Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0004306

Physical Location & Seq

Genomic Coordinates Reverse (-)

32190087 .. 32200520

Gene Structure 10434 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG294400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3804 bp

ATGGCGTGTGCAATTCCGGGAGCAAACCCTAAAAGCTCATCGAAAAGCTCGTACCGCATCGGAGGCATCGATGTGGAGTTCCCGTACCAGCCGTACGCGGCGCAGATGGCGTTCATGAGCAGAGTCGTCTCCACTCTCGATCGAGCTGTCAGGGACGACCACTGCCACGCCTTGCTCGAGTCTCCCACCGGAACCGGCAAATCGCTCTCTCTTCTCTGCTCCATCCTCGCCTGGCAGCAGAGCTTCAAGGCCAAGAATATGTACGCCAATCTCTCCCATTCCAAGCCCAACCCCAAGGCCATGACCGATCCCATCGCCCACGGCGGCGGCTTTGTTCCCGAAGAGGAGCACACCAGTGCTCAGCCATCGGAAAAGCCAGGGCCGTCTCAATCTGAAAATCGGAAGAAAAAGGCAGCTCCTACCATATACTATGCATCGAGGACACATTCACAAATTTCTCAAGTCATCCGTGAATATCGGAAAACTTCCTACCGGGTGCCTATGGCAGTGCTGGCCTCTAGGAAGCATTACTGCACAAATGCACAAGTACATGGGAGAGAGAATATTGACGAAGAATGTAAGCTTCTTTTGAAGGATCAAGTGGTTGGATGCGTAGAATTTAAAAACGTACACAAAGTCAAAGGACATCCATCTCTTCAGAAAGGAGGCTGCCACGAGGCCTACGATATTGAGGATCTCGTGAAAGTTGGAAAAGTTGTCAAAGGTTGTTCATATTATGCTGCTCGTTCTATGTCAGACGATGCAGAACTGGTTTTTTGTCCATATAACTACATCATTAATCCAGTCATTAGGGGAGCCATGGAAGTAGATATTCAGGGGTCCATTGTAGTTCTGGATGAAGCCCACAATATAGAGGATGTTGCTCGCGATGCTGGTAGTGTAGATATTGATGAAGAAGTTTTGAACAAATTGCAGATGGAACTAGAGCAGCTTTGCCCACTGGATGTTTTGGTTTACCAACCTCTGTATGAAATGATACAGGACCTCTTAAGTTGGATTGAGCGAAGGAAAGACAAGTTAGAAAAGCGTGAATTTCAGCACTTTGTGTCTTGTTGGACTGGTGACAAGGCTTTGAGAGAGCTTCAGGAAGCTAATATATCACAGCAGTGCTTCCCAGTCTTACTTGAATGTGCCACAAAGGCAATCAAAGCTGCTACAGATAAAGAATCAGAGGTAGCCCATTTAAGTGGCATGGCTGTTGTGACATTGGAAGGATTGTTCTCTTCACTTACTTACTTCTTCTCAAGAAATGGGTCTCATATGGTCGATTATCAGCTTGCTTTACAGAGATATGTTAAAAGAGATGTTGGAACTTTTGGCGATTGGACACATACATTCAGTTTGTGGTGCTTGAATCCTGCTGTTGTGTTTCAAGATATTGCCAATCTTTCCTTGTCAGTCATTTTAACATCGGGAACTTTGTCACCAATGAATTCCTTTTCATCTGAGCTTGGCCTTCAGTTTGGAAGTACTCTGGAAGCTCCCCATGTAATTGATGTTGAATCACAGGTTTGGCCCACTATTATTTCTACTGGCCCAGGTAATGTGCCATTGAATGCAAGTTATAAGACAGCAGATGAATATGCCTTCCAGGATGCACTTGGAAAATCCTTAGAGGAAATTTTCAAAAGTGTGCCGGCTGGGTGTCTTGTCTTCTTCCCAAGTTACAAGCTAATGGGGAAGTTATGCAAGCGTTGGCGTGAAACAGGACAATGGTCTCTGCTAAACGAAAGAAAGTCCCTTTTTATTGAGCCCGGTGGAGACCAGGAGGAATTTGAGTTTGTACTGAAGGGTTATTATGATTCAATTCGTGGGGGCAATAAGCCTGCTTTTGGGAGAAATAAAGGTGCTAACAAGAAATTAAGACAAAGTAGCTTCACTGCAGTCAAGTGTGCACAAAAGAATAACACTGATGGAGCTGCTTTACTGGCTGTGTGCCGAGGAAAGGTTTCAGAAGGAATCGACTTCACAGATGACAATGCTCGGGCTGTGATAATTGTGGGAATTCCATTTCCCAACATGAATGACATTAAGATTTCCTTAAAGAAGAAATACAATGATGCATATAAGTCATCCAAGGGCTTTCTGAGTGGAAATGAATGGTACTGTCACCAAGCCTTCCGAGCTTTAAATCAAGCTGCAGGACGTTGTATTCGACATAGGTTTGATTATGGAGCTGTCATCTTATTAGATGAACGTTATCAGGTAGGAAGAAATACAGCATATGTGTCAAAGTGGCTAAAGAAATCCATTAGACAGTATGAAAGCTTTGACACGTCAATAGAGGAATTAAAATCCTTTTTCAACAATGTCAAGGAAAGGGTAAGCAAGAATATGGTCAATGTGTCACAGAGTTCTGAGACTAGAGAGAAAATCATTCTTTCTATGGATCACAGTAAAGGGTTTACAAGACTGAAAGATCAGAATTTGAATAAATCTGATCCTCAGGGACAGACAAAAACTTATGTAGGCAAGCACGATTCTCTACTGACATCTCAAGATGATCTTGATGTATCATCACGAATGGATGTATATGCTGACAATAGCAAGGACTTTATCAATATCAGATCTACTCCTCAGAAAGATACAAGGTACCTTAAGCCATCTTTGTCCATGTCATTCTCTGATGCAGATCCAGACATCTCAGTTGTCAATGAAACCCCTTGTATGGATGGCATCTTTTCCACGTGCAGTCCAGGATCAGTTACTAAGGATGAGAAGTGCTGTTCGACTTCGACTACAATTGAGTCCTCTTCTTTCTTTTTGGACCAATTCTCATTCCAATCAACATCATTAACCAATTGTAGCAAGGCTTCTCCAAAGGGCCAGTGTTCTGTAGCCGCTACTCCTGAAAAGAATGCAACTTTGAACTCTTACAGCTCAATGACAGAAACAGAATCATCTTTGGATTTGAGTGTTAATTCTCATATCCAGAAAAGGAGAAAGTCCATATATCAATCCTTGGTTAACTCCAGTCAGGGACAGTCTGGTGGTGCTCCTGATTCTGAAACTTCTGATTGTCCTGATTCCGTAAAAGAGACAATGACCAGTGGAGATGCCATTCGCAAAATTGAATTTAATGAATCTAATTATTCAGAGCACTATTGTAAGTCCATAGTTCATCATCTTCCCCCGAGTACTTGTGGCACTTCATCTGTGTCAGTTGGTTCTGTCATGGACAAGAGACTAGAAATCTGTTGCTCACTCTGCAAAAGCCCATTGGGCCTTCCTGAGAATAATCTACATGTTACATGCTCAATAACTTCATCATCGAAACTTTACTTGGAATCTCTTCATAACGAAATATTGAAATCTCAAACTGGGAATACATCAGCAGGCATACGTGTTCTCATAACTGACATTTCATCAGTTGATTATCGAGTCTGCCATGAAATTTCTGAAGGCACTCCTGGACAGGGTGTATGGTGTGAAAAAGATGGGTTTGTTTTCGGTACCATCTTGTGCCCCTTTTGCCGTCACCACAAGAGTAGCCACGATAATTTCCTTGGCGTACAGGTCATGGCAACTAATGCATCAAATGTTCATTTACTCAACAAGATCATGTTTTACTTAGATCAGTTGGAAATTACAAATTTTGAGGCATCTTCTGACAAGGCTACAAAAGACAAAGATTTCTTGCCTGTTAATGGTTCCAACGTCGACAAAGTTGCCATTCTCAATCCCTTTGATAAATTCTCATACTCTCCAGTACAGCAGGACCAAGGAGGATGGAGGACTACAAAATCAAAGCTTAAACTACCCAAAAGACCCTCACGTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003678 GO:0003824 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

DEAD_2 Helicase_C_2

Protein Analysis

1267

Amino Acids

140.82

Weight (kDa)

7.01

Isoelectric Point (pI)

44.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	144 - 307	3.4e-44	DEAD_2
Helicase_C_2	PF13307	546 - 756	5.2e-51	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20720 AT1G20720 AT1G20750
fragaria_vesca	FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570
malus_domestica	MD16G1283500.v1.1
prunus_persica	Prupe.5G009400_v2.0.a1 Prupe.5G009400_v2.0.a1 Prupe.5G009400_v2.0.a1
pyrus_communis	pycom16g26050
rosa_chinensis	RchiOBHm_Chr7g0215861
rosa_multiflora	Rmu_co7960896.1_g000001 Rmu_sc0004689.1_g000011 Rmu_sc0009085.1_g000007
rosa_roxburghii	Rroxscaffold_3G00243840
rosa_samantha	Rh7AG294400 Rh7CG314400 Rh7DG297200
rosa_wichuraiana	Rw7G024990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1587
Acc65I	GGTACC	2 cut(s)	2613, 3473
AccB1I	GGYRCC	3 cut(s)	496, 2613, 3473
AccI	GTMKAC	1 cut(s)	3681
AccII	CGCG	2 cut(s)	98, 888
AciI	CCGC	5 cut(s)	55, 98, 324, 327, 2862
AclI	AACGTT	1 cut(s)	2218
AclWI	GGATC	7 cut(s)	302, 603, 702, 2418, 2456, 2648, 2728
AcuI	CTGAAG	5 cut(s)	641, 1088, 1463, 1829, 3441
AcvI	CACGTG	1 cut(s)	2709
AdeI	CACNNNGTG	1 cut(s)	1066
AfiI	CCNNNNNNNGG	5 cut(s)	1853, 1966, 2689, 3437, 3668
AflII	CTTAAG	2 cut(s)	1009, 2618
AflIII	ACRYGT	3 cut(s)	2295, 3265, 3364
AjiI	CACGTC	1 cut(s)	2298
AjnI	CCWGG	7 cut(s)	230, 376, 1558, 1611, 1785, 2716, 3430
AjuI	GAANNNNNNNTTGG	2 cut(s)	3287, 3319
AleI	CACNNNNGTG	2 cut(s)	354, 1126
AloI	GAACNNNNNNTCC	2 cut(s)	1223, 1255
Alw21I	GWGCWC	5 cut(s)	351, 361, 1918, 3019, 3123
Alw26I	GTCTC	9 cut(s)	133, 186, 390, 1281, 1743, 1776, 2375, 3053, 3199
Alw44I	GTGCAC	1 cut(s)	1914
AlwI	GGATC	7 cut(s)	302, 603, 702, 2418, 2456, 2648, 2728
Ama87I	CYCGRG	3 cut(s)	176, 2004, 3154
ApaLI	GTGCAC	1 cut(s)	1914
ApeKI	GCWGC	8 cut(s)	235, 413, 669, 740, 949, 1172, 1940, 2159
ArsI	GACNNNNNNTTYG	2 cut(s)	2157, 2189
AseI	ATTAAT	1 cut(s)	798
Asp700I	GAANNNNTTC	2 cut(s)	1969, 3312
Asp718I	GGTACC	2 cut(s)	2613, 3473
AspLEI	GCGC	1 cut(s)	103
AspS9I	GGNCC	9 cut(s)	380, 840, 1003, 1536, 1556, 2788, 2845, 3244, 3739
AsuC2I	CCSGG	3 cut(s)	18, 494, 1776
AsuHPI	GGTGA	4 cut(s)	1094, 1437, 2123, 3491
AvaI	CYCGRG	3 cut(s)	176, 2004, 3154
AvaII	GGWCC	4 cut(s)	840, 1003, 2788, 3739
AxyI	CCTNAGG	1 cut(s)	2466
BaeGI	GKGCMC	2 cut(s)	1918, 3488
BanI	GGYRCC	3 cut(s)	496, 2613, 3473
BanII	GRGCYC	1 cut(s)	1776
BarI	GAAGNNNNNNTAC	2 cut(s)	2818, 2850
BauI	CACGAG	2 cut(s)	674, 698
BbrPI	CACGTG	1 cut(s)	2709
BbsI	GAAGAC	1 cut(s)	1666
Bbv12I	GWGCWC	5 cut(s)	351, 361, 1918, 3019, 3123
BbvI	GCAGC	8 cut(s)	247, 425, 656, 727, 961, 1159, 1927, 2146
BceAI	ACGGC	4 cut(s)	76, 337, 367, 3480
BcgI	CGANNNNNNTGC	2 cut(s)	3671, 3705
BciT130I	CCWGG	7 cut(s)	232, 378, 1560, 1613, 1787, 2718, 3432
BcnI	CCSGG	3 cut(s)	18, 494, 1776
BcoDI	GTCTC	9 cut(s)	133, 186, 390, 1281, 1743, 1776, 2375, 3053, 3199
BfaI	CTAG	4 cut(s)	519, 944, 2385, 3209
BfmI	CTRYAG	4 cut(s)	1176, 1902, 2160, 2856
BfrI	CTTAAG	2 cut(s)	1009, 2618
BglI	GCCNNNNNGGC	2 cut(s)	1160, 3243
BglII	AGATCT	1 cut(s)	2588
BlpI	GCTNAGC	1 cut(s)	360
BmcAI	AGTACT	2 cut(s)	1492, 3160
Bme18I	GGWCC	4 cut(s)	840, 1003, 2788, 3739
BmeT110I	CYCGRG	3 cut(s)	176, 2004, 3154
BmgBI	CACGTC	1 cut(s)	2298
BmgT120I	GGNCC	9 cut(s)	380, 840, 1003, 1536, 1556, 2788, 2845, 3244, 3739
BmiI	GGNNCC	7 cut(s)	193, 498, 817, 841, 2615, 3475, 3673
BmrI	ACTGGG	2 cut(s)	1130, 3351
BmuI	ACTGGG	2 cut(s)	1130, 3351
BoxI	GACNNNNGTC	1 cut(s)	1735
BpiI	GAAGAC	1 cut(s)	1666
BpmI	CTGGAG	2 cut(s)	2977, 3711
Bpu1102I	GCTNAGC	1 cut(s)	360
BpuEI	CTTGAG	3 cut(s)	444, 1249, 2502
BpuMI	CCSGG	3 cut(s)	18, 494, 1776
Bsa29I	ATCGAT	1 cut(s)	69
BsaAI	YACGTR	2 cut(s)	2709, 3365
BsaBI	GATNNNNATC	1 cut(s)	2652
BsaI	GGTCTC	3 cut(s)	1281, 1743, 1776
BsaWI	WCCGGW	1 cut(s)	188
BsaXI	ACNNNNNCTCC	2 cut(s)	68, 98
Bsc4I	CCNNNNNNNGG	5 cut(s)	1853, 1966, 2689, 3437, 3668
Bse118I	RCCGGY	2 cut(s)	194, 1657
Bse21I	CCTNAGG	1 cut(s)	2466
Bse8I	GATNNNNATC	1 cut(s)	2652
BseBI	CCWGG	7 cut(s)	232, 378, 1560, 1613, 1787, 2718, 3432
BseCI	ATCGAT	1 cut(s)	69
BseJI	GATNNNNATC	1 cut(s)	2652
BseLI	CCNNNNNNNGG	5 cut(s)	1853, 1966, 2689, 3437, 3668
BseMII	CTCAG	8 cut(s)	374, 1458, 2099, 2370, 2480, 2612, 2679, 3243
BseRI	GAGGAG	2 cut(s)	359, 2586
BseSI	GKGCMC	2 cut(s)	1918, 3488
BseXI	GCAGC	8 cut(s)	247, 425, 656, 727, 961, 1159, 1927, 2146
BseYI	CCCAGC	1 cut(s)	1661
BsgI	GTGCAG	2 cut(s)	517, 2731
Bsh1236I	CGCG	2 cut(s)	98, 888
Bsh1285I	CGRYCG	1 cut(s)	142
BshNI	GGYRCC	3 cut(s)	496, 2613, 3473
BshVI	ATCGAT	1 cut(s)	69
BsiEI	CGRYCG	1 cut(s)	142
BsiHKAI	GWGCWC	5 cut(s)	351, 361, 1918, 3019, 3123
BsiHKCI	CYCGRG	3 cut(s)	176, 2004, 3154
BsiSI	CCGG	6 cut(s)	17, 189, 195, 493, 1658, 1776
BsiWI	CGTACG	1 cut(s)	93
BslFI	GGGAC	4 cut(s)	167, 1744, 2484, 3015
BslI	CCNNNNNNNGG	5 cut(s)	1853, 1966, 2689, 3437, 3668
BsmAI	GTCTC	9 cut(s)	133, 186, 390, 1281, 1743, 1776, 2375, 3053, 3199
BsmBI	CGTCTC	2 cut(s)	133, 390
BsmFI	GGGAC	4 cut(s)	167, 1744, 2484, 3015
BsmI	GAATGC	2 cut(s)	1582, 2884
Bso31I	GGTCTC	3 cut(s)	1281, 1743, 1776
BsoBI	CYCGRG	3 cut(s)	176, 2004, 3154
Bsp1286I	GDGCHC	7 cut(s)	351, 361, 1776, 1918, 3019, 3123, 3488
Bsp1720I	GCTNAGC	1 cut(s)	360
Bsp19I	CCATGG	1 cut(s)	819
Bsp68I	TCGCGA	1 cut(s)	888
BspACI	CCGC	5 cut(s)	55, 98, 324, 327, 2862
BspCNI	CTCAG	8 cut(s)	373, 1459, 2100, 2371, 2479, 2611, 2678, 3244
BspDI	ATCGAT	1 cut(s)	69
BspFNI	CGCG	2 cut(s)	98, 888
BspHI	TCATGA	1 cut(s)	114
BspLI	GGNNCC	7 cut(s)	193, 498, 817, 841, 2615, 3475, 3673
BspMAI	CTGCAG	2 cut(s)	1906, 2164
BspPI	GGATC	7 cut(s)	302, 603, 702, 2418, 2456, 2648, 2728
BspT107I	GGYRCC	3 cut(s)	496, 2613, 3473
BspTI	CTTAAG	2 cut(s)	1009, 2618
BspTNI	GGTCTC	3 cut(s)	1281, 1743, 1776
BsrFI	RCCGGY	2 cut(s)	194, 1657
BssAI	RCCGGY	2 cut(s)	194, 1657
BssSI	CACGAG	2 cut(s)	674, 698
BssT1I	CCWWGG	6 cut(s)	294, 819, 2097, 2982, 3526, 3742
Bst2BI	CACGAG	2 cut(s)	674, 698
Bst2UI	CCWGG	7 cut(s)	232, 378, 1560, 1613, 1787, 2718, 3432
Bst4CI	ACNGT	4 cut(s)	2129, 2280, 2417, 3006
Bst6I	CTCTTC	6 cut(s)	216, 336, 660, 1249, 2779, 3318
BstAFI	CTTAAG	2 cut(s)	1009, 2618
BstAPI	GCANNNNNTGC	1 cut(s)	3551
BstBAI	YACGTR	2 cut(s)	2709, 3365
BstC8I	GCNNGC	8 cut(s)	513, 886, 1299, 1659, 1712, 1848, 2495, 3358
BstDSI	CCRYGG	2 cut(s)	319, 819
BstFNI	CGCG	2 cut(s)	98, 888
BstHHI	GCGC	1 cut(s)	103
BstMAI	GTCTC	9 cut(s)	133, 186, 390, 1281, 1743, 1776, 2375, 3053, 3199
BstMCI	CGRYCG	1 cut(s)	142
BstNI	CCWGG	7 cut(s)	232, 378, 1560, 1613, 1787, 2718, 3432
BstNSI	RCATGY	2 cut(s)	3269, 3276
BstPAI	GACNNNNGTC	1 cut(s)	1735
BstSFI	CTRYAG	4 cut(s)	1176, 1902, 2160, 2856
BstSLI	GKGCMC	2 cut(s)	1918, 3488
BstUI	CGCG	2 cut(s)	98, 888
BstV1I	GCAGC	8 cut(s)	247, 425, 656, 727, 961, 1159, 1927, 2146
BstV2I	GAAGAC	1 cut(s)	1666
BstX2I	RGATCY	3 cut(s)	694, 2588, 2653
BstXI	CCANNNNNNTGG	1 cut(s)	1208
BstYI	RGATCY	3 cut(s)	694, 2588, 2653
Bsu15I	ATCGAT	1 cut(s)	69
Bsu36I	CCTNAGG	1 cut(s)	2466
BsuTUI	ATCGAT	1 cut(s)	69
BtgI	CCRYGG	2 cut(s)	319, 819
BtgZI	GCGATG	2 cut(s)	298, 903
BtrI	CACGTC	1 cut(s)	2298
BtsI	GCAGTG	4 cut(s)	160, 513, 1133, 1899
BtsIMutI	CAGTG	9 cut(s)	160, 361, 513, 959, 1133, 1899, 1929, 2855, 3076
BtuMI	TCGCGA	1 cut(s)	888
Cac8I	GCNNGC	8 cut(s)	513, 886, 1299, 1659, 1712, 1848, 2495, 3358
CciI	TCATGA	1 cut(s)	114
CfoI	GCGC	1 cut(s)	103
Cfr10I	RCCGGY	2 cut(s)	194, 1657
Cfr13I	GGNCC	9 cut(s)	380, 840, 1003, 1536, 1556, 2788, 2845, 3244, 3739
ClaI	ATCGAT	1 cut(s)	69
DraI	TTTAAA	2 cut(s)	622, 2151
DraIII	CACNNNGTG	1 cut(s)	1066
Eam1104I	CTCTTC	6 cut(s)	216, 336, 660, 1249, 2779, 3318
EarI	CTCTTC	6 cut(s)	216, 336, 660, 1249, 2779, 3318
Eco130I	CCWWGG	6 cut(s)	294, 819, 2097, 2982, 3526, 3742
Eco147I	AGGCCT	1 cut(s)	680
Eco24I	GRGCYC	1 cut(s)	1776
Eco31I	GGTCTC	3 cut(s)	1281, 1743, 1776
Eco47I	GGWCC	4 cut(s)	840, 1003, 2788, 3739
Eco57I	CTGAAG	5 cut(s)	641, 1088, 1463, 1829, 3441
Eco72I	CACGTG	1 cut(s)	2709
Eco81I	CCTNAGG	1 cut(s)	2466
Eco88I	CYCGRG	3 cut(s)	176, 2004, 3154
EcoO109I	RGGNCCY	1 cut(s)	1003
EcoRI	GAATTC	2 cut(s)	1453, 2025
EcoRII	CCWGG	7 cut(s)	230, 376, 1558, 1611, 1785, 2716, 3430
EcoT14I	CCWWGG	6 cut(s)	294, 819, 2097, 2982, 3526, 3742
EcoT22I	ATGCAT	3 cut(s)	436, 2086, 3556
EcoT38I	GRGCYC	1 cut(s)	1776
ErhI	CCWWGG	6 cut(s)	294, 819, 2097, 2982, 3526, 3742
Esp3I	CGTCTC	2 cut(s)	133, 390
FalI	AAGNNNNNCTT	4 cut(s)	2045, 2077, 2511, 2543
FaqI	GGGAC	4 cut(s)	167, 1744, 2484, 3015
FauNDI	CATATG	2 cut(s)	1281, 2245
FblI	GTMKAC	1 cut(s)	3681
FriOI	GRGCYC	1 cut(s)	1776
FspBI	CTAG	4 cut(s)	519, 944, 2385, 3209
GlaI	GCGC	1 cut(s)	102
GsaI	CCCAGC	1 cut(s)	1665
GsuI	CTGGAG	2 cut(s)	2977, 3711
HapII	CCGG	6 cut(s)	17, 189, 195, 493, 1658, 1776
HhaI	GCGC	1 cut(s)	103
Hin6I	GCGC	1 cut(s)	101
HinP1I	GCGC	1 cut(s)	101
HincII	GTYRAC	2 cut(s)	2989, 3682
HindII	GTYRAC	2 cut(s)	2989, 3682
HindIII	AAGCTT	3 cut(s)	581, 2287, 3770
HpaI	GTTAAC	1 cut(s)	2989
HpaII	CCGG	6 cut(s)	17, 189, 195, 493, 1658, 1776
HphI	GGTGA	4 cut(s)	1094, 1437, 2123, 3491
Hpy166II	GTNNAC	6 cut(s)	631, 976, 1916, 2427, 2989, 3682
Hpy8I	GTNNAC	6 cut(s)	631, 976, 1916, 2427, 2989, 3682
Hpy99I	CGWCG	1 cut(s)	3683
HpyCH4III	ACNGT	4 cut(s)	2129, 2280, 2417, 3006
HpyCH4IV	ACGT	8 cut(s)	627, 2167, 2218, 2297, 2708, 3364, 3678, 3796
HpySE526I	ACGT	8 cut(s)	627, 2167, 2218, 2297, 2708, 3364, 3678, 3796
HspAI	GCGC	1 cut(s)	101
KpnI	GGTACC	2 cut(s)	2617, 3477
KroI	GCCGGC	1 cut(s)	1657
KroNI	GCCGGC	1 cut(s)	1659
KspAI	GTTAAC	1 cut(s)	2989
LmnI	GCTCC	9 cut(s)	20, 224, 346, 421, 815, 1507, 1937, 2195, 3022
Lsp1109I	GCAGC	8 cut(s)	247, 425, 656, 727, 961, 1159, 1927, 2146
MaeI	CTAG	4 cut(s)	519, 944, 2385, 3209
MaeII	ACGT	8 cut(s)	627, 2167, 2218, 2297, 2708, 3364, 3678, 3796
MaeIII	GTNAC	9 cut(s)	1082, 1222, 1443, 1685, 2129, 2367, 2725, 3268, 3497
MfeI	CAATTG	2 cut(s)	2763, 2821
MflI	RGATCY	3 cut(s)	694, 2588, 2653
MhlI	GDGCHC	7 cut(s)	351, 361, 1776, 1918, 3019, 3123, 3488
MlyI	GAGTC	4 cut(s)	132, 188, 2777, 3411
MmeI	TCCRAC	7 cut(s)	586, 688, 995, 1055, 1309, 3582, 3699
Mph1103I	ATGCAT	3 cut(s)	436, 2086, 3556
MroNI	GCCGGC	1 cut(s)	1657
MroXI	GAANNNNTTC	2 cut(s)	1969, 3312
MslI	CAYNNNNRTG	4 cut(s)	71, 354, 1126, 1206
MspCI	CTTAAG	2 cut(s)	1009, 2618
MspI	CCGG	6 cut(s)	17, 189, 195, 493, 1658, 1776
MunI	CAATTG	2 cut(s)	2763, 2821
Mva1269I	GAATGC	2 cut(s)	1582, 2884
MvaI	CCWGG	7 cut(s)	232, 378, 1560, 1613, 1787, 2718, 3432
MvnI	CGCG	2 cut(s)	98, 888
NaeI	GCCGGC	1 cut(s)	1659
NciI	CCSGG	3 cut(s)	18, 494, 1776
NcoI	CCATGG	1 cut(s)	819
NdeI	CATATG	2 cut(s)	1281, 2245
NgoMIV	GCCGGC	1 cut(s)	1657
NlaIV	GGNNCC	7 cut(s)	193, 498, 817, 841, 2615, 3475, 3673
NmeAIII	GCCGAG	1 cut(s)	1985
NmuCI	GTSAC	6 cut(s)	1082, 1222, 1443, 2129, 2367, 3497
NruI	TCGCGA	1 cut(s)	888
NsiI	ATGCAT	3 cut(s)	436, 2086, 3556
NspI	RCATGY	2 cut(s)	3269, 3276
OliI	CACNNNNGTG	2 cut(s)	354, 1126
PaeR7I	CTCGAG	1 cut(s)	176
PagI	TCATGA	1 cut(s)	114
PceI	AGGCCT	1 cut(s)	680
PciI	ACATGT	1 cut(s)	3265
PcsI	WCGNNNNNNNCGW	5 cut(s)	47, 66, 174, 681, 2173
PctI	GAATGC	2 cut(s)	1582, 2884
PdiI	GCCGGC	1 cut(s)	1659
PdmI	GAANNNNTTC	2 cut(s)	1969, 3312
Pfl23II	CGTACG	1 cut(s)	93
PfoI	TCCNGGA	4 cut(s)	16, 1611, 2716, 3430
Ple19I	CGATCG	1 cut(s)	142
PleI	GAGTC	4 cut(s)	131, 187, 2776, 3410
PmaCI	CACGTG	1 cut(s)	2709
PmlI	CACGTG	1 cut(s)	2709
PpsI	GAGTC	4 cut(s)	131, 187, 2776, 3410
Ppu21I	YACGTR	2 cut(s)	2709, 3365
PpuMI	RGGWCCY	1 cut(s)	1003
PscI	ACATGT	1 cut(s)	3265
PshAI	GACNNNNGTC	1 cut(s)	1735
PshBI	ATTAAT	1 cut(s)	798
PsiI	TTATAA	1 cut(s)	1587
Psp1406I	AACGTT	1 cut(s)	2218
Psp5II	RGGWCCY	1 cut(s)	1003
Psp6I	CCWGG	7 cut(s)	230, 376, 1558, 1611, 1785, 2716, 3430
PspCI	CACGTG	1 cut(s)	2709
PspFI	CCCAGC	1 cut(s)	1661
PspGI	CCWGG	7 cut(s)	230, 376, 1558, 1611, 1785, 2716, 3430
PspLI	CGTACG	1 cut(s)	93
PspN4I	GGNNCC	7 cut(s)	193, 498, 817, 841, 2615, 3475, 3673
PspPI	GGNCC	9 cut(s)	380, 840, 1003, 1536, 1556, 2788, 2845, 3244, 3739
PspPPI	RGGWCCY	1 cut(s)	1003
PspXI	VCTCGAGB	1 cut(s)	176
PstI	CTGCAG	2 cut(s)	1906, 2164
PsuI	RGATCY	3 cut(s)	694, 2588, 2653
PvuI	CGATCG	1 cut(s)	142
RruI	TCGCGA	1 cut(s)	888
RseI	CAYNNNNRTG	4 cut(s)	71, 354, 1126, 1206
SalI	GTCGAC	1 cut(s)	3680
Sau96I	GGNCC	9 cut(s)	380, 840, 1003, 1536, 1556, 2788, 2845, 3244, 3739
ScaI	AGTACT	2 cut(s)	1492, 3160
SchI	GAGTC	4 cut(s)	132, 188, 2777, 3411
SduI	GDGCHC	7 cut(s)	351, 361, 1776, 1918, 3019, 3123, 3488
SfcI	CTRYAG	4 cut(s)	1176, 1902, 2160, 2856
Sfr274I	CTCGAG	1 cut(s)	176
SinI	GGWCC	4 cut(s)	840, 1003, 2788, 3739
SlaI	CTCGAG	1 cut(s)	176
SmiMI	CAYNNNNRTG	4 cut(s)	71, 354, 1126, 1206
SmlI	CTYRAG	6 cut(s)	176, 459, 1009, 1264, 2517, 2618
SmoI	CTYRAG	6 cut(s)	176, 459, 1009, 1264, 2517, 2618
SseBI	AGGCCT	1 cut(s)	680
SsiI	CCGC	5 cut(s)	55, 98, 324, 327, 2862
SspI	AATATT	2 cut(s)	565, 3327
SspMI	CTAG	4 cut(s)	519, 944, 2385, 3209
StuI	AGGCCT	1 cut(s)	680
StyI	CCWWGG	6 cut(s)	294, 819, 2097, 2982, 3526, 3742
TaaI	ACNGT	4 cut(s)	2129, 2280, 2417, 3006
TaiI	ACGT	8 cut(s)	630, 2170, 2221, 2300, 2711, 3367, 3681, 3799
TaqII	GACCGA	1 cut(s)	320
TatI	WGTACW	5 cut(s)	547, 1490, 1804, 3158, 3730
TauI	GCSGC	4 cut(s)	101, 327, 330, 2864
TscAI	CASTG	9 cut(s)	167, 361, 513, 966, 1133, 1906, 1936, 2855, 3076
TseFI	GTSAC	6 cut(s)	1082, 1222, 1443, 2129, 2367, 3497
TseI	GCWGC	8 cut(s)	235, 413, 669, 740, 949, 1172, 1940, 2159
Tsp45I	GTSAC	6 cut(s)	1082, 1222, 1443, 2129, 2367, 3497
TspGWI	ACGGA	2 cut(s)	458, 3040
TspRI	CASTG	9 cut(s)	167, 361, 513, 966, 1133, 1906, 1936, 2855, 3076
Vha464I	CTTAAG	2 cut(s)	1009, 2618
VneI	GTGCAC	1 cut(s)	1914
VpaK11BI	GGWCC	4 cut(s)	840, 1003, 2788, 3739
VspI	ATTAAT	1 cut(s)	798
XceI	RCATGY	2 cut(s)	3269, 3276
XcmI	CCANNNNNNNNNTGG	2 cut(s)	850, 1619
XhoI	CTCGAG	1 cut(s)	176
XmiI	GTMKAC	1 cut(s)	3681
XmnI	GAANNNNTTC	2 cut(s)	1969, 3312
XspI	CTAG	4 cut(s)	519, 944, 2385, 3209
ZrmI	AGTACT	2 cut(s)	1492, 3160
Zsp2I	ATGCAT	3 cut(s)	436, 2086, 3556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.