Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004306

Physical Location & Seq

Genomic Coordinates Forward (+)

40046695 .. 40056734

Gene Structure 10040 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1283500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3825 bp

ATGGTGTCTGCAACTCCCGGAAGAAACCCTAACCCTACCACTAAAACCTCCAAAAATGGGTACCAAATTGGAGGCGTCCAGGTGGAGTTCCCGTACCACCCGTACGGTGCCCAGCTGGCATTCATGAGCAGGGTCATCTCCACTCTCGATCGAGCTCACAGAGACGGTCATTGCCACGCATTGCTCGAGTCTCCGACCGGAACCGGAAAATCGCTTTCTCTTCTCTGCTCCACTCTCGCTTGGCAACAGGCTTATAAGTCCAAGAATCAGTTCGCCAATCTCTTGCATTCCAAGCCCGATCCCAAGGCCATGACCGATCCGGCGGCTCACGGCGGCGGATTTGTTCCCGAAGAGGAACCTTCAAGTGTTCAGTTATCGGAGAAGGCAAAGACAAAGCAATCCTTGGCAAACAATAAGGATGAAAAGAAAAAGGCAACACCTACCATATACTACGCATCGAGGACACACTCACAAATTTCTCAAGTGATACGTGAGTATCGGAAAACTTCCTACCGGGTGCCTATGGCAGTGCTGGCCTCAAGGAAGCATTACTGCACAAATGCACAAGTACATGGGAAAGAGAATATTGACGAAGAATGTAAGCTGCTTTTGAAGAACCGAGAGGTTGGATGCTTAGAATTTAAAAATGTAAACAAAGTCAAAGGCCATCCATCACTTCAGAAAGGAGGCTGCCATGAGGCCCATGATATTGAAGATCTTGTAAAAGTTGGAAAATCTGTGAAAGGTTGTTCATATTATGCAGCACGATCTTTGTCAGATGATGCAGAATTGGTTTTTTGCCCATATAACTACATCATTAATCCAGTAATTCGGGGAGCAATGGAAGTAGATATTAAGGGATCCATTGTTGTTCTCGATGAAGCCCACAATATGGAAGACGTTGCTCGTGATGCTGGTAGTGTTGATATTGAAGAAGACATTTTGCACAAATTGCAGATAGAACTAGAGCAGCTTTGCCCAGTTGACGTTTTGGTATACCAACCTCTATACGAAACAATACAGGGCCTCATAAGTTGGATTGAGCAAAGGAAAAATAAGTTGGAGAAGCGTGAATTTCAGTACTATGTCTCTTGTTGGACTGGCGATAAGGCTTTGAGAGAGCTCCAAGAAGCTAATGTATCACAGCAGTGCTTCCCAATCTTACTTGAATGTGCTGTGAAGGCATTCAAAGCTGCTACAGATACAGAATCAGATATACCACATTTAAGTGGCATGGCTGTTGTGATATTGGAAGGATTGTTCTCTTCGCTTACTTACTTTTTCTCAAGAAATGGGTGTCATATGGTTGATTTTCAGCTTGCTTTACAACGATATGTTAAAAGAGATGCTGGAGCTTCTGGCGATTGGACGCATACCTTAAGTTTGTGGTGTTTGAATCCTGCTGTTGTGTTTAAAGATATTGCTGATCTTTCCTTGTCAGTCATTTTAACATCAGGGACTCTGTCACCTTTGAATTCCTTTTCATCTGAGCTTGGTCTTCAGTTTGGAACAACTCTGGAAGCTCCACATGTTATTGATGTTGAATCACAAGTTTGGCCCGCTGTTATTTCTACTGGCCCAGGTAACTTACCATTGAATGCAAGTTATAAGACAGCAGATGAATATGCCTTCCAGGATGCACTCGGAAAATCCTTGGAAGAAATTTTCAAGATTGTTCCAGGTGGGTGTCTTGTGTTCTTCCCAAGTTATAAGCTGATGGGAAAACTATGCAAGCGTTGGCATGGAACAGGACAATGGTCTCAGTTAAATGAAAAAAAGAACCTTTTTGTTGAGCCAAGAGGAGGAGACCAGGAGGAATTTGATGCTGTTTTGAAGGGTTATTATGATTCAATTTGTAAGGGTAATAAACCAGTTTTTGGGGCAAAGAAAAGTGCCAACAAGATAGCAAAAAAAAGTCACTTCGCTGCGTTCAAGTGTCCACGAAAGAGTAACATTGATGGAGCTGCTTTTCTTGCTGTGTGCCGAGGAAAGGTTTCAGAAGGAATTGATTTTTCAGATGACAATGCTCGGGCAGTGATAATTGTCGGGATTCCGTTTCCCAACATAAATGATATTCAAGTTGTCTTAAAGAAGAAGTATAATGATACACATAAGTCATCCAAAAGCCTTTTGAGTGGGAACGAGTGGTATTGCCAGCAAGCCTTTCGAGCTCTAAATCAAGCTGCAGGACGTTGCATTCGCCATAGGTTTGATTATGGAGCTGTCATCTTATTAGATGAACGCTATCACAAGGAAAGAAATACAGCATATGTGTCAAAGTGGCTAAAGAAATCCATCAGACATTATAACAACTTTGATACGTCAATGGAGGAATTAAAATCCTTTTTCAGTGATGTCAAGGAAAGAGTAAGCAAGAATATGGTTAATGTTTCACAGAGGTTTGAAGCTAAAGAGGAAATTATTCACTCTATGGGTCAAAATGAAGGAATTACAAAAGTGAAATATCAGAAATTAAAGAAGTTCGATTGTTGCAAACGACCAGAGAATTCTGAAATGAAGCATGATGGCCAACTGGCATCTCAAGGTTGTATGGAAGCTCAGCAATTTGTGCGAATGGATGTGGATGCTGACCATAGCAAGGAAATAATTGATCTAGATTGTTGTCCTCAGAAAAATTTAGGGTACCCAAAGCCATCTTTGTCCATGGCATTCTCTGATGCAGATCCAGACATCTCCATTGTCAAAGAAATCCCTAATATGGATGGAATTGGTTCCGTATACAGTCCAGGATCCTTAAATAAGGATGAGAATTCTGGTTTAACTTCAATCACAATTGAGGCTTCTTCTCATGAAATGTCATTTCAATCAATAGCCTTGACCAATAGTAGCAGAGCTTCTCCAAATGCCCAGTGCAGCCTTGTAGCAACTCCTAAAAAGAATGTGACTTTGAAGATGCACAGCTCGATACTAGAAACAGAATCATCTTTGAATTTGAGTGTTAATTCTCATACCCAAAAGAGGAGAAAGTCAATGTATTCACCACTGGTCAACTTCGTTAATGGACAGTCTGATACTCCTGATTCTGAAACTCCAGATTCTACTAGTTCTGTAAATTACTCAATGGCTAGCAGAGATGCGAATCGCAAAATTGAATTTAGTGTTGAGACTAATGGTTCAGAACACAAATCTAAGTCCAATGTTCCTCATGTTCTCACGACTAATATTTGTGTTGCTTCATCCACGTCAGCTGGTTGTGTAATGGATAAAAGACTACAAATTTCTTGCTCACTCTGCAAAACCCCGTTGGGACTTCCTGAGAATCAGCTACATCTAACATGCTCTTTGTCTTCTTCGTCGAAATGTCATTTAGAATCTCTTGGTACGAATGTATTAAAGTTTCACACAGGAAATACATCCTCATCAGGAATACCTGTTATTATAACTGACAGTGCATCAGTTGATCAGAGGCTCTTGAATTCTGAAGGTGCTACTGGACAGGGCATATGGTGTGAAGAGGATGGATGTGTTTTCAGTACCATCTTCTGCCCCTTCTGCAGTAGCCACAACAATTGCCTTGGTGTACGTGTCATGGCAGCTAACAGGTCAAATGTTCATTTACTCAACAAGATACTGTTTTACTTAGATCAGTTGCATATTCAAAATCTTAAAGCATCTAGTGATAGCGCTCCAGAAGACAAGGATCTCTTGCCTGCAGTTGATTCAAGTAGGGATAAAGCCTCCATTCTAAATTCCTTTGATAGATTCTCATACTCGCCGCAAGAGCAGAACATGGGAGGCTGGAGGACTACAAAATCAAAGCTTAAACTACCCAAAAGACGCCTACTTCCTGAAGCAGAGGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003678 GO:0003824 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

DEAD_2 Helicase_C_2

Protein Analysis

1275

Amino Acids

141.28

Weight (kDa)

7.87

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	151 - 314	1.4e-43	DEAD_2
Helicase_C_2	PF13307	553 - 764	2e-52	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20720 AT1G20720 AT1G20750
fragaria_vesca	FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570 FvH4_5g25570
malus_domestica	MD16G1283500.v1.1
prunus_persica	Prupe.5G009400_v2.0.a1 Prupe.5G009400_v2.0.a1 Prupe.5G009400_v2.0.a1
pyrus_communis	pycom16g26050
rosa_chinensis	RchiOBHm_Chr7g0215861
rosa_multiflora	Rmu_co7960896.1_g000001 Rmu_sc0004689.1_g000011 Rmu_sc0009085.1_g000007
rosa_roxburghii	Rroxscaffold_3G00243840
rosa_samantha	Rh7AG294400 Rh7CG314400 Rh7DG297200
rosa_wichuraiana	Rw7G024990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	5 cut(s)	255, 1608, 1710, 2307, 3401
Acc65I	GGTACC	2 cut(s)	60, 2643
AccB1I	GGYRCC	4 cut(s)	60, 107, 517, 2643
AccB7I	CCANNNNNTGG	2 cut(s)	892, 1877
AccI	GTMKAC	2 cut(s)	996, 2739
AciI	CCGC	5 cut(s)	323, 333, 336, 1560, 3737
AclWI	GGATC	8 cut(s)	293, 311, 855, 868, 2678, 2745, 2758, 3669
AcoI	YGGCCR	1 cut(s)	2527
AcuI	CTGAAG	3 cut(s)	662, 1484, 3462
AcyI	GRCGYC	2 cut(s)	75, 3799
AfaI	GTAC	9 cut(s)	62, 95, 104, 570, 1082, 2645, 3343, 3496, 3543
AfeI	AGCGCT	1 cut(s)	3646
AfiI	CCNNNNNNNGG	8 cut(s)	57, 892, 1802, 1877, 1990, 2599, 2719, 2979
AflII	CTTAAG	1 cut(s)	1378
AflIII	ACRYGT	2 cut(s)	1528, 3544
AhlI	ACTAGT	1 cut(s)	3062
AjiI	CACGTC	1 cut(s)	3204
AjnI	CCWGG	6 cut(s)	78, 1579, 1632, 1678, 1809, 2746
AjuI	GAANNNNNNNTTGG	4 cut(s)	254, 286, 1043, 1075
AleI	CACNNNNGTG	1 cut(s)	1147
AloI	GAACNNNNNNTCC	2 cut(s)	1244, 1276
Alw21I	GWGCWC	3 cut(s)	157, 1125, 2173
Alw26I	GTCTC	6 cut(s)	156, 195, 1093, 1764, 1800, 3119
AlwI	GGATC	8 cut(s)	293, 311, 855, 868, 2678, 2745, 2758, 3669
Ama87I	CYCGRG	2 cut(s)	185, 2028
Aor51HI	AGCGCT	1 cut(s)	3646
AoxI	GGCC	8 cut(s)	306, 534, 664, 699, 1024, 1556, 1576, 2527
ArsI	GACNNNNNNTTYG	2 cut(s)	2181, 2213
AseI	ATTAAT	1 cut(s)	819
Asp700I	GAANNNNTTC	5 cut(s)	269, 1184, 1993, 2421, 2731
Asp718I	GGTACC	2 cut(s)	60, 2643
AspLEI	GCGC	1 cut(s)	3647
AspS9I	GGNCC	4 cut(s)	700, 1024, 1557, 1577
AsuC2I	CCSGG	2 cut(s)	18, 515
AsuHPI	GGTGA	2 cut(s)	1458, 2991
AsuNHI	GCTAGC	1 cut(s)	3086
AvaI	CYCGRG	2 cut(s)	185, 2028
BaeGI	GKGCMC	1 cut(s)	112
BaeI	ACNNNNGTAYC	6 cut(s)	479, 479, 512, 512, 3333, 3366
BalI	TGGCCA	1 cut(s)	2529
BamHI	GGATCC	2 cut(s)	860, 2750
BanI	GGYRCC	4 cut(s)	60, 107, 517, 2643
BanII	GRGCYC	3 cut(s)	157, 1125, 2173
BarI	GAAGNNNNNNTAC	2 cut(s)	2839, 2871
BauI	CACGAG	1 cut(s)	906
BbsI	GAAGAC	5 cut(s)	903, 942, 1490, 3300, 3660
Bbv12I	GWGCWC	3 cut(s)	157, 1125, 2173
BceAI	ACGGC	1 cut(s)	346
BcgI	CGANNNNNNTGC	2 cut(s)	2147, 2181
BciT130I	CCWGG	6 cut(s)	80, 1581, 1634, 1680, 1811, 2748
BclI	TGATCA	1 cut(s)	3421
BcnI	CCSGG	2 cut(s)	18, 515
BcoDI	GTCTC	6 cut(s)	156, 195, 1093, 1764, 1800, 3119
BcuI	ACTAGT	1 cut(s)	3062
BfaI	CTAG	6 cut(s)	965, 2615, 2930, 3063, 3087, 3636
BfmI	CTRYAG	4 cut(s)	1197, 2184, 3514, 3672
BfoI	RGCGCY	1 cut(s)	3648
BfrI	CTTAAG	1 cut(s)	1378
BglI	GCCNNNNNGGC	1 cut(s)	116
BglII	AGATCT	1 cut(s)	715
BlpI	GCTNAGC	1 cut(s)	2559
BmcAI	AGTACT	1 cut(s)	1082
Bme1390I	CCNGG	8 cut(s)	18, 80, 515, 1581, 1634, 1680, 1811, 2748
BmeT110I	CYCGRG	2 cut(s)	185, 2028
BmgBI	CACGTC	1 cut(s)	3204
BmgT120I	GGNCC	4 cut(s)	700, 1024, 1557, 1577
BmiI	GGNNCC	9 cut(s)	62, 109, 202, 357, 519, 862, 2645, 2734, 2752
BmrFI	CCNGG	8 cut(s)	18, 80, 515, 1581, 1634, 1680, 1811, 2748
BmrI	ACTGGG	2 cut(s)	974, 2863
BmtI	GCTAGC	1 cut(s)	3090
BmuI	ACTGGG	2 cut(s)	974, 2863
BoxI	GACNNNNGTC	1 cut(s)	1756
BpiI	GAAGAC	5 cut(s)	903, 942, 1490, 3300, 3660
BpmI	CTGGAG	4 cut(s)	1371, 3036, 3633, 3781
Bpu1102I	GCTNAGC	1 cut(s)	2559
BpuEI	CTTGAG	4 cut(s)	465, 523, 1270, 2526
BpuMI	CCSGG	2 cut(s)	18, 515
BsaAI	YACGTR	2 cut(s)	491, 3545
BsaBI	GATNNNNATC	2 cut(s)	2682, 3099
BsaHI	GRCGYC	2 cut(s)	75, 3799
BsaI	GGTCTC	2 cut(s)	1764, 1800
BsaJI	CCNNGG	7 cut(s)	303, 402, 1579, 1653, 1984, 2664, 3535
BsaWI	WCCGGW	2 cut(s)	197, 203
BsaXI	ACNNNNNCTCC	2 cut(s)	77, 107
Bsc4I	CCNNNNNNNGG	8 cut(s)	57, 892, 1802, 1877, 1990, 2599, 2719, 2979
Bse1I	ACTGG	9 cut(s)	824, 980, 1105, 1579, 1871, 2538, 2869, 3009, 3457
Bse3DI	GCAATG	3 cut(s)	169, 179, 846
Bse8I	GATNNNNATC	2 cut(s)	2682, 3099
BseBI	CCWGG	6 cut(s)	80, 1581, 1634, 1680, 1811, 2748
BseDI	CCNNGG	7 cut(s)	303, 402, 1579, 1653, 1984, 2664, 3535
BseJI	GATNNNNATC	2 cut(s)	2682, 3099
BseLI	CCNNNNNNNGG	8 cut(s)	57, 892, 1802, 1877, 1990, 2599, 2719, 2979
BseMI	GCAATG	3 cut(s)	169, 179, 846
BseMII	CTCAG	5 cut(s)	1479, 1775, 2573, 2642, 3267
BseNI	ACTGG	9 cut(s)	824, 980, 1105, 1579, 1871, 2538, 2869, 3009, 3457
BseRI	GAGGAG	3 cut(s)	1815, 1818, 2995
BseSI	GKGCMC	1 cut(s)	112
BseYI	CCCAGC	1 cut(s)	111
BsgI	GTGCAG	2 cut(s)	538, 2893
Bsh1285I	CGRYCG	2 cut(s)	151, 198
BshFI	GGCC	8 cut(s)	308, 536, 666, 701, 1026, 1558, 1578, 2529
BshNI	GGYRCC	4 cut(s)	60, 107, 517, 2643
BsiEI	CGRYCG	2 cut(s)	151, 198
BsiHKAI	GWGCWC	3 cut(s)	157, 1125, 2173
BsiHKCI	CYCGRG	2 cut(s)	185, 2028
BsiSI	CCGG	5 cut(s)	18, 198, 204, 320, 514
BsiWI	CGTACG	1 cut(s)	102
BslFI	GGGAC	2 cut(s)	1471, 3282
BslI	CCNNNNNNNGG	8 cut(s)	57, 892, 1802, 1877, 1990, 2599, 2719, 2979
BsmAI	GTCTC	6 cut(s)	156, 195, 1093, 1764, 1800, 3119
BsmBI	CGTCTC	1 cut(s)	156
BsmFI	GGGAC	2 cut(s)	1471, 3282
BsmI	GAATGC	6 cut(s)	119, 286, 1184, 1603, 2196, 2669
BsnI	GGCC	8 cut(s)	308, 536, 666, 701, 1026, 1558, 1578, 2529
Bso31I	GGTCTC	2 cut(s)	1764, 1800
BsoBI	CYCGRG	2 cut(s)	185, 2028
Bsp1286I	GDGCHC	4 cut(s)	112, 157, 1125, 2173
Bsp1720I	GCTNAGC	1 cut(s)	2559
Bsp19I	CCATGG	1 cut(s)	2664
BspACI	CCGC	5 cut(s)	323, 333, 336, 1560, 3737
BspANI	GGCC	8 cut(s)	308, 536, 666, 701, 1026, 1558, 1578, 2529
BspCNI	CTCAG	5 cut(s)	1480, 1774, 2572, 2641, 3268
BspHI	TCATGA	2 cut(s)	123, 2809
BspLI	GGNNCC	9 cut(s)	62, 109, 202, 357, 519, 862, 2645, 2734, 2752
BspMAI	CTGCAG	3 cut(s)	2188, 3518, 3676
BspOI	GCTAGC	1 cut(s)	3090
BspPI	GGATC	8 cut(s)	293, 311, 855, 868, 2678, 2745, 2758, 3669
BspT107I	GGYRCC	4 cut(s)	60, 107, 517, 2643
BspTI	CTTAAG	1 cut(s)	1378
BspTNI	GGTCTC	2 cut(s)	1764, 1800
BsrDI	GCAATG	3 cut(s)	169, 179, 846
BsrI	ACTGG	9 cut(s)	824, 980, 1105, 1579, 1871, 2538, 2869, 3009, 3457
BssECI	CCNNGG	7 cut(s)	303, 402, 1579, 1653, 1984, 2664, 3535
BssNAI	GTATAC	2 cut(s)	997, 2740
BssNI	GRCGYC	2 cut(s)	75, 3799
BssSI	CACGAG	1 cut(s)	906
BssT1I	CCWWGG	5 cut(s)	303, 402, 1653, 2664, 3535
Bst1107I	GTATAC	2 cut(s)	997, 2740
Bst2BI	CACGAG	1 cut(s)	906
Bst2UI	CCWGG	6 cut(s)	80, 1581, 1634, 1680, 1811, 2748
Bst4CI	ACNGT	6 cut(s)	107, 167, 2744, 3027, 3410, 3594
Bst6I	CTCTTC	4 cut(s)	225, 345, 1270, 3468
BstACI	GRCGYC	2 cut(s)	75, 3799
BstAFI	CTTAAG	1 cut(s)	1378
BstAPI	GCANNNNNTGC	2 cut(s)	952, 2569
BstBAI	YACGTR	2 cut(s)	491, 3545
BstC8I	GCNNGC	9 cut(s)	117, 534, 1320, 1560, 1733, 2156, 2160, 3088, 3672
BstDEI	CTNAG	8 cut(s)	634, 1488, 1761, 2559, 2628, 3150, 3276, 3601
BstDSI	CCRYGG	1 cut(s)	2664
BstH2I	RGCGCY	1 cut(s)	3648
BstHHI	GCGC	1 cut(s)	3647
BstMAI	GTCTC	6 cut(s)	156, 195, 1093, 1764, 1800, 3119
BstMCI	CGRYCG	2 cut(s)	151, 198
BstNI	CCWGG	6 cut(s)	80, 1581, 1634, 1680, 1811, 2748
BstNSI	RCATGY	2 cut(s)	1532, 3300
BstPAI	GACNNNNGTC	1 cut(s)	1756
BstSCI	CCNGG	8 cut(s)	16, 78, 513, 1579, 1632, 1678, 1809, 2746
BstSFI	CTRYAG	4 cut(s)	1197, 2184, 3514, 3672
BstSLI	GKGCMC	1 cut(s)	112
BstV2I	GAAGAC	5 cut(s)	903, 942, 1490, 3300, 3660
BstX2I	RGATCY	5 cut(s)	715, 860, 2683, 2750, 3661
BstYI	RGATCY	5 cut(s)	715, 860, 2683, 2750, 3661
BstZ17I	GTATAC	2 cut(s)	997, 2740
BsuRI	GGCC	8 cut(s)	308, 536, 666, 701, 1026, 1558, 1578, 2529
BtgI	CCRYGG	1 cut(s)	2664
BtrI	CACGTC	1 cut(s)	3204
BtsI	GCAGTG	3 cut(s)	534, 1154, 2040
BtsIMutI	CAGTG	7 cut(s)	534, 1154, 2040, 2356, 2876, 3002, 3415
Cac8I	GCNNGC	9 cut(s)	117, 534, 1320, 1560, 1733, 2156, 2160, 3088, 3672
CciI	TCATGA	2 cut(s)	123, 2809
CfoI	GCGC	1 cut(s)	3647
Cfr13I	GGNCC	4 cut(s)	700, 1024, 1557, 1577
CseI	GACGC	3 cut(s)	64, 1378, 3807
Csp6I	GTAC	9 cut(s)	61, 94, 103, 569, 1081, 2644, 3342, 3495, 3542
CspCI	CAANNNNNGTGG	2 cut(s)	3512, 3547
CviQI	GTAC	9 cut(s)	61, 94, 103, 569, 1081, 2644, 3342, 3495, 3542
DdeI	CTNAG	8 cut(s)	634, 1488, 1761, 2559, 2628, 3150, 3276, 3601
DraI	TTTAAA	2 cut(s)	643, 1414
EaeI	YGGCCR	1 cut(s)	2527
Eam1104I	CTCTTC	4 cut(s)	225, 345, 1270, 3468
EarI	CTCTTC	4 cut(s)	225, 345, 1270, 3468
EciI	GGCGGA	1 cut(s)	351
Ecl136II	GAGCTC	3 cut(s)	155, 1123, 2171
Eco130I	CCWWGG	5 cut(s)	303, 402, 1653, 2664, 3535
Eco24I	GRGCYC	3 cut(s)	157, 1125, 2173
Eco31I	GGTCTC	2 cut(s)	1764, 1800
Eco47III	AGCGCT	1 cut(s)	3646
Eco53kI	GAGCTC	3 cut(s)	155, 1123, 2171
Eco57I	CTGAAG	3 cut(s)	662, 1484, 3462
Eco88I	CYCGRG	2 cut(s)	185, 2028
EcoICRI	GAGCTC	3 cut(s)	155, 1123, 2171
EcoO109I	RGGNCCY	1 cut(s)	1024
EcoRI	GAATTC	4 cut(s)	1474, 2506, 2770, 3436
EcoRII	CCWGG	6 cut(s)	78, 1579, 1632, 1678, 1809, 2746
EcoT14I	CCWWGG	5 cut(s)	303, 402, 1653, 2664, 3535
EcoT38I	GRGCYC	3 cut(s)	157, 1125, 2173
ErhI	CCWWGG	5 cut(s)	303, 402, 1653, 2664, 3535
Esp3I	CGTCTC	1 cut(s)	156
FalI	AAGNNNNNCTT	4 cut(s)	386, 418, 3650, 3682
FaqI	GGGAC	2 cut(s)	1471, 3282
FauI	CCCGC	1 cut(s)	1567
FauNDI	CATATG	3 cut(s)	1302, 2269, 3464
FbaI	TGATCA	1 cut(s)	3421
FblI	GTMKAC	2 cut(s)	996, 2739
FriOI	GRGCYC	3 cut(s)	157, 1125, 2173
FspBI	CTAG	6 cut(s)	965, 2615, 2930, 3063, 3087, 3636
GlaI	GCGC	1 cut(s)	3646
GsaI	CCCAGC	1 cut(s)	115
GsuI	CTGGAG	4 cut(s)	1371, 3036, 3633, 3781
HaeII	RGCGCY	1 cut(s)	3648
HaeIII	GGCC	8 cut(s)	308, 536, 666, 701, 1026, 1558, 1578, 2529
HapII	CCGG	5 cut(s)	18, 198, 204, 320, 514
HgaI	GACGC	3 cut(s)	64, 1378, 3807
HhaI	GCGC	1 cut(s)	3647
Hin1I	GRCGYC	2 cut(s)	75, 3799
Hin6I	GCGC	1 cut(s)	3645
HinP1I	GCGC	1 cut(s)	3645
HincII	GTYRAC	2 cut(s)	985, 3010
HindII	GTYRAC	2 cut(s)	985, 3010
HindIII	AAGCTT	1 cut(s)	3779
HpaII	CCGG	5 cut(s)	18, 198, 204, 320, 514
HphI	GGTGA	2 cut(s)	1458, 2991
Hpy166II	GTNNAC	7 cut(s)	652, 985, 997, 1940, 2740, 3010, 3542
Hpy8I	GTNNAC	7 cut(s)	652, 985, 997, 1940, 2740, 3010, 3542
Hpy99I	CGWCG	1 cut(s)	3319
HpyCH4III	ACNGT	6 cut(s)	107, 167, 2744, 3027, 3410, 3594
HpyCH4IV	ACGT	7 cut(s)	490, 900, 987, 2191, 2321, 3203, 3544
HpyF3I	CTNAG	8 cut(s)	634, 1488, 1761, 2559, 2628, 3150, 3276, 3601
HpySE526I	ACGT	7 cut(s)	490, 900, 987, 2191, 2321, 3203, 3544
Hsp92I	GRCGYC	2 cut(s)	75, 3799
HspAI	GCGC	1 cut(s)	3645
KpnI	GGTACC	2 cut(s)	64, 2647
Ksp22I	TGATCA	1 cut(s)	3421
LmnI	GCTCC	8 cut(s)	233, 836, 1128, 1352, 1528, 1961, 2219, 3652
MaeI	CTAG	6 cut(s)	965, 2615, 2930, 3063, 3087, 3636
MaeII	ACGT	7 cut(s)	490, 900, 987, 2191, 2321, 3203, 3544
MaeIII	GTNAC	5 cut(s)	1464, 1583, 1916, 1949, 2902
MfeI	CAATTG	2 cut(s)	2793, 3529
MflI	RGATCY	5 cut(s)	715, 860, 2683, 2750, 3661
MhlI	GDGCHC	4 cut(s)	112, 157, 1125, 2173
MlsI	TGGCCA	1 cut(s)	2529
MluNI	TGGCCA	1 cut(s)	2529
MlyI	GAGTC	2 cut(s)	197, 1453
MmeI	TCCRAC	6 cut(s)	218, 607, 709, 1016, 1041, 1076
Mox20I	TGGCCA	1 cut(s)	2529
MroXI	GAANNNNTTC	5 cut(s)	269, 1184, 1993, 2421, 2731
MscI	TGGCCA	1 cut(s)	2529
MslI	CAYNNNNRTG	2 cut(s)	1147, 1227
Msp20I	TGGCCA	1 cut(s)	2529
MspA1I	CMGCKG	3 cut(s)	115, 1562, 3209
MspCI	CTTAAG	1 cut(s)	1378
MspI	CCGG	5 cut(s)	18, 198, 204, 320, 514
MspR9I	CCNGG	8 cut(s)	18, 80, 515, 1581, 1634, 1680, 1811, 2748
MunI	CAATTG	2 cut(s)	2793, 3529
Mva1269I	GAATGC	6 cut(s)	119, 286, 1184, 1603, 2196, 2669
MvaI	CCWGG	6 cut(s)	80, 1581, 1634, 1680, 1811, 2748
NciI	CCSGG	2 cut(s)	18, 515
NcoI	CCATGG	1 cut(s)	2664
NdeI	CATATG	3 cut(s)	1302, 2269, 3464
NheI	GCTAGC	1 cut(s)	3086
NlaIV	GGNNCC	9 cut(s)	62, 109, 202, 357, 519, 862, 2645, 2734, 2752
NmeAIII	GCCGAG	1 cut(s)	2009
NmuCI	GTSAC	3 cut(s)	1464, 1916, 2902
NspI	RCATGY	2 cut(s)	1532, 3300
OliI	CACNNNNGTG	1 cut(s)	1147
PaeR7I	CTCGAG	1 cut(s)	185
PagI	TCATGA	2 cut(s)	123, 2809
PciI	ACATGT	1 cut(s)	1528
PcsI	WCGNNNNNNNCGW	1 cut(s)	183
PctI	GAATGC	6 cut(s)	119, 286, 1184, 1603, 2196, 2669
PdmI	GAANNNNTTC	5 cut(s)	269, 1184, 1993, 2421, 2731
Pfl23II	CGTACG	1 cut(s)	102
PflFI	GACNNNGTC	1 cut(s)	1462
PflMI	CCANNNNNTGG	2 cut(s)	892, 1877
PfoI	TCCNGGA	3 cut(s)	16, 1632, 2746
Ple19I	CGATCG	1 cut(s)	151
PleI	GAGTC	2 cut(s)	196, 1453
PpsI	GAGTC	2 cut(s)	196, 1453
Ppu21I	YACGTR	2 cut(s)	491, 3545
PscI	ACATGT	1 cut(s)	1528
PshAI	GACNNNNGTC	1 cut(s)	1756
PshBI	ATTAAT	1 cut(s)	819
PsiI	TTATAA	5 cut(s)	255, 1608, 1710, 2307, 3401
Psp124BI	GAGCTC	3 cut(s)	157, 1125, 2173
Psp6I	CCWGG	6 cut(s)	78, 1579, 1632, 1678, 1809, 2746
PspFI	CCCAGC	1 cut(s)	111
PspGI	CCWGG	6 cut(s)	78, 1579, 1632, 1678, 1809, 2746
PspLI	CGTACG	1 cut(s)	102
PspN4I	GGNNCC	9 cut(s)	62, 109, 202, 357, 519, 862, 2645, 2734, 2752
PspPI	GGNCC	4 cut(s)	700, 1024, 1557, 1577
PspXI	VCTCGAGB	1 cut(s)	185
PstI	CTGCAG	3 cut(s)	2188, 3518, 3676
PsuI	RGATCY	5 cut(s)	715, 860, 2683, 2750, 3661
PsyI	GACNNNGTC	1 cut(s)	1462
PvuI	CGATCG	1 cut(s)	151
PvuII	CAGCTG	2 cut(s)	115, 3209
RsaI	GTAC	9 cut(s)	62, 95, 104, 570, 1082, 2645, 3343, 3496, 3543
RsaNI	GTAC	9 cut(s)	61, 94, 103, 569, 1081, 2644, 3342, 3495, 3542
RseI	CAYNNNNRTG	2 cut(s)	1147, 1227
SacI	GAGCTC	3 cut(s)	157, 1125, 2173
Sau96I	GGNCC	4 cut(s)	700, 1024, 1557, 1577
ScaI	AGTACT	1 cut(s)	1082
SchI	GAGTC	2 cut(s)	197, 1453
ScrFI	CCNGG	8 cut(s)	18, 80, 515, 1581, 1634, 1680, 1811, 2748
SduI	GDGCHC	4 cut(s)	112, 157, 1125, 2173
SfcI	CTRYAG	4 cut(s)	1197, 2184, 3514, 3672
Sfr274I	CTCGAG	1 cut(s)	185
SlaI	CTCGAG	1 cut(s)	185
SmiMI	CAYNNNNRTG	2 cut(s)	1147, 1227
SmlI	CTYRAG	6 cut(s)	185, 480, 538, 1285, 1378, 2541
SmoI	CTYRAG	6 cut(s)	185, 480, 538, 1285, 1378, 2541
SpeI	ACTAGT	1 cut(s)	3062
SsiI	CCGC	5 cut(s)	323, 333, 336, 1560, 3737
SspI	AATATT	2 cut(s)	586, 3184
SspMI	CTAG	6 cut(s)	965, 2615, 2930, 3063, 3087, 3636
SstI	GAGCTC	3 cut(s)	157, 1125, 2173
StyD4I	CCNGG	8 cut(s)	16, 78, 513, 1579, 1632, 1678, 1809, 2746
StyI	CCWWGG	5 cut(s)	303, 402, 1653, 2664, 3535
TaaI	ACNGT	6 cut(s)	107, 167, 2744, 3027, 3410, 3594
TaiI	ACGT	7 cut(s)	493, 903, 990, 2194, 2324, 3206, 3547
TaqI	TCGA	9 cut(s)	147, 151, 186, 458, 876, 2167, 2484, 2924, 3317
TaqII	GACCGA	1 cut(s)	329
TatI	WGTACW	2 cut(s)	568, 1080
TauI	GCSGC	3 cut(s)	326, 336, 3739
TscAI	CASTG	7 cut(s)	534, 1154, 2040, 2356, 2876, 3009, 3415
TseFI	GTSAC	3 cut(s)	1464, 1916, 2902
Tsp45I	GTSAC	3 cut(s)	1464, 1916, 2902
TspGWI	ACGGA	2 cut(s)	2043, 2725
TspRI	CASTG	7 cut(s)	534, 1154, 2040, 2356, 2876, 3009, 3415
Tth111I	GACNNNGTC	1 cut(s)	1462
Van91I	CCANNNNNTGG	2 cut(s)	892, 1877
Vha464I	CTTAAG	1 cut(s)	1378
VspI	ATTAAT	1 cut(s)	819
XbaI	TCTAGA	1 cut(s)	2614
XceI	RCATGY	2 cut(s)	1532, 3300
XhoI	CTCGAG	1 cut(s)	185
XmiI	GTMKAC	2 cut(s)	996, 2739
XmnI	GAANNNNTTC	5 cut(s)	269, 1184, 1993, 2421, 2731
XspI	CTAG	6 cut(s)	965, 2615, 2930, 3063, 3087, 3636
ZrmI	AGTACT	1 cut(s)	1082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.