RLG00000003474

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010777
Physical Location & Seq
Forward (+)
48873964 .. 48875697
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003474

Sequence Viewer

Length: 1734 bp
ATGGCAGAGCCAGAATCAGAGTCTTACACCAAACCCCAAAAGTCGGTCTCATCAGATCAAAACCATGAAAACCCAAGTAAGTTCTACAATCATTTTCTCTACAAAGTTGCTCTAGTTGTAGTTTTCTTCGTTATTCTCCCTCTTTTTCCATCACAAGCTCCTGAGTTCATAAACCAAAGTGTTGTCACCAGAAGCTGGGAGCTTGTTCATCTCCTCCTAGTTGGTATAGCCGTCTCTTATGGCTTATTTAGCAGACTAAATGAAAAAGTAGAGAAAGAAAACAACACGAAATTCGATAATGCTCATTCCTATGTGTCTAGAATTCTTGAGGTGTCCTCAGTTTTTGATGATGAGGCAGAAACCTCACCTGGACCTGATGAAAACAAGGTTCAGGCATGGAGTAGTCAGTATTTTAGGAATGAACCTGTGGTGGTTGTGGCTCAAGAACACTCTGTTCTTGATGAGCAGAGAGATAGCAGTTCAATACTTGGTGAAAAGCCATTGCTTTTGCCTGTTAGGAGCCTAAAACAACGTGTTCCTGACCAGGATAACATAGAGTCCATTAATGAATCTAGTGTTAATTCTGGTGGTGCTCTGAGTAGGTCTAATTCTAGGTCTGGTTCAAGAAGGTTTTCGAGCAAATCGAGTAAGGCTAAAGCTGGTGAAATTGGGGGTCTAGATCATCAAGAATTGGAGGAGAAATTGAAGGAGAGTGTTGTGCTTCCTTCTCCAATTCCGTGGAGATCAAGATCAGGAAGGTTGGAAGTAAAGGAGGATCTTGTTAGTAGTACTCCAATGGAGGAAGCTGAATTTAACCGCCTAGATCCTCGGGGTGTTTCGAGGTCCCAAAGCTCTCGTTCATCTCGATCCGAGTCAGTGCCTTCATCACCAAAGCTGTCTCCTTCGACATCACTTTTGTCCCCAAAGAAGTTATCTCCTGCACCTTCATTGTCATCAGAAGCTCAAGCCAAGAGTGTTGAGGATGTGGGGAGGAAGAAGAGCTTTTACAAGACTTCTATTCCCCCACCTCCTCCGCCCCCACCGATGTTTTATAAATCATCATCATTGAGGCCAAGCAGTGATGAGGTTTCGCATGAGAAGGATTTGAGGAGAAGCTTCACTAATGAAGCAAAGAACTTGAATAGAAGCAATGTAGAATTTATGATGGGCAGAGTTAATTCAGGGGTTGAAACAAAGCATAGGCTGAGCCATGTTGATGGCATTTCAATGGGAAAGTCTGTCAGAACAACCAGAGCTGGAGAACAAGGATATGTAAATGGGAAGGTGGAGCAAAGCGCTAAGGAAGTTGAAGCAAATTTGGTTGAGAATGTAACAAGAAAAAGGGTGGGGTTCGATGAATCTTCATTCTGGACTGAGCAATTGAGCCATGAAAGCAGCATTCCCAACAACCCCAAGTCATCAGCTTTTCAGGAATTTTCCGAGGAAGATGAGAAGGAAAATCTTTTCAATAAGGTGCTTATGGAATCGGATGAAGAAACCGAAAGCGAGGATGACGACACTGAAGGAGGTTTTTCTCCTAAAGACATTGGAGAGAGCCCAAAGCCAAGTCCAAGGCCCTATCAACCTGTTTCTGGGAGTGCAGGTGATGGAGGGCCTGATGTGGATAAAAAGGCAGATGAGTTCATAGCTAAATTCAGGGAACAGATTCGGCTTCAGAGAATAGATTCGATCAAGAAATCGAGTGCTCAGATTAGTAAAAACTTGTCGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

578

Amino Acids

64.18

Weight (kDa)

5.72

Isoelectric Point (pI)

66.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 539 - 569 1.6e-13 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1053
AarI CACCTGC 1 cut(s) 1592
Acc36I ACCTGC 1 cut(s) 1592
AccB7I CCANNNNNTGG 1 cut(s) 195
AciI CCGC 2 cut(s) 817, 1034
AclWI GGATC 3 cut(s) 783, 818, 861
AcsI RAATTY 7 cut(s) 290, 321, 809, 1157, 1315, 1433, 1652
AcuI CTGAAG 2 cut(s) 1542, 1658
AfaI GTAC 1 cut(s) 790
AfeI AGCGCT 1 cut(s) 1297
AfiI CCNNNNNNNGG 3 cut(s) 43, 195, 1592
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 8 cut(s) 483, 624, 706, 1141, 1190, 1227, 1310, 1468
AjnI CCWGG 2 cut(s) 367, 543
Alw21I GWGCWC 2 cut(s) 595, 1708
Alw26I GTCTC 3 cut(s) 52, 238, 903
AlwI GGATC 3 cut(s) 783, 818, 861
AlwNI CAGNNNCTG 1 cut(s) 195
Ama87I CYCGRG 1 cut(s) 828
Aor51HI AGCGCT 1 cut(s) 1297
AoxI GGCC 3 cut(s) 1070, 1574, 1613
ApeKI GCWGC 1 cut(s) 1395
ApoI RAATTY 7 cut(s) 290, 321, 809, 1157, 1315, 1433, 1652
ArsI GACNNNNNNTTYG 2 cut(s) 898, 930
AseI ATTAAT 1 cut(s) 564
Asp700I GAANNNNTTC 3 cut(s) 631, 1665, 1684
AspLEI GCGC 1 cut(s) 1298
AspS9I GGNCC 4 cut(s) 371, 843, 1575, 1613
AsuHPI GGTGA 6 cut(s) 178, 357, 503, 674, 879, 1616
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 2 cut(s) 371, 843
BanII GRGCYC 1 cut(s) 1559
BarI GAAGNNNNNNTAC 2 cut(s) 989, 1021
Bbv12I GWGCWC 2 cut(s) 595, 1708
BbvI GCAGC 1 cut(s) 1407
BccI CCATC 4 cut(s) 157, 1159, 1211, 1601
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 2 cut(s) 369, 545
BcoDI GTCTC 3 cut(s) 52, 238, 903
BfaI CTAG 7 cut(s) 113, 218, 318, 573, 612, 677, 821
BfoI RGCGCY 1 cut(s) 1299
BfuAI ACCTGC 1 cut(s) 1592
BisI GCNGC 1 cut(s) 1396
BlpI GCTNAGC 1 cut(s) 1205
BlsI GCNGC 1 cut(s) 1397
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 2 cut(s) 369, 545
Bme18I GGWCC 2 cut(s) 371, 843
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 4 cut(s) 371, 843, 1575, 1613
BmiI GGNNCC 2 cut(s) 521, 845
BmrFI CCNGG 2 cut(s) 369, 545
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 1 cut(s) 1278
Bpu10I CCTNAGC 1 cut(s) 1299
Bpu1102I GCTNAGC 1 cut(s) 1205
BpuEI CTTGAG 3 cut(s) 347, 426, 948
BsaBI GATNNNNATC 1 cut(s) 748
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 4 cut(s) 737, 827, 1440, 1571
BsaXI ACNNNNNCTCC 2 cut(s) 1588, 1618
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 195, 1592
Bse3DI GCAATG 2 cut(s) 500, 1156
Bse8I GATNNNNATC 1 cut(s) 748
BseBI CCWGG 2 cut(s) 369, 545
BseDI CCNNGG 4 cut(s) 737, 827, 1440, 1571
BseGI GGATG 3 cut(s) 988, 1495, 1516
BseJI GATNNNNATC 1 cut(s) 748
BseLI CCNNNNNNNGG 3 cut(s) 43, 195, 1592
BseMI GCAATG 2 cut(s) 500, 1156
BseMII CTCAG 6 cut(s) 153, 351, 587, 1196, 1365, 1721
BseRI GAGGAG 4 cut(s) 203, 710, 1020, 1123
BseXI GCAGC 1 cut(s) 1407
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 2 cut(s) 924, 1620
BshFI GGCC 3 cut(s) 1072, 1576, 1615
BsiHKAI GWGCWC 2 cut(s) 595, 1708
BsiHKCI CYCGRG 1 cut(s) 828
BslFI GGGAC 2 cut(s) 829, 904
BslI CCNNNNNNNGG 3 cut(s) 43, 195, 1592
BsmAI GTCTC 3 cut(s) 52, 238, 903
BsmBI CGTCTC 1 cut(s) 238
BsmFI GGGAC 2 cut(s) 829, 904
BsmI GAATGC 1 cut(s) 1398
BsnI GGCC 3 cut(s) 1072, 1576, 1615
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 828
Bsp1286I GDGCHC 3 cut(s) 595, 1559, 1708
Bsp143I GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
Bsp1720I GCTNAGC 1 cut(s) 1205
BspACI CCGC 2 cut(s) 817, 1034
BspANI GGCC 3 cut(s) 1072, 1576, 1615
BspCNI CTCAG 6 cut(s) 154, 350, 588, 1197, 1366, 1720
BspLI GGNNCC 2 cut(s) 521, 845
BspMI ACCTGC 1 cut(s) 1592
BspPI GGATC 3 cut(s) 783, 818, 861
BspQI GCTCTTC 1 cut(s) 992
BspTNI GGTCTC 1 cut(s) 52
BsrDI GCAATG 2 cut(s) 500, 1156
BssECI CCNNGG 4 cut(s) 737, 827, 1440, 1571
BssMI GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
BssT1I CCWWGG 1 cut(s) 1571
Bst2UI CCWGG 2 cut(s) 369, 545
Bst6I CTCTTC 1 cut(s) 992
BstDEI CTNAG 7 cut(s) 162, 337, 596, 1205, 1299, 1374, 1707
BstDSI CCRYGG 1 cut(s) 737
BstF5I GGATG 3 cut(s) 988, 1495, 1516
BstH2I RGCGCY 1 cut(s) 1299
BstHHI GCGC 1 cut(s) 1298
BstKTI GATC 8 cut(s) 58, 682, 746, 752, 778, 826, 869, 1692
BstMAI GTCTC 3 cut(s) 52, 238, 903
BstMBI GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
BstMWI GCNNNNNNNGC 2 cut(s) 249, 1392
BstNI CCWGG 2 cut(s) 369, 545
BstSCI CCNGG 2 cut(s) 367, 543
BstV1I GCAGC 1 cut(s) 1407
BstX2I RGATCY 2 cut(s) 775, 823
BstXI CCANNNNNNTGG 2 cut(s) 738, 1217
BstYI RGATCY 2 cut(s) 775, 823
BsuRI GGCC 3 cut(s) 1072, 1576, 1615
BtgI CCRYGG 1 cut(s) 737
BtsCI GGATG 3 cut(s) 988, 1495, 1516
BtsI GCAGTG 1 cut(s) 1084
BtsIMutI CAGTG 3 cut(s) 882, 1084, 1518
BveI ACCTGC 1 cut(s) 1592
CaiI CAGNNNCTG 1 cut(s) 195
CfoI GCGC 1 cut(s) 1298
Cfr13I GGNCC 4 cut(s) 371, 843, 1575, 1613
Csp6I GTAC 1 cut(s) 789
CviAII CATG 5 cut(s) 65, 396, 1094, 1211, 1388
CviQI GTAC 1 cut(s) 789
DdeI CTNAG 7 cut(s) 162, 337, 596, 1205, 1299, 1374, 1707
DpnI GATC 8 cut(s) 57, 681, 745, 751, 777, 825, 868, 1691
DpnII GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
Eam1104I CTCTTC 1 cut(s) 992
EarI CTCTTC 1 cut(s) 992
EciI GGCGGA 1 cut(s) 1023
Eco130I CCWWGG 1 cut(s) 1571
Eco24I GRGCYC 1 cut(s) 1559
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 2 cut(s) 371, 843
Eco47III AGCGCT 1 cut(s) 1297
Eco57I CTGAAG 2 cut(s) 1542, 1658
Eco88I CYCGRG 1 cut(s) 828
EcoO109I RGGNCCY 3 cut(s) 843, 1575, 1613
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 367, 543
EcoT14I CCWWGG 1 cut(s) 1571
EcoT38I GRGCYC 1 cut(s) 1559
ErhI CCWWGG 1 cut(s) 1571
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 5 cut(s) 68, 399, 1097, 1214, 1391
FalI AAGNNNNNCTT 2 cut(s) 986, 1018
FaqI GGGAC 2 cut(s) 829, 904
FatI CATG 5 cut(s) 64, 395, 1093, 1210, 1387
Fnu4HI GCNGC 1 cut(s) 1396
FokI GGATG 3 cut(s) 995, 1502, 1523
FriOI GRGCYC 1 cut(s) 1559
Fsp4HI GCNGC 1 cut(s) 1396
FspBI CTAG 7 cut(s) 113, 218, 318, 573, 612, 677, 821
GlaI GCGC 1 cut(s) 1297
GluI GCNGC 1 cut(s) 1396
GsaI CCCAGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 1278
HaeII RGCGCY 1 cut(s) 1299
HaeIII GGCC 3 cut(s) 1072, 1576, 1615
HhaI GCGC 1 cut(s) 1298
Hin1II CATG 5 cut(s) 68, 399, 1097, 1214, 1391
Hin6I GCGC 1 cut(s) 1296
HinP1I GCGC 1 cut(s) 1296
HindIII AAGCTT 1 cut(s) 1114
HinfI GANTC 9 cut(s) 14, 20, 557, 569, 872, 1358, 1484, 1666, 1685
HphI GGTGA 6 cut(s) 178, 357, 503, 674, 879, 1616
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 2 cut(s) 941, 1601
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 1392
HpyF3I CTNAG 7 cut(s) 162, 337, 596, 1205, 1299, 1374, 1707
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 5 cut(s) 68, 399, 1097, 1214, 1391
HspAI GCGC 1 cut(s) 1296
Kzo9I GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
LguI GCTCTTC 1 cut(s) 992
LmnI GCTCC 4 cut(s) 163, 199, 519, 1288
Lsp1109I GCAGC 1 cut(s) 1407
MaeI CTAG 7 cut(s) 113, 218, 318, 573, 612, 677, 821
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 2 cut(s) 184, 1330
MalI GATC 8 cut(s) 57, 681, 745, 751, 777, 825, 868, 1691
MboI GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
MboII GAAGA 6 cut(s) 118, 1006, 1009, 1353, 1457, 1505
MfeI CAATTG 1 cut(s) 1379
MflI RGATCY 2 cut(s) 775, 823
MhlI GDGCHC 3 cut(s) 595, 1559, 1708
MlyI GAGTC 3 cut(s) 29, 566, 881
MmeI TCCRAC 1 cut(s) 741
MroXI GAANNNNTTC 3 cut(s) 631, 1665, 1684
MseI TTAA 4 cut(s) 564, 579, 813, 1176
MslI CAYNNNNRTG 3 cut(s) 309, 1215, 1226
MspR9I CCNGG 2 cut(s) 369, 545
MunI CAATTG 1 cut(s) 1379
Mva1269I GAATGC 1 cut(s) 1398
MvaI CCWGG 2 cut(s) 369, 545
MwoI GCNNNNNNNGC 2 cut(s) 249, 1392
NdeII GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
NlaIII CATG 5 cut(s) 68, 399, 1097, 1214, 1391
NlaIV GGNNCC 2 cut(s) 521, 845
NmuCI GTSAC 1 cut(s) 184
PaqCI CACCTGC 1 cut(s) 1592
PciSI GCTCTTC 1 cut(s) 992
PcsI WCGNNNNNNNCGW 2 cut(s) 641, 862
PctI GAATGC 1 cut(s) 1398
PdmI GAANNNNTTC 3 cut(s) 631, 1665, 1684
PfeI GAWTC 6 cut(s) 14, 569, 1358, 1484, 1666, 1685
PflMI CCANNNNNTGG 1 cut(s) 195
PkrI GCNGC 1 cut(s) 1397
PleI GAGTC 3 cut(s) 28, 565, 880
PpsI GAGTC 3 cut(s) 28, 565, 880
PpuMI RGGWCCY 1 cut(s) 843
PshBI ATTAAT 1 cut(s) 564
PsiI TTATAA 1 cut(s) 1053
Psp5II RGGWCCY 1 cut(s) 843
Psp6I CCWGG 2 cut(s) 367, 543
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 2 cut(s) 367, 543
PspN4I GGNNCC 2 cut(s) 521, 845
PspPI GGNCC 4 cut(s) 371, 843, 1575, 1613
PspPPI RGGWCCY 1 cut(s) 843
PstNI CAGNNNCTG 1 cut(s) 195
PsuI RGATCY 2 cut(s) 775, 823
RsaI GTAC 1 cut(s) 790
RsaNI GTAC 1 cut(s) 789
RseI CAYNNNNRTG 3 cut(s) 309, 1215, 1226
SapI GCTCTTC 1 cut(s) 992
SaqAI TTAA 4 cut(s) 564, 579, 813, 1176
SatI GCNGC 1 cut(s) 1396
Sau3AI GATC 8 cut(s) 55, 679, 743, 749, 775, 823, 866, 1689
Sau96I GGNCC 4 cut(s) 371, 843, 1575, 1613
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 3 cut(s) 29, 566, 881
ScrFI CCNGG 2 cut(s) 369, 545
SduI GDGCHC 3 cut(s) 595, 1559, 1708
SinI GGWCC 2 cut(s) 371, 843
SmiMI CAYNNNNRTG 3 cut(s) 309, 1215, 1226
SmlI CTYRAG 3 cut(s) 326, 441, 963
SmoI CTYRAG 3 cut(s) 326, 441, 963
SsiI CCGC 2 cut(s) 817, 1034
SspMI CTAG 7 cut(s) 113, 218, 318, 573, 612, 677, 821
StyD4I CCNGG 2 cut(s) 367, 543
StyI CCWWGG 1 cut(s) 1571
TaiI ACGT 1 cut(s) 535
TaqII GACCGA 1 cut(s) 34
TatI WGTACW 1 cut(s) 788
TfiI GAWTC 6 cut(s) 14, 569, 1358, 1484, 1666, 1685
Tru1I TTAA 4 cut(s) 564, 579, 813, 1176
Tru9I TTAA 4 cut(s) 564, 579, 813, 1176
TscAI CASTG 3 cut(s) 882, 1084, 1525
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 1395
Tsp45I GTSAC 1 cut(s) 184
TspGWI ACGGA 1 cut(s) 726
TspRI CASTG 3 cut(s) 882, 1084, 1525
Van91I CCANNNNNTGG 1 cut(s) 195
VpaK11BI GGWCC 2 cut(s) 371, 843
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 7 cut(s) 290, 321, 809, 1157, 1315, 1433, 1652
XbaI TCTAGA 2 cut(s) 317, 676
XmnI GAANNNNTTC 3 cut(s) 631, 1665, 1684
XspI CTAG 7 cut(s) 113, 218, 318, 573, 612, 677, 821
ZrmI AGTACT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.