Rh7CG305300

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0005759
Physical Location & Seq
Reverse (-)
31978695 .. 31984846
6152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG305300.1

Sequence Viewer

Length: 1212 bp
ATGCAATCGCTAAATGCGGATGGTGTTGTTGTGGATTGTTGGTGGGGTATTGTTGAATGCTGGAGCCCCCAGAAATACGATTGGTCCGGTTATAGGGGTCTATTTAACATTGTACGCGAGTTCAAGCTAAAATTACAGGTTGTAATGGCATTTCATGAGTATGGAAGAAGTGAGTCCGGTGAAGCCCTTATTCCTCTTCCGAAGTGGATTTTAGACATTGGTAAAGAAAACCAGGACATATTCTTTACAGATCGAGAAGGAAGGAGGAATACTGAATGTCTATCCTGGGGCGTCGACAAAGAGAGGGTTTTGCATGGAAGAACTGGTGTTGAGGTCTATTTTGATTTCATGAGAAGTTTCCGTACCGAGTTTGATGACTTGTTTGGTGAGGGTCTTATTTCTGCTGTGGAGATTGGACTTGGAGCTTCTGGGGAGCTAAAGTATCCTTCCTTTTCAGAAAGGATGGGATGGAGATATCCTGGTATCGGCGAGTTTCAGTGTTATGATAGATACCTACAACAAAGTCTGCATAAAGCAGCAAAATTGCGTGGGCACTCATTCTGGGCTAGAGGACCAGATAATGCTGGTGAATACAATTCTCGGCCACATGAAACTGGCTTCTTCTGTGAGCGTGGTGATTATGATAGTTACTATGGACGCTTTTTCCTTCATTGGTATGCCAAGACACTAATTGACCATGCAGATAATGTCCTATCTCTTGCAAGCCTTGCCTTTGATGAAACAAAAGTAATTGTCAAGGTTCCTGCTGTTTATTGGTGGTACAAAACACCTAGCCATGCAGCAGAGCTAACTTCTGGGTACTATAATCCTAGAAACCAAGATGGGTACTCTCCAGTGTTTGAAGTTCTGAAGAAACATTCAGTGACGGTTAAGTTTGTTTGCTCGGCACCAAATGATGGTGATGAAGCATTGGGTGATCCAGAAGGTTTGAGTTGGCAGGTTTTAAATTTAGCTTGGGATAGAGGATTGACTGTGGCTGGGGAAAATGCACTGTCATGCTATGATAGAGAAGGGTGCATGAGAATTGTTGAAATGGTGAAGCCAAGAAATGATCCTGATCGCCACCATTTCACATTCTTTGTATATCAGCAACCATCTCCTCTAGTTCAAGGGACAATTTGCTTTTCGGAGTTGGACTTTTTCATCAAGTGCATGCATGGTGACATGGCTGGGAATATAGTGTCGTGTTAG

Protein Analysis

403

Amino Acids

46.29

Weight (kDa)

5.44

Isoelectric Point (pI)

34.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 7 - 360 3.2e-96 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 949
AccB1I GGYRCC 1 cut(s) 907
AccB7I CCANNNNNTGG 1 cut(s) 917
AccI GTMKAC 1 cut(s) 294
AccII CGCG 1 cut(s) 117
AciI CCGC 1 cut(s) 17
AclWI GGATC 2 cut(s) 932, 1067
AcoI YGGCCR 1 cut(s) 602
AcsI RAATTY 1 cut(s) 967
AcuI CTGAAG 1 cut(s) 890
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 5 cut(s) 114, 364, 782, 821, 848
AfiI CCNNNNNNNGG 3 cut(s) 93, 485, 917
AgsI TTSAA 5 cut(s) 56, 124, 863, 1052, 1130
AjnI CCWGG 3 cut(s) 231, 284, 478
AluBI AGCT 5 cut(s) 127, 425, 436, 808, 974
AluI AGCT 5 cut(s) 127, 425, 436, 808, 974
AlwI GGATC 2 cut(s) 932, 1067
AoxI GGCC 1 cut(s) 602
ApeKI GCWGC 2 cut(s) 536, 800
ApoI RAATTY 1 cut(s) 967
AspS9I GGNCC 2 cut(s) 84, 572
AsuHPI GGTGA 8 cut(s) 191, 398, 599, 647, 932, 947, 1069, 1193
AvaII GGWCC 2 cut(s) 84, 572
BaeGI GKGCMC 1 cut(s) 555
BanI GGYRCC 1 cut(s) 907
BanII GRGCYC 1 cut(s) 68
BarI GAAGNNNNNNTAC 4 cut(s) 253, 285, 346, 378
BbvI GCAGC 2 cut(s) 548, 812
BccI CCATC 6 cut(s) 14, 457, 462, 836, 911, 1123
BciT130I CCWGG 3 cut(s) 233, 286, 480
BciVI GTATCC 1 cut(s) 453
BfaI CTAG 4 cut(s) 567, 792, 831, 1124
BfuAI ACCTGC 1 cut(s) 949
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 2 cut(s) 537, 801
BlsI GCNGC 2 cut(s) 538, 802
Bme1390I CCNGG 3 cut(s) 233, 286, 480
Bme18I GGWCC 2 cut(s) 84, 572
BmgT120I GGNCC 2 cut(s) 84, 572
BmiI GGNNCC 3 cut(s) 65, 762, 909
BmrFI CCNGG 3 cut(s) 233, 286, 480
BpmI CTGGAG 2 cut(s) 82, 837
BsaBI GATNNNNATC 1 cut(s) 1077
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 285
BsaWI WCCGGW 2 cut(s) 86, 176
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 485, 917
Bse1I ACTGG 3 cut(s) 328, 619, 854
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 3 cut(s) 233, 286, 480
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 3 cut(s) 25, 468, 473
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 3 cut(s) 93, 485, 917
BseNI ACTGG 3 cut(s) 328, 619, 854
BseRI GAGGAG 1 cut(s) 1110
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 2 cut(s) 548, 812
BseYI CCCAGC 2 cut(s) 998, 1190
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 604
BshNI GGYRCC 1 cut(s) 907
BsiSI CCGG 2 cut(s) 87, 177
BslFI GGGAC 1 cut(s) 1147
BslI CCNNNNNNNGG 3 cut(s) 93, 485, 917
BsmFI GGGAC 1 cut(s) 1147
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 1 cut(s) 604
Bsp1286I GDGCHC 2 cut(s) 68, 555
Bsp143I GATC 4 cut(s) 250, 937, 1072, 1078
BspACI CCGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 604
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 2 cut(s) 154, 348
BspLI GGNNCC 3 cut(s) 65, 762, 909
BspMI ACCTGC 1 cut(s) 949
BspPI GGATC 2 cut(s) 932, 1067
BspT107I GGYRCC 1 cut(s) 907
BsrI ACTGG 3 cut(s) 328, 619, 854
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 4 cut(s) 250, 937, 1072, 1078
BssNI GRCGYC 1 cut(s) 291
Bst2UI CCWGG 3 cut(s) 233, 286, 480
Bst4CI ACNGT 3 cut(s) 889, 994, 1014
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 291
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 724, 1175
BstF5I GGATG 3 cut(s) 25, 468, 473
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 4 cut(s) 253, 940, 1075, 1081
BstMBI GATC 4 cut(s) 250, 937, 1072, 1078
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNI CCWGG 3 cut(s) 233, 286, 480
BstNSI RCATGY 1 cut(s) 1177
BstSCI CCNGG 3 cut(s) 231, 284, 478
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 2 cut(s) 548, 812
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 1 cut(s) 604
BtsCI GGATG 3 cut(s) 25, 468, 473
BtsIMutI CAGTG 4 cut(s) 503, 861, 888, 1010
BveI ACCTGC 1 cut(s) 949
Cac8I GCNNGC 2 cut(s) 724, 1175
CciI TCATGA 2 cut(s) 154, 348
Cfr13I GGNCC 2 cut(s) 84, 572
CseI GACGC 2 cut(s) 280, 666
Csp6I GTAC 5 cut(s) 113, 363, 781, 820, 847
CviQI GTAC 5 cut(s) 113, 363, 781, 820, 847
DpnI GATC 4 cut(s) 252, 939, 1074, 1080
DpnII GATC 4 cut(s) 250, 937, 1072, 1078
DraI TTTAAA 1 cut(s) 966
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 84, 572
Eco57I CTGAAG 1 cut(s) 890
EcoRII CCWGG 3 cut(s) 231, 284, 478
EcoRV GATATC 1 cut(s) 476
EcoT22I ATGCAT 1 cut(s) 1179
EcoT38I GRGCYC 1 cut(s) 68
FaqI GGGAC 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 537, 801
FokI GGATG 3 cut(s) 32, 475, 480
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 537, 801
FspBI CTAG 4 cut(s) 567, 792, 831, 1124
GluI GCNGC 2 cut(s) 537, 801
GsaI CCCAGC 2 cut(s) 1002, 1194
GsuI CTGGAG 2 cut(s) 82, 837
HaeIII GGCC 1 cut(s) 604
HapII CCGG 2 cut(s) 87, 177
HgaI GACGC 2 cut(s) 280, 666
Hin1I GRCGYC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 173
HpaII CCGG 2 cut(s) 87, 177
HphI GGTGA 8 cut(s) 191, 398, 599, 647, 932, 947, 1069, 1193
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 4 cut(s) 201, 457, 870, 1150
Hpy188III TCNNGA 5 cut(s) 155, 254, 349, 941, 1076
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 6 cut(s) 251, 255, 456, 677, 938, 1025
HpyCH4III ACNGT 3 cut(s) 889, 994, 1014
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
Hsp92I GRCGYC 1 cut(s) 291
Kzo9I GATC 4 cut(s) 250, 937, 1072, 1078
LmnI GCTCC 3 cut(s) 63, 422, 433
Lsp1109I GCAGC 2 cut(s) 548, 812
MaeI CTAG 4 cut(s) 567, 792, 831, 1124
MaeIII GTNAC 3 cut(s) 647, 883, 1181
MalI GATC 4 cut(s) 252, 939, 1074, 1080
MboI GATC 4 cut(s) 250, 937, 1072, 1078
MboII GAAGA 5 cut(s) 177, 188, 330, 613, 883
MhlI GDGCHC 2 cut(s) 68, 555
MluCI AATT 8 cut(s) 131, 542, 595, 690, 750, 967, 1044, 1137
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 1134
MnlI CCTC 8 cut(s) 204, 258, 297, 325, 382, 563, 977, 1131
Mph1103I ATGCAT 1 cut(s) 1179
MseI TTAA 3 cut(s) 105, 891, 965
MslI CAYNNNNRTG 3 cut(s) 159, 675, 1015
MspI CCGG 2 cut(s) 87, 177
MspR9I CCNGG 3 cut(s) 233, 286, 480
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 3 cut(s) 233, 286, 480
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 728
NdeII GATC 4 cut(s) 250, 937, 1072, 1078
NlaIV GGNNCC 3 cut(s) 65, 762, 909
NmeAIII GCCGAG 2 cut(s) 580, 884
NmuCI GTSAC 2 cut(s) 883, 1181
NsiI ATGCAT 1 cut(s) 1179
NspI RCATGY 1 cut(s) 1177
PaeI GCATGC 1 cut(s) 1177
PagI TCATGA 2 cut(s) 154, 348
PctI GAATGC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 917
PkrI GCNGC 2 cut(s) 538, 802
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp6I CCWGG 3 cut(s) 231, 284, 478
PspFI CCCAGC 2 cut(s) 998, 1190
PspGI CCWGG 3 cut(s) 231, 284, 478
PspN4I GGNNCC 3 cut(s) 65, 762, 909
PspPI GGNCC 2 cut(s) 84, 572
RsaI GTAC 5 cut(s) 114, 364, 782, 821, 848
RsaNI GTAC 5 cut(s) 113, 363, 781, 820, 847
RseI CAYNNNNRTG 3 cut(s) 159, 675, 1015
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 105, 891, 965
SatI GCNGC 2 cut(s) 537, 801
Sau3AI GATC 4 cut(s) 250, 937, 1072, 1078
Sau96I GGNCC 2 cut(s) 84, 572
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 233, 286, 480
SduI GDGCHC 2 cut(s) 68, 555
SinI GGWCC 2 cut(s) 84, 572
SmiMI CAYNNNNRTG 3 cut(s) 159, 675, 1015
SphI GCATGC 1 cut(s) 1177
Sse9I AATT 8 cut(s) 131, 542, 595, 690, 750, 967, 1044, 1137
SsiI CCGC 1 cut(s) 17
SspMI CTAG 4 cut(s) 567, 792, 831, 1124
StyD4I CCNGG 3 cut(s) 231, 284, 478
TaaI ACNGT 3 cut(s) 889, 994, 1014
TaqI TCGA 2 cut(s) 253, 294
TasI AATT 8 cut(s) 131, 542, 595, 690, 750, 967, 1044, 1137
Tru1I TTAA 3 cut(s) 105, 891, 965
Tru9I TTAA 3 cut(s) 105, 891, 965
TscAI CASTG 4 cut(s) 503, 861, 888, 1017
TseFI GTSAC 2 cut(s) 883, 1181
TseI GCWGC 2 cut(s) 536, 800
Tsp45I GTSAC 2 cut(s) 883, 1181
TspDTI ATGAA 7 cut(s) 143, 337, 624, 659, 753, 939, 1153
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 4 cut(s) 503, 861, 888, 1017
Van91I CCANNNNNTGG 1 cut(s) 917
VpaK11BI GGWCC 2 cut(s) 84, 572
XapI RAATTY 1 cut(s) 967
XceI RCATGY 1 cut(s) 1177
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 4 cut(s) 567, 792, 831, 1124
Zsp2I ATGCAT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.