Rh7CG305400

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0005759
Physical Location & Seq
Reverse (-)
31985437 .. 31985841
405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG305400.1

Sequence Viewer

Length: 405 bp
ATGAACGACGAGAGCCTCCGGCAGGATTTGGACCCCCAAAGCGATCAGAGCTCCGATTACCTCCCACCACCTCAGCCCCACCACCCTCTCCCTTCCCAACCCCAACCCCCTCGCCGCCCACGTGGCTTCGCCGCCACTGCCGCAGCCGGCTCGGTGGGCCCCGTCTCTCCCTCCTCCACCAAGGGGGGAAGGAGAGAGAGGGAGAAGGAGAAGGAGCGGACGAAGCTCCGAGAACGCCTCCGCCGGTCAATCACCAGCCGTATGCTCGCCGGACTCCGCCAGTACGGCAATTTCCCCCTGCCTGCGCGCGCAGACATGAACGATGTGCTCGCAGCTCTGGCGCGTGAGGCCGGCTGGGTCGTCGAGACTGACGGCACCACTTATCGCCAGTCTCCACCTCAAATT

Protein Analysis

135

Amino Acids

15.01

Weight (kDa)

10.08

Isoelectric Point (pI)

81.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BES1_N PF05687 62 - 132 4.6e-40 BES1/BZR1 plant transcription factor, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 374
AccBSI CCGCTC 1 cut(s) 217
AccII CGCG 3 cut(s) 307, 309, 343
AciI CCGC 6 cut(s) 115, 132, 141, 217, 241, 277
AcvI CACGTG 1 cut(s) 122
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 2 cut(s) 22, 183
AluBI AGCT 3 cut(s) 51, 226, 335
AluI AGCT 3 cut(s) 51, 226, 335
Alw21I GWGCWC 2 cut(s) 53, 330
Alw26I GTCTC 3 cut(s) 169, 359, 396
AoxI GGCC 2 cut(s) 157, 348
ApaI GGGCCC 1 cut(s) 161
ApeKI GCWGC 2 cut(s) 143, 332
AspLEI GCGC 4 cut(s) 307, 309, 311, 343
AspS9I GGNCC 3 cut(s) 31, 157, 158
AsuHPI GGTGA 1 cut(s) 244
AvaII GGWCC 1 cut(s) 31
BaeGI GKGCMC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 374
BanII GRGCYC 2 cut(s) 53, 161
BbrPI CACGTG 1 cut(s) 122
Bbv12I GWGCWC 2 cut(s) 53, 330
BbvCI CCTCAGC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 155, 344
BceAI ACGGC 3 cut(s) 243, 301, 388
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcoDI GTCTC 3 cut(s) 169, 359, 396
BglI GCCNNNNNGGC 2 cut(s) 123, 285
BisI GCNGC 5 cut(s) 115, 132, 141, 144, 333
BlsI GCNGC 5 cut(s) 116, 133, 142, 145, 334
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 3 cut(s) 31, 157, 158
BmiI GGNNCC 4 cut(s) 33, 159, 160, 376
BplI GAGNNNNNCTC 2 cut(s) 222, 254
Bpu10I CCTNAGC 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 180
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 183
Bse118I RCCGGY 3 cut(s) 146, 243, 350
Bse1I ACTGG 2 cut(s) 280, 388
BseDI CCNNGG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 22, 183
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 2 cut(s) 280, 388
BsePI GCGCGC 2 cut(s) 305, 307
BseRI GAGGAG 1 cut(s) 163
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 2 cut(s) 155, 344
BseYI CCCAGC 1 cut(s) 354
Bsh1236I CGCG 3 cut(s) 307, 309, 343
BshFI GGCC 2 cut(s) 159, 350
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 2 cut(s) 53, 330
BsiSI CCGG 5 cut(s) 19, 147, 244, 270, 351
BslI CCNNNNNNNGG 2 cut(s) 22, 183
BsmAI GTCTC 3 cut(s) 169, 359, 396
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 2 cut(s) 159, 350
Bsp120I GGGCCC 1 cut(s) 157
Bsp1286I GDGCHC 3 cut(s) 53, 161, 330
Bsp143I GATC 1 cut(s) 43
BspACI CCGC 6 cut(s) 115, 132, 141, 217, 241, 277
BspANI GGCC 2 cut(s) 159, 350
BspCNI CTCAG 1 cut(s) 85
BspFNI CGCG 3 cut(s) 307, 309, 343
BspLI GGNNCC 4 cut(s) 33, 159, 160, 376
BspT107I GGYRCC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 217
BsrFI RCCGGY 3 cut(s) 146, 243, 350
BsrI ACTGG 2 cut(s) 280, 388
BssAI RCCGGY 3 cut(s) 146, 243, 350
BssECI CCNNGG 1 cut(s) 180
BssHII GCGCGC 2 cut(s) 305, 307
BssMI GATC 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 122
BstC8I GCNNGC 7 cut(s) 148, 267, 303, 307, 309, 330, 352
BstDEI CTNAG 1 cut(s) 72
BstENI CCTNNNNNAGG 1 cut(s) 20
BstFNI CGCG 3 cut(s) 307, 309, 343
BstHHI GCGC 4 cut(s) 307, 309, 311, 343
BstKTI GATC 1 cut(s) 46
BstMAI GTCTC 3 cut(s) 169, 359, 396
BstMBI GATC 1 cut(s) 43
BstMWI GCNNNNNNNGC 9 cut(s) 48, 123, 137, 140, 156, 223, 285, 338, 347
BstSLI GKGCMC 1 cut(s) 161
BstUI CGCG 3 cut(s) 307, 309, 343
BstV1I GCAGC 2 cut(s) 155, 344
BsuRI GGCC 2 cut(s) 159, 350
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 7 cut(s) 148, 267, 303, 307, 309, 330, 352
CfoI GCGC 4 cut(s) 307, 309, 311, 343
Cfr10I RCCGGY 3 cut(s) 146, 243, 350
Cfr13I GGNCC 3 cut(s) 31, 157, 158
Csp6I GTAC 1 cut(s) 283
CviAII CATG 1 cut(s) 316
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 72
DpnI GATC 1 cut(s) 45
DpnII GATC 1 cut(s) 43
EciI GGCGGA 2 cut(s) 230, 266
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 2 cut(s) 53, 161
Eco47I GGWCC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 51
Eco72I CACGTG 1 cut(s) 122
EcoICRI GAGCTC 1 cut(s) 51
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 180
EcoT38I GRGCYC 2 cut(s) 53, 161
ErhI CCWWGG 1 cut(s) 180
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 1 cut(s) 319
FaiI YATR 2 cut(s) 263, 317
FatI CATG 1 cut(s) 315
Fnu4HI GCNGC 5 cut(s) 115, 132, 141, 144, 333
FriOI GRGCYC 2 cut(s) 53, 161
Fsp4HI GCNGC 5 cut(s) 115, 132, 141, 144, 333
GlaI GCGC 4 cut(s) 306, 308, 310, 342
GluI GCNGC 5 cut(s) 115, 132, 141, 144, 333
GsaI CCCAGC 1 cut(s) 358
HaeIII GGCC 2 cut(s) 159, 350
HapII CCGG 5 cut(s) 19, 147, 244, 270, 351
HhaI GCGC 4 cut(s) 307, 309, 311, 343
Hin1II CATG 1 cut(s) 319
Hin6I GCGC 4 cut(s) 305, 307, 309, 341
HinP1I GCGC 4 cut(s) 305, 307, 309, 341
HinfI GANTC 1 cut(s) 273
HpaII CCGG 5 cut(s) 19, 147, 244, 270, 351
HphI GGTGA 1 cut(s) 244
Hpy188I TCNGA 3 cut(s) 48, 55, 230
Hpy188III TCNNGA 1 cut(s) 364
Hpy99I CGWCG 2 cut(s) 11, 365
HpyAV CCTTC 4 cut(s) 102, 183, 199, 205
HpyCH4IV ACGT 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 9 cut(s) 48, 123, 137, 140, 156, 223, 285, 338, 347
HpyF3I CTNAG 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 121
Hsp92II CATG 1 cut(s) 319
HspAI GCGC 4 cut(s) 305, 307, 309, 341
KroI GCCGGC 2 cut(s) 146, 350
KroNI GCCGGC 2 cut(s) 148, 352
Kzo9I GATC 1 cut(s) 43
LmnI GCTCC 3 cut(s) 56, 214, 231
Lsp1109I GCAGC 2 cut(s) 155, 344
MaeII ACGT 1 cut(s) 121
MalI GATC 1 cut(s) 45
MbiI CCGCTC 1 cut(s) 217
MboI GATC 1 cut(s) 43
MhlI GDGCHC 3 cut(s) 53, 161, 330
MluCI AATT 1 cut(s) 289
MlyI GAGTC 1 cut(s) 267
MroNI GCCGGC 2 cut(s) 146, 350
MspI CCGG 5 cut(s) 19, 147, 244, 270, 351
MvnI CGCG 3 cut(s) 307, 309, 343
MwoI GCNNNNNNNGC 9 cut(s) 48, 123, 137, 140, 156, 223, 285, 338, 347
NaeI GCCGGC 2 cut(s) 148, 352
NdeII GATC 1 cut(s) 43
NgoMIV GCCGGC 2 cut(s) 146, 350
NlaIII CATG 1 cut(s) 319
NlaIV GGNNCC 4 cut(s) 33, 159, 160, 376
PauI GCGCGC 2 cut(s) 305, 307
PcsI WCGNNNNNNNCGW 1 cut(s) 118
PdiI GCCGGC 2 cut(s) 148, 352
PkrI GCNGC 5 cut(s) 116, 133, 142, 145, 334
PleI GAGTC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 122
PmlI CACGTG 1 cut(s) 122
PpsI GAGTC 1 cut(s) 267
Ppu21I YACGTR 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 53
PspCI CACGTG 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 354
PspN4I GGNNCC 4 cut(s) 33, 159, 160, 376
PspOMI GGGCCC 1 cut(s) 157
PspPI GGNCC 3 cut(s) 31, 157, 158
PteI GCGCGC 2 cut(s) 305, 307
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SacI GAGCTC 1 cut(s) 53
SatI GCNGC 5 cut(s) 115, 132, 141, 144, 333
Sau3AI GATC 1 cut(s) 43
Sau96I GGNCC 3 cut(s) 31, 157, 158
SchI GAGTC 1 cut(s) 267
SduI GDGCHC 3 cut(s) 53, 161, 330
SetI ASST 7 cut(s) 53, 63, 73, 124, 228, 337, 400
SinI GGWCC 1 cut(s) 31
Sse9I AATT 1 cut(s) 289
SsiI CCGC 6 cut(s) 115, 132, 141, 217, 241, 277
SstI GAGCTC 1 cut(s) 53
StyI CCWWGG 1 cut(s) 180
TaiI ACGT 1 cut(s) 124
TaqI TCGA 1 cut(s) 363
TasI AATT 1 cut(s) 289
TauI GCSGC 3 cut(s) 117, 134, 143
TscAI CASTG 1 cut(s) 142
TseI GCWGC 2 cut(s) 143, 332
TspDTI ATGAA 2 cut(s) 17, 332
TspRI CASTG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 31
XagI CCTNNNNNAGG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.