MD14G1246500.v1.1

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004447
Physical Location & Seq
Forward (+)
32424876 .. 32431502
6627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1246500.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGGGATGCCTGGTCTCAACACCGAAAGATACACTAGGAAATAGGAGGAGGCCGGCAAACATTGGGGAAGTTTCTGTCTATGTTCCTGGTTTGCGAATCCCTAAACCTGTTGATTTCTCTCAGTCACTTGGTGATCACTTGTCCAAAAGTTTAGTGGAACGCCTATCTGCTTTAAGGACTCGCATAGTTGTAATGGCTGCCCAAGAAGGTCCAACAATTACCAGAACGAGGAGAAGAGCTGCTACTCAACATGGTGGTTCAACATTGGCCGATCTTCAGCAGGCTCTCGAGGATTATTTGCCTGTCCTTTTGGGATTAATGAAAGATGGAAGCCAGCTACACCACAAGGTTCAATTTTTGTGGGTAAATCATGAGGATGATGCAGAGGAAACAGCTATGTCTAATGCATGGTACGAGGTGCTGTCGATTTTGCACTTAATGGCAATGCTATCATTATCACAAGCTAACTTGTTACTTCTTCCAAGAACATCTGGTGATGGTTATCAGCCAAAAGTAACAGAAGAGAGTAGGCGAGCTTCCATTGATATTTTCCTGAAGGCAGCAGGATACCTTGATTGTGCCGTCCGACATGTTCTTCCACAGTTGCCTACTGAGCTCAAGAGAAACCTGCCAATTGACTTAGCAGAAGGAGTTCTTCGAGCGCTTTGCCTACAAGCATTAGGACAGGGTGTTGATATTCAACTTGGAATGGCAATAGATAGCACAAAAGCTACACTTGCAGTGAAGCGAAGGCTTGCATGTGAAATGGTGAAGTATTGGCAACAGGCTCAAGATAACATTATGAACCTTCCATTATTAAATGGTTGGGGTGAAAAGCATCGGCTCTTTGTGAAGTGGAAATATGTTGAGGCAAAGGCTGCTGCATATTACTATCATGGTTTGATCCTTGATGAGGGTAACACAGAAAAATTTCATGGGATGGCTGTAGCTGCTTTGCAAGCAGCAGATGAGTATTTCAAAGAAAGTAAGAAGGCATGCGAAGCATTTAATGCTGCTCCCCCTTTGTCGAGAAATCCACCGCTCTGGGGAACCATGAAGTATCTATCTGAGAAAATTCCAAAAGATGCTTCAAGCAAAGTGCGGATAAACCGTGATCTGTACTCACACGAGAAAATCATGGAGACAGCACCAACATTGCCCGATTTTGCATTGGCCTTGAAACCGGATGAATTCCAACTTCCTCCAGTAGACCCCTCTTGGAACATGGAGCACATGAATAAGGGCTCCAACCAGCTCAGGAATGACAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

425

Amino Acids

47.46

Weight (kDa)

8.43

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 70 - 332 8.1e-14 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 636
AccBSI CCGCTC 1 cut(s) 1042
AccI GTMKAC 1 cut(s) 1209
AciI CCGC 2 cut(s) 1040, 1102
AclWI GGATC 1 cut(s) 898
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 3 cut(s) 929, 1074, 1190
AcuI CTGAAG 2 cut(s) 260, 575
AdeI CACNNNGTG 1 cut(s) 131
AfaI GTAC 2 cut(s) 413, 1121
AfeI AGCGCT 1 cut(s) 663
AfiI CCNNNNNNNGG 3 cut(s) 228, 913, 1046
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 6 cut(s) 261, 353, 701, 979, 1092, 1180
AjnI CCWGG 2 cut(s) 9, 85
AjuI GAANNNNNNNTTGG 2 cut(s) 1072, 1104
AluBI AGCT 9 cut(s) 239, 337, 395, 464, 536, 616, 731, 950, 1255
AluI AGCT 9 cut(s) 239, 337, 395, 464, 536, 616, 731, 950, 1255
Alw21I GWGCWC 2 cut(s) 618, 1233
Alw26I GTCTC 2 cut(s) 19, 1136
AlwI GGATC 1 cut(s) 898
Ama87I CYCGRG 1 cut(s) 287
Aor51HI AGCGCT 1 cut(s) 663
AoxI GGCC 3 cut(s) 50, 267, 1173
ApeKI GCWGC 8 cut(s) 197, 239, 560, 878, 881, 950, 962, 1013
ApoI RAATTY 3 cut(s) 929, 1074, 1190
AseI ATTAAT 1 cut(s) 317
Asp700I GAANNNNTTC 2 cut(s) 651, 930
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 4 cut(s) 143, 506, 781, 842
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 209
BanII GRGCYC 2 cut(s) 618, 1247
BarI GAAGNNNNNNTAC 4 cut(s) 226, 258, 520, 552
BauI CACGAG 1 cut(s) 1127
Bbv12I GWGCWC 2 cut(s) 618, 1233
BbvI GCAGC 8 cut(s) 184, 226, 572, 865, 868, 937, 974, 1000
BccI CCATC 3 cut(s) 320, 491, 934
BceAI ACGGC 1 cut(s) 566
BcgI CGANNNNNNTGC 2 cut(s) 648, 682
BciT130I CCWGG 2 cut(s) 11, 87
BciVI GTATCC 1 cut(s) 560
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 19, 1136
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 1 cut(s) 945
BfoI RGCGCY 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 636
BfuI GTATCC 1 cut(s) 560
BisI GCNGC 8 cut(s) 198, 240, 561, 879, 882, 951, 963, 1014
BlsI GCNGC 8 cut(s) 199, 241, 562, 880, 883, 952, 964, 1015
Bme1390I CCNGG 2 cut(s) 11, 87
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 1051, 1246
BmrFI CCNGG 2 cut(s) 11, 87
BmsI GCATC 3 cut(s) 370, 847, 1075
BpmI CTGGAG 1 cut(s) 1188
Bpu10I CCTNAGC 1 cut(s) 1256
BpuEI CTTGAG 2 cut(s) 602, 774
BsaBI GATNNNNATC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 19
BsaWI WCCGGW 1 cut(s) 1183
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 913, 1046
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 1 cut(s) 1205
Bse3DI GCAATG 2 cut(s) 450, 1154
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 2 cut(s) 11, 87
BseGI GGATG 4 cut(s) 11, 382, 945, 1192
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 3 cut(s) 228, 913, 1046
BseMI GCAATG 2 cut(s) 450, 1154
BseMII CTCAG 4 cut(s) 134, 603, 1059, 1270
BseNI ACTGG 1 cut(s) 1205
BseRI GAGGAG 2 cut(s) 61, 244
BseXI GCAGC 8 cut(s) 184, 226, 572, 865, 868, 937, 974, 1000
BshFI GGCC 3 cut(s) 52, 269, 1175
BsiHKAI GWGCWC 2 cut(s) 618, 1233
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 53, 1184
BslI CCNNNNNNNGG 3 cut(s) 228, 913, 1046
BsmAI GTCTC 2 cut(s) 19, 1136
BsnI GGCC 3 cut(s) 52, 269, 1175
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 618, 1233, 1247
Bsp143I GATC 4 cut(s) 133, 271, 903, 1114
BspACI CCGC 2 cut(s) 1040, 1102
BspANI GGCC 3 cut(s) 52, 269, 1175
BspCNI CTCAG 4 cut(s) 133, 604, 1060, 1269
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 1051, 1246
BspMI ACCTGC 1 cut(s) 636
BspPI GGATC 1 cut(s) 898
BspQI GCTCTTC 1 cut(s) 229
BspTNI GGTCTC 1 cut(s) 19
BsrBI CCGCTC 1 cut(s) 1042
BsrDI GCAATG 2 cut(s) 450, 1154
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 1 cut(s) 1205
BssAI RCCGGY 1 cut(s) 52
BssMI GATC 4 cut(s) 133, 271, 903, 1114
BssSI CACGAG 1 cut(s) 1127
Bst2BI CACGAG 1 cut(s) 1127
Bst2UI CCWGG 2 cut(s) 11, 87
Bst4CI ACNGT 2 cut(s) 603, 1112
Bst6I CTCTTC 2 cut(s) 229, 516
BstAPI GCANNNNNTGC 2 cut(s) 878, 1010
BstC8I GCNNGC 7 cut(s) 54, 282, 335, 534, 756, 960, 997
BstDEI CTNAG 5 cut(s) 120, 612, 640, 1068, 1256
BstENI CCTNNNNNAGG 1 cut(s) 911
BstF5I GGATG 4 cut(s) 11, 382, 945, 1192
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 4 cut(s) 136, 274, 906, 1117
BstMAI GTCTC 2 cut(s) 19, 1136
BstMBI GATC 4 cut(s) 133, 271, 903, 1114
BstMWI GCNNNNNNNGC 7 cut(s) 613, 737, 878, 950, 959, 1001, 1010
BstNI CCWGG 2 cut(s) 11, 87
BstNSI RCATGY 3 cut(s) 593, 762, 999
BstSCI CCNGG 2 cut(s) 9, 85
BstSFI CTRYAG 1 cut(s) 945
BstV1I GCAGC 8 cut(s) 184, 226, 572, 865, 868, 937, 974, 1000
BstXI CCANNNNNNTGG 1 cut(s) 1044
BsuI GTATCC 1 cut(s) 560
BsuRI GGCC 3 cut(s) 52, 269, 1175
BtsCI GGATG 4 cut(s) 11, 382, 945, 1192
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 1 cut(s) 747
BveI ACCTGC 1 cut(s) 636
Cac8I GCNNGC 7 cut(s) 54, 282, 335, 534, 756, 960, 997
CciI TCATGA 1 cut(s) 370
CfoI GCGC 1 cut(s) 664
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 412, 1120
CspCI CAANNNNNGTGG 2 cut(s) 341, 376
CviQI GTAC 2 cut(s) 412, 1120
DdeI CTNAG 5 cut(s) 120, 612, 640, 1068, 1256
DpnI GATC 4 cut(s) 135, 273, 905, 1116
DpnII GATC 4 cut(s) 133, 271, 903, 1114
DraIII CACNNNGTG 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 229, 516
EarI CTCTTC 2 cut(s) 229, 516
Ecl136II GAGCTC 1 cut(s) 616
Eco24I GRGCYC 2 cut(s) 618, 1247
Eco31I GGTCTC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 209
Eco47III AGCGCT 1 cut(s) 663
Eco53kI GAGCTC 1 cut(s) 616
Eco57I CTGAAG 2 cut(s) 260, 575
Eco88I CYCGRG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 616
EcoNI CCTNNNNNAGG 1 cut(s) 911
EcoRI GAATTC 1 cut(s) 1190
EcoRII CCWGG 2 cut(s) 9, 85
EcoT22I ATGCAT 1 cut(s) 409
EcoT38I GRGCYC 2 cut(s) 618, 1247
FalI AAGNNNNNCTT 4 cut(s) 639, 671, 720, 752
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 1 cut(s) 1209
Fnu4HI GCNGC 8 cut(s) 198, 240, 561, 879, 882, 951, 963, 1014
FokI GGATG 4 cut(s) 18, 389, 952, 1199
FriOI GRGCYC 2 cut(s) 618, 1247
Fsp4HI GCNGC 8 cut(s) 198, 240, 561, 879, 882, 951, 963, 1014
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 663
GluI GCNGC 8 cut(s) 198, 240, 561, 879, 882, 951, 963, 1014
GsuI CTGGAG 1 cut(s) 1188
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 3 cut(s) 52, 269, 1175
HapII CCGG 2 cut(s) 53, 1184
HhaI GCGC 1 cut(s) 664
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HinfI GANTC 2 cut(s) 96, 178
HpaII CCGG 2 cut(s) 53, 1184
HphI GGTGA 4 cut(s) 143, 506, 781, 842
Hpy166II GTNNAC 1 cut(s) 1210
Hpy188I TCNGA 2 cut(s) 587, 1069
Hpy188III TCNNGA 7 cut(s) 287, 371, 553, 619, 791, 1029, 1258
Hpy8I GTNNAC 1 cut(s) 1210
HpyAV CCTTC 6 cut(s) 200, 550, 641, 744, 818, 985
HpyCH4III ACNGT 2 cut(s) 603, 1112
HpyCH4V TGCA 8 cut(s) 383, 407, 433, 740, 758, 884, 958, 1169
HpyF10VI GCNNNNNNNGC 7 cut(s) 613, 737, 878, 950, 959, 1001, 1010
HpyF3I CTNAG 5 cut(s) 120, 612, 640, 1068, 1256
HspAI GCGC 1 cut(s) 662
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 4 cut(s) 133, 271, 903, 1114
LguI GCTCTTC 1 cut(s) 229
LmnI GCTCC 3 cut(s) 1021, 1228, 1250
Lsp1109I GCAGC 8 cut(s) 184, 226, 572, 865, 868, 937, 974, 1000
LweI GCATC 3 cut(s) 370, 847, 1075
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 4 cut(s) 123, 471, 514, 917
MalI GATC 4 cut(s) 135, 273, 905, 1116
MbiI CCGCTC 1 cut(s) 1042
MboI GATC 4 cut(s) 133, 271, 903, 1114
MboII GAAGA 6 cut(s) 246, 266, 470, 533, 587, 647
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 3 cut(s) 618, 1233, 1247
MluCI AATT 6 cut(s) 216, 353, 633, 929, 1074, 1190
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 4 cut(s) 236, 610, 1219, 1272
Mph1103I ATGCAT 1 cut(s) 409
MroNI GCCGGC 1 cut(s) 52
MroXI GAANNNNTTC 2 cut(s) 651, 930
MseI TTAA 5 cut(s) 173, 317, 437, 818, 1008
MslI CAYNNNNRTG 1 cut(s) 375
MspI CCGG 2 cut(s) 53, 1184
MspR9I CCNGG 2 cut(s) 11, 87
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 2 cut(s) 11, 87
MwoI GCNNNNNNNGC 7 cut(s) 613, 737, 878, 950, 959, 1001, 1010
NaeI GCCGGC 1 cut(s) 54
NdeII GATC 4 cut(s) 133, 271, 903, 1114
NgoMIV GCCGGC 1 cut(s) 52
NlaIV GGNNCC 2 cut(s) 1051, 1246
NmuCI GTSAC 1 cut(s) 123
NsiI ATGCAT 1 cut(s) 409
NspI RCATGY 3 cut(s) 593, 762, 999
PaeI GCATGC 1 cut(s) 999
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 370
PciI ACATGT 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 229
PdiI GCCGGC 1 cut(s) 54
PdmI GAANNNNTTC 2 cut(s) 651, 930
PfeI GAWTC 1 cut(s) 96
PkrI GCNGC 8 cut(s) 199, 241, 562, 880, 883, 952, 964, 1015
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PscI ACATGT 1 cut(s) 589
PshBI ATTAAT 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 618
Psp6I CCWGG 2 cut(s) 9, 85
PspGI CCWGG 2 cut(s) 9, 85
PspN4I GGNNCC 2 cut(s) 1051, 1246
PspPI GGNCC 1 cut(s) 209
RsaI GTAC 2 cut(s) 413, 1121
RsaNI GTAC 2 cut(s) 412, 1120
RseI CAYNNNNRTG 1 cut(s) 375
SacI GAGCTC 1 cut(s) 618
SapI GCTCTTC 1 cut(s) 229
SaqAI TTAA 5 cut(s) 173, 317, 437, 818, 1008
SatI GCNGC 8 cut(s) 198, 240, 561, 879, 882, 951, 963, 1014
Sau3AI GATC 4 cut(s) 133, 271, 903, 1114
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 2 cut(s) 11, 87
SduI GDGCHC 3 cut(s) 618, 1233, 1247
SfaNI GCATC 3 cut(s) 370, 847, 1075
SfcI CTRYAG 1 cut(s) 945
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 3 cut(s) 287, 617, 789
SmoI CTYRAG 3 cut(s) 287, 617, 789
SphI GCATGC 1 cut(s) 999
Sse9I AATT 6 cut(s) 216, 353, 633, 929, 1074, 1190
SsiI CCGC 2 cut(s) 1040, 1102
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 618
StyD4I CCNGG 2 cut(s) 9, 85
TaaI ACNGT 2 cut(s) 603, 1112
TaqI TCGA 4 cut(s) 288, 425, 658, 1028
TasI AATT 6 cut(s) 216, 353, 633, 929, 1074, 1190
TatI WGTACW 1 cut(s) 1119
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 5 cut(s) 173, 317, 437, 818, 1008
Tru9I TTAA 5 cut(s) 173, 317, 437, 818, 1008
TscAI CASTG 1 cut(s) 747
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 8 cut(s) 197, 239, 560, 878, 881, 950, 962, 1013
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 6 cut(s) 335, 818, 923, 1070, 1203, 1250
TspRI CASTG 1 cut(s) 747
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 317
XagI CCTNNNNNAGG 1 cut(s) 911
XapI RAATTY 3 cut(s) 929, 1074, 1190
XceI RCATGY 3 cut(s) 593, 762, 999
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 1209
XmnI GAANNNNTTC 2 cut(s) 651, 930
XspI CTAG 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.