Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0004306

Physical Location & Seq

Genomic Coordinates Reverse (-)

17085946 .. 17097457

Gene Structure 11512 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g25570.t15

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3882 bp

ATGACCCTAACATTTTCCACACATGAGAACTCTCTTCACTCTTCCCTTTTACAGTTTCCTCAAGTTCCAAAAATGGCGTCTGCAATTCCGGGAACAAACCCTAGAACCTCGAAAACCCGGTACCGCATCGGAGACATCGATGTAGAGTTCCCCTACCAGCCTTACGGCGCCCAGATGGCCTTCATGAGCAGAGTCGTCTCCACTCTCAATCGAGCTGTCAGCGACGGCCACTGCCACGCCTTGCTCGAGTCTCCCACCGGAACCGGGAAATCGCTCTCGCTTCTCTGCTCGATTCTCGCTTGGCAGCAGAGCTATAAGGCCAAGAACATGTACACCAATCTATCACACTCCAAGCCCAACCCCAAGGCCATGACCGACCCCAAGGGACACGGCGGAGGCTTTGTTCCGGAAGAGGAGAGGTCAAGTGCTCAGCAATTCGAAAAGCCAGGGCCGTCTCAGTCTGGCGAAAATCGGAAGAAAAAGGCAGCTCCTACCATATACTATGCATCGAGGACACATTCACAAATTTCTCAAGTCATCCGTGAATATCGAAAAACTTCCTACAGGGTTACTATGGCAGTGTTGGCCTCTAGGAAGCATTACTGCACAAATGCGCAAGTACATGGGAGAGAGAATATCGATGAAGAATGTAAGCTACTTTTGAAGGATCGAGCCGTTGGATGCGTAGAGTTTAAAAATGTACACAAAGTCAAAGGACATCCATCTCTTCAGAAAGGAGGCTGCCATGAGGCCTACGATATTGAGGATCTCGTAAAAGTTGGAAAAGCTGTCAAAGGTTGTTCATATTATGCTGCCCGTTCTATGTCAGAAGATGCAGAATTGGTTTTTTGTCCATATAACTACATCATTAATCCAGTCATTAGGGGAGCCATGGAAGTAGATATCCAGGGGTCTATTGTAGTTCTGGATGAAGCCCACAATATAGAGGATGTAGCTCGTGATGCTGGTAGTGTGGATATTGATGAAGAAGTTTTGAACAAATTGCAGATGGAACTGGAGCAGCTTTGCCCGCTGGATGTTTTGGTTTACCAACCTCTGTATGAAATGATACAGGACCTCTTAAGTTGGATTGAGCGAAGGAAAGATAAGTTGGAAAAACGTGAATTTCAGCACTTTGTCTCTTGTTGGACCGGTGACAAGGCTTTGAGAGAACTCCACGAAGCTAATATATCACAGCAGTGCTTCCCAATCTTACTTGAATGTGCCACAAAGGCAATCAAAGCTGCTACAGATAAAGAATCAGAGGTACCCCATTTAAGTGGCATGGCTGTTGTGATATTGGAAGGATTGTTCTCTTCGCTTACTTACTTCTTCTCAAGAAATGGGTCTCATATGGTCGATTATCAGCTTGCTTTACAGAGATATGTTAAAAGAGATGTTGGACATTTTGGTGATTGGACACATACATTCAGTTTGTGGTGCTTGAATCCTGCTGTTGTGTTTCAAGATATCGCGAATCTTTCATTGTCAGTCATTTTAACATCAGGGACTTTGTCACCAATGAATTCCTTTTCATCTGAGCTTGGCCTTAAGTTTGGAAGTACTTTGGAAGCTCCCCATGTAATTGATGTTGAATCACAGGTTTGGCCTGCTATTATTTCTACTGGCCCACATAATGTGCCATTGAACTCAAGTTACAAGACAGCAGATGAATATGCCTTTCAGGATGCACTTGGAAAATCCTTAGAGGAAATTTTCAAAAGTGTGCCGGGTGGGTGTCTTGTGTTCTTCCCAAGTTACAAGCTAATGGGGAAGTTGGGCAAGCGTTGGCGTGAAACAGGACAATGGTCTCTGCTGAACGAAAGAAAGTCCCTTTTTGTTGAGCCCGGTGGAGGAGACCAGGAGGAATTTGAGTTTGTACTGAAGGGTTATTATGATTCAATTCGTCGGGGCAATAAGCCTACTTTTGGGAGAAATAAAGGTGCTAATAAGAAATTAACACAAAGTAGCTTCACTGCAGTCAAGTGTGCACAAAAGAATAACACTGATGGAGCTGCTTTACTGGCTGTGTGCCGAGGAAAGGTTTCAGAAGGAATCGACTTCACGGATGACAACGCTCGAGCTGTGATAATTGTGGGAATTCCATTTCCCAACATGAATGATATCAAGGTTTCCTTAAAGAAGAAATACAATGATACATATAGGTCATCCAAGGGCTTTCTGAGTGGAAATGAATGGTACTGTCACCAAGCCTTCCGAGCTTTAAATCAAGCTGCAGGACGTTGTATTCGACATAGATTCGATTATGGAGCTGTCATTTTGTTAGATGAACGTTATAAGGTAGGAAGAAATACAGCATATGTGTCAAAGTGGCTAAAGAAATCTATCAGACAATATGAAAGATTTGACATGTCAATGGAGGAATTAAGATCCTTTTTCAGCAATGTAAAGGAAAGGGTAAGCAAGAAAATGGTCAGTGTGTCACAGTGTTCTGACACTAGAAAGAAAATCATTCTTTCTATGGATCACGATAAAGGGTTTACAAGACTGAAAGATCAGAATTTGGATAAATCTGATCATCAAGGACAGTCAAAAACTTATGAAGGCAAGCACAATTCGATACTGACATCTCAAGGTGATCTTGATGTTCTGAGATTGTCACGAATGGATGTTTATGCTGACCATAGCAAGGACTTTATCAGTATTGAATCTACTCCTCAGAAATATACAAGGTACGCTAATCCATCTTTGTCCATCGCATTGTCTGATGCCGATCCAGACATCTCAATTGTCAAGGAAACCCCTTGTACGGATGGCATCTTTTCCACATGCAGTCCAGGATCAATTATTAAGGATGAGAAGTCCTGTTCGACTTCAACTACAGTTGAGTCCTCTCCTTTCTTTTCAGACCAGTTCTCATTTCAATCGACATCGTTAAACAATTGTAGCAATGCTTCTCTAAAGGCCCAGTGTTCTGTAGCCACCACTCCTGAAAAGAATGCAACTTTGAACTCTTACAGCTCAATGATGGAAACAGAATCATCTTTGGATATGAGTGTTAATTCTCATACCCAGAAAAGGAGAAAGTCCATGCATTCATCCTTGGTCAACTCCAGTCAGGGACAGTCTGATGGTGCTTCTGATTCTAAAACTCCTGATTGTCCTGGTTCCATAAAAGACACATTGACAAATAGAGATGCCATTCGCAAAATTGAATTCAGTGAGACTAATTATTCAGACCACTACTGTAAGTCCAAAGTTCATCATCCGCTCCCAAGTAGTTGTGGCGCTTTATCTATGTCAGCTGGTACTGTCATGGACAAAAGACTAGAAATTTGTTGCTCACTCTGCAAAAGCCCATTGGGCTTACCTGAGAATAATCTACATGTTACATGCTCAATAACTTCGTCATCAAAACTTCACTTAGAATCTCTTCATAACGAAATATTGAAGTCTCAAACTGGGAAGATATCAGCAGGCATACGCGTACTGATAACTGACATTTCATCAGTTGATTATCGAGTCTGCCATGAAATTTCTGAAGGTGCTCCTGGACAGGGTGTATGGTGTGATGAAGATGGGGGTGTTCTCGGTACCATCTTGTGCCCCTTCTGTCGGTACCACAAGACTAGCCACAATAATTTCCTTGGTGTACAGGTCATGGCAACTAATGCATCAAATGTTCATTTACTCAACAAGATAATGTTTTATTTAGATAAGTTGGAAATTCCAAATTTTGAAGCAACAAGTGACAAGGCTTCAAAAGACAAAGATTTCTTGCCTGTTAATAGTTCCAATGTCAACAAAGTTGCCATTCTCAATCCCTTTGAAAAATTCTCGTACTCTCCAGTACAGCAGGACCGAGGATGGAGGACTACAAAATCAAAGCTTAAACTACCCAAAAAAGCCTCACGTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003678 GO:0003824 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

DEAD_2 Helicase_C_2

Protein Analysis

1294

Amino Acids

144.22

Weight (kDa)

8.47

Isoelectric Point (pI)

42.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	168 - 331	5.6e-45	DEAD_2
Helicase_C_2	PF13307	570 - 781	6.1e-51	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20720 AT1G20720 AT1G20750
fragaria_vesca	FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570
malus_domestica	MD16G1283500.v1.1
prunus_persica	Prupe.5G009400_v2.0.a1 Prupe.5G009400_v2.0.a1 Prupe.5G009400_v2.0.a1
pyrus_communis	pycom16g26050
rosa_chinensis	RchiOBHm_Chr7g0215861
rosa_multiflora	Rmu_co7960896.1_g000001 Rmu_sc0004689.1_g000011 Rmu_sc0009085.1_g000007
rosa_roxburghii	Rroxscaffold_3G00243840
rosa_samantha	Rh7AG294400 Rh7CG314400 Rh7DG297200
rosa_wichuraiana	Rw7G024990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2298
Acc16I	TGCGCA	1 cut(s)	615
Acc65I	GGTACC	4 cut(s)	120, 1267, 3554, 3579
AccB1I	GGYRCC	5 cut(s)	120, 167, 1267, 3554, 3579
AccBSI	CCGCTC	1 cut(s)	3232
AccII	CGCG	2 cut(s)	1475, 3447
AccIII	TCCGGA	1 cut(s)	406
AciI	CCGC	4 cut(s)	124, 393, 1031, 3230
AclI	AACGTT	1 cut(s)	2293
AclWI	GGATC	6 cut(s)	675, 774, 2385, 2493, 2729, 2809
AcoI	YGGCCR	1 cut(s)	226
AcuI	CTGAAG	3 cut(s)	713, 1904, 3522
AcvI	CACGTG	1 cut(s)	3875
AcyI	GRCGYC	2 cut(s)	77, 168
AfiI	CCNNNNNNNGG	9 cut(s)	264, 1853, 1928, 2041, 2650, 2770, 3039, 3518, 3576
AflII	CTTAAG	2 cut(s)	1081, 1550
AflIII	ACRYGT	4 cut(s)	327, 2370, 3346, 3445
AgeI	ACCGGT	1 cut(s)	1151
AjnI	CCWGG	6 cut(s)	445, 906, 1860, 2797, 3124, 3511
AjuI	GAANNNNNNNTTGG	2 cut(s)	1094, 1126
AleI	CACNNNNGTG	1 cut(s)	1198
AloI	GAACNNNNNNTCC	4 cut(s)	387, 419, 1295, 1327
Alw21I	GWGCWC	3 cut(s)	430, 1993, 3511
Alw44I	GTGCAC	1 cut(s)	1989
AlwI	GGATC	6 cut(s)	675, 774, 2385, 2493, 2729, 2809
Ama87I	CYCGRG	2 cut(s)	245, 2079
Aor13HI	TCCGGA	1 cut(s)	406
ApaLI	GTGCAC	1 cut(s)	1989
ApeKI	GCWGC	8 cut(s)	304, 485, 741, 812, 1021, 1244, 2015, 2234
ArsI	GACNNNNNNTTYG	6 cut(s)	62, 94, 2232, 2264, 3279, 3311
AseI	ATTAAT	1 cut(s)	870
AsiGI	ACCGGT	1 cut(s)	1151
Asp700I	GAANNNNTTC	3 cut(s)	556, 2044, 3393
Asp718I	GGTACC	4 cut(s)	120, 1267, 3554, 3579
AspLEI	GCGC	3 cut(s)	170, 616, 3251
AspS9I	GGNCC	6 cut(s)	449, 1075, 1149, 1628, 2926, 3820
AsuC2I	CCSGG	5 cut(s)	90, 118, 265, 1731, 1848
AsuHPI	GGTGA	5 cut(s)	1166, 1424, 1509, 2198, 2609
AsuII	TTCGAA	1 cut(s)	438
AvaI	CYCGRG	2 cut(s)	245, 2079
AvaII	GGWCC	3 cut(s)	1075, 1149, 3820
BaeGI	GKGCMC	2 cut(s)	1993, 3569
BanI	GGYRCC	5 cut(s)	120, 167, 1267, 3554, 3579
BanII	GRGCYC	1 cut(s)	1848
BarI	GAAGNNNNNNTAC	2 cut(s)	2899, 2931
BauI	CACGAG	1 cut(s)	957
BbrPI	CACGTG	1 cut(s)	3875
Bbv12I	GWGCWC	3 cut(s)	430, 1993, 3511
BbvI	GCAGC	8 cut(s)	316, 497, 728, 799, 1033, 1231, 2002, 2221
BceAI	ACGGC	5 cut(s)	181, 241, 406, 436, 659
BciT130I	CCWGG	6 cut(s)	447, 908, 1862, 2799, 3126, 3513
BclI	TGATCA	1 cut(s)	2536
BcnI	CCSGG	5 cut(s)	90, 118, 265, 1731, 1848
BfaI	CTAG	5 cut(s)	102, 591, 2460, 3290, 3591
BfmI	CTRYAG	6 cut(s)	562, 1248, 1977, 2235, 2841, 2937
BfoI	RGCGCY	2 cut(s)	171, 3252
BfrI	CTTAAG	2 cut(s)	1081, 1550
BglI	GCCNNNNNGGC	3 cut(s)	176, 1232, 3324
BisI	GCNGC	8 cut(s)	305, 486, 742, 813, 1022, 1245, 2016, 2235
BlpI	GCTNAGC	1 cut(s)	429
BlsI	GCNGC	8 cut(s)	306, 487, 743, 814, 1023, 1246, 2017, 2236
BmcAI	AGTACT	1 cut(s)	1564
Bme18I	GGWCC	3 cut(s)	1075, 1149, 3820
BmeT110I	CYCGRG	2 cut(s)	245, 2079
BmgT120I	GGNCC	6 cut(s)	449, 1075, 1149, 1628, 2926, 3820
BmiI	GGNNCC	8 cut(s)	122, 169, 262, 889, 1269, 3130, 3556, 3581
BmrI	ACTGGG	2 cut(s)	2923, 3432
BmuI	ACTGGG	2 cut(s)	2923, 3432
BoxI	GACNNNNGTC	1 cut(s)	1807
BpmI	CTGGAG	3 cut(s)	1037, 3058, 3792
Bpu1102I	GCTNAGC	1 cut(s)	429
Bpu14I	TTCGAA	1 cut(s)	438
BpuEI	CTTGAG	5 cut(s)	45, 516, 1321, 1636, 2577
BpuMI	CCSGG	5 cut(s)	90, 118, 265, 1731, 1848
Bsa29I	ATCGAT	2 cut(s)	138, 639
BsaAI	YACGTR	1 cut(s)	3875
BsaBI	GATNNNNATC	1 cut(s)	2733
BsaHI	GRCGYC	2 cut(s)	77, 168
BsaI	GGTCTC	3 cut(s)	1353, 1815, 1851
BsaWI	WCCGGW	3 cut(s)	257, 406, 1151
BsaXI	ACNNNNNCTCC	2 cut(s)	387, 417
Bsc4I	CCNNNNNNNGG	9 cut(s)	264, 1853, 1928, 2041, 2650, 2770, 3039, 3518, 3576
Bse118I	RCCGGY	1 cut(s)	1151
Bse1I	ACTGG	9 cut(s)	875, 1020, 1630, 2028, 2872, 2929, 3075, 3427, 3809
Bse3DI	GCAATG	2 cut(s)	2410, 2917
Bse8I	GATNNNNATC	1 cut(s)	2733
BseAI	TCCGGA	1 cut(s)	406
BseBI	CCWGG	6 cut(s)	447, 908, 1862, 2799, 3126, 3513
BseCI	ATCGAT	2 cut(s)	138, 639
BseJI	GATNNNNATC	1 cut(s)	2733
BseLI	CCNNNNNNNGG	9 cut(s)	264, 1853, 1928, 2041, 2650, 2770, 3039, 3518, 3576
BseMI	GCAATG	2 cut(s)	2410, 2917
BseMII	CTCAG	7 cut(s)	443, 470, 1530, 2174, 2603, 2693, 3324
BseNI	ACTGG	9 cut(s)	875, 1020, 1630, 2028, 2872, 2929, 3075, 3427, 3809
BseRI	GAGGAG	3 cut(s)	428, 1869, 2667
BseSI	GKGCMC	2 cut(s)	1993, 3569
BseXI	GCAGC	8 cut(s)	316, 497, 728, 799, 1033, 1231, 2002, 2221
BsgI	GTGCAG	1 cut(s)	589
Bsh1236I	CGCG	2 cut(s)	1475, 3447
BshNI	GGYRCC	5 cut(s)	120, 167, 1267, 3554, 3579
BshTI	ACCGGT	1 cut(s)	1151
BshVI	ATCGAT	2 cut(s)	138, 639
BsiHKAI	GWGCWC	3 cut(s)	430, 1993, 3511
BsiHKCI	CYCGRG	2 cut(s)	245, 2079
BsiSI	CCGG	8 cut(s)	89, 118, 258, 264, 407, 1152, 1730, 1848
BslFI	GGGAC	4 cut(s)	399, 1522, 1816, 3096
BslI	CCNNNNNNNGG	9 cut(s)	264, 1853, 1928, 2041, 2650, 2770, 3039, 3518, 3576
BsmBI	CGTCTC	2 cut(s)	202, 459
BsmFI	GGGAC	4 cut(s)	399, 1522, 1816, 3096
BsmI	GAATGC	2 cut(s)	2965, 3055
Bso31I	GGTCTC	3 cut(s)	1353, 1815, 1851
BsoBI	CYCGRG	2 cut(s)	245, 2079
Bsp119I	TTCGAA	1 cut(s)	438
Bsp1286I	GDGCHC	5 cut(s)	430, 1848, 1993, 3511, 3569
Bsp13I	TCCGGA	1 cut(s)	406
Bsp1407I	TGTACA	3 cut(s)	330, 700, 3613
Bsp143I	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
Bsp1720I	GCTNAGC	1 cut(s)	429
Bsp19I	CCATGG	1 cut(s)	891
Bsp68I	TCGCGA	1 cut(s)	1475
BspACI	CCGC	4 cut(s)	124, 393, 1031, 3230
BspCNI	CTCAG	7 cut(s)	442, 469, 1531, 2175, 2604, 2692, 3325
BspDI	ATCGAT	2 cut(s)	138, 639
BspEI	TCCGGA	1 cut(s)	406
BspFNI	CGCG	2 cut(s)	1475, 3447
BspHI	TCATGA	1 cut(s)	183
BspLI	GGNNCC	8 cut(s)	122, 169, 262, 889, 1269, 3130, 3556, 3581
BspMAI	CTGCAG	2 cut(s)	1981, 2239
BspPI	GGATC	6 cut(s)	675, 774, 2385, 2493, 2729, 2809
BspT104I	TTCGAA	1 cut(s)	438
BspT107I	GGYRCC	5 cut(s)	120, 167, 1267, 3554, 3579
BspTI	CTTAAG	2 cut(s)	1081, 1550
BspTNI	GGTCTC	3 cut(s)	1353, 1815, 1851
BsrBI	CCGCTC	1 cut(s)	3232
BsrDI	GCAATG	2 cut(s)	2410, 2917
BsrFI	RCCGGY	1 cut(s)	1151
BsrGI	TGTACA	3 cut(s)	330, 700, 3613
BsrI	ACTGG	9 cut(s)	875, 1020, 1630, 2028, 2872, 2929, 3075, 3427, 3809
BssAI	RCCGGY	1 cut(s)	1151
BssMI	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
BssNI	GRCGYC	2 cut(s)	77, 168
BssSI	CACGAG	1 cut(s)	957
BssT1I	CCWWGG	6 cut(s)	363, 381, 891, 2172, 3063, 3607
Bst2BI	CACGAG	1 cut(s)	957
Bst2UI	CCWGG	6 cut(s)	447, 908, 1862, 2799, 3126, 3513
Bst4CI	ACNGT	8 cut(s)	54, 2204, 2448, 2550, 2845, 3087, 3209, 3274
Bst6I	CTCTTC	6 cut(s)	39, 46, 405, 732, 1321, 3399
BstACI	GRCGYC	2 cut(s)	77, 168
BstAFI	CTTAAG	2 cut(s)	1081, 1550
BstAPI	GCANNNNNTGC	1 cut(s)	3632
BstAUI	TGTACA	3 cut(s)	330, 700, 3613
BstBAI	YACGTR	1 cut(s)	3875
BstBI	TTCGAA	1 cut(s)	438
BstC8I	GCNNGC	6 cut(s)	1031, 1371, 1611, 1784, 2570, 3439
BstDEI	CTNAG	9 cut(s)	429, 456, 1539, 1705, 2183, 2612, 2679, 3333, 3385
BstDSI	CCRYGG	1 cut(s)	891
BstFNI	CGCG	2 cut(s)	1475, 3447
BstH2I	RGCGCY	2 cut(s)	171, 3252
BstHHI	GCGC	3 cut(s)	170, 616, 3251
BstKTI	GATC	9 cut(s)	670, 769, 2393, 2488, 2518, 2539, 2602, 2737, 2804
BstMBI	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
BstNI	CCWGG	6 cut(s)	447, 908, 1862, 2799, 3126, 3513
BstNSI	RCATGY	5 cut(s)	331, 2374, 2793, 3350, 3357
BstPAI	GACNNNNGTC	1 cut(s)	1807
BstSFI	CTRYAG	6 cut(s)	562, 1248, 1977, 2235, 2841, 2937
BstSLI	GKGCMC	2 cut(s)	1993, 3569
BstUI	CGCG	2 cut(s)	1475, 3447
BstV1I	GCAGC	8 cut(s)	316, 497, 728, 799, 1033, 1231, 2002, 2221
BstX2I	RGATCY	2 cut(s)	766, 2390
BstXI	CCANNNNNNTGG	1 cut(s)	1280
BstYI	RGATCY	2 cut(s)	766, 2390
Bsu15I	ATCGAT	2 cut(s)	138, 639
BsuTUI	ATCGAT	2 cut(s)	138, 639
BtgI	CCRYGG	1 cut(s)	891
BtgZI	GCGATG	1 cut(s)	2701
BtsI	GCAGTG	4 cut(s)	229, 585, 1205, 1974
BtsIMutI	CAGTG	9 cut(s)	229, 585, 1205, 1974, 2004, 2443, 2453, 2936, 3187
BtuMI	TCGCGA	1 cut(s)	1475
Cac8I	GCNNGC	6 cut(s)	1031, 1371, 1611, 1784, 2570, 3439
CciI	TCATGA	1 cut(s)	183
CfoI	GCGC	3 cut(s)	170, 616, 3251
Cfr10I	RCCGGY	1 cut(s)	1151
Cfr13I	GGNCC	6 cut(s)	449, 1075, 1149, 1628, 2926, 3820
ClaI	ATCGAT	2 cut(s)	138, 639
CseI	GACGC	1 cut(s)	66
CspAI	ACCGGT	1 cut(s)	1151
DdeI	CTNAG	9 cut(s)	429, 456, 1539, 1705, 2183, 2612, 2679, 3333, 3385
DinI	GGCGCC	1 cut(s)	169
DpnI	GATC	9 cut(s)	669, 768, 2392, 2487, 2517, 2538, 2601, 2736, 2803
DpnII	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
DraI	TTTAAA	2 cut(s)	694, 2226
EaeI	YGGCCR	1 cut(s)	226
Eam1104I	CTCTTC	6 cut(s)	39, 46, 405, 732, 1321, 3399
EarI	CTCTTC	6 cut(s)	39, 46, 405, 732, 1321, 3399
EciI	GGCGGA	1 cut(s)	408
Eco130I	CCWWGG	6 cut(s)	363, 381, 891, 2172, 3063, 3607
Eco147I	AGGCCT	1 cut(s)	752
Eco24I	GRGCYC	1 cut(s)	1848
Eco31I	GGTCTC	3 cut(s)	1353, 1815, 1851
Eco32I	GATATC	4 cut(s)	904, 1471, 2125, 3432
Eco47I	GGWCC	3 cut(s)	1075, 1149, 3820
Eco57I	CTGAAG	3 cut(s)	713, 1904, 3522
Eco72I	CACGTG	1 cut(s)	3875
Eco88I	CYCGRG	2 cut(s)	245, 2079
EcoO109I	RGGNCCY	1 cut(s)	1075
EcoRI	GAATTC	3 cut(s)	1525, 2100, 3176
EcoRII	CCWGG	6 cut(s)	445, 906, 1860, 2797, 3124, 3511
EcoRV	GATATC	4 cut(s)	904, 1471, 2125, 3432
EcoT14I	CCWWGG	6 cut(s)	363, 381, 891, 2172, 3063, 3607
EcoT22I	ATGCAT	3 cut(s)	508, 3057, 3637
EcoT38I	GRGCYC	1 cut(s)	1848
EgeI	GGCGCC	1 cut(s)	169
EheI	GGCGCC	1 cut(s)	169
ErhI	CCWWGG	6 cut(s)	363, 381, 891, 2172, 3063, 3607
Esp3I	CGTCTC	2 cut(s)	202, 459
FalI	AAGNNNNNCTT	4 cut(s)	2120, 2152, 2586, 2618
FaqI	GGGAC	4 cut(s)	399, 1522, 1816, 3096
FauI	CCCGC	1 cut(s)	1038
FauNDI	CATATG	2 cut(s)	1353, 2320
FbaI	TGATCA	1 cut(s)	2536
Fnu4HI	GCNGC	8 cut(s)	305, 486, 742, 813, 1022, 1245, 2016, 2235
FriOI	GRGCYC	1 cut(s)	1848
Fsp4HI	GCNGC	8 cut(s)	305, 486, 742, 813, 1022, 1245, 2016, 2235
FspBI	CTAG	5 cut(s)	102, 591, 2460, 3290, 3591
FspI	TGCGCA	1 cut(s)	615
GlaI	GCGC	3 cut(s)	169, 615, 3250
GluI	GCNGC	8 cut(s)	305, 486, 742, 813, 1022, 1245, 2016, 2235
GsuI	CTGGAG	3 cut(s)	1037, 3058, 3792
HaeII	RGCGCY	2 cut(s)	171, 3252
HapII	CCGG	8 cut(s)	89, 118, 258, 264, 407, 1152, 1730, 1848
HgaI	GACGC	1 cut(s)	66
HhaI	GCGC	3 cut(s)	170, 616, 3251
Hin1I	GRCGYC	2 cut(s)	77, 168
Hin6I	GCGC	3 cut(s)	168, 614, 3249
HinP1I	GCGC	3 cut(s)	168, 614, 3249
HincII	GTYRAC	2 cut(s)	3070, 3763
HindII	GTYRAC	2 cut(s)	3070, 3763
HindIII	AAGCTT	1 cut(s)	3848
HpaII	CCGG	8 cut(s)	89, 118, 258, 264, 407, 1152, 1730, 1848
HphI	GGTGA	5 cut(s)	1166, 1424, 1509, 2198, 2609
Hpy166II	GTNNAC	8 cut(s)	333, 703, 1048, 1991, 2502, 3070, 3614, 3763
Hpy8I	GTNNAC	8 cut(s)	333, 703, 1048, 1991, 2502, 3070, 3614, 3763
Hpy99I	CGWCG	2 cut(s)	227, 1911
HpyCH4III	ACNGT	8 cut(s)	54, 2204, 2448, 2550, 2845, 3087, 3209, 3274
HpyCH4IV	ACGT	4 cut(s)	1120, 2242, 2293, 3874
HpyF3I	CTNAG	9 cut(s)	429, 456, 1539, 1705, 2183, 2612, 2679, 3333, 3385
HpySE526I	ACGT	4 cut(s)	1120, 2242, 2293, 3874
Hsp92I	GRCGYC	2 cut(s)	77, 168
HspAI	GCGC	3 cut(s)	168, 614, 3249
KasI	GGCGCC	1 cut(s)	167
Kpn2I	TCCGGA	1 cut(s)	406
KpnI	GGTACC	4 cut(s)	124, 1271, 3558, 3583
Ksp22I	TGATCA	1 cut(s)	2536
Kzo9I	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
LmnI	GCTCC	8 cut(s)	493, 887, 1018, 1579, 2012, 2270, 3237, 3514
Lsp1109I	GCAGC	8 cut(s)	316, 497, 728, 799, 1033, 1231, 2002, 2221
MaeI	CTAG	5 cut(s)	102, 591, 2460, 3290, 3591
MaeII	ACGT	4 cut(s)	1120, 2242, 2293, 3874
MalI	GATC	9 cut(s)	669, 768, 2392, 2487, 2517, 2538, 2601, 2736, 2803
MbiI	CCGCTC	1 cut(s)	3232
MboI	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
MfeI	CAATTG	2 cut(s)	2748, 2902
MflI	RGATCY	2 cut(s)	766, 2390
MhlI	GDGCHC	5 cut(s)	430, 1848, 1993, 3511, 3569
MluI	ACGCGT	1 cut(s)	3445
Mly113I	GGCGCC	1 cut(s)	168
MlyI	GAGTC	4 cut(s)	201, 257, 2858, 3492
MmeI	TCCRAC	7 cut(s)	658, 760, 1067, 1092, 1127, 1381, 3663
Mph1103I	ATGCAT	3 cut(s)	508, 3057, 3637
MroI	TCCGGA	1 cut(s)	406
MroXI	GAANNNNTTC	3 cut(s)	556, 2044, 3393
MslI	CAYNNNNRTG	4 cut(s)	1198, 1278, 1410, 2375
MspA1I	CMGCKG	2 cut(s)	1033, 3266
MspCI	CTTAAG	2 cut(s)	1081, 1550
MspI	CCGG	8 cut(s)	89, 118, 258, 264, 407, 1152, 1730, 1848
MunI	CAATTG	2 cut(s)	2748, 2902
Mva1269I	GAATGC	2 cut(s)	2965, 3055
MvaI	CCWGG	6 cut(s)	447, 908, 1862, 2799, 3126, 3513
MvnI	CGCG	2 cut(s)	1475, 3447
NarI	GGCGCC	1 cut(s)	168
NciI	CCSGG	5 cut(s)	90, 118, 265, 1731, 1848
NcoI	CCATGG	1 cut(s)	891
NdeI	CATATG	2 cut(s)	1353, 2320
NdeII	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
NlaIV	GGNNCC	8 cut(s)	122, 169, 262, 889, 1269, 3130, 3556, 3581
NmeAIII	GCCGAG	1 cut(s)	2060
NmuCI	GTSAC	6 cut(s)	1154, 1515, 2204, 2442, 2619, 3710
NruI	TCGCGA	1 cut(s)	1475
NsbI	TGCGCA	1 cut(s)	615
NsiI	ATGCAT	3 cut(s)	508, 3057, 3637
NspI	RCATGY	5 cut(s)	331, 2374, 2793, 3350, 3357
NspV	TTCGAA	1 cut(s)	438
OliI	CACNNNNGTG	1 cut(s)	1198
PaeR7I	CTCGAG	2 cut(s)	245, 2079
PagI	TCATGA	1 cut(s)	183
PceI	AGGCCT	1 cut(s)	752
PciI	ACATGT	3 cut(s)	327, 2370, 3346
PcsI	WCGNNNNNNNCGW	3 cut(s)	135, 243, 2248
PctI	GAATGC	2 cut(s)	2965, 3055
PdmI	GAANNNNTTC	3 cut(s)	556, 2044, 3393
PflFI	GACNNNGTC	1 cut(s)	1513
PfoI	TCCNGGA	3 cut(s)	88, 2797, 3511
PinAI	ACCGGT	1 cut(s)	1151
PkrI	GCNGC	8 cut(s)	306, 487, 743, 814, 1023, 1246, 2017, 2236
PleI	GAGTC	4 cut(s)	200, 256, 2857, 3491
PluTI	GGCGCC	1 cut(s)	171
PmaCI	CACGTG	1 cut(s)	3875
PmlI	CACGTG	1 cut(s)	3875
PpsI	GAGTC	4 cut(s)	200, 256, 2857, 3491
Ppu21I	YACGTR	1 cut(s)	3875
PpuMI	RGGWCCY	1 cut(s)	1075
PscI	ACATGT	3 cut(s)	327, 2370, 3346
PshAI	GACNNNNGTC	1 cut(s)	1807
PshBI	ATTAAT	1 cut(s)	870
PsiI	TTATAA	1 cut(s)	2298
Psp1406I	AACGTT	1 cut(s)	2293
Psp5II	RGGWCCY	1 cut(s)	1075
Psp6I	CCWGG	6 cut(s)	445, 906, 1860, 2797, 3124, 3511
PspCI	CACGTG	1 cut(s)	3875
PspGI	CCWGG	6 cut(s)	445, 906, 1860, 2797, 3124, 3511
PspN4I	GGNNCC	8 cut(s)	122, 169, 262, 889, 1269, 3130, 3556, 3581
PspPI	GGNCC	6 cut(s)	449, 1075, 1149, 1628, 2926, 3820
PspPPI	RGGWCCY	1 cut(s)	1075
PspXI	VCTCGAGB	2 cut(s)	245, 2079
PsrI	GAACNNNNNNTAC	2 cut(s)	1640, 1672
PstI	CTGCAG	2 cut(s)	1981, 2239
PsuI	RGATCY	2 cut(s)	766, 2390
PsyI	GACNNNGTC	1 cut(s)	1513
PvuII	CAGCTG	1 cut(s)	3266
RruI	TCGCGA	1 cut(s)	1475
RseI	CAYNNNNRTG	4 cut(s)	1198, 1278, 1410, 2375
SatI	GCNGC	8 cut(s)	305, 486, 742, 813, 1022, 1245, 2016, 2235
Sau3AI	GATC	9 cut(s)	667, 766, 2390, 2485, 2515, 2536, 2599, 2734, 2801
Sau96I	GGNCC	6 cut(s)	449, 1075, 1149, 1628, 2926, 3820
ScaI	AGTACT	1 cut(s)	1564
SchI	GAGTC	4 cut(s)	201, 257, 2858, 3492
SduI	GDGCHC	5 cut(s)	430, 1848, 1993, 3511, 3569
SfcI	CTRYAG	6 cut(s)	562, 1248, 1977, 2235, 2841, 2937
SfoI	GGCGCC	1 cut(s)	169
Sfr274I	CTCGAG	2 cut(s)	245, 2079
SfuI	TTCGAA	1 cut(s)	438
SinI	GGWCC	3 cut(s)	1075, 1149, 3820
SlaI	CTCGAG	2 cut(s)	245, 2079
SmiMI	CAYNNNNRTG	4 cut(s)	1198, 1278, 1410, 2375
SmlI	CTYRAG	9 cut(s)	60, 245, 531, 1081, 1336, 1550, 1651, 2079, 2592
SmoI	CTYRAG	9 cut(s)	60, 245, 531, 1081, 1336, 1550, 1651, 2079, 2592
SseBI	AGGCCT	1 cut(s)	752
SsiI	CCGC	4 cut(s)	124, 393, 1031, 3230
SspDI	GGCGCC	1 cut(s)	167
SspI	AATATT	1 cut(s)	3408
SspMI	CTAG	5 cut(s)	102, 591, 2460, 3290, 3591
StuI	AGGCCT	1 cut(s)	752
StyI	CCWWGG	6 cut(s)	363, 381, 891, 2172, 3063, 3607
TaaI	ACNGT	8 cut(s)	54, 2204, 2448, 2550, 2845, 3087, 3209, 3274
TaiI	ACGT	4 cut(s)	1123, 2245, 2296, 3877
TaqII	GACCGA	2 cut(s)	389, 3837
TatI	WGTACW	7 cut(s)	330, 619, 700, 1562, 1879, 3613, 3811
TscAI	CASTG	9 cut(s)	236, 585, 1205, 1981, 2011, 2443, 2453, 2936, 3187
TseFI	GTSAC	6 cut(s)	1154, 1515, 2204, 2442, 2619, 3710
TseI	GCWGC	8 cut(s)	304, 485, 741, 812, 1021, 1244, 2015, 2234
Tsp45I	GTSAC	6 cut(s)	1154, 1515, 2204, 2442, 2619, 3710
TspGWI	ACGGA	3 cut(s)	530, 2081, 2786
TspRI	CASTG	9 cut(s)	236, 585, 1205, 1981, 2011, 2443, 2453, 2936, 3187
Tth111I	GACNNNGTC	1 cut(s)	1513
Vha464I	CTTAAG	2 cut(s)	1081, 1550
VneI	GTGCAC	1 cut(s)	1989
VpaK11BI	GGWCC	3 cut(s)	1075, 1149, 3820
VspI	ATTAAT	1 cut(s)	870
XceI	RCATGY	5 cut(s)	331, 2374, 2793, 3350, 3357
XhoI	CTCGAG	2 cut(s)	245, 2079
XmnI	GAANNNNTTC	3 cut(s)	556, 2044, 3393
XspI	CTAG	5 cut(s)	102, 591, 2460, 3290, 3591
ZrmI	AGTACT	1 cut(s)	1564
Zsp2I	ATGCAT	3 cut(s)	508, 3057, 3637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.