Rorug06G0402600

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004447
Physical Location & Seq
Forward (+)
54698644 .. 54700200
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0402600.1

Sequence Viewer

Length: 849 bp
ATGGAACCAACTAGGAATGTTGTTTCTATACCAGAGGCTCCTCCGAGTCCAAACACCCCACTGCAGGATCCAAACGAAAGCAAGAAGAAACTAGTGATACTCGAAGATCAAGACGAAGAGGAAATTGAAGAGGTGAATCGTCCCAATACTGTCCTGTTATTAGATCTAGAGCTTTCTAATACTAGTAAGGATTCAACTTCAAACAGTAGTGGGTTCAACCCAGAACTCAATCTCATCGACAGTCTGCAAATGGGTGGTTCATCATCAGAAACAGATCAAAGCAGCCCTGGTGATCAAGGGGCAGCAGAGCCAAGAGTTTTCTCATGCAACTATTGTCAAAGAAAGTTCTACAGCTCACAAGCACTTGGAGGACACCAAAATGCACACAAAAGAGAGAGGACTCTAGCAAAGAGAGGACAAAGGCAGTTGATGGCGTCAACAGCAGCGGCCGCTGGTTTTGGTGGTTACCCTTACTTCCATCATCACAACCACCCTTACTTTTCGAGCATGGCTTCTCTGCCTCTTCATGGTGCCTATAACAACAAGTCTTTGGATATTCAGGTCCACTCAATGATTCACAAGCCTAGTGGTAGTGGTAGTGGTATTGGGTTTGGAAGTTCGTATGGAAATCGTGGGCACAAGTCATCAAGACCCATTTTTGATCAACAACCAGCGATTGGAAAACTGGCACCACAGAATTTTCAAACCACATCATCATCAAGAGGTAGTAGTACTAGTAGTGTGGGAAGATTTGACATGGCAAATGTTGGTTGGTTGGAAAACGGCAGTCGTTTCAGGAGTGGTCAACACGAAGAAATGAAGAACCTAGACTTGTCCCTCAAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

282

Amino Acids

30.75

Weight (kDa)

6.6

Isoelectric Point (pI)

58.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 530, 688
AccB7I CCANNNNNTGG 1 cut(s) 677
AciI CCGC 2 cut(s) 446, 450
AclWI GGATC 2 cut(s) 62, 75
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 1 cut(s) 697
AcyI GRCGYC 1 cut(s) 434
AfaI GTAC 1 cut(s) 733
AfiI CCNNNNNNNGG 3 cut(s) 64, 527, 677
AgsI TTSAA 5 cut(s) 128, 195, 201, 217, 704
AhlI ACTAGT 3 cut(s) 91, 182, 734
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 3 cut(s) 172, 354, 844
AluI AGCT 3 cut(s) 172, 354, 844
AlwI GGATC 2 cut(s) 62, 75
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 3 cut(s) 282, 302, 443
ApoI RAATTY 1 cut(s) 697
AspS9I GGNCC 1 cut(s) 562
AsuHPI GGTGA 2 cut(s) 145, 302
AvaII GGWCC 1 cut(s) 562
BaeGI GKGCMC 1 cut(s) 639
BamHI GGATCC 1 cut(s) 67
BanI GGYRCC 2 cut(s) 530, 688
BbvI GCAGC 3 cut(s) 294, 314, 455
BccI CCATC 2 cut(s) 424, 486
BceAI ACGGC 1 cut(s) 799
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 2 cut(s) 292, 661
BcuI ACTAGT 3 cut(s) 91, 182, 734
BfaI CTAG 9 cut(s) 12, 92, 167, 183, 404, 585, 735, 827, 847
BfmI CTRYAG 2 cut(s) 62, 349
BglII AGATCT 1 cut(s) 163
BisI GCNGC 5 cut(s) 283, 303, 444, 447, 450
BlsI GCNGC 5 cut(s) 284, 304, 445, 448, 451
BmcAI AGTACT 1 cut(s) 733
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 562
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 5 cut(s) 6, 39, 69, 532, 690
BmrFI CCNGG 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 824
BsaHI GRCGYC 1 cut(s) 434
BsaJI CCNNGG 1 cut(s) 286
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 527, 677
Bse1I ACTGG 1 cut(s) 690
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 286
BseLI CCNNNNNNNGG 3 cut(s) 64, 527, 677
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 639
BseX3I CGGCCG 1 cut(s) 447
BseXI GCAGC 3 cut(s) 294, 314, 455
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 1 cut(s) 449
BshNI GGYRCC 2 cut(s) 530, 688
BsiEI CGRYCG 1 cut(s) 450
BslFI GGGAC 2 cut(s) 126, 820
BslI CCNNNNNNNGG 3 cut(s) 64, 527, 677
BsmFI GGGAC 2 cut(s) 126, 820
BsnI GGCC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 6 cut(s) 67, 106, 163, 274, 292, 661
BspACI CCGC 2 cut(s) 446, 450
BspANI GGCC 1 cut(s) 449
BspLI GGNNCC 5 cut(s) 6, 39, 69, 532, 690
BspMAI CTGCAG 1 cut(s) 66
BspPI GGATC 2 cut(s) 62, 75
BspT107I GGYRCC 2 cut(s) 530, 688
BsrI ACTGG 1 cut(s) 690
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 6 cut(s) 67, 106, 163, 274, 292, 661
BssNI GRCGYC 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 3 cut(s) 151, 206, 242
Bst6I CTCTTC 3 cut(s) 111, 123, 528
BstACI GRCGYC 1 cut(s) 434
BstEII GGTNACC 1 cut(s) 464
BstKTI GATC 6 cut(s) 70, 109, 166, 277, 295, 664
BstMBI GATC 6 cut(s) 67, 106, 163, 274, 292, 661
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 2 cut(s) 440, 449
BstNI CCWGG 1 cut(s) 288
BstPI GGTNACC 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 2 cut(s) 62, 349
BstSLI GKGCMC 1 cut(s) 639
BstV1I GCAGC 3 cut(s) 294, 314, 455
BstX2I RGATCY 2 cut(s) 67, 163
BstYI RGATCY 2 cut(s) 67, 163
BstZI CGGCCG 1 cut(s) 447
BsuRI GGCC 1 cut(s) 449
BtsI GCAGTG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 59
CciNI GCGGCCGC 1 cut(s) 447
Cfr13I GGNCC 1 cut(s) 562
CseI GACGC 1 cut(s) 423
Csp6I GTAC 1 cut(s) 732
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 4 cut(s) 324, 508, 527, 757
CviJI RGCY 9 cut(s) 38, 172, 285, 310, 354, 449, 512, 583, 844
CviKI_1 RGCY 9 cut(s) 38, 172, 285, 310, 354, 449, 512, 583, 844
CviQI GTAC 1 cut(s) 732
DpnI GATC 6 cut(s) 69, 108, 165, 276, 294, 663
DpnII GATC 6 cut(s) 67, 106, 163, 274, 292, 661
EaeI YGGCCR 1 cut(s) 447
EagI CGGCCG 1 cut(s) 447
Eam1104I CTCTTC 3 cut(s) 111, 123, 528
EarI CTCTTC 3 cut(s) 111, 123, 528
EclXI CGGCCG 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 562
Eco52I CGGCCG 1 cut(s) 447
Eco91I GGTNACC 1 cut(s) 464
EcoO65I GGTNACC 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 286
FaeI CATG 4 cut(s) 327, 511, 530, 760
FaiI YATR 7 cut(s) 29, 325, 509, 528, 537, 624, 758
FaqI GGGAC 2 cut(s) 126, 820
FatI CATG 4 cut(s) 323, 507, 526, 756
FbaI TGATCA 2 cut(s) 292, 661
Fnu4HI GCNGC 5 cut(s) 283, 303, 444, 447, 450
Fsp4HI GCNGC 5 cut(s) 283, 303, 444, 447, 450
FspBI CTAG 9 cut(s) 12, 92, 167, 183, 404, 585, 735, 827, 847
GluI GCNGC 5 cut(s) 283, 303, 444, 447, 450
HaeIII GGCC 1 cut(s) 449
HgaI GACGC 1 cut(s) 423
Hin1I GRCGYC 1 cut(s) 434
Hin1II CATG 4 cut(s) 327, 511, 530, 760
HincII GTYRAC 2 cut(s) 438, 806
HindII GTYRAC 2 cut(s) 438, 806
HinfI GANTC 5 cut(s) 46, 136, 191, 400, 574
HphI GGTGA 2 cut(s) 145, 302
Hpy166II GTNNAC 3 cut(s) 438, 565, 806
Hpy188I TCNGA 2 cut(s) 45, 268
Hpy188III TCNNGA 5 cut(s) 110, 167, 648, 720, 796
Hpy8I GTNNAC 3 cut(s) 438, 565, 806
HpyCH4III ACNGT 3 cut(s) 151, 206, 242
HpyCH4V TGCA 4 cut(s) 64, 247, 327, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 449
Hsp92I GRCGYC 1 cut(s) 434
Hsp92II CATG 4 cut(s) 327, 511, 530, 760
Ksp22I TGATCA 2 cut(s) 292, 661
Kzo9I GATC 6 cut(s) 67, 106, 163, 274, 292, 661
LmnI GCTCC 1 cut(s) 43
Lsp1109I GCAGC 3 cut(s) 294, 314, 455
MaeI CTAG 9 cut(s) 12, 92, 167, 183, 404, 585, 735, 827, 847
MaeIII GTNAC 1 cut(s) 464
MalI GATC 6 cut(s) 69, 108, 165, 276, 294, 663
MboI GATC 6 cut(s) 67, 106, 163, 274, 292, 661
MboII GAAGA 8 cut(s) 97, 116, 128, 140, 515, 759, 824, 832
MflI RGATCY 2 cut(s) 67, 163
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 2 cut(s) 123, 697
MlyI GAGTC 2 cut(s) 55, 394
MmeI TCCRAC 1 cut(s) 756
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 2 cut(s) 446, 452
MspR9I CCNGG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 440, 449
NdeII GATC 6 cut(s) 67, 106, 163, 274, 292, 661
NlaIII CATG 4 cut(s) 327, 511, 530, 760
NlaIV GGNNCC 5 cut(s) 6, 39, 69, 532, 690
NotI GCGGCCGC 1 cut(s) 447
PfeI GAWTC 3 cut(s) 136, 191, 574
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 5 cut(s) 284, 304, 445, 448, 451
PleI GAGTC 2 cut(s) 54, 394
PpsI GAGTC 2 cut(s) 54, 394
Psp6I CCWGG 1 cut(s) 286
PspEI GGTNACC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 5 cut(s) 6, 39, 69, 532, 690
PspPI GGNCC 1 cut(s) 562
PstI CTGCAG 1 cut(s) 66
PsuI RGATCY 2 cut(s) 67, 163
RsaI GTAC 1 cut(s) 733
RsaNI GTAC 1 cut(s) 732
RseI CAYNNNNRTG 1 cut(s) 378
SatI GCNGC 5 cut(s) 283, 303, 444, 447, 450
Sau3AI GATC 6 cut(s) 67, 106, 163, 274, 292, 661
Sau96I GGNCC 1 cut(s) 562
ScaI AGTACT 1 cut(s) 733
SchI GAGTC 2 cut(s) 55, 394
ScrFI CCNGG 1 cut(s) 288
SduI GDGCHC 1 cut(s) 639
SetI ASST 7 cut(s) 135, 174, 356, 564, 727, 828, 846
SfcI CTRYAG 2 cut(s) 62, 349
SinI GGWCC 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
SpeI ACTAGT 3 cut(s) 91, 182, 734
Sse9I AATT 2 cut(s) 123, 697
SsiI CCGC 2 cut(s) 446, 450
SspMI CTAG 9 cut(s) 12, 92, 167, 183, 404, 585, 735, 827, 847
StyD4I CCNGG 1 cut(s) 286
TaaI ACNGT 3 cut(s) 151, 206, 242
TaqI TCGA 3 cut(s) 102, 237, 503
TasI AATT 2 cut(s) 123, 697
TatI WGTACW 1 cut(s) 731
TauI GCSGC 2 cut(s) 449, 452
TfiI GAWTC 3 cut(s) 136, 191, 574
TscAI CASTG 1 cut(s) 66
TseI GCWGC 3 cut(s) 282, 302, 443
TspDTI ATGAA 3 cut(s) 249, 515, 833
TspRI CASTG 1 cut(s) 66
Van91I CCANNNNNTGG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 562
XapI RAATTY 1 cut(s) 697
XbaI TCTAGA 1 cut(s) 166
XspI CTAG 9 cut(s) 12, 92, 167, 183, 404, 585, 735, 827, 847
ZrmI AGTACT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.