s) 91, 895, 976, 1198
HindIII AAGCTT 1 cut(s) 1114
HinfI GANTC 4 cut(s) 167, 583, 691, 779
HpaII CCGG 6 cut(s) 345, 390, 482, 627, 633, 958
HphI GGTGA 7 cut(s) 106, 125, 594, 785, 921, 1023, 1073
Hpy166II GTNNAC 4 cut(s) 91, 895, 976, 1198
Hpy188I TCNGA 5 cut(s) 190, 229, 259, 813, 862
Hpy188III TCNNGA 1 cut(s) 1095
Hpy8I GTNNAC 4 cut(s) 91, 895, 976, 1198
Hpy99I CGWCG 3 cut(s) 194, 236, 245
HpyAV CCTTC 1 cut(s) 526
HpyCH4III ACNGT 6 cut(s) 397, 458, 524, 773, 1020, 1085
HpyCH4IV ACGT 1 cut(s) 978
HpyCH4V TGCA 7 cut(s) 128, 297, 478, 507, 642, 817, 947
HpyF10VI GCNNNNNNNGC 8 cut(s) 300, 303, 309, 399, 426, 438, 513, 827
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 1 cut(s) 978
Hsp92I GRCGYC 2 cut(s) 361, 978
Hsp92II CATG 5 cut(s) 163, 274, 507, 821, 904
HspAI GCGC 4 cut(s) 15, 361, 402, 452
KasI GGCGCC 1 cut(s) 360
KspI CCGCGG 2 cut(s) 418, 433
Kzo9I GATC 4 cut(s) 367, 1006, 1183, 1201
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 4 cut(s) 114, 570, 594, 635
Lsp1109I GCAGC 9 cut(s) 34, 140, 281, 315, 321, 362, 462, 920, 1024
LweI GCATC 5 cut(s) 487, 579, 804, 825, 956
MaeI CTAG 1 cut(s) 798
MaeII ACGT 1 cut(s) 978
MaeIII GTNAC 4 cut(s) 718, 788, 953, 1079
MalI GATC 4 cut(s) 369, 1008, 1185, 1203
MbiI CCGCTC 1 cut(s) 123
MboI GATC 4 cut(s) 367, 1006, 1183, 1201
MboII GAAGA 6 cut(s) 246, 591, 658, 975, 1016, 1114
MhlI GDGCHC 1 cut(s) 599
MluCI AATT 2 cut(s) 1036, 1069
Mly113I GGCGCC 1 cut(s) 361
MlyI GAGTC 2 cut(s) 161, 685
MmeI TCCRAC 2 cut(s) 213, 282
Mph1103I ATGCAT 1 cut(s) 819
MspA1I CMGCKG 7 cut(s) 294, 375, 417, 432, 441, 830, 1061
MspI CCGG 6 cut(s) 345, 390, 482, 627, 633, 958
MspR9I CCNGG 4 cut(s) 345, 358, 627, 970
MvaI CCWGG 2 cut(s) 358, 970
MvnI CGCG 2 cut(s) 417, 432
MwoI GCNNNNNNNGC 8 cut(s) 300, 303, 309, 399, 426, 438, 513, 827
NarI GGCGCC 1 cut(s) 361
NciI CCSGG 2 cut(s) 345, 627
NdeII GATC 4 cut(s) 367, 1006, 1183, 1201
NlaIII CATG 5 cut(s) 163, 274, 507, 821, 904
NlaIV GGNNCC 2 cut(s) 362, 631
NmeAIII GCCGAG 3 cut(s) 43, 650, 708
NmuCI GTSAC 3 cut(s) 718, 788, 1079
NsbI TGCGCA 1 cut(s) 453
NsiI ATGCAT 1 cut(s) 819
NspI RCATGY 4 cut(s) 163, 274, 507, 821
PaeI GCATGC 2 cut(s) 507, 821
PasI CCCWGGG 1 cut(s) 969
PciI ACATGT 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 604
PcsI WCGNNNNNNNCGW 5 cut(s) 237, 240, 321, 462, 686
PfeI GAWTC 2 cut(s) 583, 779
PleI GAGTC 2 cut(s) 161, 685
PluTI GGCGCC 1 cut(s) 364
PpsI GAGTC 2 cut(s) 161, 685
PscI ACATGT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 356, 968
PspFI CCCAGC 2 cut(s) 830, 839
PspGI CCWGG 2 cut(s) 356, 968
PspN4I GGNNCC 2 cut(s) 362, 631
PspPI GGNCC 2 cut(s) 286, 321
PstI CTGCAG 1 cut(s) 130
PvuII CAGCTG 3 cut(s) 441, 830, 1061
RsaI GTAC 4 cut(s) 164, 275, 491, 659
RsaNI GTAC 4 cut(s) 163, 274, 490, 658
SacI GAGCTC 1 cut(s) 599
SacII CCGCGG 2 cut(s) 418, 433
SapI GCTCTTC 1 cut(s) 604
Sau3AI GATC 4 cut(s) 367, 1006, 1183, 1201
Sau96I GGNCC 2 cut(s) 286, 321
SchI GAGTC 2 cut(s) 161, 685
ScrFI CCNGG 4 cut(s) 345, 358, 627, 970
SduI GDGCHC 1 cut(s) 599
SfaNI GCATC 5 cut(s) 487, 579, 804, 825, 956
SfcI CTRYAG 1 cut(s) 126
SfoI GGCGCC 1 cut(s) 362
Sfr303I CCGCGG 2 cut(s) 418, 433
SgrBI CCGCGG 2 cut(s) 418, 433
SinI GGWCC 2 cut(s) 286, 321
SmlI CTYRAG 1 cut(s) 1105
SmoI CTYRAG 1 cut(s) 1105
SphI GCATGC 2 cut(s) 507, 821
Sse9I AATT 2 cut(s) 1036, 1069
SspDI GGCGCC 1 cut(s) 360
SspMI CTAG 1 cut(s) 798
SstI GAGCTC 1 cut(s) 599
StyD4I CCNGG 4 cut(s) 343, 356, 625, 968
TaaI ACNGT 6 cut(s) 397, 458, 524, 773, 1020, 1085
TaiI ACGT 1 cut(s) 981
TaqI TCGA 3 cut(s) 465, 689, 1125
TaqII GACCGA 1 cut(s) 338
TasI AATT 2 cut(s) 1036, 1069
TauI GCSGC 7 cut(s) 123, 294, 309, 417, 420, 432, 435
TfiI GAWTC 2 cut(s) 583, 779
TscAI CASTG 3 cut(s) 527, 701, 1150
TseFI GTSAC 3 cut(s) 718, 788, 1079
TseI GCWGC 9 cut(s) 22, 128, 294, 303, 309, 375, 475, 908, 1012
Tsp45I GTSAC 3 cut(s) 718, 788, 1079
TspDTI ATGAA 7 cut(s) 45, 624, 792, 902, 915, 975, 1012
TspGWI ACGGA 1 cut(s) 607
TspRI CASTG 3 cut(s) 527, 701, 1150
VpaK11BI GGWCC 2 cut(s) 286, 321
XceI RCATGY 4 cut(s) 163, 274, 507, 821
XspI CTAG 1 cut(s) 798
ZraI GACGTC 1 cut(s) 979
Zsp2I ATGCAT 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.