Rh7BG277900

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005759
Physical Location & Seq
Reverse (-)
25817564 .. 25825388
7825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG277900.1

Sequence Viewer

Length: 999 bp
ATGCAATCGCTAAATGCGGATGGTGTTGTTGTGGATTGTTGGTGGGGTATTGTTGAATGCTGGAGCCCCCAGAAATATGATTGGTCCGGTTATAGGGGTCTATTTAACATTGTACGCGAGTTCAAGCTAAAATTACAGGTTGTAATGGCATTTCATGAGTATGGAAGAAGCGAGTCCGGTGAAGCCCTTATTCCTCTTCCGAAGTGGATTTTAGACATTGGTAAAGAAAACCAGGACATATTCTTTACAGATCGAGAAGGAAGGAGGAATACTGAATGTCTATCCTGGGGCGTCGACAAAGAGAGAGTTTTGCATGGAAGAACTGGTGTTGAGGTCTATTTTGATTTCATGAGAAGTTTCCGTACCGAGTTTGATGACTTGTTTGGTGAGGGTCTTATTTCTGCTGTGGAGATTGGACTTGGAGCTTCAGGGGAGCTAAAGTATCCTTCCTTTTCAGAAAGGATGGGATGGAGATATCCTGGTATCGGCGAGTTTCAGTGTTATGATAGATACCTACAACAAAGTCTGCATAAAGCAGCAAAATTGCGTGGGCACTCATTCTGGGCTAGAGGACCAGATAATGCTGGTGAATACAATTCTCGGCCACATGAAACTGGCTTCTTCTGTGAGCGTGGTGATTATGATAGTTACTATGGACGCTTTTTCCTTCATTGGTATGCCAAGACACTAATTGACCATGCAGATAATGTCCTATCTCTTGCAAGCCTTGCCTTTGATGAAACAAAAGTAATTGTCAAGGTTCCTGCCGTTTATTGGTGGTACAAAACACCTAGCCATGCGGCAGAGCTAACTTCTGGGTACTATAATCCTAGAAACCAAGATGGGTACTCTCCAGTGTTTGAAGTTCTGAAGAAACATTCAGTGACGGTTAAGTTTGTTTGCTCGGCACCAAATGATGGTGATGAAGCATTGGGTGATCCAGAAGGTTTGAGTTGGCAGGTGATGGACCTTGTTCCTTGGCTCTGTAGCCGTTTATAA

Protein Analysis

332

Amino Acids

38.27

Weight (kDa)

5.45

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 7 - 321 8.1e-89 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 997
AarI CACCTGC 1 cut(s) 949
Acc36I ACCTGC 1 cut(s) 949
AccB1I GGYRCC 1 cut(s) 907
AccB7I CCANNNNNTGG 1 cut(s) 917
AccI GTMKAC 1 cut(s) 294
AccII CGCG 1 cut(s) 117
AciI CCGC 2 cut(s) 17, 800
AclWI GGATC 1 cut(s) 932
AcoI YGGCCR 1 cut(s) 602
AcuI CTGAAG 2 cut(s) 411, 890
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 5 cut(s) 114, 364, 782, 821, 848
AfiI CCNNNNNNNGG 4 cut(s) 93, 485, 774, 917
AgsI TTSAA 3 cut(s) 56, 124, 863
AjnI CCWGG 3 cut(s) 231, 284, 478
AluBI AGCT 4 cut(s) 127, 425, 436, 808
AluI AGCT 4 cut(s) 127, 425, 436, 808
AlwI GGATC 1 cut(s) 932
AoxI GGCC 1 cut(s) 602
ApeKI GCWGC 1 cut(s) 536
AspS9I GGNCC 3 cut(s) 84, 572, 967
AsuHPI GGTGA 7 cut(s) 191, 398, 599, 647, 932, 947, 973
AvaII GGWCC 3 cut(s) 84, 572, 967
BaeGI GKGCMC 1 cut(s) 555
BanI GGYRCC 1 cut(s) 907
BanII GRGCYC 1 cut(s) 68
BarI GAAGNNNNNNTAC 4 cut(s) 253, 285, 346, 378
BbvI GCAGC 1 cut(s) 548
BccI CCATC 6 cut(s) 14, 457, 462, 836, 911, 958
BceAI ACGGC 2 cut(s) 752, 975
BciT130I CCWGG 3 cut(s) 233, 286, 480
BciVI GTATCC 1 cut(s) 453
BfaI CTAG 3 cut(s) 567, 792, 831
BfmI CTRYAG 1 cut(s) 985
BfuAI ACCTGC 1 cut(s) 949
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 2 cut(s) 537, 801
BlsI GCNGC 2 cut(s) 538, 802
Bme1390I CCNGG 3 cut(s) 233, 286, 480
Bme18I GGWCC 3 cut(s) 84, 572, 967
BmgT120I GGNCC 3 cut(s) 84, 572, 967
BmiI GGNNCC 3 cut(s) 65, 762, 909
BmrFI CCNGG 3 cut(s) 233, 286, 480
BpmI CTGGAG 2 cut(s) 82, 837
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 285, 977
BsaWI WCCGGW 2 cut(s) 86, 176
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 485, 774, 917
Bse1I ACTGG 3 cut(s) 328, 619, 854
BseBI CCWGG 3 cut(s) 233, 286, 480
BseDI CCNNGG 2 cut(s) 285, 977
BseGI GGATG 3 cut(s) 25, 468, 473
BseLI CCNNNNNNNGG 4 cut(s) 93, 485, 774, 917
BseNI ACTGG 3 cut(s) 328, 619, 854
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 1 cut(s) 548
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 604
BshNI GGYRCC 1 cut(s) 907
BsiSI CCGG 2 cut(s) 87, 177
BslI CCNNNNNNNGG 4 cut(s) 93, 485, 774, 917
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 1 cut(s) 604
Bsp1286I GDGCHC 2 cut(s) 68, 555
Bsp143I GATC 2 cut(s) 250, 937
BspACI CCGC 2 cut(s) 17, 800
BspANI GGCC 1 cut(s) 604
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 2 cut(s) 154, 348
BspLI GGNNCC 3 cut(s) 65, 762, 909
BspMI ACCTGC 1 cut(s) 949
BspPI GGATC 1 cut(s) 932
BspT107I GGYRCC 1 cut(s) 907
BsrI ACTGG 3 cut(s) 328, 619, 854
BssECI CCNNGG 2 cut(s) 285, 977
BssMI GATC 2 cut(s) 250, 937
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 977
Bst2UI CCWGG 3 cut(s) 233, 286, 480
Bst4CI ACNGT 1 cut(s) 889
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 291
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 1 cut(s) 724
BstF5I GGATG 3 cut(s) 25, 468, 473
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 2 cut(s) 253, 940
BstMBI GATC 2 cut(s) 250, 937
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNI CCWGG 3 cut(s) 233, 286, 480
BstSCI CCNGG 3 cut(s) 231, 284, 478
BstSFI CTRYAG 1 cut(s) 985
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 548
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 1 cut(s) 604
BtsCI GGATG 3 cut(s) 25, 468, 473
BtsIMutI CAGTG 3 cut(s) 503, 861, 888
BveI ACCTGC 1 cut(s) 949
Cac8I GCNNGC 1 cut(s) 724
CciI TCATGA 2 cut(s) 154, 348
Cfr13I GGNCC 3 cut(s) 84, 572, 967
CseI GACGC 2 cut(s) 280, 666
Csp6I GTAC 5 cut(s) 113, 363, 781, 820, 847
CviAII CATG 6 cut(s) 155, 314, 349, 608, 698, 797
CviQI GTAC 5 cut(s) 113, 363, 781, 820, 847
DpnI GATC 2 cut(s) 252, 939
DpnII GATC 2 cut(s) 250, 937
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 977
Eco24I GRGCYC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 476
Eco47I GGWCC 3 cut(s) 84, 572, 967
Eco57I CTGAAG 2 cut(s) 411, 890
EcoRII CCWGG 3 cut(s) 231, 284, 478
EcoRV GATATC 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 977
EcoT38I GRGCYC 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 977
FaeI CATG 6 cut(s) 158, 317, 352, 611, 701, 800
FatI CATG 6 cut(s) 154, 313, 348, 607, 697, 796
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 537, 801
FokI GGATG 3 cut(s) 32, 475, 480
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 537, 801
FspBI CTAG 3 cut(s) 567, 792, 831
GluI GCNGC 2 cut(s) 537, 801
GsuI CTGGAG 2 cut(s) 82, 837
HaeIII GGCC 1 cut(s) 604
HapII CCGG 2 cut(s) 87, 177
HgaI GACGC 2 cut(s) 280, 666
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 6 cut(s) 158, 317, 352, 611, 701, 800
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 173
HpaII CCGG 2 cut(s) 87, 177
HphI GGTGA 7 cut(s) 191, 398, 599, 647, 932, 947, 973
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 3 cut(s) 201, 457, 870
Hpy188III TCNNGA 4 cut(s) 155, 254, 349, 941
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 5 cut(s) 251, 255, 456, 677, 938
HpyCH4III ACNGT 1 cut(s) 889
HpyCH4V TGCA 5 cut(s) 4, 313, 529, 701, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 6 cut(s) 158, 317, 352, 611, 701, 800
Kzo9I GATC 2 cut(s) 250, 937
LmnI GCTCC 3 cut(s) 63, 422, 433
Lsp1109I GCAGC 1 cut(s) 548
MaeI CTAG 3 cut(s) 567, 792, 831
MaeIII GTNAC 2 cut(s) 647, 883
MalI GATC 2 cut(s) 252, 939
MboI GATC 2 cut(s) 250, 937
MboII GAAGA 5 cut(s) 177, 188, 330, 613, 883
MhlI GDGCHC 2 cut(s) 68, 555
MluCI AATT 5 cut(s) 131, 542, 595, 690, 750
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 5 cut(s) 204, 258, 325, 382, 563
MseI TTAA 2 cut(s) 105, 891
MslI CAYNNNNRTG 2 cut(s) 159, 675
MspI CCGG 2 cut(s) 87, 177
MspR9I CCNGG 3 cut(s) 233, 286, 480
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 3 cut(s) 233, 286, 480
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 728
NdeII GATC 2 cut(s) 250, 937
NlaIII CATG 6 cut(s) 158, 317, 352, 611, 701, 800
NlaIV GGNNCC 3 cut(s) 65, 762, 909
NmeAIII GCCGAG 2 cut(s) 580, 884
NmuCI GTSAC 1 cut(s) 883
PagI TCATGA 2 cut(s) 154, 348
PaqCI CACCTGC 1 cut(s) 949
PctI GAATGC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 917
PkrI GCNGC 2 cut(s) 538, 802
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PsiI TTATAA 1 cut(s) 997
Psp6I CCWGG 3 cut(s) 231, 284, 478
PspGI CCWGG 3 cut(s) 231, 284, 478
PspN4I GGNNCC 3 cut(s) 65, 762, 909
PspPI GGNCC 3 cut(s) 84, 572, 967
RsaI GTAC 5 cut(s) 114, 364, 782, 821, 848
RsaNI GTAC 5 cut(s) 113, 363, 781, 820, 847
RseI CAYNNNNRTG 2 cut(s) 159, 675
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 105, 891
SatI GCNGC 2 cut(s) 537, 801
Sau3AI GATC 2 cut(s) 250, 937
Sau96I GGNCC 3 cut(s) 84, 572, 967
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 233, 286, 480
SduI GDGCHC 2 cut(s) 68, 555
SfcI CTRYAG 1 cut(s) 985
SinI GGWCC 3 cut(s) 84, 572, 967
SmiMI CAYNNNNRTG 2 cut(s) 159, 675
Sse9I AATT 5 cut(s) 131, 542, 595, 690, 750
SsiI CCGC 2 cut(s) 17, 800
SspMI CTAG 3 cut(s) 567, 792, 831
StyD4I CCNGG 3 cut(s) 231, 284, 478
StyI CCWWGG 1 cut(s) 977
TaaI ACNGT 1 cut(s) 889
TaqI TCGA 2 cut(s) 253, 294
TasI AATT 5 cut(s) 131, 542, 595, 690, 750
TauI GCSGC 1 cut(s) 803
Tru1I TTAA 2 cut(s) 105, 891
Tru9I TTAA 2 cut(s) 105, 891
TscAI CASTG 3 cut(s) 503, 861, 888
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 6 cut(s) 143, 337, 624, 659, 753, 939
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 3 cut(s) 503, 861, 888
Van91I CCANNNNNTGG 1 cut(s) 917
VpaK11BI GGWCC 3 cut(s) 84, 572, 967
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 3 cut(s) 567, 792, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.