FvH4_5g25231

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016859
Physical Location & Seq
Reverse (-)
16696859 .. 16697505
647 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25231.t1

Sequence Viewer

Length: 378 bp
ATGTCGGGAATGCGTCTCTCCTCGAACAAACTTCGAATCATCTTCTACCAGCTCGTCATGCAGCTCGTCATGGGACTTCGTGTTGCTCACTCTGCACGAATTTCTCTCAGGGACCTCCGCCCGGAGAAAATTAGGGTTTTGAGGAGCTCTTCCATGCTAAGCTCTCTGATTTTGCTAGGGCTAAGATTCTTCACCCATATGCTTAACATGGTGACGCCGCCAGGAGCACTCGAGCGTTGCCTGAGCTACCAAGCCGAAGAGCAACTCTTCGGTGAGAGTAAAAATGAGGGTGAGACCAAAGGTGGTCTCTTCACTCTCTTGCATATGCTTCATTGTTGCATACTAGATCGATTCATTTTCATTGGTAGTTTGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.32

Weight (kDa)

9.81

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 118, 218
AcsI RAATTY 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 121
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 5 cut(s) 52, 64, 147, 162, 246
AluI AGCT 5 cut(s) 52, 64, 147, 162, 246
Alw21I GWGCWC 2 cut(s) 149, 229
Alw26I GTCTC 3 cut(s) 20, 287, 311
Ama87I CYCGRG 1 cut(s) 230
ApeKI GCWGC 1 cut(s) 61
ApoI RAATTY 1 cut(s) 99
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 4 cut(s) 184, 223, 284, 302
AsuII TTCGAA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 149
Bbv12I GWGCWC 2 cut(s) 149, 229
BbvI GCAGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 222
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 3 cut(s) 20, 287, 311
BfaI CTAG 2 cut(s) 176, 344
BisI GCNGC 2 cut(s) 62, 218
BlpI GCTNAGC 1 cut(s) 158
BlsI GCNGC 2 cut(s) 63, 219
Bme1390I CCNGG 2 cut(s) 122, 222
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 2 cut(s) 122, 222
Bpu10I CCTNAGC 1 cut(s) 242
Bpu1102I GCTNAGC 1 cut(s) 158
Bpu14I TTCGAA 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 215
BsaI GGTCTC 2 cut(s) 287, 311
Bsc4I CCNNNNNNNGG 1 cut(s) 121
BseBI CCWGG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 349
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 121, 233
BseRI GAGGAG 2 cut(s) 10, 157
BseXI GCAGC 1 cut(s) 73
BsgI GTGCAG 1 cut(s) 78
BshVI ATCGAT 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 149, 229
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 1 cut(s) 122
BslFI GGGAC 2 cut(s) 87, 125
BslI CCNNNNNNNGG 1 cut(s) 121
BsmAI GTCTC 3 cut(s) 20, 287, 311
BsmBI CGTCTC 1 cut(s) 20
BsmFI GGGAC 2 cut(s) 87, 125
BsmI GAATGC 1 cut(s) 15
Bso31I GGTCTC 2 cut(s) 287, 311
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 2 cut(s) 149, 229
Bsp143I GATC 1 cut(s) 346
Bsp1720I GCTNAGC 1 cut(s) 158
BspACI CCGC 2 cut(s) 118, 218
BspCNI CTCAG 2 cut(s) 120, 234
BspDI ATCGAT 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 113
BspQI GCTCTTC 2 cut(s) 154, 252
BspT104I TTCGAA 1 cut(s) 34
BspTNI GGTCTC 2 cut(s) 287, 311
BssMI GATC 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 222
Bst6I CTCTTC 4 cut(s) 154, 252, 272, 314
BstACI GRCGYC 1 cut(s) 215
BstBI TTCGAA 1 cut(s) 34
BstDEI CTNAG 4 cut(s) 107, 158, 182, 242
BstKTI GATC 1 cut(s) 349
BstMAI GTCTC 3 cut(s) 20, 287, 311
BstMBI GATC 1 cut(s) 346
BstMWI GCNNNNNNNGC 2 cut(s) 58, 92
BstNI CCWGG 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 120, 220
BstV1I GCAGC 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 349
BsuTUI ATCGAT 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 349
CseI GACGC 2 cut(s) 2, 223
CviAII CATG 4 cut(s) 58, 70, 154, 208
CviJI RGCY 7 cut(s) 52, 64, 147, 162, 181, 246, 254
CviKI_1 RGCY 7 cut(s) 52, 64, 147, 162, 181, 246, 254
DdeI CTNAG 4 cut(s) 107, 158, 182, 242
DpnI GATC 1 cut(s) 348
DpnII GATC 1 cut(s) 346
Eam1104I CTCTTC 4 cut(s) 154, 252, 272, 314
EarI CTCTTC 4 cut(s) 154, 252, 272, 314
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 2 cut(s) 287, 311
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 147
Eco88I CYCGRG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 4 cut(s) 61, 73, 157, 211
FaiI YATR 9 cut(s) 59, 71, 155, 198, 200, 209, 324, 326, 341
FaqI GGGAC 2 cut(s) 87, 125
FatI CATG 4 cut(s) 57, 69, 153, 207
FauNDI CATATG 2 cut(s) 198, 324
Fnu4HI GCNGC 2 cut(s) 62, 218
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 62, 218
FspBI CTAG 2 cut(s) 176, 344
GluI GCNGC 2 cut(s) 62, 218
HapII CCGG 1 cut(s) 122
HgaI GACGC 2 cut(s) 2, 223
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 4 cut(s) 61, 73, 157, 211
HinfI GANTC 3 cut(s) 36, 186, 351
HpaII CCGG 1 cut(s) 122
HphI GGTGA 4 cut(s) 184, 223, 284, 302
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 1 cut(s) 6
HpyCH4V TGCA 4 cut(s) 61, 95, 322, 339
HpyF10VI GCNNNNNNNGC 2 cut(s) 58, 92
HpyF3I CTNAG 4 cut(s) 107, 158, 182, 242
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 4 cut(s) 61, 73, 157, 211
Kzo9I GATC 1 cut(s) 346
LguI GCTCTTC 2 cut(s) 154, 252
LmnI GCTCC 2 cut(s) 144, 224
LpnPI CCDG 6 cut(s) 62, 94, 135, 207, 234, 254
Lsp1109I GCAGC 1 cut(s) 73
MaeI CTAG 2 cut(s) 176, 344
MaeIII GTNAC 1 cut(s) 211
MalI GATC 1 cut(s) 348
MboI GATC 1 cut(s) 346
MboII GAAGA 6 cut(s) 34, 141, 181, 259, 269, 301
MhlI GDGCHC 2 cut(s) 149, 229
MluCI AATT 2 cut(s) 99, 129
MnlI CCTC 4 cut(s) 31, 125, 135, 280
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 1 cut(s) 197
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 2 cut(s) 122, 222
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 58, 92
NciI CCSGG 1 cut(s) 122
NdeI CATATG 2 cut(s) 198, 324
NdeII GATC 1 cut(s) 346
NlaIII CATG 4 cut(s) 61, 73, 157, 211
NlaIV GGNNCC 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 211
NspV TTCGAA 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 230
PciSI GCTCTTC 2 cut(s) 154, 252
PctI GAATGC 1 cut(s) 15
PfeI GAWTC 3 cut(s) 36, 186, 351
PkrI GCNGC 2 cut(s) 63, 219
PpuMI RGGWCCY 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 1 cut(s) 112
PspPPI RGGWCCY 1 cut(s) 112
PspXI VCTCGAGB 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 149
SapI GCTCTTC 2 cut(s) 154, 252
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 2 cut(s) 62, 218
Sau3AI GATC 1 cut(s) 346
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 122, 222
SduI GDGCHC 2 cut(s) 149, 229
SetI ASST 7 cut(s) 54, 66, 117, 149, 164, 248, 304
Sfr274I CTCGAG 1 cut(s) 230
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 2 cut(s) 99, 129
SsiI CCGC 2 cut(s) 118, 218
SspMI CTAG 2 cut(s) 176, 344
SstI GAGCTC 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 120, 220
TaqI TCGA 4 cut(s) 23, 34, 231, 349
TasI AATT 2 cut(s) 99, 129
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 3 cut(s) 36, 186, 351
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 3 cut(s) 320, 343, 349
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 99
XhoI CTCGAG 1 cut(s) 230
XspI CTAG 2 cut(s) 176, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.