Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0005759

Physical Location & Seq

Genomic Coordinates Reverse (-)

15866940 .. 15872298

Gene Structure 5359 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g24520.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGAACGACGATAGCCTCCAGCAAGATTTGGACCCCCAAAGCGACCAGAGCTCCGACTACCTCCCACCTCAGCCCCACCCTCTCCCCTCCCAACCTCACCCCCACCCTCGCCGGCCACGTGGCTTCGCCGCCACAGCCGCTGCCGGCTCTGCGGGGCCCATCTCCCCCTCCACCAAGGGAAGGAGAGAGAGGGAGAAAGAAAAGGAGCGGACTAAGCTTCGCGAACGCCTCCGCCGATCAATCACCAGCCGCATGCTCGCCGGACTTCGCCAGTACGGCAACTTCCCCCTGCCGGCGCGTGCAGATATGAACGATGTACTCGCCGCTCTAGCGCGTGAGGCCGGCTGGGTCGTCGAGCCTGACGGCACCACTTATCGCCAGTCTCCGCCTCAAATTGGAGGAGCTTATTCCATGAATTTGAGGTCAGTGGAGACTCCAGTGTCGTTAGAGTGTCATCCACAATCAGTAGCTAGAATTGATGAGAGCTTATCTCCGGCGTCGCTCGATTCCGTGGTGATTGCTGAGAAGAATGACAGGTTTTCGAGTGGAAGTCCGGCGATGAACTCTGTTAATTGCTTAGAGGCTGATCAGCTGATGCAGCACATTCCTTCTGCCACCAGTTTCGCGCCTGCTCCATATGTCCCTCTCTATGTTACACTAGTAGCAACTGGTTTCGTCAACAATTACTGCCAGTTGGTTGATCCTGATGGGTTAAGGCAGGAGCTTACTCATTTGCAGTCTCTAAATGTGGATGGTGTTGTTGTGGATTGTTGGTGGGGTATTGTCGAATGCTGGAGCCCCCATAAATATGATTGGTCGGGTTATAGGGGTCTCTTTAATATTGTACGCGAGTTCAAGCTTAAATTACAGGTTGTAATGGCTTTTCATGAGTATGGAAGAAGGGAGTCTGGTGACGCCCTTATTCCTCTTCCGAAGTGGATTTTAGACATTGGTAAAGAAAACCAGGACATATTCTTTACTGATCGAGAAGGAAGGAGGAATACTGAATGTCTATCTTGGGGCGTCGACAAAGAGAGAGTTTTGCATGGAAGAACTGGTGTCGAGGTCTATTTTGATTTGATGAGAAGTTTCCGTACCGAGTTTGATGACTTGTTTGTGGAGGGTCTTATTTCTGCTGTGGAGATTGGACTTGGAGCTTCTGGGGAGTTAAAGTATCCTTCCTTTTCAGAAAGGATGGGATGGAGATACCCTGGTGTCGGTGAGTTTCAGTGTTATGATAGATACCTACAACAAAGCCTGCAGAAAGCAGCAAAATTGCGTGGGCACTCGTTCTGGGCTAGAGGACCAGATAATGCTGGTGAATACAATTCTCGGCCACATGAAACTGGCTTCTTCTGTGAGCGTGGTGATTATGATAGTTACTATGGACGCTTTTTCCTACATTGGTATGCCAAGACACTAATTGACCATGCAGACAATGTCCTATCTCTTGCAAGCCTTGCCTTTGATGAAACACAAGTAATTGTCAAGGTTCCTTCTGTTTATTGGTGGTACAAAACTCCTAGCCATGCAGCAGAGCTAACATCTGGGTACTATAATCCTAAAAACCAAGATGGGTACTCTCTGGTGTTTGAAGTTCTGAAGAAACATTCGGTGACGGTTAAGTTTGTTTGCTCAGCACCAAATGATGGTGATGAAGCATTGGGCGATCCAGAAGGTTTGAGTTGGCAGGTTTTAAATTTAGCTTGGGATCGAGGATTGACTGTGGCTGGGGAAAATGCACTGTCATGCTATGATAGAGAAGGGTGCATGAGAATTGTTGAATTGGTGAAGCCAAGAAATGATCCTGATCGCCACCATTTCACATTCTTTGTATATCAGCAGCCATCTCCTCTAGTTCAAGGGACAGTTTGCTTTTCGGAGTTGGACTTCTTCATCAAGTGCATGCATGGTGACGTCGCTGGGAATCTAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

BES1_N Glyco_hydro_14

Protein Analysis

646

Amino Acids

72.82

Weight (kDa)

5.63

Isoelectric Point (pI)

46.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BES1_N	PF05687	59 - 191	1.4e-42	BES1/BZR1 plant transcription factor, N-terminal
Glyco_hydro_14	PF01373	231 - 414	9.3e-53	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005759)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45300 AT5G45300 AT5G45300
fragaria_vesca	FvH4_5g24520 FvH4_5g24520 FvH4_5g24520 FvH4_5g24520 FvH4_5g24520
malus_domestica	MD06G1012600.v1.1
prunus_persica	Prupe.5G013400_v2.0.a1 Prupe.5G013400_v2.0.a1 Prupe.5G013400_v2.0.a1 Prupe.5G013400_v2.0.a1 Prupe.5G013400_v2.0.a1
pyrus_communis	pycom06g01050
rosa_chinensis	RchiOBHm_Chr7g0215001
rosa_laevigata	RLG00000002702
rosa_multiflora	Rmu_co8013204.1_g000001 Rmu_sc0004525.1_g000013 Rmu_ssc0000134.1_g000045
rosa_roxburghii	Rroxscaffold_3G00244800
rosa_rugosa	Rorug07G0149100
rosa_samantha	Rh7AG286200 Rh7BG277900 Rh7CG305300 Rh7CG305400 Rh7DG289500
rosa_wichuraiana	Rw7G024410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	439
AatII	GACGTC	1 cut(s)	1920
Acc36I	ACCTGC	1 cut(s)	1681
AccB1I	GGYRCC	1 cut(s)	365
AccB7I	CCANNNNNTGG	1 cut(s)	1649
AccBSI	CCGCTC	2 cut(s)	208, 326
AccI	GTMKAC	1 cut(s)	1026
AccII	CGCG	5 cut(s)	222, 298, 334, 626, 849
AciI	CCGC	8 cut(s)	129, 138, 152, 208, 232, 250, 324, 386
AclWI	GGATC	4 cut(s)	695, 1664, 1719, 1799
AcoI	YGGCCR	2 cut(s)	113, 1334
AcsI	RAATTY	2 cut(s)	415, 1699
AcuI	CTGAAG	1 cut(s)	1622
AcvI	CACGTG	1 cut(s)	119
AcyI	GRCGYC	4 cut(s)	497, 915, 1023, 1917
AfaI	GTAC	7 cut(s)	275, 318, 846, 1096, 1514, 1553, 1580
AfiI	CCNNNNNNNGG	5 cut(s)	180, 292, 395, 1217, 1649
AgsI	TTSAA	4 cut(s)	856, 1595, 1784, 1862
AhlI	ACTAGT	1 cut(s)	658
AjnI	CCWGG	2 cut(s)	963, 1210
Alw21I	GWGCWC	1 cut(s)	53
Alw26I	GTCTC	4 cut(s)	387, 425, 744, 836
AlwI	GGATC	4 cut(s)	695, 1664, 1719, 1799
AlwNI	CAGNNNCTG	1 cut(s)	140
AoxI	GGCC	4 cut(s)	113, 155, 339, 1334
ApaI	GGGCCC	1 cut(s)	159
ApeKI	GCWGC	5 cut(s)	140, 598, 1268, 1532, 1843
ApoI	RAATTY	2 cut(s)	415, 1699
AspLEI	GCGC	3 cut(s)	298, 334, 628
AspS9I	GGNCC	4 cut(s)	31, 155, 156, 1304
AvaII	GGWCC	2 cut(s)	31, 1304
BaeGI	GKGCMC	2 cut(s)	159, 1287
BaeI	ACNNNNGTAYC	2 cut(s)	1198, 1231
BanI	GGYRCC	1 cut(s)	365
BanII	GRGCYC	3 cut(s)	53, 159, 800
BarI	GAAGNNNNNNTAC	6 cut(s)	266, 298, 985, 1017, 1078, 1110
BbrPI	CACGTG	1 cut(s)	119
Bbv12I	GWGCWC	1 cut(s)	53
BbvCI	CCTCAGC	1 cut(s)	69
BbvI	GCAGC	5 cut(s)	127, 610, 1280, 1544, 1855
BccI	CCATC	8 cut(s)	167, 701, 746, 1189, 1194, 1568, 1643, 1855
BceAI	ACGGC	2 cut(s)	292, 379
BciT130I	CCWGG	2 cut(s)	965, 1212
BciVI	GTATCC	1 cut(s)	1185
BclI	TGATCA	1 cut(s)	586
BcoDI	GTCTC	4 cut(s)	387, 425, 744, 836
BcuI	ACTAGT	1 cut(s)	658
BfaI	CTAG	7 cut(s)	329, 471, 659, 1299, 1524, 1856, 1931
BfmI	CTRYAG	1 cut(s)	1259
BfuAI	ACCTGC	1 cut(s)	1681
BfuI	GTATCC	1 cut(s)	1185
BglI	GCCNNNNNGGC	1 cut(s)	276
BisI	GCNGC	9 cut(s)	129, 138, 141, 250, 324, 599, 1269, 1533, 1844
BlpI	GCTNAGC	1 cut(s)	1636
BlsI	GCNGC	9 cut(s)	130, 139, 142, 251, 325, 600, 1270, 1534, 1845
Bme1390I	CCNGG	2 cut(s)	965, 1212
Bme18I	GGWCC	2 cut(s)	31, 1304
BmgT120I	GGNCC	4 cut(s)	31, 155, 156, 1304
BmiI	GGNNCC	6 cut(s)	33, 156, 157, 367, 797, 1494
BmrFI	CCNGG	2 cut(s)	965, 1212
BmsI	GCATC	1 cut(s)	585
BplI	GAGNNNNNCTC	2 cut(s)	475, 507
BpmI	CTGGAG	2 cut(s)	420, 814
Bpu10I	CCTNAGC	1 cut(s)	69
Bpu1102I	GCTNAGC	1 cut(s)	1636
BsaAI	YACGTR	1 cut(s)	119
BsaBI	GATNNNNATC	1 cut(s)	1809
BsaHI	GRCGYC	4 cut(s)	497, 915, 1023, 1917
BsaI	GGTCTC	1 cut(s)	836
BsaJI	CCNNGG	3 cut(s)	174, 510, 1210
BsaXI	ACNNNNNCTCC	4 cut(s)	35, 65, 1157, 1187
Bsc4I	CCNNNNNNNGG	5 cut(s)	180, 292, 395, 1217, 1649
Bse118I	RCCGGY	4 cut(s)	111, 143, 292, 341
Bse1I	ACTGG	8 cut(s)	271, 379, 437, 618, 673, 691, 1060, 1351
Bse8I	GATNNNNATC	1 cut(s)	1809
BseBI	CCWGG	2 cut(s)	965, 1212
BseDI	CCNNGG	3 cut(s)	174, 510, 1210
BseGI	GGATG	4 cut(s)	454, 757, 1200, 1205
BseJI	GATNNNNATC	1 cut(s)	1809
BseLI	CCNNNNNNNGG	5 cut(s)	180, 292, 395, 1217, 1649
BseMII	CTCAG	3 cut(s)	83, 513, 1650
BseNI	ACTGG	8 cut(s)	271, 379, 437, 618, 673, 691, 1060, 1351
BseRI	GAGGAG	2 cut(s)	414, 1842
BseSI	GKGCMC	2 cut(s)	159, 1287
BseXI	GCAGC	5 cut(s)	127, 610, 1280, 1544, 1855
BseYI	CCCAGC	3 cut(s)	345, 1730, 1922
BsgI	GTGCAG	1 cut(s)	321
Bsh1236I	CGCG	5 cut(s)	222, 298, 334, 626, 849
BshFI	GGCC	4 cut(s)	115, 157, 341, 1336
BshNI	GGYRCC	1 cut(s)	365
BsiHKAI	GWGCWC	1 cut(s)	53
BsiSI	CCGG	7 cut(s)	112, 144, 261, 293, 342, 494, 554
BslFI	GGGAC	2 cut(s)	626, 1879
BslI	CCNNNNNNNGG	5 cut(s)	180, 292, 395, 1217, 1649
BsmAI	GTCTC	4 cut(s)	387, 425, 744, 836
BsmFI	GGGAC	2 cut(s)	626, 1879
BsmI	GAATGC	1 cut(s)	794
BsnI	GGCC	4 cut(s)	115, 157, 341, 1336
Bso31I	GGTCTC	1 cut(s)	836
Bsp120I	GGGCCC	1 cut(s)	155
Bsp1286I	GDGCHC	4 cut(s)	53, 159, 800, 1287
Bsp143I	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
Bsp1720I	GCTNAGC	1 cut(s)	1636
Bsp68I	TCGCGA	1 cut(s)	222
BspACI	CCGC	8 cut(s)	129, 138, 152, 208, 232, 250, 324, 386
BspANI	GGCC	4 cut(s)	115, 157, 341, 1336
BspCNI	CTCAG	3 cut(s)	82, 514, 1649
BspFNI	CGCG	5 cut(s)	222, 298, 334, 626, 849
BspHI	TCATGA	1 cut(s)	886
BspLI	GGNNCC	6 cut(s)	33, 156, 157, 367, 797, 1494
BspMAI	CTGCAG	1 cut(s)	1263
BspMI	ACCTGC	1 cut(s)	1681
BspPI	GGATC	4 cut(s)	695, 1664, 1719, 1799
BspT107I	GGYRCC	1 cut(s)	365
BspTNI	GGTCTC	1 cut(s)	836
BsrBI	CCGCTC	2 cut(s)	208, 326
BsrFI	RCCGGY	4 cut(s)	111, 143, 292, 341
BsrI	ACTGG	8 cut(s)	271, 379, 437, 618, 673, 691, 1060, 1351
BssAI	RCCGGY	4 cut(s)	111, 143, 292, 341
BssECI	CCNNGG	3 cut(s)	174, 510, 1210
BssMI	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
BssNI	GRCGYC	4 cut(s)	497, 915, 1023, 1917
BssT1I	CCWWGG	1 cut(s)	174
Bst2UI	CCWGG	2 cut(s)	965, 1212
Bst4CI	ACNGT	4 cut(s)	1621, 1726, 1746, 1870
Bst6I	CTCTTC	1 cut(s)	933
BstACI	GRCGYC	4 cut(s)	497, 915, 1023, 1917
BstAPI	GCANNNNNTGC	1 cut(s)	1460
BstBAI	YACGTR	1 cut(s)	119
BstDEI	CTNAG	5 cut(s)	69, 213, 522, 577, 1636
BstDSI	CCRYGG	1 cut(s)	510
BstF5I	GGATG	4 cut(s)	454, 757, 1200, 1205
BstFNI	CGCG	5 cut(s)	222, 298, 334, 626, 849
BstHHI	GCGC	3 cut(s)	298, 334, 628
BstKTI	GATC	8 cut(s)	239, 589, 703, 985, 1672, 1714, 1807, 1813
BstMAI	GTCTC	4 cut(s)	387, 425, 744, 836
BstMBI	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
BstNI	CCWGG	2 cut(s)	965, 1212
BstNSI	RCATGY	2 cut(s)	256, 1909
BstSCI	CCNGG	2 cut(s)	963, 1210
BstSFI	CTRYAG	1 cut(s)	1259
BstSLI	GKGCMC	2 cut(s)	159, 1287
BstUI	CGCG	5 cut(s)	222, 298, 334, 626, 849
BstV1I	GCAGC	5 cut(s)	127, 610, 1280, 1544, 1855
BsuI	GTATCC	1 cut(s)	1185
BsuRI	GGCC	4 cut(s)	115, 157, 341, 1336
BtgI	CCRYGG	1 cut(s)	510
BtgZI	GCGATG	1 cut(s)	572
BtsCI	GGATG	4 cut(s)	454, 757, 1200, 1205
BtsIMutI	CAGTG	4 cut(s)	432, 444, 1235, 1742
BtuMI	TCGCGA	1 cut(s)	222
BveI	ACCTGC	1 cut(s)	1681
CaiI	CAGNNNCTG	1 cut(s)	140
CciI	TCATGA	1 cut(s)	886
CfoI	GCGC	3 cut(s)	298, 334, 628
Cfr10I	RCCGGY	4 cut(s)	111, 143, 292, 341
Cfr13I	GGNCC	4 cut(s)	31, 155, 156, 1304
CseI	GACGC	4 cut(s)	486, 923, 1012, 1398
Csp6I	GTAC	7 cut(s)	274, 317, 845, 1095, 1513, 1552, 1579
CviQI	GTAC	7 cut(s)	274, 317, 845, 1095, 1513, 1552, 1579
DdeI	CTNAG	5 cut(s)	69, 213, 522, 577, 1636
DpnI	GATC	8 cut(s)	238, 588, 702, 984, 1671, 1713, 1806, 1812
DpnII	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
DraI	TTTAAA	1 cut(s)	1698
DrdI	GACNNNNNNGTC	1 cut(s)	439
DseDI	GACNNNNNNGTC	1 cut(s)	439
EaeI	YGGCCR	2 cut(s)	113, 1334
Eam1104I	CTCTTC	1 cut(s)	933
EarI	CTCTTC	1 cut(s)	933
EciI	GGCGGA	2 cut(s)	221, 375
Ecl136II	GAGCTC	1 cut(s)	51
Eco130I	CCWWGG	1 cut(s)	174
Eco24I	GRGCYC	3 cut(s)	53, 159, 800
Eco31I	GGTCTC	1 cut(s)	836
Eco47I	GGWCC	2 cut(s)	31, 1304
Eco53kI	GAGCTC	1 cut(s)	51
Eco57I	CTGAAG	1 cut(s)	1622
Eco72I	CACGTG	1 cut(s)	119
EcoICRI	GAGCTC	1 cut(s)	51
EcoO109I	RGGNCCY	1 cut(s)	155
EcoRII	CCWGG	2 cut(s)	963, 1210
EcoT14I	CCWWGG	1 cut(s)	174
EcoT22I	ATGCAT	1 cut(s)	1911
EcoT38I	GRGCYC	3 cut(s)	53, 159, 800
ErhI	CCWWGG	1 cut(s)	174
FaqI	GGGAC	2 cut(s)	626, 1879
FauI	CCCGC	1 cut(s)	145
FauNDI	CATATG	1 cut(s)	637
FbaI	TGATCA	1 cut(s)	586
FblI	GTMKAC	1 cut(s)	1026
Fnu4HI	GCNGC	9 cut(s)	129, 138, 141, 250, 324, 599, 1269, 1533, 1844
FokI	GGATG	4 cut(s)	441, 764, 1207, 1212
FriOI	GRGCYC	3 cut(s)	53, 159, 800
Fsp4HI	GCNGC	9 cut(s)	129, 138, 141, 250, 324, 599, 1269, 1533, 1844
FspBI	CTAG	7 cut(s)	329, 471, 659, 1299, 1524, 1856, 1931
GlaI	GCGC	3 cut(s)	297, 333, 627
GluI	GCNGC	9 cut(s)	129, 138, 141, 250, 324, 599, 1269, 1533, 1844
GsaI	CCCAGC	3 cut(s)	349, 1734, 1926
GsuI	CTGGAG	2 cut(s)	420, 814
HaeIII	GGCC	4 cut(s)	115, 157, 341, 1336
HapII	CCGG	7 cut(s)	112, 144, 261, 293, 342, 494, 554
HgaI	GACGC	4 cut(s)	486, 923, 1012, 1398
HhaI	GCGC	3 cut(s)	298, 334, 628
Hin1I	GRCGYC	4 cut(s)	497, 915, 1023, 1917
Hin6I	GCGC	3 cut(s)	296, 332, 626
HinP1I	GCGC	3 cut(s)	296, 332, 626
HincII	GTYRAC	2 cut(s)	679, 1027
HindII	GTYRAC	2 cut(s)	679, 1027
HindIII	AAGCTT	2 cut(s)	215, 857
HinfI	GANTC	4 cut(s)	433, 506, 905, 1927
HpaII	CCGG	7 cut(s)	112, 144, 261, 293, 342, 494, 554
Hpy166II	GTNNAC	2 cut(s)	679, 1027
Hpy188I	TCNGA	5 cut(s)	55, 933, 1189, 1602, 1882
Hpy188III	TCNNGA	6 cut(s)	221, 704, 887, 986, 1673, 1808
Hpy8I	GTNNAC	2 cut(s)	679, 1027
Hpy99I	CGWCG	5 cut(s)	11, 356, 502, 1028, 1922
HpyAV	CCTTC	9 cut(s)	174, 618, 894, 983, 987, 1188, 1506, 1670, 1757
HpyCH4III	ACNGT	4 cut(s)	1621, 1726, 1746, 1870
HpyCH4IV	ACGT	2 cut(s)	118, 1917
HpyF3I	CTNAG	5 cut(s)	69, 213, 522, 577, 1636
HpySE526I	ACGT	2 cut(s)	118, 1917
Hsp92I	GRCGYC	4 cut(s)	497, 915, 1023, 1917
HspAI	GCGC	3 cut(s)	296, 332, 626
KroI	GCCGGC	4 cut(s)	111, 143, 292, 341
KroNI	GCCGGC	4 cut(s)	113, 145, 294, 343
Ksp22I	TGATCA	1 cut(s)	586
Kzo9I	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
LmnI	GCTCC	7 cut(s)	56, 205, 401, 637, 721, 795, 1154
Lsp1109I	GCAGC	5 cut(s)	127, 610, 1280, 1544, 1855
LweI	GCATC	1 cut(s)	585
MaeI	CTAG	7 cut(s)	329, 471, 659, 1299, 1524, 1856, 1931
MaeII	ACGT	2 cut(s)	118, 1917
MaeIII	GTNAC	5 cut(s)	652, 911, 1379, 1615, 1913
MalI	GATC	8 cut(s)	238, 588, 702, 984, 1671, 1713, 1806, 1812
MbiI	CCGCTC	2 cut(s)	208, 326
MboI	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
MboII	GAAGA	7 cut(s)	538, 909, 920, 1062, 1345, 1615, 1885
MhlI	GDGCHC	4 cut(s)	53, 159, 800, 1287
MlyI	GAGTC	2 cut(s)	427, 914
MmeI	TCCRAC	2 cut(s)	78, 1866
Mph1103I	ATGCAT	1 cut(s)	1911
MroNI	GCCGGC	4 cut(s)	111, 143, 292, 341
MseI	TTAA	7 cut(s)	570, 713, 837, 861, 1169, 1623, 1697
MslI	CAYNNNNRTG	4 cut(s)	807, 891, 1407, 1747
MspA1I	CMGCKG	2 cut(s)	140, 592
MspI	CCGG	7 cut(s)	112, 144, 261, 293, 342, 494, 554
MspR9I	CCNGG	2 cut(s)	965, 1212
Mva1269I	GAATGC	1 cut(s)	794
MvaI	CCWGG	2 cut(s)	965, 1212
MvnI	CGCG	5 cut(s)	222, 298, 334, 626, 849
NaeI	GCCGGC	4 cut(s)	113, 145, 294, 343
NdeI	CATATG	1 cut(s)	637
NdeII	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
NgoMIV	GCCGGC	4 cut(s)	111, 143, 292, 341
NlaIV	GGNNCC	6 cut(s)	33, 156, 157, 367, 797, 1494
NmeAIII	GCCGAG	1 cut(s)	1312
NmuCI	GTSAC	3 cut(s)	911, 1615, 1913
NruI	TCGCGA	1 cut(s)	222
NsiI	ATGCAT	1 cut(s)	1911
NspI	RCATGY	2 cut(s)	256, 1909
PaeI	GCATGC	2 cut(s)	256, 1909
PagI	TCATGA	1 cut(s)	886
PcsI	WCGNNNNNNNCGW	2 cut(s)	115, 232
PctI	GAATGC	1 cut(s)	794
PdiI	GCCGGC	4 cut(s)	113, 145, 294, 343
PfeI	GAWTC	2 cut(s)	506, 1927
PflFI	GACNNNGTC	1 cut(s)	1439
PflMI	CCANNNNNTGG	1 cut(s)	1649
PkrI	GCNGC	9 cut(s)	130, 139, 142, 251, 325, 600, 1270, 1534, 1845
PleI	GAGTC	2 cut(s)	427, 913
PmaCI	CACGTG	1 cut(s)	119
PmlI	CACGTG	1 cut(s)	119
PpsI	GAGTC	2 cut(s)	427, 913
Ppu21I	YACGTR	1 cut(s)	119
Psp124BI	GAGCTC	1 cut(s)	53
Psp6I	CCWGG	2 cut(s)	963, 1210
PspCI	CACGTG	1 cut(s)	119
PspFI	CCCAGC	3 cut(s)	345, 1730, 1922
PspGI	CCWGG	2 cut(s)	963, 1210
PspN4I	GGNNCC	6 cut(s)	33, 156, 157, 367, 797, 1494
PspOMI	GGGCCC	1 cut(s)	155
PspPI	GGNCC	4 cut(s)	31, 155, 156, 1304
PstI	CTGCAG	1 cut(s)	1263
PstNI	CAGNNNCTG	1 cut(s)	140
PsyI	GACNNNGTC	1 cut(s)	1439
PvuII	CAGCTG	1 cut(s)	592
RruI	TCGCGA	1 cut(s)	222
RsaI	GTAC	7 cut(s)	275, 318, 846, 1096, 1514, 1553, 1580
RsaNI	GTAC	7 cut(s)	274, 317, 845, 1095, 1513, 1552, 1579
RseI	CAYNNNNRTG	4 cut(s)	807, 891, 1407, 1747
SacI	GAGCTC	1 cut(s)	53
SalI	GTCGAC	1 cut(s)	1025
SaqAI	TTAA	7 cut(s)	570, 713, 837, 861, 1169, 1623, 1697
SatI	GCNGC	9 cut(s)	129, 138, 141, 250, 324, 599, 1269, 1533, 1844
Sau3AI	GATC	8 cut(s)	236, 586, 700, 982, 1669, 1711, 1804, 1810
Sau96I	GGNCC	4 cut(s)	31, 155, 156, 1304
SchI	GAGTC	2 cut(s)	427, 914
ScrFI	CCNGG	2 cut(s)	965, 1212
SduI	GDGCHC	4 cut(s)	53, 159, 800, 1287
SfaNI	GCATC	1 cut(s)	585
SfcI	CTRYAG	1 cut(s)	1259
SinI	GGWCC	2 cut(s)	31, 1304
SmiMI	CAYNNNNRTG	4 cut(s)	807, 891, 1407, 1747
SpeI	ACTAGT	1 cut(s)	658
SphI	GCATGC	2 cut(s)	256, 1909
SsiI	CCGC	8 cut(s)	129, 138, 152, 208, 232, 250, 324, 386
SspI	AATATT	1 cut(s)	841
SspMI	CTAG	7 cut(s)	329, 471, 659, 1299, 1524, 1856, 1931
SstI	GAGCTC	1 cut(s)	53
StyD4I	CCNGG	2 cut(s)	963, 1210
StyI	CCWWGG	1 cut(s)	174
TaaI	ACNGT	4 cut(s)	1621, 1726, 1746, 1870
TaiI	ACGT	2 cut(s)	121, 1920
TaqI	TCGA	8 cut(s)	354, 504, 542, 786, 985, 1026, 1062, 1714
TatI	WGTACW	1 cut(s)	316
TauI	GCSGC	4 cut(s)	131, 140, 252, 326
TfiI	GAWTC	2 cut(s)	506, 1927
Tru1I	TTAA	7 cut(s)	570, 713, 837, 861, 1169, 1623, 1697
Tru9I	TTAA	7 cut(s)	570, 713, 837, 861, 1169, 1623, 1697
TscAI	CASTG	4 cut(s)	432, 444, 1235, 1749
TseFI	GTSAC	3 cut(s)	911, 1615, 1913
TseI	GCWGC	5 cut(s)	140, 598, 1268, 1532, 1843
Tsp45I	GTSAC	3 cut(s)	911, 1615, 1913
TspDTI	ATGAA	9 cut(s)	17, 323, 428, 575, 875, 1356, 1485, 1671, 1885
TspGWI	ACGGA	2 cut(s)	499, 1082
TspRI	CASTG	4 cut(s)	432, 444, 1235, 1749
Tth111I	GACNNNGTC	1 cut(s)	1439
Van91I	CCANNNNNTGG	1 cut(s)	1649
VpaK11BI	GGWCC	2 cut(s)	31, 1304
XapI	RAATTY	2 cut(s)	415, 1699
XceI	RCATGY	2 cut(s)	256, 1909
XmiI	GTMKAC	1 cut(s)	1026
XspI	CTAG	7 cut(s)	329, 471, 659, 1299, 1524, 1856, 1931
ZraI	GACGTC	1 cut(s)	1918
Zsp2I	ATGCAT	1 cut(s)	1911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.