FvH4_5g19610

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010777
Physical Location & Seq
Reverse (-)
11473398 .. 11475131
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19610.t1

Sequence Viewer

Length: 1734 bp
ATGGCAGAGCTAGAATCAGAGTCATACACCAAACCCCAAAAGGTGGTGTCATCAGATCAAAACCATGAAAACCAAAGTAAGTTCTACAATCATTTTCTGTACAAGATTGCTCTAGTTGTAGTTTTCTTCGTTATTCTCCCACTTTTCCCATCACAAGCTCCTGAGTTCATAAACCAAAGTCTTATCACCAGAAGCTGGGAGCTTCTTCATCTCCTCCTAGTTGGTATAGCTGTGTCTTATGGCTTATTTAGCAGACTAAACGAAAAAGTAGAGAAAGAAAACAACACCAAATTCGACAATGCTCACTCCTATGTGTCTAGAATTCTTCAGGTGCCCTCAGTTTTTGATGATGAGGCAGAAACCTCACCTGGACTTGATGAAAGCAAGGTCCAGGCATGGAGTAGTCAGTATTTTAGAAATGAACCTGTGGTGGTTGTGGCTCAAGAACACTCTGTTCTTGATGAACATAGGGATAGCAGTTCAATACATGGTGAAAAGCCATTGCTTTTGCCTGTTAGGAGCCTAAAACAACGTGTTCCTGACCAGGAGACGATAGAATCTGTTGATGAATCTAGTGTTACTTCTGGTGGGGCTCTGAGTAGGTCTAATTCTAGGTCTGGTTCAAGAAGGTTTTCGAGCAGATCAATTAAGGCTAGAGCTGGTGAAACTGGGGGTCTAGATCATCAAGAATTGGAGGAGAAGTTGAAGGAGAGTGTTGTGCTTCCTTCTCCAATACCGTGGCGATCAAGATCAGGAAGGTTGGAAGTGAGGGAGGATCTTGTTAGTAGTACTCCAATGGAGGAAGCTGAGTTTAACCGCCTAGATCCTCGGGGAGTTTCGAGGTCCCAAAGCTCTCATTCATCTCGATCCGATTCAGTGTCTTCATCACCAAAGCTGTCTCCTTCAACATCACTTTCGTCTCCAAAGAAGTTATCTCCTGCACCCTCATTGTCATCAGAAGCTCAAGCCAAGAGTGTTGAGGATGGGGGGAGGAAGAGGAGCTTTTACAAGAGTTCTATTCCCCCACCTCCTCCACCCCCACCGATGTTTTATAAATCATCATCATTGAGGCCAAGCAGTGATGAGGTTTCTTATGAGAAAGATTTGAGAAGAAGCTTCACTAGTGAAGCAAAGAACTTGAATAGGAGCAATGGAGAATTTATGATGGGCAGAGTTAATTCAGGGCTTGAAACAAAGCATAGGCTGAGCCATGTTGATGGCATTTCAATGGCAAAGTCTGTCAGAACAACCAGAGCTGGAGAACCAGGATATGTAAACGGGAAGGTGGAGCAAAGCGCTAAGGAAGTTGAAGCAAATGTGGTGGAGGATGTAACAAGAAAGAGGGTGGGGTTCAATGAATCTTCATTCTGGACTGAGAAGCTGAGCCATGAAAGCAGCATCCCCAACAACCCCAAGTCATCGGCTTTTGAAGAATTCTCTGAGGAAGATGAGAAGGAAGATCTTTTCGACAAGGTGGTTATGGAATCAGATGAAGAAACCGAAAGCGAGGGTGATGACACTGAAGGAGATTTTGCTCCTAAAGACATTGGAGGGAGCCCAAAGCCAAGTCCAAGGCCCTATCAACCTGCTTCTGGCAATGCAAGTGACGGAGGGCCTGATGTGGATAAGAAGGCAGATGAGTTCATAGCTAAATTCAGGGAGCAGATTCGGCTTCAGAGAATAGATTCGATCAAGAAATCGAGTGCTCAGATTAGTAAAAACTTGTCCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

578

Amino Acids

64.17

Weight (kDa)

5.67

Isoelectric Point (pI)

65.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 539 - 569 1.6e-13 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1053
Acc36I ACCTGC 1 cut(s) 1594
AccB1I GGYRCC 1 cut(s) 331
AccB7I CCANNNNNTGG 2 cut(s) 43, 195
AciI CCGC 1 cut(s) 817
AclWI GGATC 3 cut(s) 783, 818, 861
AcsI RAATTY 5 cut(s) 290, 321, 1157, 1433, 1652
AcuI CTGAAG 3 cut(s) 311, 1542, 1658
AfaI GTAC 2 cut(s) 101, 790
AfeI AGCGCT 1 cut(s) 1297
AfiI CCNNNNNNNGG 3 cut(s) 43, 195, 1592
AflIII ACRYGT 1 cut(s) 532
AhlI ACTAGT 1 cut(s) 1121
AjnI CCWGG 5 cut(s) 367, 390, 543, 1264, 1727
Alw21I GWGCWC 1 cut(s) 1708
Alw26I GTCTC 3 cut(s) 542, 903, 924
AlwI GGATC 3 cut(s) 783, 818, 861
AlwNI CAGNNNCTG 1 cut(s) 195
Ama87I CYCGRG 1 cut(s) 828
Aor51HI AGCGCT 1 cut(s) 1297
AoxI GGCC 3 cut(s) 1070, 1574, 1613
ApeKI GCWGC 1 cut(s) 1395
ApoI RAATTY 5 cut(s) 290, 321, 1157, 1433, 1652
Asp700I GAANNNNTTC 2 cut(s) 631, 1684
AspLEI GCGC 1 cut(s) 1298
AspS9I GGNCC 4 cut(s) 388, 843, 1575, 1613
AsuHPI GGTGA 6 cut(s) 178, 357, 503, 674, 879, 1523
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 2 cut(s) 388, 843
BaeGI GKGCMC 1 cut(s) 336
BanI GGYRCC 1 cut(s) 331
BanII GRGCYC 2 cut(s) 595, 1559
BbsI GAAGAC 1 cut(s) 873
Bbv12I GWGCWC 1 cut(s) 1708
BbvI GCAGC 1 cut(s) 1407
BccI CCATC 4 cut(s) 157, 977, 1159, 1211
BciT130I CCWGG 5 cut(s) 369, 392, 545, 1266, 1729
BcoDI GTCTC 3 cut(s) 542, 903, 924
BcuI ACTAGT 1 cut(s) 1121
BfoI RGCGCY 1 cut(s) 1299
BfuAI ACCTGC 1 cut(s) 1594
BglII AGATCT 1 cut(s) 1459
BisI GCNGC 1 cut(s) 1396
BlpI GCTNAGC 2 cut(s) 1205, 1382
BlsI GCNGC 1 cut(s) 1397
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 5 cut(s) 369, 392, 545, 1266, 1729
Bme18I GGWCC 2 cut(s) 388, 843
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 4 cut(s) 388, 843, 1575, 1613
BmiI GGNNCC 4 cut(s) 333, 521, 845, 1556
BmrFI CCNGG 5 cut(s) 369, 392, 545, 1266, 1729
BmrI ACTGGG 1 cut(s) 678
BmsI GCATC 1 cut(s) 1407
BmuI ACTGGG 1 cut(s) 678
BpiI GAAGAC 1 cut(s) 873
BpmI CTGGAG 1 cut(s) 1278
Bpu10I CCTNAGC 1 cut(s) 1299
Bpu1102I GCTNAGC 2 cut(s) 1205, 1382
BpuEI CTTGAG 2 cut(s) 426, 948
BsaBI GATNNNNATC 1 cut(s) 748
BsaJI CCNNGG 3 cut(s) 737, 827, 1571
BsaXI ACNNNNNCTCC 2 cut(s) 686, 716
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 195, 1592
Bse1I ACTGG 1 cut(s) 673
Bse3DI GCAATG 3 cut(s) 500, 1156, 1603
Bse8I GATNNNNATC 1 cut(s) 748
BseBI CCWGG 5 cut(s) 369, 392, 545, 1266, 1729
BseDI CCNNGG 3 cut(s) 737, 827, 1571
BseGI GGATG 3 cut(s) 988, 1333, 1398
BseJI GATNNNNATC 1 cut(s) 748
BseLI CCNNNNNNNGG 3 cut(s) 43, 195, 1592
BseMI GCAATG 3 cut(s) 500, 1156, 1603
BseMII CTCAG 9 cut(s) 153, 351, 587, 798, 1196, 1365, 1373, 1431, 1721
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 4 cut(s) 203, 710, 1012, 1020
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 1407
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 924
BshFI GGCC 3 cut(s) 1072, 1576, 1615
BshNI GGYRCC 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 1708
BsiHKCI CYCGRG 1 cut(s) 828
BslFI GGGAC 1 cut(s) 829
BslI CCNNNNNNNGG 3 cut(s) 43, 195, 1592
BsmAI GTCTC 3 cut(s) 542, 903, 924
BsmBI CGTCTC 2 cut(s) 542, 924
BsmFI GGGAC 1 cut(s) 829
BsnI GGCC 3 cut(s) 1072, 1576, 1615
BsoBI CYCGRG 1 cut(s) 828
Bsp1286I GDGCHC 4 cut(s) 336, 595, 1559, 1708
Bsp1407I TGTACA 1 cut(s) 99
Bsp1720I GCTNAGC 2 cut(s) 1205, 1382
BspACI CCGC 1 cut(s) 817
BspANI GGCC 3 cut(s) 1072, 1576, 1615
BspCNI CTCAG 9 cut(s) 154, 350, 588, 799, 1197, 1366, 1374, 1432, 1720
BspLI GGNNCC 4 cut(s) 333, 521, 845, 1556
BspMI ACCTGC 1 cut(s) 1594
BspPI GGATC 3 cut(s) 783, 818, 861
BspT107I GGYRCC 1 cut(s) 331
BsrDI GCAATG 3 cut(s) 500, 1156, 1603
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 1 cut(s) 673
BssECI CCNNGG 3 cut(s) 737, 827, 1571
BssT1I CCWWGG 1 cut(s) 1571
Bst2UI CCWGG 5 cut(s) 369, 392, 545, 1266, 1729
Bst4CI ACNGT 1 cut(s) 738
Bst6I CTCTTC 1 cut(s) 989
BstAUI TGTACA 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 737
BstF5I GGATG 3 cut(s) 988, 1333, 1398
BstH2I RGCGCY 1 cut(s) 1299
BstHHI GCGC 1 cut(s) 1298
BstMAI GTCTC 3 cut(s) 542, 903, 924
BstMWI GCNNNNNNNGC 3 cut(s) 249, 1392, 1669
BstNI CCWGG 5 cut(s) 369, 392, 545, 1266, 1729
BstSCI CCNGG 5 cut(s) 367, 390, 543, 1264, 1727
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 1407
BstV2I GAAGAC 1 cut(s) 873
BstX2I RGATCY 3 cut(s) 775, 823, 1459
BstXI CCANNNNNNTGG 2 cut(s) 738, 1217
BstYI RGATCY 3 cut(s) 775, 823, 1459
BsuRI GGCC 3 cut(s) 1072, 1576, 1615
BtgI CCRYGG 1 cut(s) 737
BtsCI GGATG 3 cut(s) 988, 1333, 1398
BtsI GCAGTG 1 cut(s) 1084
BtsIMutI CAGTG 3 cut(s) 882, 1084, 1518
BveI ACCTGC 1 cut(s) 1594
CaiI CAGNNNCTG 1 cut(s) 195
CfoI GCGC 1 cut(s) 1298
Cfr13I GGNCC 4 cut(s) 388, 843, 1575, 1613
Csp6I GTAC 2 cut(s) 100, 789
CspCI CAANNNNNGTGG 2 cut(s) 1302, 1337
CviAII CATG 5 cut(s) 65, 396, 488, 1211, 1388
CviQI GTAC 2 cut(s) 100, 789
Eam1104I CTCTTC 1 cut(s) 989
EarI CTCTTC 1 cut(s) 989
Eco130I CCWWGG 1 cut(s) 1571
Eco24I GRGCYC 2 cut(s) 595, 1559
Eco47I GGWCC 2 cut(s) 388, 843
Eco47III AGCGCT 1 cut(s) 1297
Eco57I CTGAAG 3 cut(s) 311, 1542, 1658
Eco88I CYCGRG 1 cut(s) 828
EcoO109I RGGNCCY 3 cut(s) 843, 1575, 1613
EcoRI GAATTC 2 cut(s) 321, 1433
EcoRII CCWGG 5 cut(s) 367, 390, 543, 1264, 1727
EcoT14I CCWWGG 1 cut(s) 1571
EcoT38I GRGCYC 2 cut(s) 595, 1559
ErhI CCWWGG 1 cut(s) 1571
Esp3I CGTCTC 2 cut(s) 542, 924
FaeI CATG 5 cut(s) 68, 399, 491, 1214, 1391
FalI AAGNNNNNCTT 2 cut(s) 986, 1018
FaqI GGGAC 1 cut(s) 829
FatI CATG 5 cut(s) 64, 395, 487, 1210, 1387
Fnu4HI GCNGC 1 cut(s) 1396
FokI GGATG 3 cut(s) 995, 1340, 1385
FriOI GRGCYC 2 cut(s) 595, 1559
Fsp4HI GCNGC 1 cut(s) 1396
GlaI GCGC 1 cut(s) 1297
GluI GCNGC 1 cut(s) 1396
GsaI CCCAGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 1278
HaeII RGCGCY 1 cut(s) 1299
HaeIII GGCC 3 cut(s) 1072, 1576, 1615
HhaI GCGC 1 cut(s) 1298
Hin1II CATG 5 cut(s) 68, 399, 491, 1214, 1391
Hin6I GCGC 1 cut(s) 1296
HinP1I GCGC 1 cut(s) 1296
HindIII AAGCTT 1 cut(s) 1114
HinfI GANTC 9 cut(s) 14, 20, 557, 569, 872, 1358, 1484, 1666, 1685
HphI GGTGA 6 cut(s) 178, 357, 503, 674, 879, 1523
Hpy166II GTNNAC 1 cut(s) 1276
Hpy8I GTNNAC 1 cut(s) 1276
HpyAV CCTTC 9 cut(s) 621, 700, 735, 750, 912, 1276, 1447, 1517, 1624
HpyCH4III ACNGT 1 cut(s) 738
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 2 cut(s) 941, 1601
HpyF10VI GCNNNNNNNGC 3 cut(s) 249, 1392, 1669
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 5 cut(s) 68, 399, 491, 1214, 1391
HspAI GCGC 1 cut(s) 1296
LmnI GCTCC 9 cut(s) 163, 199, 519, 999, 1146, 1288, 1540, 1554, 1660
Lsp1109I GCAGC 1 cut(s) 1407
LweI GCATC 1 cut(s) 1407
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 3 cut(s) 577, 1330, 1604
MflI RGATCY 3 cut(s) 775, 823, 1459
MhlI GDGCHC 4 cut(s) 336, 595, 1559, 1708
MluCI AATT 9 cut(s) 290, 321, 607, 645, 689, 1157, 1177, 1433, 1652
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 1 cut(s) 741
MroXI GAANNNNTTC 2 cut(s) 631, 1684
MseI TTAA 3 cut(s) 648, 813, 1176
MslI CAYNNNNRTG 3 cut(s) 309, 1215, 1226
MspR9I CCNGG 5 cut(s) 369, 392, 545, 1266, 1729
MvaI CCWGG 5 cut(s) 369, 392, 545, 1266, 1729
MwoI GCNNNNNNNGC 3 cut(s) 249, 1392, 1669
NlaIII CATG 5 cut(s) 68, 399, 491, 1214, 1391
NlaIV GGNNCC 4 cut(s) 333, 521, 845, 1556
NmuCI GTSAC 1 cut(s) 1604
PdmI GAANNNNTTC 2 cut(s) 631, 1684
PfeI GAWTC 8 cut(s) 14, 557, 569, 872, 1358, 1484, 1666, 1685
PflMI CCANNNNNTGG 2 cut(s) 43, 195
PkrI GCNGC 1 cut(s) 1397
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 843
PsiI TTATAA 1 cut(s) 1053
Psp5II RGGWCCY 1 cut(s) 843
Psp6I CCWGG 5 cut(s) 367, 390, 543, 1264, 1727
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 5 cut(s) 367, 390, 543, 1264, 1727
PspN4I GGNNCC 4 cut(s) 333, 521, 845, 1556
PspPI GGNCC 4 cut(s) 388, 843, 1575, 1613
PspPPI RGGWCCY 1 cut(s) 843
PstNI CAGNNNCTG 1 cut(s) 195
PsuI RGATCY 3 cut(s) 775, 823, 1459
RsaI GTAC 2 cut(s) 101, 790
RsaNI GTAC 2 cut(s) 100, 789
RseI CAYNNNNRTG 3 cut(s) 309, 1215, 1226
SaqAI TTAA 3 cut(s) 648, 813, 1176
SatI GCNGC 1 cut(s) 1396
Sau96I GGNCC 4 cut(s) 388, 843, 1575, 1613
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 5 cut(s) 369, 392, 545, 1266, 1729
SduI GDGCHC 4 cut(s) 336, 595, 1559, 1708
SfaNI GCATC 1 cut(s) 1407
SinI GGWCC 2 cut(s) 388, 843
SmiMI CAYNNNNRTG 3 cut(s) 309, 1215, 1226
SmlI CTYRAG 2 cut(s) 441, 963
SmoI CTYRAG 2 cut(s) 441, 963
SpeI ACTAGT 1 cut(s) 1121
Sse9I AATT 9 cut(s) 290, 321, 607, 645, 689, 1157, 1177, 1433, 1652
SsiI CCGC 1 cut(s) 817
StyD4I CCNGG 5 cut(s) 367, 390, 543, 1264, 1727
StyI CCWWGG 1 cut(s) 1571
TaaI ACNGT 1 cut(s) 738
TaiI ACGT 1 cut(s) 535
TaqI TCGA 7 cut(s) 294, 635, 839, 865, 1467, 1688, 1700
TasI AATT 9 cut(s) 290, 321, 607, 645, 689, 1157, 1177, 1433, 1652
TatI WGTACW 2 cut(s) 99, 788
TfiI GAWTC 8 cut(s) 14, 557, 569, 872, 1358, 1484, 1666, 1685
Tru1I TTAA 3 cut(s) 648, 813, 1176
Tru9I TTAA 3 cut(s) 648, 813, 1176
TscAI CASTG 3 cut(s) 882, 1084, 1525
TseFI GTSAC 1 cut(s) 1604
TseI GCWGC 1 cut(s) 1395
Tsp45I GTSAC 1 cut(s) 1604
TspGWI ACGGA 1 cut(s) 1623
TspRI CASTG 3 cut(s) 882, 1084, 1525
Van91I CCANNNNNTGG 2 cut(s) 43, 195
VpaK11BI GGWCC 2 cut(s) 388, 843
XapI RAATTY 5 cut(s) 290, 321, 1157, 1433, 1652
XbaI TCTAGA 2 cut(s) 317, 676
XmnI GAANNNNTTC 2 cut(s) 631, 1684
ZrmI AGTACT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.