FvH4_5g18540

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004447
Physical Location & Seq
Forward (+)
10668596 .. 10675200
6605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18540.t3

Sequence Viewer

Length: 1278 bp
ATGGGATGCCTGGTGTCAACACCGAAAGATACAGGTGGAAATAGGAGGAGGCCATCAAACATTGGTGAAGTTTCTGTTTATGTTCCTGGTCTGCGAATACCTAAACCTGTCGATTTCTCTCACTCACTTGGTGATCACTTGTCCAAGAGCTTAGTAGAACGTCTATCTGCTTTAAGAACTCGTATAGTTGTGATGGCTGGCCAAGAAGGTCCTACAATCACGAGAACAAGGAGAAGAACCCAACACGGTGGTTCAACATTAGCTGATCTCCTGCAGGCTCTCGAGGATTACTTACCTGTTCTTTTGGGATTAATGAAAGATGGAAGCCAGCTACAACACAAGGTACAATTTGTGTGGGTAAATCAAGAGGATGATGCAGAGGAAACAACTATGTCTAATGCATGGTACGAGGTGCTGTCAGTTTTGCATTTAATGGCAATGCTCTCATTATCACAAGCTAACTTATTACTTCTTCCGAGAACATCTACTGACGGTCACCAGCCAAAAGTATCAGAAGAGAGTAGACGGGCTTCGGTTGATATATTCCTGAAGGCAGCAGGACACCTTGACTGTGCTGTCCGGCATGTTCTCCCACAGTTGCCTAATGAGCTCAAGAGAAACCTACCAGTCGACCTAGCAGAAGGAGTTCTTCGAGCTCTTTGCTTACAAGCACTAGGACAGGGTGTTGATATTCAACTTGGAATGGCAATTGATAGTACAAAAGCCACTCTTGCAGTGAAGCGAAGGCTTGCATGTGAGATGGTAAAGTATTGGCAGCAGGCTCAAGATAACATTATGAATCTTCCATTATTAAATGGTTGGGGTGAGAAGCATAAACTCTTTGTAAAGTGGAAATATGCTGAGGCAAAGGCTGGTGCATATTATTATCATGGTTTAATCCTCGATGAGGGTAACACAGAAAAATTTCATGGAATGGCAGTAGCTGCTTTGCAAGCAGCAGACGAGTATTTTAAAGAAAGTAAGAAGGCATGCGAGGCATTTAATGCTGCTCCCCCTTTGTCCAGAAACCCACCGCTTTGGGGAACCATGAAATATCTATCTGAGAAAATCCCAAAAGATACTTCAAGCAAAGTGCGGATAAACCGTGATTTGTACTCTTATCAAAAAATCATGGAGACGGCACCGACATTGCCCGACTTTGCTCTGGCCTTGAAACCCGACGAATATCAACTCCCTTCGGTGGATCCCTCTTGGAACATGGAGCAAACAAATAGGGGAGCTACTGCTCCCAACCAACTCAAGAATGACAGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

426

Amino Acids

47.42

Weight (kDa)

8.76

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 83 - 330 6.9e-14 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 1141
AccI GTMKAC 2 cut(s) 523, 630
AciI CCGC 2 cut(s) 1034, 1096
AclWI GGATC 2 cut(s) 1199, 1212
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 1 cut(s) 923
AcuI CTGAAG 1 cut(s) 569
AdeI CACNNNGTG 1 cut(s) 131
AfaI GTAC 4 cut(s) 345, 407, 718, 1115
AfiI CCNNNNNNNGG 3 cut(s) 907, 1040, 1201
AgsI TTSAA 4 cut(s) 255, 695, 1086, 1174
AjnI CCWGG 2 cut(s) 9, 85
AjuI GAANNNNNNNTTGG 2 cut(s) 1066, 1098
AluBI AGCT 8 cut(s) 150, 263, 331, 458, 610, 656, 944, 1241
AluI AGCT 8 cut(s) 150, 263, 331, 458, 610, 656, 944, 1241
Alw21I GWGCWC 2 cut(s) 612, 658
Alw26I GTCTC 1 cut(s) 1130
AlwI GGATC 2 cut(s) 1199, 1212
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 3 cut(s) 50, 199, 1167
ApeKI GCWGC 5 cut(s) 554, 775, 944, 956, 1007
ApoI RAATTY 1 cut(s) 923
AseI ATTAAT 1 cut(s) 311
Asp700I GAANNNNTTC 2 cut(s) 645, 924
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 4 cut(s) 77, 143, 488, 836
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 201
BamHI GGATCC 1 cut(s) 1204
BanI GGYRCC 1 cut(s) 1141
BanII GRGCYC 2 cut(s) 612, 658
BarI GAAGNNNNNNTAC 2 cut(s) 514, 546
BauI CACGAG 1 cut(s) 220
Bbv12I GWGCWC 2 cut(s) 612, 658
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 5 cut(s) 566, 787, 931, 968, 994
BccI CCATC 4 cut(s) 61, 187, 314, 754
BceAI ACGGC 1 cut(s) 1155
BcgI CGANNNNNNTGC 2 cut(s) 642, 676
BciT130I CCWGG 2 cut(s) 11, 87
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 1130
BfaI CTAG 2 cut(s) 635, 674
BfmI CTRYAG 1 cut(s) 272
BisI GCNGC 5 cut(s) 555, 776, 945, 957, 1008
BlsI GCNGC 5 cut(s) 556, 777, 946, 958, 1009
Bme1390I CCNGG 2 cut(s) 11, 87
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 3 cut(s) 1045, 1143, 1206
BmrFI CCNGG 2 cut(s) 11, 87
BmsI GCATC 1 cut(s) 364
Bpu10I CCTNAGC 1 cut(s) 861
BpuEI CTTGAG 3 cut(s) 596, 768, 1244
Bsc4I CCNNNNNNNGG 3 cut(s) 907, 1040, 1201
Bse1I ACTGG 1 cut(s) 626
Bse3DI GCAATG 2 cut(s) 444, 1148
BseBI CCWGG 2 cut(s) 11, 87
BseGI GGATG 2 cut(s) 11, 376
BseLI CCNNNNNNNGG 3 cut(s) 907, 1040, 1201
BseMI GCAATG 2 cut(s) 444, 1148
BseMII CTCAG 2 cut(s) 852, 1053
BseNI ACTGG 1 cut(s) 626
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 5 cut(s) 566, 787, 931, 968, 994
BshFI GGCC 3 cut(s) 52, 201, 1169
BshNI GGYRCC 1 cut(s) 1141
BsiHKAI GWGCWC 2 cut(s) 612, 658
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 1 cut(s) 580
BslI CCNNNNNNNGG 3 cut(s) 907, 1040, 1201
BsmAI GTCTC 1 cut(s) 1130
BsmBI CGTCTC 1 cut(s) 1130
BsnI GGCC 3 cut(s) 52, 201, 1169
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 2 cut(s) 612, 658
Bsp143I GATC 3 cut(s) 133, 265, 1204
BspACI CCGC 2 cut(s) 1034, 1096
BspANI GGCC 3 cut(s) 52, 201, 1169
BspCNI CTCAG 2 cut(s) 853, 1054
BspLI GGNNCC 3 cut(s) 1045, 1143, 1206
BspMAI CTGCAG 1 cut(s) 276
BspPI GGATC 2 cut(s) 1199, 1212
BspT107I GGYRCC 1 cut(s) 1141
BsrDI GCAATG 2 cut(s) 444, 1148
BsrI ACTGG 1 cut(s) 626
BssMI GATC 3 cut(s) 133, 265, 1204
BssSI CACGAG 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 220
Bst2UI CCWGG 2 cut(s) 11, 87
Bst4CI ACNGT 5 cut(s) 248, 494, 572, 597, 1106
Bst6I CTCTTC 1 cut(s) 510
BstAPI GCANNNNNTGC 2 cut(s) 944, 1004
BstC8I GCNNGC 7 cut(s) 199, 276, 329, 750, 780, 954, 991
BstDEI CTNAG 3 cut(s) 151, 861, 1062
BstEII GGTNACC 1 cut(s) 494
BstENI CCTNNNNNAGG 1 cut(s) 905
BstF5I GGATG 2 cut(s) 11, 376
BstKTI GATC 3 cut(s) 136, 268, 1207
BstMAI GTCTC 1 cut(s) 1130
BstMBI GATC 3 cut(s) 133, 265, 1204
BstMWI GCNNNNNNNGC 6 cut(s) 607, 731, 944, 953, 995, 1004
BstNI CCWGG 2 cut(s) 11, 87
BstNSI RCATGY 3 cut(s) 587, 756, 993
BstPI GGTNACC 1 cut(s) 494
BstSCI CCNGG 2 cut(s) 9, 85
BstSFI CTRYAG 1 cut(s) 272
BstV1I GCAGC 5 cut(s) 566, 787, 931, 968, 994
BstX2I RGATCY 1 cut(s) 1204
BstXI CCANNNNNNTGG 2 cut(s) 248, 1038
BstYI RGATCY 1 cut(s) 1204
BsuRI GGCC 3 cut(s) 52, 201, 1169
BtsCI GGATG 2 cut(s) 11, 376
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 741
Cac8I GCNNGC 7 cut(s) 199, 276, 329, 750, 780, 954, 991
CaiI CAGNNNCTG 1 cut(s) 944
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 4 cut(s) 344, 406, 717, 1114
CspCI CAANNNNNGTGG 2 cut(s) 335, 370
CviAII CATG 9 cut(s) 402, 584, 753, 890, 929, 990, 1048, 1132, 1219
CviQI GTAC 4 cut(s) 344, 406, 717, 1114
DdeI CTNAG 3 cut(s) 151, 861, 1062
DpnI GATC 3 cut(s) 135, 267, 1206
DpnII GATC 3 cut(s) 133, 265, 1204
DraI TTTAAA 1 cut(s) 973
DraIII CACNNNGTG 1 cut(s) 131
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Ecl136II GAGCTC 2 cut(s) 610, 656
Eco24I GRGCYC 2 cut(s) 612, 658
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 2 cut(s) 610, 656
Eco57I CTGAAG 1 cut(s) 569
Eco88I CYCGRG 1 cut(s) 281
Eco91I GGTNACC 1 cut(s) 494
EcoICRI GAGCTC 2 cut(s) 610, 656
EcoNI CCTNNNNNAGG 1 cut(s) 905
EcoO109I RGGNCCY 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 494
EcoRII CCWGG 2 cut(s) 9, 85
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 2 cut(s) 612, 658
Esp3I CGTCTC 1 cut(s) 1130
FaeI CATG 9 cut(s) 405, 587, 756, 893, 932, 993, 1051, 1135, 1222
FalI AAGNNNNNCTT 4 cut(s) 633, 665, 714, 746
FatI CATG 9 cut(s) 401, 583, 752, 889, 928, 989, 1047, 1131, 1218
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 2 cut(s) 523, 630
Fnu4HI GCNGC 5 cut(s) 555, 776, 945, 957, 1008
FokI GGATG 2 cut(s) 18, 383
FriOI GRGCYC 2 cut(s) 612, 658
Fsp4HI GCNGC 5 cut(s) 555, 776, 945, 957, 1008
FspBI CTAG 2 cut(s) 635, 674
GluI GCNGC 5 cut(s) 555, 776, 945, 957, 1008
HaeIII GGCC 3 cut(s) 52, 201, 1169
HapII CCGG 1 cut(s) 580
Hin1II CATG 9 cut(s) 405, 587, 756, 893, 932, 993, 1051, 1135, 1222
HincII GTYRAC 2 cut(s) 18, 631
HindII GTYRAC 2 cut(s) 18, 631
HinfI GANTC 1 cut(s) 799
HpaII CCGG 1 cut(s) 580
HphI GGTGA 4 cut(s) 77, 143, 488, 836
Hpy166II GTNNAC 3 cut(s) 18, 524, 631
Hpy188I TCNGA 3 cut(s) 477, 514, 1063
Hpy188III TCNNGA 8 cut(s) 220, 281, 365, 547, 613, 785, 1023, 1261
Hpy8I GTNNAC 3 cut(s) 18, 524, 631
Hpy99I CGWCG 1 cut(s) 1184
HpyAV CCTTC 6 cut(s) 200, 544, 635, 738, 979, 1206
HpyCH4III ACNGT 5 cut(s) 248, 494, 572, 597, 1106
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 8 cut(s) 274, 377, 401, 427, 734, 752, 878, 952
HpyF10VI GCNNNNNNNGC 6 cut(s) 607, 731, 944, 953, 995, 1004
HpyF3I CTNAG 3 cut(s) 151, 861, 1062
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 9 cut(s) 405, 587, 756, 893, 932, 993, 1051, 1135, 1222
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 133, 265, 1204
LmnI GCTCC 4 cut(s) 1015, 1222, 1238, 1252
Lsp1109I GCAGC 5 cut(s) 566, 787, 931, 968, 994
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 635, 674
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 494, 911
MalI GATC 3 cut(s) 135, 267, 1206
MboI GATC 3 cut(s) 133, 265, 1204
MboII GAAGA 5 cut(s) 246, 464, 527, 641, 794
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 1 cut(s) 1204
MhlI GDGCHC 2 cut(s) 612, 658
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 3 cut(s) 347, 708, 923
MluNI TGGCCA 1 cut(s) 201
Mox20I TGGCCA 1 cut(s) 201
Mph1103I ATGCAT 1 cut(s) 403
MroXI GAANNNNTTC 2 cut(s) 645, 924
MscI TGGCCA 1 cut(s) 201
MseI TTAA 7 cut(s) 173, 311, 431, 812, 896, 972, 1002
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 1 cut(s) 580
MspR9I CCNGG 2 cut(s) 11, 87
MunI CAATTG 1 cut(s) 708
MvaI CCWGG 2 cut(s) 11, 87
MwoI GCNNNNNNNGC 6 cut(s) 607, 731, 944, 953, 995, 1004
NdeII GATC 3 cut(s) 133, 265, 1204
NlaIII CATG 9 cut(s) 405, 587, 756, 893, 932, 993, 1051, 1135, 1222
NlaIV GGNNCC 3 cut(s) 1045, 1143, 1206
NmuCI GTSAC 1 cut(s) 494
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 3 cut(s) 587, 756, 993
PaeI GCATGC 1 cut(s) 993
PaeR7I CTCGAG 1 cut(s) 281
PdmI GAANNNNTTC 2 cut(s) 645, 924
PfeI GAWTC 1 cut(s) 799
PkrI GCNGC 5 cut(s) 556, 777, 946, 958, 1009
PpuMI RGGWCCY 1 cut(s) 209
PshBI ATTAAT 1 cut(s) 311
Psp124BI GAGCTC 2 cut(s) 612, 658
Psp5II RGGWCCY 1 cut(s) 209
Psp6I CCWGG 2 cut(s) 9, 85
PspEI GGTNACC 1 cut(s) 494
PspGI CCWGG 2 cut(s) 9, 85
PspN4I GGNNCC 3 cut(s) 1045, 1143, 1206
PspPI GGNCC 1 cut(s) 209
PspPPI RGGWCCY 1 cut(s) 209
PstI CTGCAG 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 1 cut(s) 1204
RsaI GTAC 4 cut(s) 345, 407, 718, 1115
RsaNI GTAC 4 cut(s) 344, 406, 717, 1114
SacI GAGCTC 2 cut(s) 612, 658
SalI GTCGAC 1 cut(s) 629
SaqAI TTAA 7 cut(s) 173, 311, 431, 812, 896, 972, 1002
SatI GCNGC 5 cut(s) 555, 776, 945, 957, 1008
Sau3AI GATC 3 cut(s) 133, 265, 1204
Sau96I GGNCC 1 cut(s) 209
SbfI CCTGCAGG 1 cut(s) 276
ScrFI CCNGG 2 cut(s) 11, 87
SdaI CCTGCAGG 1 cut(s) 276
SduI GDGCHC 2 cut(s) 612, 658
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 1 cut(s) 272
Sfr274I CTCGAG 1 cut(s) 281
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 281
SmlI CTYRAG 4 cut(s) 281, 611, 783, 1259
SmoI CTYRAG 4 cut(s) 281, 611, 783, 1259
SphI GCATGC 1 cut(s) 993
Sse8387I CCTGCAGG 1 cut(s) 276
Sse9I AATT 3 cut(s) 347, 708, 923
SsiI CCGC 2 cut(s) 1034, 1096
SspMI CTAG 2 cut(s) 635, 674
SstI GAGCTC 2 cut(s) 612, 658
StyD4I CCNGG 2 cut(s) 9, 85
TaaI ACNGT 5 cut(s) 248, 494, 572, 597, 1106
TaiI ACGT 1 cut(s) 163
TaqI TCGA 5 cut(s) 111, 282, 630, 652, 903
TasI AATT 3 cut(s) 347, 708, 923
TatI WGTACW 2 cut(s) 716, 1113
TfiI GAWTC 1 cut(s) 799
Tru1I TTAA 7 cut(s) 173, 311, 431, 812, 896, 972, 1002
Tru9I TTAA 7 cut(s) 173, 311, 431, 812, 896, 972, 1002
TscAI CASTG 1 cut(s) 741
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 5 cut(s) 554, 775, 944, 956, 1007
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 4 cut(s) 329, 812, 917, 1064
TspRI CASTG 1 cut(s) 741
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 311
XagI CCTNNNNNAGG 1 cut(s) 905
XapI RAATTY 1 cut(s) 923
XceI RCATGY 3 cut(s) 587, 756, 993
XhoI CTCGAG 1 cut(s) 281
XmiI GTMKAC 2 cut(s) 523, 630
XmnI GAANNNNTTC 2 cut(s) 645, 924
XspI CTAG 2 cut(s) 635, 674
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.