FvH4_5g25650

TLR4 regulator and MIR-interacting MSAP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011086
Physical Location & Seq
Forward (+)
17170738 .. 17173066
2329 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25650.t2

Sequence Viewer

Length: 564 bp
ATGGAAAGGTCAAAGGTCGCCACTGTGATTCTGATATTCACCATCTTCACCGTCGTTTCTTCCATCGACGACAAATGCGCCGCCTGCAATGCCGTCGCGGAAGAGCTAGAATGGGGAATTTCCAAGGAAAAGCCCAGAAACCACTTGGATATGAGACACCGGTTGGACTCCCAAGGTCAACGGCGAGGAAAGGTAATCGACTACAGAGTCAGTGAGCTCAGAGTTGTTGAACTCCTTGATGGACTTTGTGACAAGATGCAGGATTACACTCTTAGCAAGATAGATTCAGACAGACCAGTTTGGATCAAAGTGCGTGATTGGGATAACCTTACGATAGTTTTAGATAAACAAGAAGCTAGAGCATATTCAAAGGATATATCAACTTATTGTGGAAGGTTACTTGAGGAAACAGAAGATGAGATTGCAGACTTGATAAAGAAAGGATCTTTAGGAGTAGGAGATGTAAGCAAGGTTCTATGTCAGGATCTGAGCAAACACTGCAGCAAAACAAGTGCATTGGATGAGGAAAATGGATCTAACGGTGGATCAGATGGAGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3456

Protein Analysis

188

Amino Acids

21.0

Weight (kDa)

5.01

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3456 PF11938 2 - 117 2.9e-26 TLR4 regulator and MIR-interacting MSAP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42480 AT1G42480 AT1G42480
fragaria_vesca FvH4_5g25650 FvH4_5g25650
malus_domestica MD06G1006600.v1.1
prunus_persica Prupe.5G008600_v2.0.a1 Prupe.5G008700_v2.0.a1
pyrus_communis pycom06g00580
rosa_chinensis RchiOBHm_Chr7g0215941
rosa_laevigata RLG00000002605
rosa_multiflora Rmu_co8213036.1_g000001 Rmu_sc0030911.1_g000001
rosa_roxburghii Rroxscaffold_3G00243760
rosa_rugosa Rorug07G0157200
rosa_samantha Rh7AG295200 Rh7BG287200 Rh7CG315300 Rh7DG297900
rosa_wichuraiana Rw7G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AccII CGCG 1 cut(s) 98
AciI CCGC 2 cut(s) 81, 98
AclWI GGATC 5 cut(s) 311, 451, 492, 541, 553
AcsI RAATTY 1 cut(s) 117
AgeI ACCGGT 1 cut(s) 159
AgsI TTSAA 2 cut(s) 230, 369
AluBI AGCT 3 cut(s) 106, 217, 356
AluI AGCT 3 cut(s) 106, 217, 356
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 5 cut(s) 311, 451, 492, 541, 553
AlwNI CAGNNNCTG 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 501
ApoI RAATTY 1 cut(s) 117
AsiGI ACCGGT 1 cut(s) 159
AspLEI GCGC 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 31, 40
BanII GRGCYC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 513
BccI CCATC 4 cut(s) 50, 71, 233, 545
BceAI ACGGC 2 cut(s) 77, 197
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 2 cut(s) 107, 357
BfmI CTRYAG 2 cut(s) 202, 499
BisI GCNGC 2 cut(s) 81, 502
BlsI GCNGC 2 cut(s) 82, 503
BmsI GCATC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 123, 172
BsaWI WCCGGW 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 94
BseDI CCNNGG 2 cut(s) 123, 172
BseGI GGATG 1 cut(s) 526
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 2 cut(s) 232, 479
BseNI ACTGG 1 cut(s) 296
BseXI GCAGC 1 cut(s) 513
Bsh1236I CGCG 1 cut(s) 98
BshTI ACCGGT 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 5 cut(s) 303, 443, 484, 533, 545
BspACI CCGC 2 cut(s) 81, 98
BspCNI CTCAG 2 cut(s) 231, 480
BspFNI CGCG 1 cut(s) 98
BspMAI CTGCAG 1 cut(s) 503
BspPI GGATC 5 cut(s) 311, 451, 492, 541, 553
BspQI GCTCTTC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 2 cut(s) 123, 172
BssMI GATC 5 cut(s) 303, 443, 484, 533, 545
BssT1I CCWWGG 2 cut(s) 123, 172
Bst4CI ACNGT 3 cut(s) 25, 52, 542
Bst6I CTCTTC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 3 cut(s) 218, 272, 488
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 1 cut(s) 98
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 5 cut(s) 306, 446, 487, 536, 548
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 5 cut(s) 303, 443, 484, 533, 545
BstMWI GCNNNNNNNGC 3 cut(s) 84, 89, 498
BstSFI CTRYAG 2 cut(s) 202, 499
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 1 cut(s) 513
BstX2I RGATCY 3 cut(s) 443, 484, 533
BstYI RGATCY 3 cut(s) 443, 484, 533
BtsCI GGATG 1 cut(s) 526
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 3 cut(s) 21, 217, 496
Cac8I GCNNGC 1 cut(s) 85
CaiI CAGNNNCTG 1 cut(s) 487
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 159
CspAI ACCGGT 1 cut(s) 159
CviJI RGCY 4 cut(s) 106, 133, 217, 356
CviKI_1 RGCY 4 cut(s) 106, 133, 217, 356
DdeI CTNAG 3 cut(s) 218, 272, 488
DpnI GATC 5 cut(s) 305, 445, 486, 535, 547
DpnII GATC 5 cut(s) 303, 443, 484, 533, 545
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 123, 172
Eco24I GRGCYC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 217
EcoT14I CCWWGG 2 cut(s) 123, 172
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 2 cut(s) 123, 172
FaiI YATR 4 cut(s) 152, 364, 377, 478
Fnu4HI GCNGC 2 cut(s) 81, 502
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 2 cut(s) 81, 502
FspBI CTAG 2 cut(s) 107, 357
GlaI GCGC 1 cut(s) 79
GluI GCNGC 2 cut(s) 81, 502
HapII CCGG 1 cut(s) 160
HhaI GCGC 1 cut(s) 80
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 179
HindII GTYRAC 1 cut(s) 179
HinfI GANTC 4 cut(s) 28, 167, 207, 284
HpaII CCGG 1 cut(s) 160
HphI GGTGA 2 cut(s) 31, 40
Hpy166II GTNNAC 1 cut(s) 179
Hpy188I TCNGA 6 cut(s) 33, 221, 289, 489, 550, 563
Hpy188III TCNNGA 1 cut(s) 482
Hpy8I GTNNAC 1 cut(s) 179
Hpy99I CGWCG 3 cut(s) 56, 71, 98
HpyAV CCTTC 1 cut(s) 387
HpyCH4III ACNGT 3 cut(s) 25, 52, 542
HpyCH4V TGCA 5 cut(s) 87, 259, 425, 501, 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 89, 498
HpyF3I CTNAG 3 cut(s) 218, 272, 488
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 5 cut(s) 303, 443, 484, 533, 545
LguI GCTCTTC 1 cut(s) 96
LpnPI CCDG 6 cut(s) 97, 148, 173, 245, 309, 467
Lsp1109I GCAGC 1 cut(s) 513
LweI GCATC 1 cut(s) 246
MaeI CTAG 2 cut(s) 107, 357
MaeIII GTNAC 2 cut(s) 248, 396
MalI GATC 5 cut(s) 305, 445, 486, 535, 547
MboI GATC 5 cut(s) 303, 443, 484, 533, 545
MboII GAAGA 4 cut(s) 37, 51, 113, 425
MflI RGATCY 3 cut(s) 443, 484, 533
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 1 cut(s) 117
MlyI GAGTC 2 cut(s) 161, 216
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 3 cut(s) 179, 397, 517
MspI CCGG 1 cut(s) 160
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 84, 89, 498
NdeII GATC 5 cut(s) 303, 443, 484, 533, 545
NmuCI GTSAC 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 96
PfeI GAWTC 2 cut(s) 28, 284
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 2 cut(s) 82, 503
PleI GAGTC 2 cut(s) 161, 215
PpsI GAGTC 2 cut(s) 161, 215
Psp124BI GAGCTC 1 cut(s) 219
PsrI GAACNNNNNNTAC 2 cut(s) 456, 488
PstI CTGCAG 1 cut(s) 503
PstNI CAGNNNCTG 1 cut(s) 487
PsuI RGATCY 3 cut(s) 443, 484, 533
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 96
SatI GCNGC 2 cut(s) 81, 502
Sau3AI GATC 5 cut(s) 303, 443, 484, 533, 545
SchI GAGTC 2 cut(s) 161, 216
SduI GDGCHC 1 cut(s) 219
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 2 cut(s) 202, 499
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 1 cut(s) 117
SsiI CCGC 2 cut(s) 81, 98
SspMI CTAG 2 cut(s) 107, 357
SstI GAGCTC 1 cut(s) 219
StyI CCWWGG 2 cut(s) 123, 172
TaaI ACNGT 3 cut(s) 25, 52, 542
TaqI TCGA 2 cut(s) 66, 198
TasI AATT 1 cut(s) 117
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 28, 284
TscAI CASTG 3 cut(s) 28, 217, 503
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 1 cut(s) 501
Tsp45I GTSAC 1 cut(s) 248
TspRI CASTG 3 cut(s) 28, 217, 503
XapI RAATTY 1 cut(s) 117
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XspI CTAG 2 cut(s) 107, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.