RLG00000005567

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004447
Physical Location & Seq
Forward (+)
68585245 .. 68591132
5888 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005567

Sequence Viewer

Length: 1365 bp
ATGCCAATTCAGTACACACATTTCAACGTAACTTTTCGCCACTCAGTCGACCTTCTGATTAGAGACGAGATACAACAATACAAGGACATGGGATGCCTGGTGTCAACACCGAAAGATACAGTTGGAAATAGGAGGAGGCCATCAAACATCGGTGAAGTTTCTGTTTATGTTCCTGGTCTGCGAATACCTAAACCCGTTGATTTCTCTCTGTCACTTGGTGATCATTTGTCCAAGAGTTTAGTAGAACGTCTATCTGCTTTAAGAACTCGTATAGTTGTAATGGCTGGCCAAGAAGGTCCCACAATCACAAGAACAAGGAGAAGAACCCAACACGGTGGTTCAACATTAGCTGATCTCCAGCAGGCCCTCGAGGATTACTTACCTGTTCTTTTGGGATTAATGAAAGATGGAAGCCAGCTACAACACAAGGTTCAATTTGTGTGGGTAAATCAAGAGGATGATGCAGAGGAAACAGCTATGTCTAATGGATGGTACGAGGTGATGTCAATTTTGCATTTAATGGCAATGCTATCACTATCACAAGCTAACTTATTACTTCTTCCGAGAACATCTGCTGACGGTCACCAGCCAAAAGTATCAGAAGAGAGTAGACGAGCTTCTGTTGATATATTCCTGAAGGCTGCAGGACACCTTGACTGTGCTGTCCGACATGTTCTCCCGCAGTTGCCTGCTGAGCTCAAGAGAAACCTTCCAGTTGATCTAGCAGAGGGAGTTCTTCGAGCTCTTTGCTTACAAGCACTAGGACAGGGTGTTGATATTCAACTTGGAATGGCAATTGATAGTACAAAAGCCACTCTTGCAGTGAAGCGAAGGCTTGCATGTGAGATGGTAAAGTATTGGCAGCAGGCTCAAGATAACATTATGAACCTTCCATTATTAAATGGTTGGGGTGAAAAGCATAAACTCTTTGTGAAGTGGAAATATGTTGAGGCAAAGGCTGCAGCATATTATTATCATGGTTTAATCCTCGATGAGGGTAACACAGAAAAATTTCATGGAATGGCTGTAGCTGCTTTGCAAGCAGCAGACGAGTATTTTAAAGAAAGTAAGAAGGCATGTGAGGCATTTAATGCTGCTTCTCCTTTGTCCAGAAACCCACCGCTTTGGGGAACCATGAAATATCTATCTGAGAAAATCCCAAAAGATACTTCAAGCAAAGTGCGAATAAACCGTGATCTGTACTCTCATGCAAAAATCATGGAGACGGCACCGACATTGCCTGACTTTGCTCTGGCCTTGAAACCCGACGAATATCAACTTCCTCCAGTGGATCCCTCTTGGAACATGGAGCACACAAATAGGGGACCTACAGCTTCCAACCAGCTCAGGAATGACAGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

455

Amino Acids

50.98

Weight (kDa)

8.6

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 126 - 359 6.4e-13 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
AbsI CCTCGAGG 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 1228
AccI GTMKAC 2 cut(s) 48, 610
AciI CCGC 2 cut(s) 680, 1121
AclWI GGATC 2 cut(s) 1286, 1299
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 1010
AcuI CTGAAG 1 cut(s) 656
AdeI CACNNNGTG 1 cut(s) 218
AfaI GTAC 4 cut(s) 14, 494, 805, 1202
AfiI CCNNNNNNNGG 2 cut(s) 994, 1127
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 6 cut(s) 25, 342, 434, 782, 1173, 1261
AjnI CCWGG 2 cut(s) 96, 172
AjuI GAANNNNNNNTTGG 2 cut(s) 1153, 1185
Alw21I GWGCWC 3 cut(s) 699, 745, 1314
Alw26I GTCTC 2 cut(s) 57, 1217
AlwI GGATC 2 cut(s) 1286, 1299
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 4 cut(s) 137, 286, 363, 1254
ApeKI GCWGC 7 cut(s) 641, 862, 959, 962, 1031, 1043, 1094
ApoI RAATTY 1 cut(s) 1010
AseI ATTAAT 1 cut(s) 398
Asp700I GAANNNNTTC 1 cut(s) 1011
AspS9I GGNCC 3 cut(s) 296, 364, 1325
AsuHPI GGTGA 5 cut(s) 164, 230, 511, 575, 923
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 296, 1325
BalI TGGCCA 1 cut(s) 288
BamHI GGATCC 1 cut(s) 1291
BanI GGYRCC 1 cut(s) 1228
BanII GRGCYC 2 cut(s) 699, 745
BarI GAAGNNNNNNTAC 2 cut(s) 601, 633
Bbv12I GWGCWC 3 cut(s) 699, 745, 1314
BbvI GCAGC 7 cut(s) 628, 874, 946, 974, 1018, 1055, 1081
BccI CCATC 4 cut(s) 148, 401, 483, 841
BceAI ACGGC 1 cut(s) 1242
BcgI CGANNNNNNTGC 2 cut(s) 729, 763
BciT130I CCWGG 2 cut(s) 98, 174
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 2 cut(s) 57, 1217
BfaI CTAG 2 cut(s) 722, 761
BfmI CTRYAG 4 cut(s) 642, 960, 1026, 1329
BisI GCNGC 7 cut(s) 642, 863, 960, 963, 1032, 1044, 1095
BlpI GCTNAGC 1 cut(s) 693
BlsI GCNGC 7 cut(s) 643, 864, 961, 964, 1033, 1045, 1096
Bme1390I CCNGG 2 cut(s) 98, 174
Bme18I GGWCC 2 cut(s) 296, 1325
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 296, 364, 1325
BmiI GGNNCC 5 cut(s) 298, 1132, 1230, 1293, 1326
BmrFI CCNGG 2 cut(s) 98, 174
BmsI GCATC 2 cut(s) 83, 451
BpmI CTGGAG 2 cut(s) 341, 1269
Bpu10I CCTNAGC 1 cut(s) 1346
Bpu1102I GCTNAGC 1 cut(s) 693
BpuEI CTTGAG 2 cut(s) 683, 855
BsaXI ACNNNNNCTCC 2 cut(s) 660, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 994, 1127
Bse1I ACTGG 2 cut(s) 713, 1286
Bse3DI GCAATG 2 cut(s) 531, 1235
BseBI CCWGG 2 cut(s) 98, 174
BseGI GGATG 3 cut(s) 98, 463, 494
BseLI CCNNNNNNNGG 2 cut(s) 994, 1127
BseMI GCAATG 2 cut(s) 531, 1235
BseMII CTCAG 4 cut(s) 57, 684, 1140, 1360
BseNI ACTGG 2 cut(s) 713, 1286
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 7 cut(s) 628, 874, 946, 974, 1018, 1055, 1081
BshFI GGCC 4 cut(s) 139, 288, 365, 1256
BshNI GGYRCC 1 cut(s) 1228
BsiHKAI GWGCWC 3 cut(s) 699, 745, 1314
BsiHKCI CYCGRG 1 cut(s) 368
BslFI GGGAC 2 cut(s) 282, 1338
BslI CCNNNNNNNGG 2 cut(s) 994, 1127
BsmAI GTCTC 2 cut(s) 57, 1217
BsmBI CGTCTC 2 cut(s) 57, 1217
BsmFI GGGAC 2 cut(s) 282, 1338
BsnI GGCC 4 cut(s) 139, 288, 365, 1256
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 3 cut(s) 699, 745, 1314
Bsp143I GATC 5 cut(s) 220, 352, 718, 1195, 1291
Bsp1720I GCTNAGC 1 cut(s) 693
BspACI CCGC 2 cut(s) 680, 1121
BspANI GGCC 4 cut(s) 139, 288, 365, 1256
BspCNI CTCAG 4 cut(s) 56, 685, 1141, 1359
BspLI GGNNCC 5 cut(s) 298, 1132, 1230, 1293, 1326
BspMAI CTGCAG 2 cut(s) 646, 964
BspPI GGATC 2 cut(s) 1286, 1299
BspT107I GGYRCC 1 cut(s) 1228
BsrDI GCAATG 2 cut(s) 531, 1235
BsrI ACTGG 2 cut(s) 713, 1286
BssMI GATC 5 cut(s) 220, 352, 718, 1195, 1291
Bst2UI CCWGG 2 cut(s) 98, 174
Bst4CI ACNGT 5 cut(s) 121, 335, 581, 659, 1193
Bst6I CTCTTC 1 cut(s) 597
BstAPI GCANNNNNTGC 2 cut(s) 959, 1091
BstC8I GCNNGC 7 cut(s) 286, 363, 416, 690, 837, 867, 1041
BstDEI CTNAG 4 cut(s) 43, 693, 1149, 1346
BstEII GGTNACC 1 cut(s) 581
BstENI CCTNNNNNAGG 1 cut(s) 992
BstF5I GGATG 3 cut(s) 98, 463, 494
BstKTI GATC 5 cut(s) 223, 355, 721, 1198, 1294
BstMAI GTCTC 2 cut(s) 57, 1217
BstMBI GATC 5 cut(s) 220, 352, 718, 1195, 1291
BstMWI GCNNNNNNNGC 7 cut(s) 694, 818, 959, 1031, 1040, 1082, 1091
BstNI CCWGG 2 cut(s) 98, 174
BstNSI RCATGY 3 cut(s) 674, 843, 1080
BstPI GGTNACC 1 cut(s) 581
BstSCI CCNGG 2 cut(s) 96, 172
BstSFI CTRYAG 4 cut(s) 642, 960, 1026, 1329
BstV1I GCAGC 7 cut(s) 628, 874, 946, 974, 1018, 1055, 1081
BstX2I RGATCY 1 cut(s) 1291
BstXI CCANNNNNNTGG 2 cut(s) 335, 1125
BstYI RGATCY 1 cut(s) 1291
BsuRI GGCC 4 cut(s) 139, 288, 365, 1256
BtsCI GGATG 3 cut(s) 98, 463, 494
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 828, 1293
Cac8I GCNNGC 7 cut(s) 286, 363, 416, 690, 837, 867, 1041
Cfr13I GGNCC 3 cut(s) 296, 364, 1325
Csp6I GTAC 4 cut(s) 13, 493, 804, 1201
CspCI CAANNNNNGTGG 2 cut(s) 422, 457
CviQI GTAC 4 cut(s) 13, 493, 804, 1201
DdeI CTNAG 4 cut(s) 43, 693, 1149, 1346
DpnI GATC 5 cut(s) 222, 354, 720, 1197, 1293
DpnII GATC 5 cut(s) 220, 352, 718, 1195, 1291
DraI TTTAAA 1 cut(s) 1060
DraIII CACNNNGTG 1 cut(s) 218
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Ecl136II GAGCTC 2 cut(s) 697, 743
Eco24I GRGCYC 2 cut(s) 699, 745
Eco47I GGWCC 2 cut(s) 296, 1325
Eco53kI GAGCTC 2 cut(s) 697, 743
Eco57I CTGAAG 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 368
Eco91I GGTNACC 1 cut(s) 581
EcoICRI GAGCTC 2 cut(s) 697, 743
EcoNI CCTNNNNNAGG 1 cut(s) 992
EcoO109I RGGNCCY 3 cut(s) 296, 364, 1325
EcoO65I GGTNACC 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 96, 172
EcoT38I GRGCYC 2 cut(s) 699, 745
Esp3I CGTCTC 2 cut(s) 57, 1217
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 2 cut(s) 282, 1338
FauI CCCGC 1 cut(s) 687
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 2 cut(s) 48, 610
Fnu4HI GCNGC 7 cut(s) 642, 863, 960, 963, 1032, 1044, 1095
FokI GGATG 3 cut(s) 105, 470, 501
FriOI GRGCYC 2 cut(s) 699, 745
Fsp4HI GCNGC 7 cut(s) 642, 863, 960, 963, 1032, 1044, 1095
FspBI CTAG 2 cut(s) 722, 761
GluI GCNGC 7 cut(s) 642, 863, 960, 963, 1032, 1044, 1095
GsuI CTGGAG 2 cut(s) 341, 1269
HaeIII GGCC 4 cut(s) 139, 288, 365, 1256
HincII GTYRAC 2 cut(s) 49, 105
HindII GTYRAC 2 cut(s) 49, 105
HphI GGTGA 5 cut(s) 164, 230, 511, 575, 923
Hpy166II GTNNAC 4 cut(s) 15, 49, 105, 611
Hpy188I TCNGA 5 cut(s) 57, 564, 601, 668, 1150
Hpy188III TCNNGA 6 cut(s) 452, 634, 700, 872, 1110, 1348
Hpy8I GTNNAC 4 cut(s) 15, 49, 105, 611
Hpy99I CGWCG 1 cut(s) 1271
HpyAV CCTTC 7 cut(s) 62, 287, 631, 719, 825, 899, 1066
HpyCH4III ACNGT 5 cut(s) 121, 335, 581, 659, 1193
HpyCH4IV ACGT 2 cut(s) 27, 247
HpyCH4V TGCA 8 cut(s) 464, 514, 644, 821, 839, 962, 1039, 1211
HpyF10VI GCNNNNNNNGC 7 cut(s) 694, 818, 959, 1031, 1040, 1082, 1091
HpyF3I CTNAG 4 cut(s) 43, 693, 1149, 1346
HpySE526I ACGT 2 cut(s) 27, 247
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 5 cut(s) 220, 352, 718, 1195, 1291
LmnI GCTCC 1 cut(s) 1309
Lsp1109I GCAGC 7 cut(s) 628, 874, 946, 974, 1018, 1055, 1081
LweI GCATC 2 cut(s) 83, 451
MaeI CTAG 2 cut(s) 722, 761
MaeII ACGT 2 cut(s) 27, 247
MaeIII GTNAC 4 cut(s) 28, 210, 581, 998
MalI GATC 5 cut(s) 222, 354, 720, 1197, 1293
MboI GATC 5 cut(s) 220, 352, 718, 1195, 1291
MboII GAAGA 4 cut(s) 333, 551, 614, 728
MfeI CAATTG 1 cut(s) 795
MflI RGATCY 1 cut(s) 1291
MhlI GDGCHC 3 cut(s) 699, 745, 1314
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 5 cut(s) 6, 434, 507, 795, 1010
MluNI TGGCCA 1 cut(s) 288
MmeI TCCRAC 3 cut(s) 103, 691, 1362
Mox20I TGGCCA 1 cut(s) 288
MroXI GAANNNNTTC 1 cut(s) 1011
MscI TGGCCA 1 cut(s) 288
MseI TTAA 7 cut(s) 260, 398, 518, 899, 983, 1059, 1089
Msp20I TGGCCA 1 cut(s) 288
MspR9I CCNGG 2 cut(s) 98, 174
MunI CAATTG 1 cut(s) 795
MvaI CCWGG 2 cut(s) 98, 174
MwoI GCNNNNNNNGC 7 cut(s) 694, 818, 959, 1031, 1040, 1082, 1091
NdeII GATC 5 cut(s) 220, 352, 718, 1195, 1291
NlaIV GGNNCC 5 cut(s) 298, 1132, 1230, 1293, 1326
NmuCI GTSAC 2 cut(s) 210, 581
NspI RCATGY 3 cut(s) 674, 843, 1080
PaeR7I CTCGAG 1 cut(s) 368
PciI ACATGT 1 cut(s) 670
PdmI GAANNNNTTC 1 cut(s) 1011
PkrI GCNGC 7 cut(s) 643, 864, 961, 964, 1033, 1045, 1096
PpuMI RGGWCCY 2 cut(s) 296, 1325
PscI ACATGT 1 cut(s) 670
PshBI ATTAAT 1 cut(s) 398
Psp124BI GAGCTC 2 cut(s) 699, 745
Psp5II RGGWCCY 2 cut(s) 296, 1325
Psp6I CCWGG 2 cut(s) 96, 172
PspEI GGTNACC 1 cut(s) 581
PspGI CCWGG 2 cut(s) 96, 172
PspN4I GGNNCC 5 cut(s) 298, 1132, 1230, 1293, 1326
PspPI GGNCC 3 cut(s) 296, 364, 1325
PspPPI RGGWCCY 2 cut(s) 296, 1325
PspXI VCTCGAGB 1 cut(s) 368
PstI CTGCAG 2 cut(s) 646, 964
PsuI RGATCY 1 cut(s) 1291
RsaI GTAC 4 cut(s) 14, 494, 805, 1202
RsaNI GTAC 4 cut(s) 13, 493, 804, 1201
SacI GAGCTC 2 cut(s) 699, 745
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 7 cut(s) 260, 398, 518, 899, 983, 1059, 1089
SatI GCNGC 7 cut(s) 642, 863, 960, 963, 1032, 1044, 1095
Sau3AI GATC 5 cut(s) 220, 352, 718, 1195, 1291
Sau96I GGNCC 3 cut(s) 296, 364, 1325
ScrFI CCNGG 2 cut(s) 98, 174
SduI GDGCHC 3 cut(s) 699, 745, 1314
SfaNI GCATC 2 cut(s) 83, 451
SfcI CTRYAG 4 cut(s) 642, 960, 1026, 1329
Sfr274I CTCGAG 1 cut(s) 368
SinI GGWCC 2 cut(s) 296, 1325
SlaI CTCGAG 1 cut(s) 368
SmlI CTYRAG 3 cut(s) 368, 698, 870
SmoI CTYRAG 3 cut(s) 368, 698, 870
Sse9I AATT 5 cut(s) 6, 434, 507, 795, 1010
SsiI CCGC 2 cut(s) 680, 1121
SspMI CTAG 2 cut(s) 722, 761
SstI GAGCTC 2 cut(s) 699, 745
StyD4I CCNGG 2 cut(s) 96, 172
TaaI ACNGT 5 cut(s) 121, 335, 581, 659, 1193
TaiI ACGT 2 cut(s) 30, 250
TaqI TCGA 4 cut(s) 48, 369, 739, 990
TasI AATT 5 cut(s) 6, 434, 507, 795, 1010
TatI WGTACW 3 cut(s) 12, 803, 1200
Tru1I TTAA 7 cut(s) 260, 398, 518, 899, 983, 1059, 1089
Tru9I TTAA 7 cut(s) 260, 398, 518, 899, 983, 1059, 1089
TscAI CASTG 2 cut(s) 828, 1293
TseFI GTSAC 2 cut(s) 210, 581
TseI GCWGC 7 cut(s) 641, 862, 959, 962, 1031, 1043, 1094
Tsp45I GTSAC 2 cut(s) 210, 581
TspDTI ATGAA 4 cut(s) 416, 899, 1004, 1151
TspRI CASTG 2 cut(s) 828, 1293
VpaK11BI GGWCC 2 cut(s) 296, 1325
VspI ATTAAT 1 cut(s) 398
XagI CCTNNNNNAGG 1 cut(s) 992
XapI RAATTY 1 cut(s) 1010
XceI RCATGY 3 cut(s) 674, 843, 1080
XhoI CTCGAG 1 cut(s) 368
XmiI GTMKAC 2 cut(s) 48, 610
XmnI GAANNNNTTC 1 cut(s) 1011
XspI CTAG 2 cut(s) 722, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.