Prupe.5G244800_v2.0.a1

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0004447
Physical Location & Seq
Forward (+)
18338269 .. 18345811
7543 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G244800.3

Sequence Viewer

Length: 1284 bp
ATGGGATGCCTGGTGTCAACACCGAAAGATACAGTAGGAAATAGGAGGAGGCCGGCAAATATTGGGGAAGTTTCTGTCTATGTTCCTGGTTTGCGAATCCCTAAACCTGTTGATTTCTCTCAGTCACTCGGTGATCACTTATCCAAGAGTTTAGTAGAACGCATATCTGCTTTAAGAACTCGTATAGTTGTAATGGCTGGCCAAGAAGGTCCTACAATTACAAGAACAAGGAGAAAAACTGCTACCCAACATGGTGGTTCAACATTAGCTGATCTTCAGCAGGCTCTTGAGGACTACTTGCCTGTCCTTTTGGGATTAATGAAAGATGGAAGCCAGCTACAACACAAGGTACAATTTGTGTGGGTAAATCAAGAGGATGATGCAGAGGAAACAGCTATGTCTAATGCATGGTACGAGGTGCTGTCCATCTTGCACTTAATGGCAATGCTATCGTTCTTACAAGCTAATTTGTTACTTCTTCCAAGAACATCTGCTGATGGTTATCAGCCAAAAGTAACAGAAGAGAGTAGACGAGCTTCAATTGATATTTTCATAAAGGCAGCAGGATATCTTGATTGTGCTGTCCGACATGTTCACCCACAGTTGCCTACTGAGCTCAAGAGAAACCTGCCAGTCGACCTAGCAGAAGGAGTTCTTCGGGCACTTTGCCTACAAGCATTAGGACAGGGTGTTGATATTCAACTTGGGATGGCAATTGATAGCACAAAAGCTACTCTTGCAGTGAAGCGAAGGCTTGCATGTGAAATGGTGAAGTATTGGCAGCAGGCTCAAGATAACATTATGAACCTTCCATTATTAAATGGTTGGGGTGAAAAGCATCGGCTCTTTGTGAAGTGGAAATATGTTGAGGCAAAGGCTGCTGCATATTATTATCATGGTTTGATCCTTGACGAGGGTAACACCGAAAAATTTCATGGGATGGCTGTAGCTGCTTTGCAAGCAGCAGATGAGTATTTTAAAGAAAGTAAGAAAGCATGTGACGCATTTCATGCTGCTCCTCCTTTGTCGAGAAATCCACCGCTCTGGGGAACCATGAAATATCTGTCTGAAAAAATTCCAAAAGATACTTCGAGCAAAGTGCGGATAAACCGTGACCTCTACTCACACGAAAAAATCATGGAGACAGCACCAACATTGCCTGACTTTGCTTTGGCCCTGAAACCGGATGAATACCAACTTCCTCCGGTGGACCCGTCCTGGAACATGGAGCACATGAATAAGGGACAGACTGGTTCCAACCAGCTCAGGAATGACAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

428

Amino Acids

47.87

Weight (kDa)

8.61

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 636
AccBSI CCGCTC 1 cut(s) 1042
AccI GTMKAC 2 cut(s) 529, 636
AciI CCGC 2 cut(s) 1040, 1102
AclWI GGATC 1 cut(s) 898
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 2 cut(s) 929, 1074
AcuI CTGAAG 1 cut(s) 260
AdeI CACNNNGTG 1 cut(s) 131
AfaI GTAC 2 cut(s) 351, 413
AfiI CCNNNNNNNGG 3 cut(s) 913, 1046, 1183
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 3 cut(s) 261, 540, 701
AjnI CCWGG 3 cut(s) 9, 85, 1217
AjuI GAANNNNNNNTTGG 2 cut(s) 1072, 1104
AluBI AGCT 9 cut(s) 269, 337, 395, 464, 536, 616, 731, 950, 1264
AluI AGCT 9 cut(s) 269, 337, 395, 464, 536, 616, 731, 950, 1264
Alw21I GWGCWC 2 cut(s) 618, 1233
Alw26I GTCTC 1 cut(s) 1136
AlwI GGATC 1 cut(s) 898
AoxI GGCC 3 cut(s) 50, 199, 1173
ApeKI GCWGC 7 cut(s) 560, 781, 878, 881, 950, 962, 1013
ApoI RAATTY 2 cut(s) 929, 1074
AseI ATTAAT 1 cut(s) 317
Asp700I GAANNNNTTC 3 cut(s) 651, 930, 1074
AspS9I GGNCC 3 cut(s) 209, 1174, 1210
AsuHPI GGTGA 4 cut(s) 143, 587, 781, 842
AvaII GGWCC 2 cut(s) 209, 1210
BaeGI GKGCMC 1 cut(s) 664
BalI TGGCCA 1 cut(s) 201
BanII GRGCYC 1 cut(s) 618
BarI GAAGNNNNNNTAC 2 cut(s) 520, 552
Bbv12I GWGCWC 2 cut(s) 618, 1233
BbvI GCAGC 7 cut(s) 572, 793, 865, 868, 937, 974, 1000
BccI CCATC 5 cut(s) 320, 434, 491, 703, 934
BcgI CGANNNNNNTGC 4 cut(s) 432, 466, 1081, 1115
BciT130I CCWGG 3 cut(s) 11, 87, 1219
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 1136
BfaI CTAG 1 cut(s) 641
BfmI CTRYAG 1 cut(s) 945
BfuAI ACCTGC 1 cut(s) 636
BisI GCNGC 7 cut(s) 561, 782, 879, 882, 951, 963, 1014
BlsI GCNGC 7 cut(s) 562, 783, 880, 883, 952, 964, 1015
Bme1390I CCNGG 3 cut(s) 11, 87, 1219
Bme18I GGWCC 2 cut(s) 209, 1210
BmgT120I GGNCC 3 cut(s) 209, 1174, 1210
BmiI GGNNCC 3 cut(s) 1051, 1212, 1255
BmrFI CCNGG 3 cut(s) 11, 87, 1219
BmsI GCATC 2 cut(s) 370, 847
Bpu10I CCTNAGC 1 cut(s) 1265
BpuEI CTTGAG 3 cut(s) 308, 602, 774
BsaBI GATNNNNATC 1 cut(s) 501
BsaWI WCCGGW 2 cut(s) 1183, 1204
Bsc4I CCNNNNNNNGG 3 cut(s) 913, 1046, 1183
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 2 cut(s) 632, 1255
Bse3DI GCAATG 2 cut(s) 450, 1154
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 3 cut(s) 11, 87, 1219
BseGI GGATG 5 cut(s) 11, 382, 714, 945, 1192
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 3 cut(s) 913, 1046, 1183
BseMI GCAATG 2 cut(s) 450, 1154
BseMII CTCAG 3 cut(s) 134, 603, 1279
BseNI ACTGG 2 cut(s) 632, 1255
BseRI GAGGAG 2 cut(s) 61, 1008
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 7 cut(s) 572, 793, 865, 868, 937, 974, 1000
BshFI GGCC 3 cut(s) 52, 201, 1175
BsiHKAI GWGCWC 2 cut(s) 618, 1233
BsiSI CCGG 3 cut(s) 53, 1184, 1205
BslFI GGGAC 1 cut(s) 1257
BslI CCNNNNNNNGG 3 cut(s) 913, 1046, 1183
BsmAI GTCTC 1 cut(s) 1136
BsmFI GGGAC 1 cut(s) 1257
BsnI GGCC 3 cut(s) 52, 201, 1175
Bsp1286I GDGCHC 3 cut(s) 618, 664, 1233
Bsp143I GATC 3 cut(s) 133, 271, 903
BspACI CCGC 2 cut(s) 1040, 1102
BspANI GGCC 3 cut(s) 52, 201, 1175
BspCNI CTCAG 3 cut(s) 133, 604, 1278
BspLI GGNNCC 3 cut(s) 1051, 1212, 1255
BspMI ACCTGC 1 cut(s) 636
BspPI GGATC 1 cut(s) 898
BsrBI CCGCTC 1 cut(s) 1042
BsrDI GCAATG 2 cut(s) 450, 1154
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 2 cut(s) 632, 1255
BssAI RCCGGY 1 cut(s) 52
BssMI GATC 3 cut(s) 133, 271, 903
Bst2UI CCWGG 3 cut(s) 11, 87, 1219
Bst4CI ACNGT 3 cut(s) 34, 603, 1112
Bst6I CTCTTC 1 cut(s) 516
BstAPI GCANNNNNTGC 2 cut(s) 878, 1010
BstC8I GCNNGC 7 cut(s) 54, 199, 282, 335, 756, 786, 960
BstDEI CTNAG 3 cut(s) 120, 612, 1265
BstENI CCTNNNNNAGG 1 cut(s) 911
BstF5I GGATG 5 cut(s) 11, 382, 714, 945, 1192
BstKTI GATC 3 cut(s) 136, 274, 906
BstMAI GTCTC 1 cut(s) 1136
BstMBI GATC 3 cut(s) 133, 271, 903
BstMWI GCNNNNNNNGC 7 cut(s) 613, 737, 878, 950, 959, 1001, 1010
BstNI CCWGG 3 cut(s) 11, 87, 1219
BstNSI RCATGY 3 cut(s) 593, 762, 999
BstSCI CCNGG 3 cut(s) 9, 85, 1217
BstSFI CTRYAG 1 cut(s) 945
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 7 cut(s) 572, 793, 865, 868, 937, 974, 1000
BstXI CCANNNNNNTGG 2 cut(s) 254, 1044
BsuRI GGCC 3 cut(s) 52, 201, 1175
BtsCI GGATG 5 cut(s) 11, 382, 714, 945, 1192
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 1 cut(s) 747
BveI ACCTGC 1 cut(s) 636
Cac8I GCNNGC 7 cut(s) 54, 199, 282, 335, 756, 786, 960
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 3 cut(s) 209, 1174, 1210
CseI GACGC 1 cut(s) 1010
Csp6I GTAC 2 cut(s) 350, 412
CspCI CAANNNNNGTGG 2 cut(s) 341, 376
CviQI GTAC 2 cut(s) 350, 412
DdeI CTNAG 3 cut(s) 120, 612, 1265
DpnI GATC 3 cut(s) 135, 273, 905
DpnII GATC 3 cut(s) 133, 271, 903
DraI TTTAAA 1 cut(s) 979
DraIII CACNNNGTG 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Ecl136II GAGCTC 1 cut(s) 616
Eco24I GRGCYC 1 cut(s) 618
Eco32I GATATC 1 cut(s) 569
Eco47I GGWCC 2 cut(s) 209, 1210
Eco53kI GAGCTC 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 616
EcoNI CCTNNNNNAGG 1 cut(s) 911
EcoO109I RGGNCCY 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 9, 85, 1217
EcoRV GATATC 1 cut(s) 569
EcoT22I ATGCAT 1 cut(s) 409
EcoT38I GRGCYC 1 cut(s) 618
FalI AAGNNNNNCTT 4 cut(s) 639, 671, 720, 752
FaqI GGGAC 1 cut(s) 1257
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 2 cut(s) 529, 636
Fnu4HI GCNGC 7 cut(s) 561, 782, 879, 882, 951, 963, 1014
FokI GGATG 5 cut(s) 18, 389, 721, 952, 1199
FriOI GRGCYC 1 cut(s) 618
Fsp4HI GCNGC 7 cut(s) 561, 782, 879, 882, 951, 963, 1014
FspBI CTAG 1 cut(s) 641
GluI GCNGC 7 cut(s) 561, 782, 879, 882, 951, 963, 1014
HaeIII GGCC 3 cut(s) 52, 201, 1175
HapII CCGG 3 cut(s) 53, 1184, 1205
HgaI GACGC 1 cut(s) 1010
HincII GTYRAC 2 cut(s) 18, 637
HindII GTYRAC 2 cut(s) 18, 637
HinfI GANTC 1 cut(s) 96
HpaII CCGG 3 cut(s) 53, 1184, 1205
HphI GGTGA 4 cut(s) 143, 587, 781, 842
Hpy166II GTNNAC 5 cut(s) 18, 530, 595, 637, 1210
Hpy188I TCNGA 2 cut(s) 587, 1069
Hpy188III TCNNGA 7 cut(s) 287, 371, 572, 619, 791, 1029, 1267
Hpy8I GTNNAC 5 cut(s) 18, 530, 595, 637, 1210
HpyAV CCTTC 4 cut(s) 200, 641, 744, 818
HpyCH4III ACNGT 3 cut(s) 34, 603, 1112
HpyCH4V TGCA 7 cut(s) 383, 407, 433, 740, 758, 884, 958
HpyF10VI GCNNNNNNNGC 7 cut(s) 613, 737, 878, 950, 959, 1001, 1010
HpyF3I CTNAG 3 cut(s) 120, 612, 1265
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 133, 271, 903
LmnI GCTCC 2 cut(s) 1021, 1228
Lsp1109I GCAGC 7 cut(s) 572, 793, 865, 868, 937, 974, 1000
LweI GCATC 2 cut(s) 370, 847
MaeI CTAG 1 cut(s) 641
MaeIII GTNAC 6 cut(s) 123, 471, 514, 917, 998, 1112
MalI GATC 3 cut(s) 135, 273, 905
MbiI CCGCTC 1 cut(s) 1042
MboI GATC 3 cut(s) 133, 271, 903
MboII GAAGA 4 cut(s) 266, 470, 533, 647
MfeI CAATTG 2 cut(s) 540, 714
MhlI GDGCHC 3 cut(s) 618, 664, 1233
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 7 cut(s) 216, 353, 466, 540, 714, 929, 1074
MluNI TGGCCA 1 cut(s) 201
MmeI TCCRAC 2 cut(s) 610, 1281
Mox20I TGGCCA 1 cut(s) 201
Mph1103I ATGCAT 1 cut(s) 409
MroNI GCCGGC 1 cut(s) 52
MroXI GAANNNNTTC 3 cut(s) 651, 930, 1074
MscI TGGCCA 1 cut(s) 201
MseI TTAA 5 cut(s) 173, 317, 437, 818, 978
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 3 cut(s) 53, 1184, 1205
MspR9I CCNGG 3 cut(s) 11, 87, 1219
MunI CAATTG 2 cut(s) 540, 714
MvaI CCWGG 3 cut(s) 11, 87, 1219
MwoI GCNNNNNNNGC 7 cut(s) 613, 737, 878, 950, 959, 1001, 1010
NaeI GCCGGC 1 cut(s) 54
NdeII GATC 3 cut(s) 133, 271, 903
NgoMIV GCCGGC 1 cut(s) 52
NlaIV GGNNCC 3 cut(s) 1051, 1212, 1255
NmuCI GTSAC 3 cut(s) 123, 998, 1112
NsiI ATGCAT 1 cut(s) 409
NspI RCATGY 3 cut(s) 593, 762, 999
PciI ACATGT 1 cut(s) 589
PdiI GCCGGC 1 cut(s) 54
PdmI GAANNNNTTC 3 cut(s) 651, 930, 1074
PfeI GAWTC 1 cut(s) 96
PfoI TCCNGGA 1 cut(s) 1217
PkrI GCNGC 7 cut(s) 562, 783, 880, 883, 952, 964, 1015
PpuMI RGGWCCY 1 cut(s) 209
PscI ACATGT 1 cut(s) 589
PshBI ATTAAT 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 209
Psp6I CCWGG 3 cut(s) 9, 85, 1217
PspGI CCWGG 3 cut(s) 9, 85, 1217
PspN4I GGNNCC 3 cut(s) 1051, 1212, 1255
PspPI GGNCC 3 cut(s) 209, 1174, 1210
PspPPI RGGWCCY 1 cut(s) 209
RsaI GTAC 2 cut(s) 351, 413
RsaNI GTAC 2 cut(s) 350, 412
SacI GAGCTC 1 cut(s) 618
SalI GTCGAC 1 cut(s) 635
SaqAI TTAA 5 cut(s) 173, 317, 437, 818, 978
SatI GCNGC 7 cut(s) 561, 782, 879, 882, 951, 963, 1014
Sau3AI GATC 3 cut(s) 133, 271, 903
Sau96I GGNCC 3 cut(s) 209, 1174, 1210
ScrFI CCNGG 3 cut(s) 11, 87, 1219
SduI GDGCHC 3 cut(s) 618, 664, 1233
SfaNI GCATC 2 cut(s) 370, 847
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 2 cut(s) 209, 1210
SmlI CTYRAG 3 cut(s) 287, 617, 789
SmoI CTYRAG 3 cut(s) 287, 617, 789
Sse9I AATT 7 cut(s) 216, 353, 466, 540, 714, 929, 1074
SsiI CCGC 2 cut(s) 1040, 1102
SspI AATATT 1 cut(s) 61
SspMI CTAG 1 cut(s) 641
SstI GAGCTC 1 cut(s) 618
StyD4I CCNGG 3 cut(s) 9, 85, 1217
TaaI ACNGT 3 cut(s) 34, 603, 1112
TaqI TCGA 3 cut(s) 636, 1028, 1091
TasI AATT 7 cut(s) 216, 353, 466, 540, 714, 929, 1074
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 5 cut(s) 173, 317, 437, 818, 978
Tru9I TTAA 5 cut(s) 173, 317, 437, 818, 978
TscAI CASTG 1 cut(s) 747
TseFI GTSAC 3 cut(s) 123, 998, 1112
TseI GCWGC 7 cut(s) 560, 781, 878, 881, 950, 962, 1013
Tsp45I GTSAC 3 cut(s) 123, 998, 1112
TspDTI ATGAA 8 cut(s) 335, 541, 818, 923, 998, 1070, 1203, 1250
TspRI CASTG 1 cut(s) 747
VpaK11BI GGWCC 2 cut(s) 209, 1210
VspI ATTAAT 1 cut(s) 317
XagI CCTNNNNNAGG 1 cut(s) 911
XapI RAATTY 2 cut(s) 929, 1074
XceI RCATGY 3 cut(s) 593, 762, 999
XmiI GTMKAC 2 cut(s) 529, 636
XmnI GAANNNNTTC 3 cut(s) 651, 930, 1074
XspI CTAG 1 cut(s) 641
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.