Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0004447

Physical Location & Seq

Genomic Coordinates Forward (+)

24156504 .. 24161025

Gene Structure 4522 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g21560.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGGATGCCTGGTCTCAACACCGAAAGATACACTAGGAAATAGGAGAAGGCCCGCAAACATTGGGGAAGTCTCTGTCTATGTCCCTGGTTTGCGAATCCCTAAACCTGTTGATTTCTCTCAGTCACTTGGTGATCACTTGTCCAAGAGTTTAGTGGAACGCCTATCTGCATTAAGGACTCGCATAGTTGTGATGGCTGCCCAAGAAGGTCCGACAATTACCAGAACGAAGAGAAGAGCTGCTACTCAACATGGTTTGTGGTTCAACATTGGCCGATCGATCTTTGATTGTATCTTTATTCAGCAGGCTCTCGAGGATTACTTGCCTGTCCTTTTGGGATTAATGAAAGATGGAAGCCAGCTACACCACAAGGTTCAATTTTTGTGGGTAAATCAAGAGGATGATGCAGAGGAAACAGCTATGTCTAACGCATGGTACGAGGTGCTGTCGATTTTGCACTTAATGGCAATGCTAGCATTATCACAAGCTAACTTGTTACTTCTTCCAAGAACATCTGCTGATGGTTATCAGCCAAAAGTAACAGAAGAGAGTAGACGAGCTTCCATTGATATTTTCCTGAAGGCAGCAGGATACCTTGATTGTGCAGTCCGACATGTTCTTCCACAGTTGCCTACTGAGCTCAAGACAAACCTGCCAGTTGACTTAGCAGAAGGAGTTCTTCGAGCGCTTTGCCTACAAGCATTAGGACAGGGTGTTGATATTCAACTTGGAATGGCAATTGACAGCACAAAAGCCACACTTGCAGTGAAGCGAAGGCTTGCATGTGAAATGGTAAAGTATTGGCAACAGGCTCAAGATAACCTTATGAACCTTCCCTTATTAAATGGTTGGGGTGAAAAGCATCGGCTCTTTGTGAAGTGGAAATATGTTGAGGCAAAGGCTGCTGCATATTACTATCATGGTTTGATCCTTGATGAGGGTAACACAGAAAAATTTCATGGGATGGCTGTAGCTGCCTTGCAAGCAGCAGATGAGTATTTTAAAGAAAGTAGGAAGGCATGCGACGCATTTAACGCTGCTCCCCCTTTGTCGAGAAATCCACCGCTCTGGGGAACCATGAAGTATCTATCTGAGAAAATTCCTAAAGATACTTCAAGCAAAGTGCGGATAAACCGTGATCTGTACTCACACGAAAAAATCATGGAGACAGCGCCAACATTGCCCGATTTTGCATTGGCCTTGAAACCGGATGAATTCCAACTTCCTCCAGTAGACCCCTCTTGGAACATGGAGCACATGAATAAGGGTTCCTACCAGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRO1

Protein Analysis

428

Amino Acids

47.96

Weight (kDa)

8.1

Isoelectric Point (pI)

46.67

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17940 AT1G17940 AT1G17940 AT1G17940 AT1G73390 AT1G73390 AT1G73390 AT1G73390 AT1G73390 AT1G73390 AT1G73390
fragaria_vesca	FvH4_5g18540 FvH4_5g18540 FvH4_5g18540
malus_domestica	MD06G1240300.v1.1 MD14G1246500.v1.1
prunus_persica	Prupe.5G244800_v2.0.a1 Prupe.5G244800_v2.0.a1 Prupe.5G244800_v2.0.a1
pyrus_communis	pycom06g21560 pycom14g20720
rosa_chinensis	RchiOBHm_Chr7g0176971
rosa_laevigata	RLG00000005567
rosa_multiflora	Rmu_sc0005310.1_g000037
rosa_roxburghii	Rroxscaffold_3G00276200
rosa_rugosa	Rorug06G0402600 Rorug06G0402700 Rorug06G0402700
rosa_samantha	Rh7BG002300 Rh7CG001700 Rh7DG002300
rosa_wichuraiana	Rw7G000150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	660
AccBSI	CCGCTC	1 cut(s)	1066
AccI	GTMKAC	2 cut(s)	553, 1233
AciI	CCGC	3 cut(s)	54, 1064, 1126
AclWI	GGATC	1 cut(s)	922
AcoI	YGGCCR	1 cut(s)	271
AcsI	RAATTY	3 cut(s)	953, 1098, 1214
AcuI	CTGAAG	1 cut(s)	599
AdeI	CACNNNGTG	1 cut(s)	131
AfaI	GTAC	2 cut(s)	437, 1145
AfeI	AGCGCT	1 cut(s)	687
AfiI	CCNNNNNNNGG	2 cut(s)	937, 1070
AflIII	ACRYGT	1 cut(s)	613
AgsI	TTSAA	5 cut(s)	265, 377, 725, 1116, 1204
AjnI	CCWGG	2 cut(s)	9, 85
AluBI	AGCT	8 cut(s)	239, 361, 419, 488, 560, 640, 974, 1279
AluI	AGCT	8 cut(s)	239, 361, 419, 488, 560, 640, 974, 1279
Alw21I	GWGCWC	2 cut(s)	642, 1257
Alw26I	GTCTC	3 cut(s)	19, 76, 1160
AlwI	GGATC	1 cut(s)	922
Ama87I	CYCGRG	1 cut(s)	311
Aor51HI	AGCGCT	1 cut(s)	687
AoxI	GGCC	3 cut(s)	50, 271, 1197
ApeKI	GCWGC	8 cut(s)	197, 239, 584, 902, 905, 974, 986, 1037
ApoI	RAATTY	3 cut(s)	953, 1098, 1214
AseI	ATTAAT	1 cut(s)	341
Asp700I	GAANNNNTTC	2 cut(s)	675, 954
AspLEI	GCGC	2 cut(s)	688, 1174
AspS9I	GGNCC	2 cut(s)	51, 209
AsuHPI	GGTGA	2 cut(s)	143, 866
AsuNHI	GCTAGC	1 cut(s)	472
AvaI	CYCGRG	1 cut(s)	311
AvaII	GGWCC	1 cut(s)	209
BanII	GRGCYC	1 cut(s)	642
BarI	GAAGNNNNNNTAC	4 cut(s)	226, 258, 544, 576
Bbv12I	GWGCWC	2 cut(s)	642, 1257
BbvI	GCAGC	8 cut(s)	184, 226, 596, 889, 892, 961, 998, 1024
BccI	CCATC	4 cut(s)	187, 344, 515, 958
BcgI	CGANNNNNNTGC	2 cut(s)	672, 706
BciT130I	CCWGG	2 cut(s)	11, 87
BciVI	GTATCC	1 cut(s)	584
BclI	TGATCA	1 cut(s)	133
BcoDI	GTCTC	3 cut(s)	19, 76, 1160
BfaI	CTAG	2 cut(s)	35, 473
BfmI	CTRYAG	1 cut(s)	969
BfoI	RGCGCY	2 cut(s)	689, 1175
BfuAI	ACCTGC	1 cut(s)	660
BfuI	GTATCC	1 cut(s)	584
BisI	GCNGC	8 cut(s)	198, 240, 585, 903, 906, 975, 987, 1038
BlsI	GCNGC	8 cut(s)	199, 241, 586, 904, 907, 976, 988, 1039
Bme1390I	CCNGG	2 cut(s)	11, 87
Bme18I	GGWCC	1 cut(s)	209
BmeT110I	CYCGRG	1 cut(s)	311
BmgT120I	GGNCC	2 cut(s)	51, 209
BmiI	GGNNCC	2 cut(s)	1075, 1270
BmrFI	CCNGG	2 cut(s)	11, 87
BmsI	GCATC	2 cut(s)	394, 871
BmtI	GCTAGC	1 cut(s)	476
BpmI	CTGGAG	1 cut(s)	1212
BpuEI	CTTGAG	2 cut(s)	626, 798
Bsa29I	ATCGAT	1 cut(s)	278
BsaBI	GATNNNNATC	1 cut(s)	525
BsaI	GGTCTC	1 cut(s)	19
BsaJI	CCNNGG	1 cut(s)	85
BsaWI	WCCGGW	1 cut(s)	1207
Bsc4I	CCNNNNNNNGG	2 cut(s)	937, 1070
Bse1I	ACTGG	2 cut(s)	656, 1229
Bse3DI	GCAATG	2 cut(s)	474, 1178
Bse8I	GATNNNNATC	1 cut(s)	525
BseBI	CCWGG	2 cut(s)	11, 87
BseCI	ATCGAT	1 cut(s)	278
BseDI	CCNNGG	1 cut(s)	85
BseGI	GGATG	4 cut(s)	11, 406, 969, 1216
BseJI	GATNNNNATC	1 cut(s)	525
BseLI	CCNNNNNNNGG	2 cut(s)	937, 1070
BseMI	GCAATG	2 cut(s)	474, 1178
BseMII	CTCAG	3 cut(s)	134, 627, 1083
BseNI	ACTGG	2 cut(s)	656, 1229
BseXI	GCAGC	8 cut(s)	184, 226, 596, 889, 892, 961, 998, 1024
BsgI	GTGCAG	1 cut(s)	624
Bsh1285I	CGRYCG	1 cut(s)	278
BshFI	GGCC	3 cut(s)	52, 273, 1199
BshVI	ATCGAT	1 cut(s)	278
BsiEI	CGRYCG	1 cut(s)	278
BsiHKAI	GWGCWC	2 cut(s)	642, 1257
BsiHKCI	CYCGRG	1 cut(s)	311
BsiSI	CCGG	1 cut(s)	1208
BslFI	GGGAC	1 cut(s)	68
BslI	CCNNNNNNNGG	2 cut(s)	937, 1070
BsmAI	GTCTC	3 cut(s)	19, 76, 1160
BsmFI	GGGAC	1 cut(s)	68
BsnI	GGCC	3 cut(s)	52, 273, 1199
Bso31I	GGTCTC	1 cut(s)	19
BsoBI	CYCGRG	1 cut(s)	311
Bsp1286I	GDGCHC	2 cut(s)	642, 1257
Bsp143I	GATC	5 cut(s)	133, 275, 279, 927, 1138
BspACI	CCGC	3 cut(s)	54, 1064, 1126
BspANI	GGCC	3 cut(s)	52, 273, 1199
BspCNI	CTCAG	3 cut(s)	133, 628, 1084
BspDI	ATCGAT	1 cut(s)	278
BspLI	GGNNCC	2 cut(s)	1075, 1270
BspMI	ACCTGC	1 cut(s)	660
BspOI	GCTAGC	1 cut(s)	476
BspPI	GGATC	1 cut(s)	922
BspQI	GCTCTTC	1 cut(s)	229
BspTNI	GGTCTC	1 cut(s)	19
BsrBI	CCGCTC	1 cut(s)	1066
BsrDI	GCAATG	2 cut(s)	474, 1178
BsrI	ACTGG	2 cut(s)	656, 1229
BssECI	CCNNGG	1 cut(s)	85
BssMI	GATC	5 cut(s)	133, 275, 279, 927, 1138
Bst2UI	CCWGG	2 cut(s)	11, 87
Bst4CI	ACNGT	2 cut(s)	627, 1136
Bst6I	CTCTTC	3 cut(s)	224, 229, 540
BstAPI	GCANNNNNTGC	1 cut(s)	902
BstC8I	GCNNGC	7 cut(s)	54, 306, 359, 474, 780, 984, 1021
BstDEI	CTNAG	4 cut(s)	120, 636, 664, 1092
BstENI	CCTNNNNNAGG	1 cut(s)	935
BstF5I	GGATG	4 cut(s)	11, 406, 969, 1216
BstH2I	RGCGCY	2 cut(s)	689, 1175
BstHHI	GCGC	2 cut(s)	688, 1174
BstKTI	GATC	5 cut(s)	136, 278, 282, 930, 1141
BstMAI	GTCTC	3 cut(s)	19, 76, 1160
BstMBI	GATC	5 cut(s)	133, 275, 279, 927, 1138
BstMCI	CGRYCG	1 cut(s)	278
BstMWI	GCNNNNNNNGC	9 cut(s)	473, 637, 761, 902, 974, 983, 1025, 1034, 1180
BstNI	CCWGG	2 cut(s)	11, 87
BstNSI	RCATGY	3 cut(s)	617, 786, 1023
BstSCI	CCNGG	2 cut(s)	9, 85
BstSFI	CTRYAG	1 cut(s)	969
BstV1I	GCAGC	8 cut(s)	184, 226, 596, 889, 892, 961, 998, 1024
BstXI	CCANNNNNNTGG	1 cut(s)	1068
Bsu15I	ATCGAT	1 cut(s)	278
BsuI	GTATCC	1 cut(s)	584
BsuRI	GGCC	3 cut(s)	52, 273, 1199
BsuTUI	ATCGAT	1 cut(s)	278
BtsCI	GGATG	4 cut(s)	11, 406, 969, 1216
BtsI	GCAGTG	1 cut(s)	771
BtsIMutI	CAGTG	1 cut(s)	771
BveI	ACCTGC	1 cut(s)	660
Cac8I	GCNNGC	7 cut(s)	54, 306, 359, 474, 780, 984, 1021
CfoI	GCGC	2 cut(s)	688, 1174
Cfr13I	GGNCC	2 cut(s)	51, 209
ClaI	ATCGAT	1 cut(s)	278
CseI	GACGC	1 cut(s)	1034
Csp6I	GTAC	2 cut(s)	436, 1144
CspCI	CAANNNNNGTGG	2 cut(s)	365, 400
CviQI	GTAC	2 cut(s)	436, 1144
DdeI	CTNAG	4 cut(s)	120, 636, 664, 1092
DpnI	GATC	5 cut(s)	135, 277, 281, 929, 1140
DpnII	GATC	5 cut(s)	133, 275, 279, 927, 1138
DraI	TTTAAA	1 cut(s)	1003
DraIII	CACNNNGTG	1 cut(s)	131
EaeI	YGGCCR	1 cut(s)	271
Eam1104I	CTCTTC	3 cut(s)	224, 229, 540
EarI	CTCTTC	3 cut(s)	224, 229, 540
Ecl136II	GAGCTC	1 cut(s)	640
Eco24I	GRGCYC	1 cut(s)	642
Eco31I	GGTCTC	1 cut(s)	19
Eco47I	GGWCC	1 cut(s)	209
Eco47III	AGCGCT	1 cut(s)	687
Eco53kI	GAGCTC	1 cut(s)	640
Eco57I	CTGAAG	1 cut(s)	599
Eco88I	CYCGRG	1 cut(s)	311
EcoICRI	GAGCTC	1 cut(s)	640
EcoNI	CCTNNNNNAGG	1 cut(s)	935
EcoRI	GAATTC	1 cut(s)	1214
EcoRII	CCWGG	2 cut(s)	9, 85
EcoT38I	GRGCYC	1 cut(s)	642
FalI	AAGNNNNNCTT	6 cut(s)	663, 695, 744, 776, 807, 839
FaqI	GGGAC	1 cut(s)	68
FauI	CCCGC	1 cut(s)	61
FbaI	TGATCA	1 cut(s)	133
FblI	GTMKAC	2 cut(s)	553, 1233
Fnu4HI	GCNGC	8 cut(s)	198, 240, 585, 903, 906, 975, 987, 1038
FokI	GGATG	4 cut(s)	18, 413, 976, 1223
FriOI	GRGCYC	1 cut(s)	642
Fsp4HI	GCNGC	8 cut(s)	198, 240, 585, 903, 906, 975, 987, 1038
FspBI	CTAG	2 cut(s)	35, 473
GlaI	GCGC	2 cut(s)	687, 1173
GluI	GCNGC	8 cut(s)	198, 240, 585, 903, 906, 975, 987, 1038
GsuI	CTGGAG	1 cut(s)	1212
HaeII	RGCGCY	2 cut(s)	689, 1175
HaeIII	GGCC	3 cut(s)	52, 273, 1199
HapII	CCGG	1 cut(s)	1208
HgaI	GACGC	1 cut(s)	1034
HhaI	GCGC	2 cut(s)	688, 1174
Hin6I	GCGC	2 cut(s)	686, 1172
HinP1I	GCGC	2 cut(s)	686, 1172
HincII	GTYRAC	1 cut(s)	661
HindII	GTYRAC	1 cut(s)	661
HinfI	GANTC	2 cut(s)	96, 178
HpaII	CCGG	1 cut(s)	1208
HphI	GGTGA	2 cut(s)	143, 866
Hpy166II	GTNNAC	3 cut(s)	554, 661, 1234
Hpy188I	TCNGA	3 cut(s)	213, 611, 1093
Hpy188III	TCNNGA	6 cut(s)	311, 395, 577, 643, 815, 1053
Hpy8I	GTNNAC	3 cut(s)	554, 661, 1234
Hpy99I	CGWCG	1 cut(s)	1028
HpyAV	CCTTC	7 cut(s)	42, 200, 574, 665, 768, 842, 1009
HpyCH4III	ACNGT	2 cut(s)	627, 1136
HpyCH4V	TGCA	9 cut(s)	170, 407, 457, 605, 764, 782, 908, 982, 1193
HpyF10VI	GCNNNNNNNGC	9 cut(s)	473, 637, 761, 902, 974, 983, 1025, 1034, 1180
HpyF3I	CTNAG	4 cut(s)	120, 636, 664, 1092
HspAI	GCGC	2 cut(s)	686, 1172
Ksp22I	TGATCA	1 cut(s)	133
Kzo9I	GATC	5 cut(s)	133, 275, 279, 927, 1138
LguI	GCTCTTC	1 cut(s)	229
LmnI	GCTCC	2 cut(s)	1045, 1252
Lsp1109I	GCAGC	8 cut(s)	184, 226, 596, 889, 892, 961, 998, 1024
LweI	GCATC	2 cut(s)	394, 871
MaeI	CTAG	2 cut(s)	35, 473
MaeIII	GTNAC	4 cut(s)	123, 495, 538, 941
MalI	GATC	5 cut(s)	135, 277, 281, 929, 1140
MbiI	CCGCTC	1 cut(s)	1066
MboI	GATC	5 cut(s)	133, 275, 279, 927, 1138
MboII	GAAGA	6 cut(s)	241, 246, 494, 557, 611, 671
MfeI	CAATTG	1 cut(s)	738
MhlI	GDGCHC	2 cut(s)	642, 1257
MluCI	AATT	6 cut(s)	216, 377, 738, 953, 1098, 1214
MlyI	GAGTC	1 cut(s)	172
MmeI	TCCRAC	3 cut(s)	236, 634, 1243
MnlI	CCTC	8 cut(s)	307, 391, 403, 433, 886, 931, 1236, 1249
MroXI	GAANNNNTTC	2 cut(s)	675, 954
MseI	TTAA	6 cut(s)	173, 341, 461, 842, 1002, 1032
MslI	CAYNNNNRTG	1 cut(s)	188
MspI	CCGG	1 cut(s)	1208
MspR9I	CCNGG	2 cut(s)	11, 87
MunI	CAATTG	1 cut(s)	738
MvaI	CCWGG	2 cut(s)	11, 87
MwoI	GCNNNNNNNGC	9 cut(s)	473, 637, 761, 902, 974, 983, 1025, 1034, 1180
NdeII	GATC	5 cut(s)	133, 275, 279, 927, 1138
NheI	GCTAGC	1 cut(s)	472
NlaIV	GGNNCC	2 cut(s)	1075, 1270
NmuCI	GTSAC	1 cut(s)	123
NspI	RCATGY	3 cut(s)	617, 786, 1023
PaeI	GCATGC	1 cut(s)	1023
PaeR7I	CTCGAG	1 cut(s)	311
PciI	ACATGT	1 cut(s)	613
PciSI	GCTCTTC	1 cut(s)	229
PcsI	WCGNNNNNNNCGW	1 cut(s)	435
PdmI	GAANNNNTTC	2 cut(s)	675, 954
PfeI	GAWTC	1 cut(s)	96
PkrI	GCNGC	8 cut(s)	199, 241, 586, 904, 907, 976, 988, 1039
Ple19I	CGATCG	1 cut(s)	278
PleI	GAGTC	1 cut(s)	172
PpsI	GAGTC	1 cut(s)	172
PscI	ACATGT	1 cut(s)	613
PshBI	ATTAAT	1 cut(s)	341
Psp124BI	GAGCTC	1 cut(s)	642
Psp6I	CCWGG	2 cut(s)	9, 85
PspGI	CCWGG	2 cut(s)	9, 85
PspN4I	GGNNCC	2 cut(s)	1075, 1270
PspPI	GGNCC	2 cut(s)	51, 209
PvuI	CGATCG	1 cut(s)	278
RsaI	GTAC	2 cut(s)	437, 1145
RsaNI	GTAC	2 cut(s)	436, 1144
RseI	CAYNNNNRTG	1 cut(s)	188
SacI	GAGCTC	1 cut(s)	642
SapI	GCTCTTC	1 cut(s)	229
SaqAI	TTAA	6 cut(s)	173, 341, 461, 842, 1002, 1032
SatI	GCNGC	8 cut(s)	198, 240, 585, 903, 906, 975, 987, 1038
Sau3AI	GATC	5 cut(s)	133, 275, 279, 927, 1138
Sau96I	GGNCC	2 cut(s)	51, 209
SchI	GAGTC	1 cut(s)	172
ScrFI	CCNGG	2 cut(s)	11, 87
SduI	GDGCHC	2 cut(s)	642, 1257
SfaNI	GCATC	2 cut(s)	394, 871
SfcI	CTRYAG	1 cut(s)	969
Sfr274I	CTCGAG	1 cut(s)	311
SinI	GGWCC	1 cut(s)	209
SlaI	CTCGAG	1 cut(s)	311
SmiMI	CAYNNNNRTG	1 cut(s)	188
SmlI	CTYRAG	3 cut(s)	311, 641, 813
SmoI	CTYRAG	3 cut(s)	311, 641, 813
SphI	GCATGC	1 cut(s)	1023
Sse9I	AATT	6 cut(s)	216, 377, 738, 953, 1098, 1214
SsiI	CCGC	3 cut(s)	54, 1064, 1126
SspMI	CTAG	2 cut(s)	35, 473
SstI	GAGCTC	1 cut(s)	642
StyD4I	CCNGG	2 cut(s)	9, 85
TaaI	ACNGT	2 cut(s)	627, 1136
TaqI	TCGA	5 cut(s)	278, 312, 449, 682, 1052
TasI	AATT	6 cut(s)	216, 377, 738, 953, 1098, 1214
TatI	WGTACW	1 cut(s)	1143
TfiI	GAWTC	1 cut(s)	96
Tru1I	TTAA	6 cut(s)	173, 341, 461, 842, 1002, 1032
Tru9I	TTAA	6 cut(s)	173, 341, 461, 842, 1002, 1032
TscAI	CASTG	1 cut(s)	771
TseFI	GTSAC	1 cut(s)	123
TseI	GCWGC	8 cut(s)	197, 239, 584, 902, 905, 974, 986, 1037
Tsp45I	GTSAC	1 cut(s)	123
TspDTI	ATGAA	6 cut(s)	359, 842, 947, 1094, 1227, 1274
TspRI	CASTG	1 cut(s)	771
VpaK11BI	GGWCC	1 cut(s)	209
VspI	ATTAAT	1 cut(s)	341
XagI	CCTNNNNNAGG	1 cut(s)	935
XapI	RAATTY	3 cut(s)	953, 1098, 1214
XceI	RCATGY	3 cut(s)	617, 786, 1023
XcmI	CCANNNNNNNNNTGG	1 cut(s)	151
XhoI	CTCGAG	1 cut(s)	311
XmiI	GTMKAC	2 cut(s)	553, 1233
XmnI	GAANNNNTTC	2 cut(s)	675, 954
XspI	CTAG	2 cut(s)	35, 473

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.